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https://www.readbyqxmd.com/read/28228320/embryo-multinucleation-at-the-two-cell-stage-is-an-independent-predictor-of-intracytoplasmic-sperm-injection-outcomes
#1
Laurent Desch, Céline Bruno, Maxime Luu, Julie Barberet, Cécile Choux, Marjorie Lamotte, Emeline Schmutz, Paul Sagot, Patricia Fauque
OBJECTIVE: To determine the prognostic impact of the nuclear status at the two-cell stage on intracytoplasmic sperm injection (ICSI) outcomes. DESIGN: Retrospective study. SETTING: Hospital. PATIENT(S): Only ICSI cycles with time-lapse monitoring of transferred embryos with known implantation/delivery data from November 2012 to December 2014 were included. A total of 2,449 embryos were assessed for multinucleation rates at the two- and four-cell stage, and 608 transferred embryos were studied for ICSI outcomes...
January 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28222446/the-timing-of-embryo-transfer-catheter-removal-should-it-be-delayed-or-done-immediately-a-prospective-randomized-trial
#2
Belgin Devranoğlu, Özkan Özdamar, Yiğit Çakıroğlu, Mehmet Küçükbaş, Meryem K Eken, Emek Doğer
BACKGROUND/AIMS: Data on the timing of catheter removal technique following embryo transfer (ET) are quite limited. We aimed to compare the reproductive outcomes of intracytoplasmic sperm injection (ICSI)/ET cycles in which the transfer catheter was removed immediately with those in which the catheter was removed after a delay period and hereby to evaluate the impact that the time interval before removal of the catheter following embryo deposit may have on the fertility outcomes. METHODS: A prospective randomized study was designed...
February 22, 2017: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/28216912/pregnancy-outcomes-and-maternal-and-perinatal-complications-of-pregnancies-following-in-vitro-fertilization-intracytoplasmic-sperm-injection-using-own-oocytes-donor-oocytes-and-vitrified-embryos-a-prospective-follow-up-study
#3
Manish Banker, Vidhisha Mehta, Dipesh Sorathiya, Mira Dave, Sandeep Shah
BACKGROUND: Several global studies have assessed maternal and perinatal outcomes and complications with the type of embryo transfer (ET) following in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI). The present study assessed the incidence of maternal and perinatal outcomes and complications following IVF/ICSI associated with the type of embryo transferred. METHODOLOGY: A total of 2112 ETs were performed in 2092 female patients aged 21-50 years between January 1 and December 31, 2014 (Group A: Fresh ET using self-oocytes: 691; Group B: Fresh ET using donor oocytes: 810; and Group C: Thaw ET using vitrified-warmed embryos: 611)...
October 2016: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28215488/gonadotropin-releasing-hormone-agonist-gnrha-trigger-state-of-the-art
#4
REVIEW
C Dosouto, T Haahr, P Humaidan
GnRH agonist (GnRHa) trigger for final oocyte maturation in GnRH antagonist co-treated IVF/ICSI cycles significantly reduces the risk of ovarian hyperstimulation syndrome (OHSS). GnRHa trigger followed by modifications of the standard luteal phase support (modified luteal phase support) secures fresh transfer in the majority of patients with excellent reproductive outcomes. In freeze all cycles (segmented cycles) GnRHa trigger allows oocyte retrieval with a minimal risk of early onset OHSS and good reproductive outcomes in subsequent frozen thaw cycles...
February 16, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28210479/pregnant-after-assisted-reproduction-a-risk-pregnancy-is-born-18-years-perinatal-outcome-results-from-a-population-based-registry-in-flanders-belgium
#5
W Ombelet, G Martens, L Bruckers
BACKGROUND: Although the increased risk for perinatal morbidity and mortality of babies born after ART is largely attributed to a higher rate of multiple gestations, a significantly worse perinatal outcome for singleton pregnancies following ART compared to pregnancies after natural conception has been reported as well. Most studies only include IVF/ICSI pregnancies; studies describing the perinatal outcome of pregnancies after non-IVF assisted reproduction are scarce. METHODS AND MATERIALS: Population-based cohort study with three exposure groups: a study group of pregnancies (1) after ovarian stimulation (OS), with or without artificial insemination (AI), (2) after IFV or ICSI and (3) a naturally conceived (NC) comparison group...
December 2016: Facts, Views & Vision in ObGyn
https://www.readbyqxmd.com/read/28209499/blastocyst-development-after-intracytoplasmic-sperm-injection-of-equine-oocytes-vitrified-at-the-germinal-vesicle-stage
#6
Heloísa Siqueira Canesin, Joao Gatto Brom-de-Luna, Young-Ho Choi, Isabel Ortiz, Mouhamadou Diaw, Katrin Hinrichs
We evaluated the meiotic and developmental competence of GV-stage equine oocytes vitrified under different conditions. In a preliminary study, using dimethyl sulfoxide (D), ethylene glycol (EG) and sucrose (S) as cryoprotectants, the maturation rate was higher for cumulus-oocyte complexes (COCs) held overnight before vitrification (37%) than for those vitrified immediately (14%; P < 0.05). Thereafter, all COCs were held overnight before vitrification. In Experiment 1 we compared 1 min (1 m) and 4 min (4 m) exposure to vitrification and warming solutions; oocytes that subsequently matured were fertilized by ICSI...
February 13, 2017: Cryobiology
https://www.readbyqxmd.com/read/28202358/a-prospective-multicenter-double-blind-randomized-trial-of-bladder-instillation-of-liposome-formulation-onabotulinumtoxina-for-interstitial-cystitis-bladder-pain-syndrome
#7
Yao-Chi Chuang, Hann-Chorng Kuo
BACKGROUND: Intravesical instillation of liposomal-formulated botulinum toxin A (lipotoxin) has shown therapeutic effects on treatment of refractory overactive bladder without needle injections. We hereby assessed lipotoxin for the treatment of refractory interstitial cystitis/bladder pain syndrome (IC/BPS). METHODS: This two-center, double-blind, randomized, placebo-controlled physician-initiated study enrolled patients with refractory IC/BPS. The patients were assigned to intravesical instillation of lipotoxin (onabotulinumtoxinA 200 U with 80mg sphingomyelin) (n=31), onabotulinumtoxinA 200 U in normal saline (n=28), or normal saline alone (n=31)...
February 12, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28202356/bladder-capacity-is-a-biomarker-for-a-bladder-centric-versus-systemic-manifestation-in-interstitial-cystitis-bladder-pain-syndrome
#8
Stephen J Walker, Joao Zambon, Karl-Erik Andersson, Carl Langefeld, Catherine A Matthews, Gopal Badlani, Heather Bowman, Robert J Evans
PURPOSE: Interstitial cystitis/bladder pain syndrome (IC/BPS) presents a significant clinical challenge due to symptom heterogeneity and the myriad associated comorbid medical conditions. We recently reported that diminished bladder capacity (BC) may represent a specific IC/BPS sub-phenotype. The objective of this study is to investigate the relationship between anesthetic bladder capacity and clinical findings (urologic and non-urologic) in a cohort of IC/BPS patients who had undergone therapeutic urinary bladder hydrodistention...
February 12, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28193084/value-of-endometrial-echopattern-at-hcg-administration-day-in-predicting-ivf-outcome
#9
Firoozeh Ahmadi, Farnaz Akhbari, Mina Zamani, Fariba Ramezanali, Rezvaneh Cheraghi
BACKGROUND: Assisted reproductive  treatment  (ART)  cycle  is an  expensive  procedure with low implantation and pregnancy rate; therefore, it is necessary to evaluate the predictors of success in these patients. OBJECTIVE: The relationship between endometrial echopattern and pregnancy rate was evaluated. METHODS: The endometrial  echopattern  was  analyzed  prospectively  on  the  day  of  human chorionic  gonadotropin  (HCG) administration  in  280  in  vitro  fertilization/intracytoplasmic  sperm  injection (IVF/ICSI) cycles with 8-14 mm endometrial thickness at Royan institute in 2013-2014...
February 2017: Archives of Iranian Medicine
https://www.readbyqxmd.com/read/28191261/global-dna-methylation-levels-are-altered-by-modifiable-clinical-manipulations-in-assisted-reproductive-technologies
#10
Jayashri Ghosh, Christos Coutifaris, Carmen Sapienza, Monica Mainigi
BACKGROUND: We analyzed placental DNA methylation levels at repeated sequences (LINE1 elements) and all CCGG sites (the LUMA assay) to study the effect of modifiable clinical or laboratory procedures involved in in vitro fertilization. We included four potential modifiable factors: oxygen tension during embryo culture, fresh embryo transfer vs frozen embryo transfer, intracytoplasmic sperm injection (ICSI) vs conventional insemination or day 3 embryo transfer vs day 5 embryo transfer...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28187532/testicular-sperm-aspiration-tesa-for-infertile-couples-with-severe-or-complete-asthenozoospermia
#11
A H Al-Malki, K Alrabeeah, E Mondou, V Brochu-Lafontaine, S Phillips, A Zini
The aim of the study was to evaluate reproductive outcomes in a cohort of infertile couples with severe and complete asthenozoospermia undergoing TESA (testicular sperm aspiration) with ICSI. We conducted a retrospective study of 28 couples with complete or severe asthenozoospermia who underwent TESA between January 2010 and December 2015. We compared TESA-ICSI outcomes of these couples to ejaculate ICSI outcomes of 40 couples with severe asthenozoospermia treated during the same time period at our institution...
February 10, 2017: Andrology
https://www.readbyqxmd.com/read/28187507/diagnostic-genetic-screening-for-assisted-reproductive-technologies-patients-with-macrozoospermia
#12
V Carmignac, J-M Dupont, R C Fierro, J Barberet, C Bruno, N Lieury, E Dulioust, J Auger, P Fauque
Macrozoospermia is characterized by a high proportion of abnormal spermatozoa with enlarged heads. So far, it has been associated with mutations only in the Aurora Kinase C gene (AURKC) in some cases. Although many publications have reported failure to conceive in couples with macrozoospermia, a few others have described successful pregnancies, thus raising questions as to whether ICSI and AURKC genetic screening should be recommended in all patients with macrozoospermia. First, we report on two monozygotic twins presenting macrozoospermia for whom the genetic status was explored (Aurora Kinase C sequencing) and whole semen and gradient-selected spermatozoa were analyzed, using Fluorescent In Situ Hybridization (FISH), Electron Microscopy and flow cytometry...
February 10, 2017: Andrology
https://www.readbyqxmd.com/read/28187504/extreme-spermatogenesis-failure-andrological-phenotype-and-intracytoplasmic-sperm-injection-outcomes
#13
P Plouvier, A-L Barbotin, F Boitrelle, D Dewailly, V Mitchell, J-M Rigot, V Lefebvre-Khalil, G Robin
Patients with very low sperm count through direct sperm examination can exhibit extreme oligozoospermia or cryptozoospermia (after centrifugation). The management of these patients is a real challenge for both clinicians and biologists. In this retrospective and comparative cohort study, we compared the andrological phenotype of patients with extreme alterations of spermatogenesis and assessed whether the origin of spermatozoa (testicular or ejaculate) had any influence on intracytoplasmic sperm injection (ICSI) outcomes...
February 10, 2017: Andrology
https://www.readbyqxmd.com/read/28186956/uterine-artery-doppler-pulsatility-index-at-11-38-weeks-in-icsi-pregnancies-with-egg-donation
#14
Annalisa Inversetti, Luca Mandia, Massimo Candiani, Irene Cetin, Alessandro Larcher, Valeria Savasi, Enrico Papaleo, Paolo Cavoretto
BACKGROUND: Uterine artery Doppler pulsatility index (UtA-PI) may be different in pregnancies with egg donation (ICSI-ED) as compared to conceptions with autologous intra-cytoplasmatic sperm injection (autologous ICSI) and to spontaneous conceptions (SC). METHODS: One hundred and ninety-four pregnant women with different modes of conception (MC) were prospectively evaluated: 53 ICSI-ED, 36 autologous ICSI and 105 SC. To evaluate the effects of different MC on PI, multivariable linear regression (MLR) models predicting UtA-PI were fitted after adjustment for maternal age, body mass index, race, parity, smoking status and gestational age...
February 10, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/28185121/the-association-between-coenzyme-q10-concentrations-in-follicular-fluid-with-embryo-morphokinetics-and-pregnancy-rate-in-assisted-reproductive-techniques
#15
Süleyman Akarsu, Funda Gode, Ahmet Zeki Isik, Zeliha Günnur Dikmen, Mustafa Agah Tekindağ
PURPOSE: This study seeks to evaluate the association between follicular fluid (FF) coenzyme Q10 (CoQ10) levels, embryo morphokinetics, and pregnancy rate. METHODS: Sixty infertile patients who underwent intracytoplasmic sperm injection (ICSI) cycles were included in the study. For each patient, CoQ10 level of the follicular fluid was measured by high-performance liquid chromatography system. After the ICSI of each oocyte, the relationship between the level of CoQ10 content of each follicular fluid, the subsequent embryo quality, and embryo morphokinetics was investigated...
February 9, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28174013/update-on-fertility-restoration-from-prepubertal-spermatogonial-stem-cells-how-far-are-we-from-clinical-practice
#16
Maria Grazia Giudice, Francesca de Michele, Jonathan Poels, Maxime Vermeulen, Christine Wyns
Fertility preservation in prepubertal boys facing gonadotoxic treatment is still at the experimental stage. Nevertheless cryopreservation of immature testicular tissue (ITT) obtained by small testicular biopsy is being increasingly proposed in reproductive care clinics for this purpose. Different approaches to in vivo or in vitro mature spermatogonial stem cells (SSCs) contained in ITT have been studied: autografting of testicular tissue pieces, transplantation of one's own purified germ cell suspensions, and in vitro maturation (IVM) for subsequent use of sperm for intra cytoplasmic sperm injection (ICSI)...
February 2, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28169188/clinical-outcomes-after-ivf-or-icsi-using-human-blastocysts-derived-from-oocytes-containing-aggregates-of-smooth-endoplasmic-reticulum
#17
Fumiaki Itoi, Yukiko Asano, Masashi Shimizu, Rika Nagai, Kanako Saitou, Hiroyuki Honnma, Yasutaka Murata
In this study the clinical and neo-natal outcomes after transfer of blastocysts derived from oocytes containing aggregates of smooth endoplasmic reticulum (SER) were compared between IVF and intracytoplasmic sperm injection (ICSI) cycles. Clinical and neo-natal outcomes of blastocysts in cycles with at least one SER metaphase II oocyte (SER + MII; SER + cycles) did not significantly differ between the two insemination methods. When SER + MII were cultured to day 5/6, fertilization, embryo cleavage and blastocyst rates were not significantly different between IVF and ICSI cycles...
January 25, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28168654/worldwide-prevalence-of-adverse-pregnancy-outcomes-associated-with-in-vitro-fertilization-intracytoplasmic-sperm-injection-among-multiple-births-a-systematic-review-and-meta-analysis-based-on-cohort-studies
#18
REVIEW
Jia-Bi Qin, Xiao-Qi Sheng, Hua Wang, Guo-Chong Chen, Jing Yang, Hong Yu, Tu-Bao Yang
PURPOSE: To perform a systematic review and meta-analysis of reported estimates of adverse pregnancy outcomes among multiple births conceived with in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI). METHODS: PubMed, Google Scholar, Cochrane Libraries and Chinese databases were searched through May 2016 for cohort studies assessing adverse pregnancy outcomes associated with IVF/ICSI multiple births. Random-effects meta-analyses were used to calculate pooled estimates of adverse pregnancy outcomes and, where appropriate, heterogeneity was explored in group-specific analyses...
March 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28162936/obstetric-and-neo-natal-outcomes-of-icsi-cycles-using-pentoxifylline-to-identify-viable-spermatozoa-in-patients-with-immotile-spermatozoa
#19
Purificación Navas, Alessio Paffoni, Giulia Intra, Antonio González-Utor, Ana Clavero, Maria Carmen Gonzalvo, Rocío Díaz, Rocío Peña, Liliana Restelli, Edgardo Somigliana, Enrico Papaleo, Jose A Castilla, Paola Viganò
Pentoxifylline (PF) represents an effective tool in stimulating motility and identifying viable spermatozoa in intracytoplasmic sperm injection (ICSI) patients presenting exclusively with immotile spermatozoa. However, its use is not universally accepted for its possible detrimental effects on oocytes, embryos or newborns. To evaluate whether PF use may affect obstetrical/neo-natal outcomes, 102 patients achieving a clinical pregnancy after a PF-ICSI in four IVF units in Spain and Italy were followed up after delivery...
January 24, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28157160/embryological-results-of-couples-undergoing-icsi-et-treatments-with-males-carrying-the-single-nucleotide-polymorphism-rs175080-of-the-mlh3-gene
#20
George Anifandis, Ourania Markandona, Konstantinos Dafopoulos, Christina Messini, Aspasia Tsezou, Marina Dimitraki, Panagiotis Georgoulias, Alexandros Daponte, Ioannis Messinis
Human MLH3 (hMLH3) gene has been suggested to play a role in the DNA mismatch repair mechanism, while it may also be associated with abnormal spermatogenesis and subsequently male infertility. The aim of the present study was to investigate possible relationships between the single nucleotide polymorphism (SNP) rs175080 in the MLH3 gene of males and the embryological results in couples undergoing intracytoplasmatic sperm injection-embryo transfer (ICSI-ET) treatments. A total of 132 men volunteered for the study and gave written informed consent...
February 2, 2017: International Journal of Molecular Sciences
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