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Burcu Bayyurt, Serdal Arslan, Aynur Engin, Mehmet Bakir
Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease caused by the Crimean-Congo hemorrhagic fever virus (CCHFV). Long non-coding RNAs (lncRNAs) are generally classified as transcripts longer than 200 nucleotides (nt). The various lncRNAs expressed in infected cells are responsible for regulating the expression of viral and host genes. This is the first study to investigate hepatocellular carcinoma up-regulated long non-coding RNA (HULC) and 7SL RNA expression levels in CCHF patients. Blood samples were taken from 100 individuals (60 patients and 40 controls) and total RNA isolation was performed...
July 17, 2018: Journal of Medical Virology
Hilary E Baldwin, Linda F Stein Gold, Kenneth B Gordon, Jeremy B Green, Craig L Leonardi, Roberta D Sengelmann
Updates on managing some of the most common dermatologic conditions for which patients seek care illuminated presentations at the Skin Disease Education Foundation's 42nd Annual Hawaii Dermatology Seminar®. This educational supplement summarizes the highlights of clinical sessions presented during this CME/CE conference. Treatment of psoriasis has continued to advance, with three interleukin (IL)-17 antagonists approved by the US Food and Drug Administration (FDA) and a fourth in phase 3 trials. An authority on the use of biologics in psoriasis presents current data on the safety and efficacy of these therapies...
June 2018: Seminars in Cutaneous Medicine and Surgery
Michelle J Schmahl, Daniel P Regan, Adam C Rivers, William C Joesten, Michael A Kennedy
Pancreatic cancer is the third leading cause of cancer deaths in the United States with more than 53,000 expected to be diagnosed with the disease in 2018. The median survival time after diagnosis is four to six months. The poor survival statistics are due in part to the fact that pancreatic cancer is typically asymptomatic until it reaches advanced stages of the disease. Although surgical resection provides the best chance of survival, pancreatic cancer is rarely detected when surgery is still possible due, in part, to lack of effective biomarkers for early detection...
2018: PloS One
José L Mansura
The main source of vitamin D is produced by the exposure of sunlight on the skin. It has been proven that the deficit is frequent and also occurs in pregnant women. Possible consequences may be preeclampsia and others. The level of vitamin D in breast milk depends on the level of the mother, so that deficient mothers produce milk with low concentration. Possible consequences for the baby are rickets and seizures, but also lower respiratory tract infections or risk of type 1 diabetes. Various suggestions have been published...
August 1, 2018: Archivos Argentinos de Pediatría
Jing Li, Zhongjun Ding, Yue Yang, Baohong Mao, Yanxia Wang, Xiaoying Xu
Pregnancy complications are associated with abnormal cytotrophoblast differentiation and invasion. Hydrogen peroxide (H2O2) is an important mediator of oxidative ischemia/reperfusion stress in the placenta. Lycium barbarum polysaccharides (LBP) have been demonstrated to counteract oxidative free radicals. The effects of LBP in trophoblast HTR8/SVneo cells injured with H2O2 were examined. A cell counting kit‑8 assay was performed to detect the effect of LBP at different concentrations on the proliferative ability of H2O2 injured trophoblast cells...
July 12, 2018: Molecular Medicine Reports
Li Fan, Chunxian Huang, Jing Li, Tian Gao, Zhongqiu Lin, Tingting Yao
Cervical cancer is one of the most common types of female malignant tumor. It is well established that radiotherapy (RT) is the first‑line treatment of cervical cancer; however, radioresistance is a substantial obstacle to cervical cancer RT. At present, the mechanism underlying radioresistance remains unclear. Emerging evidence has demonstrated that long non‑coding RNAs (lncRNAs) function as crucial regulators of diverse cancers. Aerobic glycolysis, which is a common phenomenon in cancer cells, is associated with various biological functions, including radioresistance...
July 13, 2018: International Journal of Molecular Medicine
Dong Wang, Xinjie Zhang, Daoxu Qu, Jichun Han, Fanqing Meng, Minglei Xu, Qiusheng Zheng
The present study used an in vitro model of cold cardioplegia in isolated working rat hearts to evaluate the possible effects of two flavonoids, astragalin and dihydromyricetin, as adjuncts to histidine‑tryptophan‑ketoglutarate (HTK) cardioplegia. The following three groups of male Sprague Dawley rats were evaluated: The HTK group, treated with HTK alone; the HTK‑A group, treated with 10 µmol/l astragalin; and the HTK‑D group, treated with 10 µmol/l dihydromyricetin. Isolated rat hearts were perfused with Krebs‑Henseleit buffer for 30 min and incubated with the respective cardioplegic solution for 6 h at 4˚C...
July 5, 2018: Molecular Medicine Reports
Silvia Jiménez-Morales, Carlos J Pérez-Amado, Elizabeth Langley, Alfredo Hidalgo-Miranda
High lactate production in cells during growth under oxygen-rich conditions (aerobic glycolysis) is a hallmark of tumor cells, indicating the role of mitochondrial function in tumorigenesis. In fact, enhanced mitochondrial biogenesis and impaired quality control are frequently observed in cancer cells. Mitochondrial DNA (mtDNA) encodes 13 subunits of oxidative phosphorylation (OXPHOS), is present in thousands of copies per cell, and has a very high mutation rate. Mutations in mtDNA and nuclear DNA (nDNA) genes encoding proteins that are important players in mitochondrial biogenesis and function are involved in oncogenic processes...
July 4, 2018: International Journal of Oncology
Shihua Zhou, Ziqian Min, Kang Sun, Sijie Qu, Jinrun Zhou, Hongyan Duan, Huawei Liu, Xiaowen Liu, Zhijun Gong, Dan Li
Aerobic glycolysis is one of the characteristics of tumor metabolism and contributes to the development of tumors. Studies have identified that microRNA (miRNA/miR) serves an important role in glucose metabolism of tumors. miR‑199a‑3p is a member of the miR‑199a family that controls the outcomes of cell survival and death processes, and previous studies have indicated that the expression of miR‑199a‑3p is low and may be an inhibitor in several cancer types, including testicular tumors. The present study discussed the role and underlying mechanism of miR‑199a‑3p in aerobic glycolysis of Ntera‑2 cells and identified its downstream factors...
July 12, 2018: International Journal of Molecular Medicine
Zhengyi He, Lei Jiang, Ting Zhang, Minya Zhou, Daijin Wu, Tingting Yuan, Yuguo Yuan, Yong Cheng
Expression efficacy of recombinant protein in current expression systems is generally low. Therefore, the expression levels of recombinant proteins in the breast milk of transgenic animals are typically low. In view of this, the present study aimed to construct homozygous transgenic rabbits with a high expression level of recombinant human plasminogen activator (rhPA) during the entire lactation period. Homozygous transgenic rabbits were obtained using an effective rhPA mammary‑specific expression vector PCL25/rhPA...
July 4, 2018: International Journal of Molecular Medicine
Chanu Rhee, Michael R Filbin, Anthony F Massaro, Amy L Bulger, Donna McEachern, Kathleen A Tobin, Barrett T Kitch, Bert Thurlo-Walsh, Aran Kadar, Alexandra Koffman, Anupam Pande, Yasir Hamad, David K Warren, Travis M Jones, Cara O'Brien, Deverick J Anderson, Rui Wang, Michael Klompas
OBJECTIVES: Many septic patients receive care that fails the Centers for Medicare and Medicaid Services' SEP-1 measure, but it is unclear whether this reflects meaningful lapses in care, differences in clinical characteristics, or excessive rigidity of the "all-or-nothing" measure. We compared outcomes in cases that passed versus failed SEP-1 during the first 2 years after the measure was implemented. DESIGN: Retrospective cohort study. SETTING: Seven U...
June 28, 2018: Critical Care Medicine
Patrik Gilje, Martin Frydland, John Bro-Jeppesen, Josef Dankiewicz, Hans Friberg, Malin Rundgren, Yvan Devaux, Pascal Stammet, Mariam Al-Mashat, Jonas Jögi, Jesper Kjaergaard, Christian Hassager, David Erlinge
BACKGROUND: Data suggests that the plasma levels of the liver-specific miR-122-5p might both be a marker of cardiogenic shock and a prognostic marker of out-of-hospital cardiac arrest (OHCA). Our aim was to characterize plasma miR-122-5p at admission after OHCA and to assess the association between miR-122-5p and relevant clinical factors such all-cause mortality and shock at admission after OHCA. METHODS: In the pilot trial, 10 survivors after OHCA were compared to 10 age- and sex-matched controls...
July 17, 2018: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Daniel A Morgenstern, Ulrike Pötschger, Lucas Moreno, Vassilios Papadakis, Cormac Owens, Shifra Ash, Claudia Pasqualini, Roberto Luksch, Alberto Garaventa, Adela Canete, Martin Elliot, Aleksandra Wieczorek, Geneviève Laureys, Per Kogner, Josef Malis, Ellen Ruud, Maja Beck-Popovic, Gudrun Schleiermacher, Dominique Valteau-Couanet, Ruth Ladenstein
BACKGROUND: Risk stratification is crucial to treatment decision-making in neuroblastoma. This study aimed to explore factors present at diagnosis affecting outcome in patients aged ≥18 months with metastatic neuroblastoma and to develop a simple risk score for prognostication. PROCEDURE: Data were derived from the European high-risk neuroblastoma 1 (HR-NBL1)/International Society for Paediatric Oncology European Neuroblastoma (SIOPEN) trial with analysis restricted to patients aged ≥18 months with metastatic disease and treated prior to the introduction of immunotherapy...
July 17, 2018: Pediatric Blood & Cancer
J J van Wattum, T M Leferink, B Wilffert, P G J Ter Horst
Breastfeeding is important for the development of the child. Many antibiotics are considered safe during breastfeeding. The aim of the study was to assess the quality of lactation studies with antibiotics using the FDA and International Lacation Consultant Association (ILCA) quality guidelines for lactation studies. The secondary goal was to determine the exposure of the breastfed infant to antibiotics in relation to bacterial resistance and the developing microbiome. A literature search was performed and the included studies were scored on methodology, parameters concerning maternal exposure to antibiotics, maternal plasma and milk sampling...
July 17, 2018: Basic & Clinical Pharmacology & Toxicology
Kyung Mi Yang, Kunhong Kim
Protein kinase CK2 is active in cancer cells. Previously, we reported that increased CK2 activity could induce epithelial mesenchymal transition of cancer cells. CK2 also induced epithelial mesenchymal transition in colon cancer cell lines such as HT29 and SW620, and the transitioned cells (CK2α cells) became more proliferative than the controls. We assumed that CK2 could affect cancer cell growth by modulating their energy metabolism. Here, we examined the molecular effects of CK2 on the glucose metabolism of cancer cells...
July 17, 2018: Journal of Cellular Biochemistry
Maximilian Kreibich, Georg Trummer, Friedhelm Beyersdorf, Christian Scherer, Katharina Förster, Itumeleng Taunyane, Christoph Benk
The reperfusion period after extracorporeal cardiopulmonary resuscitation has been recognized as a key player in improving the outcome after cardiac arrest (CA). Our aim was to evaluate the effects of high mean arterial pressure (MAP) and pulsatile flow during controlled automated reperfusion of the whole body. Following 20 min of normothermic CA, high MAP, and pulsatile blood flow (pulsatile group, n = 10) or low MAP and nonpulsatile flow (nonpulsatile group, n = 6) controlled automated reperfusion of the whole body was commenced through the femoral vessels of German landrace pigs for 60 min...
July 17, 2018: Artificial Organs
Shiue-Lin Li, Jui-Hung Yen, Kenji Kano, Shiu-Mei Liu, Chien-Liang Liu, Sheng-Shung Cheng, Hsun-Yi Chen
Using an electrochemical cell equipped with carbon felt electrodes (poised at +0.63 V vs. SHE), the current production capabilities of two Shewanella strains-NTOU1 and KR-12-were examined under various conditions with lactate as an electron donor. The metabolic charge produced in the tricarboxylic acid cycle (QTCA ) was calculated by mass-balance. The data showed a linear relation between the electric coulomb production (QEL ) and QTCA with an R2 of 0.65. In addition, a large amount of pyruvate accumulation was observed at pH = 6, rendering QTCA negative...
July 6, 2018: Bioelectrochemistry
Sachin Karkale, Amit Khurana, Mohd Aslam Saifi, Chandraiah Godugu, Venu Talla
The purpose of this study was to investigate the protective effect of andrographolide in silica-induced pulmonary fibrosis (PF) in mice and its underlying mechanisms. Male Swiss albino mice were divided into five groups: Normal control group, disease control group (1.5 mg silica/60 μL/mice) via oropharyngeal route, low dose (LD) group received silica + andrographolide (3 mg/kg), high dose (HD) group received silica + andrographolide (10 mg/kg), andrographolide per se group received 10 mg/kg andrographolide...
July 13, 2018: International Immunopharmacology
Robert P Hausinger, Benoît Desguin, Matthias Fellner, Joel A Rankin, Jian Hu
A novel organometallic cofactor, nickel pyridinium-3,5-dithiocarboxylic acid mononucleotide, was recently discovered in lactate racemase (LarA) of Lactobacillus plantarum. This review summarizes the substantial progress made in uncovering the function of this cofactor as a transient hydride acceptor in the LarA mechanism. The latest developments related to cofactor biosynthesis reveal insights into a pathway in which LarB serves as a nicotinic acid adenine dinucleotide hydrolase/carboxylase, LarE acts as a sacrificial sulfur transferase, and LarC functions as a nickel insertase, forming the nickel-pincer nucleotide cofactor that becomes covalently tethered to LarA in some bacteria...
July 9, 2018: Current Opinion in Chemical Biology
Takashi Temma, Hidekazu Kawashima, Naoya Kondo, Makoto Yamazaki, Kazuhiro Koshino, Hidehiro Iida
INTRODUCTION: Lactate could serve as an energy source and signaling molecule in the brain, although there is insufficient in vivo evidence to support this possibility. Here we aimed to use a one-pot enzymatic synthetic procedure to synthesize l-[3-11 C]lactate that can be used to evaluate chemical forms in the blood after intravenous administration, and as a probe for pharmacokinetic analysis of lactate metabolism in in vivo positron emission tomography (PET) scans with normal and fasted rats...
July 7, 2018: Nuclear Medicine and Biology
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