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anemia induce in rats

Sreoshi Chatterjee, Nitin Bhardwaj, Rajiv K Saxena
Repeated weekly injections of rat erythrocytes produced autoimmune hemolytic anemia (AIHA) in C57BL/6 mice after 5-6 weeks. Using the double in vivo biotinylation (DIB) technique, recently developed in our laboratory, turnover of erythrocyte cohorts of different age groups during AIHA was monitored. Results indicate a significant decline in the proportion of reticulocytes, young and intermediate age groups of erythrocytes, but a significant increase in the proportion of old erythrocytes in blood circulation...
2016: PloS One
Kuo-Ching Chao, Seu-Hwa Chen, Chun-Chao Chang, Yu-Chieh Lee, Chi-Mei Wang, Jung-Su Chang
SCOPE: Diabetes is associated with increased risks of anemia and activation of advanced glycation end products (AGEs) and the receptor for AGEs (RAGE). However, the effects of pharmacological doses of iron supplementation on AGE metabolism are less clear. The aim was to investigate the effect of ferric citrate supplementation on AGE metabolism. METHODS AND RESULTS: Diabetes was induced in overnight starved rats by intraperitoneal injections of 40 mg/kg streptozotocin and 120 mg/kg nicotinamide...
November 10, 2016: Molecular Nutrition & Food Research
Elaheh Honarkar Shafie, Seyed Ali Keshavarz, Mohammad Esmaiel Kefayati, Fatemeh Taheri, Parvin Sarbakhsh, Mohammad Reza Vafa
BACKGROUND: Ferrous sulfate is the most used supplement for treating anemia, but it can result in unfavorable side effects. Nowadays, nanotechnology is used as a way to increase bioavailability and decrease the side effects of drugs and nutrients. This study investigates the effects of nanoparticles containing iron on blood and inflammatory markers in comparison to ferrous sulfate in anemic rats. METHODS: To induce the model of hemolytic anemia, 50 mg/kg bw phenylhydrazine was injected intraperitoneally in rats on the 1(st) day and 25 mg/kg bw for the four following days...
2016: International Journal of Preventive Medicine
Hua Chen, Gang Cao, Dan-Qian Chen, Ming Wang, Nosratola D Vaziri, Zhi-Hao Zhang, Jia-Rong Mao, Xu Bai, Ying-Yong Zhao
Early detection is critical in prevention and treatment of kidney disease. However currently clinical laboratory and histopathological tests do not provide region-specific and accurate biomarkers for early detection of kidney disease. The present study was conducted to identify sensitive biomarkers for early detection and progression of tubulo-interstitial nephropathy in aristolochic acid I-induced rats at weeks 4, 8 and 12. Biomarkers were validated using aristolochic acid nephropathy (AAN) rats at week 24, adenine-induced chronic kidney disease (CKD) rats and CKD patients...
September 28, 2016: Redox Biology
Paola Ciceri, Francesca Elli, Paola Braidotti, Monica Falleni, Delfina Tosi, Gaetano Bulfamante, Geoffrey A Block, Mario Cozzolino
BACKGROUND AND AIMS: High phosphate-induced vascular calcification (VC) and iron deficiency-induced anemia are two major contributors of cardiovascular morbidity and mortality in patients affected by chronic kidney disease (CKD). Since phosphate (Pi) control and iron replacement are common therapies in CKD, the aim of our study was to investigate the effect of iron on high Pi-induced VC in rat vascular smooth muscle cells (VSMCs). METHODS: We treated VSMCs with 5 mM Pi and iron citrate (Fe(3+)) to evaluate Ca deposition by Alizarin Red destaining, DNA fragmentation by ELISA, gene expression by RT-PCR and protein expression by Western blot...
September 30, 2016: Atherosclerosis
Thomas W Bastian, William C von Hohenberg, Daniel J Mickelson, Lorene M Lanier, Michael K Georgieff
Iron deficiency (ID), with and without anemia, affects an estimated 2 billion people worldwide. ID is particularly deleterious during early-life brain development, leading to long-term neurological impairments including deficits in hippocampus-mediated learning and memory. Neonatal rats with fetal/neonatal ID anemia (IDA) have shorter hippocampal CA1 apical dendrites with disorganized branching. ID-induced dendritic structural abnormalities persist into adulthood despite normalization of the iron status. However, the specific developmental effects of neuronal iron loss on hippocampal neuron dendrite growth and branching are unknown...
2016: Developmental Neuroscience
Sabine Klein, Christian Hinüber, Kanishka Hittatiya, Robert Schierwagen, Frank Erhard Uschner, Christian P Strassburg, Hans-Peter Fischer, Ulrich Spengler, Jonel Trebicka
BACKGROUND: Non-cirrhotic idiopathic portal hypertension (NCIPH) is characterized by splenomegaly, anemia and portal hypertension, while liver function is preserved. However, no animal models have been established yet. This study assessed a rat model of NCIPH and characterized the hemodynamics, and compared it to human NCIPH. METHODS: Portal pressure (PP) was measured invasively and coloured microspheres were injected in the ileocecal vein in rats. This procedure was performed weekly for 3 weeks (weekly embolization)...
2016: PloS One
N N Lasheen, G F Mohamed
Metabolic acidosis could occur due to either endogenous acids accumulation or bicarbonate loss from the gastrointestinal tract or commonly from the kidney. This study aimed to investigate the possible underlying mechanism(s) of chronic acidosis-induced cardiac contractile and electrical changes in rats. Twenty four adult Wistar rats, of both sexes, were randomly divided into control group and chronic metabolic acidosis group, which received orally 0.28 M NH(4)Cl in the drinking water for 2 weeks. At the end of experimental period, systolic and diastolic blood pressure values were measured...
August 19, 2016: Physiological Research
Chih-Wei Chang, Yi-Ming Chen, Yi-Ju Hsu, Chi-Chang Huang, Yu-Tse Wu, Mei-Chich Hsu
ETHNOPHARMACOLOGICAL RELEVANCE: Sports anemia is a persistent and severe problem in athletes owing to strenuous exercise-induced oxidative stress and hepcidin upregulation. The roots of Angelica sinensis (AS), a familiar traditional Chinese medicine, has been used for replenishing blood since antiquity. AIM OF THE STUDY: To evaluate the effects of ethanolic AS extract in a 4-week study on sports anemia in female Wistar rats. MATERIALS AND METHODS: To induce anemia, a strenuous exercise protocol consisting of running and swimming was employed with increasing intensity...
August 3, 2016: Journal of Ethnopharmacology
N V Tishevskaya, A A Bolotov, Ya E Lebedeva
In 14 days after triple subcutaneous injection of benzene to rats in a dose of 0.05 ml/100 g body weight, the number of erythroblastic islands in the bone marrow 2-fold decreased, de novo erythropoiesis ceased, de repeto erythropoiesis slowed down, and the number of lymphoid cells contacting with erythroblastic islands decreased. By day 56 of the experiment, proliferation of erythroid cells in the bone marrow start to recover due to erythropoiesis reconstitution, but the number of reticulocytes in the blood remained low...
July 2016: Bulletin of Experimental Biology and Medicine
A O Ige, E O Adewoye
Magnesium has been reported to improve glucose utilization in diabetes mellitus. However, information on its effects on anemic and inflammatory markers in diabetes mellitus is limited. This study investigated the effect of oral magnesium (Mg) treatment on some markers of anemia and inflammation in 25 male Wistar rats. Rats (200 ± 15 g) were randomly divided into five groups (n = 5). Group 1 was control (received orally 0.2 mL distilled water daily), group 2 (Diabetic Untreated), group 3 (Diabetic Mg treated-100 mg/kg bw), group 4 (Diabetic Mg treated-250 mg/kg bw), group 5 (Diabetic Insulin treated-1 IU/kg bw)...
July 26, 2016: Journal of Dietary Supplements
Xue-Lian Gong, Xiao-Lei Gu, Yong-Chun Chen, Hai Zhu, Zhen-Na Xia, Jian-Zhong Li, Guo-Cai Lu
EPO-018B, a synthetic peptide-based erythropoiesis stimulating agent (ESA), is mainly designed for treatment of anemia caused by chronic renal failure and chemotherapy against cancer. It overcomes the deficiencies of currently approved ESA, including the frequent administration of temperature-sensitive recombinant protein and anti-EPO antibody-mediated pure red cell aplasia (PRCA). This study was designed to evaluate the potential chronic toxicity of EPO-018B. Subcutaneous administration doses were designed as 0, 0...
September 15, 2016: Toxicology and Applied Pharmacology
Michael Winter, Jürgen Funk, Annette Körner, Daniela Alberati, Francois Christen, Georg Schmitt, Bernd Altmann, Andreas Pospischil, Thomas Singer
Glycine is a key rate-limiting component of heme biosynthesis in erythropoietic cells, where the high intracellular glycine demand is primarily supplied by the glycine transporter 1 (GlyT1). The impact of intracellular glycine restriction after GlyT1 inhibition on hematopoiesis and iron regulation is not well established. We investigated the effects of a potent and selective inhibitor of GlyT1, bitopertin, on erythropoiesis and iron homeostasis in rats. GlyT1 inhibition significantly affected erythroid heme biosynthesis, manifesting as microcytic hypochromic regenerative anemia with a 20% steady-state reduction in hemoglobin...
October 2016: Experimental Hematology
Ines G Alamo, Kolenkode B Kannan, Michael A Smith, Philip A Efron, Alicia M Mohr
BACKGROUND: The cause of persistent injury-associated anemia is multifactorial and includes acute blood loss, an altered erythropoietin (EPO) response, dysregulation of iron homeostasis, and impaired erythropoiesis in the setting of chronic inflammation/stress. Hepcidin plays a key role in iron homeostasis and is regulated by anemia and inflammation. Erythropoietin is a main regulator of erythropoiesis induced by hypoxia. A unique rodent model of combined lung injury (LC)/hemorrhagic shock (HS) (LCHS)/chronic restraint stress (CS) was used to produce persistent injury-associated anemia to further investigate the roles of EPO, hepcidin, iron, ferritin, and the expression of EPO receptors (EPOr)...
October 2016: Journal of Trauma and Acute Care Surgery
Valeria A Kostevich, Alexey V Sokolov, Stanislav O Kozlov, Anna Yu Vlasenko, Nikolay N Kolmakov, Elena T Zakharova, Vadim B Vasilyev
Strongly pronounced argyrosis caused by adding AgCl to the feed of laboratory rats efficiently mimics the deficiency of ceruloplasmin (CP) ferroxidase activity. Bringing the concentration of AgCl in the feedstuff of lactating rats to 250 mg % and keeping their progeny (Ag-rats) for 3 months on the same silver-containing feed provided the serum iron content 1.4 times lower than that in the control group. Besides, the ferroxidase activity of CP dropped to zero. In CP purified from sera of Ag-rats two copper ions were substituted with two silver ions...
August 2016: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Marina M Hanson, Fengming Liu, Shen Dai, Alison Kearns, Xuebin Qin, Elizabeth C Bryda
Effective methods for cell ablation are important tools for examining the anatomical, functional, and behavioral consequences of selective loss of specific cell types in animal models. We have developed an ablation system based on creating genetically modified animals that express human CD59 (hCD59), a membrane receptor, and administering intermedilysin (ILY), a toxin produced by Streptococcus intermedius, which binds specifically to hCD59 to induce cell lysis. As proof-of-concept in the rat, we generated an anemia model, SD-Tg(CD59-HBA1)Bryd, which expresses hCD59 on erythrocytes...
August 1, 2016: Physiological Genomics
Murui Han, JuOae Chang, Jonghan Kim
The divalent metal transporter 1 (DMT1) is a major iron transporter required for iron absorption and erythropoiesis. Loss of DMT1 function results in microcytic anemia. While iron plays an important role in neural function, the behavioral consequences of DMT1 deficiency are largely unexplored. The goal of this study was to define the neurobehavioral and neurochemical phenotypes of homozygous Belgrade (b/b) rats that carry DMT1 mutation and explore potential mechanisms of these phenotypes. The b/b rats (11-12 weeks old) and their healthy littermate heterozygous (+/b) Belgrade rats were subject to elevated plus maze tasks...
September 2016: Journal of Neurochemistry
Mauricio Martínez-Zavala, María Alejandra Mora-Avilés, Miriam Aracely Anaya-Loyola, Horacio Guzmán-Maldonado, Araceli Aguilera-Barreyro, Alejandro Blanco-Labra, Teresa García-Gasca
Recent findings made by our group indicate that the iron content in Phaseolus vulgaris leaves is at least four times greater than in grains therefore, we evaluated the effect of supplementation with bean leaf (iron content of 275 mg/kg on a dry basis) in iron-deficient rats. Anemia was induced by feeding rats with an iron-deficient diet (IDD) for 11 days and iron-recovery diets were subsequently tested for 14 days using a normal diet, a 10 % bean leaf-supplemented IDD (BLSD) or a ferrous sulfate-supplemented IDD...
September 2016: Plant Foods for Human Nutrition
Masakazu Saitoh, Michiyoshi Hatanaka, Masaaki Konishi, Junichi Ishida, Sandra Palus, Nicole Ebner, Wolfram Döhner, Stephan von Haehling, Stefan D Anker, Jochen Springer
BACKGROUND: Erythropoietin administration, which is clinically used in cancer patients with cancer-induced anemia, has also potentially beneficial effects on nonhematopoietic organs. We assessed the effects of erythropoietin on cancer cachexia progression and cardiac wasting compared with placebo using the Yoshida hepatoma model. METHODS: Wistar rats were divided in a sham group (n=10) and a tumor-bearing group (n=60). The tumor-bearing group was further randomized to placebo (n=28), 500Unit/kg/day (n=16) or 5000Unit/kg/day of erythropoietin (n=16)...
September 1, 2016: International Journal of Cardiology
Xiaona Hu, Ranran Wang, Zhongyan Shan, Yujie Dong, Hongzhi Zheng, Forrest Fabian Jesse, Elizabeth Rao, Eiki Takahashi, Weidong Li, Weiping Teng, Xiaochun Teng
BACKGROUND: Both perinatal hypothyroxinemia and perinatal iron deficiency (ID) are associated with poor neurodevelopment in offspring. Iron is an important component of thyroid peroxidase, a key enzyme in the synthesis of thyroid hormone. The authors' previous study demonstrated that perinatal ID can lead to maternal hypothyroxinemia during pregnancy. The goal of this study was to determine whether perinatal ID-associated hypothyroxinemia can cause brain defects prior to neonatal brain iron depletion...
July 2016: Thyroid: Official Journal of the American Thyroid Association
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