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https://www.readbyqxmd.com/read/28545999/a-comparative-analysis-of-the-efficacy-of-three-cryopreservation-protocols-on-the-survival-of-in-vitro-derived-cattle-embryos-at-pronuclear-and-blastocyst-stages
#1
Van Huong Do, Simon Walton, Sally Catt, Andrew W Taylor-Robinson
The effectiveness of three cryopreservation protocols (slow freezing, short equilibration vitrification and long equilibration vitrification) on in vitro-derived cattle embryos at expanded blastocyst and pronuclear stages was compared. 199 expanded blastocysts of good quality were assigned randomly into four treatment groups [control, non-cryopreserved (fresh, unfrozen); and the three cryopreservation methods]. The re-expansion of the cryopreserved blastocysts after 24 h in vitro culture was similar to that of the fresh control group...
May 22, 2017: Cryobiology
https://www.readbyqxmd.com/read/28545049/first-cloned-bactrian-camel-camelus-bactrianus-calf-produced-by-interspecies-somatic-cell-nuclear-transfer-a-step-towards-preserving-the-critically-endangered-wild-bactrian-camels
#2
Nisar Ahmad Wani, Binoy S Vettical, Seung B Hong
Studies were conducted to explore the possibility of employing dromedary camel (Camelus dromedarius) oocytes as recipient cytoplasts for the development of interspecies somatic cell nuclear transfer (iSCNT) embryos using skin fibroblast cells of an adult Bactrian camel (Camelus bactrianus) and Llama (Llama glama) as donor nuclei. Also, the embryos reconstructed with Bactrian cells were transferred into the uterus of synchronized dromedary camel recipients to explore the possibility of using them as surrogate mothers...
2017: PloS One
https://www.readbyqxmd.com/read/28543962/effects-of-meiotic-inhibitors-and-gonadotrophins-on-porcine-oocytes-in-vitro-maturation-fertilization-and-development
#3
M A Gil, A Nohalez, C A Martinez, J R Ake-Villanueva, F Centurion-Castro, C Maside, C Cuello, J Roca, I Parrilla, E A Martinez
This study evaluated the effect of three reversible meiotic inhibitors (MINs) and their interaction with gonadotrophins (Gns) on the meiotic maturation and developmental competence of porcine oocytes. In experiment 1, the oocytes were matured for 22 hr in the presence or absence of dbcAMP (1 mM), cycloheximide (7 μM) or cilostamide (20 μM) with or without Gns, and for an additional 22 hr in the absence of MINs and Gns. At 22 hr of maturation, regardless of the presence of Gns, a higher proportion (p < ...
May 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28543358/transient-arrest-of-germinal-vesicle-breakdown-improved-in-vitro-development-potential-of-buffalo-bubalus-bubalis-oocytes
#4
Sandeep Kumar, Manish Kumar, Sunny Dholpuria, Parul Sarwalia, Vipul Batra, Sachinandan De, Rakesh Kumar, T K Datta
Germinal vesicle breakdown (GVBD) is the first milestone that an oocyte needs to achieve towards completing the maturation and gaining potential to fertilise. Significantly lower in vitro embryo production rate in buffaloes can be attributed to heterogeneity of GVBD occurrence among oocytes obtained from abattoir derived ovaries. Evidence from our earlier work had suggested that different qualities of buffalo oocytes differ significantly in their timing of GVBD. Besides, these oocytes also differ in terms of volume of Akt phosphorylation, which initiates the process of GVBD...
May 25, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28542635/frap-analysis-of-chromatin-looseness-in-mouse-zygotes-that-allows-full-term-development
#5
Masatoshi Ooga, Teruhiko Wakayama
Chromatin looseness, which can be analyzed by fluorescence recovery after photobleaching (FRAP) using eGFP-tagged core histone proteins, is an important index of the differentiation potential of blastomere cells and embryonic stem cells. Whether chromatin looseness is a reliable index of the developmental potential of embryos during ontogenesis is not known. As a necessary first step toward answering this question, we investigated whether FRAP-analyzed embryos are capable of normal preimplantation and full-term development...
2017: PloS One
https://www.readbyqxmd.com/read/28542629/characteristics-of-candidate-genes-associated-with-embryonic-development-in-the-cow-evidence-for-a-role-for-wbp1-in-development-to-the-blastocyst-stage
#6
M Sofia Ortega, Justin J Kurian, Robert McKenna, Peter J Hansen
The goal was to gain understanding of how 12 genes containing SNP previously related to embryo competence to become a blastocyst (BRINP3, C1QB, HSPA1L, IRF9, MON1B, PARM1, PCCB, PMM2, SLC18A2, TBC1D24, TTLL3 and WBP1) participate in embryonic development. Gene expression was evaluated in matured oocytes and embryos. BRINP3 and C1QB were not detected at any stage. For most other genes, transcript abundance declined as the embryo developed to the blastocyst stage. Exceptions were for PARM1 and WBP1, where steady-state mRNA increased at the 9-16 cell stage...
2017: PloS One
https://www.readbyqxmd.com/read/28542562/identification-and-characteristics-of-extracellular-vesicles-from-bovine-blastocysts-produced-in-vitro
#7
Edwin A Mellisho, Alejandra E Velásquez, María J Nuñez, Joel G Cabezas, Juan A Cueto, Claudio Fader, Fidel O Castro, Lleretny Rodríguez-Álvarez
Extracellular vesicles (EVs) have been identified within different body fluids and cell culture media. However, there is very little information on the secretion of these vesicles during early embryonic development. The aims of this work were first to demonstrate the secretion of extracellular vesicles by pre-implantation bovine embryos and second to identify and characterize the population of EVs secreted by bovine blastocysts during the period from day seven to nine of embryo culture and its correlation with further embryo development up to day 11...
2017: PloS One
https://www.readbyqxmd.com/read/28542262/the-role-of-the-innate-immune-response-regulatory-gene-abcf1-in-mammalian-embryogenesis-and-development
#8
Sara M Wilcox, Hitesh Arora, Lonna Munro, Jian Xin, Franz Fenninger, Laura A Johnson, Cheryl G Pfeifer, Kyung Bok Choi, Juan Hou, Pamela A Hoodless, Wilfred A Jefferies
ABCF1 is an ABC transporter family protein that has been shown to regulate innate immune response and is a risk gene for autoimmune pancreatitis and arthritis. Unlike other members of ABC transporter family, ABCF1 lacks trans-membrane domains and is thought to function in translation initiation through an interaction with eukaryotic translation initiation factor 2 (eIF2). To study ABCF1 expression and function in development and disease, we used a single gene trap insertion in the Abcf1 gene in murine embryonic stem cells (ES cells) that allowed lineage tracing of the endogenous Abcf1 promoter by following the expression of a β-galactosidase reporter gene...
2017: PloS One
https://www.readbyqxmd.com/read/28540437/volatile-organic-compounds-and-good-laboratory-practices-in-the-in-vitro-fertilization-laboratory-the-important-parameters-for-successful-outcome-in-extended-culture
#9
Nupur Agarwal, Ratna Chattopadhyay, Sanghamitra Ghosh, Arpita Bhoumik, S K Goswami, Baidyanath Chakravarty
PURPOSE: This study aims to describe the role of implementing good laboratory practices to improve in vitro fertilization (IVF) outcomes which are of great interest for practitioners dealing with infertility. METHODS: Certain modifications were introduced in May 2015 in our IVF laboratory like high-efficiency particulate air CODA system, steel furniture instead of wooden, use of new disinfectants like oosafe, and restriction of personnel entry along with avoidance of cosmetics like perfume to improve pregnancy rates...
May 24, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28537488/capturing-human-na%C3%A3-ve-pluripotency-in-the-embryo-and-in-the-dish
#10
Ludovic Zimmerlin, Tea Soon Park, Elias T Zambidis
Although human embryonic stem cells (hESC) were first derived almost 20 years ago, it was only recently acknowledged that they share closer molecular and functional identity to post-implantation lineage-primed murine epiblast stem cells (EpiSC) than to naïve pre-implantation ICM-derived mouse embryonic stem cells (ESC). A myriad of transcriptional, epigenetic, biochemical, and metabolic attributes have now been described that distinguish naïve and primed pluripotent states in both rodents and humans. Conventional hESC and human induced pluripotent stem cells (hiPSC) appear to lack many of the defining hallmarks of naïve mouse ESC...
May 24, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28532843/expression-and-regulation-of-interleukin-6-and-its-receptor-at-the-maternal-conceptus-interface-during-pregnancy-in-pigs
#11
Inkyu Yoo, Jisoo Han, Minjeong Kim, Hwanhee Jang, Soojin Sa, Sun-Ho Choi, Hakhyun Ka
It has been well established that interleukin 6 (IL6), a pleiotropic cytokine with multiple functional roles, is widely expressed in the female reproductive tract and mediates blastocyst implantation and placental development in many species. Uterine expression of IL6 during early pregnancy has been studied in pigs, but expression and function of IL6 at the maternal-placental interface throughout pregnancy have not been determined. Thus, we examined expression of IL6 and its receptors, IL6 receptor (IL6R) and GP130, in the uterine endometrium on Days 12 and 15 of the estrous cycle, and Days 12, 15, 30, 60, 90, and 114 of pregnancy, conceptus on Days 12 and 15, and chorioallantoic tissues on Days 30, 60, 90, and 114 of pregnancy in pigs...
July 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28532827/hepatoma-derived-growth-factor-protein-quantification-in-uterine-fluid-gene-expression-in-endometrial-cell-culture-and-effects-on-in%C3%A2-vitro-embryo-development-pregnancy-and-birth
#12
E Gómez, S Carrocera, D Martin, M J Sánchez-Calabuig, A Gutiérrez-Adán, A Murillo, M Muñoz
Hepatoma-derived growth factor (HDGF) is present in the endometrium of cows and other mammals. Recombinant HDGF (rHDGF) improves bovine blastocyst development in vitro. However, specific culture conditions and essential aspects of HDGF uterine physiology are yet unknown. In this work we quantified total HDGF protein in uterine fluid (UF) by multiple reaction monitoring (MRM), and analyzed effects of rHDGF on specific embryonic stages with Day-6 bovine embryos cultured in vitro with and without BSA, and on pregnancy viability and calf phenotypes after embryo transfer to recipients...
July 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28532595/impact-of-elemental-iron-on-human-spermatozoa-and-mouse-embryonic-development-in-a-defined-synthetic-culture-medium
#13
Safaa M Naes, Oshini Basri, Fauziah Ismail, Ghofraan A Ata'Allah, Siti Khadijah Idris, Noor Azmi Mat Adenan, Jaffar Ali
There is a paucity of studies on effect of iron in embryo culture procedures. This study aims to ascertain the optimal, tolerance and toxic levels of iron in a protein-free embryo culture medium (PFM) to determine the effect of iron on embryonic development. The application of PFM in assisted reproductive technologies (ART) is intended to eliminate disease transmission and improve ART treatment outcome. The optimal, tolerance and toxic levels of iron on human spermatozoa and mouse embryos were determined by challenging them with different levels of iron (ferric iron; Fe(+3))...
May 19, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28531193/raman-microscopy-investigation-of-vitrification-induced-structural-damages-in-mature-bovine-oocytes
#14
Giulia Rusciano, Carolina De Canditiis, Gianluigi Zito, Marcello Rubessa, Maria Serena Roca, Rosa Carotenuto, Antonio Sasso, Bianca Gasparrini
Although oocyte cryopreservation has great potentials in the field of reproductive technologies, it still is an open challenge in the majority of domestic animals and little is known on the biochemical transformation induced by this process in the different cellular compartments. Raman micro-spectroscopy allows the non-invasive evaluation of the molecular composition of cells, based on the inelastic scattering of laser photons by vibrating molecules. The aim of this work was to assess the biochemical modifications of both the zona pellucida and cytoplasm of vitrified/warmed in vitro matured bovine oocytes at different post-warming times...
2017: PloS One
https://www.readbyqxmd.com/read/28526652/retinoic-acid-induces-differentiation-of-buffalo-bubalus-bubalis-embryonic-stem-cells-into-germ-cells
#15
Syed Mohmad Shah, Suresh Kumar Singla, Prabhat Palta, Radhey Sham Manik, Manmohan Singh Chauhan
Development of precise and reproducible culture system for in vitro differentiation of embryonic stem (ES) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (PGCs), but in finally treating the catastrophic infertility. Taking into account the significant role of retinoic acid (RA) during in vivo gametogenesis, we designed the present study to investigate the effects of its stimulation on directing the differentiation of ES cells into germ cells...
May 16, 2017: Gene
https://www.readbyqxmd.com/read/28526407/sirtuin-inhibition-leads-to-autophagy-and-apoptosis-in-porcine-preimplantation-blastocysts
#16
Min Gyeong Kim, Duk Hyoun Kim, Hye Ran Lee, Jun Sung Lee, Su Jin Jin, Hoon Taek Lee
Sirtuins are nicotinamide adenine dinucleotide dependent class III histone deacetylase proteins that play a crucial role in several cellular processes, including DNA repair, apoptosis, and lifespan. Previous studies have shown that sirtuin inhibition leads to embryonic developmental arrest and oxidative stress in porcine and murine. However, sirtuin-mediated mechanisms have not been examined in porcine preimplantation blastocysts. We therefore investigated the relationship between sirtuins and autophagy. Embryos were cultured with 100 μM sirtinol (SIRT1/2 inhibitor) in NCSU-23 media after in vitro fertilization...
May 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28525345/in-vitro-production-of-sudanese-camel-camelus-dromedarius-embryos-from-epididymal-spermatozoa-and-follicular-oocytes-of-slaughtered-animals
#17
A E Abdelkhalek, Sh A Gabr, W A Khalil, Sh M Shamiah, L Pan, G Qin, M H Farouk
Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter)...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28525327/in-vitro-fertilizing-potential-of-urethral-and-epididymal-spermatozoa-collected-from-domestic-cats-felis-catus
#18
S Prochowska, W Niżański
The aim of this study was to provide a comparative analysis of in vitro fertilizing potential of frozen-thawed urethral and epididymal feline spermatozoa. Both types of semen were collected from 7 cats and cryopreserved in liquid nitrogen. To perform in vitro fertilization, both urethral and epididymal samples from the same individual were thawed and spermatozoa were co-incubated with in vitro matured cat oocytes. Obtained embryos were cultured in vitro for 7 days in a commercial medium. Cleavage rate, morula rate and blastocyst rate were calculated...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28523122/sex-differences-in-rat-placental-development-from-pre-implantation-to-late-gestation
#19
J I Kalisch-Smith, D G Simmons, M Pantaleon, K M Moritz
BACKGROUND: A male fetus is suggested to be more susceptible to in utero and birth complications. This may be due in part to altered morphology or function of the XY placenta. We hypothesised that sexual dimorphism begins at the blastocyst stage with sex differences in the progenitor trophectoderm (TE) and its derived trophoblast lineages, as these cells populate the majority of cell types within the placenta. We investigated sex-specific differences in cell allocation in the pre-implantation embryo and further characterised growth and gene expression of the placental compartments from the early stages of the definitive placenta through to late gestation...
2017: Biology of Sex Differences
https://www.readbyqxmd.com/read/28522355/closed-versus-open-vitrification-for-human-blastocyst-cryopreservation-a-meta-analysis
#20
Hyun Sik Youm, Jong-Ryeol Choi, Daesik Oh, Yong Ho Rho
Closed vitrification can minimize the risk of microbiological transmission through liquid nitrogen during the cooling, storage, and warming procedures. As cooling rates may reduce when closed vitrification is applied, clinical outcomes should be compared between closed and open vitrification in order to justify the use of closed vitrification. This study was conducted to investigate the differences in survival, implantation, clinical pregnancy, and live birth rates between closed and open vitrification for human blastocyst cryopreservation...
May 15, 2017: Cryobiology
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