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alkaline phosphate

He Wang, Jianjia Li, Xiaoyi Zhang, Tingting Ning, Dandan Ma, Yihong Ge, Shuaimei Xu, Yilin Hao, Buling Wu
To seek a potential target for periodontal tissue regeneration, this study aimed to explore the role of Integrin alpha 5 (ITGA5) in human periodontal ligament stem cells (PDLSCs). Transwell assay, Cell Counting Kit 8 (CCK8) assay, cell cycle assay, alkaline phosphatase (ALP) activity, alizarin red staining, and western blot were used to investigate the effects of ITGA5 on PDLSC migration, proliferation and osteogenic differentiation. The in vivo effect was investigated by nude mice subcutaneous transplantation with cell and hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) complex...
March 12, 2018: Journal of Proteomics
Evgeniya A Malykh, Ivan A Butov, Anna B Ravcheeva, Alexander A Krylov, Sergey V Mashko, Nataliya V Stoynova
BACKGROUND: In the L-histidine (His) biosynthetic pathway of Escherichia coli, the first key enzyme, ATP-phosphoribosyltransferase (ATP-PRT, HisG), is subject to different types of inhibition. Eliminating the feedback inhibition of HisG by the His end product is an important step that enables the oversynthesis of His in breeding strains. However, the previously reported feedback inhibition-resistant mutant enzyme from E. coli, HisGE271K , is inhibited by purine nucleotides, particularly ADP and AMP, via competitive inhibition with its ATP substrate...
March 15, 2018: Microbial Cell Factories
Irena Branyikova, Monika Filipenska, Katerina Urbanova, Marek C Ruzicka, Martin Pivokonsky, Tomas Branyik
Alkaline flocculation has been studied due to its potential as a low-cost harvesting method for microalgae. However, surface properties (zeta potential, contact angles) as inputs into physicochemical interaction models have not yet been applied systematically. In this work, forced alkaline flocculation of the freshwater microalgae Chlorella vulgaris induced by calcium phosphate precipitates was studied as a model system. Response surface methodology was used to quantify the effect of independent variables (concentration of Ca2+ (0...
March 6, 2018: Colloids and Surfaces. B, Biointerfaces
Hyun-Soo Kim, Mingzhen Zheng, Do-Kyung Kim, Won-Pyo Lee, Sang-Joun Yu, Byung-Ock Kim
Purpose: The purpose of this study was to evaluate the effects of 1,25-dihydroxyvitamin D3 on the proliferation, differentiation, and matrix mineralization of MC3T3-E1 osteoblast-like cells in vitro. Methods: MC3T3-E1 osteoblastic cells and 1,25-dihydroxyvitamin D3 were prepared. Cytotoxic effects and osteogenic differentiation were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, alkaline phosphatase (ALP) activity assay, ALP staining, alizarin red S staining, and reverse transcription-polymerase chain reaction (RT-PCR) for osteogenic differentiation markers such as ALP, collagen type I (Col-I), osteocalcin (OCN), vitamin D receptor (VDR), and glyceraldehyde 3-phosphate dehydrogenase...
February 2018: Journal of Periodontal & Implant Science
Minhui He, Qiong Hu, Yaqi Mei, Baojing Zhou, Jinming Kong, Xueji Zhang
The ability to directly detect alkaline phosphatase (ALP) activity in undiluted serum samples is of great importance for clinical diagnosis. In this work, we report the use of the distinctive metal-to-ligand charge-transfer (MLCT) absorption properties of the Cu(BCA)2 + (BCA = bicinchoninic acid) reporter for the visual detection of ALP activity. In the presence of ALP, the substrate ascorbic acid 2-phosphate (AAP) can be enzymatically hydrolyzed to release ascorbic acid (AA), which in turn reduces Cu2+ to Cu+ ...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Islam Amine Larabi, Marie Martin, Isabelle Etting, Pauline Penot, Nicolas Fabresse, Jean Claude Alvarez
Recently, the emergence of new psychoactive substances (NPS) has led to their wide use among clubbers and men who have sex with men (MSM) for their stimulant effects. However, their use in drug facilitated sexual assault (DFSA) has rarely been described. Herein we report a case of a 44 years-old man who was assaulted after a party. Due to late reporting of the offense, only hair (black) was sampled 15 days later and a segmental analysis was achieved to look for most DFSA agents and NPS. Twenty mg of each segment (A:0-1cm, B:1-3cm and C:3-5cm) were incubated in phosphate buffer pH 5...
March 10, 2018: Drug Testing and Analysis
Yu-Ting Gong, Fang Yuan, Yuming Dong, Zaijun Li, Guang-Li Wang
A novel and general strategy of split-type immunoassay is developed based on redox chemical reaction modulated photoelectrochemistry of carbon dots (CDs). Specifically, the photocurrent of the CDs sensitized titanium dioxide nanoparticles (TiO2 NPs) modified fluorine doped indium tin oxide (FTO) (that is the FTO/TiO2 /CDs) electrode was inhibited obviously by KMnO4 due to the oxidation of surface hydroxyl groups of CDs to electron accepting carbonyls. While the inhibited photocurrent of the KMnO4 treated FTO/TiO2 /CDs electrode can be restored by ascorbic acid (AA) because of the regeneration of electron donating hydroxyls to promote electron-hole separation...
July 19, 2018: Analytica Chimica Acta
Baltzar Stevensson, Yang Yu, Mattias Edén
We present a comprehensive molecular dynamics (MD) simulation study of composition-structure trends in a set of 25 glasses of widely spanning compositions from the following four systems of increasing complexity: Na2 O-B2 O3 , Na2 O-B2 O3 -SiO2 , Na2 O-CaO-SiO2 -P2 O5 , and Na2 O-CaO-B2 O3 -SiO2 -P2 O5 . The simulations involved new B-O and P-O potential parameters developed within the polarizable shell-model framework, thereby combining the beneficial features of an overall high accuracy and excellent transferability among different glass systems and compositions: this was confirmed by the good accordance with experimental data on the relative BO3 /BO4 populations in borate and boro(phospho)silicate networks, as well as with the orthophosphate fractions in bioactive (boro)phosphosilicate glasses, which is believed to strongly influence their bone-bonding properties...
March 9, 2018: Physical Chemistry Chemical Physics: PCCP
Yingzhi Gu, Yuxing Bai, Dongliang Zhang
The aim of the present study was to construct and compare gelatin-HA-TCP scaffolds with a gelatin-only scaffold and to investigate the effect of the scaffold on osteogenic differentiation of human dental pulp stem cells. We developed a novel scaffold for bone tissue engineering via a solution casting/particle washing method, and the physical and mechanical properties of the scaffolds were examined using scanning electron microscopy and a universal testing machine, respectively. Scaffold cytotoxicity toward hDPSCs was evaluated with the CCK8 method, and hDPSC differentiation was evaluated with an alkaline phosphatase activity assay, alizarin red S staining, and real-time PCR...
March 9, 2018: Journal of Biomedical Materials Research. Part A
Muhammad Adnan, Zahir Shah, Shah Fahad, Muhamamd Arif, Mukhtar Alam, Imtiaz Ali Khan, Ishaq Ahmad Mian, Abdul Basir, Hidayat Ullah, Muhammad Arshad, Inayat-Ur Rahman, Shah Saud, Muhammad Zahid Ihsan, Yousaf Jamal, Amanullah, Hafiz Mohkum Hammad, Wajid Nasim
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
March 7, 2018: Scientific Reports
Primploy Greeviroj, Thidarat Kitrungphaiboon, Pisut Katavetin, Kearkiat Praditpornsilpa, Somchai Eiam-Ong, Bertrand L Jaber, Paweena Susantitaphong
BACKGROUND: Cinacalcet could decrease serum calcium, phosphate, and parathyroid hormone (PTH) in previous meta-analyses. However, the effect of cinacalcet on the new biomarkers such as fibroblast growth factor-23 (FGF-23), bone markers, and vascular calcification are still unestablished. We conducted a meta-analysis to examine the effects of cinacalcet on all laboratory and clinical spectrums of chronic kidney disease-mineral bone disorders (CKD-MBD). METHODS: A systematic literature search was conducted in MEDLINE, Scopus, Cochrane Central Register of Controlled Trials, and Clinical Trials...
March 7, 2018: Nephron
Mengqun Yao, Yinchu Ma, Hang Liu, Malik Ihsanullah Khan, Song Shen, Shuya Li, Yangyang Zhao, Yi Liu, Guoqing Zhang, Xiaoqiu Li, Fei Zhong, Wei Jiang, Yucai Wang
Multidrug resistance (MDR) is the major cause for chemotherapy failure, which constitutes a formidable challenge in the field of cancer therapy. The synergistic chemo-photothermal treatment has been reported to be a potential strategy to overcome MDR. In this work, rationally designed enzyme-degradable, hyperbranched polyphosphoester nanomedicines were developed for reversing MDR via the co-delivery of doxorubicin and IR-780 (hPPEDOX&IR) as combined chemo-photothermal therapy. The amphiphilic hyperbranched polyphosphoesters with phosphate bond as the branching point were synthesized via a simple but robust one-step polycondensation reaction...
March 7, 2018: Biomacromolecules
Chris Steffi, Dong Wang, Chee Hoe Kong, Zuyong Wang, Poon Nian Lim, Zhilong Shi, Eng San Thian, Wilson Wang
Estrogen, a steroid hormone, plays an important role in modulating osteoclast proliferation and development. Estrogen deficiency boosts osteoclast activity, leading to osteoporosis in elderly women. In this study, 17-ß estradiol (E2) loaded poly(ε-caprolactone) (PCL)/silk fibroin (SF) electrospun microfibers was developed as a proposed localized E2 delivery system to treat osteoporotic fractures. PCL is a synthetic polymer known for its biocompatibility and excellent mechanical properties. The bioactivity of PCL was enhanced by mixing it with natural silk fibroin (SF) polymer that has low immunogenicity and inherent bioactivity...
March 7, 2018: ACS Applied Materials & Interfaces
Ana Paula C A Beltrame, Dakota Suchyta, Islam Abd Alraheam, Anas Mohammed, Mark Schoenfisch, Ricardo Walter, Izabel C S Almeida, Liliete C Souza, Patricia A Miguez
The aim of this study was to evaluate the antierosive effect of phosphorylated chitosan in dentin. Bovine dentin specimens were randomly distributed into the following groups: (1) no treatment (NoTx/negative control), (2) phosphate-buffered saline solution (PBS), (3) AmF/NaF/SnCl2 (positive control), (4) 0.5% chitosan solution (Chi), (5) 0.5% neutral phosphorylated (NP)-Chi, and (6) 0.5% alkaline phosphorylated (AP)-Chi. The specimens were submitted to de-remineralization treatment cycles for 5 days: 0.5% citric acid (2 min), remineralizing solution (30 min), and surface treatment according to assigned groups (2 min, 6×/day)...
March 6, 2018: Caries Research
Uche Godfrey Okeke, Deniz Akdemir, Ismail Rabbi, Peter Kulakow, Jean-Luc Jannink
The HarvestPlus program for cassava ( Crantz) fortifies cassava with β-carotene by breeding for carotene-rich tubers (yellow cassava). However, a negative correlation between yellowness and dry matter (DM) content has been identified. We investigated the genetic control of DM in white and yellow cassava. We used regional heritability mapping (RHM) to associate DM with genomic segments in both subpopulations. Significant segments were subjected to candidate gene analysis and candidates were validated with prediction accuracies...
March 2018: Plant Genome
Alexandra Whicher, Eloi Camprubi, Silvana Pinna, Barry Herschy, Nick Lane
Metabolism is primed through the formation of thioesters via acetyl CoA and the phosphorylation of substrates by ATP. Prebiotic equivalents such as methyl thioacetate and acetyl phosphate have been proposed to catalyse analogous reactions at the origin of life, but their propensity to hydrolyse challenges this view. Here we show that acetyl phosphate (AcP) can be synthesised in water within minutes from thioacetate (but not methyl thioacetate) under ambient conditions. AcP is stable over hours, depending on temperature, pH and cation content, giving it an ideal poise between stability and reactivity...
March 3, 2018: Origins of Life and Evolution of the Biosphere
Israel Ikoyi, Andrew Fowler, Achim Schmalenberger
This study evaluated the effect of one-time phosphate fertilization on the soil microbiota, its cycling of phosphorus (P) and grass growth. Soil columns were established in a greenhouse using a P-limited Irish soil (index 1), planted with Lolium perenne and fertilized with 0 (control), 5 (quarter), 10 (half) and 20 (full)kgPha-1 as inorganic phosphate. Only traces of phosphate in soil solution were detected over the 14week experiment, even after phosphate fertilization. Grass dry matter yield between treatments was not significantly different...
February 27, 2018: Science of the Total Environment
M Hermassi, J Dosta, C Valderrama, E Licon, N Moreno, X Querol, N H Batis, J L Cortina
The use of low-cost inorganic sorbents as a new sustainable strategy to enhance the valorization of nutrients (N-P-K), from the urban water cycle (e.g., side streams from sewage sludge anaerobic digestion), in agriculture applications is presented. The simultaneous recovery and stabilization of ammonium and phosphate by using a mixture of two reactive sorbents (Na and K zeolites and magnesium oxide) was evaluated. The nutrients stabilization process, favoured at alkaline pH values, is carried out by a) the precipitation of phosphate ions with magnesium and/or ammonium ions and b) the sorption of ammonium by Na- and K-zeolites...
February 27, 2018: Science of the Total Environment
Musa O Babalola, Ngozi A Imaga, Titilola A Samuel, Iyanu P Diriwari, Olajumoke Kolade, Irene Ezeamalu, Adefioye O Laoye, Oyesola O Ojewunmi
Glucose-6-phosphate dehydrogenase (G6PD) is an essential enzyme in the pentose phosphate pathway that prevents oxidative damage to cells. This study determined the genotypic and allelic frequencies of G6PD G202A and A376G and also investigated correlation between G6PD polymorphisms and hemoglobin (Hb) phenotypes in children in Lagos, Nigeria. Seventy-eight children [55 with Hb AA (βΑ /βA ) and 23 with Hb AS (βΑ /βS ) trait] and 65 Hb SS (βS /βS ) (HBB: c.20A>T) subjects in steady state with age range between 5-15 years were recruited for the study...
March 1, 2018: Hemoglobin
Wenbo Liu, Nora B Sutton, Huub H M Rijnaarts, Alette A M Langenhoff
This is the first study examining pharmaceutical removal under anoxic conditions with MnO2 . This study compares the abiotic removal of seven pharmaceuticals with reactive MnO2 particles in the presence of oxygen (oxic conditions) and in the absence of oxygen (anoxic conditions). Due to the novelty of pharmaceutical removal under anoxic conditions, the influence of phosphate buffer, pH, and MnO2 morphologies is also examined. Results show that over 90% of diclofenac is removed under anoxic conditions. Additionally, we found that (1) anoxic conditions are beneficial for diclofenac removal with MnO2 , (2) phosphate buffer affects the pharmaceutical removal efficiencies, (3) higher pharmaceutical removal is obtained at acidic pH compared to that at neutral or alkaline conditions, and (4) amorphous MnO2 removes pharmaceuticals better than crystalline MnO2 ...
February 28, 2018: Environmental Science and Pollution Research International
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