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Embryo biopsy

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https://www.readbyqxmd.com/read/28634372/dna-methylation-changes-in-endometrium-and-correlation-with-gene-expression-during-the-transition-from-pre-receptive-to-receptive-phase
#1
Viktorija Kukushkina, Vijayachitra Modhukur, Marina Suhorutšenko, Maire Peters, Reedik Mägi, Nilufer Rahmioglu, Agne Velthut-Meikas, Signe Altmäe, Francisco J Esteban, Jaak Vilo, Krina Zondervan, Andres Salumets, Triin Laisk-Podar
The inner uterine lining (endometrium) is a unique tissue going through remarkable changes each menstrual cycle. Endometrium has its characteristic DNA methylation profile, although not much is known about the endometrial methylome changes throughout the menstrual cycle. The impact of methylome changes on gene expression and thereby on the function of the tissue, including establishing receptivity to implanting embryo, is also unclear. Therefore, this study used genome-wide technologies to characterize the methylome and the correlation between DNA methylation and gene expression in endometrial biopsies collected from 17 healthy fertile-aged women from pre-receptive and receptive phase within one menstrual cycle...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28633287/genetic-diseases-and-aneuploidies-can-be-detected-with-a-single-blastocyst-biopsy-a-successful-clinical-approach
#2
Maria Giulia Minasi, Francesco Fiorentino, Alessandra Ruberti, Anil Biricik, Elisabetta Cursio, Ettore Cotroneo, Maria Teresa Varricchio, Matteo Surdo, Francesca Spinella, Ermanno Greco
STUDY QUESTION: Can simultaneous detection of aneuploidies and genetic diseases or chromosomal aberrations in blastocysts reduce the chance of transferring embryos with low implantation potential, guaranteeing good clinical outcomes? SUMMARY ANSWER: The screening for chromosomal aneuploidies revealed that 50.6% of blastocysts diagnosed free of genetic disease or balanced, were aneuploid, therefore avoiding the transfer of blastocysts potentially resulting in implantation failures, miscarriages, or in some cases, in health affected live births...
June 12, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28614992/preimplantation-genetic-screening
#3
Joyce C Harper
Preimplantation genetic diagnosis was first successfully performed in 1989 as an alternative to prenatal diagnosis for couples at risk of transmitting a genetic or chromosomal abnormality, such as cystic fibrosis, to their child. From embryos generated in vitro, biopsied cells are genetically tested. From the mid-1990s, this technology has been employed as an embryo selection tool for patients undergoing in vitro fertilisation, screening as many chromosomes as possible, in the hope that selecting chromosomally normal embryos will lead to higher implantation and decreased miscarriage rates...
January 1, 2017: Journal of Medical Screening
https://www.readbyqxmd.com/read/28612309/pre-implantation-genetic-diagnosis-should-we-use-icsi-for-all
#4
Baruch Feldman, Adva Aizer, Masha Brengauz, Keren Dotan, Jacob Levron, Eyal Schiff, Raoul Orvieto
OBJECTIVE: Intracytoplasmic sperm injection (ICSI) is commonly used during pre-implantation genetic diagnosis (PGD) in vitro fertilization (IVF), aiming to eliminate the risk of contamination from extraneous sperm DNA. Recently, ICSI "overuse" in non-male infertility has been doubted, since it does not offer an advantage over IVF. Prompted by the aforementioned observations, we sought to assess the accuracy of IVF vs ICSI in PGD cases, as might be reflected by a difference in the prevalence of discarded embryos as a consequent of parental contamination...
June 13, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28608596/live-birth-rate-following-oral-antibiotic-treatment-for-chronic-endometritis-in-infertile-women-with-repeated-implantation-failure
#5
Kotaro Kitaya, Hidehiko Matsubayashi, Yukiko Takaya, Rie Nishiyama, Kohei Yamaguchi, Takumi Takeuchi, Tomomoto Ishikawa
PROBLEM: The aim of this prospective study was to investigate the prevalence of chronic endometritis (CE) in infertile women with a history of repeated implantation failure (RIF) and to determine whether oral antibiotic treatment improves their live birth rate in the following embryo transfer (ET) cycles. METHOD OF STUDY: Endometrial biopsy samples obtained from infertile women with RIF were subjected to immunohistochemistrical/histopathologic diagnosis of CE. Following antibiotic administration to the RIF/CE group, their histopathologic cure rate, microbial detection rate, and reproductive outcome in the subsequent ET cycles were prospectively studied...
June 13, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28601378/gnrh-agonist-administration-prior-to-embryo-transfer-in-freeze-all-cycles-of-patients-with-endometriosis-or-aberrant-endometrial-integrin-expression
#6
Eric S Surrey, Mandy Katz-Jaffe, Laxmi V Kondapalli, Robert L Gustofson, William B Schoolcraft
Prolonged gonadotrophin-releasing hormone agonist (GnRHa) administration before IVF with fresh embryo transfer to patients with endometriosis or aberrant endometrial integrin expression (-integrin) improves outcomes but may suppress ovarian response and prevents elective cryopreservation of all embryos. This retrospective cohort pilot study evaluates freeze-all cycles with subsequent prolonged GnRHa before embryo transfer in these populations. Patients from 2010 to 2015 who met inclusion criteria and received a long-acting GnRHa every 28 days twice before FET were evaluated...
May 17, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28591199/sperm-dna-fragmentation-index-does-not-correlate-with-blastocyst-aneuploidy-or-morphological-grading
#7
Itai Gat, Katelynn Tang, Kevin Quach, Valeriy Kuznyetsov, Ran Antes, Melissa Filice, Khaled Zohni, Clifford Librach
High DNA fragmentation index (DFI) may be associated with poor outcome after IVF. Our aim was to determine whether DFI impacts blastocyst quality or clinical outcome. This retrospective study included 134 couples who underwent 177 IVF-ICSI and pre-implantation genetic screening (PGS) cycles during January 1st, 2014-March 31st, 2016 and had documented previous DFI. Group 1 (DFI>30%) encompassed 25 couples who underwent 36 cycles; Group 2 (DFI 15-30%) included 45 couples and 57 cycles; group 3 (DFI<15%) included 64 couples and 83 cycles...
2017: PloS One
https://www.readbyqxmd.com/read/28579407/detailed-investigation-into-the-cytogenetic-constitution-and-pregnancy-outcome-of-replacing-mosaic-blastocysts-detected-with-the-use-of-high-resolution-next-generation-sequencing
#8
Santiago Munné, Joshua Blazek, Michael Large, Pedro A Martinez-Ortiz, Haley Nisson, Emmeline Liu, Nicoletta Tarozzi, Andrea Borini, Amie Becker, John Zhang, Susan Maxwell, James Grifo, Dhruti Babariya, Dagan Wells, Elpida Fragouli
OBJECTIVE: To determine the pregnancy outcome potential of mosaic embryos, detected by means of preimplantation genetic screening (PGS) with the use of next-generation sequencing (NGS). DESIGN: Retrospective study. SETTING: Genetics laboratories. PATIENT(S): PGS cycles during which either mosaic or euploid embryos were replaced. INTERVENTION(S): Blastocysts were biopsied and processed with the use of NGS, followed by frozen embryo transfer...
June 1, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28577183/evaluation-of-comprehensive-chromosome-screening-platforms-for-the-detection-of-mosaic-segmental-aneuploidy
#9
David Goodrich, Tongji Xing, Xin Tao, Agnieszka Lonczak, Yiping Zhan, Jessica Landis, Rebekah Zimmerman, Richard T Scott, Nathan R Treff
PURPOSE: A subset of preimplantation embryos identified as euploid may in fact possess both whole and sub-chromosomal mosaicism, raising concerns regarding the predictive value of current comprehensive chromosome screening (CCS) methods utilizing a single biopsy. Current CCS methods may be capable of detecting sub-chromosomal mosaicism in a trophectoderm biopsy by examining intermediate levels of segmental aneuploidy within a biopsy. This study evaluates the sensitivity and specificity of segmental aneuploidy detection by three commercially available CCS platforms utilizing a cell line mixture model of segmental mosaicism in a six-cell trophectoderm biopsy...
June 2, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28576301/obstetric-and-neonatal-outcomes-of-pregnancies-conceived-after-preimplantation-genetic-diagnosis-cohort-study-and-meta-analysis
#10
Joseph Hasson, Dana Limoni, Mira Malcov, Tsvia Frumkin, Hadar Amir, Tal Shavit, BjØrn Bay, Ariel Many, Benjamin Almog
Preimplantation genetic diagnosis (PGD) may pose risks to pregnancy outcome owing to the invasiveness of the biopsy procedure. This study compares outcome of singleton and twin clinical pregnancies conceived after fresh embryo transfers of PGD (n = 89) and matched intracytoplasmic sperm injection (ICSI) pregnancies (n = 166). The study was carried out in a single university affiliated centre. Because of the paucity of available data, a literature-based meta-analysis of studies comparing neonatal outcome of PGD and ICSI pregnancies was also conducted...
May 18, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28560610/association-between-growth-dynamics-morphological-parameters-the-chromosomal-status-of-the-blastocysts-and-clinical-outcomes-in-ivf-pgs-cycles-with-single-embryo-transfer
#11
Oleksii O Barash, Kristen A Ivani, Susan P Willman, Evan M Rosenbluth, Deborah S Wachs, Mary D Hinckley, Sara Pittenger Reid, Louis N Weckstein
PURPOSE: The purpose of the present study is to examine interconnection between speed of embryo development, the genetic status of the blastocysts, and clinical outcomes in IVF preimplantation genetic screening (PGS) cycles with single embryo transfer (SET). METHODS: The retrospective comparative study has been performed between January 2013 and January 2016. Seven hundred thirty-seven cycles of IVF treatment with PGS, followed by 503 SETs, were included in the study...
May 30, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28555358/complex-chromosomal-rearrangement-a-lesson-learned-from-pgs
#12
Tsvia Frumkin, Sagit Peleg, Veronica Gold, Adi Reches, Shiri Asaf, Foad Azem, Dalit Ben-Yosef, Mira Malcov
PURPOSE: The aim of the study is to report a case of non-diagnosed complex chromosomal rearrangement (CCR) identified by preimplantation genetic screening (PGS) followed by preimplantation genetic diagnosis (PGD) which resulted in a pregnancy and delivery of healthy offspring. METHODS: A 29-year-old woman and her spouse, both diagnosed previously with normal karyotypes, approached our IVF-PGD center following eight early spontaneous miscarriages. PGS using chromosomal microarray analysis (CMA) was performed on biopsied trophectoderm...
May 29, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28548200/live-birth-after-transfer-of-vitrified-warmed-embryo-derived-from-vitrified-warmed-oocyte-and-frozen-thawed-sperm-following-failed-icsi-case-report
#13
Zhihong Zhang, Jihong Zhu, Hongyang Zhang, Qi Xi, Dongfeng Geng, Shu Deng, Ruizhi Liu
Since the first successful pregnancy from a frozen human oocyte was reported, remarkable technological progress has been made in the area of cryopreservation of human oocytes. We report a successful delivery of two healthy babies after transfer of vitrified-warmed embryos derived from intracytoplasmic sperm injection (ICSI) with vitrified-warmed oocytes and frozen-thawed sperm. A female patient and her husband with severe oligoasthenspermia are reported. At the day of oocyte collection, very few inactive sperms were found in her husband semen...
March 2017: Revista Médica de Chile
https://www.readbyqxmd.com/read/28537237/-comparative-results-of-preimplantation-genetic-screening-by-array-comparative-genomic-hybridization-and-new-generation-sequencing
#14
N V Aleksandrova, E S Shubina, A N Ekimov, T A Kodyleva, I S Mukosey, N P Makarova, E V Kulakova, L A Levkov, I Yu Barkov, D Yu Trofimov, G T Sukhikh
Aneuploidies as quantitative chromosome abnormalities are a main cause of failed development of morphologically normal embryos, implantation failures, and early reproductive losses. Preimplantation genetic screening (PGS) allows a preselection of embryos with a normal karyotype, thus increasing the implantation rate and reducing the frequency of early pregnancy loss after IVF. Modern PGS technologies are based on a genome-wide analysis of the embryo. The first pilot study in Russia was performed to assess the possibility of using semiconductor new-generation sequencing (NGS) as a PGS method...
March 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28534057/the-effect-of-thawing-protocols-on-follicle-conservation-in-human-ovarian-tissue-cryopreservation
#15
T Morewood, N Getreu, B Fuller, J Morris, P Hardiman
BACKGROUND: Ovarian tissue cryopreservation has the potential to improve fertility preservation for a growing number of patients undergoing sterilising therapy, particularly where oocyte or embryo cryopreservation is not suitable. However, its success is limited by significant follicular apoptosis upon thawing, and there is wide variation in thawing protocols used with little evidence of efficacy. OBJECTIVE: To determine the best warming rates to maintain tissue viability...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28501368/contribution-of-immunology-to-implantation-failure-of-euploid-embryos
#16
REVIEW
Jason M Franasiak, Richard T Scott
Outcomes in assisted reproduction have seen marked improvement. With increased ability in the embryology laboratory to use extended embryo culture which in turn enables other selective techniques, such as trophectoderm biopsy and comprehensive chromosome screening, the chance of success per embryo transfer is increased. However, even the selection of a euploid blastocyst, which selects out many embryonic factors, does not yield successful implantation and ultimately delivery in all cases. Among the factors that affect implantation failure of apparently reproductively competent embryos, the immune system has been perhaps both the most plausible and the most debated...
June 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28498723/advances-in-preimplantation-genetic-testing-for-monogenic-disease-and-aneuploidy
#17
Nathan R Treff, Rebekah S Zimmerman
Genetic testing of preimplantation embryos promises to prevent monogenic disease in children born to at-risk couples, the transfer of unbalanced embryos to patients carrying a balanced translocation, and the use of aneuploid embryos created during in vitro fertilization. Technologies have evolved from fluorescence in situ hybridization to next-generation-sequencing-based aneuploidy screening and allow for simultaneous testing of multiple genetic abnormalities in a single biopsy. The field has also shifted away from polar body or blastomere biopsy and toward trophectoderm biopsy as the new standard...
May 12, 2017: Annual Review of Genomics and Human Genetics
https://www.readbyqxmd.com/read/28497410/could-monopronucleated-icsi-zygotes-be-considered-for-transfer-analysis-through-time-lapse-monitoring-and-pgs
#18
S Mateo, F Vidal, M Parriego, I Rodríguez, V Montalvo, A Veiga, M Boada
PURPOSE: The purpose of this study was to investigate the chromosomal constitution and the developmental potential of intracytoplasmic sperm injection (ICSI) deriving embryos displaying a single pronucleus at the zygote stage. METHODS: Eighty-eight embryos from single pronucleus (1PN) two polar bodies (2PB) ICSI zygotes from 64 preimplantational genetic screening (PGS) cycles (October 2012-December 2014), were retrospectively analyzed. Zygotes were cultured in a time-lapse incubator...
May 11, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28479756/relationship-between-morphology-euploidy-and-implantation-potential-of-cleavage-and-blastocyst-stage-embryos
#19
Gaurav Majumdar, Abha Majumdar, Ishwar C Verma, Kailash C Upadhyaya
AIM: The aim of this study was to investigate the relationship between the morphology, euploidy and implantation rate of cleavage stage and blastocyst stage embryos. SETTING: Institution-based, tertiary care in-vitro fertilization centre. STUDY DESIGN: This study included a retrospective data analysis of 306 embryos: 154 cleavage stage embryos and 152 blastocysts that underwent biopsy on day 3 and day 5/6, respectively, which were subsequently screened for aneuploidy by array comparative genomic hybridization analysis...
January 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28451773/correlation-between-three-dimensional-power-doppler-and-morphometric-measurement-of-endometrial-vascularity-at-the-time-of-embryo-implantation-in-women-with-unexplained-recurrent-miscarriage
#20
Xiaoyan Chen, Sotirios H Saravelos, Yingyu Liu, Jin Huang, Chi Chiu Wang, Tin Chiu Li
Power Doppler in combination with three-dimensional (3D-PD) ultrasonography has been used as a noninvasive tool to evaluate the vascularity. However, it is unclear whether 3D-PD can accurately reflect endometrial vascularization and replace the invasive endometrial biopsy. This study aims to investigate the correlation between 3D-PD and micro vessel morphometric measurement of endometrial vascularity. Twenty-five women with unexplained recurrent miscarriage were recruited for 3D-PD and endometrial biopsy on precisely day LH + 7...
April 27, 2017: Journal of Molecular Histology
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