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https://www.readbyqxmd.com/read/29161679/the-changes-of-nutrient-composition-of-piled-laying-hen-manure-and-anaerobic-fermentation-for-recycling-as-a-dietary-ingredient-for-ruminants
#1
Tianlong Han, Liping Wang, Yanming Zhang, Jun Zhang, Dongsheng Han, Ning Lv, Xiaohua Han, Guoqiang Zhao, Min Wang
This study investigated the changes of nutrient compositions of piled laying hen manure, detected the physical and chemical components of laying hen manure fermented by mixed strains, and analysed its application effects on feeding ruminants. The results showed that with increasing of piling time, the contents of crude protein (CP) and ether extract (EE) in laying hen manure were reduced, while the contents of crude ash (CA), calcium (Ca) and phosphorus (P) were increased. Fermentation could effectively decrease pH value, reduce Ca/P ratio, lessen the total bacterial counts, and maintain the organisms contents in laying hen manure...
November 18, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29161574/fast-spectroscopic-monitoring-of-inhibitors-in-the-2g-ethanol-process
#2
Ariane S S Pinto, Marcelo P A Ribeiro, Cristiane S Farinas
One of the main challenges of second generation (2G) ethanol production is the high quantities of phenolic compounds and furan derivatives generated in the pretreatment of the lignocellulosic biomass, which inhibit the enzymatic hydrolysis and fermentation steps. Fast monitoring of these inhibitory compounds could provide better control of the pretreatment, hydrolysis, and fermentation processes by enabling the implementation of strategic process control actions. We investigated the feasibility of monitoring these inhibitory compounds by ultraviolet-visible (UV-Vis) spectroscopy associated with partial least squares (PLS) regression...
November 14, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29161572/new-%C3%AE-galactosidase-producers-with-potential-for-prebiotic-synthesis
#3
Sara C Silvério, Eugénia A Macedo, José A Teixeira, Lígia R Rodrigues
β-Galactosidases (EC 3.2.1.23) are interesting enzymes with potential application in the pharmaceutical and food industry. In this work, a screening study was carried out to identify new fungal sources of β-galactosidase. A total of 50 fungi were evaluated using a chromogenic test performed in agar plates. The most promising microorganisms were validated as effective β-galactosidase producers under submerged fermentation conditions. The crude β-galactosidases were characterized regarding their optimal pH (3...
November 15, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29161570/acclimation-of-acid-tolerant-methanogenic-propionate-utilizing-culture-and-microbial-community-dissecting
#4
Ying Li, Yongming Sun, Lianhua Li, Zhenhong Yuan
The acid-tolerant methanogenic propionate degradation culture was acclimated in a propionate-fed semi-continuous bioreactor by daily adjusting the digestate pH. The performance of propionate fermentation, the respond of microbial community structure to the acidic environment, and the microbial network for propionate degradation in the acid-tolerant culture was investigated. The results demonstrated that after long term of acclimation to low pH, the digester could produce methane from propionate at pH 4.8-5...
November 13, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29161568/feasibility-of-high-concentration-cellulosic-bioethanol-production-from-undetoxified-whole-monterey-pine-slurry
#5
Chengyu Dong, Ying Wang, Han Zhang, Shao-Yuan Leu
The economic feasibility of high-concentration cellulosic bioethanol production remains challenging because it requires easily available feedstock and low energy consumption process. Simultaneous saccharification and fermentation (SSF) of sulfite pretreated Momentary pine slurry at 20% (w/w) loadings increased ethanol concentration from 59.3 g/L to 68.5 g/L by washing strategy. Effects of inhibitors in pretreatment liquor were further investigated. Besides HMF, furfural and acetic acid, other inhibitors and/or their synergistic effects proved to be responsible for a lower fermentability...
November 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29161457/the-soluble-guanylate-cyclase-cyg12-is-required-for-the-acclimation-to-hypoxia-and-trophic-regimes-in-chlamydomonas-reinhardtii
#6
Melis Düner, Jan Lambertz, Carolin Mügge, Anja Hemschemeier
Oxygenic phototrophs frequently encounter environmental conditions that result in intracellular energy crises. Growth of the unicellular green alga Chlamydomonas reinhardtii in hypoxia in the light depends on acclimatory responses of which the induction of photosynthetic cyclic electron flow is essential. The microalga cannot grow in the absence of molecular oxygen (O2 ) in the dark, although it possesses an elaborate fermentation metabolism. Not much is known about how the microalga senses and signals the lack of O2 or about its survival strategies during energy crises...
November 21, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29161087/the-hygiene-hypothesis-in-the-age-of-the-microbiome
#7
Markus J Ege
The original version of the hygiene hypothesis suggested that infections transmitted early in life by "unhygienic contact" prevented allergies. Examples were endemic fecal-oral infections by viral, bacterial, or protozoic pathogens, such as hepatitis A virus, Helicobacter pylori, or Toxoplasma gondii. Later, this concept also included microorganisms beyond pathogens, such as commensals and symbionts, and the hygiene hypothesis was extended to inflammatory diseases in general. An impressive illustration of the hygiene hypothesis was found in the consistent farm effect on asthma and allergies, which has partly been attributed to immunomodulatory properties of endotoxin as emitted by livestock...
November 2017: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/29160816/optimization-of-goat-milk-with-ace-inhibitory-peptides-fermented-by-lactobacillus-bulgaricus-lb6-using-response-surface-methodology
#8
Guowei Shu, Xiaoyu Shi, He Chen, Zhe Ji, Jiangpeng Meng
In the present study, the incubation conditions of goat milk fermented by Lactobacillus bulgaricus LB6 were optimized to increase the angiotensin converting enzyme (ACE, EC 3.4.15.1) inhibitory activity by Box-Behnken design of response surface methodology. Incubation temperature, whey powder, and calcium lactate had significant effects on ACE inhibition rate and viable counts of LB6 during incubation. The results showed that optimal conditions of fermentation were found to be 37.05 °C, 0.8% (w/w) whey powder and 0...
November 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29160034/free-living-chemoautotrophic-and-particle-attached-heterotrophic-prokaryotes-dominate-microbial-assemblages-along-a-pelagic-redox-gradient
#9
Elizabeth A Suter, Maria Pachiadaki, Gordon T Taylor, Yrene Astor, Virginia P Edgcomb
Using the anoxic Cariaco Basin as a natural laboratory, particle association of bacterial and archaeal taxa was assessed by iTag sequencing and qPCR gene assays of samples spanning an oxic-anoxic-euxinic gradient. Ten to twelve percent of all bacterial and archaeal cells were found in the particle-associated (PA) fraction, operationally defined as prokaryotes captured on 2.7µm membranes. Both redox condition and size fraction segregated bacterial taxa. Archaeal taxa varied according to redox conditions, but were similar between size fractions...
November 21, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/29160026/enrichment-of-syngas-converting-communities-from-a-multi-orifice-baffled-bioreactor
#10
Ana L Arantes, Joana I Alves, Alfons J M Stams, M Madalena Alves, Diana Z Sousa
The substitution of natural gas by renewable biomethane is an interesting option to reduce global carbon footprint. Syngas fermentation has potential in this context, as a diverse range of low-biodegradable materials that can be used. In this study, anaerobic sludge acclimatized to syngas in a multi-orifice baffled bioreactor (MOBB) was used to start enrichments with CO. The main goals were to identify the key players in CO conversion and evaluate potential interspecies metabolic interactions conferring robustness to the process...
November 21, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/29159993/low-fodmaps-diet-vs-general-dietary-advice-improves-clinical-response-in-patients-with-diarrhea-predominant-irritable-bowel-syndrome-a-randomized-controlled-trial
#11
Mohammad Javad Zahedi, Vahideh Behrouz, Maryam Azimi
BACKGROUND AND AIMS: Recent evidence indicates that new approach of the diet with low Fermentable Oligo-Di-Mono-saccharides and polyols (FODMAPs) may has an effective role in management of the patients with irritable bowel syndrome (IBS). We compared the results of low FODMAP diet vs. current dietary treatment, general dietary advices (GDA), on the clinical response in patients with diarrhea subtype of IBS (IBS-D). METHODS: In this randomized, controlled, single-blind trial, we included 110 patients with IBS-D in 2 intervention groups...
November 20, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29159445/probiotic-properties-and-cellular-antioxidant-activity-of-lactobacillus-plantarum-ma2-isolated-from-tibetan-kefir-grains
#12
Wei Tang, Chao Li, Zengguo He, Fen Pan, Shuo Pan, Yanping Wang
Lactobacillus plantarum MA2 was isolated from traditional Chinese Tibetan kefir grains. Its antioxidant properties had been demonstrated in vitro and in vivo previously. In the present study, the probiotic characteristics of this strain were further evaluated by investigating its acid and bile salt tolerances, cell surface hydrophobicity, and autoaggregation, respectively. In addition, the cellular antioxidant activity (CAA) assay was applied to test the antioxidant capacity of the isolate in different growth phases...
November 20, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29159315/l-arginine-biosensors-a-comprehensive-review
#13
REVIEW
Neelam Verma, Ashish Kumar Singh, Minni Singh
Arginine has been considered as the most potent nutraceutics discovered ever, due to its powerful healing property, and it's been known to scientists as the Miracle Molecule. Arginine detection in fermented food products is necessary because, high level of arginine in foods forms ethyl carbamate (EC) during the fermentation process. Therefore, L-arginine detection in fermented food products is very important as a control measure for quality of fermented foods, food supplements and beverages including wine. In clinical analysis arginine detection is important due to their enormous inherent versatility in various metabolic pathways, topmost in the synthesis of Nitric oxide (NO) and tumor growth...
December 2017: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/29159147/osmotic-conditions-could-promote-scfv-antibody-production-in-the-escherichia-coli-hb2151
#14
Ali Mesgari-Shadi, Mohammad Hossein Sarrafzadeh
Introduction: Single chain variable fragment (scFv) antibodies are reduced forms of the whole antibodies that could be regarded as an alternative tool for diagnostic and therapeutic purposes. The optimization of processes and environmental conditions is necessary to increase the production yields and enhance the productivity. This can result in a cost-effective process and respond to the high demand for these antibodies. Methods: In this research, physical and chemical factors influencing the batch fermentation was investigated in 50 mL batch tubes using minimum media to find the optimum conditions for production of a single chain variable fragment antibody in the Escherichia coli HB2151...
2017: BioImpacts: BI
https://www.readbyqxmd.com/read/29158977/the-sensitivity-of-the-yeast-saccharomyces-cerevisiae-to-acetic-acid-is-influenced-by-dom34-and-rpl36a
#15
Bahram Samanfar, Kristina Shostak, Houman Moteshareie, Maryam Hajikarimlou, Sarah Shaikho, Katayoun Omidi, Mohsen Hooshyar, Daniel Burnside, Imelda Galván Márquez, Tom Kazmirchuk, Thet Naing, Paula Ludovico, Anna York-Lyon, Kama Szereszewski, Cindy Leung, Jennifer Yixin Jin, Rami Megarbane, Myron L Smith, Mohan Babu, Martin Holcik, Ashkan Golshani
The presence of acetic acid during industrial alcohol fermentation reduces the yield of fermentation by imposing additional stress on the yeast cells. The biology of cellular responses to stress has been a subject of vigorous investigations. Although much has been learned, details of some of these responses remain poorly understood. Members of heat shock chaperone HSP proteins have been linked to acetic acid and heat shock stress responses in yeast. Both acetic acid and heat shock have been identified to trigger different cellular responses including reduction of global protein synthesis and induction of programmed cell death...
2017: PeerJ
https://www.readbyqxmd.com/read/29158969/5-fluorouracil-and-irinotecan-sn-38-have-limited-impact-on-colon-microbial-functionality-and-composition-in-vitro
#16
Eline Vanlancker, Barbara Vanhoecke, Andrea Stringer, Tom Van de Wiele
Gastrointestinal mucositis is a debilitating side effect of chemotherapy treatment, with currently no treatment available. As changes in microbial composition have been reported upon chemotherapy treatment in vivo, it is thought that gut microbiota dysbiosis contribute to the mucositis etiology. Yet it is not known whether chemotherapeutics directly cause microbial dysbiosis, thereby increasing mucositis risk, or whether the chemotherapeutic subjected host environment disturbs the microbiome thereby aggravating the disease...
2017: PeerJ
https://www.readbyqxmd.com/read/29158776/genomics-and-prevalence-of-bacterial-and-archaeal-isolates-from-biogas-producing-microbiomes
#17
Irena Maus, Andreas Bremges, Yvonne Stolze, Sarah Hahnke, Katharina G Cibis, Daniela E Koeck, Yong S Kim, Jana Kreubel, Julia Hassa, Daniel Wibberg, Aaron Weimann, Sandra Off, Robbin Stantscheff, Vladimir V Zverlov, Wolfgang H Schwarz, Helmut König, Wolfgang Liebl, Paul Scherer, Alice C McHardy, Alexander Sczyrba, Michael Klocke, Alfred Pühler, Andreas Schlüter
Background: To elucidate biogas microbial communities and processes, the application of high-throughput DNA analysis approaches is becoming increasingly important. Unfortunately, generated data can only partialy be interpreted rudimentary since databases lack reference sequences. Results: Novel cellulolytic, hydrolytic, and acidogenic/acetogenic Bacteria as well as methanogenic Archaea originating from different anaerobic digestion communities were analyzed on the genomic level to assess their role in biomass decomposition and biogas production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29158640/a-sucrose-non-fermenting-1-related-protein-kinase-1-gene-from-potato-stsnrk1-regulates-carbohydrate-metabolism-in-transgenic-tobacco
#18
Feibing Wang, Yuxiu Ye, Xinhong Chen, Jizhong Wang, Zhiyuan Chen, Qing Zhou
Sucrose non-fermenting-1-related protein kinase 1 (SnRK1) has been shown to play an essential role in regulating saccharide metabolism and starch biosynthesis of plant. The regulatory role of StSnRK1 from potato in regulating carbohydrate metabolism and starch accumulation has not been investigated. In this work, a cDNA encoding the SnRK1 protein, named StSnRK1, was isolated from potato. The open reading frame contained 1545 nucleotides encoding 514 amino acids. Subcellular localization analysis in onion epidermal cells indicated that StSnRK1 protein was localized to the nucleus...
October 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29158587/analysis-of-the-gut-microbiome-and-plasma-short-chain-fatty-acid-profiles-in-a-spontaneous-mouse-model-of-metabolic-syndrome
#19
Kazuchika Nishitsuji, Jinzhong Xiao, Ryosuke Nagatomo, Hitomi Umemoto, Yuki Morimoto, Hiroyasu Akatsu, Koichi Inoue, Koichi Tsuneyama
Male Tsumura Suzuki obese diabetes (TSOD) mice spontaneously develop obesity and obesity-related metabolic syndrome. Gut dysbiosis, an imbalance of gut microbiota, has been implicated in the pathogenesis of metabolic syndrome, but its mechanisms are unknown. Short-chain fatty acids (SCFAs) are the main fermentation products of gut microbiota and a link between the gut microbiota and the host's physiology. Here, we investigated a correlation among gut dysbiosis, SCFAs, and metabolic syndrome in TSOD mice. We detected enriched levels of Gram-positive bacteria and corresponding decreases in Gram-negative bacteria in 24-wk-old metabolic syndrome-affected TSOD mice compared with age-matched controls...
November 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29158025/oligomycins-a-and-e-major-bioactive-secondary-metabolites-produced-by-streptomyces-sp-strain-hg29-isolated-from-a-saharan-soil
#20
N Khebizi, H Boudjella, C Bijani, N Bouras, H-P Klenk, F Pont, F Mathieu, N Sabaou
An actinobacterial strain, HG29, with potent activity against pathogenic, toxigenic and phytopathogenic fungi was isolated from a Saharan soil sample of Algeria. On the basis of morphological and chemotaxonomic characteristics, the strain was classified in the genus Streptomyces. Analysis of the 16S rRNA gene sequence showed a similarity level of 99.3% with Streptomyces gancidicus NBRC 15412(T). The comparison of its cultural and physiological characteristics with this species revealed significant differences...
November 17, 2017: Journal de Mycologie Médicale
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