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lysyl oxidase

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https://www.readbyqxmd.com/read/29028628/role-of-pdgf-bb-in-proliferation-differentiation-and-maintaining-stem-cell-properties-of-pdl-cells-in-vitro
#1
Zornitsa Mihaylova, Rozaliya Tsikandelova, Pavel Sanimirov, Natalia Gateva, Vanyo Mitev, Nikolay Ishkitiev
OBJECTIVE: Platelet-derived growth factor-BB (PDGF-BB) is one of the most abundant growth factors in platelet derived products and has been shown to stimulate regeneration after tissue injury. There is a population of mesenchymal stem cells (MSC) in human periodontal ligament (PDL) which can contribute to tissue regeneration under appropriate conditions. DESIGN: PDL cells were isolated and characterized using stem cell and differentiation markers via immunofluorescence and flow cytometry and then cultured in vitro and treated with different concentrations of PDGF-BB...
September 25, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28986701/mechano-growth-factor-e-peptide-inhibits-invasion-of-melanoma-cells-and-up-regulates-chop-expression-via-endoplasmic-reticulum-stress
#2
Jianhua He, Lili Dong, Kang Xu, Yuna Qian, Chunli Wang, Yongqiang Sha, Juila Li Zhong, Wanqian Liu, Yonggang Lv, Yang Song, Li Yang
OBJECTIVE: To evaluate the effects of mechano growth factor E peptide (MGF) on the invasive properties of melanoma cells. RESULTS: Melanoma cells (GLL19) were treated with 10, 20 and 30 ng MGF/ml for 24 h. Their invasive properties were investigated by transwell assay. Cytoskeleton reorganization was assessed via staining with phalloidin-FITC; lysyl oxidase (LOX) family gene expression was tested by qRT-PCR, and western blotting was used to detect expression of the matrix metalloproteinases (MMPs) and endoplasmic reticulum (ER) stress...
October 6, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28970517/human-in-vitro-model-reveals-the-effects-of-collagen-cross-linking-on-keratoconus-pathogenesis
#3
Rabab Sharif, Jesper Hjortdal, Henrik Sejersen, Garett Frank, Dimitrios Karamichos
Keratoconus (KC) is a corneal thinning disorder that leads to severe vision impairment As opposed to corneal transplantation; corneal collagen crosslinking (CXL) is a relatively non-invasive procedure that leads to an increase in corneal stiffness. In order to evaluate the effect of CXL on human corneal stromal cells in vitro, we developed a 3-D in vitro CXL model, using primary Human corneal fibroblasts (HCFs) from healthy patients and Human Keratoconus fibroblasts (HKCs) from KC patients. Cells were plated on transwell polycarbonate membranes and stimulated by a stable vitamin C...
October 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28966236/lysyl-oxidase-enhances-the-deposition-of-tropoelastin-through-the-catalysis-of-tropoelastin-molecules-on-the-cell-surface
#4
Fumiaki Sato, Ryo Seino-Sudo, Mami Okada, Hiroyasu Sakai, Tetsuro Yumoto, Hiroshi Wachi
The cross-linking of elastin by lysyl oxidase (LOX) family members is essential for the integrity and elasticity of elastic fibers, which play an important role in the characteristic resilience of various tissues. However, the temporal sequence of oxidation by LOX during elastic fiber formation is still incompletely understood. Here, we demonstrate that the cross-linking of tropoelastin molecules by LOX occurs concurrent with elastin deposition. Our data show that LOX deficiency or the inhibition of LOX enzyme activity leads to the loss of elastin deposition in skin fibroblast...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28965583/the-copper-dependent-lysyl-oxidases-contribute-to-the-pathogenesis-of-pulmonary-emphysema-in-chronic-obstructive-pulmonary-disease-patients
#5
Neziha Besiktepe, Ozgecan Kayalar, Ezel Ersen, Fusun Oztay
Abnormalities in the elastic fiber biology are seen in pulmonary emphysema (PE). The copper-dependent lysyl oxidases regulate the production and accumulation of elastic fibers in the connective tissue. This study focused on the relationship between lysyl oxidase (LOX), LOX-like protein 1 (LOXL1), and LOXL2 and PE pathogenesis. Lung samples with or without PE from patients with chronic obstructive lung disease (n=35) were used. Protein levels of elastin, LOX, LOXL1, LOXL2, hypoxia inducible factor 1-alpha (HIF-1α), copper metabolism domain containing-1 (COMMD1), and phosphatase and tensin homolog (PTEN) were assayed using microscopic and biochemical methods The emphysematous areas were characterized by enlargement of the alveoli, destruction of the alveolar structure, accumulation of macrophages in the alveolar lumens, and showed increased HIF-1α immunoreactivity...
December 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/28963018/temporal-development-of-near-native-functional-properties-and-correlations-with-qmri-in-self-assembling-fibrocartilage-treated-with-exogenous-loxl2
#6
Pasha Hadidi, Derek D Cissell, Jerry C Hu, Kyriacos A Athanasiou
Advances in cartilage tissue engineering have led to constructs with mechanical integrity and biochemical composition increasingly resembling that of native tissues. In particular, collagen cross-linking with lysyl oxidase has been used to significantly enhance the mechanical properties of engineered neotissues. In this study, development of collagen cross-links over time, and correlations with tensile properties, were examined in self-assembling neotissues. Additionally, quantitative MRI metrics were examined in relation to construct mechanical properties as well as pyridinoline cross-link content and other engineered tissue components...
September 26, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28951129/ras-inhibitors-display-an-anti-metastatic-effect-by-downregulation-of-lysyl-oxidase-through-inhibition-of-the-ras-pi3k-akt-hif-1%C3%AE-pathway
#7
Yoko Yoshikawa, Osamu Takano, Ichiro Kato, Yoshihisa Takahashi, Fumi Shima, Tohru Kataoka
Metastasis stands as the major obstacle for the survival from cancers. Nonetheless most existing anti-cancer drugs inhibit only cell proliferation, and discovery of agents having both anti-proliferative and anti-metastatic properties would be more beneficial. We previously reported the discovery of small-molecule Ras inhibitors, represented by Kobe0065, that displayed anti-proliferative activity on xenografts of human colorectal cancer (CRC) cell line SW480 carrying the K-ras(G12V)gene. Here we show that treatment of cancer cells carrying the activated ras genes with Kobe0065 or a siRNA targeting Ras downregulates the expression of lysyl oxidase (LOX), which has been implicated in metastasis...
September 23, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28947950/nuclear-expression-of-lysyl-oxidase-enzyme-is-an-independent-prognostic-factor-in-rectal-cancer-patients
#8
Na Liu, Thomas R Cox, Weiyingqi Cui, Gunnar Adell, Birgitta Holmlund, Jie Ping, Ingvar Jarlsfelt, Janine T Erler, Xiao-Feng Sun
Emerging evidence has implicated a pivotal role for lysyl oxidase (LOX) in cancer progression and metastasis. Whilst the majority of work has focused on the extracellular matrix cross-linking role of LOX, the exact function of intracellular LOX localisation remains unclear. In this study, we analysed the LOX expression patterns in the nuclei of rectal cancer patient samples and determined the clinical significance of this expression. Nuclear LOX expression was significantly increased in patient lymph node metastases compared to their primary tumours...
September 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28947615/vascular-adam17-a-disintegrin-and-metalloproteinase-domain-17-is-required-for-angiotensin-ii-%C3%AE-aminopropionitrile-induced-abdominal-aortic-aneurysm
#9
Tatsuo Kawai, Takehiko Takayanagi, Steven J Forrester, Kyle J Preston, Takashi Obama, Toshiyuki Tsuji, Tomonori Kobayashi, Michael J Boyer, Hannah A Cooper, Hang Fai Kwok, Tomoki Hashimoto, Rosario Scalia, Victor Rizzo, Satoru Eguchi
Angiotensin II (AngII)-activated epidermal growth factor receptor has been implicated in abdominal aortic aneurysm (AAA) development. In vascular smooth muscle cells (VSMCs), AngII activates epidermal growth factor receptor via a metalloproteinase, ADAM17 (a disintegrin and metalloproteinase domain 17). We hypothesized that AngII-dependent AAA development would be prevented in mice lacking ADAM17 in VSMCs. To test this concept, control and VSMC ADAM17-deficient mice were cotreated with AngII and a lysyl oxidase inhibitor, β-aminopropionitrile, to induce AAA...
September 25, 2017: Hypertension
https://www.readbyqxmd.com/read/28919141/microrna-30a-lysyl-oxidase-axis-in-aortic-dissection-pathogenesis
#10
EDITORIAL
Ming-Sing Si
No abstract text is available yet for this article.
August 30, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28901438/lysyl-oxidase-is-involved-in-synovial-hyperplasia-and-angiogenesis-in-rats-with-collagen%C3%A2-induced-arthritis
#11
Fan Wang, Juan Wan, Qiuyan Li, Mingzhu Zhang, Qiaofeng Wan, Chen Ji, Haibo Li, Rongqing Liu, Mei Han
Lysyl oxidase (LOX) serves an important role in remodeling the extracellular matrix and angiogenesis in various types of cancer; however, whether LOX is involved in the pathogenesis of rheumatoid arthritis remains unknown. In order to investigate this in the present study, β‑aminopropionitrile, an inhibitor of LOX, was injected intraperitoneally into rats with type II collagen‑induced arthritis (CIA). Subsequently, synovial hyperplasia was examined by hematoxyl in and eosin staining, and the microvascular density (MVD) and expression levels of LOX, matrix metalloproteinase (MMP)‑2 and MMP‑9 in the synovial membrane and fluid were determined by immunohistochemistry and ELISA, respectively...
September 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28895470/generation-of-a-close-to-native-in-vitro-system-to-study-lung-cells-ecm-crosstalk
#12
Zuzana Garlikova, Ana Catarina Silva, Ana Rabata, David Potesil, Ivana Ihnatova, Jana Dumkova, Zuzana Koledova, Zbynek Zdrahal, Vladimir Vinarsky, Ales Hample, Perpetua Pinto-do-O, Diana S Nascimento
Extracellular matrix (ECM) is an essential component of the tissue microenvironment, actively shaping cellular behavior. In vitro culture systems are often poor in ECM constituents thus not allowing for naturally occurring cell-ECM interactions. This study reports on a straightforward and efficient method for generation of ECM scaffolds from lung tissue and its subsequent in vitro application using primary lung cells. Mouse lung tissue was subjected to decellularization with 0.2% sodium dodecyl sulfate, hypotonic solutions and DNase...
September 12, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28887577/periostin-and-its-interacting-proteins-in-the-construction-of-extracellular-architectures
#13
REVIEW
Isao Kii, Harumi Ito
Periostin is a matricellular protein that is composed of a multi-domain structure with an amino-terminal EMI domain, a tandem repeat of four FAS 1 domains, and a carboxyl-terminal domain. These distinct domains have been demonstrated to bind to many proteins including extracellular matrix proteins (Collagen type I and V, fibronectin, tenascin, and laminin), matricellular proteins (CCN3 and βig-h3), and enzymes that catalyze covalent crosslinking between extracellular matrix proteins (lysyl oxidase and BMP-1)...
September 8, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28883544/global-fibroblast-activation-throughout-the-left-ventricle-but-localized-fibrosis-after-myocardial-infarction
#14
Chandan K Nagaraju, Eef Dries, Natasa Popovic, Abhishek A Singh, Peter Haemers, H Llewelyn Roderick, Piet Claus, Karin R Sipido, Ronald B Driesen
Fibroblast (Fb) differentiation and interstitial fibrosis contribute to cardiac remodeling and loss of function after myocardial infarction (MI). We investigated regional presence and regulation of fibrosis in a pig MI model. In vivo analysis of regional function and perfusion defined three regions: the scar, the myocardium adjacent to the scar (MIadjacent, reduced function, reduced perfusion reserve), and the remote myocardium (MIremote, minimal functional deficit, maintained perfusion). Interstitial and perivascular fibrosis, and increase of collagen type I, was only observed in the MIadjacent...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28878297/role-of-nf-%C3%AE%C2%BAb-gata3-in-the-inhibition-of-lysyl-oxidase-by-il-1%C3%AE-in-human-amnion-fibroblasts
#15
Chuyue Zhang, Wangsheng Wang, Chao Liu, Jiangwen Lu, Kang Sun
Preterm premature rupture of membranes (pPROM) accounts for one third of preterm births, a leading cause of neonatal death. Understanding the mechanism of membrane rupture is thus of clinical significance in the prevention of preterm birth. Parturition at both term and preterm is associated with increased abundance of proinflammatory cytokines in the fetal membranes regardless of the presence of infection, which is believed to induce rupture of membranes through activation of the matrix metalloproteinases. It remains unknown whether there are any alternative mechanisms underpinning proinflammatory cytokine-induced rupture of membranes...
September 7, 2017: Immunology and Cell Biology
https://www.readbyqxmd.com/read/28875468/multifunctional-jnk-inhibiting-nanotherapeutics-for-augmented-elastic-matrix-regenerative-repair-in-aortic-aneurysms
#16
Andrew Camardo, Dhruv Seshadri, Tom Broekelmann, Robert Mecham, Anand Ramamurthi
Growth of abdominal aortic aneurysms (AAA), localized aortal wall expansions, is driven by the disruption and subsequent loss of aortal wall elastic fibers by matrix metalloproteases (MMPs). Since elastic fibers do not naturally regenerate or repair, arresting/reversing AAA growth has not been possible. Previously, we showed utility of doxycycline (DOX), an MMP inhibitor drug, to stimulate elastic matrix neoassembly and crosslinking at low microgram per milliliter doses in addition to inhibiting MMPs. We currently show in aneurysmal smooth muscle cell (SMC) cultures that effects of exogenous DOX in this dose range are linked to its upregulation of transforming growth factor beta (TGF-β1) via its inhibition of the regulatory protein c-Jun-N-terminal kinase 2 (JNK 2)...
September 5, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28872461/fibroblast-specific-inhibition-of-tgf-%C3%AE-1-signaling-attenuates-lung-and-tumor-fibrosis
#17
Ying Wei, Thomas J Kim, David H Peng, Dana Duan, Don L Gibbons, Mitsuo Yamauchi, Julia R Jackson, Claude J Le Saux, Cheresa Calhoun, Jay Peters, Rik Derynck, Bradley J Backes, Harold A Chapman
TGF-β1 signaling is a critical driver of collagen accumulation and fibrotic disease but also a vital suppressor of inflammation and epithelial cell proliferation. The nature of this multifunctional cytokine has limited the development of global TGF-β1 signaling inhibitors as therapeutic agents. We conducted phenotypic screens for small molecules that inhibit TGF-β1-induced epithelial-mesenchymal transition without immediate TGF-β1 receptor (TβR) kinase inhibition. We identified trihydroxyphenolic compounds as potent blockers of TGF-β1 responses (IC50 ~50 nM), Snail1 expression, and collagen deposition in vivo in models of pulmonary fibrosis and collagen-dependent lung cancer metastasis...
October 2, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28864775/proteolytic-processing-of-lysyl-oxidase-like-2-in-the-extracellular-matrix-is-required-for-crosslinking-of-basement-membrane-collagen-iv
#18
Alberto J López-Jiménez, Trayambak Basak, Roberto M Vanacore
Lysyl oxidase-like-2 (LOXL2) is an enzyme secreted into the extracellular matrix that crosslinks collagens by mediating oxidative deamination of lysine residues. Our previous work demonstrated that this enzyme crosslinks the 7S domain, a structural domain that stabilizes collagen IV scaffolds in the basement membrane. Despite its relevant role in extracellular matrix biosynthesis, little is known about the structural requirements of LOXL2 that enable collagen IV crosslinking. In this study, we demonstrate that LOXL2 is processed extracellularly by serine proteases, generating a 65-kDa form lacking the first two scavenger receptor cysteine-rich domains...
October 13, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28859141/asporin-deficient-mice-have-tougher-skin-and-altered-skin-glycosaminoglycan-content-and-structure
#19
Marco Maccarana, René B Svensson, Anki Knutsson, Antonis Giannopoulos, Mea Pelkonen, MaryAnn Weis, David Eyre, Matthew Warman, Sebastian Kalamajski
The main structural component of connective tissues is fibrillar, cross-linked collagen whose fibrillogenesis can be modulated by Small Leucine-Rich Proteins/Proteoglycans (SLRPs). Not all SLRPs' effects on collagen and extracellular matrix in vivo have been elucidated; one of the less investigated SLRPs is asporin. Here we describe the successful generation of an Aspn-/- mouse model and the investigation of the Aspn-/- skin phenotype. Functionally, Aspn-/- mice had an increased skin mechanical toughness, although there were no structural changes present on histology or immunohistochemistry...
2017: PloS One
https://www.readbyqxmd.com/read/28829846/racial-differences-in-the-extracellular-matrix-and-histone-acetylation-of-the-lamina-cribrosa-and-peripapillary-sclera
#20
Hae-Young Lopilly Park, Jie Hyun Kim, Younhea Jung, Chan Kee Park
Purpose: We investigated the extracellular matrix (ECM) of the lamina cribrosa (LC) and peripapillary sclera (PPS) and compared histone acetylation and related enzymes to identify racial differences between Korean and Caucasian donor eyes. Methods: Posterior segment tissues were obtained from 30 Caucasian donors and 42 age and axial length-matched Korean donors. Histone modification was assessed for histone deacetylase (HDAC) 2, HDAC3, and acetylated histone H3...
August 1, 2017: Investigative Ophthalmology & Visual Science
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