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https://www.readbyqxmd.com/read/29770688/frosted-slides-decorated-with-silica-nanowires-for-detecting-circulating-tumor-cells-from-prostate-cancer-patients
#1
Haijun Cui, Binshuai Wang, Wenshuo Wang, Yuwei Hao, Chuanyong Liu, Kai Song, Shudong Zhang, Shutao Wang
Developing low-cost and highly efficient nano-biochips are important for liquid biopsies, real-time monitoring, and precision medicine. By in situ growth of silica nanowires on a commercial frosted slide, we develop a biochip for effective circulating tumor cells (CTCs) detection after modifying epithelial cell adhesion molecule antibody (anti-EpCAM). The biochip shows the specificity and high capture efficiency of 85.4 ± 8.3% for prostate cancer cell line (PC-3). The micro-sized frosted slides and silica nanowires allow enhanced efficiency in capture EpCAM positive cells by synergistic topographic interactions...
May 17, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29769179/rna-profiles-of-circulating-tumor-cells-and-extracellular-vesicles-for-therapy-stratification-of-metastatic-breast-cancer-patients
#2
Corinna Keup, Pawel Mach, Bahriye Aktas, Mitra Tewes, Hans-Christian Kolberg, Siegfried Hauch, Markus Sprenger-Haussels, Rainer Kimmig, Sabine Kasimir-Bauer
BACKGROUND: Liquid biopsies are discussed to provide surrogate markers for therapy stratification and monitoring. We compared messenger RNA (mRNA) profiles of circulating tumor cells (CTCs) and extracellular vesicles (EVs) in patients with metastatic breast cancer (MBC) to estimate their utility in therapy management. METHODS: Blood was collected from 35 hormone receptor-positive/HER2-negative patients with MBC at the time of disease progression and at 2 consecutive staging time points...
May 16, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29764586/-relationship-between-fgfr1-gene-regulation-of-circulating-tumor-cells-and-clinical-features-of-non-small-cell-lung-cancer
#3
Lei Liu, Cheng Huang, Li Li, Naixin Liang, Shanqing Li
BACKGROUND: The methods of detection for recurrence and metastasis in patients with non-small cell lung cancer (NSCLC) have hysteresis and one-sidedness. This study summarizes the relationship between the circulating tumor cell (CTC) in peripheral blood, expression of fibroblast growth factor receptor 1 (FGFR1) and clinic pathological features in 30 patients with NSCLC so as to provide new ideas for the detection of tumor recurrence and metastasis. METHODS: To analyze the clinical data and CTC detection data of 30 cases of NSCLC in Department of Thoracic Surgery, Peking Union Medical College Hospital from November 2016 to June 2017...
May 20, 2018: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
https://www.readbyqxmd.com/read/29762619/isolation-and-genome-sequencing-of-individual-circulating-tumor-cells-using-hydrogel-encapsulation-and-laser-capture-microdissection
#4
Emily S Park, Justin P Yan, Richard A Ang, Jeong Hyun Lee, Xiaoyan Deng, Simon P Duffy, Kevin Beja, Matti Annala, Peter C Black, Kim N Chi, Alexander W Wyatt, Hongshen Ma
Circulating tumor cells (CTCs) are malignant cells released into the bloodstream with the potential to form metastases in secondary sites. These cells, acquired non-invasively, represent a sample of highly relevant tumor tissue that is an alternative to difficult and low-yield tumor biopsies. In recent years, there has been growing interest in genomic profiling of CTCs to enable longitudinal monitoring of the tumor's adaptive response to therapy. However, due to their extreme rarity, genotyping CTCs has proved challenging...
May 15, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29759567/epcam-duality-becomes-this-molecule-in-a-new-dr-jekyll-and-mr-hyde-tale
#5
REVIEW
Alejandro Herreros-Pomares, Cristóbal Aguilar-Gallardo, Silvia Calabuig-Fariñas, Rafael Sirera, Eloísa Jantus-Lewintre, Carlos Camps
EpCAM, known as an epithelial cell adhesion molecule, plays an essential role in cell adhesion, migration, metastasis and cell signalling. Rather than acting as an apoptosis antagonist, it induces cellular proliferation that impacts the cell cycle, and as a signalling transducer it uses and enhances the Wnt pathway, which is significantly relevant in cell renewal and cancer. EpCAM has become a marker of circulating tumour cells (CTCs) in lung cancer due to its specificity, and its high and stable expression level...
June 2018: Critical Reviews in Oncology/hematology
https://www.readbyqxmd.com/read/29759554/the-identification-and-isolation-of-ctcs-a-biological-rubik-s-cube
#6
REVIEW
Cristina Mansilla, Elena Soria, Natalia Ramírez
Liquid biopsy represents an alternative to conventional biopsies for the evaluation of tumors mainly due to its easy sampling. One of the main applications is the enumeration of Circulating Tumor Cells (CTCs) to evaluate tumor progression or response to treatment. The analysis of the functional characteristics of CTCs could give us much more information about their role in order to establish a more personalized treatment for the patients. The major issue that has to be solved is the isolation of the CTC population...
June 2018: Critical Reviews in Oncology/hematology
https://www.readbyqxmd.com/read/29759193/highly-sensitive-detection-and-mutational-analysis-of-lung-cancer-circulating-tumor-cells-using-integrated-combined-immunomagnetic-beads-with-a-droplet-digital-pcr-chip
#7
Wanlei Gao, Ting Huang, Haojun Yuan, Jun Yang, Qinghui Jin, Chunping Jia, Guoxin Mao, Jianlong Zhao
Circulating tumor cells (CTCs) have become an important biomarker for liquid biopsy to monitor tumor progression and indicate response to therapies. Many epithelial cellular adhesion molecule (EpCAM) dependent CTC isolation methods have been developed, which have a limitation for low EpCAM expressed tumor cells. In an effort to overcome these drawbacks, we developed combined immunomagnetic beads (EpCAM, Mucin1 and epidermal growth factor receptor) to sensitively isolate CTCs for immunofluorescence analysis and genetic characterization...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29758927/epithelial-mesenchymal-transition-and-galc-expression-of-circulating-tumor-cells-indicate-metastasis-and-poor-prognosis-in-non-small-cell-lung-cancer
#8
De-Gang Liu, Lei Xue, Jun Li, Qiang Yang, Jiang-Zhou Peng
BACKGROUND: Circulating tumor cells (CTCs) is a promising biomarker for cancer prognosis and monitoring. Molecular characterizing of CTCs could provide beneficial information on the basis of CTCs counting. OBJECTIVE: To investigate the epithelial-mesenchymal transition (EMT) phenotypes and GALC mRNA expression of CTCs in non-small cell lung cancer (NSCLC) patients. METHODS: We analyzed the baseline number, EMT classification, and GALC expression of CTCs in 47 NSCLC patients using CanPatrol platform and RNA in situ hybridization technique...
May 5, 2018: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/29753882/circulating-tumor-microemboli-progress-in-molecular-understanding-and-enrichment-technologies
#9
REVIEW
Muhammad Umer, Ramanathan Vaidyanathan, Nam-Trung Nguyen, Muhammad J A Shiddiky
Circulating tumor cells (CTCs) and their clusters, also known as circulating tumor microemboli (CTM), have emerged as valuable tool that can provide mechanistic insights into the tumor heterogeneity, clonal evolution, and stochastic events within the metastatic cascade. However, recent investigations have hinted that CTM may not be mere aggregates of tumor cells but cells comprising CTM exhibit distinct phenotypic and molecular characteristics in comparison to single CTCs. Moreover, in many cases CTM demonstrated higher metastatic potential and resistance to apoptosis as compared to their single cell counterparts...
May 10, 2018: Biotechnology Advances
https://www.readbyqxmd.com/read/29748004/pd-l1-expression-in-circulating-tumor-cells-of-advanced-non-small-cell-lung-cancer-patients-treated-with-nivolumab
#10
Nicolas Guibert, Myriam Delaunay, Amélie Lusque, Nadia Boubekeur, Isabelle Rouquette, Estelle Clermont, Jean Mourlanette, Sandrine Gouin, Inge Dormoy, Gilles Favre, Julien Mazieres, Anne Pradines
BACKGROUND: Inhibitors of the PD-1/PD-L1 immune checkpoint have become a standard of care in non-small cell lung cancer (NSCLC). Patient selection, currently based on PD-L1 expression on tumor tissue, is limited by its temporal and spatial heterogeneity. We hypothesized that liquid biopsy with PD-L1 analysis on circulating tumor cells (CTCs) might overcome this limitation. METHODS: Blood samples were prospectively collected from patients with advanced NSCLC before nivolumab treatment and at the time of progression...
June 2018: Lung Cancer: Journal of the International Association for the Study of Lung Cancer
https://www.readbyqxmd.com/read/29747380/cancer-diagnosis-using-a-liquid-biopsy-challenges-and-expectations
#11
REVIEW
Francesc Castro-Giner, Sofia Gkountela, Cinzia Donato, Ilaria Alborelli, Luca Quagliata, Charlotte K Y Ng, Salvatore Piscuoglio, Nicola Aceto
The field of cancer diagnostics has recently been impacted by new and exciting developments in the area of liquid biopsy. A liquid biopsy is a minimally invasive alternative to surgical biopsies of solid tissues, typically achieved through the withdrawal of a blood sample or other body fluids, allowing the interrogation of tumor-derived material including circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) fragments that are present at a given time point. In this short review, we discuss a few studies that summarize the state-of-the-art in the liquid biopsy field from a diagnostic perspective, and speculate on current challenges and expectations of implementing liquid biopsy testing for cancer diagnosis and monitoring in the clinical setting...
May 9, 2018: Diagnostics
https://www.readbyqxmd.com/read/29746957/in-vivo-inhibition-of-circulating-tumor-cells-by-two-apoptosis-promoting-circular-aptamers-with-enhanced-specificity
#12
Haiyan Dong, Longyu Han, Jie Wang, Jingjing Xie, Yu Gao, Fangwei Xie, Lee Jia
Circulating tumor cells (CTCs) are known as the root cause of cancer metastasis that accounts for 90% of cancer death. Owing to the rarity of blood CTCs and their microenvironmental complexity, the existing biotechnology could not precisely capture and apoptosize CTCs in vivo for cancer metastasis prevention. Here, we designed two double strand circular aptamers aimed to simultaneously target MUC1 and HER2 surface biomarkers on mesenchymal cancer cells. The circular aptamers are composed of a capture arm for binding and seizing CTCs and a circular body for resisting degradation by exonucleases...
May 7, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29746918/circulating-tumor-cells-in-stage-iv-melanoma-patients
#13
Carolyn S Hall, Merrick Ross, Jessica B Bowman Bauldry, Joshua Upshaw, Mandar G Karhade, Richard Royal, Sapna Patel, Anthony Lucci
BACKGROUND: Management of stage IV melanoma patients remains a challenge. In spite of promising new therapies, many patients develop resistance and progression. The aim of this pilot study was to determine if CTCs are associated with shortened (180-day) progression-free survival (PFS) following a baseline CTC assessment in stage IV melanoma patients. METHODS: A baseline CTC assessment was performed in 93 stage IV melanoma patients using a commercially available immunomagnetic system...
May 7, 2018: Journal of the American College of Surgeons
https://www.readbyqxmd.com/read/29741566/circulating-tumor-cell-increase-as-a-biomarker-of-disease-progression-in-metastatic-castration-resistant-prostate-cancer-patients-with-low-baseline-ctc-counts
#14
D Lorente, D Olmos, J Mateo, D Dolling, D Bianchini, G Seed, P Flohr, M Crespo, I Figueiredo, S Miranda, H I Scher, L W M M Terstappen, J S de Bono
Background: The development of treatment response and surrogate biomarkers for advanced prostate cancer care is an unmet clinical need. Patients with baseline circulating tumor cell counts (BLCTCs)<5/7.5 mL represent a good prognosis subgroup, but are non-evaluable for response assessment (decrease in CTCs). The aim of the study is to determine the value of any increase in CTCs (CTC Progression) as an indicator of progression in prostate cancer patients with low pre-treatment CTCs (<5)...
May 7, 2018: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/29739347/elevated-serum-ras-p21-is-an-independent-prognostic-factor-in-metastatic-breast-cancer
#15
Malgorzata Banys-Paluchowski, Tanja Fehm, Wolfgang Janni, Bahriye Aktas, Peter A Fasching, Sabine Kasimir-Bauer, Karin Milde-Langosch, Klaus Pantel, Brigitte Rack, Sabine Riethdorf, Erich-Franz Solomayer, Isabell Witzel, Volkmar Müller
BACKGROUND: An important component of the RAS signalling pathway, the RAS p21 oncogene, is frequently hyperactivated in breast cancer. Its expression in tumor tissue has been linked to poor clinical outcome. This study was designed to evaluate the clinical relevance of RAS p21 levels in peripheral blood in a large cohort of metastatic breast cancer patients. METHODS: Two hundred fifty-one patients with metastatic breast cancer were enrolled in this prospective, multicentre, open-label, non-randomized study...
May 8, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29733518/downregulation-of-ndr1-contributes-to-metastasis-of-prostate-cancer-cells-via-activating-epithelial-mesenchymal-transition
#16
Juntao Yue, Huimin Sun, Shijie Liu, Fei Yu, Shanshan Wang, Fuli Wang, Ruixiong Shen, Feng Zhu, Lei Zhang, Chen Shao
The 5-year survival rate decreases rapidly once the prostate cancer has invaded distant organs, although patients with localized prostate cancer have a good prognosis. In recent years, increasing numbers of reports showed that circulating tumor cells (CTCs) may play an important role in tumor metastasis and they have stronger potential of invasion and migration compared with their parental cells. In our previous investigation, we isolated CTCs from prostate cancer cell lines PC3. In this study, we found a novel antimetastasis gene NDR1 by analyzing different gene expression between CTCs and PC3...
May 7, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29731846/the-clinical-value-of-detecting-circulating-tumour-cells-in-the-peripheral-blood-of-nasopharyngeal-carcinoma-patients
#17
Zhuo Chen, Lu Xu, Xinhua Xu, Chen Yuan
The aim of the present study as to analyse the associations between circulating tumour cells (CTCs) and the clinical parameters of nasopharyngeal carcinoma (NPC). Peripheral blood (7.5 ml) from 68 first-diagnosed NPC patients was collected to detect and identify CTCs by cluster of differentiation (CD)45 immunomagnetic separation. Immunofluorescent staining of cytokeratin-18, CD45 and DAPI, and fluorescence in situ hybridization were combined with the centromere of chromosome 8 (CEP8) probe method to analyse the associations between CTCs and clinical parameters...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29728125/matrix-stiffness-upregulated-loxl2-promotes-fibronectin-production-mmp9-and-cxcl12-expression-and-bmdcs-recruitment-to-assist-pre-metastatic-niche-formation
#18
Sifan Wu, Qiongdan Zheng, Xiaoxia Xing, Yinying Dong, Yaohui Wang, Yang You, Rongxin Chen, Chao Hu, Jie Chen, Dongmei Gao, Yan Zhao, Zhiming Wang, Tongchun Xue, Zhenggang Ren, Jiefeng Cui
BACKGROUND: Higher matrix stiffness affects biological behavior of tumor cells, regulates tumor-associated gene/miRNA expression and stemness characteristic, and contributes to tumor invasion and metastasis. However, the linkage between higher matrix stiffness and pre-metastatic niche in hepatocellular carcinoma (HCC) is still largely unknown. METHODS: We comparatively analyzed the expressions of LOX family members in HCC cells grown on different stiffness substrates, and speculated that the secreted LOXL2 may mediate the linkage between higher matrix stiffness and pre-metastatic niche...
May 4, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29725990/gene-expression-analysis-of-immunomagnetically-enriched-circulating-tumor-cell-fraction-in-castration-resistant-prostate-cancer
#19
Markéta Škereňová, Veronika Mikulová, Otakar Čapoun, David Švec, Katarína Kološtová, Viktor Soukup, Hana Honová, Tomáš Hanuš, Tomáš Zima
BACKGROUND: Molecular characterization of tumors could be a key to therapeutic decision-making with regards to targeted therapies in castration-resistant prostate cancer (CRPC). A convenient solution may be non-invasive liquid biopsy testing of circulating tumor cells (CTCs). For this reason, CTC-enriched samples obtained by immunomagnetic separation (AdnaTest® ) were studied as a source material for high-throughput gene expression analysis using BioMark™. PATIENTS AND METHODS: CTC-enriched samples from 41 CRPC patients previously determined to be CTC positive using the AdnaTest® were retrospectively re-analysed for androgen receptor (AR) messenger RNA (mRNA), using the updated AdnaTest® ...
May 3, 2018: Molecular Diagnosis & Therapy
https://www.readbyqxmd.com/read/29725451/prognostic-significance-of-circulating-tumor-microemboli-in-patients-with-pancreatic-ductal-adenocarcinoma
#20
Guangdong Wu, Rongrong Zhu, Yatong Li, Yupei Zhao, Menghua Dai
The significance of circulating tumor microemboli (CTMs) in patients with pancreatic ductal adenocarcinoma (PDAC) is still unknown. Thus, the present study used a epithelial cellular adhesion molecule independent subtraction and immunostaining-fluorescence in situ hybridization (SET-iFISH) platform to enumerate circulating tumor cells (CTCs) and CTMs in a total of 86 peripheral blood samples from 19 patients with PDAC. The associations between CTCs and CTMs with clinicopathologic factors and prognoses were analyzed...
May 2018: Oncology Letters
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