Xing Wu, Yiqing Tao, Yangguang Ren, Zihao Zhang, Yudian Zhao, Yixiang Tian, Yijie Li, Meng Hou, Yulong Guo, Yujie Gong, Yanhua Zhang, Donghua Li, Hong Li, Ruirui Jiang, Guoxi Li, Xiaojun Liu, Xiangtao Kang, Yadong Tian
It has been reported that adiponectin (AdipoQ), an adipokine secreted by white adipose tissue, plays an important role in the control of animal reproduction in addition to its function in energy homeostasis by binding to its receptors AdipoR1/2. However, the molecular mechanisms of AdipoQ in the regulation of animal reproduction remain elusive. In this study, we investigated the effects of AdipoQ on hypothalamic reproductive hormone (GnRH) secretion and reproduction-related receptor gene (estrogen receptor [ER] and progesterone receptor [PR]) expression in hypothalamic neuronal cells (HNCs) of chickens by using real-time fluorescent quantitative PCR (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), Western blot (WB) and cell counting kit-8 (CCK-8) assays and found that overexpression of AdipoQ could increase the expression levels of AdipoR1/2 and reproduction-related receptor genes (P < 0...
August 16, 2023: Poultry Science