keyword
MENU ▼
Read by QxMD icon Read
search

Ferment

keyword
https://www.readbyqxmd.com/read/29333587/genetic-characterization-and-modification-of-a-bioethanol-producing-yeast-strain
#1
Ke Zhang, Ya-Nan Di, Lei Qi, Yang Sui, Ting-Yu Wang, Li Fan, Zhen-Mei Lv, Xue-Chang Wu, Pin-Mei Wang, Dao-Qiong Zheng
Yeast Saccharomyces cerevisiae strains isolated from different sources generally show extensive genetic and phenotypic diversity. Understanding how genomic variations influence phenotypes is important for developing strategies with improved economic traits. The diploid S. cerevisiae strain NY1308 is used for cellulosic bioethanol production. Whole genome sequencing identified an extensive amount of single nucleotide variations and small insertions/deletions in the genome of NY1308 compared with the S288c genome...
January 15, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29333393/effect-of-monascus-fermented-soybean-extracts-on-antioxidant-and-skin-aging-related-enzymes-inhibitory-activities
#2
Yoo-Jeong Jin, Young-Hee Pyo
We investigated the in vitro inhibitory activity against skin aging-related enzymes and antioxidant activity of Monascus-fermented soybean extracts (MFSEs) obtained by using different solvents. The highest Trolox equivalent (TE) antioxidant capacity (3.13±0.06 mM TE/g) and oxygen radical absorbance capacity (2.79±0.09 mM TE/g) of MFSEs were evaluated for the methanol and 80% ethanol extracts, respectively. The antioxidant capacities increased with increasing concentration (0.5~50 mg/mL). In addition, the methanol and 80% ethanol extracts showed an effective inhibition against tyrosinase, hyaluronidase, and elastase compared with those of acetone and hot water extracts (P<0...
December 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/29332141/cultivable-fungi-present-in-antarctic-soils-taxonomy-phylogeny-diversity-and-bioprospecting-of-antiparasitic-and-herbicidal-metabolites
#3
Eldon C Q Gomes, Valéria M Godinho, Débora A S Silva, Maria T R de Paula, Gislaine A Vitoreli, Carlos L Zani, Tânia M A Alves, Policarpo A S Junior, Silvane M F Murta, Emerson C Barbosa, Jaquelline G Oliveira, Fabio S Oliveira, Camila R Carvalho, Mariana C Ferreira, Carlos A Rosa, Luiz H Rosa
Molecular biology techniques were used to identify 218 fungi from soil samples collected from four islands of Antarctica. These consisted of 22 taxa of 15 different genera belonging to the Zygomycota, Ascomycota, and Basidiomycota. Mortierella, Antarctomyces, Pseudogymnoascus, and Penicillium were the most frequently isolated genera and Penicillium tardochrysogenum, Penicillium verrucosus, Goffeauzyma gilvescens, and Mortierella sp. 2 the most abundant taxa. All fungal isolates were cultivated using solid-state fermentation to obtain their crude extracts...
January 13, 2018: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29331852/no-time-to-waste-organic-waste-nanosizing-converts-remains-of-food-processing-into-refined-materials
#4
Sharoon Griffin, Muhammad Sarfraz, Verda Farida, Muhammad Jawad Nasim, Azubuike P Ebokaiwe, Cornelia M Keck, Claus Jacob
Modern food processing results in considerable amounts of side-products, such as grape seeds, walnut shells, spent coffee grounds, and harvested tomato plants. These materials are still rich in valuable and biologically active substances and therefore of interest from the perspective of waste management and "up-cycling". In contrast to traditional, often time consuming and low-value uses, such as vermicomposting and anaerobic digestion, the complete conversion into nanosuspensions unlocks considerable potentials of and new applications for such already spent organic materials without the need of extraction and without producing any additional waste...
January 11, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29331529/effect-of-acids-produced-from-carbohydrate-metabolism-in-cryoprotectants-on-the-viability-of-freeze-dried-lactobacillus-and-prediction-of-optimal-initial-cell-concentration
#5
Shumao Cui, Feng Hang, Xiaoming Liu, Zhiyuan Xu, Zhenmin Liu, Jianxin Zhao, Hao Zhang, Wei Chen
For the industrial production of probiotics powder, various sugars have been used as cryoprotectants to preserve probiotics during freeze-drying. Some of these sugars can be metabolized by Lactobacillus with the production of acids during the mix. In this study, we investigated the effect of acids on ATPase, β-galactosidase, lactate dehydrogenase (LDH), integrity and fluidity of cell membrane and the survival rate of Lactobacillus during freeze-drying. In the presence of Lactobacillus, acids were produced from cryoprotectants containing fermentable sugars before freezing, resulting in a decrease in the pH of the bacterial suspension to below 5...
January 11, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29331528/expression-of-a-thermotolerant-laccase-from-pycnoporus-sanguineus-in-trichoderma-reesei-and-its-application-in-the-degradation-of-bisphenol-a
#6
Jie Zhao, Shenquan Zeng, Ying Xia, Liming Xia
The laccase gene from Pycnoporus sanguineus was cloned and inserted between the strong Pcbh1 promoter and the Tcbh1 terminator from Trichoderma reesei to form the recombinant plasmid pCH-lac. Using Agrobacterium-mediated technique, the pCH-lac was integrated into the chromosomes of T. reesei. Twenty positive transformants were obtained by employing hygromycin B as a selective agent. PCR was used to confirm that the laccase gene was integrated into the chromosomal DNA of T. reesei. Laccase production by recombinant transformants was performed in shaking flasks, and the activity of laccase reached 8...
January 10, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29331526/valerate-production-by-megasphaera-elsdenii-isolated-from-pig-feces
#7
Shota Yoshikawa, Ryosuke Araoka, Yu Kajihara, Toshiyuki Ito, Hirokuni Miyamoto, Hiroaki Kodama
Megasphaera elsdenii is able to produce several short-chain fatty acids (SCFAs), such as acetate, propionate, butyrate, and valerate. These SCFAs serve as an energy source for host animals and play an important role in gut health. In this study, M. elsdenii was isolated from pig feces that had been collected from two farms located in distinct areas of Japan. These M. elsdenii isolates were genotyped, and 7 representative strains were selected. When these 7 strains and M. elsdenii JCM 1772T were cultured with lactate for 24 h, all 7 strains produced valerate as a predominant SCFA...
January 11, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29331525/production-and-characterization-of-ectoine-using-a-moderately-halophilic-strain-halomonas-salina-bcrc17875
#8
Wei-Chuan Chen, Ching-Cha Hsu, John Chi-Wei Lan, Yu-Kaung Chang, Li-Fen Wang, Yu-Hong Wei
This study attempted to utilize Halomonas salina BCRC17875 to produce ectoine by optimizing the agitation speed and medium composition. In addition, the chemical structure of ectoine produced by H. salina BCRC17875 was determined. The results indicate that ectoine production reached 3.65 g/L at 38 h of cultivation when the agitation rate and NaCl concentration were fixed at 200 rpm and 2.0 M, respectively. It reached 9.20 g/L at 44 h of cultivation when the major medium components were yeast extract (56 g/L), glutamate (74...
January 11, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29331518/microwave-assisted-ionic-liquid-mediated-rapid-catalytic-conversion-of-non-edible-lignocellulosic-sunn-hemp-fibres-to-biofuels
#9
Souvik Kumar Paul, Saikat Chakraborty
Sunn hemp fibre - a cellulose-rich crystalline non-food energy crop, containing 75.6% cellulose, 10.05% hemicellulose, 10.32% lignin, with high crystallinity (80.17%) and degree of polymerization (650) - is identified as a new non-food substrate for lignocellulosic biofuel production. Microwave irradiation is employed to rapidly rupture the cellulose's glycosidic bonds and enhance glucose yield to 78.7% at 160 °C in only 46 min. The reactants - long-chain cellulose, ionic liquid, transition metal catalyst, and water - form a polar supramolecular complex that rotates under the microwave's alternating polarity and rapidly dissipates the electromagnetic energy through molecular collisions, thus accelerating glycosidic bond breakage...
January 3, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29331517/tofu-whey-wastewater-is-a-promising-basal-medium-for-microalgae-culture
#10
Shi-Kai Wang, Xu Wang, Jing Miao, Yong-Ting Tian
Tofu whey wastewater (TWW) is an abundant, nutrient riched and safety wastewater and is regarded as an excellent alternative medium in fermentation. In this study, the feasibility of algal cultivation using TWW as the basal medium was investigated. Results indicated that through simple pH adjustment, TWW presented a better culture performance at autotrophic, heterotrophic, and mixotrophic modes compared with that of regular green algae medium, BG-11. The biomass productivities of Chlorella pyrenoidosa at each trophic mode were 4...
January 4, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29331516/simultaneous-saccharification-and-aerobic-fermentation-of-high-titer-cellulosic-citric-acid-by-filamentous-fungus-aspergillus-niger
#11
Weiliang Hou, Jie Bao
Simultaneous saccharification and fermentation (SSF) is the most efficient operation in biorefining conversion, but aerobic SSF under high solids loading significantly faces the serious oxygen transfer limitation. This study took the first insight into an aerobic SSF by high oxygen demanding filamentous fungi in highly viscous lignocellulose hydrolysate. The results show that oxygen requirement in the aerobic SSF by Aspergillus niger was well satisfied for production of cellulosic citric acid. The record high citric acid titer of 136...
January 4, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29331466/high-grain-diets-supplemented-with-phytogenic-compounds-or-autolyzed-yeast-modulate-ruminal-bacterial-community-and-fermentation-in-dry-cows
#12
V Neubauer, R Petri, E Humer, I Kröger, E Mann, N Reisinger, M Wagner, Q Zebeli
The feeding of concentrate-rich diets may lead to microbial imbalances and dysfermentation in the rumen. The main objective of this study was to determine the effects of supplementing phytogenic compounds (PHY) or autolyzed yeast (AY) on rumen fermentation and microbial abundance in cows intermittently fed concentrate-rich diets. The experiment was carried out as an incomplete 3 × 4 Latin square design, with 8 nonlactating rumen-fistulated Holstein-Friesian cows. The cows were randomly assigned to a concentrate diet that was either not supplemented (CON), or supplemented with PHY or AY...
January 10, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29331460/inhibition-of-shigella-sonnei-induced-epithelial-barrier-disruption-by-surface-layer-associated-proteins-of-lactobacilli-from-chinese-fermented-food
#13
Yingchun Zhang, Xiaolu Shi, Siqi Hao, Qianhui Lu, Lanwei Zhang, Xue Han, Weihong Lu
Surface-layer associated proteins (SLAP) of Lactobacillus paracasei ssp. paracasei M5-L and Lactobacillus casei Q8-L were examined to identify the functional basis for their protection within intestinal epithelial cells. The results showed that SLAP of M5-L and Q8-L remained active in a trypsin solution and retained a 45-kDa protein band, similar to that observed in controls. In contrast, under conditions of simulated gastric juice, the SLAP were partially degraded. Inhibitory effects of SLAP on adherence of Shigella sonnei to HT-29 cells were assessed with use of exclusion, competition, and replacement assays...
January 10, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29330912/high-capacity-protein-a-affinity-chromatography-for-the-fast-quantification-of-antibodies-two-wavelength-detection-expands-linear-range
#14
Peter Satzer, Alois Jungbauer
The high-throughput analysis of antibodies from processes can be enhanced when the linear range is expanded and sample preparation is kept to a minimum. We developed a fast chromatography method based on a hexameric variant of Staphylococcal protein A immobilized on Toyopearl matrix, TSK 5 PW using two wavelengths. A protocol with 5 min runtime and a single-wavelength detection at 280 nm yielded an upper limit of quantification of 2.10 mg/mL and a lower limit of quantification of 0.06 mg/mL. The optimized method with a runtime of 2 min and two-wavelength detection at 280 and 300 nm allowed us to span a valid concentration range of 0...
January 13, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29330191/thermophilic-alkaline-fermentation-followed-by-mesophilic-anaerobic-digestion-for-efficient-hydrogen-and-methane-production-from-waste-activated-sludge-focusing-on-dynamics-of-bacterial-pathogens-as-revealed-by-the-combination-of-metagenomic-and-qpcr-analysis
#15
Jingjing Wan, Yuhang Jing, Yue Rao, Shicheng Zhang, Gang Luo
Thermophilic alkaline fermentation followed by mesophilic anaerobic digestion (TM) for hydrogen and methane production from waste activated sludge (WAS) was investigated. TM process was also compared to mesophilic alkaline fermentation followed by mesophilic anaerobic digestion (MM) process and one-stage mesophilic anaerobic digestion (M) process. The results showed that both hydrogen yield (74.5 mLH2/gVS) and methane yield (150.7 mLCH4/gVS) in TM process were higher than those (6.7 mLH2/gVS and 127.8 mLCH4/gVS) in MM process...
January 12, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29330068/engineering-of-hydroxymandelate-synthases-and-the-aromatic-amino-pathway-enables-de-novo-biosynthesis-of-mandelic-and-4-hydroxymandelic-acid-with-saccharomyces-cerevisiae
#16
Mara Reifenrath, Eckhard Boles
Mandelic acid (MA) and 4-hydroxymandelic acid (HMA) are valuable specialty chemicals used as precursors for flavors as well as for cosmetic and pharmaceutical purposes. Today they are mainly synthesized chemically. Their synthesis through microbial fermentation would allow for environmentally sustainable production. In this work, we engineered the yeast Saccharomyces cerevisiae for high-level production of MA and HMA. Expressing the hydroxymandelate synthase from Amycolatopsis orientalis in a yeast wild type strain resulted in the production of 119mg/L HMA from glucose...
January 9, 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/29329945/model-of-acetic-acid-affected-growth-and-poly-3-hydroxybutyrate-production-by-cupriavidus-necator-dsm-545
#17
Jaruwan Marudkla, Wen-Chien Lee, Siwaporn Wannawilai, Yusuf Chisti, Sarote Sirisansaneeyakul
Acetic acid, a potential growth inhibitor, commonly occurs in lignocellulosic hydrolysates. The growth of Cupriavidus necator DSM 545 and production of poly(3-hydroxybutyrate) (PHB) by this bacterium in a glucose-based medium supplemented with various initial concentrations of acetic acid are reported. The bacterium could use both glucose and acetic acid to grow and produce PHB, but acetic acid inhibited growth once its initial concentration exceeded 0.5 g/L. As acetic acid is an unavoidable contaminant in hydrolysates used as sugar sources in commercial fermentations, a mathematical model was developed to describe its impact on growth and the production of PHB...
January 9, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29329863/preparation-of-gentiooligosaccharides-using-trichoderma-viride-%C3%AE-glucosidase
#18
Fei Wang, Jing Wu, Sheng Chen
The recombinant plasmid pPIC9K-bgl1 containing β-glucosidase bgl1 from Trichoderma viride was constructed by overlapping PCR and integrated into Pichia pastoris KM71. In order to assist the formation of disulfide bonds and thus improve protein folding efficiency, protein disulfide isomerase pdi was co-expressed in the P. pastoris KM71/pPIC9K-bgl1/pPICZ-A-pdi strain, and fermentation in flasks resulted in enzyme activity of 143 U/ml. The enzyme activity of β-glucosidase reached 1402 U/ml following optimisation of fermentation conditions in a 3...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29329860/ethyl-carbamate-an-emerging-food-and-environmental-toxicant
#19
REVIEW
Vemana Gowd, Hongming Su, Petr Karlovsky, Wei Chen
Ethyl carbamate (EC), a chemical substance widely present in fermented food products and alcoholic beverages, has been classified as a Group 2A carcinogen by the International Agency for Research on Cancer (IARC). New evidence indicates that long-term exposure to EC may cause neurological disorders. Formation of EC in food and its metabolism have therefore been studied extensively and analytical methods for EC in various food matrices have been established. Due to the potential threat of EC to human health, mitigation strategies for EC in food products by physical, chemical, enzymatic, and genetic engineering methods have been developed...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29329858/mechanical-wheat-flour-modification-and-its-effect-on-protein-network-structure-and-dough-rheology
#20
Stefanie Hackenberg, Mario Jekle, Thomas Becker
Mechanical flour modification is frequently associated with a reduced bread volume due to changed structural and functional properties of protein and starch. To clarify the effect of mechanical flour treatment on the protein network formation at the optimum kneading time (Peaktime), dough was produced with various mechanical starch modification (MSM) levels and visualized by confocal laser scanning microscope before being characterized by protein network analysis (PNA). Dough produced with high MSM showed a reduced branching rate (-14%), a high end-point rate (+25%) and an increased lacunarity (+139%), indicating a poor network connectivity with network interruptions...
May 15, 2018: Food Chemistry
keyword
keyword
34409
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"