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https://www.readbyqxmd.com/read/28437552/bcr-abl1-transcript-types-showed-distinct-laboratory-characteristics-in-patients-with-chronic-myeloid-leukemia
#1
A P Vasconcelos, I F Azevedo, F C B C Melo, W B Neves, A C A C Azevedo, R A M Melo
In chronic myeloid leukemia (CML) two main types of messenger RNA (e14a2 and e13a2) can be produced by BCR-ABL1 gene rearrangement. Due to conflicting results, the clinical value of these transcripts remains controversial. The aim of this study was to identify associations of e14a2 and e13a2 transcripts with laboratory variables and also the response to treatment. This study included 203 adult patients with CML treated with Imatinib as first-line drug in a reference hematology center in Northeast Brazil. Clinical and laboratory data were obtained after informed consent...
April 20, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28433183/corrigendum-to-comparative-tissue-distribution-and-excretion-study-of-alkaloids-from-herba-ephedrae-radix-aconiti-lateralis-extracts-in-rats-j-pharm-biomed-anal-134-2017-137-142
#2
Mengyue Ren, Shuai Song, Dedong Liang, Weiting Hou, Xiaomei Tan, Jiabo Luo
No abstract text is available yet for this article.
June 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28432818/characterization-of-decellularized-ovine-small-intestine-submucosal-layer-as-extracellular-matrix-based-scaffold-for-tissue-engineering
#3
Morteza Rashtbar, Jamshid Hadjati, Jafar Ai, Issa Jahanzad, Mahmoud Azami, Sadegh Shirian, Somayeh Ebrahimi-Barough, Esmaeil Sadroddiny
Extracellular matrix-based scaffolds derived from mammalian tissues have been used in tissue engineering applications. Among all the tissues, decellularized small intestine submucosal layer (SIS) has been recently investigated for its exceptional characteristics and biocompatibilities. These investigations have been mainly focused on the decellularized porcine SIS; however, there has not been any report on ovine SIS (OSIS) layer. In this study, OSIS was decellularized and its physical, chemical, and morphological properties were evaluated...
April 22, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28429412/bone-extracellular-matrix-hydrogel-enhances-osteogenic-differentiation-of-c2c12-myoblasts-and-mouse-primary-calvarial-cells
#4
Noura Alom, Heather Peto, Glen R Kirkham, Kevin M Shakesheff, Lisa J White
Hydrogel scaffolds derived from the extracellular matrix (ECM) of mammalian tissues have been successfully used to promote tissue repair in vitro and in vivo. The objective of this study was to evaluate the osteogenic potential of ECM hydrogels prepared from demineralized and decellularized bovine bone in the presence and absence of osteogenic medium. Culture of C2C12 and mouse primary calvarial cells (mPCs) on decellularized bone ECM (bECM) and demineralized bone matrix (DBM) gels resulted in increased expression of osteogenic gene markers, including a 3...
April 21, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28428847/lichen-acclimation-to-changing-environments-photobiont-switching-vs-climate-specific-uniqueness-in-psora-decipiens
#5
Laura Williams, Claudia Colesie, Anna Ullmann, Martin Westberg, Mats Wedin, Burkhard Büdel
Unraveling the complex relationship between lichen fungal and algal partners has been crucial in understanding lichen dispersal capacity, evolutionary processes, and responses in the face of environmental change. However, lichen symbiosis remains enigmatic, including the ability of a single fungal partner to associate with various algal partners. Psora decipiens is a characteristic lichen of biological soil crusts (BSCs), across semi-arid, temperate, and alpine biomes, which are particularly susceptible to habitat loss and climate change...
April 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28421508/appendix-d-irvine-scientific-%C3%A2-vitrification-system
#6
Matthew VerMilyea, Amber Brewer
This chapter will describe the use of the Irvine Scientific(®) vitrification and warming solutions (Vit Kits(®)) along with a detailed protocol for the correct use of the CryoTip(®) vitrification device for human embryos and oocytes. Successful pregnancies have been reported after carrying out rapid vitrification methods of oocytes, cleavage stage embryos, and blastocysts using the CryoTip(®) (Popwell et al. Fertil Steril, 101:e20, 2014; Kuwayama et al. Reprod Biomed 11:608-614, 2005; Kuwayama et al. Fertil Steril 84:S187, 2005; Kuwayama et al...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421492/chapter-8-human-oocytes-slow-rate-freezing-methodology
#7
C Zacà, A Borini
Cryopreservation of human oocytes is an important technique for the treatment of human infertility, as it deals successfully with legal, ethical, and moral issues related to embryo cryopreservation (Coticchio et al., Human Fertil (Camb) 4:152-157, 2001; Tucker et al., Eur J Obstet Gynecol Reprod Biol 113: 524-527, 2004) and maintains reproductive potential in women with diseases or conditions that may compromise reproductive capacity. Here, we describe an oocyte cryopreservation technique involving slow freezing-rapid thawing with differential sucrose concentration (0...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28419752/surface-orientation-effects-on-bending-properties-of-surgical-mesh-are-independent-of-tensile-properties
#8
David D Simon, Sharon M Andrews, Rebecca Robinson-Zeigler, Thelma Valdes, Terry O Woods
INTRODUCTION: Current mechanical testing of surgical mesh focuses primarily on tensile properties even though implanted devices are not subjected to pure tensile loads. Our objective was to determine the flexural (bending) properties of surgical mesh and determine if they correlate with mesh tensile properties. METHODS: The flexural rigidity values of 11 different surgical mesh designs were determined along three textile directions (machine, cross-machine, and 45° to machine; n = 5 for each) using ASTM D1388-14 while tracking surface orientation...
April 17, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28419733/interphotoreceptor-matrix-based-biomaterial-impact-on-human-retinal-progenitor-cell-attachment-and-differentiation
#9
Joydip Kundu, Andrew Michaelson, Petr Baranov, Marco Chiumiento, Tom Nigl, Michael J Young, Rebecca L Carrier
While cell transplantation therapies show great promise as treatments for retinal degeneration, the challenge of low cell survival upon transplantation motivates exploration of materials that may serve as cell delivery vehicles and promote survival and differentiation. In this study, we explored the native matrix that surrounds the outer segments of photoreceptors and promotes their homeostasis, interphotoreceptor matrix (IPM), as a substrate for human retinal progenitor cells (hRPCs). Bovine IPM was characterized to determine its structure and biochemical composition, and processed to develop substrates for cells...
April 18, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28419698/collagen-barrier-membranes-do-not-adsorb-hypoxia-mimetic-activity-activity-of-gingival-fibroblasts-cultured-directly-on-collagen-barrier-membranes-loaded-with-hypoxia-mimetic-agents
#10
Diana Al-Habbal, Klara Janjić, Michael Edelmayer, Andreas Moritz, Hermann Agis
Hypoxia-based strategies for applications in oral surgery and periodontology have been proposed where collagen barrier membranes (CBM) are loaded with hypoxia mimetic agents (HMA) to induce a pro-angiogenic response. While it was found that CBM release HMA, it remained unclear if CBM adsorb HMA activity. Here we evaluated the response of oral cells cultured on CBM, supplemented with the HMA dimethyloxalylglycine (DMOG), desferrioxamine (DFO), and l-mimosine (l-MIM). Gingival fibroblasts (GF) were cultured on unwashed CBM as well as on CBM that had been washed with serum-free medium for 48 hours...
April 18, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28419693/ag-loaded-mgsrfe-layered-double-hydroxide-chitosan-composite-scaffold-with-enhanced-osteogenic-and-antibacterial-property-for-bone-engineering-tissue
#11
Dandan Cao, Zhengliang Xu, Yixuan Chen, Qinfei Ke, Changqing Zhang, Yaping Guo
Bone tissue engineering scaffolds for the reconstruction of large bone defects should simultaneously promote osteogenic differentiation and avoid postoperative infection. Herein, we develop, for the first time, Ag-loaded MgSrFe-layered double hydroxide/chitosan (Ag-MgSrFe/CS) composite scaffold. This scaffold exhibits three-dimensional interconnected macroporous structure with a pore size of 100-300 μm. The layered double hydroxide nanoplates in the Ag-MgSrFe/CS show lateral sizes of 200-400 nm and thicknesses of ∼50 nm, and the Ag nanoparticles with particle sizes of ∼20 nm are uniformly dispersed on the scaffold surfaces...
April 17, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28419685/scaffolds-for-epithelial-tissue-engineering-customized-in-elastomeric-molds
#12
Mohamed-Nur Abdallah, Sara Abdollahi, Marco Laurenti, Dongdong Fang, Simon D Tran, Marta Cerruti, Faleh Tamimi
Restoration of soft tissue defects remains a challenge for surgical reconstruction. In this study, we introduce a new approach to fabricate poly(d,l-lactic acid) (PDLLA) scaffolds with anatomical shapes customized to regenerate three-dimensional soft tissue defects. Highly concentrated polymer/salt mixtures were molded in flexible polyether molds. Microcomputed tomography showed that with this approach it was possible to produce scaffolds with clinically acceptable volume ratio maintenance (>90%). Moreover, this technique allowed us to customize the average pore size and pore interconnectivity of the scaffolds by using variations of salt particle size...
April 18, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28417371/analysis-of-complex-carbohydrate-composition-in-plant-cell-wall-using-fourier-transformed-mid-infrared-spectroscopy-ft-ir
#13
Ajay Badhan, Yuxi Wang, Tim A McAllister
Fourier transformed mid-infrared spectroscopy (FTIR) is a powerful tool for compositional analysis of plant cell walls (Acebes et al., Front Plant Sci 5:303, 2014; Badhan et al., Biotechnol Biofuels 7:1-15, 2014; Badhan et al., BioMed Res Int 2015: 562952, 2015; Roach et al., Plant Physiol 156:1351-1363, 2011). The infrared spectrum generates a fingerprint of a sample with absorption peaks corresponding to the frequency of vibrations between the bonds of the atoms making up the material. Here, we describe a method focused on the use of FTIR in combination with principal component analysis (PCA) to characterize the composition of the plant cell wall...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28414883/temperature-and-rainfall-interact-to-control-carbon-cycling-in-tropical-forests
#14
REVIEW
Philip G Taylor, Cory C Cleveland, William R Wieder, Benjamin W Sullivan, Christopher E Doughty, Solomon Z Dobrowski, Alan R Townsend
Tropical forests dominate global terrestrial carbon (C) exchange, and recent droughts in the Amazon Basin have contributed to short-term declines in terrestrial carbon dioxide uptake and storage. However, the effects of longer-term climate variability on tropical forest carbon dynamics are still not well understood. We synthesised field data from more than 150 tropical forest sites to explore how climate regulates tropical forest aboveground net primary productivity (ANPP) and organic matter decomposition, and combined those data with two existing databases to explore climate - C relationships globally...
April 17, 2017: Ecology Letters
https://www.readbyqxmd.com/read/28413848/impact-of-data-model-and-point-density-on-aboveground-forest-biomass-estimation-from-airborne-lidar
#15
Mariano Garcia, Sassan Saatchi, Antonio Ferraz, Carlos Alberto Silva, Susan Ustin, Alexander Koltunov, Heiko Balzter
BACKGROUND: Accurate estimation of aboveground forest biomass (AGB) and its dynamics is of paramount importance in understanding the role of forest in the carbon cycle and the effective implementation of climate change mitigation policies. LiDAR is currently the most accurate technology for AGB estimation. LiDAR metrics can be derived from the 3D point cloud (echo-based) or from the canopy height model (CHM). Different sensors and survey configurations can affect the metrics derived from the LiDAR data...
December 2017: Carbon Balance and Management
https://www.readbyqxmd.com/read/28407434/spatiotemporal-characterization-of-hydration-process-of-asymmetric-polymeric-wound-dressings-for-decubitus-ulcers
#16
Anna Górska, Przemyslaw Dorożyński, Władysław P Węglarz, Krzysztof Jasiński, Mateusz Kurek, Renata Jachowicz, Jolanta Klaja, Piotr Kulinowski
Pressure ulcers belong to the most chalenging clinical problems. As hydration level of such wounds is important for optimal healing, preparation of new wound dressing (WD) materials for pressure ulcers requires thorough in vitro evaluation as prerequisite to final in vivo testing. The aims of the study were to: (a) develop a simple method of preparation of asymmetric polymeric membrane, (b) to propose a set of in vitro methods for membrane characterization during hydration. A polyvinyl alcohol asymmetric membrane with homogeneous skin layer and porous spongy layer was developed with nonadhesive properties and ability to absorb and retain the water...
April 13, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28404508/model-based-analyses-reveal-insular-population-diversification-and-cryptic-frog-species-in-the-ischnocnema-parva-complex-in-the-atlantic-forest-of-brazil
#17
Marcelo Gehara, Adriane Barth, Eliana Faria de Oliveira, Marco Antonio Costa, Célio Fernando Baptista Haddad, Miguel Vences
The Atlantic Forest (AF) of Brazil has long been recognized as a biodiversity conservation hotspot. Despite decades of studies the species inventory of this biome continues to increase with the discovery of cryptic diversity and the description of new species. Different diversification mechanisms have been proposed to explain the diversity in the region, including models of forest dynamics, barriers to gene flow and dispersal. Also, sea level change is thought to have influenced coastal diversification and isolated populations on continental islands...
April 9, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28403375/helminth-fauna-of-leptodactylus-syphax-anura-leptodactylidae-from-caatinga-biome-northeastern-brazil
#18
Aline Gouveia de Souza Lins, Aline Aguiar, Drausio Honorio Morais, Lidiane Aparecida Firmino da Silva, Robson Waldemar Ávila, Reinaldo José da Silva
Leptodactylus syphax is distributed in central, southeastern and northeastern Brazil, eastern Bolivia and southern Paraguay, occupying open areas and rock outcrops, in rock cavities and termite burrows. We collected 21 frogs from the Caatinga region of the state of Ceará, northeastern Brazil, and 7,021 helminths were recovered from 18 of these hosts (overall prevalence = 85.7%). Six helminth taxa were recovered, as follows: Aplectana membranosa (n = 3,756); Schrankiana formosula (n = 3,176); larvae of Physaloptera sp...
January 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/28402086/the-effect-of-increased-microporosity-on-bone-formation-within-silicate-substituted-scaffolds-in-an-ovine-posterolateral-spinal-fusion-model
#19
Melanie Jean Coathup, Gordon William Blunn, Charlie Campion, Chih-Yuan Ho, Karin Angela Hing
This study compared the bone forming capacity of the same formulation of silicate-substituted bone graft substitute materials with different microporosity in an instrumented posterolateral spinal fusion ovine model. Materials with a strut porosity of (i) 22.5% (SiCaP) or (ii) 36.0% (SiCaP(+)) were packed along either side of the spine. Bone apposition rates, % new bone formation, % bone-implant contact, and % graft resorption were quantified at 8, 12, and 24 weeks post surgery. Computed Tomography (CT) was used to grade the formation of fusion bridges between vertebrae...
May 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28400514/role-of-surface-and-subsurface-processes-in-scaling-n2o-emissions-along-riverine-networks
#20
Alessandra Marzadri, Martha M Dee, Daniele Tonina, Alberto Bellin, Jennifer L Tank
Riverine environments, such as streams and rivers, have been reported as sources of the potent greenhouse gas nitrous oxide ([Formula: see text]) to the atmosphere mainly via microbially mediated denitrification. Our limited understanding of the relative roles of the near-surface streambed sediment (hyporheic zone), benthic, and water column zones in controlling [Formula: see text] production precludes predictions of [Formula: see text] emissions along riverine networks. Here, we analyze [Formula: see text] emissions from streams and rivers worldwide of different sizes, morphology, land cover, biomes, and climatic conditions...
April 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
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