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https://www.readbyqxmd.com/read/27931182/exposure-of-bovine-oocytes-and-embryos-to-elevated-non-esterified-fatty-acid-concentrations-integration-of-epigenetic-and-transcriptomic-signatures-in-resultant-blastocysts
#1
K L J Desmet, V Van Hoeck, D Gagné, E Fournier, A Thakur, A M O'Doherty, C P Walsh, M A Sirard, P E J Bols, J L M R Leroy
BACKGROUND: Metabolic stress associated with negative energy balance in high producing dairy cattle and obesity in women is a risk factor for decreased fertility. Non-esterified fatty acids (NEFA) are involved in this pathogenesis as they jeopardize oocyte and embryo development. Growing evidence indicates that maternal metabolic disorders can disturb epigenetic programming, such as DNA methylation, in the offspring. Oocyte maturation and early embryo development coincide with methylation changes and both are sensitive to adverse environments...
December 8, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27927842/parental-contributions-to-early-embryo-development-influences-of-urinary-phthalate-and-phthalate-alternatives-among-couples-undergoing-ivf-treatment
#2
Haotian Wu, Lisa Ashcraft, Brian W Whitcomb, Tayyab Rahil, Ellen Tougias, Cynthia K Sites, J Richard Pilsner
STUDY QUESTION: Are preconception urinary concentrations of phthalates and phthalate alternatives associated with diminished early stage embryo quality in couples undergoing IVF? SUMMARY ANSWER: Male, but not female, urinary concentrations of select metabolites of phthalates and phthalate alternatives are associated with diminished blastocyst quality. WHAT IS KNOWN ALREADY: Although phthalates are endocrine disrupting compounds associated with adverse reproductive health, they are in widespread use across the world...
December 7, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27925340/evaluation-of-epididymis-storage-temperature-and-cryopreservation-conditions-for-improved-mitochondrial-membrane-potential-membrane-integrity-sperm-motility-and-in-vitro-fertilization-in-bovine-epididymal-sperm
#3
M Nichi, T Rijsselaere, Jda Losano, Dsr Angrimani, Gkv Kawai, Igf Goovaerts, A Van Soom, V H Barnabe, Jbp De Clercq, Pej Bols
The maintaining of the epididymis at lower temperatures during storage and transport improves sperm quality. Our study aimed to test whether epididymis storage temperature (post-mortem) and sperm cryopreservation affect sperm kinetics, membrane integrity, mitochondrial potential and fertility capacity. Thirty-six epididymides were collected from 18 bulls after slaughter and divided into two groups: at 4 or 34°C for 2-3 hr. The sperm was collected from the epididymis cauda. The evaluation consisted of computer-assisted sperm analysis (CASA), SYBR14/PI/JC1 to evaluate membrane integrity, mitochondrial membrane potential (MMP) and measurement of lipid peroxidation (TBARS)...
December 7, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27925012/effect-of-nac-on-mouse-gv-oocyte-survival-and-subsequent-embryonic-development-following-vitrfication
#4
S L Yue, Y T Zhang, S W Wang, M Sun, Y C Xing, J Wen, J B Zhou
BACKGROUND: Oocytes that survive cryopreservation may accumulate ROS which are known to bring harmful effects on embryonic development. NAC is an antioxidant which can be a supplement to reduce oxidative stress. However, whether NAC can improve the developmental competence of vitrified GV-oocytes remains unclear. OBJECTIVE: The study was to investigate the effect of NAC on subsequent embryonic developmental competence of mice vitrified GV-oocytes. MATERIALS AND METHODS: This study compared the effects of different concentration of NAC on the cleavage and blastocyst rates of mice vitrified GV-oocytes...
July 2016: Cryo Letters
https://www.readbyqxmd.com/read/27924832/impaired-receptivity-and-decidualization-in-dhea-induced-pcos-mice
#5
Shu-Yun Li, Zhuo Song, Min-Jie Song, Jia-Wen Qin, Meng-Long Zhao, Zeng-Ming Yang
Polycystic ovary syndrome (PCOS), a complex endocrine disorder, is a leading cause of female infertility. An obvious reason for infertility in PCOS women is anovulation. However, success rate with high quality embryos selected by assisted reproduction techniques in PCOS patients still remain low with a high rate of early clinical pregnancy loss, suggesting a problem in uterine receptivity. Using a dehydroepiandrosterone-induced mouse model of PCOS, some potential causes of decreased fertility in PCOS patients were explored...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27924572/isolation-of-mouse-embryonic-stem-cell-lines-in-the-study-of-erk1-2-map-kinase-signaling
#6
Marc K Saba-El-Leil, Christophe Frémin, Sylvain Meloche
Mouse embryonic stem (ES) cells have proven to be invaluable research tools for dissecting the role of signaling pathways in embryonic development, adult physiology, and various diseases. ES cells are amenable to genetic manipulation by classical gene targeting via homologous recombination or by genome editing technologies. These cells can be used to generate genetically modified mouse models or to study the signaling circuitry regulating self-renewal and early lineage commitment. In this chapter, we describe methods used for the isolation and establishment of mouse ES cell lines from blastocyst embryos and for the measurement of ERK1/2 activity in ES cells...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924510/global-protein-expression-analysis-of-molecular-markers-of-ds-1-47-a-component-of-implantation-promoting-traditional-chinese-medicine
#7
Yan-Ling Li, Xiao-Yan Zhang, Yu Leng, Yan-Li Wu, Jing Li, Yun-Xia Wu
This study investigated the molecular markers of DS-1-47, a component of an implantation- promoting traditional Chinese medicine consisting of Astragalus mongholicus, Atractylodes macrocephala, Scutellaria baicalensis and Dipsacales, in an attempt to clarify the molecular mechanism and action targets of DS-1-47. Controlled ovarian stimulation (COS) method was used to establish the implantation dysfunction models of mice. Animals were divided into normal pregnant group, COS model group and DS-1-47 group. Laser capture microdissection-double dimensional electrophoresis-mass spectrum (LCM-DE-MS) was used to analyze the uterine protein molecules that were possibly involved in the promotion of implantation...
December 2016: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/27924460/proteomic-profile-of-maternal-aged-blastocoel-fluid-suggests-a-novel-role-for-ubiquitin-system-in-blastocyst-quality
#8
Gabriella Tedeschi, Elena Albani, Elena Monica Borroni, Valentina Parini, Anna Maria Brucculeri, Elisa Maffioli, Armando Negri, Simona Nonnis, Mauro Maccarrone, Paolo Emanuele Levi-Setti
PURPOSE: The etiology of maternal aging, a common cause of female factor infertility and a rate-limiting step in vitro fertilization (IVF) success, remains still unclear. Proteomic changes responsible for the impaired successful pregnancy outcome after IVF with aged blastocysts have not been yet evaluated. The objective of this prospective study was to employ proteomic techniques and bioinformatic tools to enlight differences at the protein level in blastocoel fluid of aged and younger woman...
December 6, 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27923412/effects-of-vitrification-on-blastomere-viability-and-cytoskeletal-integrity-in-mouse-embryos
#9
Zenon Oikonomou, Katerina Chatzimeletiou, Antonia Sioga, Louisa Oikonomou, Basil C Tarlatzis, Efstratios Kolibianakis
Vitrification is widely used to cryopreserve supernumerary embryos following in vitro fertilization (IVF). The mouse model was used to investigate the effects of vitrification on blastomere viability, using viability markers, and on the cytoskeleton, by analysing spindle/chromosome configurations, using confocal scanning microscopy. Ninety cleavage and morula stage dimethyl sulphoxide (DMSO)/EG vitrified mouse embryos were either processed immediately following warming for viability assessment by labelling with the fluorescent markers carboxyfluorescein-diacetate succinimidylester (CFSE) and propidium iodide (PI) or were cultured to the blastocyst stage and immunostained with α-tubulin antibody to visualize microtubules and DAPI or PI to visualize DNA...
December 7, 2016: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/27921089/effect-of-l-carnitine-on-in-vitro-developmental-rate-the-zona-pellucida-and-hatching-of-blastocysts-and-their-cell-numbers-in-mouse-embryos
#10
Nasrin Khanmohammadi, Mansoureh Movahedin, Manouchehr Safari, Hamid Reza Sameni, Behpour Yousefi, Behnaz Jafari, Sam Zarbakhsh
: L-carnitine (LC) is an antioxidant with the ability to promote the growth in vitro embryo. OBJECTIVE: The goal was to evaluate the effect of LC on some indicators of embryo development and blastocyst quality including zona pellucid (ZP) thickness, the hatching of blastocysts and their cell numbers. MATERIALS AND METHODS: Mouse embryos were randomly divided into five groups and incubated with different concentrations of LC (I; 0, II; 0.5, III; 1, IV; 2 and V; 4 mg/ml) from 2-cell to hatched blastocyst...
October 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/27921085/effects-of-autologous-platelet-rich-plasma-on-implantation-and-pregnancy-in-repeated-implantation-failure-a-pilot-study
#11
Leila Nazari, Saghar Salehpour, Sedighe Hoseini, Shahrzad Zadehmodarres, Ladan Ajori
BACKGROUND: Repeated implantation failure (RIF) is a major challenge in reproductive medicine and despite several methods that have been described for management, there is little consensus on the most effective one. OBJECTIVE: This study was conducted to evaluate the effectiveness of platelet-rich plasma in improvement of pregnancy rate in RIF patients. MATERIALS AND METHODS: Twenty women with a history of RIF who were candidates for frozen-thawed embryo transfer were recruited in this study...
October 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/27918728/cholesterol-metabolism-in-rabbit-blastocysts-under-maternal-diabetes
#12
S Mareike Pendzialek, Maria Schindler, Torsten Plösch, Jacqueline Gürke, Elisa Haucke, Stefanie Hecht, Bernd Fischer, Anne Navarrete Santos
In the rabbit reproductive model, maternal experimentally induced insulin-dependent diabetes mellitus (expIDD) leads to accumulation of lipid droplets in blastocysts. Cholesterol metabolism is a likely candidate to explain such metabolic changes. Therefore, in the present study we analysed maternal and embryonic cholesterol concentrations and expression of related genes in vivo (diabetic model) and in vitro (embryo culture in hyperglycaemic medium). In pregnant expIDD rabbits, the serum composition of lipoprotein subfractions was changed, with a decrease in high-density lipoprotein cholesterol and an increase in very low-density lipoprotein cholesterol; in uterine fluid, total cholesterol concentrations were elevated...
December 6, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27918161/-current-options-of-preimplantion-genetic-screening-and-preimplantation-genetic-diagnostics
#13
V Šimečková
OBJECTIVE: The aim of this work is to summarize the current knowledge about preimplantation genetic screening and diagnostics. DESIGN: A review article. SETTING: Department of Gynecology and Obstetrics, District Hospital Šternberk, IVF Clinic, Olomouc. RESULTS: Preimplantation genetic testing is a complex of genetic and molecular cytogenetic examinations, which can help to detect abnormalities in embryos before transfer into the uterus of the mother...
2016: Ceská Gynekologie
https://www.readbyqxmd.com/read/27917907/ecat1-is-essential-for-human-oocyte-maturation-and-pre-implantation-development-of-the-resulting-embryos
#14
Changyu Liu, Min Li, Tianjie Li, Hongcui Zhao, Jin Huang, Yun Wang, Qian Gao, Yang Yu, Qinghua Shi
ECAT1 is a subunit of the subcortical maternal complex that is required for cell cycle progression during pre-implantation embryonic development; however, its exact function remains to be elucidated. Here we investigated the expression of ECAT1 in human ovarian tissue, oocytes and pre-implantation embryos and assessed its function by using RNA interference (RNAi) in oocytes. ECAT1 mRNA was highly expressed in human oocytes and zygotes, as well as in two-cell, four-cell and eight-cell embryos, but declined significantly in morulae and blastocysts...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27916207/integrating-insulin-into-single-step-culture-medium-regulates-human-embryo-development-in%C3%A2-vitro
#15
Mohamed Fawzy, Mohamed Sabry, Mohamed Nour, Mohamed Y Abdelrahman, Eman Roshdy, Yasmin Magdi, Hazem Abdelghafar
OBJECTIVE: To evaluate the effect of supplementing single-step embryo culture medium with insulin on human embryo development. DESIGN: Comparative study. SETTING: Two private centers. PATIENT(S): The study involved a sibling oocyte split of 5,142 retrieved oocytes from 360 patients. INTERVENTION(S): Sibling oocytes split after intracytoplasmic sperm injection for culture from day 0 through day 5 or 6 in insulin-supplemented or control medium...
December 1, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27913778/effect-of-deslorelin-acetate-treatment-in-oocyte-recovery-and-in-vitro-embryo-production-in-domestic-cats
#16
Camila Louise Ackermann, Eduardo Trevisol, Leticia Ferrari Crocomo, Tatiana da Silva Rascado, Rodrigo Volpato, Carlos Renato de Freitas Guaitolini, Carlize Lopes, Talita de Almeida Costa, Maria Denise Lopes
OBJECTIVES: The present study investigated the effect of contraceptive treatment with deslorelin acetate on in vitro embryo production and oocyte recovery in domestic queens. METHODS: Twenty-one mature domestic cats were used. Eleven queens (treated group) and one tom were kept in an experimental cattery, and 10 queens were privately owned (control group). When in interestrus or diestrus (day 0) a deslorelin acetate implant (Suprelorin, 4.7 mg/animal) was inserted into the subcutaneous tissue of the interscapular region in all queens in the treated group...
December 2, 2016: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/27913136/cytogenetic-analysis-of-the-retained-products-of-conception-after-missed-abortion-following-blastocyst-transfer-a-retrospective-large-scale-single-centre-study
#17
Tomoya Segawa, Tomoko Kuroda, Keiichi Kato, Masako Kuroda, Kenji Omi, Osamu Miyauchi, Yoshiaki Watanabe, Tsuyoshi Okubo, Hisao Osada, Shokichi Teramoto
Cytogenetic analysis of the retained products of conception (POC) is the most effective test for identifying miscarriage causes. However, there has been no large-scale study limited to blastocyst transfer. This study retrospectively reports the findings of 1030 cases in which POC analysis was performed after missed abortion following single blastocyst transfer performed at the Shinbashi Yume Clinic. We identified 19.4% as normal karyotypes and 80.6% as aneuploid. These cases broke down into: 62.3% trisomy; 7...
November 21, 2016: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/27912901/individualized-versus-conventional-ovarian-stimulation-for-in%C3%A2-vitro-fertilization-a-multicenter-randomized-controlled-assessor-blinded-phase-3-noninferiority-trial
#18
Anders Nyboe Andersen, Scott M Nelson, Bart C J M Fauser, Juan Antonio García-Velasco, Bjarke M Klein, Joan-Carles Arce
OBJECTIVE: To compare the efficacy and safety of follitropin delta, a new human recombinant FSH with individualized dosing based on serum antimüllerian hormone (AMH) and body weight, with conventional follitropin alfa dosing for ovarian stimulation in women undergoing IVF. DESIGN: Randomized, multicenter, assessor-blinded, noninferiority trial (ESTHER-1). SETTING: Reproductive medicine clinics. PATIENT(S): A total of 1,329 women (aged 18-40 years)...
November 22, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27911818/planar-cell-polarity-signaling-in-the-uterus-directs-appropriate-positioning-of-the-crypt-for-embryo-implantation
#19
Jia Yuan, Jeeyeon Cha, Wenbo Deng, Amanda Bartos, Xiaofei Sun, Hsin-Yi Henry Ho, Jean-Paul Borg, Terry P Yamaguchi, Yingzi Yang, Sudhansu K Dey
Blastocyst implantation is a complex process requiring coordination of a dynamic sequence of embryo-uterine interactions. Blood vessels enter the uterus from the mesometrium, demarcating the uterus into mesometrial (M) and antimesometrial (AM) domains. Implantation occurs along the uterine longitudinal axis within specialized implantation chambers (crypts) that originate within the evaginations directed from the primary lumen toward the AM domain. The morphological orientation of crypts in rodent uteri was recognized more than a century ago, but the mechanism remained unknown...
November 28, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27911036/properties-of-embryoid-bodies
#20
REVIEW
Joshua M Brickman, Palle Serup
Embryoid bodies (EBs) have been popular in vitro differentiation models for pluripotent stem cells for more than five decades. Initially, defined as aggregates formed by embryonal carcinoma cells, EBs gained more prominence after the derivation of karyotypically normal embryonic stem cells from early mouse blastocysts. In many cases, formation of EBs constitutes an important initial step in directed differentiation protocols aimed at generated specific cell types from undifferentiated stem cells. Indeed state-of-the-art protocols for directed differentiation of cardiomyocytes still rely on this initial EB step...
December 2, 2016: Wiley Interdisciplinary Reviews. Developmental Biology
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