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https://www.readbyqxmd.com/read/28636187/dual-labeled-chemiluminescence-enzyme-immunoassay-for-simultaneous-measurement-of-total-prostate-specific-antigen-tpsa-and-free-prostate-specific-antigen-fpsa
#1
Lixia Zhao, Dan Wang, Gen Shi, Ling Lin
The specificity for early diagnostic of prostate-specific antigen (PSA) is low because the current technology mostly allows the detection of only one biomarker at one time. In this work, a dual-labeled chemiluminescence enzyme immunoassay (CLEIA) for simultaneous measurement of total PSA (TPSA) and free PSA (FPSA) was proposed. Anti-PSA McAb (Mab1) was immobilized on a microplate as the solid phase, horseradish peroxidase (HRP)-labeled anti-TPSA monoclonal antibody (McAb2) and alkaline phosphatase (ALP)-labeled anti-FPSA McAb3 were used as detection antibodies...
June 21, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28634802/alkaline-treated-sawdust-as-an-effective-material-for-cationic-dye-removal-from-textile-effluents-under-dynamic-conditions-breakthrough-curve-prediction-and-mechanism-exploration
#2
Ahmed Amine Azzaz, Salah Jellali, Rahma Souissi, Karim Ergaieg, Latifa Bousselmi
This paper deals with the methylene blue molecule (MB) removal from synthetic and real textile wastewaters by alkali-treated orange tree sawdust (ATOS) under different dynamic conditions. Experimental results showed that MB removal efficiencies by ATOS increased when increasing initial dye concentrations and bed depths but decreased with the increase of the applied flow rates with a maximum adsorption capacity of about 110 mg g(-1). Moreover, various empirical models were applied to predict the experimental breakthrough curves (BTCs) and to determine the characteristic adsorption parameters...
June 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28631614/3d-printed-bioceramic-scaffolds-with-antibacterial-and-osteogenic-activity
#3
Yongliang Zhang, Dong Zhai, Mengchi Xu, Qingqiang Yao, Huiying Zhu, Jiang Chang, Chengtie Wu
Bacterial infection poses a significant risk with the wide application of bone graft materials. Designing bone grafts with good antibacterial performance and excellent bone-forming activity is of particular significance for bone tissue engineering. In our study, a 3D printing method was used to prepare β-tricalcium phosphate (β-TCP) bioceramic scaffolds. Silver (Ag) nanoparticles were uniformly dispersed on graphene oxide (GO) to form a homogeneous nanocomposite (named Ag@GO) with different Ag-to-graphene oxide mass ratios, with this being synthesized via the liquid chemical reduction approach...
June 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28629105/indispensable-role-of-biochar-inherent-mineral-constituents-in-its-environmental-applications-a-review
#4
Xiaoyun Xu, Yinghao Zhao, Jingke Sima, Ling Zhao, Ondřej Mašek, Xinde Cao
Biochar typically consists of both carbon and mineral fractions, and the carbon fraction has been generally considered to determine its properties and applications. Recently, an increasing body of research has demonstrated that mineral components inherent in biochar, such as alkali or alkaline earth metals in the form of carbonates, phosphates, or oxides, could also influence the properties and thus the applications. The review articles published thus far have mainly focused on multiple environmental and agronomic applications of biochar, including carbon sequestration, soil improvement, environmental remediation, etc...
June 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28628862/characterization-and-inhibition-studies-of-tissue-nonspecific-alkaline-phosphatase-by-aminoalkanol-derivatives-of-1-7-dimethyl-8-9-diphenyl-4-azatricyclo-5-2-1-0-2-6-dec-8-ene-3-5-10-trione-new-competitive-and-non-competitive-inhibitors-by-capillary-electrophoresis
#5
Błażej Grodner, Mariola Napiórkowska
The article describes the inhibitory effect of two new aminoalkanol derivatives on the enzymatic kinetic of tissue non-specific alkaline phosphatase with use of capillary zone electrophoresis to evaluate the inhibitory effect. This technique allows to investigate of the enzymatic kinetic by the measure of the amounts of the substrate and product in the presence of compound (I) or (II) in the reaction mixture. The separation process was conducted using an eCAP fused-silica capillary. The detector was set at 200nm...
June 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28622916/osteogenic-potential-of-platelet-rich-plasma-in-dental-stem-cell-cultures
#6
L Otero, N Carrillo, J L Calvo-Guirado, J Villamil, R A Delgado-Ruíz
The purpose of this study was to analyse the potential of platelet-rich plasma (PRP) culture media to induce osteogenic differentiation of periodontal ligament stem cells and dental pulp stem cells compared with four other methods of culture. Both types of cell were collected from 35 healthy patients and cultured in five different media (Dulbecco's modified eagle's medium (DMEM); DMEM and melatonin; DMEM and PRP; DMEM and ascorbic acid 200μmol; DMEM and l-ascorbate 2-phosphate 50μmol). Cells were characterised by flow cytometry...
June 13, 2017: British Journal of Oral & Maxillofacial Surgery
https://www.readbyqxmd.com/read/28622088/engineering-osteoinductive-biomaterials-by-bioinspired-synthesis-of-apatite-coatings-on-collagen-hydrogels-with-varied-pore-microarchitectures
#7
Jacqueline Lorraine Harding, Matthew Osmond, Melissa Diane Krebs
Biomaterial controlled osteoinduction is influenced by the porous microenvironment and the composition of incorporated calcium orthophosphate (CaPi) polymorphs, however for the design of materials that rival the efficacies of natural grafts a systematic approach to assessing the physicochemical properties that affects cellular differentiation is needed. In this research, we introduce a bioinspired synthetic approach to the mineralization of preformed porous collagen hydrogel scaffolds with tunable apatite coatings...
June 16, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28617270/flotation-separation-of-strontium-via-phosphate-precipitation
#8
Luong H V Thanh, J C Liu
Flotation separation of strontium (Sr) from wastewater via phosphate precipitation was investigated. While 37.33% of Sr precipitated at highly alkaline pH in the absence of PO4(3-), it completely precipitated as Sr3(PO4)2 at a molar ratio ([PO4(3-)]:[Sr(2+)]) of 0.62 at a lower pH value. The presence of Ca(2+) hindered Sr precipitation, yet it could be overcome by increasing the PO4(3-) dose. Sodium dodecyl sulfate (SDS) was a better collector for dispersed air flotation of Sr3(PO4)2 than cetyl trimethyl ammonium bromide, or mixed collector systems of SDS and saponin...
June 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28612542/-establishment-of-bone-metastasis-model-of-prostate-cancer-in-mice-with-monitoring-of-18-f-naf-pet-ct
#9
Qiang You, Hua-Wei Cai, Fu-Wen Pang, Zhe-Tao Wang, Yue Chen, Rong Tian, Lin Li, Li-Hong Chen
OBJECTIVES: To establish a mouse model bearing human prostate cancer xenograft with bone metastasis by the monitoring with X-Ray, Micro CT, and ¹⁸F-NaF PET/CT. METHODS: Sixteen male Balb/c nude mice were allocated into control (6 mice) and experimental group (10 mice). In experimental group, the mice were subjected to percutaneous injection of 2×10(5)PC-3 cells into tibial plateau, bone defects were assessed after 21 d by X-ray, Micro-CT and ¹⁸F-NaF PET/CT, and bone damages were evaluated by HE staining...
March 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28606051/comparison-of-bone-mineral-density-in-common-variable-immunodeficiency-and-x-linked-agammaglobulinaemia-patients
#10
Ali Mohebbi, Gholamreza Azizi, Naeimeh Tavakolinia, Mehdi Karimipour, Fatemeh Kiaee, Reza Yazdani, Sareh Sadat Ebrahimi, Hosein Rafiemanesh, Vahid Ziaee, Hassan Abolhassani, Asghar Aghamohammadi, Farzaneh Abbasi, Fatemeh Sayarifard, Mehran Ebrahimi, Javad Tafaroji
BACKGROUND: Primary antibody deficiency (PAD) is the most common group of primary immunodeficiency disorders, resulting from different defects in development and function of B cell lineage. Common variable immunodeficiency (CVID) and X-linked agammaglobulinemia (XLA) are two of the major types of PADs. Optimal growth and subsequently bone health could potentially compromise due to the interference of several factors in PAD with childhood onset. In the present study, our aim was to evaluate bone mineral density (BMD) of patients with CVID and XLA...
June 11, 2017: Endocrine, Metabolic & Immune Disorders Drug Targets
https://www.readbyqxmd.com/read/28605646/imidacloprid-application-changes-microbial-dynamics-and-enzymes-in-rice-soil
#11
Bibhab Mahapatra, Totan Adak, Naveen K B Patil, Guru P Pandi G, G Basana Gowda, N N Jambhulkar, Manoj Kumar Yadav, P Panneerselvam, Upendra Kumar, Sushmita Munda, Mayabini Jena
Extensive use of imidacloprid in rice ecosystem may alter dynamics of microorganisms and can change soil biochemical properties. The objective of this study was to assess the effect of imidacloprid on growth and activities of microbes in tropical rice soil ecosystem. Four treatments, namely, recommended dose (at 25g a.i. ha(-1), RD), double the recommended dose (at 50g a.i. ha(-1), 2RD), five times the recommended dose (at 125g a.i. ha(-1), 5RD) & ten times the recommended dose (at 250g a.i. ha(-1), 10RD) along with control were imposed under controlled condition...
June 9, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28604622/the-understanding-of-the-metazoan-skeletal-system-based-on-the-initial-discoveries-with-siliceous-and-calcareous-sponges
#12
REVIEW
Werner E G Müller, Heinz C Schröder, Xiaohong Wang
Initiated by studies on the mechanism of formation of the skeletons of the evolutionary oldest still extant multicellular animals, the sponges (phylum Porifera) have provided new insights into the mechanism of formation of the Ca-phosphate/hydroxyapatite skeleton of vertebrate bone. Studies on the formation of the biomineral skeleton of sponges revealed that both the formation of the inorganic siliceous skeletons (sponges of the class of Hexactinellida and Demospongiae) and of the calcareous skeletons (class of Calcarea) is mediated by enzymes (silicatein: polymerization of biosilica; and carbonic anhydrase: deposition of Ca-carbonate)...
June 12, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28603126/prophylactic-and-therapeutic-effect-of-punica-granatum-in-trinitrobenzene-sulfonic-acid-induced-inflammation-in-rats
#13
Azra Riaz, Rafeeq Alam Khan, Syeda Afroz, Neelam Mallick
Pomegranate (Punica granatum L., Punicaceae) contains varieties of antioxidants and phytochemicals; there are evidences that phytochemicals and antioxidants play a vital role in reducing inflammation. Hence this investigation was planned to assess the outcome of Punica granatum on trinitrobenzene sulfonic acid provoked colitis in rats at 2, 5 and 8ml/kg of the body weight. The effect of P. granatum was assessed in two group i.e. prophylaxis as pre-colitis and therapeutic as post-colitis. After completion of dosing in both the groups, macroscopic and histological examination of colon was carried out along with estimation of serum myeloperoxidase, glutathione, alkaline phosphate, fibrinogen and C-reactive protein...
January 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28595806/conversion-of-biowastes-to-biomaterial-an-innovative-waste-management-approach
#14
M Bobby Kannan, Karly Ronan
The study suggests that biowastes (eggshells and urine) can be potentially used as precursors to produce hydroxyapatite (HAp) biomaterial in a simple chemical process. A batch reactor was used in this work to produce HAp powder from eggshells and synthetic urine (SU). Fine powder of calcined eggshells was dissolved in water to produce aqueous calcium hydroxide. The solution was then mixed with concentrated SU in stoichiometric amounts corresponding to HAp (Ca/P molar ratio∼1.67). The initial pH of the solution was alkaline (pH∼8...
June 5, 2017: Waste Management
https://www.readbyqxmd.com/read/28583636/metabolic-profile-of-liver-damage-in-non-cirrhotic-virus-c-and-autoimmune-hepatitis-a-proton-decoupled-31-p-mrs-study
#15
Antti Hakkarainen, Lauri Puustinen, Reetta Kivisaari, Sonja Boyd, Urpo Nieminen, Perttu Arkkila, Nina Lundbom
PURPOSE: To study liver (31)P MRS, histology, transient elastography, and liver function tests in patients with virus C hepatitis (HCV) or autoimmune hepatitis (AIH) to test the hypothesis that (31)P MR metabolic profile of these diseases differ. MATERIALS AND METHODS: 25 patients with HCV (n=12) or AIH (n=13) underwent proton decoupled (31)P MRS spectroscopy performed on a 3.0T MR imager. Intensities of phosphomonoesters (PME) of phosphoethanolamine (PE) and phosphocholine (PC), phosphodiesters (PDE) of glycerophosphoethanolamine (GPE) and glycerophosphocholine (GPC), and γ, α and β resonances of adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate (NADPH) were determined...
May 2017: European Journal of Radiology
https://www.readbyqxmd.com/read/28580479/highly-sensitive-novel-cathodic-electrochemiluminescence-of-tris-2-2-bipyridine-ruthenium-ii-using-glutathione-as-a-co-reactant
#16
Chikkili Venkateswara Raju, Shanmugam Senthil Kumar
Herein, glutathione was used as a co-reactant for the first time to generate a novel, highly stable, and enhanced cathodic electrochemiluminescence (ECL) on a simple bare glassy carbon electrode (GCE) surface using the Ru(bpy)3(2+) molecule in an alkaline pH phosphate buffer solution (PBS).
June 16, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28578273/biomimetic-mineralized-hierarchical-hybrid-scaffolds-based-on-in-situ-synthesis-of-nano-hydroxyapatite-chitosan-chondroitin-sulfate-hyaluronic-acid-for-bone-tissue-engineering
#17
Yimin Hu, Jingdi Chen, Tiantang Fan, Yujue Zhang, Yao Zhao, Xuetao Shi, Qiqing Zhang
Biomimetic mineralized hybrid scaffolds are widely used as natural bone substitute materials in tissue engineering by mimicking vital characters of extracellular matrix (ECM). However, the fabrication of hybrid scaffolds with suitable mechanical properties and good biocompatibility remains a challenge. To solve the problems mentioned above, biomimetic calcium phosphate mineralized organic-inorganic hybrid scaffold composed of nano hydroxyapatite (nHAP), Chitosan (CS), Chondroitin sulfate (CSA) and hyaluronic acid (HA) with hierarchical micro/nano structures was successfully developed...
May 25, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28575927/storage-induced-changes-in-coliform-heterotrophic-groups-of-bacteria-and-nutrient-levels-of-human-urine-for-its-safe-use-in-biological-production
#18
B B Jana, Susmita Lahiri, D Sarkar, Sukanta Rana, J N Bhakta, Dipak Paul
Human urine is a potential source of various nutrients, minerals and trace elements. Its use as a fertilizer is growing popular among farmers. Here, we examined the pattern of changes in the counts of coliform, heterotrophic bacteria as well as physico-chemical characteristics of human urine during different days of storage under closed conditions at ambient temperature. We observed that after 253 days of storage under closed condition, the coliform counts were reduced significantly and remained within the safe limit to be used as fertilizer...
August 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/28570775/fe2-po5-encapsulated-reverse-energetic-zno-fe2-o3-heterojunction-nanowire-for-enhanced-photoelectrochemical-oxidation-of-water
#19
Dong-Dong Qin, Cai-Hua He, Yang Li, Antonio C Trammel, Jing Gu, Jing Chen, Yong Yan, Duo-Liang Shan, Qiu-Hong Wang, Jing-Jing Quan, Chun-Lan Tao, Xiao-Quan Lu
Zinc oxide is regarded as a promising candidate for application in photoelectrochemical water oxidation due to its higher electron mobility. However, its instability under alkaline conditions limits its application in a practical setting. Herein, we demonstrate an easily achieved wet-chemical route to chemically stabilize ZnO nanowires (NWs) by protecting them with a thin layer Fe2 O3 shell. This shell, in which the thickness can be tuned by varying reaction times, forms an intact interface with ZnO NWs, thus protecting ZnO from corrosion in a basic solution...
June 1, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28558021/interactions-of-sclerostin-with-fgf23-soluble-klotho-and-vitamin-d-in-renal-transplantation
#20
Lida Tartaglione, Marzia Pasquali, Silverio Rotondi, Maria Luisa Muci, Cristiana Leonangeli, Alessio Farcomeni, Valeria Fassino, Sandro Mazzaferro
Relationships of Sclerostin, a bone anti-anabolic protein, with biomarkers of mineral bone disorders in chronic kidney disease are still unsettled, in particular in kidney transplant (KTR). In 80 KTR patients (31F/49M, 54.7±10.3 years) we studied the relationships of serum Sclerostin with eGFR, Calcium, Phosphate, Alkaline Phosphatase (AP), intact Parathyroid hormone (iPTH), soluble alpha-Klotho (sKlotho), intact Fibroblast Growth Factor 23 (iFGF23), 25-hydroxyvitamin D(25D) and 1,25-dihydroxyvitamin D (1,25D)...
2017: PloS One
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