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Effect of ROS on peripheral blood cells

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https://www.readbyqxmd.com/read/28426671/effect-of-mitochondrially-targeted-carboxy-proxyl-nitroxide-on-akt-mediated-survival-in-daudi-cells-significance-of-a-dual-mode-of-action
#1
Gokul Variar, Tarun Pant, Apoorva Singh, Abinaya Ravichandran, Sushant Swami, Balaraman Kalyanaraman, Anuradha Dhanasekaran
Vicious cycles of mutations and reactive oxygen species (ROS) generation contribute to cancer progression. The use of antioxidants to inhibit ROS generation promotes cytostasis by affecting the mutation cycle and ROS-dependent survival signaling. However, cancer cells select mutations to elevate ROS albeit maintaining mitochondrial hyperpolarization (Δψm), even under hypoxia. From this perspective, the use of drugs that disrupt both ROS generation and Δψm is a viable anticancer strategy. Hence, we studied the effects of mitochondrially targeted carboxy proxyl nitroxide (Mito-CP) and a control ten carbon TPP moiety (Dec-TPP+) in the human Burkitt lymphoma cell line (Daudi) and normal peripheral blood mononuclear cells under hypoxia and normoxia...
2017: PloS One
https://www.readbyqxmd.com/read/28421266/reactive-oxygen-species-mediated-t-lymphocyte-abnormalities-in-an-iron-overloaded-mouse-model-and-iron-overloaded-patients-with-myelodysplastic-syndromes
#2
Jie Chen, Wen-Yi Lu, Ming-Feng Zhao, Xiao-Li Cao, Yan-Yu Jiang, Xin Jin, Ping Xu, Ting-Ting Yuan, Yu-Chen Zhang, Xiao Chai, Juan-Xia Meng, Qing Li, Xia Xiao, Juan Mu, De-Guan Li, Ai-Ping Qi
The adverse effects of iron overload have raised more concerns as a growing number of studies reported its association with immune disorders. This study aimed to investigate alterations in the immune system by iron overload in patients with myelodysplastic syndrome (MDS) and an iron-overloaded mouse model. The peripheral blood from patients was harvested to test the effect of iron overload on the subsets of T lymphocytes, and the level of reactive oxygen species (ROS) was also evaluated. The data showed that iron-overloaded patients had a lower percentage of CD3(+) T cells and disrupted T cell subsets, concomitant with higher ROS level in lymphocytes...
April 18, 2017: Annals of Hematology
https://www.readbyqxmd.com/read/28331925/biological-effects-of-ticagrelor-over-clopidogrel-in-patients-with-stable-coronary-artery-disease-and-chronic-obstructive-pulmonary-disease
#3
Gianluca Campo, Francesco Vieceli Dalla Sega, Rita Pavasini, Giorgio Aquila, Francesco Gallo, Francesca Fortini, Elisabetta Tonet, Paolo Cimaglia, Annamaria Del Franco, Gabriele Pestelli, Alessandro Pecoraro, Marco Contoli, Cristina Balla, Simone Biscaglia, Paola Rizzo, Roberto Ferrari
Patients with SCAD and concomitant COPD are at high risk of cardiovascular adverse events, due to chronic inflammation, responsible of endothelial dysfunction, oxidative stress and heightened platelet reactivity (PR). The objective of this randomised clinical trial was to test if ticagrelor is superior to clopidogrel in improving endothelial function in patients with stable coronary artery disease (SCAD) and concomitant chronic obstructive pulmonary disease (COPD). Forty-six patients with SCAD and COPD undergoing percutaneous coronary intervention (PCI) were randomly assigned to receive clopidogrel (n=23) or ticagrelor (n=23) on top of standard therapy with aspirin...
March 23, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28260045/a-herbal-formula-sykt-reverses-doxorubicin%C3%A2-induced-myelosuppression-and-cardiotoxicity-by-inhibiting-ros%C3%A2-mediated-apoptosis
#4
Ting Chen, Hong-Mei Shen, Zhi-Yong Deng, Zuo-Zhang Yang, Rui-Lian Zhao, Li Wang, Zhi-Ping Feng, Chao Liu, Wen-Hui Li, Zhi-Jie Liu
Doxorubicin (DOX) is an antineoplastic drug widely used for the treatment of various types of cancer; however, it can induce severe side effects, such as myelosuppression and cardiotoxicity. Sanyang Xuedai (SYKT) is a natural medicine originating from an ancient prescription of the Dai nationality in Southwest China. With eight Chinese herbal medicines, including sanguis draconis, radix et rhizoma notoginseng, radix et rhizoma glycyrrhizae and radix angelicae sinensis as the primary ingredients, SYKT has been reported to possess numerous biological functions...
April 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28245422/-underlying-mechanisms-of-iron-overloading-in-myelodysplastic-syndrome-review
#5
Qing-Qing Zheng, Chun-Kang Chang
Myelodysplastic Syndromes(MDS) comprise a heterogenous group of hematopoietic stem cell malignancies characterized by peripheral cytopenias and have a substantial risk of progression to acute myeloid leukemia(AML). MDS, without effective cure methods, is one of the common hematologic malignant tumors with great threaten to people's health. The phenomenon of iron overloading is common in MDS, which has a poor effect on overall survival and leukemic progression to MDS but get good prognosis by iron chelation therapy...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28242497/toxicological-effects-of-graphene-oxide-on-adult-zebrafish-danio-rerio
#6
Jaqueline P Souza, Jéssica F Baretta, Fabrício Santos, Ieda M M Paino, Valtencir Zucolotto
Graphene exhibits unique physical and chemical properties that facilitate its application in many fields, including electronics and biomedical areas. However, the use of graphene and its derivatives could result in accumulation in aquatic environments, and the risks posed by these compounds for organisms are not completely understood. In this study, we investigated the effects of graphene oxide (GO) on adult zebrafish (Danio rerio). Experimental fish were exposed to 2, 10 or 20mgL(-1) GO, and the cytotoxicity, genotoxicity and oxidative stress were assessed...
February 16, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28216895/vincamine-alleviates-amyloid-%C3%AE-25-35-peptides-induced-cytotoxicity-in-pc12-cells
#7
Jianfeng Han, Qiumin Qu, Jin Qiao, Jie Zhang
OBJECTIVE: Vincamine is a plant alkaloid used clinically as a peripheral vasodilator that increases cerebral blood flow and oxygen and glucose utilization by neural tissue to combat the effect of aging. The main purpose of the present study is to investigate the influence of vincamine on amyloid-β 25-35 (Aβ25-35) induced cytotoxicity, to gain a better understanding of the neuroprotective effects of this clinically used anti-Alzheimer's disease drug. MATERIALS AND METHODS: Oxidative stress was assessed by measuring malondialdehyde, glutathione, and superoxide dismutase (SOD) levels...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28181167/adherence-to-mediterranean-diet-is-associated-with-methylation-changes-in-inflammation-related-genes-in-peripheral-blood-cells
#8
A Arpón, J I Riezu-Boj, F I Milagro, C Razquin, M A Martínez-González, D Corella, R Estruch, R Casas, M Fitó, E Ros, J Salas-Salvadó, J A Martínez
Epigenetic processes, including DNA methylation, might be modulated by environmental factors such as the diet, which in turn have been associated with the onset of several diseases such as obesity or cardiovascular events. Meanwhile, Mediterranean diet (MedDiet) has demonstrated favourable effects on cardiovascular risk, blood pressure, inflammation and other complications related to excessive adiposity. Some of these effects could be mediated by epigenetic modifications. Therefore, the objective of this study was to investigate whether the adherence to MedDiet is associated with changes in the methylation status from peripheral blood cells...
February 8, 2017: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28115023/astaxanthin-attenuates-total-body-irradiation-induced-hematopoietic-system-injury-in-mice-via-inhibition-of-oxidative-stress-and-apoptosis
#9
Xiao-Lei Xue, Xiao-Dan Han, Yuan Li, Xiao-Fei Chu, Wei-Min Miao, Jun-Ling Zhang, Sai-Jun Fan
BACKGROUND: The hematopoietic system is especially sensitive to total body irradiation (TBI), and myelosuppression is one of the major effects of TBI. Astaxanthin (ATX) is a powerful natural anti-oxidant with low toxicity. In this study, the effect of ATX on hematopoietic system injury after TBI was investigated. METHODS: Flow cytometry was used to detect the proportion of hematopoietic progenitor cells (HPCs) and hematopoietic stem cells (HSCs), the level of intracellular reactive oxygen species (ROS), expression of cytochrome C, cell apoptosis, and NRF2-related proteins...
January 23, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28105091/effect-of-melatonin-on-the-peripheral-t-lymphocyte-cell-cycle-and-levels-of-reactive-oxygen-species-in-patients-with-premature-ovarian-failure
#10
Yanmin Li, Hongli Liu, Jing Sun, Yipeng Tian, Changzhong Li
The objective of the present study was to observe the curative effect and mechanism of melatonin for suppression of premature ovarian failure (POF). From December 2014 to June 2015, 128 patients were consecutively diagnosed with POF in the Department of Gynaecology and Obstetrics. The patients were randomly divided into the experimental and control groups. The experimental group received melatonin tablets (1-3 mg/day), while the control group received placebo tablets. The levels of six sex hormones, percentage of T lymphocytes in the G1/M phase, and levels of reactive oxygen species (ROS) were determined at four different time-points (1 day before treatment, and at 1, 3 and 6 months after treatment) in both groups...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28089725/high-concentration-of-branched-chain-amino-acids-promotes-oxidative-stress-inflammation-and-migration-of-human-peripheral-blood-mononuclear-cells-via-mtorc1-activation
#11
Olha Zhenyukh, Esther Civantos, Marta Ruiz-Ortega, Maria Soledad Sánchez, Clotilde Vázquez, Concepción Peiró, Jesús Egido, Sebastián Mas
Leucine, isoleucine and valine are essential aminoacids termed branched-chain amino acids (BCAA) due to its aliphatic side-chain. In several pathological and physiological conditions increased BCAA plasma concentrations have been described. Elevated BCAA levels predict insulin resistance development. Moreover, BCAA levels higher than 2mmol/L are neurotoxic by inducing microglial activation in maple syrup urine disease. However, there are no studies about the direct effects of BCAA in circulating cells. We have explored whether BCAA could promote oxidative stress and pro-inflammatory status in peripheral blood mononuclear cells (PBMCs) obtained from healthy donors...
March 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28081460/blood-brain-barrier-dysfunction-induced-by-silica-nps-in%C3%A2-vitro-and-in%C3%A2-vivo-involvement-of-oxidative-stress-and-rho-kinase-jnk-signaling-pathways
#12
Xin Liu, Baiyan Sui, Jiao Sun
Silica nanoparticles (SiO2-NPs) has been extensively exploited in biomedical fields and mostly designed to enter the circulatory system, however, few studies focused on the potential adverse effects of SiO2-NPs exposure on the blood-brain barrier (BBB) that serves as a critical barrier between the central nervous system (CNS) and the peripheral circulation. This study attempts to provide an understanding of whether and how SiO2-NPs disrupts the BBB in vitro and in vivo. Through a human BBB model, we found that SiO2-NPs could induce tight junction loss and cytoskeleton arrangement, and increase inflammatory response and the release of vascular endothelial growth factor (VEGF) of brain microvessel endothelial cells (BMECs), which further activates astrocytes to amplify the generation of VEGF and increase the aquaporin-4 expression, and thus causing BBB disruption through a complex immunoregulatory loop between BMECs and astrocytes under SiO2-NPs exposure...
March 2017: Biomaterials
https://www.readbyqxmd.com/read/28078186/soluble-receptor-for-advanced-glycation-end-products-improves-stromal-cell-derived-factor-1-activity-in-model-diabetic-environments
#13
Melissa Przyborowski Olekson, Renea A Faulknor, Henry C Hsia, Ann Marie Schmidt, François Berthiaume
Objective: In diabetes, hyperglycemia causes the accumulation of advanced glycation end products (AGEs) that trigger reactive oxygen species (ROS) generation through binding the receptor for AGEs (RAGE). Because exogenous growth factors have had little success in enhancing chronic wound healing, we investigated whether hyperglycemia-induced AGEs interfere with cellular responses to extracellular signals. We used stromal cell-derived factor-1 (SDF-1), an angiogenic chemokine also known to promote stem cell recruitment in skin wounds...
December 1, 2016: Advances in Wound Care
https://www.readbyqxmd.com/read/28065629/the-effects-of-emodin-on-cell-viability-respiratory-burst-and-gene-expression-of-nrf2-keap1-signaling-molecules-in-the-peripheral-blood-leukocytes-of-blunt-snout-bream-megalobrama-amblycephala
#14
Zhenxin Zhao, Jun Xie, Bo Liu, Xianping Ge, Changyou Song, Mingchun Ren, Qunlan Zhou, Linghong Miao, Huimin Zhang, Fan Shan, Zhenfei Yang
We determined the effects of emodin on the cell viability, respiratory burst activity, mRNA levels of antioxidative enzymes (Cu-Zn SOD, CAT and NOX2), and gene expressions of the Nrf2-Keap1 signaling molecules in the peripheral blood leukocytes of blunt snout bream. Triplicate groups of cultured cells were treated with different concentrations of emodin (0.04-25 μg/ml) for 24 h. Results showed that the emodin caused a dramatic loss in cell viability, and occurred in a dose-dependent manner. Emodin exposure (1-25 μg/ml) were significantly induced the ROS generation compared to the control...
March 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28058665/oxidative-stress-and-genotoxicity-of-zinc-oxide-nanoparticles-to-pseudomonas-species-human-promyelocytic-leukemic-hl-60-and-blood-cells
#15
Deepika Soni, Deepa Gandhi, Prashant Tarale, Amit Bafana, R A Pandey, Saravanadevi Sivanesan
In the present study, toxicity of commercial zinc oxide nanoparticles (ZnO NPs) was studied on the bacterium Pseudomonas sp., human promyelocytic leukemia (HL-60) cells, and peripheral blood mononuclear cells (PBMC). The toxicity was assessed by measuring growth, cell viability, and protein expression in bacterial cell. The bacterial growth and viability decreased with increasing concentrations of ZnO NP. Three major proteins, ribosomal protein L1 and L9 along with alkyl hydroperoxides reductase, were upregulated by 1...
January 5, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/27988860/the-mitochondrial-apoptotic-pathway-is-induced-by-cu-ii-antineoplastic-compounds-casiope%C3%A3-nas-%C3%A2-in-sk-n-sh-neuroblastoma-cells-after-short-exposure-times
#16
Juan Carlos García-Ramos, Anllely Grizett Gutiérrez, Adriana Vázquez-Aguirre, Yanis Toledano-Magaña, Ana Luisa Alonso-Sáenz, Virginia Gómez-Vidales, Marcos Flores-Alamo, Carmen Mejía, Lena Ruiz-Azuara
The family of Copper(II) coordination compounds Casiopeínas(®) (Cas) has shown antiproliferative activity in several tumour lines by oxidative cellular damage and mitochondrial dysfunction that lead to cell death through apoptotic pathways. The goal of this work is looking for the functional mechanism of CasIIgly, CasIIIia and CasIIIEa in neuroblastoma metastatic cell line SK-N-SH, a paediatric extra-cranial tumour which is refractory to several anti-carcinogenic agents. All Cas have shown higher antiproliferative activity than cisplatin (IC50 = 123 μM) with IC50 values of 18, 22 and 63 µM for CasIIgly, CasIIIEa and CasIIIia, respectively...
February 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/27932441/trophoblast-cells-inhibit-neutrophil-extracellular-trap-formation-and-enhance-apoptosis-through-vasoactive-intestinal-peptide-mediated-pathways
#17
Guillermina Calo, Florencia Sabbione, Daiana Vota, Daniel Paparini, Rosanna Ramhorst, Analía Trevani, Claudia Pérez Leirós
STUDY QUESTION: Do human trophoblast cells modulate neutrophil extracellular trap (NET) formation, reactive oxygen species (ROS) synthesis and neutrophil apoptosis through mechanisms involving vasoactive intestinal peptide (VIP)? SUMMARY ANSWER: Trophoblast cells inhibited NET formation and ROS synthesis and enhanced neutrophil apoptosis through VIP-mediated pathways in a model of maternal-placental interaction. WHAT IS KNOWN ALREADY: Immune homeostasis maintenance at the maternal-placental interface is mostly coordinated by trophoblast cells...
January 2017: Human Reproduction
https://www.readbyqxmd.com/read/27815458/polymorphism-in-murine-mtatp8-gene-correlates-with-decreased-reactive-oxygen-species-in-aging-hematopoietic-cells
#18
Catrin Roolf, Christin Kretzschmar, Katrin Timmer, Anett Sekora, Gudrun Knübel, Hugo Murua Escobar, Georg Fuellen, Saleh M Ibrahim, Markus Tiedge, Simone Baltrusch, Sarah Müller, Rüdiger Köhling, Christian Junghanss
BACKGROUND: Mitochondrial DNA (mtDNA) encodes for the respiratory chain proteins. Genetic alterations in mtDNA have been described during aging and linked to impaired hematopoiesis. MATERIALS AND METHODS: We investigated two novel conplastic mouse strains harboring a mitochondrial nt7778 G/T polymorphism leading to an amino acid exchange in respiratory chain complex V. Effects on reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, as well as bone marrow composition and peripheral blood counts, were investigated during aging (up to 24 month)...
November 2016: In Vivo
https://www.readbyqxmd.com/read/27809262/benzene-exposure-alters-expression-of-enzymes-involved-in-fatty-acid-%C3%AE-oxidation-in-male-c3h-he-mice
#19
Rongli Sun, Meng Cao, Juan Zhang, Wenwen Yang, Haiyan Wei, Xing Meng, Lihong Yin, Yuepu Pu
Benzene is a well-known hematotoxic carcinogen that can cause leukemia and a variety of blood disorders. Our previous study indicated that benzene disturbs levels of metabolites in the fatty acid β-oxidation (FAO) pathway, which is crucial for the maintenance and function of hematopoietic and leukemic cells. The present research aims to investigate the effects of benzene on changes in the expression of key enzymes in the FAO pathway in male C3H/He mice. Results showed that benzene exposure caused reduced peripheral white blood cell (WBC), red blood cell (RBC), platelet (Pit) counts, and hemoglobin (Hgb) concentration...
October 31, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27796742/brain-oxidative-stress-during-experimental-sepsis-is-attenuated-by-simvastatin-administration
#20
Carlos Henrique Rocha Catalão, Nilton Nascimento Santos-Júnior, Luís Henrique Angenendt da Costa, Anderson Oliveira Souza, Luciane Carla Alberici, Maria José Alves Rocha
During sepsis, brain damage is associated with oxidative stress due to overproduction of reactive oxygen species (ROS). Although there are recent reports about the benefits of statins in experimental sepsis and endotoxemia in peripheral organs, little is known about their effects in the CNS. Here, we investigated the antioxidant properties of simvastatin and its possible neuroprotective role during experimental sepsis. Male Wistar rats (250-300 g) were submitted to cecal ligation and puncture (CLP, n = 34) or remained as non-manipulated (naive, n = 34)...
October 28, 2016: Molecular Neurobiology
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