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alkaline phosphate

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https://www.readbyqxmd.com/read/28225266/enhanced-phosphorus-locking-by-novel-lanthanum-aluminum-hydroxide-composite-implication-for-eutrophication-control
#1
Rui Xu, Meiyi Zhang, Robert J G Mortimer, Gang Pan
Lanthanum (La) bearing materials have been widely used to remove phosphorus (P) in water treatment. However, it remains a challenge to enhance phosphate (PO4) adsorption capacity and La usage efficiency. In this study, La was co-precipitated with aluminum (Al) to obtain a La/Al-hydroxide composite (LAH) for P adsorption. The maximum PO4 adsorption capacities of LAH (5.3% La) were 76.3 and 45.3 mg P g-1 at pH 4.0 and 8.5, which were 8.5 and 5.3 times higher than those of commercially available La-modified bentonite (Phoslock®, 5...
February 22, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28222375/on-site-nutrient-recovery-and-removal-from-source-separated-urine-by-phosphorus-precipitation-and-short-cut-nitrification-denitrification
#2
Song Yao, Liping Chen, Detian Guan, Zhongguo Zhang, Xiujun Tian, Aimin Wang, Guotian Wang, Qian Yao, Dangcong Peng, Jiuyi Li
Source separation and treatment of human urine have been recognized as a resource-efficient alternative to conventional urban drainage, not only reducing nutrient loads on municipal wastewater treatment plants, but recovering valuable resources from waste streams. In this work, on-site phosphorus (P) recovery from real urine was carried out by using the brine from a reverse osmosis process as the flush water for urine-diverting toilets and a P precipitant, while nitrogen (N) was removed via short-cut nitrification-denitrification (SCND) in a membrane bioreactor (MBR)...
February 16, 2017: Chemosphere
https://www.readbyqxmd.com/read/28220699/quantum-mechanical-study-on-the-catalytic-mechanism-of-alkaline-phosphatases
#3
Gabriela L Borosky
Alkaline phosphatases (APs) catalyze the hydrolysis and transphosphorylation of phosphate monoesters. The catalytic mechanism was examined by quantum-mechanical calculations using an active-site model based on the X-ray crystal structure of the human placental AP. Free energies of activation and of reaction for the catalytic steps were evaluated for a series of aryl and alkyl phosphate esters, and the computational results were compared with experimental values available in the literature. Mechanistic observations previously reported in experimental works were rationalized by the present theoretical study, particularly regarding the difference in the rate-determining step between aryl and alkyl phosphates...
February 21, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28219249/enhanced-photoelectrochemical-method-for-sensitive-detection-of-protein-kinase-a-activity-using-tio2-g-c3n4-pamam-dendrimer-and-alkaline-phosphatase
#4
Xue Li, Lusheng Zhu, Yunlei Zhou, Huanshun Yin, Shiyun Ai
A novel photoelectrochemical (PEC) assay is developed for sensitive detection of protein kinase A (PKA) activity based on PKA-catalyzed phosphorylation reaction in solution and signal amplification strategy triggered by PAMAM dendrimer and alkaline phosphatase (ALP). In this strategy, it is noteworthy at this point that PKA phosphorylation was achieved in solution instead of on the surface of the electrode, which has advantages of the good contact in reactants and simple experimental procedure. For immobilizing the phosphorylated peptide (P-peptide) on electrode surface, graphite-like carbon nitride (g-C3N4) and titanium dioxide (TiO2) complex is synthesized and characterized, which plays a significant role for TiO2 conjugating phosphate groups and g-C3N4 providing PEC signal...
February 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28218482/osteoblastic-differentiation-of-periodontal-ligament-stem-cells-on-non-stoichiometric-calcium-phosphate-and-titanium-surfaces
#5
L Winning, L Robinson, A R Boyd, I A El Karim, F T Lundy, B J Meenan
Bioactive materials offer particular clinical benefits in the field of dental implantology, where differentiation of stem cells towards an osteoblastic lineage is required for osseointegration and appropriate function of implants in vivo. The aim of this study was to evaluate the osteoblastic response of Stro-1+ve periodontal ligament stem cells (PDLSCs) to three well-characterised biomaterial surfaces: an abraded titanium surface (cpTi) control; a polycrystalline titanium surface, with both micro and nano-topography produced by radio frequency magnetron sputtering (TiTi); and the same surface incorporating a sputter deposited calcium phosphate coating (CaP-TiTi)...
February 20, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28214622/preferable-uptake-of-phosphate-by-hydrous-zirconium-oxide-nanoparticles-embedded-in-quaternary-ammonium-chinese-reed
#6
Yanan Shang, Xing Xu, Shuto Qi, Yanxia Zhao, Zhongfei Ren, Baoyu Gao
Phosphate capture from aqueous was conducted using hydrous zirconium oxide (HZO) embedded in quaternary-ammonium Chinese reed (CR-N(+)-HZO), and the characteristics of adsorbent was determined. HZO was dispersed as nanoparticles or nano-clusters on the external or inside the networking pores of CR-N(+)-HZO. The surface of CR-N(+)-HZO was heterogeneous with multiple adsorption sites, HZO nanocomposite and N(+)(CH2CH3)3Cl(-), which both contributed to the adsorption process. The phosphate uptake by CR-N(+)-HZO was optimal at pH 3...
February 12, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28212004/fluorescence-regulation-of-poly-thymine-templated-copper-nanoparticles-via-an-enzyme-triggered-reaction-towards-sensitive-and-selective-detection-of-alkaline-phosphatase
#7
Junyao Li, Ling Si, Jianchun Bao, Zhaoyin Wang, Zhihui Dai
The activity of alkaline phosphatase (ALP) is a crucial index of blood routine examinations, since the concentration of ALP is highly associated with various human diseases. To address the demands of clinical tests, efforts should be made to develop more approaches that can sense ALP in real samples. Recently, we find that fluorescence of poly(30T)-templated copper nanoparticles (CuNPs) can be directly and effectively quenched by pyrophosphate ion (PPi), provid-ing new perspective in designing sensitive biosensors based on DNA-templated CuNPs...
February 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28208287/highly-uniform-gold-nanobipyramids-for-ultrasensitive-colorimetric-detection-of-influenza-virus
#8
Shaohua Xu, Wenjun Ouyang, Peisi Xie, Yi Lin, Bin Qiu, Zhenyu Lin, Guonan Chen, Longhua Guo
Gold nanoparticles (AuNPs) have been frequently utilized for the construction of diverse colorimetric biosensors. Normally, AuNPs with sharp edges could have better sensitivity. However, the poor monodipersity of AuNPs with sharp edges seriously confines their utility for colorimetric biosensing. Herein, we demonstrate the utility of highly uniform gold nanobipyramids (Au NBPs) for ultrasensitive colorimetric detection of H5N1 virus. The proposed method is based on the fact that alkaline phosphatase (ALP) could catalyze the decomposition of 4-aminophenyl phosphate (4-APP) to generate 4-aminophenol (4-AP), which would then reduce silver nitrate to metal silver and then deposited on Au NBPs...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28202997/-a-novel-tissue-engineered-bone-constructed-by-using-human-adipose-derived-stem-cells-and-biomimetic-calcium-phosphate-scaffold-coprecipitated-with-bone-morphogenetic-protein-2
#9
W R Jiang, X Zhang, Y S Liu, G Wu, Y J Ge, Y S Zhou
OBJECTIVE: To construct a novel biomimetic calcium phosphate (BioCaP) scaffold loaded with bone morphogenetic protein-2 (BMP-2), and to investigate its role in the osteogenesis of human adipose-derived stem cells (hASCs) in vitro and in vivo. METHODS: The BioCaP scaffold coprecipitated with BMP-2 (BMP-2-BioCaP) was constructed in this study. Field emission scanning electron microscopy (SEM) was used to analyze the morphology of the surfaces. The release kinetics was measured to evaluate the slow-release characteristics in vitro...
February 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28199823/an-experimental-study-of-a-rat-model-of-emphysema-induced-by-cigarette-smoke-exposure-and-the-effect-of-survanta-therapy
#10
Assmaa O Selim, Zienab A Gouda, Sally A Selim
The present study was performed to test the therapeutic effects of Survanta (an exogenous surfactant) on a Wistar rat model of emphysema. Thirty-five adult male Wistar rats were divided randomly into the following groups; control subgroups Ia&b (n=14); emphysematous model subgroups IIa,b&c (n=21) exposed to cigarette smoke (CS), received phosphate buffer solution (PBS) and Survanta respectively. The levels of serum myeloperoxidase (MPO), lung tissue lactate dehydrogenase (LDH), alkaline phosphatase (ALP) as well as antioxidants: catalase (CAT), superoxide dismutase (SOD) and oxidative stress: malondialdehyde (MDA) markers were measured...
February 13, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28197209/effect-of-bmp-2-delivery-mode-on-osteogenic-differentiation-of-stem-cells
#11
Taekhee Jung, John Hwan Lee, Soonjung Park, Yong-Jin Kim, Joseph Seo, Hye-Eun Shim, Ki-Suk Kim, Hyon-Seok Jang, Hyung-Min Chung, Seong-Geun Oh, Sung-Hwan Moon, Sun-Woong Kang
Differentiation of stem cells is an important strategy for regeneration of defective tissue in stem cell therapy. Bone morphogenetic protein-2 (BMP-2) is a well-known osteogenic differentiation factor that stimulates stem cell signaling pathways by activating transmembrane type I and type II receptors. However, BMPs have a very short half-life and may rapidly lose their bioactivity. Thus, a BMP delivery system is required to take advantage of an osteoinductive effect for osteogenic differentiation. Previously, BMP delivery has been designed and evaluated for osteogenic differentiation, focusing on carriers and sustained release system for delivery of BMPs...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28192355/enhancement-of-struvite-pellets-crystallization-in-a-full-scale-plant-using-an-industrial-grade-magnesium-product
#12
D Crutchik, N Morales, J R Vázquez-Padín, J M Garrido
A full-scale struvite crystallization system was operated for the treatment of the centrate obtained from the sludge anaerobic digester in a municipal wastewater treatment plant. Additionally, the feasibility of an industrial grade Mg(OH)2 as a cheap magnesium and alkali source was also investigated. The struvite crystallization plant was operated for two different periods: period I, in which an influent with low phosphate concentration (34.0 mg P·L(-1)) was fed to the crystallization plant; and period II, in which an influent with higher phosphate concentration (68...
February 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28187879/validated-stability-indicating-hplc-dad-method-for-determination-of-the-recently-approved-hepatitis-c-antiviral-agent-daclatasvir
#13
M M Baker, D S El-Kafrawy, M S Mahrous, T S Belal
A comprehensive stability indicating HPLC with diode array detection method was developed for the determination of the recently approved antiviral drug daclatasvir dihydrochloride (DCV) which is used for the treatment of chronic Hepatitis C Virus (HCV) genotype 3 infection. Effective chromatographic separation was achieved using Waters C8 column (4.6×250mm, 5μm particle size) with isocratic elution of the mobile phase composed of mixed phosphate buffer pH 2.5 and acetonitrile in the ratio of 75:25 (by volume)...
February 7, 2017: Annales Pharmaceutiques Françaises
https://www.readbyqxmd.com/read/28186955/cross-sectional-analysis-of-universal-vitamin-d-supplementation-in-former-east-germany-during-the-first-year-of-life
#14
Aris Siafarikas, Alfons Deichl, Gerhard Jahreis, Angela Pieplow, Hartmut Vogel, Eberhard Kauf, Anna-Elisabeth Kapuhs, Elke Badeke, Günter Berger, Hans Kändler, Volker Hesse
BACKGROUND: Universal vitamin D supplementation is controversial. Preventative examinations and public health initiatives in former East Germany that included vitamin D prophylaxis for children were regulated by official recommendations and guidelines. The aim of this study is to analyse the impact of a standardised nationwide guideline for universal supplementation with 400 International Units (IU) vitamin D3/day during the first year of life on clinical and biochemical parameters and the influence of surrounding factors...
February 10, 2017: Journal of Pediatric Endocrinology & Metabolism: JPEM
https://www.readbyqxmd.com/read/28185176/effect-of-coexisting-components-on-phosphate-adsorption-using-magnetite-particles-in-water
#15
Won-Hee Lee, Jong-Oh Kim
In this study, we focused on the rate of adsorption of phosphate on to the surface of magnetite in the presence of coexisting anions, organic matters and heavy metals. Magnetite particles were prepared using a co-precipitation method. Iron (II) sulfate heptahydrate and iron (III) chloride hexahydrate were mixed and then a sodium hydroxide solution was added drop-wise in the mixed iron solution. Coexisting anions were found to have no effect on the decrease in phosphate adsorption. However, phosphate adsorbed on to magnetite surface decreased with increasing total organic carbon (TOC) concentration of natural organic matter (NOM) such as citric, oxalic, and humic acid...
February 9, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28183648/evaluation-of-an-injectable-bioactive-borate-glass-cement-to-heal-bone-defects-in-a-rabbit-femoral-condyle-model
#16
Xu Cui, Wenhai Huang, Yadong Zhang, Chengcheng Huang, Zunxiong Yu, Lei Wang, Wenlong Liu, Ting Wang, Jie Zhou, Hui Wang, Nai Zhou, Deping Wang, Haobo Pan, Mohamed N Rahaman
There is a need for synthetic biomaterials to heal bone defects using minimal invasive surgery. In the present study, an injectable cement composed of bioactive borate glass particles and a chitosan bonding solution was developed and evaluated for its capacity to heal bone defects in a rabbit femoral condyle model. The injectability and setting time of the cement in vitro decreased but the compressive strength increased (8±2MPa to 31±2MPa) as the ratio of glass particles to chitosan solution increased (from 1...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28181569/long-term-nitrogen-fertilization-decreased-the-abundance-of-inorganic-phosphate-solubilizing-bacteria-in-an-alkaline-soil
#17
Bang-Xiao Zheng, Xiu-Li Hao, Kai Ding, Guo-Wei Zhou, Qing-Lin Chen, Jia-Bao Zhang, Yong-Guan Zhu
Inorganic phosphate solubilizing bacteria (iPSB) are essential to facilitate phosphorus (P) mobilization in alkaline soil, however, the phylogenetic structure of iPSB communities remains poorly characterized. Thus, we use a reference iPSB database to analyze the distribution of iPSB communities based on 16S rRNA gene illumina sequencing. Additionally, a noval pqqC primer was developed to quantify iPSB abundance. In our study, an alkaline soil with 27-year fertilization treatment was selected. The percentage of iPSB was 1...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28178619/effect-of-qishenyiqi-pill-on-myocardial-collagen-metabolism-in-experimental-autoimmune-myocarditis-rats
#18
Shi-Chao Lv, Meifang Wu, Meng Li, Qiang Wang, Xiao-Jing Wang, Ao Zhang, Ling Xu, Jun-Ping Zhang
OBJECTIVE: To observe the effect of QiShenYiQi pill (QSYQ) on myocardial collagen metabolism in experimental autoimmune myocarditis rats, and to explore its mechanism of action. METHODS: Lewis rats underwent the injection of myocardial myosin mixed with freund's complete adjuvant were randomized into three groups: model, valsartan and QSYQ groups. And we treated rats which were injected phosphate buffered saline (PBS) mixed with freund's complete adjuvant as control group...
February 5, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28177802/plant-growth-promotion-properties-of-bacterial-strains-isolated-from-the-rhizosphere-of-the-jerusalem-artichoke-helianthus-tuberosus-l-adapted-to-saline-alkaline-soils-and-their-effect-on-wheat-growth
#19
Xiaolin Liu, Xiangyue Li, Yan Li, Runzhi Li, Zhihong Xie
The Jerusalem artichoke (JA; Helianthus tuberosus), known to be tolerant to saline-alkaline soil conditions, has been cultivated for many years in the Yellow River delta, Shandong Province coastal zone, in China. The aim of our study was to isolate nitrogen-fixing bacteria colonizing the rhizosphere of JA and to characterize other plant growth promotion properties. The ultimate goal was to identify isolates that could be used as inoculants benefiting an economic crop, in particular for improving wheat growth production in the Yellow River delta...
November 14, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28176961/blooming-gelatin-an-individual-additive-for-enhancing-nanoapatite-precipitation-physical-properties-and-osteoblastic-responses-of-nanostructured-macroporous-calcium-phosphate-bone-cements
#20
Ziba Orshesh, Saeed Hesaraki, Ali Khanlarkhani
In recent years, there has been a great interest in using natural polymers in the composition of calcium phosphate bone cements to enhance their physical, mechanical, and biological performance. Gelatin is a partially hydrolyzed form of collagen, a natural component of bone matrix. In this study, the effect of blooming gelatin on the nanohydroxyapatite precipitation, physical and mechanical properties, and cellular responses of a calcium phosphate bone cement (CPC) was investigated. Various concentrations of blooming gelatin (2, 5, and 8 wt...
2017: International Journal of Nanomedicine
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