keyword
MENU ▼
Read by QxMD icon Read
search

WGA

keyword
https://www.readbyqxmd.com/read/29149439/trends-in-utilization-of-outpatient-respiratory-syncytial-virus-prophylaxis-with-palivizumab-among-medicaid-and-commercially-insured-infants
#1
Melissa Pavilack, Robert A Clifford, Tara Gonzales, Amanda M Kong, Sally Wade, Kimmie K McLaurin
INTRODUCTION: Palivizumab is indicated for the prevention of respiratory syncytial virus (RSV) infection in high-risk children. Previous palivizumab utilization studies examined prior authorization claims but did not examine utilization within insured populations as a whole. This study describes outpatient palivizumab utilization trends and characterizes high-risk infants receiving palivizumab within Medicaid- and commercially insured populations. METHODS: Infants born July 1, 2003 through June 30, 2013 were identified in the MarketScan(®) Multistate Medicaid and Commercial claims databases...
November 17, 2017: Infectious Diseases and Therapy
https://www.readbyqxmd.com/read/29063991/mitochondrial-dna-content-is-associated-with-ploidy-status-maternal-age-and-oocyte-maturation-methods-in-mouse-blastocysts
#2
Xin Tao, Jessica N Landis, Rebecca L Krisher, Francesca E Duncan, Elena Silva, Agnieszka Lonczak, Richard T Scott, Yiping Zhan, Tinchun Chu, Richard T Scott, Nathan R Treff
PURPOSE: It was reported that mitochondrial DNA (mtDNA) was significantly increased in aneuploid human embryos compared to euploid embryos and was also associated with maternal age. In this study, we further established the mouse model of mtDNA quantitation in reproductive samples based on whole-genome amplification (WGA) and next-generation sequencing (NGS). METHODS: WGA followed by NGS-based mtDNA quantitation was first performed on 6 single- and 100-cell samples from a tumor-derived mouse cell line, which was exposed to ethidium bromide to reduce mtDNA content...
October 24, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29052233/heterogeneous-expression-of-glycoconjugates-in-the-primary-olfactory-centre-of-the-japanese-sword-tailed-newt-cynops-ensicauda
#3
T Matsui, K Tanaka, Y Kobayashi
Histochemical organization of the Caudata olfactory system remains largely unknown, despite this amphibian order showing phylogenetic diversity in the development of the vomeronasal organ and its primary centre, the accessory olfactory bulb. Here, we investigated the glycoconjugate distribution in the olfactory bulb of a semi-aquatic salamander, the Japanese sword-tailed newt (Cynops ensicauda), by histochemical analysis of the lectins that were present. Eleven lectins showed a specific binding to the olfactory and vomeronasal nerves as well as to the olfactory glomeruli...
October 19, 2017: Anatomia, Histologia, Embryologia
https://www.readbyqxmd.com/read/29045620/determination-and-pharmacokinetics-of-wga-in-rat-plasma-by-lc-ms-after-oral-administration-of-xanthoceras-sorbifolia-bunge-extract
#4
Junhong Ling, Nan Wang, Shaoning Wang, Yingchun Liu, Dandan Xia, Kaishun Bi
3-O-(3-O-angeloyl-6-O-β-D-glucopyranosyl)-β-D-glucopyranosyl-28-O-(2-α-L-rhamnopyranosyl-6-O-β-D-glucopyranosyl)-β-D-glucopyranosyl-16-deoxybarringtogenol A (WGA) is a potential anti-AD (Alzheimer's disease) active compound isolated from the husks of Xanthoceras sorbifolia Bunge. A rapid and accurate high-performance liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the quantification of WGA in rat plasma. Digoxin was used as internal standard (IS). Sample preparation was performed by liquid-liquid extraction using ethyl acetate-isopropanol (1:1, v/v)...
October 17, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/29041909/cost-utility-analysis-of-palivizumab-for-respiratory-syncytial-virus-infection-prophylaxis-in-preterm-infants-update-based-on-the-clinical-evidence-in-spain
#5
M Sanchez-Luna, R Burgos-Pol, I Oyagüez, J Figueras-Aloy, M Sánchez-Solís, F Martinón-Torres, X Carbonell-Estrany
BACKGROUND: This study aimed at estimating the efficiency of palivizumab in the prevention of Respiratory Syncytial Virus (RSV) infection and its sequelae in preterm infants (32(day 1)-35(day 0)weeks of gestational age -wGA-) in Spain. METHODS: A decision-tree model was developed to compare health benefits (Quality Adjusted Life Years-QALYs) and costs of palivizumab versus a non-prophylaxis strategy over 6 years. A hypothetical cohort of 1,000 preterm infants, 32(day 1)-35(day 0) wGA (4...
October 17, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29032163/massively-parallel-sequencing-on-human-cleavage-stage-embryos-to-detect-chromosomal-abnormality
#6
Zhi Zhou, Yanlin Ma, Qi Li, Yu Zhang, Yuanhua Huang, Zhihua Tu, Ning Ma, Minghong Li, Jun Wang, Jian Li, Weiying Lu
PURPOSE: Next-generation sequencing technology like MPS has recently been introduced to perform comprehensive chromosome screening on human trophectoderm samples for preimplantation embryo assessment. However, the potential of MPS in chromosome analysis of single cell from blastomeres has not yet been investigated. METHODS: In this study, 12 couples underwent MPS analysis, including 9 balanced translocation carriers and 3 carriers of numerical chromosomal abnormalities...
October 12, 2017: European Journal of Medical Genetics
https://www.readbyqxmd.com/read/29030178/a-lectin-mediated-delivery-system-for-the-intravesical-treatment-of-bladder-diseases-using-poly-l-glutamic-acid-as-polymeric-backbone
#7
Christina Apfelthaler, Patrick Gassenbauer, Sandra Weisse, Franz Gabor, Michael Wirth
In this study, we present a targeted drug delivery system to improve intravesical therapy of bladder diseases. The drug delivery system consists of wheat germ agglutinin (WGA) to facilitate specific interaction with the surface of bladder cells and α-poly-(L)-glutamic acid (PGA) as polymeric backbone to increase the number of drug molecules per targeting moiety. Additionally, fluorescein cadaverine was coupled to PGA to visualise and track the delivery system. Using 5637 single cells and cell monolayers, the optimised F-PGA-WGA delivery system, with an approximate molecular weight of 670kDa, could convince with its promising cytoadhesive as well as cytoinvasive potential...
October 13, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29029805/glycan-diversity-in-the-vomeronasal-organ-of-the-korean-roe-deer-capreolus-pygargus-a-lectin-histochemical-study
#8
Taekyun Shin, Jeongtae Kim, Yuna Choi, Meejung Ahn
Glycans in the epithelium play an important role in cell-to-cell communication and adhesion. No detailed evaluation of glycoconjugates in the vomeronasal organs (VNO) of the roe deer has been published previously. The aim of this study was to characterize glycan epitopes in the vomeronasal sensory epithelium (VSE) and non-sensory epithelium (VNSE) using lectin histochemistry. Glycan epitopes identified by lectin histochemistry were grouped as follows: N-acetylglucosamine (s-WGA, WGA, BSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose (RCA120, BSL-I, Jacalin, PNA, ECL), N-acetylgalactosamine (VVA, DBA, SBA, and SJA), fucose (UEA-I) and complex type N-glycan (PHA-E and PHA-L) groups...
October 10, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28993238/thalamo-insular-pathway-conveying-orofacial-muscle-proprioception-in-the-rat
#9
Fumihiko Sato, Yume Uemura, Chiharu Kanno, Yumi Tsutsumi, Akiko Tomita, Ayaka Oka, Takafumi Kato, Katsuro Uchino, Jumpei Murakami, Tahsinul Haque, Yoshihisa Tachibana, Atsushi Yoshida
Little is known about how proprioceptive signals arising from muscles reach to higher brain regions such as the cerebral cortex. We have recently shown that a particular thalamic region, the caudo-ventromedial edge (VPMcvm) of ventral posteromedial thalamic nucleus (VPM), receives the proprioceptive signals from jaw-closing muscle spindles (JCMSs) in rats. In this study, we further addressed how the orofacial thalamic inputs from the JCMSs were transmitted from the thalamus (VPMcvm) to the cerebral cortex in rats...
December 4, 2017: Neuroscience
https://www.readbyqxmd.com/read/28990722/disruption-of-calcineurin-catalytic-subunit-cnaa-in-epichlo%C3%A3-festucae-induces-symbiotic-defects-and-intrahyphal-hyphae-formation
#10
Milena Mitic, Daniel Berry, Emma Brasell, Kimberly Green, Carolyn A Young, Sanjay Saikia, Jasna Rakonjac, Barry Scott
Calcineurin is a conserved calcium/calmodulin-dependent protein phosphatase, consisting of a catalytic subunit A and a regulatory subunit B, which is involved in calcium-dependent signalling and regulation of various important cellular processes. In this study we functionally characterised the catalytic subunit A (CnaA) of the endophytic fungus Epichloë festucae that forms a symbiotic association with the grass host Lolium perenne. We deleted the CnaA-encoding gene cnaA in E. festucae and examined its role in hyphal growth, cell wall integrity and symbiosis...
October 9, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28971834/differential-localisation-and-anabolic-responsiveness-of-mtor-complexes-in-human-skeletal-muscle-in-response-to-feeding-and-exercise
#11
Nathan Hodson, Chris McGlory, Sara Y Oikawa, Stewart Jeromson, Zhe Song, Markus A Ruegg, D Lee Hamilton, Stuart M Phillips, Andrew Philp
Mechanistic target of rapamycin (mTOR) resides as two complexes within skeletal muscle. mTOR complex 1 (mTORC1-Raptor positive) regulates skeletal muscle growth, whereas mTORC2 (Rictor positive) regulates insulin sensitivity. To examine the regulation of these complexes in human skeletal muscle, we utilised immunohistochemical analysis to study the localisation of mTOR complexes prior to and following protein-carbohydrate feeding (FED) and resistance exercise plus protein-carbohydrate feeding (EXFED) in unilateral exercise model...
September 27, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28963821/chairside-molecular-imaging-of-aberrant-glycosylation-in-subjects-with-suspicious-oral-lesions-using-fluorescently-labeled-wheat-germ-agglutinin
#12
John Baeten, Alexander Johnson, Sumsum Sunny, Amritha Suresh, Praveen Birur, K Uma, Deepak Kademani
BACKGROUND: Aberrant sialylation is accepted as a carcinogenic biomarker. In previous work, fluorescently labeled wheat germ agglutinin (WGA) distinguished between cancerous and normal oral biopsies. The purpose of this study was to investigate WGA-fluorescein isothiocyanate (FITC) as a point-of-care tool for detecting oral malignant and dysplastic lesions in vivo. METHODS: Subject recruitment was divided into two groups: (1) the clinically normal oral mucosa group; or (2) the presence of clinically suspicious oral lesion(s) group...
September 30, 2017: Head & Neck
https://www.readbyqxmd.com/read/28939893/characterization-of-the-antifungal-functions-of-a-wga-fc-igg2a-fusion-protein-binding-to-cell-wall-chitin-oligomers
#13
Susie Coutinho Liedke, Daniel Zamith Miranda, Kamilla Xavier Gomes, Jorge Luis S Gonçalves, Susana Frases, Joshua D Nosanchuk, Marcio L Rodrigues, Leonardo Nimrichter, José Mauro Peralta, Allan J Guimarães
The majority of therapeutic strategies for mycosis require the protracted administration of antifungals, which can result in significant toxicities and have unacceptable failure rates. Hence, there is an urgent need for the development of improved therapeutic approaches, and monoclonal antibody-based drugs are potentially a powerful alternative to standard antifungals. To develop a broad antibody-like reagent against mycosis, wheat germ agglutinin (WGA) was linked to the effector Fc region of murine IgG2a. The resultant WGA-Fc displayed high affinity to purified chitin and bound efficiently to fungal cell walls, co-localizing with chitin, in patterns ranging from circular (Histoplasma capsulatum) to punctate (Cryptococcus neoformans) to labeling at the bud sites (Candida albicans and Saccharomyces cerevisiae)...
September 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28934494/derivatives-of-bst-like-gss-polymerase-with-improved-processivity-and-inhibitor-tolerance
#14
Igor P Oscorbin, Ekaterina A Belousova, Ulyana A Boyarskikh, Aleksandr I Zakabunin, Evgeny A Khrapov, Maksim L Filipenko
At the moment, one of the actual trends in medical diagnostics is a development of methods for practical applications such as point-of-care testing, POCT or research tools, for example, whole genome amplification, WGA. All the techniques are based on using of specific DNA polymerases having strand displacement activity, high synthetic processivity, fidelity and, most significantly, tolerance to contaminants, appearing from analysed biological samples or collected under purification procedures. Here, we have designed a set of fusion enzymes based on catalytic domain of DNA polymerase I from Geobacillus sp...
September 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28919755/synthetic-high-density-lipoprotein-nanodisks-for-targeted-withalongolide-delivery-to-adrenocortical-carcinoma
#15
Rui Kuai, Chitra Subramanian, Peter T White, Barbara N Timmermann, James J Moon, Mark S Cohen, Anna Schwendeman
Adrenocortical carcinoma (ACC) is a rare endocrine malignancy and has a 5-year survival rate of <35%. ACC cells require cholesterol for steroid hormone production, and this requirement is met via expression on the cell surface of a high level of SRB1, responsible for the uptake of high-density lipoproteins (HDLs), which carry and transport cholesterol in vivo. Here, we describe how this natural lipid carrier function of SRB1 can be utilized to improve the tumor-targeted delivery of a novel natural product derivative - withalongolide A 4,19,27-triacetate (WGA-TA) - which has shown potent antitumor efficacy, but poor aqueous solubility...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28916708/architectural-delineation-and-molecular-identification-of-extracellular-matrix-in-ascidian-embryos-and-larvae
#16
Jiankai Wei, Guilin Wang, Xiang Li, Ping Ren, Haiyan Yu, Bo Dong
The extracellular matrix (ECM) not only provides essential physical scaffolding for cellular constituents but also initiates crucial biochemical and biomechanical cues that are required for tissue morphogenesis. In this study, we utilized wheat germ agglutinin (WGA) staining to characterize the ECM architecture in ascidian embryos and larvae. The results showed three distinct populations of ECM presenting in Ciona embryogenesis: the outer layer localized at the surface of embryo, an inner layer of notochord sheath and the apical ECM secreted by the notochord...
September 15, 2017: Biology Open
https://www.readbyqxmd.com/read/28915574/comparative-study-of-whole-genome-amplification-and-next-generation-sequencing-performance-of-single-cancer-cells
#17
Anna Babayan, Malik Alawi, Michael Gormley, Volkmar Müller, Harriet Wikman, Ryan P McMullin, Denis A Smirnov, Weimin Li, Maria Geffken, Klaus Pantel, Simon A Joosse
BACKGROUND: Whole genome amplification (WGA) is required for single cell genotyping. Effectiveness of currently available WGA technologies in combination with next generation sequencing (NGS) and material preservation is still elusive. RESULTS: In respect to the accuracy of SNP/mutation, indel, and copy number aberrations (CNA) calling, the HiSeq2000 platform outperformed IonProton in all aspects. Furthermore, more accurate SNP/mutation and indel calling was demonstrated using single tumor cells obtained from EDTA-collected blood in respect to CellSave-preserved blood, whereas CNA analysis in our study was not detectably affected by fixation...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28910311/hearing-impairment-in-premature-newborns-analysis-based-on-the-national-hearing-screening-database-in-poland
#18
Katarzyna Wroblewska-Seniuk, Grazyna Greczka, Piotr Dabrowski, Joanna Szyfter-Harris, Jan Mazela
OBJECTIVES: The incidence of sensorineural hearing loss is between 1 and 3 per 1000 in healthy neonates and 2-4 per 100 in high-risk infants. The national universal neonatal hearing screening carried out in Poland since 2002 enables selection of infants with suspicion and/or risk factors of hearing loss. In this study, we assessed the incidence and risk factors of hearing impairment in infants ≤33 weeks' gestational age (wga). METHODS: We analyzed the database of the Polish Universal Newborns Hearing Screening Program from 2010 to 2013...
2017: PloS One
https://www.readbyqxmd.com/read/28906588/surface-glycan-pattern-of-canine-equine-and-ovine-bone-marrow-derived-mesenchymal-stem-cells
#19
Salvatore Desantis, Gianluca Accogli, Antonio Crovace, Edda G Francioso, Alberto Maria Crovace
The use of bone marrow-derived mesenchymal stem cells (MSCs) for clinical and experimental studies is increasing, but full characterization of MSCs in veterinary species is hindered by the variability in species-specific cell surface marker expression and antibody cross reactivity. Recent studies demonstrated that the glycans in the glycocalyx of MSCs are promising candidates as cell biomarkers. In the present study, we analyzed the glycocalyx of canine MSCs (cMSCs), ovine MSCs (oMSCs), and equine MSCs (eMSCs) using a cell microarray procedure in which MSCs were spotted on microarray slides and incubated with a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin...
September 14, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28903902/single-cell-gene-variation-analysis-method-for-single-gland
#20
Yan Zhou, Chao-Jie Wang, Chun-Chao Zhu, Jiang-Rong Chen, Ming Cheng, Yu-Liang Deng, Yan Guo
Single-cell analysis of heterogeneity has become the cutting-edge technology for profound understandings of relationships between cell populations. At present, common methods used in single cellular genomic research are mainly microfluidic technologies (Fluidigm) or based on microwells, both requiring a uniform size of cells at the entrance. However, the size of cells in specific tissues can vary from type to type. To address this issue, we need to establish a method to identify genomic features of individual cells of different sizes...
August 20, 2017: Yi Chuan, Hereditas
keyword
keyword
3456
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"