keyword
MENU ▼
Read by QxMD icon Read
search

Ctcs

keyword
https://www.readbyqxmd.com/read/28925689/highly-efficient-capture-and-electrochemical-release-of-circulating-tumor-cells-by-using-aptamers-modified-gold-nanowire-arrays
#1
Ting-Ting Zhai, Dekai Ye, Qian-Wen Zhang, Zeng-Qiang Wu, Xing-Hua Xia
The effectively capturing and releasing of circulating tumor cells (CTCs) is of significant importance in cancer prognose and treatment. Herein, we report a highly efficient method to capture and release human leukemic lymphoblasts (CCRF-CEM) using aptamers modified gold nanowire arrays (AuNWs). The gold nanowires, showing tunable morphologies from relatively random pillar deposit to uniform arrays, were fabricated by electrochemical deposition using anodic aluminum oxide (AAO) as template. Upon simply modification with aptamers by Au-S chemistry, the uniform AuNWs exhibit higher specificity to target cells...
September 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28919743/nanovelcro-captured-ctc-number-concomitant-with-enhanced-serum-levels-of-mmp7-and-mmp9-enables-accurate-prediction-of-metastasis-and-poor-prognosis-in-patients-with-lung-adenocarcinoma
#2
Yu Sun, Yangshan Chen, Shuhua Li, Yiyan Lei, Di Xu, Neng Jiang, Yang Zhang, Jessica Cao, Zunfu Ke
Lung adenocarcinoma (LADC) is among the most malignant cancers that frequently develops micrometastases even in early stages of the disease. Circulating tumor cell (CTC) number, matrix metalloproteinase (MMP) 7, and MMP9 show great prospects as predictive biomarkers in many tumors. However, the interactions between these biomarkers and the molecular basis of their roles in the metastasis and prognosis of LADC remain unclear. The present study revealed that an elevated CTC count and overexpression of MMP7 and MMP9 correlate with metastasis and clinical progression in LADC patients (n=143)...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28919351/suspension-state-increases-reattachment-of-breast-cancer-cells-by-up-regulating-lamin-a-c
#3
Xiaomei Zhang, Yonggang Lv
Extravasation is a rate-limiting step of tumor metastasis, for which adhesion to endothelium of circulating tumor cells (CTCs) is the prerequisite. The suspension state of CTCs undergoing detachment from primary tumor is a persistent biomechanical cue, which potentially regulates the biophysical characteristics and cellular behaviors of tumor cells. In this study, breast tumor cells MDA-MB-231 in suspension culture condition were used to investigate the effect of suspension state on reattachment of CTCs. Our study demonstrated that suspension state significantly increased the adhesion ability of breast tumor cells...
September 14, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28918054/rapid-and-sensitive-detection-of-breast-cancer-cells-in-patient-blood-with-nuclease-activated-probe-technology
#4
Sven Kruspe, David D Dickey, Kevin T Urak, Giselle N Blanco, Matthew J Miller, Karen C Clark, Elliot Burghardt, Wade R Gutierrez, Sneha D Phadke, Sukriti Kamboj, Timothy Ginader, Brian J Smith, Sarah K Grimm, James Schappet, Howard Ozer, Alexandra Thomas, James O McNamara, Carlos H Chan, Paloma H Giangrande
A challenge for circulating tumor cell (CTC)-based diagnostics is the development of simple and inexpensive methods that reliably detect the diverse cells that make up CTCs. CTC-derived nucleases are one category of proteins that could be exploited to meet this challenge. Advantages of nucleases as CTC biomarkers include: (1) their elevated expression in many cancer cells, including cells implicated in metastasis that have undergone epithelial-to-mesenchymal transition; and (2) their enzymatic activity, which can be exploited for signal amplification in detection methods...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28916277/circulating-tumor-cell-as-a-biomarker-for-evaluating-allogenic-nk-cell-immunotherapy-on-stage-iv-non-small-cell-lung-cancer
#5
Mao Lin, Shu-Zhen Liang, Jian Shi, Li-Zhi Niu, Ji-Bing Chen, Ming-Jie Zhang, Ke-Cheng Xu
In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-NK in patients with stage IV non- small cell lung cancer (NSCLC) as a reference for understanding the relevance of any changes to the efficacy of NK cells therapy. The patients were given one to three courses of immunotherapy. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 31 patients with stage IV NSCLC at 1day before and 7 and 30d after NK cells therapy using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR)...
September 12, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28911924/sialyl-tn-identifies-muscle-invasive-bladder-cancer-basal-and-luminal-subtypes-facing-decreased-survival-being-expressed-by-circulating-tumor-cells-and-metastases
#6
Luís Lima, Manuel Neves, Marta I Oliveira, Lorena Dieguez, Rui Freitas, Rita Azevedo, Cristiana Gaiteiro, Janine Soares, Dylan Ferreira, Andreia Peixoto, Elisabete Fernandes, Diana Montezuma, Ana Tavares, Ricardo Ribeiro, Ana Castro, Manuel Oliveira, Avelino Fraga, Celso A Reis, Lúcio Lara Santos, José Alexandre Ferreira
OBJECTIVES: To evaluate the potential of sialyl-Tn (STn), a cancer-associated glycan antigen present in membrane glycoproteins, to improve a recent molecular model for stratification and prognostication of advanced stage bladder tumors based on keratins (KRT14, 5, and 20) expression. In addition, determine the association between STn and disease dissemination based on the evaluation of circulating tumor cells (CTCs) and the metastasis, which is a critical matter to improve patient management...
September 11, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/28904890/biophysical-technologies-for-understanding-circulating-tumor-cell-biology-and-metastasis
#7
REVIEW
Derrick W Su, Jorge Nieva
An understanding of cancer evolution in lung cancer with its associated resistance to therapy can only be achieved with repeated sampling and analysis of the cancer. Given the high risks and costs associated with repeat physical biopsy, alternative technologies must be applied. Several modalities exist for analysis and re-analysis of cancer biology. Among them are the CellSearch platform, the CTC chip, and the high-definition CTC platform. While the former is primarily able to provide prognosticating information in the form of CTC enumeration, the latter two have the advantage of serving as a platform to study tumor biology...
August 2017: Translational Lung Cancer Research
https://www.readbyqxmd.com/read/28904889/challenges-and-unanswered-questions-for-the-next-decade-of-circulating-tumour-cell-research-in-lung-cancer
#8
REVIEW
Sumitra Mohan, Francesca Chemi, Ged Brady
Since blood borne circulating tumour cells (CTCs) initially shed from the primary tumour can seed and initiate metastasis at distant sites a better understanding of the biology of CTCs and their dissemination could provide valuable information that could guide therapeutic intervention and real time monitoring of disease progression. Although CTC enumeration has provided a reliable prognostic readout for a number of cancers, including lung cancer, the precise clinical utility of CTCs remains to be established...
August 2017: Translational Lung Cancer Research
https://www.readbyqxmd.com/read/28904888/routine-clinical-use-of-circulating-tumor-cells-for-diagnosis-of-mutations-and-chromosomal-rearrangements-in-non-small-cell-lung-cancer-ready-for-prime-time
#9
REVIEW
Emma Pailler, Vincent Faugeroux, Marianne Oulhen, Cyril Catelain, Françoise Farace
In non-small cell lung cancer (NSCLC), diagnosis of predictive biomarkers for targeted therapies is currently done in small tumor biopsies. However, tumor biopsies can be invasive, in some cases associated with risk, and tissue adequacy, both in terms of quantity and quality is often insufficient. The development of efficient and non-invasive methods to identify genetic alterations is a key challenge which circulating tumor cells (CTCs) have the potential to be exploited for. CTCs are extremely rare and phenotypically diverse, two characteristics that impose technical challenges and impact the success of robust molecular analysis...
August 2017: Translational Lung Cancer Research
https://www.readbyqxmd.com/read/28904887/biology-and-clinical-significance-of-circulating-tumor-cell-subpopulations-in-lung-cancer
#10
REVIEW
Linda O'Flaherty, Harriet Wikman, Klaus Pantel
By identifying and tracking genetic changes in primary tumors and metastases, patients can be stratified for the most efficient therapeutic regimen by screening for known biomarkers. However, retrieving tissues biopsies is not always feasible due to tumor location or risk to patient. Therefore, a liquid biopsies approach offers an appealing solution to an otherwise invasive procedure. The rapid growth of the liquid biopsy field has been aided by improvements in the sensitivity and specificity of enrichment assays for isolating circulating tumor cells (CTCs) from normal surrounding blood cells...
August 2017: Translational Lung Cancer Research
https://www.readbyqxmd.com/read/28904886/circulating-tumor-cell-interactions-with-macrophages-implications-for-biology-and-treatment
#11
REVIEW
Gerhard Hamilton, Barbara Rath
Cancer and metastasis are closely associated with inflammation. Macrophages are important effector cells in enhancing tumor proliferation, invasion and providing protection against the immune system. Despite advanced knowledge of tumor-macrophage interactions, the role of macrophages in emergence and invasion of circulating tumor cells (CTCs) is not known. A series of six CTC cell lines have been derived from blood of patients with extensive disease small cell lung cancer (ED-SCLC) in our lab, most likely representing a homogenous cell population of the actual metastasis-initiating cells (MIC) of CTCs...
August 2017: Translational Lung Cancer Research
https://www.readbyqxmd.com/read/28904885/the-clinical-utility-of-circulating-tumour-cells-in-patients-with-small-cell-lung-cancer
#12
REVIEW
Victoria Foy, Fabiola Fernandez-Gutierrez, Corinne Faivre-Finn, Caroline Dive, Fiona Blackhall
Small cell lung cancer (SCLC) accounts for 15% of lung cancer diagnosed worldwide. It is aggressive and characterised by early metastatic spread with rapid development of chemo resistance such that less than 5% of patients diagnosed survive 5 years. Surgery is rarely performed and failure to identify new effective treatments has been attributed in a large part to lack of good quality tumour biopsies available for translational research. Liquid biopsies provide a minimally invasive alternative to traditional tumour biopsy...
August 2017: Translational Lung Cancer Research
https://www.readbyqxmd.com/read/28904884/circulating-tumor-cells-and-cdx-models-as-a-tool-for-preclinical-drug-development
#13
REVIEW
Alice Lallo, Maximilian W Schenk, Kristopher K Frese, Fiona Blackhall, Caroline Dive
Lung cancers are the main cause of cancer-related deaths worldwide. Efforts placed to improve the survival of lung cancer patients and untangle the complexity of this disease, have resulted in the generation of hundreds of lung cancer cell lines and several genetically engineered mouse models (GEMMs). Although these research tools have extended our knowledge of lung cancer, improvement in the clinical care of lung cancer patients have been limited overall, with measured optimism regarding initial responses to targeted therapies in stratified subgroups of patients...
August 2017: Translational Lung Cancer Research
https://www.readbyqxmd.com/read/28903450/clinical-and-biological-significance-of-circulating-tumor-cells-circulating-tumor-dna-and-exosomes-as-biomarkers-in-colorectal-cancer
#14
REVIEW
Shiyu Jia, Rui Zhang, Ziyang Li, Jinming Li
Colorectal cancer (CRC) has been the fourth leading cause of cancer-related mortality worldwide. Owing to clonal evolution and selection, CRC treatment needs multimodal therapeutic approaches and due monitoring of tumor progression and therapeutic efficacy. Liquid biopsy, involving the use of circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), and exosomes, may offer a promising noninvasive alternative for diagnosis and for real-time monitoring of tumor evolution and therapeutic response compared to traditional tissue biopsy...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28903376/improving-circulating-tumor-cells-enumeration-and-characterization-to-predict-outcome-in-first-line-chemotherapy-mcrpc-patients
#15
Luis León-Mateos, Helena Casas, Alicia Abalo, María Vieito, Manuel Abreu, Urbano Anido, Antonio Gómez-Tato, Rafael López, Miguel Abal, Laura Muinelo-Romay
INTRODUCTION: There is a critical need of new surrogate markers for improving the therapeutic selection and monitoring of metastatic prostate cancer patients. Nowadays clinical management of these patients is been driven by biochemical and clinical parameters without enough accuracy to allow a real personalized medicine. The present study was conducted to go insight the molecular profile of circulating tumor cells (CTCs) isolated from advanced metastatic castration-resistant prostate cancer (mCRPC) with the aim of identifying prognostic marker with potential utility for therapy selection and monitoring...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28900503/label-free-rapid-viable-enrichment-of-circulating-tumor-cell-by-photosensitive-polymer-based-microfilter-device
#16
Yoon-Tae Kang, Il Doh, Jiyoung Byun, Hee Jin Chang, Young-Ho Cho
We present a clinical device for simple, rapid, and viable isolation of circulating tumor cells (CTCs) from cancer patient bloods. In spite of the clinical importance of CTCs, the lack of easy and non-biased isolation methods is a big hurdle for implementing CTC into clinical use. The present device made of photosensitive polymer was designed to attach to conventional syringe to isolate the CTCs at minimal resources. Its unique tapered-slits on the filter are capable not only to isolate the cell based on their size and deformability, but also to increase sample flow rate, thus achieving label-free rapid viable CTC isolation...
2017: Theranostics
https://www.readbyqxmd.com/read/28900219/rapid-staining-of-circulating-tumor-cells-in-three-dimensional-microwell-dialysis-3d-%C3%AE-dialysis-chip
#17
Wanying Cho, Rangadhar Pradhan, Hsin Ying Chen, Yi-Hsuan Weng, Hsueh Yao Chu, Fan-Gang Tseng, Chien-Ping Lin, Jeng-Kai Jiang
The conventional techniques to detect circulating tumour cells (CTCs) are lengthy and the use of centrifugal forces in this technique may cause cell mortality. As the number of CTCs in patients is quite low, the present study aims towards a gentler diagnostic procedure so as not to lose too many CTCs during the sample preparation process. Hence, a Three-Dimensional Microwell dialysis (3D-μDialysis) chip was designed in this study to perform gentle fluorescence-removal process by using dialysis-type flow processes without centrifuging...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28899718/molecular-characterization-of-circulating-tumor-cells-from-bench-to-bedside
#18
REVIEW
Yanmeng Li, Shaohan Wu, Fan Bai
Circulating tumor cells (CTCs) are cancer cells discovered in cancer patients' peripheral blood that successfully escape from the primary tumor site and/or metastases, struggle to survive in the bloodstream, and have potential for seeding metastases. Numerous methods have been proposed to capture CTCs. The value of CTCs as a means of understanding cancer metastasis and a major form of 'liquid biopsy' has been widely demonstrated. Recently, single-cell molecular analyses of CTCs have provided profound biological insights into tumor heterogeneity, mechanism of metastasis and tumor evolution...
September 9, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28895421/in-vivo-isolation-of-circulating-tumor-cells-in-non-small-cell-lung-cancer-patients-by-cellcollector
#19
L Gasiorowski, W Dyszkiewicz, P Zielinski
In non-small cell lung cancer (NSCLC) circulating tumor cells (CTCs) can provide information on patient prognosis and treatment efficacy. Currently CTCs are mostly isolated in vitro from small volumes of patient blood samples. The aim of the study was to assess a medical device for in vivo isolation of CTCs directly from the blood of NSCLC patients. The device was inserted in a cubital vein through a standard cannula for thirty minutes. The interaction of target CTCs with the CellCollector was mediated by an antibody directed against the epithelial cell adhesion molecule...
September 12, 2017: Neoplasma
https://www.readbyqxmd.com/read/28894383/comparison-of-autogeneic-and-allogeneic-natural-killer-cells-immunotherapy-on-the-clinical-outcome-of-recurrent-breast-cancer
#20
Shuzhen Liang, Kecheng Xu, Lizhi Niu, Xiaohua Wang, Yingqing Liang, Mingjie Zhang, Jibing Chen, Mao Lin
In the present study, we aimed to compare the clinical outcome of autogeneic and allogeneic natural killer (NK) cells immunotherapy for the treatment of recurrent breast cancer. Between July 2016 and February 2017, 36 patients who met the enrollment criteria were randomly assigned to two groups: autogeneic NK cells immunotherapy group (group I, n=18) and allogeneic NK cells immunotherapy group (group II, n=18). The clinical efficacy, quality of life, immune function, circulating tumor cell (CTC) level, and other related indicators were evaluated...
2017: OncoTargets and Therapy
keyword
keyword
34632
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"