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https://www.readbyqxmd.com/read/28917785/fluorescence-quenching-based-alkaline-phosphatase-activity-detection
#1
Yaqi Mei, Qiong Hu, Baojing Zhou, Yonghui Zhang, Minhui He, Ting Xu, Feng Li, Jinming Kong
Simple and fast detection of alkaline phosphatase (ALP) activity is of great importance for diagnostic and analytical applications. In this work, we report a turn-off approach for the real-time detection of ALP activity on the basis of the charge transfer induced fluorescence quenching of the Cu(BCDS)2(2-) (BCDS = bathocuproine disulfonate) probe. Initially, ALP can enzymatically hydrolyze the substrate ascorbic acid 2-phosphate to release ascorbic acid (AA). Subsequently, the AA-mediated reduction of the Cu(BCDS)2(2-) probe, which displays an intense photoluminescence band at the wavelength of 402nm, leads to the static quenching of fluorescence of the probe as a result of charge transfer...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28910417/differential-cellular-responses-associated-with-oxidative-stress-and-cell-fate-decision-under-nitrate-and-phosphate-limitations-in-thalassiosira-pseudonana-comparative-proteomics
#2
Qun Lin, Jun-Rong Liang, Qian-Qian Huang, Chun-Shan Luo, Donald M Anderson, Chris Bowler, Chang-Ping Chen, Xue-Song Li, Ya-Hui Gao
Diatoms are important components of marine ecosystems and contribute greatly to the world's primary production. Despite their important roles in ecosystems, the molecular basis of how diatoms cope with oxidative stress caused by nutrient fluctuations remains largely unknown. Here, an isobaric tags for relative and absolute quantitation (iTRAQ) proteomic method was coupled with a series of physiological and biochemical techniques to explore oxidative stress- and cell fate decision-related cellular and metabolic responses of the diatom Thalassiosira pseudonana to nitrate (N) and inorganic phosphate (P) stresses...
2017: PloS One
https://www.readbyqxmd.com/read/28904636/the-orthopaedic-management-of-lower-limb-deformity-in-hypophosphataemic-rickets
#3
A Horn, J Wright, D Bockenhauer, W Van't Hoff, D M Eastwood
BACKGROUND: Many patients with X-linked hypophosphataemic rickets (X-LHPR) demonstrate significant lower limb deformity despite optimal medical management. This study evaluates the use of guided growth by means of hemi-epiphysiodesis to address coronal plane deformity in the skeletally immature child. METHODS: Since 2005, 24 patients with X-LHPR have been referred to our orthopaedic unit for evaluation. All patients had standardised long leg radiographs that were analysed sequentially before and after surgery if any was performed...
August 1, 2017: Journal of Children's Orthopaedics
https://www.readbyqxmd.com/read/28904570/bone-mineral-status-in-children-with-epilepsy-biochemical-and-radiologic-markers
#4
Bothina Hasaneen, Riad Moustafa Elsayed, Nanees Salem, Ashraf Elsharkawy, Noha Tharwat, Khaled Fathy, Amany El-Hawary, Hadil M Aboelenin
OBJECTIVE: The aim of this study is to assess bone mineral status in children with epilepsy, on different antiepileptic drugs (AEDs) regimen, using dual-energy X-ray absorptiometry (DXA) and routine biochemical bone markers. PATIENTS AND METHODS: This is observational prospective controlled cohort study, conducted at Mansoura University Children Hospital, from January 2014 to June 2015. In this study, we had 152 participants with ages 3-13 years, 70 children diagnosed with epilepsy and 82 were controls...
April 2017: Journal of Pediatric Neurosciences
https://www.readbyqxmd.com/read/28902664/comparison-of-the-inhibitory-mechanisms-of-diethyl-citrate-sodium-citrate-and-phosphonoformic-acid-on-calcification-induced-by-high-inorganic-phosphate-contents-in-mouse-aortic-smooth-muscle-cells
#5
Jian-Ming Ouyang, Chao-Yang Duan, Chong-Yu Zhang, Xin-Yuan Sun, Bao-Song Gui, Da Guo
OBJECTIVE: This study aimed to investigate the differences and inhibitory effects of diethyl citrate (Et2Cit), sodium citrate (Na3Cit), and phosphonoformic acid (PFA) on calcification induced by high inorganic phosphate (Pi) contents in mouse aortic smooth muscle cells (MOVAS) and to develop drugs that can induce anticoagulation and inhibit vascular calcification (VC). METHODS: Alive and fixed MOVAS were assessed for 14 days in the presence of high Pi with increasing Et2Cit, Na3Cit, and PFA concentrations...
September 11, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28900819/a-homozygous-pigo-mutation-associated-with-severe-infantile-epileptic-encephalopathy-and-corpus-callosum-hypoplasia-but-normal-alkaline-phosphatase-levels
#6
Yoav Zehavi, Anja von Renesse, Etty Daniel-Spiegel, Yonatan Sapir, Luci Zalman, Ilana Chervinsky, Markus Schuelke, Rachel Straussberg, Ronen Spiegel
We describe two sisters from a consanguineous Arab family with global developmental delay, dystrophy, axial hypotonia, epileptic encephalopathy dominated by intractable complex partial seizures that were resistant to various anti-epileptic treatments. Dysmorphic features comprised low set ears, hypertelorism, upslanting palpebral fissures, a broad nasal bridge, and blue sclera with elongated eyelashes. Brain MRI in both children showed a corpus callosum hypoplasia that was evident already in utero and evolving cortical atrophy...
September 13, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28892757/11-nor-9-carboxy-%C3%AE-9-tetrahydrocannabinol-glucuronide-exhibits-acyl-migration-isomers
#7
Stephanie Hanisch, Alexander Paulke, Stefan W Toennes
11-nor-Δ(9)-Tetrahydrocannabinol-9-carboxylic acid glucuronide (THCCOOH-glucuronide) is an 1-β-O-acyl glucuronide which degrades not only to 11-nor-9-carboxy-Δ(9)-THC (THCCOOH) but, additionally, to an isomer with a currently unknown structure. The present study was carried out to examine whether acyl glucuronide isomers are formed by acyl migration and if they are involved in formation of this isomer. THCCOOH-glucuronide was incubated in phosphate buffer (pH 7.4, 37°C, 7days) and a variety of glucuronide cleavage procedures were performed...
August 31, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28891014/phosphate-arsenate-relations-to-affect-arsenic-concentration-in-plant-tissues-growth-and-antioxidant-efficiency-of-sunflower-helianthus-annuus-l-under-arsenic-stress
#8
Waqas Azeem, Muhammad Ashraf, Sher Muhammad Shahzad, Muhammad Imtiaz, Mumtaz Akhtar, Muhammad Shahid Rizwan
Relations between phosphate and arsenate are important but inconsistent to influence arsenic (As) phytotoxicity depending on many plant and soil factors. Present research aimed to investigate the phosphate and arsenate interactions in sunflower (Helianthus annuus L.) grown in alkaline calcareous soil for 18 weeks under natural environmental conditions at three arsenate [0 (As0), 40 (As40), and 80 (As80) mg As kg(-1) soil as sodium arsenate] and three phosphate [0 (P0), 100 (P100), and 200 (P200) mg P2O5 kg(-1) soil as diammonium phosphate] levels...
September 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28888859/determination-of-heavy-metal-toxicity-by-using-a-micro-droplet-hydrodynamic-voltammetry-for-microalgal-bioassay-based-on-alkaline-phosphatase
#9
Md Saiful Islam, Kazuto Sazawa, Noriko Hata, Kazuharu Sugawara, Hideki Kuramitz
We developed an electrochemical microalgal bioassay for the determination of heavy metal toxicity in water on the basis of the alkaline phosphatase (ALP) enzyme inhibition of Chlamydomonas reinhardtii. Five heavy metals were chosen as toxicants: Hg, Cd, Pb, Zn, and Cu. The induced ALP activity of C. reinhardtii was inhibited using the phosphate starvation method, and the results were evaluated by measuring the electrochemical oxidation of p-aminophenol (PAP) following the enzymatic conversion of p-aminophenyl phosphate (PAPP) as a substrate...
September 4, 2017: Chemosphere
https://www.readbyqxmd.com/read/28888853/monitoring-guidance-for-patients-with-hypophosphatasia-treated-with-asfotase-alfa
#10
REVIEW
Priya S Kishnani, Eric T Rush, Paul Arundel, Nick Bishop, Kathryn Dahir, William Fraser, Paul Harmatz, Agnès Linglart, Craig F Munns, Mark E Nunes, Howard M Saal, Lothar Seefried, Keiichi Ozono
Hypophosphatasia (HPP) is a rare, inherited, systemic, metabolic disorder caused by autosomal recessive mutations or a single dominant-negative mutation in the gene encoding tissue-nonspecific alkaline phosphatase (TNSALP). The disease is associated with a broad range of signs, symptoms, and complications, including impaired skeletal mineralization, altered calcium and phosphate metabolism, recurrent fractures, pain, respiratory problems, impaired growth and mobility, premature tooth loss, developmental delay, and seizures...
July 25, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28888067/injectable-thermosensitive-alginate-%C3%AE-tricalcium-phosphate-aspirin-hydrogels-for-bone-augmentation
#11
Xiaoqian Fang, Lei Lei, Ting Jiang, Ying Chen, Yunqing Kang
In this study, an injectable and thermo-sensitive alginate/β-tricalcium phosphate hydrogel (TSAH/β-TCP) was prepared for aspirin release to a bone defect. Aspirin was dissolved into a mixture of poly(N-isopropylacrylamide) (PNIPAAm), an aminated alginate-g-PNIPAAm co-polymer, and β-TCP powders. Scanning electron microscopy showed that TSAH/β-TCP had an interconnected porous microstructure with a porosity of 86.78%. The cross-linked hydrogel released approximately 40% of the aspirin in the first 3 days and then slowly released the remainder...
September 9, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28887983/effect-of-pores-formation-process-and-oxygen-plasma-treatment-to-hydroxyapatite-formation-on-bioactive-peek-prepared-by-incorporation-of-precursor-of-apatite
#12
Takeshi Yabutsuka, Keito Fukushima, Tomoko Hiruta, Shigeomi Takai, Takeshi Yao
When bioinert substrates with fine-sized pores are immersed in a simulated body fluid (SBF) and the pH value or the temperature is increased, fine particles of calcium phosphate, which the authors denoted as 'precursor of apatite' (PrA), are formed in the pores. By this method, hydroxyapatite formation ability can be provided to various kinds of bioinert materials. In this study, the authors studied fabrication methods of bioactive PEEK by using the above-mentioned process. First, the fine-sized pores were formed on the surface of the PEEK substrate by H2SO4 treatment...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28887974/mechanical-properties-and-osteogenic-activity-of-poly-l-lactide-fibrous-membrane-synergistically-enhanced-by-chitosan-nanofibers-and-polydopamine-layer
#13
Hua Liu, Wenling Li, Wei Wen, Binghong Luo, Mingxian Liu, Shan Ding, Changren Zhou
To synergistically improve the mechanical properties and osteogenic activity of electrospinning poly(l-lactide) (PLLA) membrane, chitosan (CS) nanofibers were firstly introduced to prepare sub-micro and nanofibers interpenetrated PLLA/CS membrane, which was further surface modified with a polydopamine (PDA) layer to obtain PLLA/CS-PDA. Surface morphology, porosity, surface area and hydrophilicity of the obtained fibrous membranes were studied in detail. As compared to pure PLLA, the significant increase in the mechanical properties of the PLLA/CS, and especially of the PLLA/CS-PDA, was confirmed by tensile testing both in dry and wet states...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28880715/defective-mineralization-in-x-linked-hypophosphatemia-dental-pulp-cell-cultures
#14
B R Coyac, B Hoac, P Chafey, G Falgayrac, L Slimani, P S Rowe, G Penel, A Linglart, M D McKee, C Chaussain, C Bardet
X-linked hypophosphatemia (XLH) is a skeletal disease caused by inactivating mutations in the PHEX gene. Mutated or absent PHEX protein/enzyme leads to a decreased serum phosphate level, which cause mineralization defects in the skeleton and teeth (osteomalacia/odontomalacia). It is not yet altogether clear whether these manifestations are caused solely by insufficient circulating phosphate availability for mineralization or also by a direct, local intrinsic effect caused by impaired PHEX activity. Here, we evaluated the local role of PHEX in a 3-dimensional model of extracellular matrix (ECM) mineralization...
September 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28879686/development-of-a-bioactive-porous-collagen-%C3%AE-tricalcium-phosphate-bone-graft-assisting-rapid-vascularization-for-bone-tissue-engineering-applications
#15
Nafiseh Baheiraei, Mohamma Reza Nourani, Sm Javad Mortazavi, Mansoureh Movahedin, Hossein Eyni, Fatemeh Baghery, Mohammad Hadi Norahan
We developed collagen (COL) and collagen/beta tricalcium phosphate (COL/β-TCP) scaffolds with a β-TCP/collagen weight ratio of 4 by freeze-drying. Mouse bone marrow-derived mesenchymal stem cells (BMMSCs) were cultured on these scaffolds for 14 days. Samples were characterized by physicochemical analyses and their biological properties such as cell viability and alkaline phosphatase (ALP) activity was,also, examined. Additionally, the vascularization potential of the prepared scaffolds was tested subcutaneously in Wistar rats...
September 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28877641/collagen-and-hydroxyapatite-scaffolds-activate-distinct-osteogenesis-signaling-pathways-in-adult-adipose-derived-multipotent-stromal-cells
#16
Wei Duan, Masudul Haque, Michael T Kearney, Mandi Lopez
Osteogenic cell signaling pathway disruption varies among bone diseases. This investigation was designed to identify adipose derived multipotent stromal cell (ASC) and bone graft scaffold combinations for local, targeted restoration of gene expression and extracellular matrix (ECM) deposition. Human ASC osteogenesis on bone graft materials was quantified following culture in stromal (S), osteogenic (O) or osteogenic for 48 hours followed by stromal medium (OS) to test the two-part hypothesis: 1) Identical ASC isolates on distinct bone graft scaffolds demonstrate unique viability, differentiation, ECM production and gene expression in the same culture conditions; 2) Identical ASC-bone graft scaffold combinations have different cell viability, differentiation, ECM production, and gene expression when cultured in S, O or OS medium...
September 6, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28872843/diffusion-controllable-biomineralization-conducted-in-situ-in-hydrogels-based-on-reversibly-cross-linked-hyperbranched-polyglycidol
#17
Mateusz Gosecki, Slawomir Kazmierski, Monika Gosecka
We present biocompatible hydrogel systems suitable for biomineralization processes based on hyperbranched polyglycidol cross-linked with acrylamide copolymer bearing carbonyl-coordinated boronic acid. At neutral pH, diol functional groups of HbPGL react with boronic acid of polyacrylamide to generate 3D network in water by the formation of boronic ester cross-links. The dynamic associative/dissociative characteristics of the cross-links makes the network reversible. The presented hydrogels display self-healing properties and are injectable, facilitating gap filing of bone tissue...
September 13, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28869710/influence-of-hydrodynamic-pressure-on-the-proliferation-and-osteogenic-differentiation-of-bone-mesenchymal-stromal-cells-seeded-on-polyurethane-scaffolds
#18
Xiangyu Tang, Songsong Teng, Chaoxu Liu, Michael Jagodzinski
Hydraulic pressure has recently been introduced as an effective stimulation in the field of tissue engineering. In the present study, a polymer scaffold consisting of polyurethane (PU)-based 1, 4-butanediisocyanate (BDI) was fabricated. A self-designed bioreactor was employed to produce perfusion and hydrodynamic pressure stimulations. The viability, proliferation and osteogenic differentiation of the rat bone mesenchymal stromal cell (rBMSC) growing in the polymer scaffold were investigated after hydrodynamic pressure stimulation...
September 4, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28869707/spio-au-core-shell-nanoparticles-for-promoting-osteogenic-differentiation-of-mc3t3-e1-cells-concentration-dependence-study
#19
Muzhaozi Yuan, Ya Wang, Yi-Xian Qin
This work aims to explore the concentration-dependence of SPIO-Au core-shell NPs (17.3 ± 1.2 nm in diameter) on biocompatibility and osteogenic differentiation of preosteoblast MC3T3-E1 cells. The stability of NPs was first investigated by UV-Vis absorption spectra and zeta potential measurement. Then concentration effects of NPs (1-80 μg/mL) were evaluated on viability, morphology, proliferation, cellular uptake and alkaline phosphate (ALP) activity levels. Results have shown strong stability and no acute toxicity (viability > 93%) or morphological difference at all concentration levels of NPs...
September 4, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28866400/valorisation-of-n-and-p-from-waste-water-by-using-natural-reactive-hybrid-sorbents-nutrients-n-p-k-release-evaluation-in-amended-soils-by-dynamic-experiments
#20
Diana Guaya, César Valderrama, Adriana Farran, Teresa Sauras, José Luis Cortina
The removal of nutrients (nitrogen (N), phosphorous (P)) from waste water has become a resource recovery option in recent regulations worldwide, as observed in the European Union. Although both of these nutrients could be recovered from the sludge line, >70-75% of the N and P is discharged into the water line. Efforts to improve the nutrient recovery ratios have focused on developing low-cost technologies that use sorption processes. In this study, a natural zeolite (clinoptilolite type) in its potassium (K) form was impregnated with hydrated metal oxides and used to prepare natural hybrid reactive sorbents (HRS) for the simultaneous recovery of ammonium (NH4(+)) and phosphate (PO4(3-)) from treated urban waste water...
August 31, 2017: Science of the Total Environment
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