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https://www.readbyqxmd.com/read/18600933/biocatalytic-synthesis-of-disaccharide-high-intensity-sweeterner-sucralose-via-a-tetrachlororaffinose-intermediate
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C Bennett, J S Dordick, A J Hacking, P S Cheetham
A second high-yielding bioorganic synthesis of the highintensity sweetener sucralose (4,1',6'-trichloro-4,1',6'-trideoxygalactosucrose) is described. This procedure involves the chemical chlorination of raffinose to form a novel tetrachloroaffinose intermediate (6,4',1'',6''-tetrachloro-6,4',1'',6''-tetradeoxygalactoraffinose; TCR) followed by the enzymic hydrolysis of the alpha-1-6 glycosidic bond of TCR to give sucralose and 6-chlorogalactose. Commercial enzyme preparations and microorganisms were screened to select alpha-galactosidases which have high catalytic activity on this compound...
January 20, 1992: Biotechnology and Bioengineering
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