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https://www.readbyqxmd.com/read/28742069/nematode-grazing-promotes-bacterial-community-dynamics-in-soil-at-the-aggregate-level
#1
Yuji Jiang, Manqiang Liu, Jiabao Zhang, Yan Chen, Xiaoyun Chen, Lijun Chen, Huixin Li, Xue-Xian Zhang, Bo Sun
Nematode predation has important roles in determining bacterial community composition and dynamics, but the extent of the effects remains largely rudimentary, particularly in natural environment settings. Here, we investigated the complex microbial-microfaunal interactions in the rhizosphere of maize grown in red soils, which were derived from four long-term fertilization regimes. Root-free rhizosphere soil samples were separated into three aggregate fractions whereby the abundance and community composition were examined for nematode and total bacterial communities...
July 25, 2017: ISME Journal
https://www.readbyqxmd.com/read/28741110/biosensor-of-alkaline-phosphatase-based-on-non-fluorescent-fret-of-eu-3-doped-oxide-nanoparticles-and-phosphorylated-peptide-labeled-with-cyanine-dye
#2
Fan Shi Li, Ya Ling Zhang, Xia Bing Li, Bao Lin Li, Yi Feng Liu
Dephosphorylation of biomolecules under the catalysis of alkaline phosphatase (ALP) is a critical physiological process. Abnormal levels of ALP activity have been associated with a number of diseases; thus, a simple and sensitive assay of ALP activity is highly demanded. Herein, to simulate biological conditions, we labeled a hydrosoluble phosphorylated heptapeptide Gly-Pro-Gly-Asn-p-Tyr-Gly-Ala (pGA) with aminated heptamethine cyanine dye (Cy) to give a low fluorescent labeled peptide Cy-pGA. The synthesized Cy-pGA and Eu(3+)-doped oxide Y0...
July 24, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28740939/effect-of-a-short-period-whole-body-vibration-with-10-hz-on-blood-biomarkers-in-wistar-rats
#3
Milena de Oliveira Bravo Monteiro, Danúbia da Cunha de Sá-Caputo, Eloá Moreira-Marconi, Éric Heleno Freire Ferreira Frederico, Cintia Renata de Sousa-Gonçalves, Luciana Camargo Bernardo, Carlos Alberto Sampaio Guimarães, Mario Bernardo-Filho
BACKGROUND: Exposure to whole body vibration exercises (WBVE), besides some biological effects, causes alterations in the concentration of some blood biomarkers. The aim of this study is to evaluate the action of vibration (10 Hz) of WBVE on the concentration of blood biomarkers in Wistar rats. MATERIALS AND METHODS: Wistar rats were divided in 2 groups. The experimental group (EG) was subjected to vibrations of 10Hz (one min per day, one week, total time of seven min), while the control group (CG) has not experienced vibration...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28737140/bone-mineral-density-of-indian-children-and-adolescents-with-cystic-fibrosis
#4
Sumita Gupta, Aparna Mukherjee, Rajesh Khadgawat, Madhulika Kabra, Rakesh Lodha, Sushil K Kabra
OBJECTIVE: To document bone mineral density of children and adolescents with cystic fibrosis. DESIGN: Cross-sectional study. SETTING: Tertiary-care center of Northern India, July 2012 to August 2015. PARTICIPANTS: 52 children aged 6-18 years with cystic fibrosis and 62 healthy controls of similar age and sex. METHODS: Both patients and controls were stratified into two groups, as pre-pubertal and peri-/post-pubertal, and compared for whole body bone mineral density, measured using dual energy X-ray absorptiometry...
July 15, 2017: Indian Pediatrics
https://www.readbyqxmd.com/read/28735787/chemical-physical-properties-and-apatite-forming-ability-of-mineral-trioxide-aggregate-flow
#5
Bruno Martini Guimarães, Rodrigo Ricci Vivan, Bruno Piazza, Murilo Priori Alcalde, Clovis Monteiro Bramante, Marco Antonio Hungaro Duarte
INTRODUCTION: This study aimed to analyze the chemical-physical properties, including pH, volumetric change, radiopacity, and apatite-forming ability in simulated body fluid, of a new tricalcium silicate material (MTA Flow; Ultradent Products Inc, South Jordan, UT). METHODS: MTA Flow was tested in comparison with MTA Angelus (Angelus, Londrina, PR, Brazil). The pH of soaking water was tested up to 168 hours in deionized water. In the solubility test, the root-end fillings of 20 acrylic teeth were scanned twice by micro-computed tomographic imaging before and after immersion in ultrapure water for 168 hours...
July 20, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28735065/time-required-for-resetting-postural-effects-on-serum-constituents-in-healthy-individuals
#6
Yoshihisa Shimizu, Kiyoshi Ichihara, Katsunori Kouguchi
OBJECTIVE: We investigated postural effects on concentrations of commonly measured serum analytes and sought for optimal duration of sitting before venipuncture in an outpatient setting. METHODS: Seven healthy volunteers changed posture serially at 30-minute (min) intervals from lying, sitting, slowly walking, sitting, and to lying again. At the end of the first lying posture, wing-needle was inserted into cubital vein and 3mL of blood was drawn every 5min. All the serum was stored at -80°C until the time of collective measurements for 16 major chemistry analytes two weeks later...
July 19, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28718291/porous-structured-ni-fe-p-nanocubes-derived-from-prussian-blue-analogue-as-electrocatalyst-for-efficient-overall-water-splitting
#7
Cuijuan Xuan, Jie Wang, Weiwei Xia, Zongkai Peng, Zexing Wu, Wen Lei, Kedong Xia, Huolin L Xin, Deli Wang
Exploring non-precious metal electrocatalysts to replace the noble metal based catalysts for full water electrocatalysis is still an ongoing challenge. In this work, porous structured ternary nickel-iron-phosphides (Ni-Fe-P) nanocubes were synthesized through one-step phosphidation of Ni-Fe based Prussian blue analogue. The Ni-Fe-P nanocubes exhibit rough and loose porous structure on the surface under suitable phosphating temperature, which is favorable for the mass transfer and oxygen diffusion during electrocatalysis process...
July 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28714662/enhancing-the-osteogenic-capability-of-core-shell-bilayered-bioceramic-microspheres-with-adjustable-biodegradation
#8
Xiurong Ke, Chen Zhuang, Xianyan Yang, Jia Fu, Sanzhong Xu, Lijun Xie, Zhongru Gou, Juncheng Wang, Lei Zhang, Guojing Yang
This study describes the fabrication and biological evaluation of core-shell bilayered bioceramic microspheres with adjustable compositional distribution via a coaxial bilayer capillary system. Beyond the homogeneous hybrid composites, varying the diameter of capillary nozzles and the composition of the bioceramic slurries makes it easy to create bilayered β-tricalcium phosphate (CaP)/β-calcium silicate (CaSi) microspheres with controllable compositional distribution in the core or shell layer. Primary investigations in vitro revealed that biodegradation could be adjusted by compositional distribution or shell thickness and that poorly soluble CaP located on the shell layer of CaP or CaSi@CaP microspheres was particularly beneficial for mesenchymal stem cell adhesion and growth in the early stage, but the ion release from the CaP@CaSi exhibited a potent stimulating effect on alkaline phosphatase expression of the cells at longer times...
July 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28714271/influence-of-residual-renal-function-in-carotid-modeling-as-a-marker-of-early-atherosclerosis-in-dialysis-patients
#9
Merita Rroji, Nereida Spahia, Saimir Seferi, Myftar Barbullushi, Goce Spasovski
Atherosclerosis is frequently present in patients with chronic kidney disease (CKD) treated with dialysis. We evaluated the association between residual renal function (RRF), phosphate level, inflammation and other risk factors in carotid modeling as a marker of early atherosclerosis in peritoneal dialysis (PD) compared with hemodialysis (HD) patients. We studied 39 stable PD and 53 HD patients on renal replacement therapy (RRT) for 3 to 36 months duration. B-mode ultrasonography was used to determine carotid artery intima media thickness (CIMT)...
July 17, 2017: Therapeutic Apheresis and Dialysis
https://www.readbyqxmd.com/read/28713731/magnetic-field-effects-on-bone-repair-after-calcium-phosphate-cement-implants-histometric-and-biochemistry-evaluation
#10
Thiago Calcagnotto, Marcos Miguel Bechstedt Schwengber, Carlos Cesar De Antoni, Danilo Louzada de Oliveira, Théssio Mina Vago, Jessica Guilinelli
OBJECTIVE: This work evaluated histologic and biochemically the effect of magnetic field buried in bone repair after autogenous bone graft and calcium phosphate cement implants. METHODOLOGY: Bone defects with 5,0 mm of diameter in the cranium of Wistar mice were used to analyse. These mice were submitted to different interventions: autogenous bone graft and calcium phosphate cement implants, both with and without magnetic stimulation. Longitudinal and transversal histometric and biochemistry analysis were made in times of 15, 30 and 60 post-operative days...
January 2017: Annals of Maxillofacial Surgery
https://www.readbyqxmd.com/read/28708908/activation-of-osteoblastic-function-on-titanium-surface-with-titanium-doped-hydroxyapatite-nanoparticle-coating-an-in-vitro-study
#11
Masahiro Nakazawa, Masahiro Yamada, Masato Wakamura, Hiroshi Egusa, Kaoru Sakurai
PURPOSE: Titanium-doped hydroxyapatite (TiHA) nanoparticles contain titanium atoms in the hydroxyapatite lattice, which can physicochemically functionalize the titanium surface without modification of the surface topography. This study aimed to evaluate the physicochemical properties of machined or microroughened titanium surfaces coated with TiHA nanoparticles and the functions of osteoblasts cultured on them. MATERIALS AND METHODS: Titanium disks with commercially available surface topography, such as machined or sandblasted, large-grit, and acid-etched (SLA) surfaces, were coated with TiHA...
July 2017: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/28696095/dt-diaphorase-as-a-bifunctional-enzyme-label-that-allows-rapid-enzymatic-amplification-and-electrochemical-redox-cycling
#12
Cheolho Kang, Juyeon Kang, Nam-Sihk Lee, Young Ho Yoon, Haesik Yang
The most common enzyme labels in enzyme-linked immunosorbent assays are alkaline phosphatase and horseradish peroxidase, which, however, have some limitations for use in electrochemical immunosensors. This Article reports that the small and thermostable DT-diaphorase (DT-D) and electrochemically inactive 4-nitroso-1-naphthol (4-NO-1-N) can be used as a bifunctional enzyme label and a rapidly reacting substrate, respectively, for electrochemical immunosensors. This enzyme-substrate combination allows high signal amplification via rapid enzymatic amplification and electrochemical redox cycling...
July 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28691695/mechanical-and-biological-characterization-of-alkaline-substituted-orthophosphate-bone-substitutes-containing-meta-and-diphosphates
#13
Moritz Klein, Matthias W Laschke, Joerg H Holstein, Tina Histing, Tim Pohlemann, Michael Menger, Patric Garcia
Despite the growing knowledge on the mechanisms of fracture healing, bone defects often do not heal in a timely manner. Clinically, tricalcium phosphate (Ca-P) bone substitutes are used to fill bone defects and promote bone healing. However, the degradation rate of these implants is often too slow for sufficient bone replacement. The use of calcium phosphate material with the crystalline phase Ca10 [K/Na](PO4)7 containing different amounts of di- and metaphosphates may overcome this problem, because these materials show an accelerated degradation...
July 10, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28689456/chest-deformity-and-disability-due-to-tenofovir-induced-hypophosphatemic-osteomalacia-case-report-and-call-for-improved-global-access-to-laboratory-testing
#14
Jan Hajek, Simple Ouma, Juliya Hemmett, Rob Starko, Paska Apiyo
Millions of people worldwide take tenofovir disoproxil fumarate (TDF) for the treatment of human immunodeficiency virus (HIV) and/or hepatitis B infection. Although generally safe and well tolerated, clinicians need to be aware that TDF can cause proximal renal tubular dysfunction and loss of bone mineral density, especially in patients with concomitant renal disease or other risk factors. We present the case of a patient with chronic HIV infection and urethral stricture who developed TDF-related proximal renal tubular dysfunction with hypophosphatemia and osteomalacia, presenting with bone pains, skeletal deformity, and disability...
January 1, 2017: Journal of the International Association of Providers of AIDS Care
https://www.readbyqxmd.com/read/28687877/a-simple-and-sensitive-surface-enhanced-raman-spectroscopic-discriminative-detection-of-organophosphorous-nerve-agents
#15
Jianfeng Wu, Yingjie Zhu, Jing Gao, Jia Chen, Jianlin Feng, Lei Guo, Jianwei Xie
Organophosphorous nerve agents (NAs) pose a great threat to nations and people because of their acute and extreme toxicities. The rapid detection of NAs has attracted growing interest in the first emergency response field. In this work, we demonstrate a simple and sensitive surface-enhanced Raman spectroscopic (SERS) method for NAs detection, and G- and V-agents discrimination. The results show that VX (V-agents) can be directly detected at a 20 ng mL(-1) level with pinhole shell-isolated gold nanoparticles (pinSHINs) as the substrate...
July 7, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28687737/the-chloride-phosphate-ratio-combined-with-alkaline-phosphatase-as-a-valuable-predictive-marker-for-primary-hyperparathyroidism-in-chinese-individuals
#16
Qianqian Wang, Xu Li, Haibing Chen, Haoyong Yu, Lianxi Li, Jun Yin, Jian Zhou, Ming Li, Qing Li, Junfeng Han, Li Wei, Fang Liu, Yuqian Bao, Weiping Jia
The chloride/phosphate ratio (Cl/PO4) has been suggested to have a role in primary hyperparathyroidism (PHPT), but the associations between Cl/PO4 combined with ALP level and PHPT has not been well-studied. Our aim was to investigate the predictive value of combination Cl/PO4 with ALP for PHPT. A cross-sectional retrospective analysis was conducted to examine 172 patients diagnosed with PHPT categorized into two groups: normocalcaemic primary hyperparathyroidism (NPHPT) group and hypercalcaemia PHPT group. We found that Cl/PO4 levels and ALP levels in the NPHPT and hypercalcaemia PHPT group were both significantly higher than normal controls...
July 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28687622/polyphosphate-as-donor-of-high-energy-phosphate-for-the-synthesis-of-adp-and-atp
#17
Werner E G Müller, Shunfeng Wang, Meik Neufurth, Maria Kokkinopoulou, Qingling Feng, Heinz C Schröder, Xiaohong Wang
We studied the potential role of inorganic polyphosphate (polyP) as an energy source for ADP and ATP formation in the extracellular space. Using SaOS-2 we show that matrix vesicles are released into the extracellular space after incubation with polyP. These vesicles contain both the alkaline phosphatase (ALP) and the adenylate kinase (AK). Both enzymes translocate to the cell membrane in response to polyP. To distinguish the process(es) (i) AMP and ADP formation during ALP hydrolysis, from the (ii) ATP generation via the AK reaction, inhibition studies with the AK inhibitor A(5')P5(5')A were performed...
July 7, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28685537/effect-of-xianling-gubao-capsules-on-bone-mineral-density-in-osteoporosis-patients
#18
Z H Wu, X Zhu, C K Xu, Y J Chen, L Zhang, C L Zhang
This paper aimed to analyze the clinical effects of Xianling Gubao capsules on the bone mineral density (BMD) of 94 patients with osteoporosis. After reviewing and analyzing the clinical information in our hospital from January 2015 to January 2016, the patients were divided into a control group and a treatment group with 47 cases in each group. Patients in the control group were treated with routine Western medicine, while the treatment group received Xianling Gubao capsules. Both groups were treated continuously for 3 courses (30 days each course) and had their BMD analyzed and compared...
April 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28684335/structural-and-physico-chemical-analysis-of-calcium-strontium-substituted-near-invert-phosphate-based-glasses-for-biomedical-applications
#19
U Patel, R M Moss, K M Z Hossain, A R Kennedy, E R Barney, I Ahmed, A C Hannon
Neutron diffraction, (23)Na and (31)P NMR and FTIR spectroscopy have been used to investigate the structural effects of substituting CaO with SrO in a 40P2O5·(16-x)CaO·20Na2O·24MgO·xSrO glass, where x is 0, 4, 8, 12 and 16 mol%. The (31)P solid-state NMR results showed similar amounts of Q(1) and Q(2) units for all of the multicomponent glasses investigated, showing that the substitution of Sr for Ca has no effect on the phosphate network. The M-O coordinations (M= Mg, Ca, Sr, Na) were determined for binary alkali and alkaline earth metaphosphates using neutron diffraction and broad asymmetric distributions of bond length were observed, with coordination numbers that were smaller and bond lengths that were shorter than in corresponding crystals...
July 3, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28684278/zeta-potential-changing-self-emulsifying-drug-delivery-systems-containing-phosphorylated-polysaccharides
#20
Janine Griesser, Stephanie Burtscher, Saskia Köllner, Isabelle Nardin, Felix Prüfert, Andreas Bernkop-Schnürch
AIM: The aim of the study was to develop novel zeta potential changing self-emulsifying drug delivery systems (SEDDS) containing phosphorylated polysaccharides. METHODS: Starch and hydroxypropyl starch (HPS) were phosphorylated by utilizing phosphorus pentoxide. The modified starches, starch phosphate (SP) and hydroxypropyl starch phosphate (HPSP), were loaded into SEDDS and investigated regarding particle size, zeta potential, stability and cell viability. The release of immobilized phosphate by intestinal alkaline phosphatase (IAP) was analyzed via malachite green assay...
July 3, 2017: European Journal of Pharmaceutics and Biopharmaceutics
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