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phytophthora infestans

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https://www.readbyqxmd.com/read/29329405/solanaceous-exocyst-subunits-are-involved-in-immunity-to-diverse-plant-pathogens
#1
Yu Du, Elysa J R Overdijk, Jeroen A Berg, Francine Govers, Klaas Bouwmeester
The exocyst, a multiprotein complex consisting of eight subunits, plays an essential role in many biological processes by mediating secretion of post-Golgi-derived vesicles towards the plasma membrane. In recent years, roles for plant exocyst subunits in pathogen defence have been uncovered, largely based on studies in the model plant Arabidopsis. Only a few studies have been undertaken to assign the role of exocyst subunits in plant defence in other plants species, including crops. In this study, predicted protein sequences from exocyst subunits were retrieved by mining databases from the Solanaceous plants Nicotiana benthamiana, tomato, and potato...
January 10, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29327646/late-blight-resistance-screening-of-major-wild-swedish-solanum-species-s-dulcamara-s-nigrum-and-s-physalifolium
#2
Kibrom Abreha, Åsa Lankinen, Laura Masini, Sofia Hydbom, Erik Andreasson
To understand the contribution of wild Solanum species to the epidemiology of potato late blight in Sweden, we characterized the resistance of the three putative alternative hosts; Solanum physalifolium, S. nigrum, and S. dulcamara to Phytophthora infestans, the causal agent of late blight. The pathogen sporulated in all ten investigated S. physalifolium genotypes, suggesting susceptibility (S phenotype). Field-grown S. physalifolium was naturally infected but could regrow, though highly infected genotypes were smaller at the end of the season...
January 12, 2018: Phytopathology
https://www.readbyqxmd.com/read/29300771/demonstration-of-translation-elongation-factor-3-activity-from-a-non-fungal-species-phytophthora-infestans
#3
Maria K Mateyak, Justyna K Pupek, Alexandra E Garino, McCllelan C Knapp, Sarah F Colmer, Terri Goss Kinzy, Stephen Dunaway
In most eukaryotic organisms, translation elongation requires two highly conserved elongation factors eEF1A and eEF2. Fungal systems are unique in requiring a third factor, the eukaryotic Elongation Factor 3 (eEF3). For decades, eEF3, a ribosome-dependent ATPase, was considered "fungal-specific", however, recent bioinformatics analysis indicates it may be more widely distributed among other unicellular eukaryotes. In order to determine whether divergent eEF3-like proteins from other eukaryotic organisms can provide the essential functions of eEF3 in budding yeast, the eEF3-like proteins from Schizosaccharomyes pombe and an oomycete, Phytophthora infestans, were cloned and expressed in Saccharomyces cerevisiae...
2018: PloS One
https://www.readbyqxmd.com/read/29297963/matrix-approach-to-the-simultaneous-detection-of-multiple-potato-pathogens-by-real-time-pcr
#4
M M Nikitin, N V Statsyuk, P A Frantsuzov, V G Dzhavakhiya, A G Golikov
AIM: Create a method for highly sensitive, selective, rapid, and easy-to-use detection and identification of economically significant potato pathogens, including viruses, bacteria and oomycetes, be it single pathogen, or a range of various pathogens occurring simultaneously. METHODS AND RESULTS: Test-systems for real-time PCR, operating in the unified amplification regime, have been developed for Phytophthora infestans, Pectobacterium atrosepticum, Dickeya dianthicola, Dickeya solani, Ralstonia solanacearum, Pectobacterium carotovorum, Clavibacter michiganensis subsp...
January 3, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29237394/prediction-of-mirna-and-identification-of-their-relationship-network-related-to-late-blight-disease-of-potato
#5
Mohammad Samir Farooqi, Animesh Kumar, D C Mishra, Sanjeev Kumar, Anil Rai, K K Chaturvedi, S B Lal, Anu Sharma
Late blight, is one of the most serious disease in potato caused by Phytophthora infestans. In spite of adopting so many agricultural practices this disease is still a threat for potato. Recent studies showed that microRNA (miRNA), an important gene expression regulator, plays a very important role in host-pathogen interaction by silencing genes either by destructing or blocking of translation of mRNA. To date only few miRNA have been discovered which are related to late blight disease of potato during host pathogen interaction...
December 13, 2017: MicroRNA
https://www.readbyqxmd.com/read/29234827/transcriptome-signatures-of-tomato-leaf-induced-by-phytophthora-infestans-and-functional-identification-of-transcription-factor-spwrky3
#6
Jun Cui, Pinsan Xu, Jun Meng, Jingbin Li, Ning Jiang, Yushi Luan
SpWRKY3 was identified as a resistance gene to Phytophthora infestans from Solanum pimpinellifolium L3708 and its transgenic tomato showed a significant resistance to P. infestans. This finding reveals the potential application of SpWRKY3 in future molecular breeding. Transcription factors (TFs) play crucial roles in the plant response to various pathogens. In this present study, we used comparative transcriptome analysis of tomatoes inoculated with and without Phytophthora infestans to identify 1103 differentially expressed genes...
December 12, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29223339/microrna396a-5p-and-3p-induce-tomato-disease-susceptibility-by-suppressing-target-genes-and-upregulating-salicylic-acid
#7
Lei Chen, Jun Meng, Junmiao Zhai, Pinsan Xu, Yushi Luan
Plants have evolved a variety of mechanisms to perceive and resist the assault of pathogens. The biotrophs, necrotrophs and hemibiotrophs are types of plant pathogens that activate diverse salicylic acid (SA) and jasmonic acid (JA) signaling pathways. In this study we showed that the expressions of miR396a-5p and -3p in Solanum lycopersicum (S. lycopersicum) were both down-regulated after infection by hemibiotroph Phytophthora infestans (P. infestans) and necrotroph Botrytis cinerea (B. cinerea) infection. Overexpression of miR396a-5p and -3p in transgenic tomato enhanced the susceptibility of S...
December 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29209352/melatonin-attenuates-potato-late-blight-by-disrupting-cell-growth-stress-tolerance-fungicide-susceptibility-and-homeostasis-of-gene-expression-in-phytophthora-infestans
#8
Shumin Zhang, Xianzhe Zheng, Russel J Reiter, Shun Feng, Ying Wang, Sen Liu, Liang Jin, Zhengguo Li, Raju Datla, Maozhi Ren
Phytophthora infestans (P. infestans) is the causal agent of potato late blight, which caused the devastating Irish Potato Famine during 1845-1852. Until now, potato late blight is still the most serious threat to potato growth and has caused significant economic losses worldwide. Melatonin can induce plant innate immunity against pathogen infection, but the direct effects of melatonin on plant pathogens are poorly understood. In this study, we investigated the direct effects of melatonin on P. infestans. Exogenous melatonin significantly attenuated the potato late blight by inhibiting mycelial growth, changing cell ultrastructure, and reducing stress tolerance of P...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29202688/rethinking-the-evolution-of-eukaryotic-metabolism-novel-cellular-partitioning-of-enzymes-in-stramenopiles-links-serine-biosynthesis-to-glycolysis-in-mitochondria
#9
Melania Abrahamian, Meenakshi Kagda, Audrey M V Ah-Fong, Howard S Judelson
BACKGROUND: An important feature of eukaryotic evolution is metabolic compartmentalization, in which certain pathways are restricted to the cytosol or specific organelles. Glycolysis in eukaryotes is described as a cytosolic process. The universality of this canon has been challenged by recent genome data that suggest that some glycolytic enzymes made by stramenopiles bear mitochondrial targeting peptides. RESULTS: Mining of oomycete, diatom, and brown algal genomes indicates that stramenopiles encode two forms of enzymes for the second half of glycolysis, one with and the other without mitochondrial targeting peptides...
December 4, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/29173073/in-vivo-assessment-of-plant-extracts-for-control-of-plant-diseases-a-sesquiterpene-ketolactone-isolated-from-curcuma-zedoaria-suppresses-wheat-leaf-rust
#10
Jae Woo Han, Sang Hee Shim, Kyoung Soo Jang, Yong Ho Choi, Quang Le Dang, Hun Kim, Gyung Ja Choi
As an alternative to synthetic pesticides, natural materials such as plant extracts and microbes have been considered to control plant diseases. In this study, methanol extracts of 120 plants were explored for in vivo antifungal activity against Rhizoctonia solani, Botrytis cinerea, Phytophthora infestans, Puccinia triticina, and Blumeria graminis f. sp. hordei. Of the 120 plant extracts, eight plant extracts exhibited a disease control efficacy of more than 90% against at least one of five plant diseases. In particular, a methanol extract of Curcuma zedoaria rhizomes exhibited strong activity against wheat leaf rust caused by P...
November 27, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/29140163/hsp70s-enhance-a-phytophthora-infestans-effector-induced-cell-death-via-a-mapk-cascade-in-nicotiana-benthamiana
#11
Joohyun Lee, So Eui Lee, SooHyun Oh, Eunyoung Seo, Doil Choi
A destructive pathogen, Phytophthora infestans, secretes hundreds of effectors for successful survival in its host plants. The effectors modulate plant defense system at diverse cellular compartments to take an advantage of pathogen survivals. There were a few researches shown the mode-of-action of each effectors and their interacting proteins in plant cells. Here, we investigated the mode-of-action of a P. infestans effector, Pi23226, which induces cell death in Nicotiana benthamiana. To identify its host factors, we performed co-immunoprecipitation and liquid chromatography-mass spectrometry, and selected members of HSP70 as candidates...
November 15, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29090012/infection-of-a-tomato-cell-culture-by-phytophthora-infestans-a-versatile-tool-to-study-phytophthora-host-interactions
#12
Charikleia Schoina, Klaas Bouwmeester, Francine Govers
BACKGROUND: The oomycete Phytophthora infestans causes late blight on potato and tomato. Despite extensive research, the P. infestans-host interaction is still poorly understood. To find new ways to further unravel this interaction we established a new infection system using MsK8 tomato cells. These cells grow in suspension and can be maintained as a stable cell line that is representative for tomato. RESULTS: MsK8 cells can host several Phytophthora species pathogenic on tomato...
2017: Plant Methods
https://www.readbyqxmd.com/read/29051751/evaluation-of-different-pcr-based-assays-and-lamp-method-for-rapid-detection-of-phytophthora-infestans-by-targeting-the-ypt1-gene
#13
Mehran Khan, Benjin Li, Yue Jiang, Qiyong Weng, Qinghe Chen
Late blight, caused by the oomycete Phytophthora infestans, is one of the most devastating diseases affecting potato and tomato worldwide. Early diagnosis of the P. infestans pathogen causing late blight should be the top priority for addressing disease epidemics and management. In this study, we performed a loop-mediated isothermal amplification (LAMP) assay, conventional polymerase chain reaction (PCR), nested PCR, and real-time PCR to verify and compare the sensitivity and specificity of the reaction based on the Ypt1 (Ras-related protein) gene of P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29034626/-sequence-analysis-and-expression-patterns-of-histone-h2a-variants-in-phytophthora-infestans
#14
Xiaowen Wang, Liyun Guo
In eukaryotic cells, DNA is packaged inside the nucleus together with histones to form nucleosomes. Each histone molecule contains two of each core histone subunits H2A, H2B, H3 and H4. Among core histones, the H2A family is of great interest due to the high diversity of specialized variants. These variants have shown important role in critical cellular processes. Epigenetic mechanism in oomycetes is barely known. Phytophthora infestans is a severe pathogen and a model species in oomycetes. In this study, we studied the sequence and expression pattern of H2A variants of P...
November 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29017458/lifestyle-gene-gain-and-loss-and-transcriptional-remodeling-cause-divergence-in-the-transcriptomes-of-phytophthora-infestans-and-pythium-ultimum-during-potato-tuber-colonization
#15
Audrey M V Ah-Fong, Jolly Shrivastava, Howard S Judelson
BACKGROUND: How pathogen genomes evolve to support distinct lifestyles is not well-understood. The oomycete Phytophthora infestans, the potato blight agent, is a largely biotrophic pathogen that feeds from living host cells, which become necrotic only late in infection. The related oomycete Pythium ultimum grows saprophytically in soil and as a necrotroph in plants, causing massive tissue destruction. To learn what distinguishes their lifestyles, we compared their gene contents and expression patterns in media and a shared host, potato tuber...
October 10, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28990716/genome-wide-characterization-of-phytophthora-infestans-metabolism-a-systems-biology-approach
#16
Sander Y A Rodenburg, Michael F Seidl, Dick de Ridder, Francine Govers
Genome-scale metabolic models (GEMs) provide a functional view of the complex network of biochemical reactions in the living cell. Initially mainly applied to reconstruct the metabolism of model organisms, the availability of increasingly sophisticated reconstruction methods and more extensive biochemical databases now make it possible to reconstruct GEMs for less characterized organisms as well, and have the potential to unravel the metabolism in pathogen-host systems. Here we present a GEM for the oomycete plant pathogen Phytophthora infestans as a first step towards an integrative model with its host...
October 9, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28935716/proteomic-analysis-of-phytophthora-infestans-reveals-the-importance-of-cell-wall-proteins-in-pathogenicity
#17
Svante Resjö, Maja Brus, Ashfaq Ali, Harold J G Meijer, Marianne Sandin, Francine Govers, Fredrik Levander, Laura Grenville-Briggs, Erik Andreasson
The oomycete Phytophthora infestans is the most harmful pathogen of potato. It causes the disease late blight, which generates increased yearly costs of up to one billion euro in the EU alone and is difficult to control. We have performed a large-scale quantitative proteomics study of six P. infestans life stages with the aim to identify proteins that change in abundance during development, with a focus on preinfectious life stages. Over 10 000 peptides from 2061 proteins were analyzed. We identified several abundance profiles of proteins that were up- or downregulated in different combinations of life stages...
November 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28911735/antifungal-activity-of-sterols-and-dipsacus-saponins-isolated-from-dipsacus-asper-roots-against-phytopathogenic-fungi
#18
Nam Hee Choi, Ja Yeong Jang, Gyung Ja Choi, Yong Ho Choi, Kyoung Soo Jang, Van Thu Nguyen, Byung-Sun Min, Quang Le Dang, Jin-Cheol Kim
The in vivo antifungal activity of crude extracts of Dipsacus asper roots was evaluated against the phytopathogenic fungi Botrytis cinerea, Colletotrichum coccodes, Blumeria graminis f. sp. hordei, Magnaporthe grisea, Phytophthora infestans, Puccinia recondita and Rhizoctonia solani using a whole-plant assay method. Ethyl acetate and acetone extracts, at 1000μg/mL, suppressed the development of tomato gray mold (TGM) and tomato late blight (TLB) by 90%. Through bioassay-guided isolation, five antifungal substances were isolated from the D...
September 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28890716/mining-the-volatilomes-of-plant-associated-microbiota-for-new-biocontrol-solutions
#19
REVIEW
Aurélien Bailly, Laure Weisskopf
Microbial lifeforms associated with land plants represent a rich source for crop growth- and health-promoting microorganisms and biocontrol agents. Volatile organic compounds (VOCs) produced by the plant microbiota have been demonstrated to elicit plant defenses and inhibit the growth and development of numerous plant pathogens. Therefore, these molecules are prospective alternatives to synthetic pesticides and the determination of their bioactivities against plant threats could contribute to the development of control strategies for sustainable agriculture...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28888160/nicotiana-benthamiana-matrix-metalloprotease-1-nmmp1-gene-confers-disease-resistance-to-phytophthora-infestans-in-tobacco-and-potato-plants
#20
Jang Ho Ha, Hyun A Jang, Ki-Beom Moon, Kwang Hyun Baek, Gyung Ja Choi, Doil Choi, Hye Sun Cho, Suk Yun Kwon, Jae-Heung Jeon, Sang-Keun Oh, Hyun-Soon Kim
We previously isolated Nicotiana benthamiana matrix metalloprotease 1 (NMMP1) from tobacco leaves. The NMMP1 gene encodes a highly conserved, Zn-containing catalytic protease domain that functions as a factor in the plant's defense against bacterial pathogens. Expression of NMMP1 was strongly induced during interactions between tobacco and one of its pathogens, Phytophthora infestans. To elucidate the role of the NMMP1 in defense of N. benthamiana against fungal pathogens, we performed gain-of-function and loss-of-function studies...
September 1, 2017: Journal of Plant Physiology
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