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https://www.readbyqxmd.com/read/29773834/embryonic-pou5f1-is-required-for-expanded-bovine-blastocyst-formation
#1
Bradford W Daigneault, Sandeep Rajput, George W Smith, Pablo J Ross
POU5F1 is a transcription factor and master regulator of cell pluripotency with indispensable roles in early embryo development and cell lineage specification. The role of embryonic POU5F1 in blastocyst formation and cell lineage specification differs between mammalian species but remains completely unknown in cattle. The CRISPR/Cas9 system was utilized for targeted disruption of the POU5F1 gene by direct injection into zygotes. Disruption of the bovine POU5F1 locus prevented blastocyst formation and was associated with embryonic arrest at the morula stage...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29772707/pimt-ncoa6ip-deletion-in-the-mouse-heart-causes-delayed-cardiomyopathy-attributable-to-perturbation-in-energy-metabolism
#2
Yuzhi Jia, Ning Liu, Navin Viswakarma, Ruya Sun, Mathew J Schipma, Meng Shang, Edward B Thorp, Yashpal S Kanwar, Bayar Thimmapaya, Janardan K Reddy
PIMT/NCOA6IP, a transcriptional coactivator PRIP/NCOA6 binding protein, enhances nuclear receptor transcriptional activity. Germline disruption of PIMT results in early embryonic lethality due to impairment of development around blastocyst and uterine implantation stages. We now generated mice with Cre-mediated cardiac-specific deletion of PIMT (csPIMT-/- ) in adult mice. These mice manifest enlargement of heart, with nearly 100% mortality by 7.5 months of age due to dilated cardiomyopathy. Significant reductions in the expression of genes (i) pertaining to mitochondrial respiratory chain complexes I to IV; (ii) calcium cycling cardiac muscle contraction ( Atp2a1 , Atp2a2 , Ryr2 ); and (iii) nuclear receptor PPAR- regulated genes involved in glucose and fatty acid energy metabolism were found in csPIMT-/- mouse heart...
May 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29770989/vitrification-for-bovine-embryos-with-low-quality-grade
#3
Hiroshi Tajimi, Tsugumi Yamazaki, Syoko Oike, Tatsuyuki Yoshida, Konosuke Okada, Masashige Kuwayama, Hitoshi Ushijima
This study was conducted to examine the utility of vitrification for bovine embryos with low-quality grade, and simple cryoprotectants dilution method for practitioners. In Experiment 1, survival of frozen embryos was compared with that of vitrified embryos using minimum volume cooling (MVC). Then, vitrified embryos were used to confirm the optimum sucrose concentration in Experiment 2. The survival rates of embryos that had been vitrified following diluted cryoprotectants with the one-step in-straw method were compared with those of fresh control embryos in Experiment 3...
May 17, 2018: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/29770509/molecular-characteristics-of-granulosa-and-cumulus-cells-and-oocyte-competence-in-nelore-cows-with-low-and-high-numbers-of-antral-follicles
#4
C O Rosa, Lsr Marinho, Pra da Rosa, M P De Cesaro, P A Lunardelli, K C Silva-Santos, A C Basso, V Bordignon, M M Seneda
The aims of this study were to investigate whether the number of antral follicles (AF) in the ovaries of Nelore cows is influenced with the developmental competence of oocytes to reach the blastocyst stage and to quantify the mRNA abundance of genes associated with folliculogenesis and oogenesis in granulosa and cumulus cells. A total of 168 cows were distributed into two experimental groups according to the number of AF, low (≤31) and high AF (≥92), which were determined based on the mean number of AF (61...
May 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29769451/effect-of-ferulic-acid-supplementation-on-the-developmental-competence-of-porcine-embryos-during-in-vitro-maturation
#5
Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Takayuki Hirano, Toshiki Kunihara, Takeshige Otoi
The value of laboratory and genetically-modified pigs is becoming increasingly clear; however, their in vitro development remains inefficient. Trans-ferulic acid (trans-FA) is an aromatic compound that is abundant in plant cell walls, and which exhibits antioxidant effects in vitro. Trans-FA is known to improve sperm viability and motility; however, its effects on porcine oocytes are unknown. Our aim was to investigate the effects of trans-FA supplementation during in vitro maturation on the meiotic and developmental competence of porcine oocytes...
May 16, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29766333/treatment-of-donor-cells-with-recombinant-kdm4d-protein-improves-preimplantation-development-of-cloned-ovine-embryos
#6
Yumei Zhang, Qianqian Wang, Kailing Liu, Enen Gao, Hong Guan, Jian Hou
Incomplete epigenetic reprogramming is one of the major factors affecting the development of embryos cloned by somatic cell nuclear transfer (SCNT). Histone 3 lysine 9 (H3K9) trimethylation has been identified as a key barrier to efficient reprogramming by SCNT. The aim of this study was to explore a method of downregulating H3K9me3 levels in donor cells by using histone lysine demethylase (KDM) protein. When sheep fetal fibroblast cells were treated with recombinant human KDM4D protein (rhKDM4D), the levels of H3K9 trimethylation and dimethylation were both significantly decreased...
May 15, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29765076/resveratrol-significantly-improves-the-fertilisation-capacity-of-bovine-sex-sorted-semen-by-inhibiting-apoptosis-and-lipid-peroxidation
#7
Chong-Yang Li, Ya-Han Zhao, Hai-Sheng Hao, Hao-Yu Wang, Jin-Ming Huang, Chang-Liang Yan, Wei-Hua Du, Yun-Wei Pang, Pei-Pei Zhang, Yan Liu, Hua-Bin Zhu, Xue-Ming Zhao
The aim of this study was to test the effects of five different concentrations (0, 10-3 , 10-4 , 10-5 , and 10-6  M) of resveratrol (Res) supplementation in bull sperm washing and fertilisation medium on levels of reactive oxygen species (ROS), phosphatidylserine (PS) externalisation, mitochondrial membrane potential (Δψm), ATP and malondialdehyde (MDA), acrosomal integrity, blastocyst rate, and blastocyst quality after in vitro fertilisation (IVF). The results for sex-sorted sperm from three bulls showed: (1) ROS and MDA levels in 10-3  M and 10-4  M Res groups were significantly lower than those of controls (P < 0...
May 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29764212/the-clinical-application-of-single-sperm-based-snp-haplotyping-for-pgd-of-osteogenesis-imperfecta
#8
Linjun Chen, Zhenyu Diao, Zhipeng Xu, Jianjun Zhou, Guijun Yan, Haixiang Sun
Osteogenesis imperfecta (OI) is a genetically heterogeneous disorder, presenting either autosomal dominant, autosomal recessive or X-linked inheritance patterns. The majority of OI cases are autosomal dominant and are caused by heterozygous mutations in either the COL1A1 or COL1A2 gene. In these dominant disorders, allele dropout (ADO) can lead to misdiagnosis in preimplantation genetic diagnosis (PGD). Polymorphic markers linked to the mutated genes have been used to establish haplotypes for identifying ADO and ensuring the accuracy of PGD...
May 15, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29762687/glycine-treatment-enhances-developmental-potential-of-porcine-oocytes-and-early-embryos-by-inhibiting-apoptosis
#9
Suo Li, Qing Guo, Yu-Meng Wang, Zi-Yue Li, Jin-Dan Kang, Xi-Jun Yin, Xin Zheng
Glycine, a component of glutathione (GSH), plays an important role in protection from reactive oxygen species (ROS) and inhibition of apoptosis. The aim of this study was to determine the effect of glycine on in vitro maturation (IVM) of porcine oocytes and their developmental competence after parthenogenetic activation (PA). We examined nuclear maturation, ROS levels, apoptosis, mitochondrial membrane potential (ΔΨm), and ATP concentration, as well as the expression of several genes related to oocyte maturation and development...
May 12, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29761655/fennel-induces-cytotoxic-effects-against-testicular-germ-cells-in-mice-evidences-for-suppressed-pre-implantation-embryo-development
#10
Aram Minas, Gholamreza Najafi, Ali Shalizar Jalali, Mazdak Razi
Foeniculum vulgare (FVE; fennel) is an aromatic plant belonging to Umbelliferae family, which is widely used in traditional societies because of its different pharmaceutical properties. To uncover the fennel-derived essential oil (FVEO)-induced effects on male reproductive potential, 24 mature male albino mice were divided into, control, 0.37, 0.75, and 1.5 mg kg-1 FVEO-received groups. Following 35 days, the animals were euthanized and the testicular tissue and sperm samples were collected. The histological alterations, tubular differentiation (TDI), spermiogenesis (SPI) indices, apoptosis ratio, and RNA damage of germinal cells were analyzed...
May 15, 2018: Environmental Toxicology
https://www.readbyqxmd.com/read/29758560/prospective-randomized-trial-comparing-embryo-transfers-of-cases-with-and-without-catheter-rotation-during-its-withdrawal
#11
Cigdem Yayla Abide, Enis Ozkaya, Ilhan Sanverdi, Evrim Bostancı Ergen, Meryem Kurek Eken, Belgin Devranoglu, Bulent Emre Bilgiç, Cetin Kilicci, Semra Kayatas Eser
OBJECTIVE: To compare embryo transfer (ET) technique based on catheter rotation during its withdrawal in cases with unexplained infertility in a prospective, randomized trial (NCT03097042). METHODS: Two hundred intracytoplasmic sperm injection (ICSI) patients undergoing ET with cleaving or blastocyst-stage fresh embryos were randomized into 2 groups: cases with (n = 100), and without (n = 100) catheter rotation during its withdrawal. Groups were matched for age and some clinical parameters...
May 14, 2018: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/29757275/isolation-of-primary-human-decidual-cells-from-the-fetal-membranes-of-term-placentae
#12
Tali Farine, Michael Parsons, Stephen Lye, Oksana Shynlova
The decidua, also known as the pregnant endometrium, is a critically important reproductive tissue. Decidual cells, comprised mainly of decidualized stromal cells and immune cells, are responsible for the secretion of hormonal and inflammatory factors which are critical for successful blastocyst implantation, placental development and play a role in the initiation of labor at term and preterm. Many pregnancy complications can arise from perturbations of a fine balance of different cell populations comprising decidua...
April 30, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29751777/high-efficiency-derivation-of-human-embryonic-stem-cell-lines-using-a-culture-system-with-minimized-trophoblast-cell-proliferation
#13
Chuti Laowtammathron, Pimjai Chingsuwanrote, Roungsin Choavaratana, Suphadtra Phornwilardsiri, Ketsara Sitthirit, Chidchanok Kaewjunun, Orawan Makemaharn, Papussorn Terbto, Supaporn Waeteekul, Chanchao Lorthongpanich, Yaowalak U-Pratya, Pimonwan Srisook, Pakpoom Kheolamai, Surapol Issaragrisil
BACKGROUND: Due to their extensive self-renewal and multilineage differentiation capacity, human embryonic stem cells (hESCs) have great potential for studying developmental biology, disease modeling, and developing cell replacement therapy. The first hESC line was generated in 1998 by culturing inner cell mass (ICM) cells isolated from human blastocysts using an immunosurgery technique. Since then, many techniques including mechanical ICM isolation, laser dissection, and whole embryo culture have been used to derive hESC lines...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29748644/live-cell-imaging-of-nuclear-chromosomal-dynamics-in-bovine-in-vitro-fertilised-embryos
#14
Tatsuma Yao, Rie Suzuki, Natsuki Furuta, Yuka Suzuki, Kyoko Kabe, Mikiko Tokoro, Atsushi Sugawara, Akira Yajima, Tomohiro Nagasawa, Satoko Matoba, Kazuo Yamagata, Satoshi Sugimura
Nuclear/chromosomal integrity is an important prerequisite for the assessment of embryo quality in artificial reproductive technology. However, lipid-rich dark cytoplasm in bovine embryos prevents its observation by visible light microscopy. We performed live-cell imaging using confocal laser microscopy that allowed long-term imaging of nuclear/chromosomal dynamics in bovine in vitro fertilised (IVF) embryos. We analysed the relationship between nuclear/chromosomal aberrations and in vitro embryonic development and morphological blastocyst quality...
May 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29748642/gestational-oral-low-dose-estradiol-17%C3%AE-induces-altered-dna-methylation-of-cdkn2d-and-psat1-in-embryos-and-adult-offspring
#15
Vera A van der Weijden, Veronika L Flöter, Susanne E Ulbrich
Endocrine disrupting chemicals (EDC) interfere with the natural hormone balance and may induce epigenetic changes through exposure during sensitive periods of development. In this study, the effects of short-term estradiol-17β (E2) exposure on various tissues of pregnant sows (F0 ) and on day 10 blastocysts (F1 ) were assessed. Intergenerational effects were investigated in the liver of 1-year old female offspring (F1 ). During gestation, sows were orally exposed to two low doses and a high dose of E2 (0.05, 10, and 1000 µg/kg body weight/day)...
May 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29746572/evaluation-of-a-novel-non-invasive-preimplantation-genetic-screening-approach
#16
Valeriy Kuznyetsov, Svetlana Madjunkova, Ran Antes, Rina Abramov, Gelareh Motamedi, Zenon Ibarrientos, Clifford Librach
OBJECTIVE: To assess whether embryonic DNA isolated from blastocyst culture conditioned medium (BCCM) combined with blastocoel fluid (BF) could be used for blastocyst stage non-invasive preimplantation genetic testing for chromosomal aneuploidy (non-invasive preimplantation genetic screening, NIPGS). PATIENTS: 47 embryos from 35 patients undergoing IVF. INTERVENTIONS: DNA analysis of combined BCCM plus BF in comparison with trophectoderm (TE) biopsy and/or whole blastocyst (WB)using next generation sequencing (NGS)...
2018: PloS One
https://www.readbyqxmd.com/read/29745895/g9a-regulates-temporal-preimplantation-developmental-program-and-lineage-segregation-in-blastocyst
#17
Jan J Zylicz, Maud Borensztein, Frederick Ck Wong, Yun Huang, Caroline Lee, Sabine Dietmann, M Azim Surani
Early mouse development is regulated and accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2). Previously, we provided insights into its role in post-implantation development (Zylicz et al., 2015). Here we explore the impact of depleting the maternally inherited G9a in oocytes on development shortly after fertilisation. We show that G9a accumulates typically at 4 to 8-cell stage to promote timely repression of a subset of 4-cell stage-specific genes...
May 10, 2018: ELife
https://www.readbyqxmd.com/read/29745447/dna-methylation-pattern-of-bovine-blastocysts-associated-to-hyperinsulinemia-in-vitro
#18
Denise Laskowski, Patrice Humblot, Marc-André Sirard, Ylva Sjunnesson, Naveed Jhamat, Renée Båge, Göran Andersson
Insulin functions as a regulator of metabolism and plays an important role in reproduction. Hyperinsulinemia is often observed in obesity and diabetes type 2 and is known to impair fertility, but the underlying molecular mechanisms are only partly understood. Metabolic programming through epigenetic mechanisms such as DNA methylation during embryonic development can lead to health implications for the offspring later in life. Our aim was to study the potential effect of hyperinsulinemia on gene expression and DNA methylation of embryos by adding insulin (0...
May 10, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29740477/determination-of-oocyte-manipulation-zygote-manipulation-and-genome-reprogramming-effects-on-the-transcriptomes-of-bovine-blastocysts
#19
Byungkuk Min, Jung S Park, Yong-Kook Kang
Somatic cell nuclear transfer (scNT) embryos suffer from damage caused by micro-operation (manipulation) and inefficient genome reprograming that hinder their normal development at different levels and in distinct ways. These two effects are inseparable in the nature of the scNT embryo, although methods to separately measure them are needed to improve scNT technology and evaluate incoming reprogramming tools. As an attempt to meet these demands, we made bovine sham nuclear-transfer (shNT) blastocysts, special embryos made with a standard nuclear-transfer procedure at the zygote stage, while retaining an intact genome...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29740154/additional-mitochondrial-dna-influences-the-interactions-between-the-nuclear-and-mitochondrial-genomes-in-a-bovine-embryo-model-of-nuclear-transfer
#20
Kanokwan Srirattana, Justin C St John
We generated cattle embryos using mitochondrial supplementation and somatic cell nuclear transfer (SCNT), named miNT, to determine how additional mitochondrial DNA (mtDNA) modulates the nuclear genome. To eliminate any confounding effects from somatic cell mtDNA in intraspecies SCNT, donor cell mtDNA was depleted prior to embryo production. Additional oocyte mtDNA did not affect embryo development rates but increased mtDNA copy number in blastocyst stage embryos. Moreover, miNT-derived blastocysts had different gene expression profiles when compared with SCNT-derived blastocysts...
May 8, 2018: Scientific Reports
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