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https://www.readbyqxmd.com/read/28422419/carbohydrate-analog-microarrays-for-identification-of-lectin-selective-ligands
#1
Injae Shin, Ji Young Hyun, Cheol Wan Park, Yanna Liu, Daeun Kwon, Seong-Hyun Park, Sungjin Park, Jaeyoung Pai
Fifty five mono- and disaccharide analogs were prepared and used for construction of microarrays to uncover lectin-selective ligands. The microarray study shows that two disaccharide analogs, 28' and 44', selectively bind to S. tuberosum lectin (STL) and wheat germ agglutinin (WGA), respectively. The results of cell studies also indicate that 28' and 44' selectively block binding of STL and WGA to mammalian cells unlike a natural ligand LacNAc that suppresses binding of both STL and WGA to cells.
April 19, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28408603/single-cell-whole-genome-analyses-by-linear-amplification-via-transposon-insertion-lianti
#2
Chongyi Chen, Dong Xing, Longzhi Tan, Heng Li, Guangyu Zhou, Lei Huang, X Sunney Xie
Single-cell genomics is important for biology and medicine. However, current whole-genome amplification (WGA) methods are limited by low accuracy of copy-number variation (CNV) detection and low amplification fidelity. Here we report an improved single-cell WGA method, Linear Amplification via Transposon Insertion (LIANTI), which outperforms existing methods, enabling micro-CNV detection with kilobase resolution. This allowed direct observation of stochastic firing of DNA replication origins, which differs from cell to cell...
April 14, 2017: Science
https://www.readbyqxmd.com/read/28398642/wga-alexa-conjugates-for-axonal-tracing
#3
Sabrina L Levy, Joshua J White, Elizabeth P Lackey, Lindsey Schwartz, Roy V Sillitoe
Anatomical labeling approaches are essential for understanding brain organization. Among these approaches are various methods of performing tract tracing. However, a major hurdle to overcome when marking neurons in vivo is visibility. Poor visibility makes it challenging to image a desired neuronal pathway so that it can be easily differentiated from a closely neighboring pathway. As a result, it becomes impossible to analyze individual projections or their connections. The tracer that is chosen for a given purpose has a major influence on the quality of the tracing...
April 10, 2017: Current Protocols in Neuroscience
https://www.readbyqxmd.com/read/28397141/differentiation-dependent-rearrangements-of-actin-filaments-and-microtubules-hinder-apical-endocytosis-in-urothelial-cells
#4
Larisa Tratnjek, Rok Romih, Mateja Erdani Kreft
During differentiation, superficial urothelial cells (UCs) of the urinary bladder form the apical surface, which is almost entirely covered by urothelial plaques containing densely packed uroplakin particles. These urothelial plaques are the main structural components of the blood-urine permeability barrier in the urinary bladder. We have shown previously that endocytosis from the apical plasma membrane decreases during urothelial cell differentiation. Here, we investigated the role of actin filament and microtubule rearrangements in apical endocytosis of differentiating UCs cells using hyperplastic and normoplastic porcine urothelial models...
April 10, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28361040/different-phenotypes-of-mature-biofilm-in-flavobacterium-psychrophilum-share-a-potential-for-virulence-that-differs-from-planktonic-state
#5
Héctor A Levipan, Ruben Avendaño-Herrera
Flavobacterium psychrophilum is the etiological agent of bacterial coldwater disease and the rainbow trout fry syndrome in salmonid aquaculture worldwide. However, there have been few studies into the capacity of F. psychrophilum to form biofilms and how these cellular accretions differ from planktonic cells or how they affect potential virulence. We evaluated the biofilm formation by three Chilean isolates of F. psychrophilum (LM-02-Fp, LM-06-Fp, and LM-13-Fp) and two non-Chilean strains (JIP02/86 and NCMB1947(T)), and compared biofilm and planktonic states to obtain insights into expression differences of virulence- and biofilm-related genes (VBRGs)...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28359935/a-large-increase-of-sour-taste-receptor-cells-in-skn-1-deficient-mice-does-not-alter-the-number-of-their-sour-taste-signal-transmitting-gustatory-neurons
#6
Naohiro Maeda, Masataka Narukawa, Yoshiro Ishimaru, Kurumi Yamamoto, Takumi Misaka, Keiko Abe
The connections between taste receptor cells (TRCs) and innervating gustatory neurons are formed in a mutually dependent manner during development. To investigate whether a change in the ratio of cell types that compose taste buds influences the number of innervating gustatory neurons, we analyzed the proportion of gustatory neurons that transmit sour taste signals in adult Skn-1a(-/-) mice in which the number of sour TRCs is greatly increased. We generated polycystic kidney disease 1 like 3-wheat germ agglutinin (pkd1l3-WGA)/Skn-1a(+/+) and pkd1l3-WGA/Skn-1a(-/-) mice by crossing Skn-1a(-/-) mice and pkd1l3-WGA transgenic mice, in which neural pathways of sour taste signals can be visualized...
March 28, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28356418/impact-of-contaminating-dna-in-whole-genome-amplification-kits-used-for-metagenomic-shotgun-sequencing-for-infection-diagnosis
#7
Matthew Thoendel, Patricio Jeraldo, Kerryl E Greenwood-Quaintance, Janet Yao, Nicholas Chia, Arlen D Hanssen, Matthew P Abdel, Robin Patel
Whole genome amplification (WGA) is a useful tool for amplification of very small quantities of DNA for many uses, including metagenomic shotgun sequencing for infection diagnosis. Depending on the application, background DNA from WGA kits can be problematic. Three WGA kits were tested for their utility in a metagenomics approach to identify pathogens in sonicate fluid comprised of biofilms and other materials dislodged from the surfaces of explanted prosthetic joints using sonication. The Illustra Genomiphi V2, Illustra Single Cell Genomiphi, and Qiagen REPLI-g Single Cell kits were tested on identical sonicate fluid samples...
March 29, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28344519/assessment-of-repli-g-multiple-displacement-whole-genome-amplification-wga-techniques-for-metagenomic-applications
#8
Sofia Ahsanuddin, Ebrahim Afshinnekoo, Jorge Gandara, Mustafa Hakyemezoğlu, Daniela Bezdan, Samuel Minot, Nick Greenfield, Christopher E Mason
Amplification of minute quantities of DNA is a fundamental challenge in low-biomass metagenomic and microbiome studies because of potential biases in coverage, guanine-cytosine (GC) content, and altered species abundances. Whole genome amplification (WGA), although widely used, is notorious for introducing artifact sequences, either by amplifying laboratory contaminants or by nonrandom amplification of a sample's DNA. In this study, we investigate the effect of REPLI-g multiple displacement amplification (MDA; Qiagen, Valencia, CA, USA) on sequencing data quality and species abundance detection in 8 paired metagenomic samples and 1 titrated, mixed control sample...
April 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28340305/the-clinical-application-of-ngs-based-snp-haplotyping-for-pgd-of-hb-h-disease
#9
Linjun Chen, Zhenyu Diao, Zhipeng Xu, Jianjun Zhou, Guijun Yan, Haixiang Sun
This study investigated the usefulness of next-generation sequencing (NGS)-based single nucleotide polymorphism (SNP) haplotyping for preimplantation genetic diagnosis (PGD) of hemoglobin H (Hb H) disease. Multiple displacement amplification (MDA) was used for whole genome amplification (WGA) of biopsied trophectoderm (TE) cells. Gap-PCR and NGS-based SNP haplotyping was used to distinguish the two genotypes of -α(3.7)/αα and -(SEA)/αα for PGD of Hb H disease. One out of the ten blastocysts (B11) was successfully diagnosed as genotype -α(3...
June 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28280340/wheat-germ-agglutinin-conjugated-liposomes-incorporated-with-cardiolipin-to-improve-neuronal-survival-in-alzheimer-s-disease-treatment
#10
Yung-Chih Kuo, Che-Yu Lin, Jay-Shake Li, Yung-I Lou
Curcumin (CRM) and nerve growth factor (NGF) were entrapped in liposomes (LIP) with surface wheat germ agglutinin (WGA) to downregulate the phosphorylation of kinases in Alzheimer's disease (AD) therapy. Cardiolipin (CL)-conjugated LIP carrying CRM (CRM-CL/LIP) and also carrying NGF (NGF-CL/LIP) were used with AD models of SK-N-MC cells and Wistar rats after an insult with β-amyloid peptide (Aβ). We found that CRM-CL/LIP inhibited the expression of phosphorylated p38 (p-p38), phosphorylated c-Jun N-terminal kinase (p-JNK), and p-tau protein at serine 202 and prevented neurodegeneration of SK-N-MC cells...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28277825/the-efficacy-of-wga-modified-daunorubicin-anti-resistant-liposomes-in-treatment-of-drug-resistant-mcf-7-breast-cancer
#11
Shuang Liu, Xiao-Li Song, Yan-Hong Wang, Xiao-Min Wang, Yao Xiao, Xin Wang, Lan Cheng, Xue-Tao Li
BACKGROUND: Breast cancer is the most common malignancy and remains a leading cause of cancer-related deaths in female. Chemotherapy failure of breast cancer is mainly associated with multidrug resistance of cancer cells. PURPOSE: The WGA modified daunorubicin anti-resistant liposomes were developed for circumventing the multidrug resistance and eliminating cancer cells. METHODS: WGA was modified on liposomal surface for increasing the intracellular uptake...
February 21, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28259144/sag-qc-quality-control-of-single-amplified-genome-information-by-subtracting-non-target-sequences-based-on-sequence-compositions
#12
Toru Maruyama, Tetsushi Mori, Keisuke Yamagishi, Haruko Takeyama
BACKGROUND: Whole genome amplification techniques have enabled the analysis of unexplored genomic information by sequencing of single-amplified genomes (SAGs). Whole genome amplification of single bacteria is currently challenging because contamination often occurs in experimental processes. Thus, to increase the confidence in the analyses of sequenced SAGs, bioinformatics approaches that identify and exclude non-target sequences from SAGs are required. Since currently reported approaches utilize sequence information in public databases, they have limitations when new strains are the targets of interest...
March 4, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28249214/lectin-magnetic-separation-lms-for-isolation-of-toxoplasma-gondii-oocysts-from-concentrated-water-samples-prior-to-detection-by-microscopy-or-qpcr
#13
Jemere Bekele Harito, Andrew T Campbell, Kristoffer Relling Tysnes, J P Dubey, Lucy J Robertson
Although standard methods for analyzing water samples for the protozoan parasites Cryptosporidium spp. and Giardia duodenalis are available and widely used, equivalent methods for analyzing water samples for Toxoplasma gondii oocysts are lacking. This is partly due to the lack of a readily available, reliable immunomagnetic separation technique (IMS). Here we investigated the use of lectin-magnetic separation (LMS) for isolating T. gondii oocysts from water sample concentrates, with subsequent detection by microscopy or molecular methods...
February 21, 2017: Water Research
https://www.readbyqxmd.com/read/28237713/poly-l-glutamic-acid-drug-delivery-system-for-the-intravesical-therapy-of-bladder-cancer-using-wga-as-targeting-moiety
#14
C Apfelthaler, M Anzengruber, F Gabor, M Wirth
In the management of bladder cancer, surgical resection of the tumour is usually followed by intravesical instillation of immunomodulatives and/or chemotherapeutics. The purpose of this local intravesical therapy is to eliminate residual malignant cells after surgical intervention. The main limitation of a localised adjuvant therapy is the insufficient concentration of the active pharmaceutical ingredient (API) in malignant cells due to the unique structure of the human urothelium making it an exclusively hard to overcome barrier in the human body...
February 22, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28215876/in-vitro-lectin-binding-to-the-outer-surface-of-spirocerca-lupi-at-different-life-stages
#15
I Aroch, I Arogeti, A Marcovics, Y Spiegel, E Lavy
Spirocerca lupi is the esophageal nematode of dogs. Early, transient eosinophilia occurs in experimentally infected dogs, but is absent in advanced cases, suggesting that the nematode evades the dog's immune system. Lectins are proteins or glycoproteins of plant or animal origin, binding different saccharides, with varying specificities and avidities, used to characterize surface haptens in plant and animal parasitic helminths. This study investigated the in vitro binding of six lectins (Concanavalin A [ConA], wheat germ agglutinin [WGA], peanut agglutinin [PNA], soybean agglutinin [SBA], Dolichus biflorus agglutinin [DBA] and Ulex earopaeus agglutinin I [UEA]) to the surface of S...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28207771/comparison-of-whole-genome-amplification-techniques-for-human-single-cell-exome-sequencing
#16
Erik Borgström, Marta Paterlini, Jeff E Mold, Jonas Frisen, Joakim Lundeberg
BACKGROUND: Whole genome amplification (WGA) is currently a prerequisite for single cell whole genome or exome sequencing. Depending on the method used the rate of artifact formation, allelic dropout and sequence coverage over the genome may differ significantly. RESULTS: The largest difference between the evaluated protocols was observed when analyzing the target coverage and read depth distribution. These differences also had impact on the downstream variant calling...
2017: PloS One
https://www.readbyqxmd.com/read/28166424/lectin-histochemistry-for-detecting-cadmium-induced-changes-in-the-glycosylation-pattern-of-rat-placenta
#17
M C Díaz, N V González, C N Zanuzzi, R Najle, C G Barbeito
Cadmium (Cd) is an industrial and environmental pollutant that produces toxic effects on gametogenesis, pre- and post-implantation embryos, and the placenta. Because the effects of acute Cd intoxication on the placenta are not well understood, we investigated changes in its glycosylated components in Cd treated dams at days 4, 7, 10 and 15 of gestation using lectin histochemistry. CdCl2 was administered to pregnant rats; control animals received sterile normal saline. Placentas were processed for DBA, Con A, SBA, PNA, UEA-I, RCA-I and WGA lectin histochemistry to evaluate changes in the carbohydrate pattern of the placenta that might modify cell interactions and contribute to embryonic alterations...
2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/28124793/fluoroacetamide-moieties-as-nmr-spectroscopy-probes-for-the-molecular-recognition-of-glcnac-containing-sugars-modulation-of-the-ch-%C3%AF-stacking-interactions-by-different-fluorination-patterns
#18
Luca Unione, Maria Alcalá, Begoña Echeverria, Sonia Serna, Ana Ardá, Antonio Franconetti, F Javier Cañada, Tammo Diercks, Niels Reichardt, Jesús Jiménez-Barbero
We herein propose the use of fluoroacetamide and difluoroacetamide moieties as sensitive tags for the detection of sugar-protein interactions by simple (1) H and/or (19) F NMR spectroscopy methods. In this process, we have chosen the binding of N,N'-diacetyl chitobiose, a ubiquitous disaccharide fragment in glycoproteins, by wheat-germ agglutinin (WGA), a model lectin. By using saturation-transfer difference (STD)-NMR spectroscopy, we experimentally demonstrate that, under solution conditions, the molecule that contained the CHF2 CONH- moiety is the stronger aromatic binder, followed by the analogue with the CH2 FCONH- group and the natural molecule (with the CH3 CONH- fragment)...
January 26, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28112458/plasticity-of-neonatal-neuronal-networks-in-very-premature-infants-source-localization-of-temporal-theta-activity-the-first-endogenous-neural-biomarker-in-temporoparietal-areas
#19
L Routier, M Mahmoudzadeh, M Panzani, H Azizollahi, S Goudjil, G Kongolo, F Wallois
Temporal theta slow-wave activity (TTA-SW) in premature infants is a specific signature of the early development of temporal networks, as it is observed at the turning point between non-sensory driven spontaneous local processing and cortical network functioning. The role in development and the precise location of TTA-SW remain unknown. Previous studies have demonstrated that preterms from 28 weeks of gestational age (wGA) are able to discriminate phonemes and voice, supporting the idea of a prior genetic structural or activity-dependent fingerprint that would prepare the auditory network to compute auditory information at the onset of thalamocortical connectivity...
January 23, 2017: Human Brain Mapping
https://www.readbyqxmd.com/read/28093222/swainsonine-induced-lysosomal-storage-disease-in-goats-caused-by-the-ingestion-of-sida-rodrigoi-monteiro-in-north-western-argentina
#20
Juan Francisco Micheloud, Raúl Marin, Luis Adrián Colque-Caro, Olga Gladys Martínez, Dale Gardner, Eduardo Juan Gimeno
There are numerous poisonous plants that can induce intralysosomal accumulation of glycoproteins and neurologic syndromes. Here we describe for the first time, a disease caused by ingesting Sida rodrigoi Monteiro in goats in North-western Argentina. The animals showed weight loss, indifference to the environment, unsteady gait and ataxia. Histopathologic studies showed vacuolization in cells of various organs, mainly in the CNS. The material deposited in the cells was positive for LCA (Lens culinaris agglutinin), WGA (Triticum vulgaris agglutinin), sWGA (succinyl-Triticum vulgaris agglutinin) and Con-A (Concanavalia ensiformis agglutinin) lectins...
January 16, 2017: Toxicon: Official Journal of the International Society on Toxinology
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