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prp preparation

Yuya Nakatani, Hideki Agata, Yoshinori Sumita, Takamitsu Koga, Izumi Asahina
OBJECTIVE: Platelet-rich plasma (PRP) is typically isolated and applied immediately after preparation, making it both a time- and labor-intensive addition to the operative procedure. Thus, it would be convenient if PRP could be preserved. We evaluated the efficacy of freeze-dried PRP (FD-PRP), as compared with freshly isolated PRP (f-PRP) for bone engineering. DESIGN: FD-PRP was prepared by lyophilization of f-PRP and was subsequently preserved at -20°C for one month...
October 15, 2016: Archives of Oral Biology
Hideo Masuki, Toshimitsu Okudera, Taisuke Watanebe, Masashi Suzuki, Kazuhiko Nishiyama, Hajime Okudera, Koh Nakata, Kohya Uematsu, Chen-Yao Su, Tomoyuki Kawase
BACKGROUND: The development of platelet-rich fibrin (PRF) drastically simplified the preparation procedure of platelet-concentrated biomaterials, such as platelet-rich plasma (PRP), and facilitated their clinical application. PRF's clinical effectiveness has often been demonstrated in pre-clinical and clinical studies; however, it is still controversial whether growth factors are significantly concentrated in PRF preparations to facilitate wound healing and tissue regeneration. To address this matter, we performed a comparative study of growth factor contents in PRP and its derivatives, such as advanced PRF (A-PRF) and concentrated growth factors (CGF)...
December 2016: Int J Implant Dent
Mito Kobayashi, Tomoyuki Kawase, Kazuhiro Okuda, Larry F Wolff, Hiromasa Yoshie
BACKGROUND: Platelet-rich fibrin (PRF), a platelet-rich plasma (PRP) derivative mainly composed of fibrin networks, has been increasingly demonstrated to be effective in wound healing in clinical and pre-clinical animal studies. However, there has still been a concern that major growth factors may significantly be loss from PRF during its preparation through the slow clotting process. To address this concern, we compared the angiogenic potential of PRF and PRP by standardization of procedures based on volume ratios...
December 2015: Int J Implant Dent
Matthias Schmitz, Maria Cramm, Franc Llorens, Dominik Müller-Cramm, Steven Collins, Ryuichiro Atarashi, Katsuya Satoh, Christina D Orrù, Bradley R Groveman, Saima Zafar, Walter J Schulz-Schaeffer, Byron Caughey, Inga Zerr
The development and adaption of in vitro misfolded protein amplification systems has been a major innovation in the detection of abnormally folded prion protein scrapie (PrP(Sc)) in human brain and cerebrospinal fluid (CSF) samples. Herein, we describe a fast and efficient protein amplification technique, real-time quaking-induced conversion (RT-QuIC), for the detection of a PrP(Sc) seed in human brain and CSF. In contrast to other in vitro misfolded protein amplification assays-such as protein misfolding cyclic amplification (PMCA)-which are based on sonication, the RT-QuIC technique is based on prion seed-induced misfolding and aggregation of recombinant prion protein substrate, accelerated by alternating cycles of shaking and rest in fluorescence plate readers...
November 2016: Nature Protocols
Carlos Acosta-Olivo, Jorge Elizondo-Rodriguez, Ricardo Lopez-Cavazos, Felix Vilchez-Cavazos, Mario Simental-Mendia, Oscar Mendoza-Lemus
BACKGROUND: Many treatment options for plantar fasciitis currently exist, some with great success in pain relief. The objective of our study was to compare the use of intralesional steroids with platelet-rich plasma (PRP), using pain scales and functional evaluation, in patients with plantar fasciitis who did not respond to conservative treatment. METHODS: A controlled, randomized, blinded clinical assay was performed. Patients were assigned to one of the two groups by selecting a sealed envelope...
October 11, 2016: Journal of the American Podiatric Medical Association
Stefanie Hackl, Alanca Schmid, Christian F W Becker
The site-selective installation of lipid modifications on proteins is critically important in our understanding of how membrane association influences the biophysical properties of proteins as well as to study certain proteins in their native environment. Here, we describe the use of split inteins for the C-terminal attachment of lipid-modified peptides to virtually any protein of interest (POI) via protein trans-splicing (PTS). To achieve this, the protein of interest is expressed in fusion with the N-terminal split intein segment and the C-terminal split intein segment is prepared by solid phase peptide synthesis...
2017: Methods in Molecular Biology
Mubin Hosnuter, Cem Aslan, Daghan Isik, Gorkem Caliskan, Banu Arslan, Mustafa Durgun
BACKGROUND: Platelet-rich plasma (PRP) is increasingly being used in the treatment of chronic wounds, pathologies of the musculoskeletal system, and in cosmetic medicine; however, the preparation of platelet-rich plasma is both time-consuming and requires invasive intervention. Additional costs are introduced if special equipment is used during preparation. The aim of the present study is to test whether autologous platelet-rich plasma (PRP) preserves the feature of growth factor release when stored at -20 °C after preparation...
October 7, 2016: Journal of Plastic Surgery and Hand Surgery
M İrfan Dönmez, Kubilay İnci, Naciye Dilara Zeybek, H Serkan Doğan, Ali Ergen
PURPOSE: To evaluate the early histological effects of the intravesical instillation of platelet-rich plasma (PRP) in rabbit models of interstitial and hemorrhagic cystitis. METHODS: Thirty-six rabbits were classified into 6 groups: saline (S), S+PRP, hydrochloric acid (HCl), HCl+PRP, cyclophosphamide (CyP), and CyP+PRP. At 48 hours after induction, PRP was prepared and intravesically administered to the S+PRP, HCl+PRP, and CyP+PRP groups. Bladder sections were stained with toluidine blue for mast cell counting and with hematoxylin and eosin for histopathology and mitotic index determination...
September 2016: International Neurourology Journal
Geneviève Marcoux, Anne-Claire Duchez, Matthieu Rousseau, Tania Lévesque, Luc H Boudreau, Louis Thibault, Eric Boilard
On activation, platelets release vesicles called microparticles (MPs). MPs are heterogeneous with regard to the presence or absence of mitochondria. We quantified MPs in platelet concentrates (PCs) taking their mitochondrial content into account. Platelet-rich plasma (PRP), buffy coat (BC) and apheresis (AP) PCs were tested through 7 days of storage. A combination of flow cytometry and spanning-tree progression analysis of density-normalized events (SPADE) was used to determine MP and mitochondrial release during storage...
September 29, 2016: Platelets
F Kaya, K Kismet, H Ozer, V G Soylu, M E Duymus, Y A Akgun, A Demir, P Celepli, T Guclu, S Bolat, E Ertas
OBJECTIVES: In this study, it was aimed to investigate whether or not platelet‑rich plasma (PRP) causes intra-abdominal adhesions and therefore, whether or not PRP can be used safely in intra-abdominal operations. METHODS: Of the total of 35 animals, 5 were used as donors for the preparation of platelet‑rich plasma (PRP). The surgical procedures were performed on the remaining 30 animals. These rats were randomized and divided into 3 groups of 10. In Group 1, no adhesion induction was performed...
2016: Bratislavské Lekárske Listy
Nil Guler, Schuharazad Abro, Marty Emanuele, Omer Iqbal, Debra Hoppensteadt, Jawed Fareed
BACKGROUND: Poloxamer-188 (MST-188) is effective in the repair/recovery of damaged cell membranes. MST-188 is a promising agent for protecting blood cell viability. The aim of the study is to test the hypothesis that MST-188 can extend the duration of platelet function. MATERIALS AND METHODS: Blood samples were collected from 20 healthy volunteers. MST-188 (10 or 2 mg/mL) containing platelet-rich plasma (PRP) was prepared with 2 procedures. First, PRP prepared from MST-188 added whole blood (WB); second, MST-188 was added to PRP...
September 20, 2016: Clinical and Applied Thrombosis/hemostasis
Krystin Zeller Meidell, Ryan Robinson, Adriana Vieira-de-Abreu, Adam J Gormley, Hamidreza Ghandehari, David W Grainger, Robert A Campbell
Integrin-targeting peptide RGDfK-labeled gold nanorods (GNR) seek to improve hyperthermia targeted to solid tumors by exploiting the known up-regulation of integrin αvβ3 cell membrane proteins on solid tumor vasculature surfaces. Tumor binding specificity might be expected since surrounding tissues and endothelial cells have limited numbers of these receptors. However, RGD peptide binding to many proteins is promiscuous, with known affinity to several families of cell integrin receptors, and also possible binding to platelets after intravenous infusion via a different integrin receptor, αIIbβ3, on platelets...
September 20, 2016: Journal of Biomedical Materials Research. Part A
Lotta Granqvist, Pasi Virta
2'-O-[(4-Trifluoromethyl-triazol-1-yl)methyl] reporter groups have been incorporated into guanosine-rich RNA models (including a known bistable Qd/Hp RNA and two G-rich regions of mRNA of human prion protein, PrP) and applied for the (19) F NMR spectroscopic characterization of plausible G-quadruplex/hairpin (Qd/Hp) transitions in these RNA structures. For the synthesis of the CF3 -labeled RNAs, phosphoramidite building blocks of 2'-O-[(4-CF3 -triazol-1-yl)methyl] nucleosides (cytidine, adenosine, and guanosine) were prepared and used as an integral part of the standard solid-phase RNA synthesis...
October 17, 2016: Chemistry: a European Journal
Ahmet Akgül, Musa Cirak, Tansu Birinci
Platelet-rich plasma (PRP) is an autologous concentrated preparation of human platelets contained in a small volume of plasma that is characterized by hemostatic and tissue-repairing effects. Being enriched by various kinds of growth factors, and their tissue-repairing effects have made them the focus of attention for use in tissue regeneration. PRP has been safely used and documented in many different fields, including orthopedics, sports injuries, dental and periodontal surgery, and cosmetic, plastic, cardiovascular, general, and maxillofacial surgery...
September 6, 2016: Lymphatic Research and Biology
Betul Gozel Ulusal
BACKGROUND: Cosmetic applications of platelet-rich plasma (PRP) are new, and reports are scarce and dispersed in the literature. There are a variety of commercially available kits and injection techniques, and the number and intervals of injections vary. New investigations should focus on developing a standardized procedure for PRP preparation and application methods to augment its efficacy and potency. OBJECTIVES: In this report, we aim to provide data and commentary to assist and add to current guidelines...
September 5, 2016: Journal of Cosmetic Dermatology
Aneta Wrzyszcz, Joanna Urbaniak, Agnieszka Sapa, Mieczysław Woźniak
To date, there has been no ideal method for blood platelet isolation which allows one to obtain a preparation devoid of contaminations, reflecting the activation status and morphological features of circulating platelets. To address these requirements, we have developed a method which combines the continuous density gradient centrifugation with washing from PGI2-supplemented platelet-rich plasma (PRP). We have assessed the degree of erythrocyte and leukocyte contamination, recovery of platelets, morphological features, activation status, and reactivity of isolated platelets...
September 2, 2016: Platelets
Oscar Rubini Ávila, Natália Gomes Parizzi, Ana Paula Mayumi Souza, Dayane Silvestre Botini, João Ytimura Alves, Silvio Henrique Maia Almeida
INTRODUCTION: The platelet-rich plasma (PRP) is part of a set of biotechnologies, providing some growth factors that promote repair of different tissues. The polypropylene meshes (PPM) are applied in the correction of abdominal defects, pelvic floor and urinary incontinence, however, they induce many significant complications, as a result of an inappropriate inflammatory response. PURPOSE: To investigate the changes caused by PRP associated with the implantation of PPM in the abdomen of female rabbits, in the production of collagen I and III and the inflammatory infiltrate (ININ)...
September 1, 2016: International Braz J Urol: Official Journal of the Brazilian Society of Urology
Andrew L Frelinger, Anja J Gerrits, Allen L Garner, Andrew S Torres, Antonio Caiafa, Christine A Morton, Michelle A Berny-Lang, Sabrina L Carmichael, V Bogdan Neculaes, Alan D Michelson
BACKGROUND: Activated autologous platelet-rich plasma (PRP) used in therapeutic wound healing applications is poorly characterized and standardized. Using pulsed electric fields (PEF) to activate platelets may reduce variability and eliminate complications associated with the use of bovine thrombin. We previously reported that exposing PRP to sub-microsecond duration, high electric field (SMHEF) pulses generates a greater number of platelet-derived microparticles, increased expression of prothrombotic platelet surfaces, and differential release of growth factors compared to thrombin...
2016: PloS One
Seyed Fazlolah Mousavi, Sara Fatemi, Seyed Davar Siadat, Seyed Mohsen Zahraei, Elnaz Nikanpour, Mohamad Ali Malekan, Ali Reza Khabiri, Ali Reza Janani
BACKGROUND: Haemophilus influenzae type b (Hib) infection has high morbidity and mortality rate, especially in children under 5 years of age. Enzyme-linked immunosorbent assay (ELISA) technique is the most used method to detect antibodies against H. influenzae. Available commercial ELISA kits are expensive and not always readily available, particularly for epidemiological studies. OBJECTIVES: This study was performed to develop and optimize a homemade ELISA kit for the detection of Hib anti-polyribosylribitol phosphate (PRP) antibodies in children...
May 2016: Jundishapur Journal of Microbiology
Marwa Ahmed, Sherif A Reffat, Amany Hassan, Fikry Eskander
BACKGROUND: Diabetic foot ulcer is considered as a major health problem that predisposes to limb amputation. Among the different methods to achieve ulcer healing, platelet-rich plasma (PRP) gel is gaining popularity. It is thought to stimulate wound closure by providing essential growth factors for healing. This study aims to evaluate the value of autologous PRP gel in the treatment of diabetic ulcers. METHODS: The study included 56 patients of both sex from 18 to 80 years, with clean chronic diabetic foot ulcers divided into 2 equal groups...
August 12, 2016: Annals of Vascular Surgery
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