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https://www.readbyqxmd.com/read/28432708/in-vitro-production-of-porcine-zygotes-using-intracytoplasmic-injection-of-vitrified-sperm
#1
C C Arraztoa, C Baca Castex, G M Alvarez, P D Cetica, D M Neild
The objectives of this study were to evaluate if vitrified porcine spermatozoa are able to maintain their capacity to produce zygotes in vitro using intracytoplasmic sperm injection (ICSI) and to evaluate the zygote development in two in vitro atmospheric conditions: 5% CO2 and tri-gas. A group of porcine oocytes maturated in vitro were injected with vitrified-warmed sperm (treatment group) and another group, with sperm diluted and conserved at 17°C (control group). To evidence parthenogenetic activation, some oocytes were submitted to a Sham test...
April 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28431828/clinical-use-of-monopronucleated-zygotes-following-blastocyst-culture-and-preimplantation-genetic-screening-including-verification-of-biparental-chromosome-inheritance
#2
Cara K Bradley, Maria Traversa, Natalie Hobson, Alison J Gee, Steven J McArthur
In assisted reproduction, embryos derived from monopronucleated (1PN) zygotes are considered abnormal and unsuitable for clinical use. Outcomes of 1PN-derived embryos designated for preimplantation genetic screening (PGS) were analysed. These embryos, especially from intracytoplasmic sperm injection (ICSI), were found to have a low developmental potential; 1PN and 2PN day 5 blastocyst development for IVF was 14.8% versus 36.4% (P < 0.0001), and for ICSI, 6.6% versus 34.0% (P < 0.0001), respectively. With the use of comparative genomic hybridization or next-generation sequencing, PGS was successfully carried out for 74 IVF and 32 ICSI 1PN-derived blastocysts, revealing adjusted abnormality rates of 39...
March 27, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28429340/controlled-prospective-observational-study-on-the-efficiency-and-tolerability-of-a-combination-of-potential-nrf2-inducing-antioxidants-and-micronutrients-as-pre-treatment-for-icsi-in-dyspermic-patients-with-previous-failure
#3
R Rago, M Gallo, A Dal Lago, E Licata, G Paciotti, M Amodei, C Meneghini, C Fabiani, G Dani, C Liberanome, D Antonaci, R Corno, D Miriello, G Giuffrida, B Giammusso
OBJECTIVE: To evaluate the efficiency of pre-treatment in dyspermic males in IVF couples with a combination of micronutrients, for the purpose of improving the fertilization rate, the implantation rate and the outcome of the pregnancy. PATIENTS AND METHODS: This controlled prospective clinical study was performed in two medically assisted reproduction centers. 59 males with mild oligo-astheno-teratospermia (OAT) were admitted to the study. All of them had a history of previous in vitro fertilization (IVF) attempts with female partners aged < 40 diagnosed having tubal or idiopathic infertility...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28428944/the-status-of-assisted-reproductive-technology-in-korea-in-2012
#4
Gyoung Hoon Lee, Hyun Jin Song, Young Min Choi, Hyuck Dong Han
OBJECTIVE: This study was designed to report the status of assisted reproductive technology (ART) therapy in South Korea between January 1, 2012 and December 31, 2012. METHODS: A localized online survey, originally developed by the International Committee Monitoring Assisted Reproductive Technologies, was first launched and provided to all available ART centers via email in 2015. Fresh embryo transfer (FET) cases were categorized as standard in vitro fertilization, intracytoplasmic sperm injection (ICSI), or half-ICSI...
March 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28428942/follicular-fluid-cerebellin-and-betatrophin-regulate-the-metabolic-functions-of-growing-follicles-in-polycystic-ovary-syndrome
#5
Aynur Adeviye Ersahin, Mustafa Acet, Suat Suphan Ersahin, Tuba Acet, Meltem Yardim, Omer Kenanoglu, Suleyman Aydin
OBJECTIVE: The aim of this study was to assess the changes of follicular fluid (FF) and serum levels of cerebellin precursor protein 1 (cbln1) and betatrophin in patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) with a gonadotropin-releasing hormone (GnRH) antagonist protocol. METHODS: Twenty infertile women with PCOS and 20 control women diagnosed as poor responders undergoing ovarian stimulation with a GnRH antagonist were included...
March 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28422964/effect-of-women-s-age-on-embryo-morphology-cleavage-rate-and-competence-a-multicenter-cohort-study
#6
Marie Louise Grøndahl, Sofie Lindgren Christiansen, Ulrik Schiøler Kesmodel, Inge Errebo Agerholm, Josephine Gabriela Lemmen, Peter Lundstrøm, Jeanette Bogstad, Morten Raaschou-Jensen, Steen Ladelund
This multicenter cohort study on embryo assessment and outcome data from 11,744 IVF/ICSI cycles with 104,830 oocytes and 42,074 embryos, presents the effect of women's age on oocyte, zygote, embryo morphology and cleavage parameters, as well as cycle outcome measures corrected for confounding factors as center, partner's age and referral diagnosis. Cycle outcome data confirmed the well-known effect of women's age. Oocyte nuclear maturation and proportion of 2 pro-nuclear (2PN) zygotes were not affected by age, while a significant increase in 3PN zygotes was observed in both IVF and ICSI (p<0...
2017: PloS One
https://www.readbyqxmd.com/read/28422380/comparing-the-long-acting-and-short-acting-forms-of-gonadotropin-releasing-hormone-agonists-in-the-long-protocol-of-ivf-icsi-cycles-a-retrospective-study
#7
Liyan Duan, Shihua Bao, Kunming Li, Xiaoming Teng, Ling Hong, Xiaoyu Zhao
AIM: This study aimed to compare the efficacy of long- and short-acting gonadotropin-releasing hormone agonist on clinical outcomes of in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) long protocol cycles. METHODS: In this retrospective study, 478 patients were enrolled from October 2012 to November 2014. The pituitary downregulation result, serum hormone levels, gonadotropin (Gn) dose during controlled ovarian hyperstimulation, and outcome of IVF/ICSI-embryo transfer were compared between the two groups...
April 19, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28421599/surgery-versus-conservative-management-of-endometriomas-in-subfertile-women-a-systematic-review
#8
Jacob Brink Laursen, Jeppe Bennekou Schroll, Kirsten T Macklon, Martin Rudnicki
INTRODUCTION: Endometriomas are present in up to 44% of all women with endometriosis and have a detrimental effect on fertility. However, it is controversial whether endometriomas should be surgically removed before assisted reproduction technology (ART). Our purpose was to evaluate whether surgical stripping of endometriomas in subfertile women improves the chance of a live birth. Secondary outcomes were impact on ovarian reserve and pain. Material and methods We conducted a systematic review and metaanalysis with results reported in accordance to the PRISMA guidelines...
April 19, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28421491/chapter-7-human-epididymal-and-testicular-sperm-cryopreservation
#9
Pankaj Talwar, Sarabpreet Singh
Since the advent of intracytoplasmic sperm injection (ICSI) in the early 1990s, surgical techniques to recover samples from the epididymis and testis directly have been used to benefit patients suffering from obstructive and nonobstructive azoospermia. Various studies have demonstrated comparable fertilization, ongoing pregnancy, and implantation rates when fresh and frozen-thawed epididymal sperms were used for ICSI [1]. Injection of fresh and frozen testicular sperms into mature oocytes resulted in similar fertilization rates in cases of obstructive azoospermia...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421490/chapter-6-technology-of-aseptic-cryoprotectant-free-vitrification-of-human-icsi-spermatozoa
#10
Vladimir Isachenko, Raul Sanchez, Peter Mallmann, Gohar Rahimi, Evgenia Isachenko
The aim of this chapter was to describe the standardized aseptic technology of permeable cryoprotectant-free vitrification of human spermatozoa in capillaries (for ICSI or IVF in microvolume). Spermatozoa, vitrified by this technology, are free from seminal plasma owing to swim-up procedure preceding vitrification and are free from permeable cryoprotectants. They are ready for further use immediately after warming without any additional treatment.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28420500/does-different-bmi-influence-oocyte-and-embryo-quality-by-inducing-fatty-acid-in-follicular-fluid
#11
Parvaneh Mirabi, Mohammad Javad Chaichi, Sedighe Esmaeilzadeh, Seyed Gholam Ali Jorsaraei, Ali Bijani, Mahjobeh Ehsani
OBJECTIVE: We aimed to assess the effects of obesity-related follicular fluid (FF) fatty acids (FAs) on the number and quality of oocytes, good embryo quality rate, and pregnancy rate. MATERIALS AND METHODS: This prospective cohort study was conducted on 105 infertile women under the age of 38, who underwent intracytoplasmic sperm injection (ICSI) from March 2015 to October 2015. They were grouped into three body mass index (BMI) categories. The fatty acids composition of the FF was analyzed by GC-MS head space method...
April 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28419910/endometriosis-doubles-odds-for-miscarriage-in-patients-undergoing-ivf-or-icsi
#12
Corinna Pallacks, Jens Hirchenhain, Jan-Steffen Krüssel, Tanja N Fehm, Daniel Fehr
OBJECTIVE: To identify and estimate the importance of risk factors on pregnancy loss until the end of the second trimester after clinical pregnancy was achieved by either in vitro fertilisation (IVF) or intra-cytoplasmic sperm injection (ICSI). STUDY DESIGN: Retrospective cohort study including 588 cycles with fresh embryo transfers and 150 cycles with frozen-thawed embryo transfers using logistic regression. RESULTS: The rate of miscarriages subsequent to a fresh embryo transfer was significantly increased by a diagnosis of endometriosis (p=0...
April 5, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28419502/nuchal-translucency-measurement-free-%C3%AE-hcg-and-papp-a-concentrations-in-ivf-icsi-pregnancies-systematic-review-and-meta-analysis
#13
REVIEW
Paolo Cavoretto, Veronica Giorgione, Sonia Cipriani, Paola Viganò, Massimo Candiani, Annalisa Inversetti, Elena Ricci, Fabio Parazzini
So far data on the effect of assisted reproductive technologies (ART) on the components of first trimester combined screening for Down syndrome are still controversial. A systematic search of the literature was performed in order to identify the effect of ART, particularly IVF and ICSI with fresh embryo transfer, on the nuchal translucency (NT), free β-hCG and PAPP-A measurements. Moreover, a meta-analysis and a descriptive graphical representation of the ratios between ART and spontaneous pregnancies (controls) values of median of the multiple of median (m0MOM) was performed...
April 17, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/28417052/relationship-between-meiotic-spindles-visualization-and-intracytoplasmic-sperm-injection-outcomes-in-human-oocytes
#14
Elham Asa, Reza Tabatabaee, Ahmad Farrokhi, Reza Nejatbakhsh
In assisted reproductive techniques, the operator attempts to select morphologically best embryos to predict embryo viability. Development of polarized light microscope, which evaluates the oocytes' spindles according to birefringence of living cells, had been helpful in oocyte selection. The aim of this study is evaluating the relationship between meiotic spindles visualization and intracytoplasmic sperm injection (ICSI) outcomes in human oocytes. In this study, 264 oocytes from 24 patients with an average age of 30...
March 2017: Anatomy & Cell Biology
https://www.readbyqxmd.com/read/28415952/effective-treatment-of-ketamine-associated-cystitis-with-botulinum-toxin-type-a-injection-combined-with-bladder-hydrodistention
#15
Jianfeng Zeng, Haibiao Lai, Dongxiang Zheng, Liang Zhong, Zhifeng Huang, Shanyun Wang, Weiwei Zou, Lianbo Wei
Objective Ketamine-associated cystitis (KAC) has been described in a few case reports, but its treatment in a relatively large number of patients has not been documented. This study aimed to describe our experience of treatment of 36 patients with KAC. Methods Thirty-six patients (30 males and 6 females, aged 19-38 years) with KAC, who had previously taken a muscarinic receptor blocker and/or antibiotics, but without symptomatic relief, were treated with botulinum toxin A injection combined with bladder hydrodistention...
April 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28407512/impact-of-large-ovarian-endometriomas-on-the-response-to-superovulation-for-in-vitro-fertilization-a-retrospective-study
#16
Simone Ferrero, Carolina Scala, Emanuela Tafi, Annalisa Racca, Pier Luigi Venturini, Umberto Leone Roberti Maggiore
OBJECTIVE: To assess the response to superovulation for in vitro fertilization (IVF) in patients with unilateral endometriomas with diameter ≥5cm and in the contralateral healthy ovary. STUDY DESIGN: This retrospective analysis of a prospectively collected database included patients who underwent superovulation for IVF/ICSI cycles and had unoperated single unilateral endometrioma with diameter ≥5cm and healthy contralateral ovary. The primary outcome of the study was to compare the number of oocyte retrieved in the ovary with the endometrioma and the contralateral healthy ovary...
April 2, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28405942/first-successful-conception-induced-by-a-male-cystinosis-patient
#17
Koenraad R Veys, Kathleen W D'Hauwers, Angelique J C M van Dongen, Mirian C Janssen, Martine T P Besouw, Ellen Goossens, Lambert P van den Heuvel, Alex A M M Wetzels, Elena N Levtchenko
Cystinosis is a rare autosomal recessive lysosomal storage disease characterized by multi-organ cystine accumulation, leading to renal failure and extra-renal organ dysfunction. Azoospermia of unknown origin is the main cause of infertility in all male cystinosis patients. Although spermatogenesis has shown to be intact at the testicular level in some patients, no male cystinosis patient has been reported yet to have successfully induced conception.We present the first successful conception ever reported, induced by a 27-year-old male renal transplant infantile nephropathic cystinosis patient through percutaneous epididymal sperm aspiration (PESA) followed by intracytoplasmatic sperm injection (ICSI)...
April 13, 2017: JIMD Reports
https://www.readbyqxmd.com/read/28405121/gnrh-agonist-treatment-improves-implantation-and-pregnancy-rates-of-frozen-thawed-embryos-transfer
#18
S A Hebisha, H M Adel
OBJECTIVE: To study the effect of GnRh agonist administration prior to estrogen-progesterone preparation of the endometrium on the implantation rate in frozen-thawed embryo transfer (FET) cycles in infertile patients treated with IVF/ICSI. DESIGN: Prospective controlled study. SETTING: Private center in Alexandria, Egypt. PATIENTS: Patients undergoing frozen-thawed embryo transfer FET. INTERVENTIONS: Patients were divided into two groups, A and B...
April 2017: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/28400828/effect-of-intrauterine-injection-of-human-chorionic-gonadotropin-before-embryo-transfer-on-pregnancy-rate-a-prospective-randomized-study
#19
Fatemeh Mostajeran, Farzaneh Godazandeh, Sayed Mehdi Ahmadi, Minoo Movahedi, Seyed Abolfazl Jabalamelian
BACKGROUND: Human chorionic gonadotropin (hCG) as the most important factor to controlled implantation is one of the early embryonic signals in primates that is secreted by the embryo before its implantation. This study was designed to assess the effects of intrauterine injection of hCG before the embryo transfer in an in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycle on pregnancy rate in infertile patients. MATERIALS AND METHODS: This randomized study was done on 100 infertile patients in two groups: intervention group received injection of 700 IU of intrauterine hCG 10 min before embryo transfer and control group did not receive hCG...
2017: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
https://www.readbyqxmd.com/read/28394112/effect-of-familial-mediterranean-fever-on-ivf-outcome-a-retrospective-case-series
#20
Nafiye Karakas Yilmaz, Mustafa Kara, Metin Kaba, Bugra Coskun, Selcuk Erkilinc, Salim Erkaya
Although the in vitro fertilization-intra-cytoplasmic sperm injection (IVF-ICSI) has been utilized widely, the management in patients with an autoimmune disease is still a challenge. The aim of this study was to demonstrate IVF-ICSI outcomes in infertile women with familial Mediterranean fever (FMF). Patient data were collected from the cases registered from January 2006 until January 2014. A total of 6152 assisted reproductive technology (ART) cycles were analyzed retrospectively in the Ankara Zekai Tahir Burak Women’s Health Education and Research Hospital...
June 2016: Acta Clinica Croatica
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