keyword
MENU ▼
Read by QxMD icon Read
search

CAS

keyword
https://www.readbyqxmd.com/read/28636531/nedd9-an-independent-good-prognostic-factor-in-intermediaterisk-acute-myeloid-leukemia-patients
#1
Victor Pallarès, Montserrat Hoyos, M Carmen Chillón, Eva Barragán, M Isabel Prieto Conde, Marta Llop, María Virtudes Céspedes, Josep F Nomdedeu, Salut Brunet, Miguel Ángel Sanz, Marcos González-Díaz, Jorge Sierra, Isolda Casanova, Ramon Mangues
Intermediate-risk acute myeloid leukemia (IR-AML) is the largest subgroup of AML patients and is highly heterogeneous. Whereas adverse and favourable risk patients have well-established treatment protocols, IR-AML patients have not. It is, therefore, crucial to find novel factors that stratify this subgroup to implement risk-adapted strategies. The CAS (Crk-associated substrate) adaptor protein family regulates cell proliferation, survival, migration and adhesion. Despite its association with metastatic dissemination and prognosis of different solid tumors, the role of these proteins in hematological malignancies has been scarcely evaluated...
June 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28634692/crispr-cas-orthologues-and-variants-optimizing-the-repertoire-specificity-and-delivery-of-genome-engineering-tools
#2
Alberto Cebrian-Serrano, Benjamin Davies
Robust and cost-effective genome editing in a diverse array of cells and model organisms is now possible thanks to the discovery of the RNA-guided endonucleases of the CRISPR-Cas system. The commonly used Cas9 of Streptococcus pyogenes shows high levels of activity but, depending on the application, has been associated with some shortcomings. Firstly, the enzyme has been shown to cause mutagenesis at genomic sequences resembling the target sequence. Secondly, the stringent requirement for a specific motif adjacent to the selected target site can limit the target range of this enzyme...
June 20, 2017: Mammalian Genome: Official Journal of the International Mammalian Genome Society
https://www.readbyqxmd.com/read/28634160/high-throughput-characterization-of-cascade-type-i-e-crispr-guide-efficacy-reveals-unexpected-pam-diversity-and-target-sequence-preferences
#3
Becky Xu Hua Fu, Michael Wainberg, Anshul Kundaje, Andrew Z Fire
Interactions between Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) RNAs and CRISPR-associated (Cas) proteins form an RNA-guided adaptive immune system in prokaryotes. The adaptive immune system utilizes segments of the genetic material of invasive foreign elements in the CRISPR locus. The loci are transcribed and processed to produce small CRISPR RNAs (crRNAs), with degradation of invading genetic material directed by a combination of complementarity between RNA and DNA and in some cases recognition of adjacent motifs called PAMs (Protospacer Adjacent Motifs)...
June 20, 2017: Genetics
https://www.readbyqxmd.com/read/28633304/molecular-aspects-of-the-biophysical-co2-concentrating-mechanism-and-its-regulation-in-marine-diatoms
#4
Yoshinori Tsuji, Kensuke Nakajima, Yusuke Matsuda
Diatoms operate a CO2-concentrating mechanism (CCM) that drives upwards of 20% of annual global primary production. Recent progress in CCM research in the marine pennate diatom Phaeodactylum tricornutum revealed that this diatom directly takes up HCO3- from seawater through low-CO2-inducible plasma membrane HCO3- transporters, which belong to the solute carrier (SLC) 4 family. Apart from this, studies of carbonic anhydrases (CAs) in diatoms have revealed considerable diversity in classes and localization among species...
June 13, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28633080/disruptive-non-disruptive-applications-of-crispr-cas9
#5
REVIEW
Jonathan L Schmid-Burgk
The bacterial type II Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR Associated (Cas) systems, and in particular Streptococcus pyogenes CRISPR-Cas9, have been broadly applied to edit the genome of bacterial and eukaryotic cells. Cas9, which is an RNA-guided programmable nuclease, is a powerful tool for disrupting protein-coding genes. Cas9 cleaves target sites to generate a double-strand break (DSB) that is repaired via an error-prone repair process, leading to insertion/deletion mutations and gene knockouts...
June 17, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28632941/systematic-review-of-carotid-artery-procedures-and-the-volume-outcome-relationship-in-europe
#6
REVIEW
P Phillips, E Poku, M Essat, H B Woods, E A Goka, E C Kaltenthaler, P Shackley, S Walters, J A Michaels
BACKGROUND: Hospitals that conduct more procedures on the carotid arteries may achieve better outcomes. In the context of ongoing reconfiguration of UK vascular services, this systematic review was conducted to evaluate the relationship between the volume of carotid procedures and outcomes, including mortality and stroke. METHODS: Searches of electronic databases identified studies that reported the effect of hospital or clinician volume on outcomes. Reference and citation searches were also performed...
June 20, 2017: British Journal of Surgery
https://www.readbyqxmd.com/read/28630903/comment-1-on-formation-of-the-isthmus-of-panama-by-o-dea-et-al
#7
Carlos Jaramillo, Camilo Montes, Agustín Cardona, Daniele Silvestro, Alexandre Antonelli, Christine D Bacon
A review and reanalysis of geological, molecular, and paleontological data led O'Dea et al. (1) to propose (i) that reports by Montes et al. (2) and Bacon et al. (3) regarding a middle Miocene closure of the Central American Seaway (CAS) are unsupported, and (ii) a new age of the formation of the Isthmus at 2.8 million years ago (Ma). Here, we reject both of these conclusions.
June 2017: Science Advances
https://www.readbyqxmd.com/read/28630534/data-sharing-a-new-editorial-initiative-of-the-international-committee-of-medical-journal-editors-implications-for-the-editors-network
#8
Fernando Alfonso, Karlen Adamyan, Jean-Yves Artigou, Michael Aschermann, Michael Boehm, Alfonso Buendia, Pao-Hsien Chu, Ariel Cohen, Livio Dei Cas, Mirza Dilic, Anton Doubell, Dario Echeverri, Nuray Enç, Ignacio Ferreira-González, Krzysztof J Filipiak, Andreas Flammer, Eckart Fleck, Plamen Gatzov, Carmen Ginghina, Lino Goncalves, Habib Haouala, Mahmoud Hassanein, Gerd Heusch, Kurt Huber, Ivan Hulín, Mario Ivanusa, Rungroj Krittayaphong, Chu-Pak Lau, Germanas Marinskis, François Mach, Luiz Felipe Moreira, Tuomo Nieminen, Latifa Oukerraj, Stefan Perings, Luc Pierard, Tatjana Potpara, Walter Reyes-Caorsi, Se-Joong Rim, Olaf Rødevand, Georges Saade, Mikael Sander, Evgeny Shlyakhto, Bilgin Timuralp, Dimitris Tousoulis, Dilek Ural, J J Piek, Albert Varga, Thomas F Lüscher
The International Committee of Medical Journal Editors (ICMJE) provides recommendations to improve the editorial standards and scientific quality of biomedical journals. These recommendations range from uniform technical requirements to more complex and elusive editorial issues including ethical aspects of the scientific process. Recently, registration of clinical trials, conflicts of interest disclosure, and new criteria for authorship- emphasizing the importance of responsibility and accountability-, have been proposed...
May 2017: Acta Cardiologica Sinica
https://www.readbyqxmd.com/read/28628499/psychometric-validation-of-the-mandarin-version-control-attitudes-scale-revised-questionnaire-in-taiwanese-patients-with-heart-failure
#9
Tsuey-Yuan Huang
BACKGROUND: Perceived control is an important construct in patients with heart failure (HF) and related to improved health outcomes. There is no reliable and valid instrument available for the measurement of perceived control in Taiwan, limiting the practical application of the construct in research and clinical practice. PURPOSE: The aim of this study was to translate, culturally adapt, and validate the Mandarin version of the Control Attitudes Scale-Revised (Mandarin CAS-R) in Taiwanese patients with HF...
June 17, 2017: Journal of Cardiovascular Nursing
https://www.readbyqxmd.com/read/28627432/high-diversity-of-multidrug-resistant-mycobacterium-tuberculosis-central-asian-strain-isolates-in-nepal
#10
Yogendra Shah, Bhagwan Maharjan, Jeewan Thapa, Ajay Poudel, Hassan Mahmoud Diab, Basu Dev Pandey, Eddie Solo, Norikazu Isoda, Yasuhiko Suzuki, Chie Nakajima
OBJECTIVES: Tuberculosis caused by Mycobacterium tuberculosis (MTB) poses a major public health problem in Nepal. Although it has been reported as one of the dominant genotypes of MTB in Nepal, little information on the Central Asian Strain (CAS) family is available, especially isolates related to multidrug resistance (MDR) cases. Here, we aimed to elucidate the genetic and epidemiological characteristics of MDR CAS isolates in Nepal. METHODS: A total of 145 MDR CAS isolates collected in Nepal from 2008 to 2013 were characterized by spoligotyping, mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) analysis and drug resistance-associating gene sequencing...
June 13, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28627393/the-potential-and-challenges-of-crispr-cas-in-eradication-of-hepatitis-b-virus-covalently-closed-circular-dna
#11
REVIEW
Hung-Chih Yang, Pei-Jer Chen
Current antiviral therapy fails to cure chronic hepatitis B virus (HBV) infection, primarily because of the persistence of covalently closed circular DNA (cccDNA). Although nucleos(t)ide analogues (NAs) can inhibit the reverse transcriptase of HBV and suppress its replication to levels below the detection limit, viremia often rebounds after cessation of therapy. Nuclear cccDNA serves as the HBV replicative template and exhibits extraordinary stability, and is not affected by NAs. Therefore, curing chronic hepatitis B (CHB) requires novel therapy for purging cccDNA from patients...
June 13, 2017: Virus Research
https://www.readbyqxmd.com/read/28625879/rifm-fragrance-ingredient-safety-assessment-benzenepropanenitrile-4-ethyl-%C3%AE-%C3%AE-dimethyl-cas-registry-number-134123-93-6
#12
A M Api, D Belsito, D Botelho, D Browne, M Bruze, G A Burton, J Buschmann, M L Dagli, M Date, W Dekant, C Deodhar, A D Fryer, K Joshi, S La Cava, A Lapczynski, D C Liebler, D O'Brien, R Parakhia, A Patel, T M Penning, G Ritacco, J Romine, D Salvito, T W Schultz, I G Sipes, Y Thakkar, Y Tokura, S Tsang, J Wahler
No abstract text is available yet for this article.
June 15, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28625878/rifm-fragrance-ingredient-safety-assessment-methyl-hexyl-oxo-cyclopentanone-carboxylate-cas-registry-number-37172-53-5
#13
A M Api, D Belsito, S Bhatia, D Botelho, D Browne, M Bruze, G A Burton, J Buschmann, P Calow, M L Dagli, M Date, W Dekant, C Deodhar, A D Fryer, K Joshi, L Kromidas, S La Cava, J F Lalko, A Lapczynski, D C Liebler, D O'Brien, R Parakhia, A Patel, T M Penning, V T Politano, G Ritacco, J Romine, D Salvito, T W Schultz, J Shen, I G Sipes, Y Thakkar, S Tsang, J Wahler, B Wall, D K Wilcox
No abstract text is available yet for this article.
June 15, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28625877/rifm-fragrance-ingredient-safety-assessment-isodecyl-alcohol-cas-registry-number-25339-17-7
#14
A M Api, D Belsito, D Botelho, D Browne, M Bruze, G A Burton, J Buschmann, M L Dagli, M Date, W Dekant, C Deodhar, A D Fryer, K Joshi, S La Cava, A Lapczynski, D C Liebler, D O'brien, R Parakhia, A Patel, T M Penning, G Ritacco, J Romine, D Salvito, T W Schultz, I G Sipes, Y Thakkar, Y Tokura, S Tsang, J Wahler
No abstract text is available yet for this article.
June 15, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28624550/comparative-genome-analysis-of-fish-pathogen-flavobacterium-columnare-reveals-extensive-sequence-diversity-within-the-species
#15
Pattanapon Kayansamruaj, Ha Thanh Dong, Ikuo Hirono, Hidehiro Kondo, Saengchan Senapin, Channarong Rodkhum
Flavobacterium columnare is one of the deadliest fish pathogens causing devastating mortality in various freshwater fish species globally. To gain an insight into bacterial genomic contents and structures, comparative genome analyses were performed using the reference and newly sequenced genomes of F. columnare including genomovar I, II and I/II strains isolated from Thailand, Europe and the USA. Bacterial genomes varied in size from 3.09 to 3.39Mb (2714 to 3101 CDSs). The pan-genome analysis revealed open pan-genome nature of F...
June 14, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28624224/optimizing-the-dna-donor-template-for-homology-directed-repair-of-double-strand-breaks
#16
Fei Song, Knut Stieger
The CRISPR-Cas (clustered regularly interspaced short palindromic repeats-associated proteins) technology enables rapid and precise genome editing at any desired genomic position in almost all cells and organisms. In this study, we analyzed the impact of different repair templates on the frequency of homology-directed repair (HDR) and non-homologous end joining (NHEJ). We used a stable HEK293 cell line expressing the traffic light reporter (TLR-3) system to quantify HDR and NHEJ events following transfection with Cas9, eight different guide RNAs, and a 1,000 bp donor template generated either as circular plasmid, as linearized plasmid with long 3' or 5' backbone overhang, or as PCR product...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28623719/the-crispr-cas-app-goes-viral
#17
REVIEW
Marie-Laurence Lemay, Philippe Horvath, Sylvain Moineau
If biology laboratories were smartphones, CRISPR-Cas would be the leading app. Nowadays, technology users rely on apps to communicate, get directions, entertain, and more. Likewise, many life scientists now rely on CRISPR-Cas systems to study the interactions between microbes and their viruses, to track strains as well as to modify and modulate genomes. Considering their high level of polymorphism, CRISPR arrays can increase the resolution of a microbial typing scheme. As dynamic systems, they allow the identification and the tracking of specific sequences, which is highly valuable for epidemiological studies...
June 14, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28623378/carotid-stenting-in-neuroradiology-a%C3%A2-short-journey-from-the-past-to-current-debates
#18
REVIEW
Joachim Berkefeld, Marlies Wagner, Richard du Mesnil
After a period of stagnation due to negative results of randomized trials for patients with symptomatic stenosis, the relevance of carotid artery stenting (CAS) is increasing again. CAS has become an important and evidence-based part of interventional stroke treatment. Our ability to manage acute recanalization of carotid occlusions and other niche indications properly led some skeptical neurologists to trust in carotid intervention for elective cases as well. The evolution of CAS from initial euphoria to more realistic views under consideration of trial results and guidelines is reviewed...
June 16, 2017: Clinical Neuroradiology
https://www.readbyqxmd.com/read/28623321/dysbiosis-in-chronic-periodontitis-key-microbial-players-and-interactions-with-the-human-host
#19
Zhi-Luo Deng, Szymon P Szafrański, Michael Jarek, Sabin Bhuju, Irene Wagner-Döbler
Periodontitis is an extremely prevalent disease worldwide and is driven by complex dysbiotic microbiota. Here we analyzed the transcriptional activity of the periodontal pocket microbiota from all domains of life as well as the human host in health and chronic periodontitis. Bacteria showed strong enrichment of 18 KEGG functional modules in chronic periodontitis, including bacterial chemotaxis, flagellar assembly, type III secretion system, type III CRISPR-Cas system, and two component system proteins. Upregulation of these functions was driven by the red-complex pathogens and candidate pathogens, e...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28622636/crispr-based-engineering-of-next-generation-lactic-acid-bacteria
#20
REVIEW
Claudio Hidalgo-Cantabrana, Sarah O'Flaherty, Rodolphe Barrangou
The advent of CRISPR-based technologies has opened new avenues for the development of next-generation food microorganisms and probiotics with enhanced functionalities. Building off two decades of functional genomics studies unraveling the genetic basis for food fermentations and host-probiotic interactions, CRISPR technologies offer a wide range of opportunities to engineer commercially-relevant Lactobacillus and Bifidobacteria. Endogenous CRISPR-Cas systems can be repurposed to enhance gene expression or provide new features to improve host colonization and promote human health...
June 13, 2017: Current Opinion in Microbiology
keyword
keyword
34512
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"