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Gene expression in peripheral blood cells in response to oxidative stress

Akira Torigoe, Emiko Sato, Takefumi Mori, Norio Ieiri, Chika Takahashi, Yoko Ishida, Osamu Hotta, Sadayoshi Ito
Introduction Oxidative stress is one of the main mediators of progression of chronic kidney diseases (CKD). Nuclear factor E2-related factor 2 (Nrf2) is the transcription factor of antioxidant and detoxifying enzymes and related proteins which play an important role in cellular defense. Long-time hemodialysis (HD) therapy (8 hours) has been considered to be more beneficial compared to normal HD therapy (4 hours). We investigated oxidative response related to Nrf2 in peripheral blood mononuclear cells (PBMCs) of long-time HD and normal HD patients...
October 2016: Hemodialysis International
Sheeja Liza Easo, Parayanthala Valappil Mohanan
Iron oxide nanoparticles present an attractive choice for carcinogenic cell destruction via hyperthermia treatment due to its small size and magnetic susceptibility. Dextran stabilized iron oxide nanoparticles (DIONPs) synthesized and characterized for this purpose were used to evaluate its effect on cellular uptake, cytotoxicity, and oxidative stress response in human peripheral blood lymphocytes. In the absence of efficient internalization and perceptible apoptosis, DIONPs were still capable of inducing significant levels of reactive oxygen species formation shortly after exposure...
2016: Biointerphases
Anna Maria Fratta Pasini, Marcello Ferrari, Chiara Stranieri, Paola Vallerio, Chiara Mozzini, Ulisse Garbin, Giorgia Zambon, Luciano Cominacini
Inadequacy of antioxidant nuclear factor-E2-related factor 2 (Nrf2) and endoplasmic reticulum stress-mediated unfolded protein response has been implicated in severe chronic obstructive pulmonary disease (COPD) and cigarette smoking-induced emphysema. As evidence suggests that the ability to upregulate Nrf2 expression may influence the progression of COPD and no data exist up to now in ex-smokers with mild-moderate COPD, this study was first aimed to evaluate Nrf2 and unfolded protein response expression in peripheral blood mononuclear cells (PBMC) of mild-moderate ex-smokers with COPD compared to smoking habit-matched non-COPD subjects...
2016: International Journal of Chronic Obstructive Pulmonary Disease
Behrooz Soltani, Nasser Ghaemi, Majid Sadeghizadeh, Farhood Najafi
Exposure to ionizing radiation (IR) could be detrimental to health. Oxidative stress, DNA damage, and inflammation are implicated in radiation damage. Curcumin, a natural polyphenol, has remarkable antioxidant, anti-inflammation and anticarcinogenic properties and is reported to protect cells and organisms against gamma-rays. We have recently enhanced solubility of curcumin via a novel dendrosomal nanoformulation (DNC). The objective of this study was to assess the potential efficacy of this nanoformulation in protecting human peripheral blood mononuclear cells (PBMC) against gamma-radiation...
September 25, 2016: Chemico-biological Interactions
Jusciele B Moreli, Janine H Santos, Aline Rodrigues Lorenzon-Ojea, Simone Corrêa-Silva, Rodrigo S Fortunato, Clarissa Ribeiro Rocha, Marilza V Rudge, Débora C Damasceno, Estela Bevilacqua, Iracema M Calderon
OBJECTIVE: Investigate the DNA damage and its cellular response in blood samples from both mother and the umbilical cord of pregnancies complicated by hyperglycemia. METHODS: A total of 144 subjects were divided into 4 groups: normoglycemia (ND; 46 cases), mild gestational hyperglycemia (MGH; 30 cases), gestational diabetes mellitus (GDM; 45 cases) and type-2 diabetes mellitus (DM2; 23 cases). Peripheral blood mononuclear cell (PBMC) isolation and/or leukocytes from whole maternal and umbilical cord blood were obtained from all groups at delivery...
2016: International Journal of Biological Sciences
Ebun Omoyinmi, Raja Hamaoui, Annette Bryant, Mike Chao Jiang, Trin Athigapanich, Despina Eleftheriou, Mike Hubank, Paul Brogan, Patricia Woo
BACKGROUND: Various pathways involved in the pathogenesis of sJIA have been identified through gene expression profiling in peripheral blood mononuclear cells (PBMC), but not in neutrophils. Since neutrophils are important in tissue damage during inflammation, and are elevated as part of the acute phase response, we hypothesised that neutrophil pathways could also be important in the pathogenesis of sJIA. We therefore studied the gene profile in both PBMC and neutrophils of sJIA patients treated with tocilizumab...
2016: Pediatric Rheumatology Online Journal
Mark L Laudenslager, Teresa L Simoneau, Sam Philips, Patrick Benitez, Crystal Natvig, Steve Cole
Few studies have addressed whether stress-associated physiological changes in caregivers are reversible by psychological interventions mitigating distress. We report on pro-inflammatory, sympathetic, and oxidative stress gene expression in response to stress management for caregivers of allogeneic hematopoietic stem cell transplant (Allo-HSCT) patients. Following randomization by permuted block to either treatment as usual (TAU, n = 11) or a stress management intervention (PsychoEducation, Paced Respiration, and Relaxation, PEPRR, n = 13), twenty-four caregivers were selected at the conclusion of a larger trial of 149 caregivers...
April 2016: Journal of Behavioral Medicine
Jacqueline S Garcia, Min Huang, Bruno C Medeiros, Beverly S Mitchell
PURPOSE: This study was performed to determine whether the investigational proteasome inhibitor ixazomib demonstrated selective antineoplastic activity against acute myelogenous leukemia cells expressing a mutated nucleophosmin-1 gene and to gain a better understanding of its mechanisms of action. EXPERIMENTAL DESIGN: The cytotoxic effects of ixazomib treatment were analyzed in human acute myelogenous leukemia (AML) cell lines and primary AML samples expressing wild-type or mutated NPM1 (NPMc(+))...
April 15, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Sara Mina, Cindy Staerck, Sènan M d'Almeida, Agnès Marot, Yves Delneste, Alphonse Calenda, Julie Tabiasco, Jean-Philippe Bouchara, Maxime J J Fleury
Scedosporium boydii is an opportunistic filamentous fungus which may be responsible for a large variety of infections in both immunocompetent and immunocompromised individuals. This fungus belongs to the Scedosporium apiospermum species complex which usually ranks second among the filamentous fungi colonizing the airways of patients with cystic fibrosis (CF). Species of the S. apiospermum complex are able to chronically colonize the CF airways suggesting pathogenic mechanisms allowing persistence and growth of these fungi in the respiratory tract...
December 2015: Fungal Biology
Tineke C T M van der Pouw Kraan, Weena J Chen, Mathijs C M Bunck, Daniel H van Raalte, Nynke J van der Zijl, Renate E van Genugten, Liselotte van Bloemendaal, Josefien M Baggen, Erik H Serné, Michaela Diamant, Anton J G Horrevoets
BACKGROUND: Metabolic syndrome (MetS) is characterized by central obesity, insulin resistance, dysglycemia, and a pro-atherogenic plasma lipid profile. MetS creates a high risk for development of type 2 diabetes (T2DM) and cardiovascular disease (CVD), presumably by altering inflammatory responses. Presently, it is unknown how the chronic metabolic disturbances in acute hyperglycemia, MetS and T2DM affect the immune activity of peripheral blood cells. METHODS: We performed genome-wide expression analysis of peripheral blood cells obtained from patients with T2DM (n = 6) and age-, sex- , BMI- and blood pressure-matched obese individuals with MetS (n = 4) and lean healthy normoglycemic controls (n = 3), both under fasting conditions and after controlled induction of acute hyperglycemia during a 70 min hyperglycemic clamp...
2015: BMC Medical Genomics
Braden Kuo, Manoj Bhasin, Jolene Jacquart, Matthew A Scult, Lauren Slipp, Eric Isaac Kagan Riklin, Veronique Lepoutre, Nicole Comosa, Beth-Ann Norton, Allison Dassatti, Jessica Rosenblum, Andrea H Thurler, Brian C Surjanhata, Nicole N Hasheminejad, Leslee Kagan, Ellen Slawsby, Sowmya R Rao, Eric A Macklin, Gregory L Fricchione, Herbert Benson, Towia A Libermann, Joshua Korzenik, John W Denninger
INTRODUCTION: Irritable Bowel Syndrome (IBS) and Inflammatory Bowel Disease (IBD) can profoundly affect quality of life and are influenced by stress and resiliency. The impact of mind-body interventions (MBIs) on IBS and IBD patients has not previously been examined. METHODS: Nineteen IBS and 29 IBD patients were enrolled in a 9-week relaxation response based mind-body group intervention (RR-MBI), focusing on elicitation of the RR and cognitive skill building. Symptom questionnaires and inflammatory markers were assessed pre- and post-intervention, and at short-term follow-up...
2015: PloS One
Jette Gjerke Hemmingsen, Kim Jantzen, Peter Møller, Steffen Loft
Exposure to traffic-related particulate matter (PM) has been associated with increased risk of lung disease, cancer and cardiovascular disease especially in elderly and overweight subjects. The proposed mechanisms involve intracellular production of reactive oxygen species (ROS), inflammation and oxidation-induced DNA damage studied mainly in young normal-weight subjects. We performed a controlled cross-over, randomised, single-blinded, repeated-measure study where 60 healthy subjects (25 males and 35 females) with age 55-83 years and body mass index above 25 kg/m(2) were exposed for 5h to either particle-filtered or sham-filtered air from a busy street with number of concentrations and PM2...
September 2015: Mutagenesis
J Talvas, G Garrait, N Goncalves-Mendes, J Rouanet, J Vergnaud-Gauduchon, F Kwiatkowski, P Bachmann, C Bouteloup, J Bienvenu, M-P Vasson
BACKGROUND: Immunonutrition has been reported to improve the immune status of perioperative cancer patients, thereby reducing complications and length of hospital stay. AIM: This study aimed to assess whether immunonutrition enriched in arginine, EPA & DHA and nucleotides could impact the immune cells responses in head & neck and esophageal cancer patients treated by radiochemotherapy (RCT). METHODS: A double-blind clinical trial was carried out in 28 patients randomized into two groups, receiving either an immunomodulating enteral nutrition formula (IEN, n = 13, Impact(®), Nestlé) or an isoenergetic isonitrogenous standard enteral nutrition formula (SEN, n = 15) throughout RCT (5-7 weeks)...
October 2015: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
Eve Ramery, Audrey Fraipont, Eric A Richard, Tatiana Art, Dimitri Pirottin, Wouter van Delm, Fabrice Bureau, Pierre Lekeux
BACKGROUND: Inflammatory airway disease (IAD) affects performance and well-being of horses. Diagnosis is primarily reached by bronchoalveolar lavage (BAL) cytology which is invasive and requires sedation. OBJECTIVES: The purpose of this study was to identify differential gene expression in peripheral blood of horses with IAD using species-specific expression microarrays. METHODS: Equine gene expression microarrays were used to investigate global mRNA expression in circulating leukocytes from healthy, IAD-affected, and low-performing Standardbred and endurance horses...
March 2015: Veterinary Clinical Pathology
Ruiwei Jiang, Meaghan J Jones, Francesco Sava, Michael S Kobor, Christopher Carlsten
BACKGROUND: Changes in DNA methylation have been associated with traffic-related air pollution in observational studies, but the specific mechanisms and temporal dynamics therein have not been explored in a controlled study of asthmatics. In this study, we investigate short-term effects of diesel exhaust inhalation on DNA methylation levels at CpG sites across the genome in circulating blood in asthmatics. METHODS: A double-blind crossover study of filtered air and diesel exhaust exposures was performed on sixteen non-smoking asthmatic subjects...
2014: Particle and Fibre Toxicology
Alexandra E Turley, Joseph W Zagorski, Cheryl E Rockwell
The transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) regulates a battery of antioxidant, detoxification, and cell stress genes. It is activated by oxidative stress and a number of exogenous compounds, one of which is tert-butylhydroquinone (tBHQ), a widely used food preservative. Nrf2 modulates immune responses in numerous rodent models of inflammation, but its effects on human immune cells are not well characterized. The purpose of these studies was to evaluate the effects of the Nrf2 activator tBHQ on early events of T cell activation in primary human cells...
February 2015: Cytokine
Hyun-Kyung Na, Minju Kim, Seong-Sil Chang, Soo-Young Kim, Jong Y Park, Myeon Woo Chung, Mihi Yang
Tobacco smoking is a well-known cause of various diseases, however, its toxic mechanisms for diseases are not completely understood, yet. Therefore, we performed biological monitoring to find tobacco smoking-responsive mechanisms including oxidative stress in Korean men (N=36). Whole genome microarray analyses were performed with peripheral blood from smokers and age-matched nonsmokers. We also performed qRT-PCR to confirm the microarray results and compared the gene expression of blood to those of buccal cells...
January 22, 2015: Toxicology Letters
Stine M Ulven, Mari C Myhrstad, Kirsten B Holven
Intake of marine n-3 fatty acids has been shown to have beneficial effects on cardiovascular disease. Gene expression analyses in peripheral blood mononuclear cells (PBMCs) are used to understand the underlying mechanisms of action of marine n-3 fatty acids. The aim of this review was to summarize the effects mediated by marine n-3 fatty acids on gene expression in PBMCs. A systematic literature search was conducted in PubMed in May 2014 and 14 papers were included. Targeted gene expression studies were reported in 9 papers and focused on genes involved in lipid metabolism and inflammation...
2014: Current Cardiovascular Risk Reports
Haytham Ali, Omima Galal, Yoshishige Urata, Shinji Goto, Chang-Ying Guo, Lan Luo, Eman Abdelrahim, Yusuke Ono, Emtethal Mostafa, Tao-Sheng Li
Nicaraven, a hydroxyl radical-specific scavenger has been demonstrated to attenuate radiation injury in hematopoietic stem cells with 5Gy γ-ray exposures. We explored the effect and related mechanisms of nicaraven for protecting radiation injury induced by sequential exposures to a relatively lower dose γ-ray. C57BL/6 mice were given nicaraven or placebo within 30min before exposure to 50mGy γ-ray daily for 30days in sequences (cumulative dose of 1.5Gy). Mice were victimized 24h after the last radiation exposure, and the number, function and oxidative stress of hematopoietic stem cells were quantitatively estimated...
September 26, 2014: Biochemical and Biophysical Research Communications
Dariusz Suchy, Krzysztof Łabuzek, Łukasz Bułdak, Dawid Szkudłapski, Bogusław Okopień
BACKGROUND: The immune system of humans is strongly affected by the processes of aging and what is called immunosenescence and inflammaging. Aging processes are also associated with altered macrophage functions and their ability to undergo differential activation. As a result, the risk of macrophage-related disorders like atherosclerosis is increased in the elderly. METHODS: Human monocyte-derived macrophages obtained from young and elderly healthy volunteers were stimulated with either lipopolysaccharide (LPS) or interleukin-4 (IL-4), and the expression of classical and alternative activation markers was assessed...
October 2014: Pharmacological Reports: PR
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