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alkaline phosphate

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https://www.readbyqxmd.com/read/27936402/influence-of-triethyl-phosphate-on-phosphatase-activity-in-shooting-range-soil-isolation-of-a-zinc-resistant-bacterium-with-an-acid-phosphatase
#1
Sandra Story, Robin L Brigmon
Phosphatase-mediated hydrolysis of organic phosphate may be a viable means of stabilizing heavy metals via precipitation as a metal phosphate in bioremediation applications. We investigated the effect of triethyl phosphate (TEP) on soil microbial-phosphatase activity in a heavy-metal contaminated soil. Gaseous TEP has been used at subsurface sites for bioremediation of organic contaminants but not applied in heavy-metal contaminated areas. Little is known about how TEP affects microbial activity in soils and it is postulated that TEP can serve as a phosphate source in nutrient-poor groundwater and soil/sediments...
December 6, 2016: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27928636/effects-of-lanthanum-carbonate-and-calcium-carbonate-on-fibroblast-growth-factor-23-and-hepcidin-levels-in-chronic-hemodialysis-patients
#2
Yu-Ming Chang, Shih-Ching Tsai, Chih-Chung Shiao, Hung-Hsiang Liou, Chuan-Lan Yang, Nai-Yu Tung, Kua-Sui Hsu, I-Ling Chen, Mei-Chyn Liu, Jsun-Liang Kao, Rong-Na Jhen, Ya-Ting Huang
BACKGROUND: Phosphate binders have an impact on fibroblast growth factor 23 (FGF23); however, the effect of phosphate binders on serum hepcidin has not been explored. We conducted a 24-week multicenter randomized controlled trial to investigate the effects of lanthanum carbonate or calcium carbonate monotherapy on serum phosphate, FGF23, and hepcidin levels in chronic hemodialysis patients. METHODS: Forty-six patients were recruited, and daily dietary phosphorus was controlled between 600-800 mg...
December 7, 2016: Clinical and Experimental Nephrology
https://www.readbyqxmd.com/read/27928454/the-role-of-oral-sodium-bicarbonate-supplementation-in-maintaining-acid-base-balance-and-its-influence-on-the-cardiovascular-system-in-chronic-hemodialysis-patients-results-of-a-prospective-study
#3
Voiculeț C, Zară O, Bogeanu C, Văcăroiu I, Aron G
Background: Major acid-base variations during dialysis and the imbalances in serum calcium levels intensified by them play a role in cardiovascular damage of hemodialysis patients. Early vascular walls modifications can be objectified by determining the pulse wave velocity (PWV) - a marker of vascular stiffness that is associated with increased risk of cardiovascular events. Material and methods: This was a prospective study conducted on 63 chronic hemodialysis patients with diuresis above 500 mL/ 24 hours and predialysis blood pressure below 160 mmHg (treatment controlled) randomized in two groups for 12 months - the study group receiving interdialitic oral sodium bicarbonate doses and control group, without oral sodium bicarbonate supplementation, but receiving higher bicarbonate prescriptions in dialysis...
October 2016: Journal of Medicine and Life
https://www.readbyqxmd.com/read/27928449/severe-asymptomatic-coronary-obstruction-in-chronic-hemodialysed-patient-a-case-report
#4
Voiculeț C, Zara O, Văcăroiu I, Bogeanu C, Tiron T, Turcu F, Aron G, Ciocâlteu A
Introduction. Arterial stiffness and vascular calcifications are independent predictors of cardiovascular morbidity and mortality in the chronic kidney disease (CKD) stage 5D population. According to the guidelines, patients on renal replacement therapy represent a very high cardiovascular risk class. Case report. We report the case of a 67-year-old hypertensive male patient, known with CKD stage 5D on hemodialysis (three times per week), secondary bone mineral disease, admitted for progressive right leg pain...
October 2016: Journal of Medicine and Life
https://www.readbyqxmd.com/read/27916122/facile-colorimetric-assay-of-alkaline-phosphatase-activity-using-fe-ii-phenanthroline-reporter
#5
Qiong Hu, Baojing Zhou, Pengyun Dang, Lianzhi Li, Jinming Kong, Xueji Zhang
We report a versatile approach for the colorimetric assay of alkaline phosphatase (ALP) activity based on the distinctive metal-to-ligand charge-transfer (MLCT) absorption properties of Fe(II)-phenanthroline reporter. In the presence of ALP, the applied substrate ascorbic acid 2-phosphate is enzymatically hydrolyzed to produce ascorbic acid, which then reduces Fe(3+) to Fe(2+). The complexation of Fe(2+) with the bathophenanthroline disulfonate (BPS) ligand generates a blood-red Fe(BPS)3(4-) reporter, which is characterized by an intense MLCT absorption band at 535 nm in the visible range...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27912988/sequential-electrodialytic-recovery-of-phosphorus-from-low-temperature-gasification-ashes-of-chemically-precipitated-sewage-sludge
#6
Raimon Parés Viader, Pernille Erland Jensen, Lisbeth M Ottosen, Jesper Ahrenfeldt, Henrik Hauggaard-Nielsen
Phosphorus recycling from secondary materials like sewage sludge ashes offers an alternative to mining of phosphates from primary resources and a mean to counteract the current phosphorous rock depletion concern. A separation of P from the bulk ash is normally required, due to its low plant availability and the presence of heavy metals. Previously, more than 80% of P was recovered from incineration sewage sludge ashes using a two-compartment electrodialytic cell. In contrast, the recovery was below 30% for ashes from low-temperature gasification using the same setup...
November 29, 2016: Waste Management
https://www.readbyqxmd.com/read/27906446/study-on-adhesion-proliferation-and-differentiation-of-osteoblasts-promoted-by-new-absorbable-bioactive-glass-injection-in-vitro
#7
A Axrap, J Wang, Y Liu, M Wang, A Yusuf
OBJECTIVE: The objective of this study was to evaluate adhesion, proliferation and differentiation of osteoblasts grown on absorbable bioactive glass-calcium phosphate cement injection (BG-CPC) materials in vitro. MATERIALS AND METHODS: BG-CPC composite biomaterial samples were prepared in vitro, for culture with MC3T3-E1 rat osteoblasts. Cells were divided into CPC, BG and BG-CPC treated groups. After cultivation for 3d, cells were stained with rhodamine-phalloidin and 4',6-diamidino-2-phenylindole (DAPI) and observed by fluorescence microscopy for osteoblast morphology on the surface of biomaterials...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27905661/artificial-cartilage-bio-matrix-formed-of-hyaluronic-acid-and-mg2-polyphosphate
#8
X Wang, M Ackermann, E Tolba, M Neufurth, F Wurm, Q Feng, S Wang, H C Schröder, W E Müller
Here we show that inorganic polyphosphate (polyP), a polyanionic metabolic regulator consisting of multiple phosphate residues linked by energy-rich phosphoanhydride bonds, is present in the synovial fluid. In a biomimetic approach, to enhance cartilage synthesis and regeneration, we prepared amorphous polyP microparticles with Mg2+ as counterions. The particles were characterised by X-ray diffraction (XRD), energy-dispersive X-ray (EDX) and Fourier transformed infrared spectroscopic (FTIR) analyses. Similar particles were obtained after addition of Mg2+ ions to a solution containing hyaluronic acid, as a major component of the synovial fluid, and soluble Na-polyP...
November 29, 2016: European Cells & Materials
https://www.readbyqxmd.com/read/27900695/function-based-metagenomic-library-screening-and-heterologous-expression-strategy-for-genes-encoding-phosphatase-activity
#9
Genis A Castillo Villamizar, Heiko Nacke, Rolf Daniel
The release of phosphate from inorganic and organic phosphorus compounds can be mediated enzymatically. Phosphate-releasing enzymes, comprising acid and alkaline phosphatases, are recognized as useful biocatalysts in applications such as plant and animal nutrition, bioremediation and diagnostic analysis. Metagenomic approaches provide access to novel phosphatase-encoding genes. Here, we describe a function-based screening approach for rapid identification of genes conferring phosphatase activity from small-insert and large-insert metagenomic libraries derived from various environments...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27897998/a-new-two-photon-ratiometric-fluorescent-probe-for-detecting-alkaline-phosphatase-in-living-cells
#10
Xiaohong Zhou, Yuren Jiang, Xiongjie Zhao, Yao Zhu
Alkaline phosphatase (ALP) is an important diagnostic indicator of many human diseases. To quantitatively track ALP in biosystems, herein, for the first time, we report an efficient two-photon ratiometric fluorescent probe, termed probe 1 and based on classic naphthalene derivatives with a donor-π-acceptor (D-π-A) structure and deprotection of the phosphoric acid moiety by ALP. The presence of ALP causes the cleave of the phosphate group from naphthalene derivatives and the phosphate group changes the ability of the intramolecular charge transfer (ICT) and remarkably alters the probe's photophysical properties, thus an obvious ratiometric signal with an isoemissive point is observed...
November 25, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27896489/effects-of-iron-on-physical-and-mechanical-properties-and-osteoblast-cell-interaction-in-%C3%AE-tricalcium-phosphate
#11
Sahar Vahabzadeh, Susmita Bose
Iron (Fe) is a vital element and its deficiency causes abnormal bone metabolism. We investigated the effects of Fe and its concentration in β-tricalcium phosphate (β-TCP) on physicomechanical properties and in vitro proliferation and differentiation of osteoblasts. Our results showed that Fe addition at concentrations of 0.5 wt.% (0.5 Fe-TCP) and 1.0 wt.% (1.0 Fe-TCP) inhibits the β-TCP to α-TCP phase transformation at sintering temperature of 1250 °C. Addition of 0.25 wt.% Fe (0.25 Fe-TCP) increased the compressive strength of β-TCP from 167...
November 28, 2016: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/27895866/liver-findings-in-patients-with-hereditary-hemorrhagic-telangiectasia
#12
Wenyan Song, Dawei Zhao, Hongjun Li, Jinli Ding, Ning He, Yu Chen
BACKGROUND: Hereditary hemorrhagic telangiectasia (HHT), also known as Osler-Weber-Rendu syndrome, is a rare autosomal dominant genetic vascular dysplasia. OBJECTIVES: The aim is to characterize the liver involvement of HHT by multi-detector helical computed tomography. PATIENTS AND METHODS: Through the use of multi-detector helical computed tomography, scan data derived from 12 cases of HHT were retrospectively studied, and an abdominal scan was performed on all of the patients...
October 2016: Iranian Journal of Radiology: a Quarterly Journal Published By the Iranian Radiological Society
https://www.readbyqxmd.com/read/27886771/a-label-free-electrochemical-biosensor-for-microrna-detection-based-on-catalytic-hairpin-assembly-and-in-situ-formation-of-molybdophosphate
#13
Wei Cai, Shunbi Xie, Ying Tang, Yaqin Chai, Ruo Yuan, Jin Zhang
A label-free electrochemical biosensor for sensitive detection of miRNA-155 was presented by coupling enzyme-catalyzed in situ generation of electronic mediator for signal introduction with catalytic hairpin assembly (CHA) induced target recycling amplification strategy. In this work, alkaline phosphatase (ALP) was adopted to hydrolyze inactive substrate 1-naphthyl phosphate (NPP) to produce phosphate ion (PO4(3-)), which could further react with acidic molybdate to form abundant molybdophosphate anion (PMo12O40(3-)) on the surface of electrode...
January 15, 2017: Talanta
https://www.readbyqxmd.com/read/27886764/sensitive-and-selective-colorimetric-assay-of-alkaline-phosphatase-activity-with-cu-ii-phenanthroline-complex
#14
Qiong Hu, Minhui He, Yaqi Mei, Wenjie Feng, Su Jing, Jinming Kong, Xueji Zhang
Alkaline phosphatase (ALP) plays a vital role in dephosphorylation- and phosphorylation-related cellular regulation and signaling processes. Accordingly, the development of efficient methods for ALP activity assay is of significant importance in clinical diagnosis. In this work, a simple and practical method is reported for the first time for the sensitive and selective colorimetric assay of ALP activity by exploiting a water-soluble Cu(II)-phenanthroline complex as the probe, on the basis of the distinctive metal-to-ligand charge-transfer (MLCT) properties...
January 15, 2017: Talanta
https://www.readbyqxmd.com/read/27883077/tgf-beta-receptor-ii-interacting-protein-1-an-intracellular-protein-has-an-extracellular-role-as-a-modulator-of-matrix-mineralization
#15
Amsaveni Ramachandran, Sriram Ravindran, Chun-Chieh Huang, Anne George
Transforming growth factor beta receptor II interacting protein 1 (TRIP-1), a predominantly intracellular protein is localized in the ECM of bone. TRIP-1 lacks a signal peptide, therefore, in this study, we provide evidence that intracellular TRIP-1 can be packaged and exported to the ECM via exosomes. Overexpression of TRIP-1 in MC3T3-E1 cells resulted in increased matrix mineralization during differentiation and knockdown resulted in reduced effects. In vivo function of TRIP-1 was studied by an implantation assay performed using TRIP-1 overexpressing and knockdown cells cultured in a 3-dimmensional scaffold...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27877865/three-dimensional-printed-macroporous-polylactic-acid-hydroxyapatite-composite-scaffolds-for-promoting-bone-formation-in-a-critical-size-rat-calvarial-defect-model
#16
Haifeng Zhang, Xiyuan Mao, Zijing Du, Wenbo Jiang, Xiuguo Han, Danyang Zhao, Dong Han, Qingfeng Li
We have explored the applicability of printed scaffold by comparing osteogenic ability and biodegradation property of three resorbable biomaterials. A polylactic acid/hydroxyapatite (PLA/HA) composite with a pore size of 500 μm and 60% porosity was fabricated by three-dimensional printing. Three-dimensional printed PLA/HA, β-tricalcium phosphate (β-TCP) and partially demineralized bone matrix (DBM) seeded with bone marrow stromal cells (BMSCs) were evaluated by cell adhesion, proliferation, alkaline phosphatase activity and osteogenic gene expression of osteopontin (OPN) and collagen type I (COL-1)...
2016: Science and Technology of Advanced Materials
https://www.readbyqxmd.com/read/27871616/a-sensitive-fluorescence-biosensor-for-alkaline-phosphatase-activity-based-on-the-cu-ii-dependent-dnazyme
#17
Mengmeng Zhao, Yajuan Guo, Lixu Wang, Fang Luo, Cuiying Lin, Zhenyu Lin, Guonan Chen
Alkaline phosphatase (ALP) plays an important role in phosphate metabolism processes; deviation from its normal level may indicate different kinds of diseases, so it is highly necessary to develop some simple and sensitive methods to monitor the ALP level. In this study, a simple, high selective, and sensitive fluorescent biosensor has been proposed for ALP activity determination. The Cu(II)-dependent DNAzyme (Cu-Enzyme) are divided into two parts: Cu-Enzyme 1 and Cu-Enzyme 2, and labelled with alkyne and azido groups, respectively...
December 15, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27866252/identification-of-alkaline-phosphatase-genes-for-utilizing-a-flame-retardant-tris-2-chloroethyl-phosphate-in-sphingobium-sp-strain-tcm1
#18
Shouji Takahashi, Hiroshi Katanuma, Katsumasa Abe, Yoshio Kera
Tris(2-chloroethyl) phosphate (TCEP) is a haloalkyl phosphate flame retardant and plasticizer that has been recognized as a global environmental contaminant. Sphingobium sp. strain TCM1 can utilize TCEP as a phosphorus source. To identify the phosphomonoesterase involved in TCEP utilization, we identified four putative alkaline phosphatase (APase) genes, named SbphoA, SbphoD1, SbphoD2, and SbphoX-II, in the genome sequence. Following expression of these genes in Escherichia coli, APase activity was confirmed for the SbphoA and SbphoX-II gene products but was not clearly observed for the SbphoD1 and SbphoD2 gene products, owing to their accumulation in inclusion bodies...
November 19, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27858108/effects-of-ranitidine-antacid-food-and-formulation-on-the-pharmacokinetics-of-fostamatinib-results-from-five-phase-i-clinical-studies
#19
Talia Flanagan, Paul Martin, Michael Gillen, David Mathews, Eleanor Lisbon, Martin Kruusmägi
PURPOSE: Fostamatinib is an orally dosed phosphate prodrug that is cleaved by intestinal alkaline phosphatase to the active metabolite R406. Clinical studies were performed to assess the effect of food and ranitidine on exposure, to support in vitro-in vivo relationships (IVIVR) understanding and formulation transitions and to investigate absolute oral bioavailability. METHODS: A series of in vitro dissolution and clinical pharmacokinetic studies were performed to support the design and introduction of a new formulation, understand the impact of changes in in vitro dissolution on in vivo performance for two fostamatinib formulations, to characterize the effects of food and ranitidine on exposure, and determine the absolute oral bioavailability...
November 17, 2016: European Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/27854367/surface-organization-of-a-dppc-monolayer-on-concentrated-srcl2-and-zncl2-solutions
#20
Ellen M Adams, Dominique Verreault, Thilina Jayarathne, Richard E Cochran, Elizabeth A Stone, Heather C Allen
Transition metals are known to be enriched in organic-coated marine aerosols, but the impact these cations have on their surface properties is not well understood. Here the effect of Zn(2+) enrichment on the surface properties of a dipalmitoylphosphatidylcholine (DPPC) monolayer was investigated and compared to that of the alkaline earth metal Sr(2+), an ion not enriched in aerosols. Phase behavior of the DPPC film on concentrated aqueous solutions was probed with surface pressure-area isotherms while domain morphology was monitored with Brewster angle microscopy (BAM)...
November 30, 2016: Physical Chemistry Chemical Physics: PCCP
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