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https://www.readbyqxmd.com/read/28214330/automated-microfluidic-sorting-of-mammalian-cells-labeled-with-magnetic-microparticles-for-those-that-efficiently-express-and-secrete-a-protein-of-interest
#1
Xuan Droz, Niamh Harraghy, Etienne Lançon, Valérie Le Fourn, David Calabrese, Thierry Colombet, Pascal Liechti, Amar Rida, Pierre-Alain Girod, Nicolas Mermod
We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after capture. Using clinically compatible materials and procedures, we show that this approach is able to discriminate between cells that truly secrete high amounts of a protein from those that just display it at high levels on their surface without properly releasing it...
February 18, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28214161/human-factors-related-to-time-dependent-infection-control-measures-scrub-the-hub-for-venous-catheters-and-feeding-tubes
#2
Lindsay Caspari, Elizabeth Epstein, Amy Blackman, Li Jin, David A Kaufman
BACKGROUND: The use of catheter hub decontamination protocols is a common practice to reduce central line-associated bloodstream infections. However, few data exist on the most effective disinfection procedure prior to hub access accounting for human factors and time-dependent practices in real time in the clinical setting. METHODS: An observational design with a multimodal intervention was used in this study in a neonatal intensive care unit. Direct observations on nurse compliance of scrub times with decontamination when accessing of venous catheter and feeding tube hubs were conducted during 3 phases: (1) baseline period prior to any interventions; (2) during an educational intervention phase; and (3) during a timer intervention period when using a timing device, either an actual timer or music button...
February 15, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28213935/effect-of-a-one-step-self-etch-adhesive-on-endogenous-dentin-matrix-metalloproteinases
#3
Fabianni M Apolonio, Annalisa Mazzoni, Valeria Angeloni, Polliana M C Scaffa, Spartaco Santi, Vicente de Paulo A Saboia, Franklin R Tay, David H Pashley, Lorenzo Breschi
Degradation of the hybrid layer created in dentin by dentin adhesives is caused by enzyme activities present within the dentin matrix that destroy unprotected collagen fibrils. The aim of the present study was to evaluate the effect of a one-step self-etch adhesive system on dentinal matrix metalloproteinases 2 and 4 (MMP-2 and MMP-9, respectively) using in situ zymography and an enzymatic activity assay. The null hypothesis tested was that there are no differences in the activities of dentinal MMPs before and after treatment with a one-step adhesive system...
February 17, 2017: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/28213367/antibiotic-prophylaxis-for-infective-endocarditis-a-systematic-review-and-meta-analysis
#4
Thomas J Cahill, James L Harrison, Paul Jewell, Igho Onakpoya, John B Chambers, Mark Dayer, Peter Lockhart, Nia Roberts, David Shanson, Martin Thornhill, Carl J Heneghan, Bernard D Prendergast
OBJECTIVE: The use of antibiotic prophylaxis (AP) for prevention of infective endocarditis (IE) is controversial. In recent years, guidelines to cardiologists and dentists have advised restriction of AP to high-risk groups (in Europe and the USA) or against its use at all (in the UK). The objective of this systematic review was to appraise the evidence for use of AP for prevention of bacteraemia or IE in patients undergoing dental procedures. METHODS: We conducted electronic searches in Medline, Embase, Cochrane Library and ISI Web of Science...
February 17, 2017: Heart: Official Journal of the British Cardiac Society
https://www.readbyqxmd.com/read/28213276/engineering-approaches-in-sirna-delivery
#5
Anna Angela Barba, Sara Cascone, Diego Caccavo, Gaetano Lamberti, Gianluca Chiarappa, Michela Abrami, Gabriele Grassi, Mario Grassi, Giovanna Tomaiuolo, Stefano Guido, Valerio Brucato, Francesco Carfì Pavia, Giulio Ghersi, Vincenzo La Carrubba, Roberto Andrea Abbiati, Davide Manca
siRNAs are very potent drug molecules, able to silence genes involved in pathologies development. siRNAs have virtually an unlimited therapeutic potential, particularly for the treatment of inflammatory diseases. However, their use in clinical practice is limited because of their unfavorable properties to interact and not to degrade in physiological environments. In particular they are large macromolecules, negatively charged, which undergo rapid degradation by plasmatic enzymes, are subject to fast renal clearance/hepatic sequestration, and can hardly cross cellular membranes...
February 14, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28212868/vaginoplasty-with-autologous-buccal-mucosa-fenestrated-graft-in-two-patients-with-vaginal-agenesis-a-multidisciplinary-approach-and-literature-review
#6
Jessica L Chan, Pamela J Levin, Brian P Ford, David C Stanton, Samantha M Pfeifer
The objective of this case series is to describe the procedure and outcomes of a multidisciplinary approach to vaginoplasty using autologous buccal mucosa fenestrated grafts in two patients with vaginal agenesis. This procedure resulted in anatomic success with a functional neovagina with good vaginal length and caliber and satisfactory sexual function capacity and well healed buccal mucosa. There were no complications and patients were satisfied with surgical results. We conclude that the use of a single fenestrated graft of autologous buccal mucosa is a simple, effective procedure for the treatment of vaginal agenesis that results in an optimally functioning neovagina with respect to vaginal length, caliber, and sexual capacity...
February 14, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28211607/physiological-motion-modeling-for-organ-mounted-robots
#7
Nathan A Wood, David Schwartzman, Marco A Zenati, Cameron N Riviere
BACKGROUND: Organ-mounted robots passively compensate heartbeat and respiratory motion. In model-guided procedures, this motion can be a significant source of information that can be used to aid in localization or to add dynamic information to static preoperative maps. METHODS: Models for estimating periodic motion are proposed for both position and orientation. These models are then tested on animal data and optimal orders are identified. Finally, methods for online identification are demonstrated...
February 17, 2017: International Journal of Medical Robotics + Computer Assisted Surgery: MRCAS
https://www.readbyqxmd.com/read/28211198/safety-of-antifibrinolytics-in-cranial-vault-reconstructive-surgery-a-report-from-the-pediatric-craniofacial-collaborative-group
#8
Susan M Goobie, Franklyn P Cladis, Chris D Glover, Henry Huang, Srijaya K Reddy, Allison M Fernandez, David Zurakowski, Paul A Stricker
BACKGROUND: Antifibrinolytic therapy significantly decreases blood loss and transfusion in pediatric cranial vault reconstructive surgery; however, concern regarding the side effects profile limits clinical use. AIMS: The aim was to utilize the Pediatric Craniofacial Surgery Perioperative Registry database to identify the safety profile of antifibrinolytic therapy for cranial vault reconstructive surgery by reporting the incidence of adverse events as they relate to exposure to tranexamic acid and aminocaproic acid compared to no exposure to antifibrinolytics...
February 17, 2017: Paediatric Anaesthesia
https://www.readbyqxmd.com/read/28210988/real-life-experience-with-the-specific-reversal-agent-idarucizumab-for-the-management-of-emergency-situations-in-dabigatran-treated-patients-a-series-of-11-cases
#9
Milan R Vosko, Christof Bocksrucker, Rafał Drwiła, Petr Dulíček, Tomas Hauer, Johannes Mutzenbach, Christoph J Schlimp, David Špinler, Thomas Wolf, Daša Zugwitz
Non-vitamin K antagonist oral anticoagulants (NOACs) have a favorable benefit-risk profile compared with vitamin K antagonists. However, the lack of specific reversal agents has made the management of some patients receiving long-term treatment with NOACs problematic in emergency situations such as major bleeding events or urgent procedures. Idarucizumab, a fully humanized Fab antibody fragment that binds specifically and with high affinity to dabigatran, was recently approved for use in adult patients treated with dabigatran when rapid reversal of its anticoagulant effect is required...
February 16, 2017: Journal of Thrombosis and Thrombolysis
https://www.readbyqxmd.com/read/28209417/influence-of-hospital-level-practice-patterns-on-variation-in-the-application-of-minimally-invasive-surgery-in-united-states-pediatric-patients
#10
Arianne T Train, Carroll M Harmon, David H Rothstein
BACKGROUND: Although disparities in access to minimally invasive surgery are thought to exist in pediatric surgical patients in the United States, hospital-level practice patterns have not been evaluated as a possible contributing factor. METHODS: Retrospective cohort study using the Kids' Inpatient Database, 2012. Odds ratios of undergoing a minimally invasive compared to open operation were calculated for six typical pediatric surgical operations after adjustment for multiple patient demographic and hospital-level variables...
February 2, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28209345/multiobjective-optimization-of-cartilage-stress-for-non-invasive-patient-specific-recommendations-of-high-tibial-osteotomy-correction-angle-a-novel-method-to-investigate-alignment-correction
#11
Keke Zheng, Corey J Scholes, Junning Chen, David Parker, Qing Li
Medial opening wedge high tibial osteotomy (MOWHTO) is a surgical procedure to treat knee osteoarthritis associated with varus deformity. However, the ideal final alignment of the Hip-Knee-Ankle (HKA) angle in the frontal plane, that maximizes procedural success and post-operative knee function, remains controversial. Therefore, the purpose of this study was to introduce a subject-specific modeling procedure in determining the biomechanical effects of MOWHTO alignment on tibiofemoral cartilage stress distribution...
February 13, 2017: Medical Engineering & Physics
https://www.readbyqxmd.com/read/28207861/in-vitro-anti-herpes-simplex-virus-2-activity-of-salvia-desoleana-atzei-v-picci-essential-oil
#12
Valeria Cagno, Barbara Sgorbini, Cinzia Sanna, Cecilia Cagliero, Mauro Ballero, Andrea Civra, Manuela Donalisio, Carlo Bicchi, David Lembo, Patrizia Rubiolo
Salvia desoleana Atzei & V. Picci is an indigenous species in Sardinia island used in folk medicine to treat menstrual, digestive and central nervous system diseases. Nowadays, it is widely cultivated for the pleasant smell of its essential oil (EO), whose antimicrobial and antifungal activities have already been screened. This study evaluated the in vitro anti-Herpes Simplex Virus-2 (HSV-2) activity of S. desoleana EO, fractions and main components: linalyl acetate, alpha terpinyl acetate, and germacrene D...
2017: PloS One
https://www.readbyqxmd.com/read/28207317/effects-of-sensor-position-on-kinematic-data-obtained-with-an-inertial-sensor-system-during-gait-analysis-of-trotting-horses
#13
Valerie J Moorman, David D Frisbie, Christopher E Kawcak, C Wayne McIlwraith
OBJECTIVE To determine the effects of altering location of right forelimb and pelvic sensors on kinematic data obtained with a commonly used inertial sensor system during gait analysis of trotting horses. DESIGN Experimental study. ANIMALS 12 horses with mild to moderate lameness of at least 1 hind limb, with or without lameness of the forelimbs. PROCEDURES All horses were examined while trotting on a high-speed treadmill. The right forelimb sensor was tested at 3 anatomic locations in random order: dorsal midline and 2 cm medial and lateral to that midline...
March 1, 2017: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/28206782/process-dissociation-analyses-of-memory-changes-in-healthy-aging-preclinical-and-very-mild-alzheimer-disease-evidence-for-isolated-recollection-deficits
#14
Peter R Millar, David A Balota, Geoffrey B Maddox, Janet M Duchek, Andrew J Aschenbrenner, Anne M Fagan, Tammie L S Benzinger, John C Morris
OBJECTIVE: Recollection and familiarity are independent processes that contribute to memory performance. Recollection is dependent on attentional control, which has been shown to be disrupted in early stage Alzheimer's disease (AD), whereas familiarity is independent of attention. The present longitudinal study examines the sensitivity of recollection estimates based on Jacoby's (1991) process dissociation procedure to AD-related biomarkers in a large sample of well-characterized cognitively normal middle-aged and older adults (N = 519) and the extent to which recollection discriminates these individuals from individuals with very mild symptomatic AD (N = 64)...
February 16, 2017: Neuropsychology
https://www.readbyqxmd.com/read/28205574/neuropathic-pain
#15
REVIEW
Luana Colloca, Taylor Ludman, Didier Bouhassira, Ralf Baron, Anthony H Dickenson, David Yarnitsky, Roy Freeman, Andrea Truini, Nadine Attal, Nanna B Finnerup, Christopher Eccleston, Eija Kalso, David L Bennett, Robert H Dworkin, Srinivasa N Raja
Neuropathic pain is caused by a lesion or disease of the somatosensory system, including peripheral fibres (Aβ, Aδ and C fibres) and central neurons, and affects 7-10% of the general population. Multiple causes of neuropathic pain have been described and its incidence is likely to increase owing to the ageing global population, increased incidence of diabetes mellitus and improved survival from cancer after chemotherapy. Indeed, imbalances between excitatory and inhibitory somatosensory signalling, alterations in ion channels and variability in the way that pain messages are modulated in the central nervous system all have been implicated in neuropathic pain...
February 16, 2017: Nature Reviews. Disease Primers
https://www.readbyqxmd.com/read/28205208/skin-to-skin-care-for-procedural-pain-in-neonates
#16
REVIEW
Celeste Johnston, Marsha Campbell-Yeo, Timothy Disher, Britney Benoit, Ananda Fernandes, David Streiner, Darlene Inglis, Rebekah Zee
BACKGROUND: Skin-to-skin care (SSC), often referred to as 'kangaroo care' (KC) due to its similarity with marsupial behaviour of ventral maternal-infant contact, is one non-pharmacological intervention for pain control in infants. OBJECTIVES: The primary objectives were to determine the effect of SSC alone on pain from medical or nursing procedures in neonates compared to no intervention, sucrose or other analgesics, or additions to simple SSC such as rocking; and to determine the effects of the amount of SSC (duration in minutes), method of administration (e...
February 16, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28204935/a-review-of-body-dysmorphic-disorder-in-aesthetic-surgery-patients-and-the-legal-implications
#17
Iliana E Sweis, Jamie Spitz, David R Barry, Mimis Cohen
BACKGROUND: Body dysmorphic disorder (BDD) is an often under-recognized yet severe psychiatric illness. There is limited guidance for plastic surgeons in the USA in how to recognize and manage patients with BDD and protect themselves from potential litigation and harm. Therefore, in collaboration with legal counsel, we remind our profession of the serious nature of patients with BDD, provide warning signs for recognizing BDD, and critically evaluate the validity of informed consent and the legal ramifications of operating on such patients in the USA...
February 15, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28203316/causal-discovery-from-subsampled-time-series-data-by-constraint-optimization
#18
Antti Hyttinen, Sergey Plis, Matti Järvisalo, Frederick Eberhardt, David Danks
This paper focuses on causal structure estimation from time series data in which measurements are obtained at a coarser timescale than the causal timescale of the underlying system. Previous work has shown that such subsampling can lead to significant errors about the system's causal structure if not properly taken into account. In this paper, we first consider the search for the system timescale causal structures that correspond to a given measurement timescale structure. We provide a constraint satisfaction procedure whose computational performance is several orders of magnitude better than previous approaches...
August 2016: JMLR Workshop and Conference Proceedings
https://www.readbyqxmd.com/read/28202569/local-vs-volume-conductance-activity-of-field-potentials-in-the-human-subthalamic-nucleus
#19
Odeya Marmor, Dan Valsky, Mati Joshua, Atira S Bick, David Arkadir, Idit Tamir, Hagai Bergmann, Zvi Israel, Renana Eitan
BACKGROUND: Subthalamic nucleus field potentials have attracted growing research and clinical interest over the last few decades. However, it is unclear whether subthalamic field potentials represent locally generated neuronal subthreshold activity or volume conductance of the organized neuronal activity generated in the cortex. OBJECTIVE: This study aimed at understanding of the physiological origin of subthalamic field potentials and determining the most accurate method for recording them...
February 15, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28199884/three-dimensional-growth-of-human-endothelial-cells-in-an-automated-cell-culture-experiment-container-during-the-spacex-crs-8-iss-space-mission-the-spheroids-project
#20
Jessica Pietsch, Samuel Gass, Stefano Nebuloni, David Echegoyen, Stefan Riwaldt, Christin Baake, Johann Bauer, Thomas J Corydon, Marcel Egli, Manfred Infanger, Daniela Grimm
Human endothelial cells (ECs) were sent to the International Space Station (ISS) to determine the impact of microgravity on the formation of three-dimensional structures. For this project, an automatic experiment unit (EU) was designed allowing cell culture in space. In order to enable a safe cell culture, cell nourishment and fixation after a pre-programmed timeframe, the materials used for construction of the EUs were tested in regard to their biocompatibility. These tests revealed a high biocompatibility for all parts of the EUs, which were in contact with the cells or the medium used...
February 7, 2017: Biomaterials
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