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https://www.readbyqxmd.com/read/28719653/isolation-of-a-monoclonal-antibody-from-a-phage-display-library-binding-the-rhesus-macaque-mhc-class-i-allomorph-mamu-a1-001
#1
Nathan Holman, Jason T Weinfurter, Trevor R Harsla, Roger W Wiseman, Aaron J Belli, Anthony J Michaels, Keith A Reimann, Robert I DeMars, Matthew R Reynolds
Monoclonal antibodies that bind to human leukocyte antigen (HLA) are useful tools for HLA-typing, tracking donor-recipient chimerisms after bone marrow transplants, and characterizing specific major histocompatibility complexes (MHC) on cell surfaces. Unfortunately, equivalent reagents are not available for rhesus macaques, which are commonly used animal as models in organ transplant and infectious disease research. To address this deficiency, we isolated an antibody that recognizes the common Indian rhesus macaque MHC class I molecule, Mamu-A1*001...
2017: PloS One
https://www.readbyqxmd.com/read/28719188/phage-selection-of-cyclic-peptides-for-application-in-research-and-drug-development
#2
Kaycie Deyle, Xu-Dong Kong, Christian Heinis
Cyclic peptides can bind to protein targets with high affinities and selectivities, which makes them an attractive modality for the development of research reagents and therapeutics. Additional properties, including low inherent toxicity, efficient chemical synthesis, and facile modification with labels or immobilization reagents, increase their attractiveness. Cyclic peptide ligands against a wide range of protein targets have been isolated from natural sources such as bacteria, fungi, plants, and animals...
July 18, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28715634/a-novel-strategy-utilizing-extracellular-cysteine-rich-domain-of-membrane-receptor-for-constructing-d-peptide-mediated-targeted-drug-delivery-systems-a-case-study-on-fn14
#3
Zhuoxuan Li, Jing Xie, Shan Peng, Sha Liu, Ying Wang, Weiyue Lu, Jie Shen, Chong Li
The development of proteolysis-resistant D-peptide ligands for targeted drug/gene delivery has been greatly limited, due to the challenge lies in the chemical synthesis of membrane receptors without altering their structures. In the present research, a novel strategy utilizing self-stabilized extracellular cysteine-rich domain (CRD) of membrane receptor was developed to construct D-peptide ligands and their mediated targeted drug delivery systems. Fibroblast growth factor-inducible 14 (Fn14), a cell surface receptor overexpressed in many cancers including pancreatic and triple-negative breast cancers, was selected as the model receptor...
July 17, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28715199/jumping-hurdles-peptides-able-to-overcome-biological-barriers
#4
Macarena Sánchez-Navarro, Meritxell Teixidó, Ernest Giralt
The cell membrane, the gastrointestinal tract, and the blood-brain barrier (BBB) are good examples of biological barriers that define and protect cells and organs. They impose different levels of restriction, but they also share common features. For instance, they all display a high lipophilic character. For this reason, hydrophilic compounds, like peptides, proteins, or nucleic acids have long been considered as unable to bypass them. However, the discovery of cell-penetrating peptides (CPPs) opened a vast field of research...
July 17, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28711695/identification-of-a-conformational-neutralizing-epitope-on-the-vp1-protein-of-type-a-foot-and-mouth-disease-virus
#5
Wenming Liu, Baolin Yang, Mingxia Wang, Haiwei Wang, Decheng Yang, Wenge Ma, Guohui Zhou, Li Yu
Foot-and-mouth disease (FMD) caused by foot-and-mouth disease virus (FMDV), is a highly contagious infectious disease that affects domestic and wild cloven-hoofed animals worldwide. In recent years, outbreaks of serotype A FMD have occurred in many countries. High-affinity neutralizing antibodies against a conserved epitope could provide protective immunity against diverse subtypes of FMDV serotype A and protect against future pandemics. In this study, we generated a serotype A FMDV-specific potent neutralizing monoclonal antibody (MAb), 6C9, which recognizes a conformation-dependent epitope...
July 8, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28710679/new-phage-display-isolated-heptapeptide-recognizing-the-regulatory-carboxy-terminal-domain-of-human-tumour-protein-p53
#6
Sihem Ben Abid, Mouna Sahnoun, Ines Yacoubi-Hadj Amor, Salma Abdelmoula-Souissi, Hajer Hassairi, Raja Mokdad-Gargouri, Ali Gargouri
The transcription factor tumor protein p53 (P53) controls a variety of genes most involved in cell cycle and is at the origin of apoptosis when DNA is irreparably damaged. We planned to select novel tumor protein p53-interacting peptides through the screening of hepta-peptide phage-display libraries. For this aim, human tumor suppressor protein p53 was expressed in Escherichia coli as Glutathione S-transferase fusion and purified by affinity chromatography. The phage library was then screened on this immobilized protein target...
July 14, 2017: Protein Journal
https://www.readbyqxmd.com/read/28701874/the-interaction-of-serum-albumin-with-ginsenoside-rh2-resulted-in-the-downregulation-of-ginsenoside-rh2-cytotoxicity
#7
Yingjia Lin, Yang Li, Zhi-Guang Song, Hongyan Zhu, Ying-Hua Jin
BACKGROUND: Ginsenoside Rh2 (G-Rh2) is a ginseng saponin that is widely investigated because of its remarkable antitumor activity. However, the molecular mechanism by which (20S) G-Rh2 triggers its functions and how target animals avoid its cytotoxic action remains largely unknown. METHODS: Phage display was used to screen the human targets of (20S) G-Rh2. Fluorescence spectroscopy and UV-visible absorption spectroscopy were used to confirm the interaction of candidate target proteins and (20S) G-Rh2...
July 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28701344/syp1-regulates-the-clathrin-mediated-and-clathrin-independent-endocytosis-of-multiple-cargo-proteins-through-a-novel-sorting-motif
#8
Amanda Reider Apel, Kyle Hoban, Silvia Chuartzman, Raffi Tonikian, Sachdev Sidhu, Maya Schuldiner, Beverly Wendland, Derek Prosser
Internalization of proteins from the plasma membrane (PM) allows for cell-surface composition regulation, signaling network modulation, and nutrient uptake. Clathrin-mediated endocytosis (CME) is a major internalization route for PM proteins. During CME, endocytic adaptor proteins bind cargos at the cell surface and link them to the PM and clathrin coat. Muniscins are a conserved family of endocytic adaptors, including Syp1 in budding yeast and its mammalian ortholog, FCHo1. These adaptors bind cargo via a C-terminal ì-homology domain (ìHD); however, few cargos exhibiting muniscin-dependent endocytosis have been identified, and the sorting sequence recognized by the μHD is unknown...
July 12, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28690185/selection-of-similar-single-domain-antibodies-from-two-immune-vhh-libraries-obtained-from-two-alpacas-by-using-different-selection-methods
#9
Tengfei Li, Matthias Vandesquille, Sylvie Bay, Marc Dhenain, Benoît Delatour, Pierre Lafaye
The two most used methods to select camelid single-domain antibody-fragments (VHHs) are: displaying their repertoires on the surface of filamentous bacteriophages (phage display) or linking them to ribosomes (ribosome display). In this study, we compared specific VHHs isolated from two different immune libraries coming from two different alpacas by using these two selection methods. Three anti-GFAP (glial fibrillary acidic protein) VHHs were derived from an immune library obtained by ribosome display after immunization of one alpaca with purified GFAP, a protein expressed by astroglial cells...
July 6, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28689690/genome-analysis-of-three-novel-lytic-vibrio-coralliilyticus-phages-isolated-from-seawater-okinawa-japan
#10
Chitra Ramphul, Beatriz Estela Casareto, Hideo Dohra, Tomohiro Suzuki, Katsuhiko Yoshimatsu, Koichi Yoshinaga, Yoshimi Suzuki
Three novel Vibrio phages were isolated from seawater in Okinawa. The Vibrio phage RYC infected Vibrio coralliilyticus SWA 07, while Vibrio phages CKB-S1 and CKB-S2 infected the coral pathogen V. coralliilyticus P1 (LMG 23696). The Vibrio phages CKB-S1 and CKB-S2 displayed head-tail structures whereas the Vibrio phage RYC showed a tailless non-enveloped capsid. All these Vibrio phages contained linear and double-stranded DNA. The whole genome sequencing revealed that Vibrio phage RYC has a larger genome size compared to Vibrio phages CKB-S1 and CKB-S2, and six tRNAs genes were found only in Vibrio phage RYC...
July 6, 2017: Marine Genomics
https://www.readbyqxmd.com/read/28688285/a-strategy-for-actualization-of-active-targeting-nanomedicine-practically-functioning-in-a-living-body
#11
Kyoung Jin Lee, Seol Hwa Shin, Jae Hee Lee, Eun Jin Ju, Yun-Yong Park, Jung Jin Hwang, Young-Ah Suh, Seung-Mo Hong, Se Jin Jang, Jung Shin Lee, Si Yeol Song, Seong-Yun Jeong, Eun Kyung Choi
Designing nanocarriers with active targeting has been increasingly emphasized as for an ideal delivery mechanism of anti-cancer therapeutic agents, but the actualization has been constrained by lack of reliable strategy ultimately applicable. Here, we designed and verified a strategy to achieve active targeting nanomedicine that works in a living body, utilizing animal models bearing a patient's tumor tissue and subjected to the same treatments that would be used in the clinic. The concept for this strategy was that a novel peptide probe and its counterpart protein, which responded to a therapy, were identified, and then the inherent ability of the peptide to target the designated tumor protein was used for active targeting in vivo...
June 30, 2017: Biomaterials
https://www.readbyqxmd.com/read/28683141/isolation-of-serotype-specific-antibodies-against-dengue-virus-non-structural-protein-1-using-phage-display-and-application-in-a-multiplexed-serotyping-assay
#12
Kebaneilwe Lebani, Martina L Jones, Daniel Watterson, Andrea Ranzoni, Renee J Traves, Paul R Young, Stephen M Mahler
The multidimensional nature of dengue virus (DENV) infections, which can be caused by four distinct serotypes of the virus, complicates the sensitivity of assays designed for the diagnosis of infection. Different viral markers can be optimally detected at different stages of infection. Of particular clinical importance is the early identification of infection, which is pivotal for disease management and the development of blood screening assays. Non-structural protein 1 (NS1) is an early surrogate marker of infection and its detection in serum coincides with detectable viraemia...
2017: PloS One
https://www.readbyqxmd.com/read/28676225/identification-of-cytidine-5-triphosphate-synthase1-selective-inhibitory-peptide-from-random-peptide-library-displayed-on-t7-phage
#13
Kotaro Sakamoto, Yoshihiro Ishibashi, Ryutaro Adachi, Shin-Ichi Matsumoto, Hideyuki Oki, Yusuke Kamada, Satoshi Sogabe, Yumi Zama, Jun-Ichi Sakamoto, Akiyoshi Tani
Cytidine triphosphate synthase 1 (CTPS1) is an enzyme expressed in activated lymphocytes that catalyzes the conversion of uridine triphosphate (UTP) to cytidine triphosphate (CTP) with ATP-dependent amination, using either L-glutamine or ammonia as the nitrogen source. Since CTP plays an important role in DNA/RNA synthesis, phospholipid synthesis, and protein sialyation, CTPS1-inhibition is expected to control lymphocyte proliferation and size expansion in inflammatory diseases. In contrast, CTPS2, an isozyme of CTPS1 possessing 74% amino acid sequence homology, is expressed in normal lymphocytes...
July 1, 2017: Peptides
https://www.readbyqxmd.com/read/28669053/efficient-targeting-of-cd13-on-cancer-cells-by-the-immunotoxin-scfv13-eta-and-the-bispecific-scfv-13xds16
#14
Elena Grieger, Gerrit Gresch, Judith Niesen, Mira Woitok, Stefan Barth, Rainer Fischer, Rolf Fendel, Christoph Stein
PURPOSE: Treatment of cancer using standard chemotherapy still offers a poor prognosis combined with severe side effects. Novel antibody-based therapies have been shown to overcome low efficiency and lack of selectivity by targeting cancer-associated antigens, such as aminopeptidase CD13. METHODS: We isolated a high-affinity CD13-specific single-chain fragment variable (scFv13) from a phage display library of V-genes from mice immunized with soluble antigen. An immunotoxin comprising the scFv13 and a truncated version of the exotoxin A of Pseudomonas aeruginosa (ETA', scFv13-ETA') and a bispecific scFv targeting CD13 and CD16 simultaneously (bsscFv[13xds16]) was generated and investigated for their therapeutic potential...
July 1, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28667526/isolation-of-natural-anti-fc%C3%AE%C2%B5ri%C3%AE-autoantibodies-from-healthy-donors
#15
Monique Vogel, Michael P Horn
Natural antibodies are defined as antibodies detected in a healthy individual without active immunization. These antibodies are specific for exoantigens, as well as for autoantigens, mostly without any pathogenic role. Most of the studies conducted with natural (auto-) antibodies have been performed using affinity purified antibodies from individual sera or polyclonal Ig-preparations such as Intravenous Ig (IVIg). For in-depth analysis of such autoantibodies affinity-purified Ig-preparations from healthy individuals are of no use, as they are oligoclonal or polyclonal...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28664268/biased-selection-of-propagation-related-tups-from-phage-display-peptide-libraries
#16
REVIEW
Hesam Motaleb Zade, Reihaneh Keshavarz, Hosna Sadat Zahed Shekarabi, Babak Bakhshinejad
Phage display is rapidly advancing as a screening strategy in drug discovery and drug delivery. Phage-encoded combinatorial peptide libraries can be screened through the affinity selection procedure of biopanning to find pharmaceutically relevant cell-specific ligands. However, the unwanted enrichment of target-unrelated peptides (TUPs) with no true affinity for the target presents an important barrier to the successful screening of phage display libraries. Propagation-related TUPs (Pr-TUPs) are an emerging but less-studied category of phage display-derived false-positive hits that are displayed on the surface of clones with faster propagation rates...
June 29, 2017: Amino Acids
https://www.readbyqxmd.com/read/28661364/recombinant-antibodies-for-academia-a-practical-approach
#17
Pierre Cosson, Oliver Hartley
After several decades of optimization, phage display technology enables the routine isolation and production of recombinant monoclonal antibodies in vitro. As such it has the potential to provide the academic community with a vast, inexpensive and renewable supply of well-characterized reagents, reducing bottlenecks in basic science, helping increase reproducibility of experiments, and phasing out the use of animals for production and discovery of antibodies. Yet the overwhelming majority of fundamental research laboratories still use incompletely characterized antibodies developed in animals...
December 21, 2016: Chimia
https://www.readbyqxmd.com/read/28659884/fungal-glucosylceramide-specific-camelid-single-domain-antibodies-are-characterized-by-broad-spectrum-antifungal-activity
#18
Barbara De Coninck, Peter Verheesen, Christine M Vos, Inge Van Daele, Miguel F De Bolle, Joao V Vieira, Marnix Peferoen, Bruno P A Cammue, Karin Thevissen
Chemical crop protection is widely used to control plant diseases. However, the adverse effects of pesticide use on human health and environment, resistance development and the impact of regulatory requirements on the crop protection market urges the agrochemical industry to explore innovative and alternative approaches. In that context, we demonstrate here the potential of camelid single domain antibodies (VHHs) generated against fungal glucosylceramides (fGlcCer), important pathogenicity factors. To this end, llamas were immunized with purified fGlcCer and a mixture of mycelium and spores of the fungus Botrytis cinerea, one of the most important plant pathogenic fungi...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28659622/determination-of-aspergillus-pathogens-in-agricultural-products-by-a-specific-nanobody-polyclonal-antibody-sandwich-elisa
#19
Ting Wang, Peiwu Li, Qi Zhang, Wen Zhang, Zhaowei Zhang, Tong Wang, Ting He
Aspergillus and its poisonous mycotoxins are distributed worldwide throughout the environment and are of particular interest in agriculture and food safety. In order to develop a specific method for rapid detection of Aspergillus flavus to forecast diseases and control aflatoxins, a nanobody, PO8-VHH, highly reactive to A. flavus was isolated from an immunized alpaca nanobody library by phage display. The nanobody was verified to bind to the components of extracellular and intracellular antigen from both A...
June 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28658990/optimization-of-peptides-that-actively-cross-the-tympanic-membrane-by-random-amino-acid-extension-a-phage-display-study
#20
Arwa Kurabi, Daniel Schaerer, Lisa Chang, Kwang Pak, Allen F Ryan
Local treatment of middle ear (ME) disease currently requires surgical penetration of the tympanic membrane (TM). We previously discovered 12-mer peptides that are actively transported across the intact TM, a process that could be used for noninvasive drug delivery into the ME. To optimize transport and provide further understanding of the peptides transport mechanism, we extended two of the candidate peptides by six additional amino acids at random, and screened the resulting 18-mers libraries on TMs of rats with active bacterial otitis media (OM) for transport efficiency using phage display...
June 29, 2017: Journal of Drug Targeting
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