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alkaline phosphate

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https://www.readbyqxmd.com/read/28448526/human-alkaline-phosphatase-dephosphorylates-microbial-products-and-is-elevated-in-preterm-neonates-with-a-history-of-late-onset-sepsis
#1
Matthew Pettengill, Juan D Matute, Megan Tresenriter, Julie Hibbert, David Burgner, Peter Richmond, José Luis Millán, Al Ozonoff, Tobias Strunk, Andrew Currie, Ofer Levy
BACKGROUND: A host defense function for Alkaline phosphatases (ALPs) is suggested by the contribution of intestinal ALP to detoxifying bacterial lipopolysaccharide (endotoxin) in animal models in vivo and the elevation of ALP activity following treatment of human cells with inflammatory stimuli in vitro. However the activity of ALP in human plasma (primarily tissue-nonspecific ALP; TNAP) on lipopolysaccharide and other microbial products has not been assessed, nor has its expression been studied in preterm newborns, a vulnerable population at high risk of sepsis...
2017: PloS One
https://www.readbyqxmd.com/read/28447517/hepatoprotective-potential-of-phyllanthus-muellarianus-leaf-extract-studies-on-hepatic-oxidative-stress-and-inflammatory-biomarkers
#2
Taofeek O Ajiboye, Fatimah M Ahmad, Airat O Daisi, Aminat A Yahaya, Oluwayemisi B Ibitoye, Hamdalat F Muritala, Taofik O Sunmonu
CONTEXT: Leaves of Phyllanthus muellarianus (Kuntze) Exell. (Euphorbiacea) are widely used in the management of liver disorders in Nigeria. However, no there is no scientific validation to support this use. OBJECTIVE: Hepatoprotective effect of Phyllanthus muellarianus aqueous leaf extract was investigated in acetaminophen-induced liver injury mice. MATERIALS AND METHODS: Hepatoprotective effect of Phyllanthus muellarianus aqueous leaf extract was evaluated in acetaminophen-induced hepatic damage in Swiss albino mice using biomarkers of hepatocellular indices, oxidative stress, proinflammatory factors and lipid peroxidation...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28441338/effects-of-3d-printed-polycaprolactone-%C3%AE-tricalcium-phosphate-membranes-on-guided-bone-regeneration
#3
Jin-Hyung Shim, Joo-Yun Won, Jung-Hyung Park, Ji-Hyeon Bae, Geunseon Ahn, Chang-Hwan Kim, Dong-Hyuk Lim, Dong-Woo Cho, Won-Soo Yun, Eun-Bin Bae, Chang-Mo Jeong, Jung-Bo Huh
This study was conducted to compare 3D-printed polycaprolactone (PCL) and polycaprolactone/β-tricalcium phosphate (PCL/β-TCP) membranes with a conventional commercial collagen membrane in terms of their abilities to facilitate guided bone regeneration (GBR). Fabricated membranes were tested for dry and wet mechanical properties. Fibroblasts and preosteoblasts were seeded into the membranes and rates and patterns of proliferation were analyzed using a kit-8 assay and by scanning electron microscopy. Osteogenic differentiation was verified by alizarin red S and alkaline phosphatase (ALP) staining...
April 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28440742/leptin-overexpression-in-bone-marrow-stromal-cells-promotes-periodontal-regeneration-in-a-rat-model-of-osteoporosis
#4
Baoyu Zheng, Jun Jiang, Yuling Chen, Minkui Lin, Zhibin Du, Yin Xiao, Kai Luo, Fuhua Yan
BACKGROUND: Osteoporosis (OP) is associated with widespread periodontitis and impaired periodontal healing. However, there is a lack of information about the outcomes of regenerative approaches under the influence of OP. This study investigated the effect of leptin (LEP) overexpression on the regenerative potential of bone marrow stromal cells (BMSCs) in an osteoporotic rat periodontal fenestration defect model. METHODS: Rat BMSCs were transfected with adenoviruses harboring the human (h) LEP gene...
April 25, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28440703/zeta-potential-changing-nanoparticles-conjugated-with-cell-penetrating-peptides-for-enhanced-transfection-efficiency
#5
Wongsakorn Suchaoin, Arshad Mahmood, Kesinee Netsomboon, Andreas Bernkop-Schnürch
AIM: The aim of this study was to develop zeta-potential-changing nanoparticles (NPs) combining cell-penetrating peptides for gene delivery. METHODS & MATERIALS: NPs were formed using phosphorylated carboxymethyl cellulose-glucosamine 6-phosphate (CMC-G6P) and polyethylene imine-polyarginine conjugates. Phosphate release was evaluated using intestinal alkaline phosphatase and cell lines. Transfection studies with plasmid DNA were then performed. RESULTS: The zeta potential of CMC-G6P/branched PEI NPs was -3 mV and switched to +4 mV after intestinal alkaline phosphatase cleavage...
April 25, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28435849/effects-of-cyclic-compression-on-the-mechanical-properties-and-calcification-process-of-immature-chick-bone-tissue-in-culture
#6
Eijiro Maeda, Masashi Nakagaki, Katsuhisa Ichikawa, Kazuaki Nagayama, Takeo Matsumoto
Contribution of mechanical loading to tissue growth during both the development and post-natal maturation is of a particular interest, as its understanding would be important to strategies in bone tissue engineering and regenerative medicine. The present study has been performed to investigate how immature bone responds to mechanical loading using an ex vivo culture system. A slice of the tibia, with the thickness of 3 mm, was obtained from 0-day-old chick. For the ex vivo culture experiment in conjunction with cyclic compressive loading, we developed a custom-made, bioreactor system where both the load and the deformation applied to the specimen was recorded...
June 2017: Bone Reports
https://www.readbyqxmd.com/read/28435036/costunolide-increases-osteoblast-differentiation-via-atf4-dependent-ho-1-expression-in-c3h10t1-2-cells
#7
Wan-Jin Jeon, Kyeong-Min Kim, Eun-Jung Kim, Won-Gu Jang
AIMS: Costunolide is a sesquiterpene lactones used in many herbal medicines, with well-established anti-inflammatory and anti-oxidant functions modulating endoplasmic reticulum (ER) stress pathways, and which promotes the expression of anti-oxidant genes. The aim of this study is to investigate whether costunolide is involved in osteoblast differentiation and, determine the mechanisms of differentiation in mesenchymal stem cells. MAIN METHODS: The cytotoxicity of costunolide was identified using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay...
April 20, 2017: Life Sciences
https://www.readbyqxmd.com/read/28431438/comparison-of-intact-parathyroid-hormone-alkaline-phosphatase-phosphate-levels-for-diagnosing-severe-metabolic-bone-disease-in-infants-with-severe-bronchopulmonary-dysplasia
#8
Erin K Tkach, Ammie M White, Kevin C Dysart, Brenda Waber, Ursula S Nawab, Huayan Zhang, Erik A Jensen
No abstract text is available yet for this article.
April 21, 2017: American Journal of Perinatology
https://www.readbyqxmd.com/read/28429838/pyrophosphate-as-substrate-for-alkaline-phosphatase-activity-a-convenient-flow-injection-chemiluminescence-assay
#9
Qingfeng Zhang, Cuiyun Zhang, Meiding Yang, Donghong Yu, Cong Yu
A sensitive and convenient flow-injection chemiluminescence (FI-CL) turn-on assay for alkaline phosphatase (ALP) activity without any label and synthesis is developed. Cu(2+) can catalyze the luminol-H2 O2 CL reaction. Pyrophosphate (PPi) can chelate Cu(2+) and therefore the Cu(2+) -mediated luminol-H2 O2 CL reaction is inhibited. The addition of ALP can catalyze the hydrolysis of PPi into phosphate ions, Cu(2+) is released and the chemiluminescence recovers. A detection limit of 1 mU/mL ALP is obtained.
April 21, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28427668/synthesis-of-hydroxyapatite-nanorods-for-application-in-water-defluoridation-and-optimization-of-process-variables-advantage-of-ultrasonication-with-precipitation-method-over-conventional-method
#10
Dhiraj Mehta, Poonam Mondal, Virendra Kumar Saharan, Suja George
This research work presents the synthesis of hydroxyapatite (Hap) nanorods for defluoridation of drinking water by using both conventional (CM) and ultrasonication with precipitation (USPM) methods. Calcium nitrate was reacted with potassium phosphate in presence of ammonia for controlled pH to synthesize Hap nanorods, which was characterized using FTIR, XRD, SEM, TG-DTA, and TEM/EDS for determining its phase composition, structural and thermal decomposition behavior. When USPM method was used for synthesis, the yield of the Hap nanorods was improved from 83...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28425557/dual-spectra-band-emissive-eu-2-mn-2-co-activated-alkaline-earth-phosphates-for-indoor-plant-growth-novel-phosphor-converted-leds
#11
Young Jun Yun, Jin Kyu Kim, Ji Young Ju, Seul Ki Choi, Woon Ik Park, Jae Yong Suh, Ha-Kyun Jung, Yongseon Kim, Sungho Choi
This paper reports designing a novel single composition blue/red color illuminating phosphor followed by fabricating "smart" agricultural/horticultural LED lighting. Color-tunable Eu(2+)/Mn(2+) co-activated alkaline earth phosphates, Na(Sr,Ba)PO4 and Ca3Mg3(PO4)4, are considered, and the stable doping sites for the corresponding activators are identified by using first-principle DFT calculations. We can realize the designated color purity with stable thermal quenching preserved luminescence behavior is induced by the Eu(2+) center positioned at different coordination states with intermixed Sr(2+)/Ba(2+) sites in Na(Sr,Ba)PO4 hosts...
April 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28423307/aspects-of-phosphorus-physiology-associated-with-phosphate-induced-polar-lipid-remodelling-in-marine-microalgae
#12
José Pedro Cañavate, Isabel Armada, Ismael Hachero-Cruzado
Marine microalgae exhibit a diversified phosphorus physiology and have also been recently found to show high inter-taxa variability in their phosphate induced-polar lipids' remodelling. Identification of phosphorus physiology aspects that are more related to lipid remodelling can contribute to better understanding of such intricate phytoplankton lipid metabolism. Therefore, some aspects of phosphorus physiology related to its uptake, storage and use were evaluated in a taxonomically diversified group of nine marine microalgae that was arranged into three subgroups, each of them including species showing similar polar lipid responses to phosphate...
March 31, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28414662/weil-s-disease-from-a-local-new-orleans-bar
#13
H P Kahn, L Bateman
INTRODUCTION: Leptospirosis is a zoonotic infection that typically presents with fever, myalgias, nausea, and vomiting after contact with contaminated waters or infected animals (typically rodents); and their excrements. Conditions favorable to the transmission of leptospirosis are common in LA and, without treatment, leptospirosis can lead to both liver and renal failure, meningitis, pulmonary hemorrhage and ultimately death. CASE: A 56 year old woman with no past medical history presented to the Emergency Department with weakness, myalgias, jaundice and decreased urine output for one week...
March 2017: Journal of the Louisiana State Medical Society: Official Organ of the Louisiana State Medical Society
https://www.readbyqxmd.com/read/28411823/nucleic-acid-controlled-quantum-dots-aggregation-a-label-free-fluorescence-turn-on-strategy-for-alkaline-phosphatase-detection
#14
Zhenzhen Hu, Jian Chen, Yongxin Li, Yan Wang, Qingfeng Zhang, Ejaz Hussain, Meiding Yang, Sohail Anjum Shahzad, Donghong Yu, Cong Yu
Based on the controlled aggregation of quantum dots (QDs), a valid, reliable, and label-free fluorescence turn-on strategy is established for the detection of alkaline phosphatase activity. The aqueous solution of anionic QDs exhibits intense fluorescence. However, the addition of cationic polymer (poly-1) significantly quenched the fluorescence of the QDs via their induced aggregation. While short 3'-phosphorylated DNA (DNA-P) could not be extended by terminal deoxynucleotidyl transferase (TdT) and therefore, fluorescence of the QDs was recovered negligibly...
July 1, 2017: Talanta
https://www.readbyqxmd.com/read/28410527/mechanisms-of-the-photochemical-release-of-phosphate-from-resuspended-sediments-under-solar-irradiation
#15
Xiaolu Li, Yiyong Zhou, Guanglong Liu, Hongjun Lei, Duanwei Zhu
In previous studies, resuspended sediments that were exposed to simulated solar irradiation could release dissolved phosphate (PO4(3-)). However, the mechanisms of phosphate release remain unclear. In this research, a battery of experiments was performed to reveal the mechanisms of the photochemical release of phosphate from resuspended sediments of a shallow eutrophic lake under solar irradiation. The results show that the PO4(3-) released in resuspended sediments was significantly higher than that in the dark control or in water alone after treatment with solar irradiation for 6h...
April 11, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28401263/mutational-and-biochemical-findings-in-adults-with-persistent-hypophosphatasemia
#16
F E McKiernan, J Dong, R L Berg, E Scotty, P Mundt, L Larson, I Rai
A majority of adults with persistently low serum alkaline phosphatase values carry a pathogenic or likely pathogenic variant in the ALPL gene and also have elevated alkaline phosphatase substrate values in serum and urine. These adults may fall within the spectrum of the adult form of hypophosphatasia. INTRODUCTION: The primary objective of this study was to determine what proportion of adults with persistently low serum alkaline phosphatase values (hypophosphatasemia) harbor mutations in the ALPL gene or have elevated alkaline phosphatase (ALP) substrates...
April 12, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/28400821/the-great-impostor-transaminitis-masking-the-coinfection-of-syphilis-and-human-immunodeficiency-virus
#17
Sunit Tolia, Hassan Kassem, Ana Capatina-Rata
Introduction. The incidence of syphilis continues to rise in the United States over the past 15 years. This disease process is classified into stages and may present with a coinfection of Human Immunodeficiency Virus (HIV). Case Report. We present a case of a 32-year-old African American male who presented with cutaneous manifestations of secondary syphilis and transaminitis. A workup revealed that the transaminitis was secondary to underlying syphilitic hepatitis in the presence of HIV coinfection. The patient had a reactive rapid plasma reagin (RPR) of 1 : 64 TU and reactive Treponema pallidum particle agglutination assay (TPPA)...
2017: Case Reports in Medicine
https://www.readbyqxmd.com/read/28399445/sensitive-detection-of-alkaline-phosphatase-by-switching-on-gold-nanoclusters-fluorescence-quenched-by-pyridoxal-phosphate
#18
Mohamed Ibrahim Halawa, Wenyue Gao, Muhammad Saqib, Shimeles Addisu Kitte, Fengxia Wu, Guobao Xu
In this work, we designed highly sensitive and selective luminescent detection method for alkaline phosphatase using bovine serum albumin functionalized gold nanoclusters (BSA-AuNCs) as the nanosensor probe and pyridoxal phosphate as the substrate of alkaline phosphatase. We found that pyridoxal phosphate can quench the fluorescence of BSA-AuNCs and pyridoxal has little effect on the fluorescence of BSA-AuNCs. The proposed mechanism of fluorescence quenching by PLP was explored on the basis of data obtained from high-resolution transmission electron microscopy (HRTEM), dynamic light scattering (DLS), UV-vis spectrophotometry, fluorescence spectroscopy, fluorescence decay time measurements and circular dichroism (CD) spectroscopy...
April 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28386818/potential-risk-factors-for-vitamin-d-levels-in-medium-and-long-term-use-of-antiepileptic-drugs-in-childhood
#19
Edibe Pempegül Yildiz, Şükran Poyrazoglu, Gonca Bektas, Aslı Derya Kardelen, Nur Aydinli
Antiepileptic drugs (AED) have potential side effects through vitamin-D. Prevalence of vitamin D insufficiency and potential risk factors for the longitudinal changes of vitamin D levels compared to its baseline levels under AED treatment were investigated in this study. This retrospective study includes patients whose AED therapy were started in only autumn months, between 2000 and 2014. Detailed assessment of neurologic diagnosis and brain MRI findings, ambulatory status, types and durations of AED treatment, and baseline bone health blood tests (vitamin-D, alkaline phosphatase, calcium, and phosphate levels) were obtained on all patients...
April 6, 2017: Acta Neurologica Belgica
https://www.readbyqxmd.com/read/28380671/bicomponent-fibrous-scaffolds-made-through-dual-source-dual-power-electrospinning-dual-delivery-of-rhbmp-2-and-ca-p-nanoparticles-and-enhanced-biological-performances
#20
Chong Wang, William Weijia Lu, Min Wang
Electrospun scaffolds incorporated with both calcium phosphates (Ca-P) and bone morphogenetic protein-2 (BMP-2) have been used for bone tissue regeneration. However, in most cases BMP-2 and Ca-P were simply mixed and loaded in a monolithic structure, risking low BMP-2 loading level, reduced BMP-2 biological activity, uncontrolled BMP-2 release and inhomogeneous Ca-P distribution. In this investigation, novel bicomponent scaffolds having evenly distributed rhBMP-2-containing fibers and Ca-P nanoparticle-containing fibers were made using an established dual-source dual-power electrospinning technique with the assistance of emulsion electrospinning and blend electrospinning...
April 5, 2017: Journal of Biomedical Materials Research. Part A
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