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https://www.readbyqxmd.com/read/29156720/novel-strategies-of-raman-imaging-for-brain-tumor-research
#1
Imiela Anna, Polis Bartosz, Polis Lech, Abramczyk Halina
Raman diagnostics and imaging have been shown to be an effective tool for the analysis and discrimination of human brain tumors from normal structures. Raman spectroscopic methods have potential to be applied in clinical practice as they allow for identification of tumor margins during surgery. In this study, we investigate medulloblastoma (grade IV WHO) (n= 5), low-grade astrocytoma (grades I-II WHO) (n =4), ependymoma (n=3) and metastatic brain tumors (n= 1) and the tissue from the negative margins used as normal controls...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156404/cascaded-signal-amplification-via-target-triggered-formation-of-aptazyme-for-sensitive-electrochemical-detection-of-atp
#2
Xia Li, Jianmei Yang, Jiaqing Xie, Bingying Jiang, Ruo Yuan, Yun Xiang
The construction of reliable sensors for adenosine triphosphate (ATP) detection gains increasing interest because of its important roles in various enzymatic activities and biological processes. Based on a cascaded, significant signal amplification approach by the integration of the aptazymes and catalytic hairpin assembly (CHA), we have developed a sensitive electrochemical sensor for the detection of ATP. The target ATP leads to the conformational change of the aptazyme sequences and their association with the hairpin substrates to form active aptazymes, in which the hairpin substrates are cyclically cleaved by the metal ion cofactors in buffer to release the enzymatic sequences that can also bind the hairpin substrates to generate active DNAzymes...
November 17, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29156087/fundamental-nursing-care-a-systematic-review-of-the-evidence-on-the-effect-of-nursing-care-interventions-for-nutrition-elimination-mobility-and-hygiene
#3
David A Richards, Angelique Hilli, Claire Pentecost, Victoria A Goodwin, Julia Frost
AIMS AND OBJECTIVES: To determine the effects of nursing interventions for people's nutrition, elimination, mobility and hygiene needs. BACKGROUND: Patient experience of health care is sensitive to nursing quality. A refocus on fundamental nursing care is undermined by lack of evidence of effectiveness for interventions in core areas such as elimination, nutrition, mobility and hygiene. DESIGN: SYSTEMATIC REVIEW: METHODS: We searched for and included experimental studies on interventions by professionally qualified and unregistered nurses that addressed participants' nutrition, elimination, mobility and hygiene needs...
November 20, 2017: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/29155905/adsorption-of-three-domain-antifreeze-proteins-on-ice-a-study-using-lgmmas-theory-and-monte-carlo-simulations
#4
Juan Ignacio Lopez Ortiz, Paola Torres, Evelina Quiroga, Claudio F Narambuena, Antonio J Ramirez-Pastor
In the present work, the adsorption of three-domain antifreeze proteins on ice is studied by combining a statistical thermodynamics based theory and Monte Carlo simulations. The three-domain protein is modeled by a trimer, and the ice surface is represented by a lattice of adsorption sites. The statistical theory, obtained from the exact partition function of non-interacting trimers adsorbed in one dimension and its extension to two dimensions, includes the configuration of the molecule in the adsorbed state, and allows the existence of multiple adsorption states for the protein...
November 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29155892/derivation-and-application-of-an-analytical-rock-displacement-solution-on-rectangular-cavern-wall-using-the-inverse-mapping-method
#5
Mingzhong Gao, Bin Yu, Zhiqiang Qiu, Xiangang Yin, Shengwei Li, Qiang Liu
Rectangular caverns are increasingly used in underground engineering projects, the failure mechanism of rectangular cavern wall rock is significantly different as a result of the cross-sectional shape and variations in wall stress distributions. However, the conventional computational method always results in a long-winded computational process and multiple displacement solutions of internal rectangular wall rock. This paper uses a Laurent series complex method to obtain a mapping function expression based on complex variable function theory and conformal transformation...
2017: PloS One
https://www.readbyqxmd.com/read/29155887/effects-of-an-a%C3%AE-antibody-fragment-on-a%C3%AE-aggregation-and-astrocytic-uptake-are-modulated-by-apolipoprotein-e-and-j-mimetic-peptides
#6
Laia Montoliu-Gaya, Sandra D Mulder, Robert Veerhuis, Sandra Villegas
Aβ-Immunotherapy has long been studied in the treatment of Alzheimer's disease (AD), but not how other molecules involved in the disease can affect antibody performance. We previously designed an antibody fragment, scFv-h3D6, and showed that it precludes Aβ-induced cytotoxicity by withdrawing Aβ oligomers from the amyloid pathway towards a non-toxic, worm-like pathway. ScFv-h3D6 was effective at the behavioral, cellular, and molecular levels in the 3xTg-AD mouse model. Because scFv-h3D6 treatment restored apolipoprotein E (apoE) and J (apoJ) concentrations to non-pathological values, and Aβ internalization by glial cells was found to be decreased in the presence of these apolipoproteins, we now aimed to test the influence of scFv-h3D6 on Aβ aggregation and cellular uptake by primary human astrocytes in the presence of therapeutic apoE and apoJ mimetic peptides (MPs)...
2017: PloS One
https://www.readbyqxmd.com/read/29155738/imaging-amyloid-tissues-stained-with-luminescent-conjugated-oligothiophenes-by-hyperspectral-confocal-microscopy-and-fluorescence-lifetime-imaging
#7
Sofie Nyström, Marcus Bäck, K Peter R Nilsson, Per Hammarström
Proteins that deposit as amyloid in tissues throughout the body can be the cause or consequence of a large number of diseases. Among these we find neurodegenerative diseases such as Alzheimer's and Parkinson's disease afflicting primarily the central nervous system, and systemic amyloidosis where serum amyloid A, transthyretin and IgG light chains deposit as amyloid in liver, carpal tunnel, spleen, kidney, heart, and other peripheral tissues. Amyloid has been known and studied for more than a century, often using amyloid specific dyes such as Congo red and Thioflavin T (ThT) or Thioflavin (ThS)...
October 20, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155589/the-reaction-mechanism-of-isopentenyl-phosphate-kinase-a-qm-mm-study
#8
James McClory, David J Timson, Warispreet Singh, Jian Zhang, Meilan Huang
Isopentenyl phosphate kinase (IPK) catalyzes the Mg2+-ATP dependent phosphorylation reactions to produce isopentenyl diphosphate, an important precursor in the synthesis of isopentenols. However, the position of the divalent metal ion in the crystal structures of IPK in complex with ATP and its native substrate IP has not been definitively resolved, and as a result ambiguity surrounds the catalytic mechanism of IP, limiting its exploitation as a biofuel and in drug design. Here we report the conformational change caused by substrate binding and the catalytically competent structure in complex with the metal ion Mg2+ using molecular dynamic simulations and density functional theory-based quantum mechanics/molecular mechanics calculations (B97d/AMBER99)...
November 20, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29155585/allosteric-inhibitors-of-shp2-with-therapeutic-potential-for-cancer-treatment
#9
Jingjing Xie, Xiaojia Si, Shoulai Gu, Mingliang Wang, Jian Shen, Haoyan Li, Jian Shen, Dan Li, Yanjia Fang, Cong Liu, Jidong Zhu
SHP2, a cytoplasmic protein-tyrosine phosphatase encoded by the PTPN11 gene, is involved in multiple cell signaling processes including Ras/MAPK and Hippo/YAP pathways. SHP2 has been shown to contribute to the progression of a number of cancer types including leukemia, gastric and breast cancer. It also regulates T-cell activation by interacting with inhibitory immune checkpoint receptors such as the programmed cell death 1 (PD-1) and B- and T-lymphocyte attenuator (BTLA). Thus, SHP2 inhibitors have drawn great attention by both inhibiting tumor cell proliferation and activating T cell immune responses toward cancer cells...
November 20, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29155581/phosphorescence-control-mediated-by-molecular-rotation-and-aurophilic-interactions-in-amphidynamic-crystals-of-1-4-bis-tri-p-fluorophenyl-phosphane-gold-i-ethynyl-benzene
#10
Mingoo Jin, Tim S Chung, Tomohiro Seki, Hajime Ito, Miguel A Garcia-Garibay
Here we present a structural design aimed at the control of phosphorescence emission as the result of changes in molecular rotation in a crystalline material. The proposed strategy includes the use of aurophilic interactions, both as a crystal engineering tool and as sensitive emission probe, and the use of a dumbbell-shaped architecture intended to create a low packing density region that permits the rotation of a central phenylene. Molecular rotor 1 with of a central 1,4-diethynylphenylene rotator linked to two gold(I) triphenylphosphane complexes was prepared and its structure confirmed by single crystal X-ray diffraction (XRD), which revealed chains mediated by dimeric aurophilic interactions...
November 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29155569/redox-behavior-of-a-dinuclear-ruthenium-ii-complex-bearing-an-uncommon-bridging-ligand-insights-from-high-pressure-electrochemistry
#11
Maximilian Dürr, Johannes Klein, Axel Kahnt, Sabine Becker, Ralph Puchta, Biprajit Sarkar, Ivana Ivanović-Burmazović
A dinuclear ruthenium complex bridged by 2,3,5,6-pyrazinetetracarboxylic acid (μ-LH2(2-)) was synthesized and characterized by X-ray crystallography, cyclic voltammetry under ambient and elevated pressures, electron paramagnetic resonance (EPR) and UV/vis-NIR (NIR = near-infrared) spectroelectrochemistry, pulse radiolysis, and computational methods. We probed for the first time in the field of mixed-valency the use of high-pressure electrochemical methods. The investigations were directed toward the influence of the protonation state of the bridging ligand on the electronic communication between the ruthenium ions, since such behavior is interesting in terms of modulating redox chemistry by pH...
November 20, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29155366/applications-of-stimuli-responsive-nanoscale-drug-delivery-systems-in-translational-research
#12
REVIEW
Mengjie Gu, Xin Wang, Tan Boon Toh, Edward Kai-Hua Chow
Nanoscale drug delivery systems or nanocarriers have shown tremendous promise in the target-specific delivery of therapeutics as well as diagnostic agents. Additional properties can be introduced into nanocarriers to enhance the bioavailability and targeting efficiency of the transported drugs at diseased sites. Such nanocarriers are usually incorporated with stimuli-responsive components that can be triggered by specific stimuli (e.g., temperature, pH, or enzymes) and further induced by certain biological responses, such as enzyme hydrolysis and molecular conformational changes, leading to the controlled release of the transported molecules at targeted sites...
November 16, 2017: Drug Discovery Today
https://www.readbyqxmd.com/read/29155312/designing-clinical-studies-in-orthopedic-traumatology
#13
REVIEW
D M Rouleau
The design of clinical studies in orthopedic traumatology is challenging in several respects. In this era of evidence-based medicine, the pressure is high to choose our treatments solely based on randomized controlled studies. This type of study, when well-constructed, makes it possible to discern the best treatment for a specific fracture in a given group of patients and in connection with a specific outcome. Randomized controlled trials require a lot of resources and are not designed to answer all research questions...
November 16, 2017: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/29155216/communication-between-genomic-and-non-genomic-signaling-events-coordinate-steroid-hormone-actions
#14
Sandi R Wilkenfeld, Chenchu Lin, Daniel E Frigo
Steroid hormones are lipophilic molecules produced in one cell that can travel great distances within the body to elicit biological effects in another cell. In the canonical pathway, steroid hormone binding to a nuclear receptor (NR), often in the cytoplasm, causes the receptor to undergo a conformational change and translocate to the nucleus, where it interacts with specific sequences of DNA to regulate transcription. In addition to the classical genomic mechanism of action, alternate mechanisms of steroid activity have emerged that involve rapid, non-genomic signaling...
November 16, 2017: Steroids
https://www.readbyqxmd.com/read/29155203/the-hydrogen-bond-network-around-glu160-contributes-to-the-structural-stability-of-chitosanase-csna-from-renibacterium-sp-qd1
#15
Yujuan Han, Rilei Yu, Peixin Gao, Xinzhi Lu, Wengong Yu
CsnA, a chitosanase from Renibacterium sp. QD1, has great potential for industrial applications due to its high yield and broad pH stability. In this study, a specific Glu160 in CsnA was identified by sequence alignment, and structural analysis and MD simulation predicted that Glu160 formed a hydrogen-bond network with Lys163 and Thr114. To evaluate the effect of the network, we constructed four mutants, including E160A, E160Q, K163A, and T114A, which partially or completely destroy this network. Characterization of these mutants demonstrated that the disruption of the network significantly decreased the enzyme thermostability...
November 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29155132/stiffening-of-flexible-sumo1-protein-upon-peptide-binding-analysis-with-anisotropic-network-model
#16
Ranja Sarkar
SUMO (small ubiquitin-like modifier) proteins interact with a large number of target proteins via a key regulatory event called sumoylation that encompasses activation, conjugation and ligation of SUMO proteins through specific E1, E2, and E3-type enzymes respectively. Single-molecule atomic force microscopic (AFM) experiments performed to unravel bound SUMO1 along its N-C termini direction reveal that E3-ligases (in the form of small peptides) increase mechanical stability (along the axis) of the flexible protein upon binding...
November 16, 2017: Mathematical Biosciences
https://www.readbyqxmd.com/read/29155071/structural-rearrangements-of-the-rna-polymerase-iii-machinery-during-trna-transcription-initiation
#17
REVIEW
Ewan Phillip Ramsay, Alessandro Vannini
RNA polymerase III catalyses the synthesis of tRNAs in eukaryotic organisms. Through combined biochemical and structural characterisation, multiple auxiliary factors have been identified alongside RNA Polymerase III as critical in both facilitating and regulating transcription. Together, this machinery forms dynamic multi-protein complexes at tRNA genes which are required for polymerase recruitment, DNA opening and initiation and elongation of the tRNA transcripts. Central to the function of these complexes is their ability to undergo multiple conformational changes and rearrangements that regulate each step...
November 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29154993/serration-pattern-analysis-for-differentiating-epidermolysis-bullosa-acquisita-from-other-pemphigoid-diseases
#18
Joost M Meijer, Ingeborg Atefi, Gilles F H Diercks, Artem Vorobyev, Janny Zuiderveen, Hillegonda J Meijer, Hendri H Pas, Detlef Zillikens, Enno Schmidt, Marcel F Jonkman
BACKGROUND: Direct immunofluorescence microscopy (DIF) of a skin biopsy specimen is the reference standard for the diagnosis of pemphigoid diseases (PD). Serration pattern analysis enables differentiation of epidermolysis bullosa acquisita (EBA) from other PD using DIF microscopy alone. However, practice gaps need to be addressed for implication of this technique in daily routine diagnostics. OBJECTIVE: to determine and optimize the technical requirements for serration pattern analysis of DIF microscopy and determine inter-rater conformity of serration pattern analysis...
November 15, 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/29154882/interaction-of-novel-twin-tailed-oxy-diester-functionalized-surfactant-with-lysozyme-spectroscopic-and-computational-perspective
#19
Imtiyaz Ahmad Bhat, Waseem Feeroze Bhat, Mohd Akram, Kabir-Ud-Din
Herein, we have examined the interaction of oxy-diester novel twin tailed (gemini) surfactant, 2,2(')-[(oxybis(ethane-1,2-diyl))bis(oxy)]bis(N-hexadecyl-N,Ndimethyl-2-oxoethanaminium) dichloride (C16-E2O-C16) with hen egg white lysozyme (HEWL), utilizing a spectroscopic and molecular docking techniques. Steady-state fluorescence infers ground state C16-E2O-C16-HEWL complex formation. Other spectroscopic results validated the conformational, structural and micro-environmental changes in HEWL upon interaction with C16-E2O-C16...
November 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29154742/variation-in-the-toll-like-receptor-4-tlr4-gene-affects-milk-traits-in-dairy-cows
#20
Huitong Zhou, Long Cheng, Hua Gong, Seung O Byun, Grant R Edwards, Jon G H Hickford
The objective of this Research Communication was to use polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analysis to investigate a region of the bovine TLR4 gene (TLR4) in pasture-fed New Zealand (NZ) Holstein-Friesian × Jersey (HF × J) cross dairy cows and to determine whether gene variation was associated with milk production traits. Genetic variation was observed, with two variants (A and B) containing a single nucleotide polymorphism (SNP) (c.2021C/T) that was non-synonymous and putatively results in a p...
November 2017: Journal of Dairy Research
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