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https://www.readbyqxmd.com/read/29156554/a-high-performance-liquid-chromatography-assay-method-for-the-determination-of-lidocaine-in-human-serum
#1
Hamdah M Al Nebaihi, Matthew Primrose, James S Green, Dion R Brocks
Here we report on the development of a selective and sensitive high-performance liquid chromatographic method for the determination of lidocaine in human serum. The extraction of lidocaine and procainamide (internal standard) from serum (0.25 mL) was achieved using diethyl ether under alkaline conditions. After liquid-liquid extraction, the separation of analytes was accomplished using reverse phase extraction. The mobile phase, a combination of acetonitrile and monobasic potassium phosphate, was pumped isocratically through a C18 analytical column...
November 18, 2017: Pharmaceutics
https://www.readbyqxmd.com/read/29150502/hyperthermophilic-carbamate-kinase-stability-and-anabolic-in-vitro-activity-at-alkaline-ph
#2
James E Hennessy, Melissa J Latter, Somayeh Fazelinejad, Amy Philbrook, Daniel M Bartkus, Hye-Kyung Kim, Hideki Onagi, John G Oakeshott, Colin Scott, Apostolos Alissandratos, Christopher J Easton
Carbamate kinases catalyze the conversion of carbamate to carbamoyl phosphate, which is readily transformed into other compounds. Carbamate forms spontaneously from ammonia and carbon dioxide in aqueous solutions, so the kinases have potential for sequestrative utilization of the latter compounds. Here, we compare seven carbamate kinases from mesophilic, thermophilic and hyperthermophilic sources. In addition to the known enzymes from Enterococcus faecalis and Pyrococcus furiosus, the previously unreported enzymes from the hyperthermophiles Thermococcus sibiricus and Thermococcus barophilus, the thermophiles Fervidobacterium nodosum and Thermosipho melanesiensis, and the mesophile Clostridium tetani, were all expressed recombinantly, each in high yield...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29146232/parathyroidectomy-in-patients-with-chronic-kidney-disease-impacts-of-different-techniques-on-the-biochemical-and-clinical-evolution-of-secondary-hyperparathyroidism
#3
Roxana de Fátima Camelo Albuquerque, Cinthia Esbrile Moraes Carbonara, Rita de Cássia T Martin, Luciene Machado Dos Reis, Climério Pereira do Nascimento, Sérgio Samir Arap, Rosa M A Moysés, Vanda Jorgetti, Fábio L M Montenegro, Rodrigo Bueno de Oliveira
BACKGROUND: Parathyroidectomy (PTx) decreases the mortality rate of refractory secondary hyperparathyroidism (rSHP) due to chronic kidney disease. A consensus regarding which techniques of PTx are associated with better outcomes is not available. The aims of this study are to evaluate the clinical and laboratory evolution of 49 hemodialysis patients with rSHP who underwent PTx using different techniques. METHODS: Patients underwent subtotal PTx (sub-PTx) or total PTx with autotransplantation (AT) of 45 (PTx-AT45) or 90 parathyroid fragments (PTx-AT90) and were followed for 12 months...
November 13, 2017: Surgery
https://www.readbyqxmd.com/read/29142975/sclerostin%C3%A2-a-debutant-on-the-autosomal-dominant-polycystic-kidney-disease-scene
#4
Magdalena Jankowska, Mathias Haarhaus, Abdul Rashid Qureshi, Bengt Lindholm, Pieter Evenepoel, Peter Stenvinkel
Introduction: Autosomal dominant polycystic kidney disease (ADPKD) is a genetic disease originating from a mutation in genes encoding polycystin 1 and 2. Recent evidence suggests that these polycystins mediate mechanosensation not only in the primary cilium of kidney cells but also in bone cells. The Wnt/β-catenin signaling pathway plays a central role in mechanotransduction in osteocytes. Mechanical unloading causes the upregulation of the Wnt inhibitor sclerostin. We tested the hypothesis that ADPKD associates with higher circulating sclerostin levels...
May 2017: KI Reports
https://www.readbyqxmd.com/read/29141319/-homozygous-ectonucleotide-pyrophosphatase-phosphodiesterase-1-variants-in-a-girl-with-hypophosphatemic-rickets-and-literature-review
#5
Z Q Liu, X B Chen, F Y Song, K Gao, M F Qiu, Y Qian, M Du
Objective: To investigate the clinical features and genetic characteristics of patients with ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) gene variants. Method: The clinical data of a patient with ENPP1 homozygous variants from Capital Institute of Pediatrics was collected, the related literature was searched from China National Knowledge Infrastructure, Wanfang Data Knowledge Service Platform, National Center from Biotechnology Information and PubMed by using search term "ENPP1" , "hypophosphatemic rickets" ...
November 2, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/29140371/biofunctionalization-of-titanium-granules-with-simvastatin-for-improving-osteogenic-activity-and-antibacterial-properties-ex-vivo-study
#6
Zahra Gorgin Karaji, Behzad Houshmand, Shahsanam Abbasi, Sara Shafiei, Shahab Faghihi
PURPOSE: Titanium-based biomaterials present good biocompatibility, while their osseointegration and antibacterial properties need to be improved. This study aimed to enhance the bone-bonding ability of titanium-based granules, which are intended to be used as bone graft. MATERIALS AND METHODS: The titanium granules were anodized in ethylene glycol-based electrolyte and subsequently annealed to be loaded separately with simvastatin. The samples were then inspected with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) for drug loading...
November 2017: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/29137892/role-of-pyrophosphate-in-vascular-calcification-in-chronic-kidney-disease
#7
Daniel Azpiazu, Sergio Gonzalo, Emilio González-Parra, Jesús Egido, Ricardo Villa-Bellosta
Vascular calcification is a pathology characterized by the deposition of calcium-phosphate in cardiovascular structures, mainly in the form of hydroxyapatite crystals, resulting in ectopic calcification. It is correlated with increased risk of cardiovascular disease and myocardial infarction in diabetic patients and in those with chronic kidney disease (CKD). Vascular smooth muscle cells are sensitive to changes in inorganic phosphate (Pi) levels. They are able to adapt and modify some of their functions and promote changes which trigger calcification...
November 11, 2017: Nefrología: Publicación Oficial de la Sociedad Española Nefrologia
https://www.readbyqxmd.com/read/29137454/determination-of-cancer-cell-based-ph-sensitive-fluorescent-carbon-nanoparticles-of-cross-linked-polydopamine-by-fluorescence-sensing-of-alkaline-phosphatase-activity-on-coated-surfaces-and-aqueous-solution
#8
Eun Bi Kang, Cheong A Choi, Zihnil Adha Islamy Mazrad, Sung Han Kim, Insik In, Sung Young Park
The tumor-specific sensitive fluorescence sensing of cellular alkaline phosphatase (ALP) activity based on host-guest specific and pH sensitive by ere conducted on coated surfaces and aqueous states. Cross-linked fluorescent nanoparticles (C-FNP) consisting of β-cyclodextrin (β-CD)/boronic acid (BA) and fluorescent hyaluronic acid [FNP(HA)] were conjugated to fluorescent polydopamine [FNP(pDA)]. To determine the quenching effect of this system, hydrolysis of 4-nitrophenyl phosphate (NPP) to 4-nitrophenol (NP) was performed in the cavity of β-CD in the presence of ALP activated photo-induced electron transfer (PET) between NP and C-FNP...
November 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29136933/alkaline-solubilization-of-excess-mixed-sludge-and-the-recovery-of-released-phosphorus-as-magnesium-ammonium-phosphate
#9
De-Chao Xu, Cong-Qiang Zhong, Kui-Hao Yin, Sheng-Hua Peng, Ting-Ting Zhu, Gong Cheng
The alkaline solubilization of excess mixed sludge was investigated and subsequently the released phosphorus was recovered as magnesium ammonium phosphate (MAP). Considerable and rapid release of glycogen and protein was encountered after alkaline addition into the sludge. Only 45.0% of sludge cells were destructed after 240 min treatment in R1 while the corresponding ratio was 96.1% in R3 according to the release of DNA. Non-apatite inorganic phosphorus (NAIP) in the alkaline treated sludge decreased due to the dissolution of Al(OH)3 and AlPO4, which was the dominant reason for phosphorus release...
October 26, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29136753/-metabolomics-study-of-tris-2-chloroethyl-phosphate-induced-hepaotoxicity-and-nephrotoxicity-in-sprague-dawley-rats
#10
W Q Yang, F Zhao, L Li, Y J Fang
Objective: To discuss the potential toxic target organ and the toxic effects and mechanisms of tris (2-chloroethyl) phosphate (TCEP) on SD rats. Methods: 40 female SD rats weaning from milk for 21 days, weighted (50±2.3)g were selected as subjects and marked by the weight. They were randomly divided into 4 groups, namely control group, 50 (L), 100 (M) and 250 (H) mg·kg(-1)·d(-1) dose of TCEP group. Each group has 10 rats, and administrated the corresponding dose of drug or vehicle by mouth, quaque die for 60 days...
November 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29130550/-1-h-nmrs-of-carnosine-combined-with-31-p-nmrs-to-better-characterize-skeletal-muscle-ph-dysregulation-in-duchenne-muscular-dystrophy
#11
Harmen Reyngoudt, Suna Turk, Pierre G Carlier
In recent years, quantitative nuclear magnetic resonance imaging and spectroscopy (NMRI and NMRS) have been used more systematically as outcome measures in natural history and clinical trial studies for Duchenne muscular dystrophy (DMD). Whereas most of these studies have emphasized the evaluation of the fat fraction as an assessment for disease severity, less focus has been placed on metabolic indices measured by NMRS. (31) P NMRS in DMD reveals an alkaline inorganic phosphate (Pi ) pool, originating from either leaky dystrophic myocytes or an increased interstitial space...
November 12, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/29130461/intracellular-coassembly-boosts-the-anti-inflammation-capacity-of-dexamethasone
#12
Wei Tang, Jingbo Yang, Zhibin Zhao, Zhexiong Lian, Gaolin Liang
Dexamethasone (Dex) is one of the essential medicines used to treat inflammation diseases but an overdose of Dex leads to severe adverse effects. The development of a new strategy to boost the anti-inflammation efficacy of Dex is, therefore, important but remains challenging. Herein, by employing an enzyme-instructed self-assembly system, we developed an intracellular coassembly strategy to boost the anti-inflammation efficacy of Dex. Under the catalysis of alkaline phosphatase (ALP), the hydrogelator precursor Nap-Phe-Phe-Tyr(H2PO3)-OH (1p) self-assembled to form Gel 1 but dexamethasone sodium phosphate (Dp) only yielded Dex precipitates...
November 13, 2017: Nanoscale
https://www.readbyqxmd.com/read/29114228/integrative-temporo-spatial-mineralogic-spectroscopic-and-proteomic-analysis-of-postnatal-enamel-development-in-teeth-with-limited-growth
#13
Mirali Pandya, Hui Liu, Smit J Dangaria, Weiying Zhu, Leo L Li, Shuang Pan, Moufida Abufarwa, Roderick G Davis, Stephen Guggenheim, Timothy Keiderling, Xianghong Luan, Thomas G H Diekwisch
Tooth amelogenesis is a complex process beginning with enamel organ cell differentiation and enamel matrix secretion, transitioning through changes in ameloblast polarity, cytoskeletal, and matrix organization, that affects crucial biomineralization events such as mineral nucleation, enamel crystal growth, and enamel prism organization. Here we have harvested the enamel organ including the pliable enamel matrix of postnatal first mandibular mouse molars during the first 8 days of tooth enamel development to conduct a step-wise cross-sectional analysis of the changes in the mineral and protein phase...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29112902/development-of-a-stability-indicating-hplc-method-for-simultaneous-determination-of-ten-related-substances-in-vonoprazan-fumarate-drug-substance
#14
Zhiqiang Luo, Aoxue Liu, Yang Liu, Guopeng Wang, Xinjing Chen, Hao Wang, Mengwei Li, Haili Zhang, Yanhua Qiu, Huaqiang Zhai
Vonoprazan fumarate is a novel potassium-competitive acid blocker for the treatment of acid-related diseases. In the present study, a simple, fast, and economic reversed-phase liquid chromatography (LC) method was developed for the analysis of ten related substances (raw materials, by-products and degradants) in vonoprazan fumarate. The optimized separation was performed on a Phenomenex Kinetex EVO C18 (250mm×4.6mm, 5.0μm) column. The mobile phase consisted of (A) 0.03M sodium phosphate buffer (pH adjusted to 6...
November 4, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29111484/self-calibrated-fluorescent-probe-resembled-as-an-indicator-of-the-lysosomal-phosphatase-pertaining-to-the-cancer-cells
#15
Arup Podder, Susan M Alex, Mrinmoy Maiti, Kaustabh Kumar Maiti, Sankarprasad Bhuniya
A self-calibrated fluorescent probe Lyso-Phos has synthesized followed by a straightforward synthetic pathway. Lyso-Phos acts as an indicator for lysosomal phosphatase. Its photophysical property including cellular imaging was described. Lyso-Phos showed ratiometric UV-Vis- absorption changes from λabs 370nm to λabs 450nm in the presence of alkaline phosphatase (ALP). On the other hand, fluorescence intensity λem 560nm of Lyso-Phos has increased around 45-fold in the presence of ALP. The probe Lyso-Phos was found to be highly chemoselective toward the phosphatase compared with other ubiquitous entities in cellular milieu...
October 18, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29111354/three-gpi-anchored-alkaline-phosphatases-are-involved-in-the-intoxication-of-cry1ca-toxin-to-spodoptera-exigua-larvae
#16
Xiang-Liang Ren, Hong-Yan Hu, Wei-Li Jiang, Xiao-Yan Ma, Ya-Jie Ma, Guo-Qing Li, Yan Ma
Glycosylphosphatidylinositol (GPI)-anchored alkaline phosphatase (ALP) isoforms are crucial for the intoxication of crystal proteins (Cry) to several insect species. We cloned five SeALPs from the larval midgut of Spodoptera exigua, a major pest of many crops. All five SeALPs contain a signal peptide at the N-terminus, a phosphatase domain, and a GPI-anchor site at the C-terminus. Additionally, the sequences encode two or three potential N-glycosylation sites. The five SeALPs were highly expressed at the larval stage, especially in the larval gut or Malpighian tubules...
October 27, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29104854/chirality-controls-reaction-diffusion-of-nanoparticles-for-inhibiting-cancer-cells
#17
Xuewen Du, Jie Zhou, Jiaqing Wang, Rong Zhou, Bing Xu
Reaction-diffusion (RD) is the most important inherent feature of living organism, but it has yet to be used for developing biofunctional nanoparticles (NPs). Here we show the use of chirality to control the RD of NPs for selectively inhibiting cancer cells. We observe that L-phosphotyrosine (L-pY) decorated NPs (NP@L-pYs) are innocuous to cells, but D-pY decorated ones (NP@D-pYs) selectively inhibit cancer cells. Our study shows that alkaline phosphatases (ALP), presented in the culture and overexpressed on the cancer cells, dephosphorylates NP@L-pYs much faster than NP@D-pYs...
January 2017: ChemNanoMat: Chemistry of Nanomaterials for Energy, Biology and More
https://www.readbyqxmd.com/read/29099939/pth-1-34-for-surgical-hypoparathyroidism-a-2-year-prospective-open-label-investigation-of-efficacy-and-quality-of-life
#18
Andrea Palermo, Assunta Santonati, Gaia Tabacco, Daniela Bosco, Antonio Spada, Claudio Pedone, Bruno Raggiunti, Tina Doris, Daria Maggi, Franco Grimaldi, Sivlia Manfrini, Fabio Vescini
Context: Daily PTH(1-34) administrations can reduce the required total daily dose of calcium/calcitriol and restore normocalcemia in refractory hypoparathyroidism. However, most of the PTH(1-34) trials were conducted on small cohorts including subjects with hypoparathyroidism of various etiologies and quality of life(QOL) was not investigated. Objective: To investigate the effects of 24 month PTH(1-34) treatment in a homogeneous cohort of adult subjects with postoperative hypoparathyroidism and to evaluate quality of life(QOL) changes...
November 1, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29098411/distinguishing-between-metabolically-active-and-dormant-bacteria-on-paper
#19
Stephanie A Hice, Miguel C Santoscoy, Michelle L Soupir, Rebecca Cademartiri
Switching between metabolically active and dormant states provides bacteria with protection from environmental stresses and allows rapid growth under favorable conditions. This rapid growth can be detrimental to the environment, e.g., pathogens in recreational lakes, or to industrial processes, e.g., fermentation, making it useful to quickly determine when the ratio of dormant to metabolically active bacteria changes. While a rapid increase in metabolically active bacteria can cause complications, a high number of dormant bacteria can also be problematic, since they can be more virulent and antibiotic-resistant...
November 2, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29096595/pleiotropic-actions-of-fgf23
#20
Reinhold G Erben
Fibroblast growth factor-23 (FGF23) is a bone-derived hormone, mainly produced by osteoblasts and osteocytes in response to increased extracellular phosphate and circulating vitamin D hormone. Endocrine FGF23 signaling requires co-expression of the ubiquitously expressed FGF receptor 1 (FGFR1) and the co-receptor α-Klotho (Klotho). In proximal renal tubules, FGF23 suppresses the membrane expression of the sodium-phosphate cotransporters Npt2a and Npt2c which mediate urinary reabsorption of filtered phosphate...
January 1, 2017: Toxicologic Pathology
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