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alkaline phosphate

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https://www.readbyqxmd.com/read/28516994/3d-ordered-porous-moxc-x-1-or-2-for-advanced-hydrogen-evolution-and-li-storage
#1
Hong Yu, Haosen Fan, Jiong Wang, Yun Zheng, Zhengfei Dai, Yizhong Lu, Junhua Kong, Xin Wang, Young Jin Kim, Qingyu Yan, Jong-Min Lee
3D ordered porous structures of MoxC are prepared with different Mo to C ratios and tested for two possible promising applications: hydrogen evolution reaction (HER) through water splitting and lithium ion batteries (LIBs). Mo2C and MoC with 3D periodic ordered structures are prepared with a similar process but different precursors. The 3D ordered porous MoC exhibits excellent cycling stability and rate performance as an anode material for LIBs. A discharge capacity of 450.9 mA h g(-1) is maintained up to 3000 cycles at 10...
May 18, 2017: Nanoscale
https://www.readbyqxmd.com/read/28516761/a-facile-and-sensitive-near-infrared-fluorescence-probe-for-the-detection-of-endogenous-alkaline-phosphatase-activity-in-vivo
#2
Song-Jiao Li, Chunyan Li, Yongfei Li, Junjie Fei, Ping Wu, Bin Yang, Juan Ou-Yang, Shi-Xin Nie
Alkaline phosphatase (ALP) is an essential enzyme and widely distributes in a variety of tissues. To date, various nanomaterial and small-molecule fluorescent probes for ALP have been constructed successfully, but the emission wavelengths of these probes are in the ultraviolet or visible range, which is not beneficial for bioimaging. Herein, a merocyanine-based near-infrared (NIR) fluorescent probe named CyP is first synthesized and used to detect ALP activity. The characteristics of probe CyP are as follows: (1) The probe possesses a facile structure, which can be obtained by easy synthetic steps...
May 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28511112/real-time-fluorescence-assay-of-alkaline-phosphatase-in-living-cells-using-boron-doped-graphene-quantum-dots-as-fluorophores
#3
Li Chen, Guancao Yang, Ping Wu, Chenxin Cai
This work reports a convenient and real-time assay of alkaline phosphatase (ALP) in living cells based on a fluorescence quench-recovery process at a physiological pH using the boron-doped graphene quantum dots (BGQDs) as fluorophore. The fluorescence of BGQDs is found to be effectively quenched by Ce(3+) ions because of the coordination of Ce(3+) ions with the carboxyl group of BGQDs. Upon addition of adenosine triphosphate (ATP) into the system, the quenched fluorescence can be recovered by the ALP-positive expressed cells (such as MCF-7 cells) due to the removal of Ce(3+) ions from BGQDs surface by phosphate ions, which are generated from ATP under catalytic hydrolysis of ALP that expressed in cells...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28510419/sunlight-promotes-fast-release-of-hazardous-cadmium-from-widely-used-commercial-cadmium-pigment
#4
Huiting Liu, Han Gao, Mingce Long, Heyun Fu, Pedro J J Alvarez, Qilin Li, Shourong Zheng, Xiaolei Qu, Dongqiang Zhu
Cadmium pigments are widely used in the polymer and ceramic industry. Their potential environmental risk is under debate, being the major barrier for appropriate regulation. We show that 83.0 ± 0.2 % of hazardous cadmium ion (Cd2+) was released from the commercial cadmium sulfoselenide pigment (i.e., cadmium red) in aqueous suspension within 24 h under simulated sunlit conditions. This photo-dissolution process also generated sub-20 nm pigment nanoparticles. Cd2+ release is attributed to the reactions between photogenerated holes and the pigment lattices...
May 16, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28503694/detection-of-early-osteogenic-commitment-in-primary-cells-using-raman-spectroscopy
#5
Stephanie J Smith, Roger Emery, Andrew Pitsillides, Claire E Clarkin, Sumeet Mahajan
Major challenges in the development of novel implant surfaces for artificial joints include osteoblast heterogeneity and the lack of a simple and sensitive in vitro assay to measure early osteogenic responses. Raman spectroscopy is a label-free, non-invasive and non-destructive vibrational fingerprinting optical technique that is increasingly being applied to detect biochemical changes in cells. In this study Raman spectroscopy has been used to obtain bone cell-specific spectral signatures and to identify any changes therein during osteoblast commitment and differentiation of primary cells in culture...
May 15, 2017: Analyst
https://www.readbyqxmd.com/read/28502026/dentin-remineralizing-ability-and-enhanced-antibacterial-activity-of-strontium-and-hydroxyl-ion-co-releasing-radiopaque-hydroxyapatite-cement
#6
R Jayasree, T S Sampath Kumar, S Mahalaxmi, Sireesha Abburi, Y Rubaiya, Mukesh Doble
Dental caries is an infection of the mineralized tooth structures that advances when acid secreted by bacterial action on dietary carbohydrates diffuses and dissolves the tooth mineral leading to demineralization. During treatment, clinicians often remove only the superficial infected tooth structures and retain a part of affected carious dentin to prevent excessive dentin loss and pulp exposure. Calcium hydroxide is used to treat the affected dentin because it is alkaline, induces pulp-dentin remineralization and decreases bacterial infection...
June 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28498908/development-validation-and-application-of-a-stability-indicating-hplc-method-to-quantify-lidocaine-from-polyethylene-co-vinyl-acetate-eva-matrices-and-biological-fluids
#7
Prabhat Bhusal, Manisha Sharma, Jeff Harrison, Georgina Procter, Gavin Andrews, David S Jones, Andrew G Hill, Darren Svirskis
An efficient and cost-effective quantification procedure for lidocaine by HPLC has been developed to estimate lidocaine from an EVA matrix, plasma, peritoneal fluid and intra-articular fluid (IAF). This method guarantees the resolution of lidocaine from the degradation products obtained from alkaline and oxidative stress. Chromatographic separation of lidocaine was achieved with a retention time of 7 min using a C18 column with a mobile phase comprising acetonitrile and potassium dihydrogen phosphate buffer (pH 5...
May 11, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28497318/controlled-ion-release-from-novel-polyester-ceramic-composites-enhances-osteoinductivity
#8
Soheila Ali Akbari Ghavimi, Rama Rao Tata, Andrew J Greenwald, Brittany N Allen, David A Grant, Sheila A Grant, Mark W Lee, Bret D Ulery
Due to the growing number of patients suffering from musculoskeletal defects and the limited supply of and sub-optimal outcomes associated with biological graft materials, novel biomaterials must be created that can function as graft substitutes. For bone regeneration, composite materials that mimic the organic and inorganic phases of natural bone can provide cues which expedite and enhance endogenous repair. Specifically, recent research has shown that calcium and phosphate ions are inherently osteoinductive, so controllably delivering their release holds significant promise for this field...
May 11, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28495090/separation-of-ten-phosphorylated-mono-and-disaccharides-using-hilic-and-ion-pairing-interactions
#9
Caroline Mathon, Gregory A Barding, Cynthia K Larive
Phosphorylated carbohydrates are indispensable cogs in several key metabolic wheels for all forms of life. Here, a straightforward liquid chromatography method coupled to mass spectrometry detection was developed for phosphorylated sugars. For separation of the targeted compounds, hydrophilic interaction chromatography (HILIC) was used with a bridged-ethylene hybrid amide column under alkaline conditions using triethylamine as a mobile phase modifier. Methylphosphonic acid was added to the aqueous mobile phase to reduce the tailing of compounds containing phosphate groups, which are known to interact with stainless steel components of the separation system...
June 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28494462/in-vitro-growth-pattern-of-primary-human-osteoblasts-on-calcium-phosphate-and-polymethylmethacrylate-based-bone-cement
#10
Johannes Schauwecker, Mark Bock, Florian Pohlig, Heinz Mühlhofer, Jutta Tübel, Rüdiger von Eisenhart-Rothe, Chlodwig Kirchhoff
BACKGROUND/PURPOSE: Polymethylmethacrylate (PMMA) and calcium phosphate (Ca-P) cements are widely used for arthroplasty surgery and augmentation of bone defects. However, aseptic implant loosening in absence of wear-induced osteolysis indicates an unfavourable interaction between the cement surface and human osteoblasts. Our underlying hypothesis is that cement surfaces directly modify cell viability, proliferation rate, and cell differentiation. METHODS: To test this hypothesis, we examined primary human osteoblasts harvested from six individuals...
May 12, 2017: European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
https://www.readbyqxmd.com/read/28494141/metformin-induces-osteoblastic-differentiation-of-human-induced-pluripotent-stem-cell-derived-mesenchymal-stem-cells
#11
Ping Wang, Tao Ma, Dong Guo, Kevin Hu, Yan Shu, Hockin H K Xu, Abraham Schneider
Metformin, a first-line anti-diabetic drug used by millions of patients has been shown to have potential osteogenic properties. The present study was performed to test the hypothesis that clinically relevant doses of metformin promote the osteogenic differentiation and mineralization of induced Pluripotent Stem Cell-derived Mesenchymal Stem Cells (iPSC-MSCs). iPSC-MSCs were treated with metformin (10 μM) to assess cell viability, osteogenic differentiation, mineralization, and activation of the LKB1/AMP-activated protein kinase (AMPK) signaling pathway, a surrogate marker of metformin action...
May 11, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28493372/glycosuria-and-hyperglycemia-in-the-neonatal-period-as-the-first-clinical-sign-of-fanconi-bickel-syndrome
#12
María Pilar Bahíllo-Curieses, Rebeca Garrote-Molpeceres, María Miñambres-Rodríguez, M Rosa Del Real-Llorente, Cristina Tobar-Mideros, Sara Rellán-Rodríguez
Fanconi-Bickel syndrome is a rare inherited disease characterized by the combination of hepatorenal glycogen accumulation, proximal renal tubular dysfunction and impaired utilization of glucose and galactose. The first symptoms of the disorder are recognized in late infancy as clinical characteristics appear. Therapeutic approach is mainly conservative with supplements of calcium, phosphate and vitamin D and small frequent feedings to avoid hypoglycemia. We report 1 clinical case of very early diagnosis, a 19 days old baby girl, in which the first clinical sign of the disease was the detection of glycosuria and vomits...
May 11, 2017: Pediatric Diabetes
https://www.readbyqxmd.com/read/28492361/influence-of-rs2228570-on-transcriptional-activation-by-the-vitamin-d-receptor-in-human-gingival-fibroblasts-and-periodontal-ligament-cells
#13
Kaining Liu, Bing Han, Huanxin Meng, Jianxia Hou
BACKGROUND: Rs2228570 is the only known single nucleotide polymorphism of the vitamin D receptor (VDR) that alters the protein structure. VDRs can be distinguished using the restriction endonuclease FokI, and accordingly divided into three genotypes: FF, Ff and ff. The influence of rs2228570 on transcriptional activation by VDR in human gingival fibroblasts (hGFs) and periodontal ligament cells (hPDLCs) was investigated in this study. METHODS: hGFs and hPDLCs from 15 donors were cultured, genomic DNA was extracted, and genotypes were determined using the PCR-RFLP method...
May 11, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28491910/social-disadvantage-is-associated-with-lower-vitamin-d-levels-in-older-people-and-there-is-no-surrogate-for-its-measurement
#14
Adrian H Heald, Simon G Anderson, Jonathan J Scargill, Andrea Short, David Holland, Adnan Khan, Anthony A Fryer, Rachelle P Donn, Mark Livingston
Introduction: There is increasing evidence concerning adverse health consequences of low vitamin D levels. We determined whether there is any surrogate for measuring vitamin D in people older than 70 years and the relation between index of multiple deprivation (IMD) and vitamin D levels. Methods: Blood samples from 241 patients were included in this analysis. Concurrent measurements for 25-hydroxyvitamin D (25(OH)D), parathyroid hormone (PTH), and bone profile are reported. Results: The prevalence of total vitamin D insufficiency/deficiency (defined as total vitamin D <50 nmol/L) was 57...
January 2017: Gerontology & Geriatric Medicine
https://www.readbyqxmd.com/read/28487174/characterization-of-biochar-prepared-from-biogas-digestate
#15
Chao-Yi Hung, Wen-Tien Tsai, Jie-Wei Chen, Yu-Quan Lin, Yuan-Ming Chang
In the study, the biogas digestate was evaluated as a potential feedstock for preparing biochars at a broad temperature range of 300-900°C. The physico-chemical and pore properties of the resulting biochars (denoted as SDBC, solid digestate biochar), including calorific value (higher heating value), surface area/pore volume/pore size distribution, true density, scanning electron microscopy - energy dispersive X-ray spectroscopy (SEM-EDS) and X-ray powder diffraction (XRD), were studied. It was found that the higher heating values of the SDBC products were on a decreasing trend as pyrolysis temperature increased, but they indicated an increase in true density...
May 6, 2017: Waste Management
https://www.readbyqxmd.com/read/28485099/ectopic-bone-formation-by-aggregated-mesenchymal-stem-cells-from-bone-marrow-and-adipose-tissue-a-comparative-study
#16
Eelco M Fennema, Laurent A H Tchang, Huipin Yuan, Clemens A van Blitterswijk, Ivan Martin, Arnaud Scherberich, Jan de Boer
Tissue engineered constructs (TECs) based on spheroids of bone marrow mesenchymal stromal cells (BM-MSCs) combined with calcium phosphate (CaP) micro-particles and enveloped in a platelet-rich-plasma (PRP) hydrogel showed that aggregation of MSCs improves their ectopic bone formation potential. The stromal vascular fraction (SVF) and adipose-derived MSCs (ASCs) have been recognized as interesting MSC source for bone tissue engineering but their ectopic bone formation is limited. We investigated whether aggregation of ASCs could similarly improve ectopic bone formation by ASCs and SVF cells...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28479728/in-vitro-toxicity-and-genotoxic-activity-of-aqueous-leaf-extracts-from-four-varieties-of-olea-europea-l
#17
Luc Verschaeve, Hayet Edziri, Roel Anthonissen, Dalenda Boujnah, Fethia Skhiri, Hechmi Chehab, Mahjoub Aouni, Maha Mastouri
AIM: Despite its therapeutic value almost nothing is known about potential adverse health effects of Olea europea L. We therefore investigated the in vitro toxicity and genotoxicity of leaf extracts of this plant. MATERIAL AND METHODS: Extracts from olive tree leaves were obtained from four different regions in Tunisia. We investigated the in vitro toxicity, genotoxicity and antigenotoxicity of their aqueous extracts using the neutral red (NR) uptake, Vitotox and alkaline comet assays...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28479727/exploratory-studies-of-epicatechin-a-bioactive-compound-of-phyllanthus-niruri-on-the-antioxidant-enzymes-and-oxidative-stress-markers-in-d-galactosamine-induced-hepatitis-in-rats-a-study-with-reference-to-clinical-prospective
#18
Bhasha Shanmugam, Kondeti Ramudu Shanmugam, Sahukari Ravi, Ganjikunta Venkata Subbaiah, Chilakala Ramakrishana, Korivi Mallikarjuna, Kesireddy Sathyavelu Reddy
BACKGROUND: Hepatitis is a health problem affecting millions of people worldwide and it is the major risk factor for liver cirrhosis. In India, many plants are used to treat hepatitis. But little is known about the effects of (-)-epicatechin a bioactive compound of Phyllanthus niruri (PN) in hepatitis rats. OBJECTIVE: The present study was designed to explore the antioxidant property of (-)-epicatechin isolated from PN in D-Galactosamine (D-GalN) induced hepatitis rats...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28473963/rank-ligand-and-osteoprotegerin-as-biomarkers-in-the-differentiation-between-periprosthetic-joint-infection-and-aseptic-prosthesis-loosening
#19
Max J Friedrich, Matthias D Wimmer, Jan Schmolders, Andreas C Strauss, Milena M Ploeger, Hendrik Kohlhof, Dieter C Wirtz, Sascha Gravius, Thomas M Randau
AIM: To assess serum levels of RANK-ligand (RANKL) and osteoprotegerin (OPG) as biomarkers for periprosthetic joint infection (PJI) and compare their accuracy with standard tests. METHODS: One hundred and twenty patients presenting with a painful total knee or hip arthroplasty with indication for surgical revision were included in this prospective clinical trial. Based on standard diagnostics (joint aspirate, microbiological, and histological samples) and Musculoskeletal Infection Society consensus classification, patients were categorized into PJI, aseptic loosening, and control groups...
April 18, 2017: World Journal of Orthopedics
https://www.readbyqxmd.com/read/28472750/effect-of-the-presence-of-cholesterol-in-the-interfacial-microenvironment-on-the-modulation-of-the-alkaline-phosphatase-activity-during-in-vitro-mineralization
#20
B F Favarin, M A R Andrade, M Bolean, A M S Simão, A P Ramos, M F Hoylaerts, J L Millán, P Ciancaglini
Mineralization of the skeleton starts within cell-derived matrix vesicles (MVs); then, minerals propagate to the extracellular collagenous matrix. Tissue-nonspecific alkaline phosphatase (TNAP) degrades inorganic pyrophosphate (PPi), a potent inhibitor of mineralization, and contributes Pi (Phosphate) from ATP to initiate mineralization. Compared to the plasma membrane, MVs are rich in Cholesterol (Chol) (∼32%) and TNAP, but how Chol influences TNAP activity remains unclear. We have reconstituted TNAP in liposomes of dipalmitoylphosphatidylcholine (DPPC) or dioleoylphosphatidylcholine (DOPC) combined with Chol or its derivatives Cholestenone (Achol) and Ergosterol (Ergo)...
April 26, 2017: Colloids and Surfaces. B, Biointerfaces
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