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https://www.readbyqxmd.com/read/28211357/preimplantation-genetic-diagnosis-and-screening-by-array-comparative-genomic-hybridisation-experience-of-more-than-100-cases-in-a-single-centre
#1
J Fc Chow, W Sb Yeung, V Cy Lee, E Yl Lau, P C Ho, E Hy Ng
INTRODUCTION: Preimplantation genetic screening has been proposed to improve the in-vitro fertilisation outcome by screening for aneuploid embryos or blastocysts. This study aimed to report the outcome of 133 cycles of preimplantation genetic diagnosis and screening by array comparative genomic hybridisation. METHODS: This study of case series was conducted in a tertiary assisted reproductive centre in Hong Kong. Patients who underwent preimplantation genetic diagnosis for chromosomal abnormalities or preimplantation genetic screening between 1 April 2012 and 30 June 2015 were included...
February 17, 2017: Hong Kong Medical Journal, Xianggang Yi Xue za Zhi
https://www.readbyqxmd.com/read/28209499/blastocyst-development-after-intracytoplasmic-sperm-injection-of-equine-oocytes-vitrified-at-the-germinal-vesicle-stage
#2
Heloísa Siqueira Canesin, Joao Gatto Brom-de-Luna, Young-Ho Choi, Isabel Ortiz, Mouhamadou Diaw, Katrin Hinrichs
We evaluated the meiotic and developmental competence of GV-stage equine oocytes vitrified under different conditions. In a preliminary study, using dimethyl sulfoxide (D), ethylene glycol (EG) and sucrose (S) as cryoprotectants, the maturation rate was higher for cumulus-oocyte complexes (COCs) held overnight before vitrification (37%) than for those vitrified immediately (14%; P < 0.05). Thereafter, all COCs were held overnight before vitrification. In Experiment 1 we compared 1 min (1 m) and 4 min (4 m) exposure to vitrification and warming solutions; oocytes that subsequently matured were fertilized by ICSI...
February 13, 2017: Cryobiology
https://www.readbyqxmd.com/read/28203704/blocking-connexin-channels-improves-embryo-development-of-vitrified-bovine-blastocysts%C3%A2
#3
Nerea Ortiz-Escribano, Katarzyna Joanna Szymańska, Melissa Bol, Lynn Vandenberghe, Elke Decrock, Mario Van Poucke, Luc Peelman, Etienne Van den Abbeel, Ann Van Soom, Luc Leybaert
No abstract text is available yet for this article.
February 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28202179/effects-of-melatonin-in-rats-in-the-initial-third-stage-of-pregnancy-exposed-to-sub-lethal-doses-of-herbicides
#4
Lécio Leone de Almeida, Álvaro Aguiar Coelho Teixeira, Anísio Francisco Soares, Franklin Magliano da Cunha, Valdemiro Amaro da Silva, Leucio Duarte Vieira Filho, Valéria Wanderley-Teixeira
: Exposure to the herbicides Paraquat (PQ) and Roundup(®) may cause cell lesions due to an increase in oxidative stress levels in different biological systems, even in the reproductive system. OBJECTIVE: Evaluate the possible changes in reproductive parameters and hepatic, as well as its prevention by simultaneous application of melatonin. METHODS: Thirty-five female rats at the age of 3 months were divided into seven groups: three groups exposed to sub-lethal doses of the herbicides PQ (50mg/kg) and Roundup(®) (500mg/kg) (n=5, G2, G3 and G4); three groups exposed to herbicides and simultaneous treatment with 10mg/kg of Melatonin (n=5, G5, G6 and G7) and control group (n=5, G1) from the first to the seventh day of pregnancy...
February 12, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28196411/effects-of-culture-dimensions-on-maintenance-of-porcine-inner-cell-mass-derived-cell-self-renewal
#5
Song Baek, Na Rae Han, Jung Im Yun, Jae Yeon Hwang, Minseok Kim, Choon Keun Park, Eunsong Lee, Seung Tae Lee
Despite the fact that porcine embryonic stem cells (ESCs) are a practical study tool, in vitro long-term maintenance of these cells is difficult in a two-dimensional (2D) microenvironment using cellular niche or extracellular matrix proteins. However, a three-dimensional (3D) microenvironment, similar to that enclosing the inner cell mass of the blastocyst, may improve in vitro maintenance of self-renewal. Accordingly, as a first step toward constructing a 3D microenvironment optimized to maintain porcine ESC self-renewal, we investigated different culture dimensions for porcine ICM-derived cells to enhance the maintenance of self-renewal...
February 15, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28193316/polydatin-improves-the-developmental-competence-of-bovine-embryos-in-vitro-via-induction-of-sirtuin-1-sirt1
#6
Imran Khan, Sung Woo Kim, Kyung-Lim Lee, Seok-Hwan Song, Ayman Mesalam, M M R Chowdhury, Zia Uddin, Ki Hun Park, Il-Keun Kong
The aim of the present study was to investigate the beneficial effect of polydatin (PD), the glycoside form of resveratrol, on embryo development in vitro. Oocytes were aspirated from ovaries of Korean Hanwoo cows and cultured until Day 8 in a humidified atmosphere of 5% CO2 in air at 38.5°C. Protein and gene expression levels were determined through confocal microscopy and reverse transcription-polymerase chain reaction respectively, whereas the number of total and apoptotic cells in Day 8 blastocysts was determined using Hoechst 33342 staining and terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling...
February 14, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28191700/supplement-of-autologous-ooplasm-into-porcine-somatic-cell-nuclear-transfer-embryos-does-not-alter-embryo-development
#7
W-J Lee, J-H Lee, R-H Jeon, S-J Jang, S-C Lee, J-S Park, S-L Lee, W-A King, G-J Rho
Somatic cell nuclear transfer (SCNT) is considered as the technique in which a somatic cell is introduced into an enucleated oocyte to make a cloned animal. However, it is unavoidable to lose a small amount of the ooplasm during enucleation step during SCNT procedure. The present study was aimed to uncover whether the supplement of autologous ooplasm could ameliorate the oocyte competence so as to improve low efficiency of embryo development in porcine SCNT. Autologous ooplasm-transferred (AOT) embryos were generated by the supplementation with autologous ooplasm into SCNT embryos...
February 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28188961/high-serum-progesterone-associated-with-infertility-in-a-woman-with-nonclassic-congenital-adrenal-hyperplasia
#8
Yoshimasa Kawarai, Hiroshi Ishikawa, Tomoya Segawa, Shokichi Teramoto, Tomoaki Tanaka, Makio Shozu
Nonclassic congenital adrenal hyperplasia (NCAH) is an autosomal-recessive disorder caused by 21-hydroxylase deficiency and manifests as hirsutism and oligomenorrhea due to excess adrenal androgen and progesterone. We report a case of a woman with NCAH who showed continuous high serum progesterone levels in the follicular phase associated with impaired folliculogenesis. NCAH was diagnosed based on high 17-hydroxyprogesterone levels after rapid adrenocorticotropic hormone loading, and three heterozygous missense mutations in CYP21A2, encoding 21-hydroxylase, were identified...
February 11, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28188593/re-analysis-of-aneuploidy-blastocysts-with-an-inner-cell-mass-and-different-regional-trophectoderm-cells
#9
Jin Huang, Liying Yan, Sijia Lu, Nan Zhao, Jie Qiao
PURPOSE: The purpose of this study is to explore which part of the trophectoderm best represents the inner cell mass after aCGH analysis. METHODS: Fifty-one preimplantation genetic diagnosis/preimplantation genetic screening of abnormal blastocysts diagnosed by array comparative genomic hybridization were included in this study. Blastocysts were thawed, incubated for 3 to 4 h, and then biopsied. Four regions were biopsied per blastocyst, including the inner cell mass (ICM), trophectoderm (TE) cells opposite the ICM, TE cells at the upper right of the ICM, and TE cells at the lower right of the ICM...
February 10, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28186636/triplet-pregnancy-from-the-transfer-of-two-blastocysts-demonstrating-a-twin-reversed-arterial-perfusion-sequence-with-a-conjoined-twins-pump-fetus
#10
Hongxia Yuan, Qichang Zhou, Jing Li, Shi Zeng
The incidence of monochorionic gestation is increased eight-fold following assisted reproductive techniques [1]. Monochorionic triplet pregnancies are rare, occurring in 1 in 45 500 deliveries [2], and are associated with high mortality and morbidity. Both conjoined twins and the twin reversed arterial perfusion (TRAP) sequence are unique complications of monochorionic pregnancy. The present study describes transvaginal ultrasonography findings from a monochorionic diamniotic triplet pregnancy at 10 weeks of gestation following double embryo transfer; a TRAP sequence, with a pair of conjoined twins as a "pump" fetus was identified...
February 10, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28186371/gene-expression-and-epigenetic-aberrations-in-f1-placentas-fathered-by-obese-males
#11
Megan Mitchell, Reiner Strick, Pamela L Strissel, Ralf Dittrich, Nicole O McPherson, Michelle Lane, Galyna Pliushch, Ramya Potabattula, Thomas Haaf, Nady El Hajj
Gene expression and/or epigenetic deregulation may have consequences for sperm and blastocysts, as well as for the placenta, together potentially contributing to problems observed in offspring. We previously demonstrated specific perturbations of fertilization, blastocyst formation, implantation, as well as aberrant glucose metabolism and adiposity in offspring using a mouse model of paternal obesity. The current investigation analysed gene expression and methylation of specific CpG residues in F1 placentas of pregnancies fathered by obese and normal-weight male mice, using real-time PCR and bisulfite pyrosequencing...
February 10, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28181558/an-air-liquid-interphase-approach-for-modeling-the-early-embryo-maternal-contact-zone
#12
S Chen, S E Palma-Vera, M Langhammer, S P Galuska, B C Braun, E Krause, A Lucas-Hahn, J Schoen
We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional epithelial tissues, which generate oviduct fluid surrogates. These in vitro oviduct epithelia can be co-cultured with living zygotes and enable embryonic development up to the blastocyst stage without addition of embryo culture medium. The described strategy is broadly applicable to analyze early embryo-maternal interactions under standardized in vitro conditions.
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28176010/clinical-potentials-of-human-pluripotent-stem-cells
#13
REVIEW
Cristina Mora, Marialaura Serzanti, Antonella Consiglio, Maurizio Memo, Patrizia Dell'Era
Aging, injuries, and diseases can be considered as the result of malfunctioning or damaged cells. Regenerative medicine aims to restore tissue homeostasis by repairing or replacing cells, tissues, or damaged organs, by linking and combining different disciplines including engineering, technology, biology, and medicine. To pursue these goals, the discipline is taking advantage of pluripotent stem cells (PSCs), a peculiar type of cell possessing the ability to differentiate into every cell type of the body. Human PSCs can be isolated from the blastocysts and maintained in culture indefinitely, giving rise to the so-called embryonic stem cells (ESCs)...
February 8, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28171747/the-apical-domain-is-required-and-sufficient-for-the-first-lineage-segregation-in-the-mouse-embryo
#14
Ekaterina Korotkevich, Ritsuya Niwayama, Aurélien Courtois, Stefanie Friese, Nicolas Berger, Frank Buchholz, Takashi Hiiragi
Mammalian development begins with segregation of the extra-embryonic trophectoderm from the embryonic lineage in the blastocyst. While cell polarity and adhesion play key roles, the decisive cue driving this lineage segregation remains elusive. Here, to study symmetry breaking, we use a reduced system in which isolated blastomeres recapitulate the first lineage segregation. We find that in the 8-cell stage embryo, the apical domain recruits a spindle pole to ensure its differential distribution upon division...
February 6, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28169188/clinical-outcomes-after-ivf-or-icsi-using-human-blastocysts-derived-from-oocytes-containing-aggregates-of-smooth-endoplasmic-reticulum
#15
Fumiaki Itoi, Yukiko Asano, Masashi Shimizu, Rika Nagai, Kanako Saitou, Hiroyuki Honnma, Yasutaka Murata
In this study the clinical and neo-natal outcomes after transfer of blastocysts derived from oocytes containing aggregates of smooth endoplasmic reticulum (SER) were compared between IVF and intracytoplasmic sperm injection (ICSI) cycles. Clinical and neo-natal outcomes of blastocysts in cycles with at least one SER metaphase II oocyte (SER + MII; SER + cycles) did not significantly differ between the two insemination methods. When SER + MII were cultured to day 5/6, fertilization, embryo cleavage and blastocyst rates were not significantly different between IVF and ICSI cycles...
January 25, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28166990/use-of-fsh-in-two-different-regimens-for-ovarian-superstimulation-prior-to-ovum-pick-up-and-in%C3%A2-vitro-embryo-production-in-holstein-cows
#16
Júlio César Barboza da Silva, Roberta Machado Ferreira, Milton Maturana Filho, Julianne de Rezende Naves, Thiago Santin, Guilherme Pugliesi, Ed Hoffmann Madureira
We aimed with the present study to evaluate the effects of FSH treatment (200 mg) split in four or six administrations on ovarian follicle stimulation and in vitro oocyte competence for embryo production in dairy cows with synchronized follicular wave emergence. On random days of the estrous cycle (Day 0), non-lactating Holstein cows received a progesterone (P4)-releasing intravaginal device and 2 mg estradiol benzoate IM. On Day 3, they received 0.530 mg sodium cloprostenol (PGF2α) IM. Control cows (n = 35) received no further treatments, whereas FSH-treated cows received 200 mg FSH split in four (FSH4 group; n = 33) or six (FSH6 group; n = 33) administration regimens...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28166988/beef-donor-cows-with-high-number-of-retrieved-coc-produce-more-in%C3%A2-vitro-embryos-compared-with-cows-with-low-number-of-coc-after-repeated-ovum-pick-up-sessions
#17
F M Monteiro, E O S Batista, L M Vieira, B M Bayeux, M Accorsi, S P Campanholi, E A R Dias, A H Souza, P S Baruselli
The aim of the present study was to evaluate whether repeated ovum pick-up (OPU) procedures may affect the efficiency of OPU programs for in vitro embryo production (IVEP) in Bos indicus (Nelore) donors. In addition, the repeatability (r) efficiency of IVEP was also assessed. Data available were from 432 OPU-IVEP sessions that were performed at random stages of the estrous cycle in 36 cycling, nonlactating Nelore donors. Semen from three Nelore bulls was used for the IVF. Donors were submitted to 12 consecutive OPU procedures, with an interval of approximately 30 days between sessions...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28166979/peroxisome-proliferator-activated-receptor-%C3%AE-improves-porcine-blastocyst-hatching-via-the-regulation-of-fatty-acid-oxidation
#18
Jing Guo, Wen-Fa Lu, Shuang Liang, Jeong-Woo Choi, Nam-Hyung Kim, Xiang-Shun Cui
Peroxisome proliferator-activated receptor δ (Pparδ) is a nuclear receptor that plays critical roles in lipid metabolism, glucose metabolism, and cell growth and differentiation. Several recent studies have shown that Pparδ promotes blastocyst hatching in vitro. However, the mechanism by which it promotes preimplantation embryonic development in vitro remains unclear. In this study, oocytes and parthenotes were treated with a specific agonist of PPARδ, GW501516. The activation of PPARδ had no effect on oocyte maturation for 1 μM and 10 μM GW501516 compared with the control group...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28166968/interspecies-somatic-cell-nuclear-transfer-in-asiatic-cheetah-using-nuclei-derived-from-post-mortem-frozen-tissue-in-absence-of-cryo-protectant-and-in%C3%A2-vitro-matured-domestic-cat-oocytes
#19
F Moulavi, S M Hosseini, N Tanhaie-Vash, S Ostadhosseini, S H Hosseini, M Hajinasrollah, M H Asghari, H Gourabi, A Shahverdi, A D Vosough, M H Nasr-Esfahani
Recent accomplishments in the field of somatic cell nuclear transfer (SCNT) hold tremendous promise to prevent rapid loss of animal genetic resources using ex situ conservation technology. Most of SCNT studies use viable cells for nuclear transfer into recipient oocytes. However, preparation of live cells in extreme circumstances, in which post-mortem material of endangered/rare animals is improperly retained frozen, is difficult, if not impossible. This study investigated the possibility of interspecies-SCNT (iSCNT) in Asiatic cheetah (Acinonyx jubatus venaticus), a critically endangered subspecies, using nuclei derived from frozen tissue in absence of cryo-protectant at -20 °C and in vitro matured domestic cat oocytes...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28166962/expression-and-localization-of-artemin-in-the-bovine-uterus-and-embryos
#20
E Gómez, D Martin, S Carrocera, M J Sánchez-Calabuig, A Gutierrez-Adán, M Alonso-Guervos, N Peynot, C Giraud-Delville, O Sandra, V Duranthon, M Muñoz
Artemin a member of the glial cell line-derived neurotrophic factor (GDNF) family is present in mice and human preimplantation embryos, and reproductive tract, during early pregnancy promoting embryo development in vitro. The presence of artemin in cattle embryos and reproductive tract, however, is unknown. In the present work we identified for first time artemin in bovine uterine fluid (UF) (Western blot), endometrium (RT-PCR, Western blot and immunohistochemistry) and embryos (RT-PCR and immunohistochemistry) during early preimplantation development...
March 1, 2017: Theriogenology
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