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https://www.readbyqxmd.com/read/29317261/insulin-induced-translocation-of-ir-to-the-nucleus-in-insulin-responsive-cells-requires-a-nuclear-translocation-sequence
#1
Dov Kesten, Miriam Horovitz-Fried, Tamar Brutman-Barazani, Sanford R Sampson
Insulin binding to its cell surface receptor (IR) activates a cascade of events leading to its biological effects. The Insulin-IR complex is rapidly internalized and then is either recycled back to the plasma membrane or sent to lysosomes for degradation. Although most of the receptor is is recycled or degraded, a small amount may escape this pathway and migrate to the nucleus of the cell where it might be important in promulgation of receptor signals. In this study we explored the mechanism by which insulin induces IR translocation to the cell nucleus...
January 6, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29279458/whole-genome-sequencing-revealed-armadillo-repeat-containing-5-armc5-mutation-in-a-chinese-family-with-acth-independent-macronodular-adrenal-hyperplasia
#2
Qian Zhang, Liang Cui, Jiang-Ping Gao, Wen-Hua Yan, Nan Jin, Kang Chen, Li Zang, Jin Du, Xian-Ling Wang, Qing-Hua Guo, Guo-Qing Yang, Li-Juan Yang, Jian-Ming Ba, Wei-Jun Gu, Zhao-Hui Lv, Jing-Tao Dou, Yi-Ming Mu, Ju-Ming Lu
Primary macronodular adrenal hyperplasia (PMAH), also known in the past as bilateral macronodular adrenalhyperplasia or adrenocorticotropin (ACTH)-independent macronodular adrenal hyperplasia, is a rare type of Cushing's syndrome (CS) and is associated with bilateralenlargement of the adrenal glands. It accounts for <1% of all endogenous cases of CS. In order toidentify the pathogenic mutations in the causative gene of (AIMAH pedigrees, Whole-genome sequencing of three patients in family I was used to retrieve candidate causative genes...
December 27, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/29276491/distribution-of-glycan-motifs-at-the-surface-of-midgut-cells-in-the-cotton-leafworm-spodoptera-littoralis-demonstrated-by-lectin-binding
#3
Tomasz Walski, Kristof De Schutter, Kaat Cappelle, Els J M Van Damme, Guy Smagghe
Glycans are involved in many biological phenomena, including signal transduction, cell adhesion, immune response or differentiation. Although a few papers have reported on the role of glycans in the development and proper functioning of the insect midgut, no data are available regarding the localization of the glycan structures on the surface of the cells in the gut of insects. In this paper, we analyzed the spatial distribution of glycans present on the surface of the midgut cells in larvae of the cotton leafworm Spodoptera littoralis, an important agricultural pest insect worldwide...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29248314/the-effect-of-rebamipide-ophthalmic-suspension-on-ocular-surface-mucins-in-soft-contact-lens-wearers
#4
Chika Shigeyasu, Masakazu Yamada, Yoko Akune, Masaki Fukui
PURPOSE: To evaluate the changes in ocular surface mucins with 2%rebamipide ophthalmic suspension treatment in soft contact lens (SCL) wearers. Rebamipide suspension is a mucin secretagogue approved for the treatment of dry eye syndrome in Japan. In this study, the fluorescence intensity of wheat germ agglutinin conjugate of fluorescein (F-WGA) was used as a marker of membrane-associated mucins, and sialic acid concentration in tear fluids as a marker of secreted mucins. METHODS: Thirty-two eyes of 16 SCL wearers with discomfort were treated with rebamipide suspension at a dose of one drop in each eye four times daily for two weeks...
December 13, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/29228925/daily-activity-patterns-influence-retinal-morphology-signatures-of-selection-and-spectral-tuning-of-opsin-genes-in-colubrid-snakes
#5
E Hauzman, D M O Bonci, E Y Suárez-Villota, M Neitz, D F Ventura
BACKGROUND: Morphological divergences of snake retinal structure point to complex evolutionary processes and adaptations. The Colubridae family has a remarkable variety of retinal structure that can range from all-cone and all-rod to duplex (cone/rod) retinas. To explore whether nocturnal versus diurnal activity is responsible for constraints on molecular evolution and plays a role in visual opsin spectral tuning of colubrids, we carried out molecular evolution analyses of the visual opsin genes LWS, RH1, and SWS1 from 17 species and performed morphological analyses...
December 11, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/29224066/genome-wide-copy-number-alteration-detection-in-preimplantation-genetic-diagnosis
#6
Lieselot Deleye, Dieter De Coninck, Dieter Deforce, Filip Van Nieuwerburgh
Shallow whole genome sequencing has recently been introduced for genome-wide detection of chromosomal copy number alterations (CNAs) in preimplantation genetic diagnosis (PGD), using only 4-7 trophectoderm cells biopsied from day-5 embryos. This chapter describes the complete method, starting from whole genome amplification (WGA) on isolated blastomere(s), up to data analysis for CNA detection. The process is described generically and can also be used to perform CNA analysis on a limited number of cells (down to a single cell) in other applications...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29215049/str-profiling-and-copy-number-variation-analysis-on-single-preserved-cells-using-current-whole-genome-amplification-methods
#7
Ann-Sophie Vander Plaetsen, Lieselot Deleye, Senne Cornelis, Laurentijn Tilleman, Filip Van Nieuwerburgh, Dieter Deforce
The growing interest in liquid biopsies for cancer research and cell-based non-invasive prenatal testing (NIPT) invigorates the need for improved single cell analysis. In these applications, target cells are extremely rare and fragile in peripheral circulation, which makes the genetic analysis very challenging. To overcome these challenges, cell stabilization and unbiased whole genome amplification are required. This study investigates the performance of four WGA methods on single or a limited number of cells after 24 hour of Streck Cell-Free DNA BCT preservation...
December 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29213128/complement-5a-receptor-deficiency-does-not-influence-adverse-cardiac-remodeling-after-pressure-overload-in-mice
#8
Judith J de Haan, Lena Bosch, Anouska Borgman, Marissa Bastemeijer, Maike A D Brans, Sander M van de Weg, Dominique P V de Kleijn, Joost P G Sluijter, Hamid El Azzouzi, Saskia C A de Jager
Hypertension is one of the most common risk factors for the development heart failure in the general population. Inflammation plays a central role in this adverse remodeling and eventually to the development of heart failure. Circulating levels of Complement factor 5a (C5a) are increased in hypertensive patients and the C5a receptor is associated with the presence of cardiac fibrosis and inflammation in an experimental hypertension model. To test if C5aR is involved in adverse cardiac remodeling following pressure-overload, we induced transverse aortic constriction (TAC) in wildtype and C5a receptor deficient mice (C5aR-/-)...
December 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29207637/wheat-germ-agglutinin-induced-paraptosis-like-cell-death-and-protective-autophagy-is-mediated-by-autophagy-linked-fyve-inhibition
#9
Tsung Lin Tsai, Hao Chen Wang, Chun Hua Hung, Peng Chan Lin, Yi San Lee, Helen H W Chen, Wu Chou Su
Wheat germ agglutinin (WGA) is a lectin that specifically binds cell surface glycoproteins and disrupts nuclear pore complex function through its interaction with POM121. Our data indicate WGA induces paraptosis-like cell death without caspase activation. We observed the main features of paraptosis, including cytoplasmic vacuolation, endoplasmic reticulum dilation and increased ER stress, and the unfolded protein response in WGA-treated cervical carcinoma cells. Conversion of microtubule-associated protein I light chain 3 (LC3-I) into LC3-II and punctuate formation suggestive of autophagy were observed in WGA-treated cells...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29206917/tunicamycin-induced-er-stress-in-breast-cancer-cells-neither-expresses-grp78-on-the-surface-nor-secretes-it-into-the-media
#10
Jesús E Serrano-Negrón, Zhenbo Zhang, Andrea P Rivera-Ruiz, Aditi Banerjee, Eva C Romero-Nutz, Neysharie Sánchez-Torres, Krishna Baksi, Dipak K Banerjee
GRP78 (an Mr 78 kDa calcium dependent glucose binding protein) located in ER lumen. It functions as ER chaperone and translocates proteins for glycosylation at the asparagine residue present in the sequon Asn-X-Ser/Thr. Paraffin sections from N-glycosylation inhibitor tunicamycin treated ER-/PR-/HER2+ (double negative) breast tumor in athymic nude mice exhibited reduced N-glycan but increased GRP78 expression. We have evaluated the effect of tunicamycin on cellular localization of GRP78 in metastatic human breast cancer cells MDA-MB-231 (ER-/PR-/HER2-)...
December 1, 2017: Glycobiology
https://www.readbyqxmd.com/read/29195833/lutein-levels-in-arterial-cord-blood-correlate-with-neuroprotein-activin-a-in-healthy-preterm-and-term-newborns-a-trophic-role-for-lutein
#11
Simonetta Picone, Alberto Ritieni, Adele Fabiano, Giulia Graziani, Piermichele Paolillo, Giovanni Livolti, Fabio Galvano, Diego Gazzolo
BACKGROUND: Lutein (LT) is a naturally occurring xanthophyll carotenoid most predominant in the central nervous system (CNS), but its neurotrophic role is still debated. We therefore investigated whether cord blood concentrations correlated with a well-established neurobiomarker, namely activin A. METHODS: We conducted a prospective study on the distribution of LT and activin A in arterial cord blood of healthy preterm (n=50) and term (n=82) newborns according to weeks of gestational age (wGA) and gender...
November 28, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/29176788/preservation-of-three-dimensional-spatial-structure-in-the-gut-microbiome
#12
Yuko Hasegawa, Jessica L Mark Welch, Blair J Rossetti, Gary G Borisy
Preservation of three-dimensional structure in the gut is necessary in order to analyze the spatial organization of the gut microbiota and gut luminal contents. In this study, we evaluated preparation methods for mouse gut with the goal of preserving micron-scale spatial structure while performing fluorescence imaging assays. Our evaluation of embedding methods showed that commonly used media such as Tissue-Tek Optimal Cutting Temperature (OCT) compound, paraffin, and polyester waxes resulted in redistribution of luminal contents...
2017: PloS One
https://www.readbyqxmd.com/read/29159968/histochemical-and-immunohistochemical-characterization-of-rodlet-cells-in-the-intestine-of-two-teleosts-anguilla-anguilla-and-cyprinus-carpio
#13
G Bosi, J A DePasquale, M Manera, G Castaldelli, L Giari, B Sayyaf Dezfuli
Rodlet cells (RC) are characterized by a distinctive cell cortex and conspicuous inclusions named "rodlets." These cells are particularly abundant and large in size in intestine of eels. Histochemical, immunohistochemical and ultrastructural investigations were carried out on European eel Anguilla anguilla and Common carp Cyprinus carpio from Northern Italy. Eight biotinylated lectins were used to probe for specific carbohydrate residues in deparaffinized, hydrated intestinal sections of eel and carp. Five antibodies were tested on intestinal sections of both fish species: inducible nitric oxide synthase (i-NOS), leu-enkephalin, lysozyme, serotonin and tumour necrosis factor-α...
November 21, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29156562/biodegradable-chitosan-decreases-the-immune-response-to-trichinella-spiralis-in-mice
#14
Klaudia Brodaczewska, Natalia Wolaniuk, Katarzyna Lewandowska, Katarzyna Donskow-Łysoniewska, Maria Doligalska
The purpose of this study was to evaluate the potential of chitosan units released during natural degradation of the polymer to activate the immune system against T. spiralis infection. High molecular weight chitosan was injected intraperitoneally into C57BL/6 mice. Flow cytometry and cytokine concentration, measured by ELISA, were used to characterize peritoneal cell populations during T. spiralis infection. The strong chemo-attractive properties of chitosan caused considerable infiltration into the peritoneal cavity of CD11b⁺ cells, with reduced expression of MHC class II, CD80, CD86, Dectin-1 or CD23 receptors in comparison to T...
November 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29155760/echocardiographic-and-histological-examination-of-cardiac-morphology-in-the-mouse
#15
Delphine Baudouy, Jean-François Michiels, Ana Vukolic, Kay-Dietrich Wagner, Nicole Wagner
An increasing number of genetically modified mouse models has become available in recent years. Moreover, the number of pharmacological studies performed in mice is high. Phenotypic characterization of these mouse models also requires the examination of cardiac function and morphology. Echocardiography and magnetic resonance imaging (MRI) are commonly used approaches to characterize cardiac function and morphology in mice. Echocardiographic and MRI equipment specialized for use in small rodents is expensive and requires a dedicated space...
October 26, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29149439/trends-in-utilization-of-outpatient-respiratory-syncytial-virus-prophylaxis-with-palivizumab-among-medicaid-and-commercially-insured-infants
#16
Melissa Pavilack, Robert A Clifford, Tara Gonzales, Amanda M Kong, Sally Wade, Kimmie K McLaurin
INTRODUCTION: Palivizumab is indicated for the prevention of respiratory syncytial virus (RSV) infection in high-risk children. Previous palivizumab utilization studies examined prior authorization claims but did not examine utilization within insured populations as a whole. This study describes outpatient palivizumab utilization trends and characterizes high-risk infants receiving palivizumab within Medicaid- and commercially insured populations. METHODS: Infants born July 1, 2003 through June 30, 2013 were identified in the MarketScan(®) Multistate Medicaid and Commercial claims databases...
November 17, 2017: Infectious Diseases and Therapy
https://www.readbyqxmd.com/read/29063991/mitochondrial-dna-content-is-associated-with-ploidy-status-maternal-age-and-oocyte-maturation-methods-in-mouse-blastocysts
#17
Xin Tao, Jessica N Landis, Rebecca L Krisher, Francesca E Duncan, Elena Silva, Agnieszka Lonczak, Richard T Scott, Yiping Zhan, Tinchun Chu, Richard T Scott, Nathan R Treff
PURPOSE: It was reported that mitochondrial DNA (mtDNA) was significantly increased in aneuploid human embryos compared to euploid embryos and was also associated with maternal age. In this study, we further established the mouse model of mtDNA quantitation in reproductive samples based on whole-genome amplification (WGA) and next-generation sequencing (NGS). METHODS: WGA followed by NGS-based mtDNA quantitation was first performed on 6 single- and 100-cell samples from a tumor-derived mouse cell line, which was exposed to ethidium bromide to reduce mtDNA content...
October 24, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29052233/heterogeneous-expression-of-glycoconjugates-in-the-primary-olfactory-centre-of-the-japanese-sword-tailed-newt-cynops-ensicauda
#18
T Matsui, K Tanaka, Y Kobayashi
Histochemical organization of the Caudata olfactory system remains largely unknown, despite this amphibian order showing phylogenetic diversity in the development of the vomeronasal organ and its primary centre, the accessory olfactory bulb. Here, we investigated the glycoconjugate distribution in the olfactory bulb of a semi-aquatic salamander, the Japanese sword-tailed newt (Cynops ensicauda), by histochemical analysis of the lectins that were present. Eleven lectins showed a specific binding to the olfactory and vomeronasal nerves as well as to the olfactory glomeruli...
October 19, 2017: Anatomia, Histologia, Embryologia
https://www.readbyqxmd.com/read/29045620/determination-and-pharmacokinetics-of-wga-in-rat-plasma-by-lc-ms-after-oral-administration-of-xanthoceras-sorbifolia-bunge-extract
#19
Junhong Ling, Nan Wang, Shaoning Wang, Yingchun Liu, Dandan Xia, Kaishun Bi
3-O-(3-O-angeloyl-6-O-β-D-glucopyranosyl)-β-D-glucopyranosyl-28-O-(2-α-L-rhamnopyranosyl-6-O-β-D-glucopyranosyl)-β-D-glucopyranosyl-16-deoxybarringtogenol A (WGA) is a potential anti-AD (Alzheimer's disease) active compound isolated from the husks of Xanthoceras sorbifolia Bunge. A rapid and accurate high-performance liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the quantification of WGA in rat plasma. Digoxin was used as internal standard (IS). Sample preparation was performed by liquid-liquid extraction using ethyl acetate-isopropanol (1:1, v/v)...
October 17, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/29041909/cost-utility-analysis-of-palivizumab-for-respiratory-syncytial-virus-infection-prophylaxis-in-preterm-infants-update-based-on-the-clinical-evidence-in-spain
#20
M Sanchez-Luna, R Burgos-Pol, I Oyagüez, J Figueras-Aloy, M Sánchez-Solís, F Martinón-Torres, X Carbonell-Estrany
BACKGROUND: This study aimed at estimating the efficiency of palivizumab in the prevention of Respiratory Syncytial Virus (RSV) infection and its sequelae in preterm infants (32(day 1)-35(day 0)weeks of gestational age -wGA-) in Spain. METHODS: A decision-tree model was developed to compare health benefits (Quality Adjusted Life Years-QALYs) and costs of palivizumab versus a non-prophylaxis strategy over 6 years. A hypothetical cohort of 1,000 preterm infants, 32(day 1)-35(day 0) wGA (4...
October 17, 2017: BMC Infectious Diseases
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