keyword
MENU ▼
Read by QxMD icon Read
search

Ferment

keyword
https://www.readbyqxmd.com/read/28330332/response-surface-methodology-based-optimization-of-production-media-and-purification-of-%C3%AE-galactosidase-in-solid-state-fermentation-by-fusarium-moniliforme-ncim-1099
#1
Sanjivani B Gajdhane, Prashant K Bhagwat, Padma B Dandge
Response surface methodology was used to enhance the production of α-galactosidase from Fusarium moniliforme NCIM 1099 in solid-state fermentation. Plackett-Burman design was employed for selection of critical media constituents which were optimized by central composite rotatable design. Wheat bran, peptone and FeSO4·7H2O were identified as significant medium components using PB design. Further CCRD optimized medium components as wheat bran; 4.62 μg, peptone; 315.42 μg, FeSO4·7H2O; 9.04 μg. RSM methodological optimization increased the enzyme production from 13...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330328/optimization-of-phytase-production-from-potato-waste-using-aspergillus-ficuum
#2
Mengmeng Tian, Qiuyan Yuan
Solid-state fermentation (SSF) can divert food waste from landfills and produce high-value products. This study was aimed to investigate the feasibility of using SSF and optimize the conditions of production of phytase by Aspergillus ficuum from potato waste. Different parameters including pH of the potato waste, inoculum level, moisture content, incubation period, temperature, and supplementary nitrogen and carbon sources were evaluated. The results indicated that pH, inoculum level, and moisture content did not significantly vary phytase production...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330311/scaling-up-a-virginiamycin-production-by-a-high-yield-streptomyces-virginiae-vkm-ac-2738d-strain-using-adsorbing-resin-addition-and-fed-batch-fermentation-under-controlled-conditions
#3
Vakhtang Dzhavakhiya, Vyacheslav Savushkin, Alexander Ovchinnikov, Vladislav Glagolev, Veronika Savelyeva, Evgeniya Popova, Nikita Novak, Elena Glagoleva
Virginiamycin produced by Streptomyces virginiae as a natural mix of macrocyclic peptidolactones M and S is widely used in the industrial production of ethanol fuel and as an antibiotic feed additive for cattle and poultry. Its main antimicrobial components, M1 and S1 factors, act synergistically if the M1:S1 ratio in the final product is 70-75:25-30. This fact significantly complicates the development of stable high-yield strains suitable for industrial application. In the previous work, authors obtained a mutant S...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330308/construction-of-aspergillus-niger-integrated-with-cellulase-gene-from-ampullaria-gigas-spix-for-improved-enzyme-production-and-saccharification-of-alkaline-pretreated-rice-straw
#4
Peizhou Yang, Haifeng Zhang, Lili Cao, Zhi Zheng, Shaotong Jiang
Aspergillus niger is an important microorganism that has been used for decades to produce extracellular enzymes. In this study, a novel Aspergillus niger strain integrated with a eukaryotic expression vector harboring the gpd-Shi promoter of shiitake mushrooms and cellulase gene of Ampullaria gigas Spix was engineered to improve cellulase production for the achievement of highly efficient saccharification of agricultural residues. In one strain, designated ACShi27, which exhibited the highest total cellulase expression, total cellulase, endoglucanase, exoglucanase, and xylanase expression levels were 1...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330307/effect-of-particle-size-moisture-content-and-supplements-on-selective-pretreatment-of-cotton-stalks-by-daedalea-flavida-and-enzymatic-saccharification
#5
Harmanpreet Meehnian, Asim K Jana, Mithu Maiti Jana
A large amount of cotton stalk waste biomass with high cellulose content are incinerated by the farmers causing air pollution. The high cellulose content of cotton stalks can be converted to fermentable sugars by fungal delignification pretreatment of lignocellulosic biomass and enzymatic saccharification. The effect of particle size, moisture content, and media supplements was studied for delignification of cotton stalks by Daedalea flavida MTCC 145 (DF-2) in solid-state fermentation. The highest lignolytic enzyme activities, optimal lignin degradation 29...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330303/bio-prospecting-of-cuttle-fish-waste-and-cow-dung-for-the-production-of-fibrinolytic-enzyme-from-bacillus-cereus-ind5-in-solid-state-fermentation
#6
Gurupatham Devadhasan Biji, Arumugaperumal Arun, Eswaran Muthulakshmi, Ponnuswamy Vijayaraghavan, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi
The process parameters governing the production of fibrinolytic enzyme in solid state fermentation employing Bacillus cereus IND5 and using cuttle fish waste and cow dung substrate were optimized. The pH value of the medium, moisture content, sucrose, casein and magnesium sulfate were considered for two-level full factorial design and pH, casein and magnesium sulfate were identified as the important factors for fibrinolytic enzyme production. Central composite design was applied to investigate the interactive effect among variables (pH, casein and magnesium sulfate) and response surface plots were created to find the pinnacle of process response...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330302/production-of-bioherbicide-by-phoma-sp-in-a-stirred-tank-bioreactor
#7
Thiarles Brun, Jéssica E Rabuske, Izelmar Todero, Thiago C Almeida, Jair J D Junior, Gustavo Ariotti, Tássia Confortin, Jonas A Arnemann, Raquel C Kuhn, Jerson V C Guedes, Marcio A Mazutti
The objective of this work was to produce an herbicide by submerged fermentation in a stirred-tank bioreactor and to assess the potential herbicidal in pre-emergence, post-emergence, and in a detached leaves of Cucumis sativus var species. wisconsin (cucumber) and Sorghum bicolor (sorghum) species. Fermentations were carried out in a stirred-tank bioreactor with useful volume of 3L. Stirring rate (40, 50, and 60 rpm) and aeration (1, 2 and 3 vvm) were the variables studied for bioherbicide production. Fermented broth was fractioned with different solvents to identify the molecules produced by the fungus in a multi-dimensional gas chromatograph system...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330295/biomass-derived-molecules-modulate-the-behavior-of-streptomyces-coelicolor-for-antibiotic-production
#8
Shashi Kant Bhatia, Bo-Rahm Lee, Ganesan Sathiyanarayanan, Hun Seok Song, Junyoung Kim, Jong-Min Jeon, Jeong-Jun Yoon, Jungoh Ahn, Kyungmoon Park, Yung-Hun Yang
Various chemicals, i.e., furfural, vanillin, 4-hydroxybenzaldehyde and acetate produced during the pretreatment of biomass affect microbial fermentation. In this study, effect of vanillin, 4-hydroxybenzaldehyde and acetate on antibiotic production in Streptomyces coelicolor is investigated. IC 50 value of vanillin, 4-hydroxybenzaldehyde and acetate was recorded as 5, 11.3 and 115 mM, respectively. Vanillin was found as a very effective molecule, and it completely abolished antibiotic (undecylprodigiosin and actinorhodin) production at 1 mM concentration, while 4-hydroxybenzaldehyde and acetate have little effect...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330286/effect-of-highly-branched-hyphal-morphology-on-the-enhanced-production-of-cellulase-in-trichoderma-reesei-des-15
#9
Ronglin He, Chen Li, Lijuan Ma, Dongyuan Zhang, Shulin Chen
The morphology of Trichoderma reesei is a vitally important factor for cellulase productivity. This study investigated the effect of hyphal morphology on cellulase production in the hyper-cellulolytic mutant, T. reesei DES-15. With a distinct morphology, T. reesei DES-15 was obtained through Diethyl sulfite (DES) mutagenesis. The hyphal morphology of DES-15 batch-cultured in a 5-L fermentor was significantly shorter and more branched than the parental strain RUT C30. The cellulase production of DES-15 during batch fermentation was 66 % greater than that of RUT C30 when cultured the same conditions...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330279/immobilization-of%C3%A2-bacillus-amyloliquefaciens-sp1-and-its-alkaline-protease-in-various-matrices-for-effective-hydrolysis-of-casein
#10
Shiwani Guleria, Abhishek Walia, Anjali Chauhan, C K Shirkot
An extracellular alkaline protease producing B. amyloliquefaciens SP1 was isolated from apple rhizosphere having multifarious plant growth-promoting activities. B. amyloliquefaciens SP1 protease was immobilized using various concentrations of calcium alginate, agar and polyacrylamide to determine the optimum concentration for formation of the beads. Enzyme activity before immobilization (at 60 °C, pH 8.0 for 5 min) was 3580 µg/ml/min. The results of immobilization with various matrices revealed that 3 % calcium alginate (2829...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330278/effects-of-the-culture-media-optimization-on-pectinase-production-by-enterobacter-sp-pstb-1
#11
M Purna Chandra Reddy, K V Saritha
In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330274/evaluation-of-some-lignocellulosic-byproducts-of-food-industry-for-microbial-xylitol-production-by-candida-tropicalis
#12
Kubra Eryasar, Seda Karasu-Yalcin
Some lignocellulosic food byproducts such as potato peels, wheat bran, barley bran and chestnut shells were evaluated as potential sources of xylose for microbial xylitol production by yeasts. Potential yeast strains were selected after screening xylitol production of some indigenous yeasts in a defined fermentation medium. Candida tropicalis strains gave the highest results with 83.28 and 54.07 g/L xylitol production from 100 g/L xylose. Lignocellulosic materials were exposed to acid hydrolysis at different conditions...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330273/purification-and-characterization-of-polygalacturonase-from-aspergillus-fumigatus-mtcc-2584-and-elucidating-its-application-in-retting-of-crotalaria-juncea-fiber
#13
Gautam Anand, Sangeeta Yadav, Dinesh Yadav
Polygalacturonases represents an important member of pectinases group of enzymes with diverse industrial applications and is widely distributed among fungi, bacteria, yeasts, plants and some plant parasitic nematodes. An endo-polygalacturonase from a new fungal source Aspergillus fumigatus MTCC 2584 was produced under solid-state fermentation conditions and was purified simply by acetone precipitation and gel-filtration chromatography technique. The approximate molecular weight of the purified PG was found to be 43...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330264/construction-of-recombinant-sestc-saccharomyces-cerevisiae-for-consolidated-bioprocessing-cellulase-characterization-and-ethanol-production-by-in-situ-fermentation
#14
Peizhou Yang, Haifeng Zhang, Shaotong Jiang
Bioethanol is an important oil substitute produced by the sugar fermentation process. To improve the efficiency of cellulase expression of Saccharomyces cerevisiae, a eukaryotic expression vector harboring a single-enzyme-system-three-cellulase gene (sestc) was integrated into the S. cerevisiae genome by the protoplast method. Using PCR screening, RT-PCR, and "transparent circle" detection, several recombinant S. cerevisiae strains, capable of efficiently expressing the heterogeneous cellulase, were selected...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330258/optimization-of-d-lactic-acid-production-using-unutilized-biomass-as-substrates-by-multiple-parallel-fermentation
#15
Elya Mufidah, Mamoru Wakayama
This study investigated the optimization of D-lactic acid production from unutilized biomass, specifically banana peel and corncob by multiple parallel fermentation (MPF) with Leuconostoc mesenteroides and Aspergillus awamori. The factors involved in MPF that were assessed in this study comprised banana peel and corncob, KH2PO4, Tween 80, MgSO4·7H2O, NaCl, yeast extract, and diammonium hydrogen citrate to identify the optimal concentration for D-lactic acid production. Optimization of these component factors was performed using the Taguchi method with an L8 orthogonal array...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330237/biocatalytic-activity-of-aspergillus-niger-xylanase-in-paper-pulp-biobleaching
#16
A Sridevi, A Sandhya, G Ramanjaneyulu, G Narasimha, P Suvarnalatha Devi
Xylanase is a hemicellulase enzyme that catalyses the hydrolysis of xylan to xylose which is widely used in processing of feed, pulp and paper. It is produced by many microorganisms especially filamentous fungi like Trichoderma and Aspergillus. A potential xylanolytic fungal isolate Aspergillus niger was isolated from forest soils of Tirumala, AP, India, and its crude enzyme was checked for its potential in paper bleaching. Under submerged fermentation, production of xylanase, cellulase, biomass, total protein and sugar released were analysed after 7 days of incubation at room temperature...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330236/xylanase-production-from-penicillium-citrinum-isolate-hzn13-using-response-surface-methodology-and-characterization-of-immobilized-xylanase-on-glutaraldehyde-activated-calcium-alginate-beads
#17
Zabin K Bagewadi, Sikandar I Mulla, Yogesh Shouche, Harichandra Z Ninnekar
The present study reports the production of high-level cellulase-free xylanase from Penicillium citrinum isolate HZN13. The variability in xylanase titers was assessed under both solid-state (SSF) and submerged (SmF) fermentation. SSF was initially optimized with different agro-waste residues, among them sweet sorghum bagasse was found to be the best substrate that favored maximum xylanase production (9643 U/g). Plackett-Burman and response surface methodology employing central composite design were used to optimize the process parameters for the production of xylanase under SSF...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330225/metagenomics-analysis-of-microbial-communities-associated-with-a-traditional-rice-wine-starter-culture-xaj-pitha-of-assam-india
#18
Sudipta Sankar Bora, Jyotshna Keot, Saurav Das, Kishore Sarma, Madhumita Barooah
This is the first report on the microbial diversity of xaj-pitha, a rice wine fermentation starter culture through a metagenomics approach involving Illumine-based whole genome shotgun (WGS) sequencing method. Metagenomic DNA was extracted from rice wine starter culture concocted by Ahom community of Assam and analyzed using a MiSeq(®) System. A total of 2,78,231 contigs, with an average read length of 640.13 bp, were obtained. Data obtained from the use of several taxonomic profiling tools were compared with previously reported microbial diversity studies through the culture-dependent and culture-independent method...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330223/multi-response-optimization-for-enhanced-xylitol-production-by-debaryomyces-nepalensis-in-bioreactor
#19
J Sharon Mano Pappu, Sathyanarayana N Gummadi
In this study, the optimization of different process variables-pH (4-6), aeration rate (200-550 rpm) and agitation rate (0.6-1.8 vvm) were investigated using rotating simplex method and uniform design method to enhance xylitol production from xylose by D. nepalensis in a batch stirred tank bioreactor. Maximum xylitol productivity (0.576 g L(-1) h(-1)) was obtained at pH 4.0, agitation 300 rpm and aeration 1.5 vvm by rotating simplex method. Individual optimum values of pH, agitation and aeration are 4...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330216/potential-application-of-waste-from-castor-bean-ricinus-communis-l-for-production-for-xylanase-of-interest-in-the-industry
#20
Polyanna Nunes Herculano, Keila Aparecida Moreira, Raquel Pedrosa Bezerra, Tatiana Souza Porto, Cristina Maria de Souza-Motta, Ana Lúcia Figueiredo Porto
Xylanases activity (XY) from Aspergillus japonicus URM5620 produced by Solid-State Fermentation (SSF) of castor press cake (Ricinus communis) on different conditions of production and extraction by PEG/citrate aqueous two-phase system (ATPS) were investigated. XY production was influenced by substrate amount (5-10 g), initial moisture (15-35 %), pH (4.0-6.0) and temperature (25-35 °C), obtaining the maximum activity of 29,085 ± 1808 U g ds(-1) using 5.0 g of substrate with initial moisture of 15 % at 25 °C and pH 6...
December 2016: 3 Biotech
keyword
keyword
34409
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"