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https://www.readbyqxmd.com/read/29790267/systematic-review-of-platelet-rich-plasma-prp-preparation-and-composition-for-the-treatment-of-androgenetic-alopecia
#1
REVIEW
MaryJo E Kramer, Terrence C Keaney
BACKGROUND: Platelet-rich plasma (PRP) injections have gained popularity in dermatology practice as a treatment for hair loss. As an autologous blood product, PRP is categorized as a minimally manipulated tissue by the U.S. Food and Drug Administration (FDA) and thus evades extensive regulation. As a result, there is a lack of standardization of its preparation and final composition. OBJECTIVE: This study aims to provide a systematic review of the various PRP preparation protocols and PRP compositions utilized in clinical trials for the treatment of hair loss...
May 22, 2018: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/29785390/platelet-rich-plasma-prp-for-the-treatment-of-vulvar-lichen-sclerosus-in-a-premenopausal-woman-a-case-report
#2
D Franic, Z Iternička, M Franić-Ivanišević
The use of platelet-rich plasma (PRP) for the treatment of lichen sclerosus (LS) in a 38-year-old premenopausal woman is reported. The diagnosis was confirmed histologically and the symptoms documented using the ICIQ Vaginal Symptoms Questionnaire (ICIQ-VS) and the Female Sexual Function Index (FSFI) questionnaire. PRP was prepared from autologous blood using the Regen Cellular Matrix Kit. PRP was administered twice over two months. Histology at follow-up one month after the second administration showed the epidermis was nearly normal and upper dermal cellularity had been restored...
April 2018: Case Reports in Women's Health
https://www.readbyqxmd.com/read/29766749/enhanced-sternal-healing-via-platelet-rich-plasma-and-biodegradable-gelatin-hydrogel
#3
Masafumi Shibata, Gen Takagi, Mitsuhiro Kudo, Jiro Kurita, Yoko Kawamoto, Yasuo Miyagi, Mikimoto Kanazashi, Takashi Sakatani, Zenya Naito, Yasuhiko Tabata, Masaaki Miyamoto, Takashi Nitta
Platelet-rich plasma (PRP) contains numerous growth factors and promotes bone fracture healing. The aim of this study was to evaluate the effectiveness of the controlled release of PRP from biodegradable gelatin hydrogel for promoting healing in a rabbit ischemic sternal model. PRP was prepared from the whole blood of a Japanese white rabbit. Sixteen rabbits were randomized into four groups (each n = 4) and all underwent median sternotomy and bilateral internal thoracic artery removal. Before the sternum was closed, the following solutions were applied between the sternum incisions in three of the groups: 30 mg of gelatin hydrogel incorporating 300 μL of phosphate-buffered saline, 300 μL of a solution form of PRP, or 30 mg of gelatin hydrogel incorporating 300 μL of PRP (PRP+Gel)...
May 16, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29760725/the-effects-of-hyperacute-serum-on-the-elements-of-the-human-subchondral-bone-marrow-niche
#4
Melinda Simon, Bálint Major, Gabriella Vácz, Olga Kuten, István Hornyák, Adél Hinsenkamp, Dorottya Kardos, Marcell Bagó, Domonkos Cseh, Adrienn Sárközi, Denes Horvathy, Stefan Nehrer, Zsombor Lacza
Mesenchymal stem cells (MSCs) are widely used in laboratory experiments as well as in human cell therapy. Their culture requires animal sera like fetal calf serum (FCS) as essential supplementation; however, animal sera pose a risk for clinical applications. Human blood derivatives, for example, platelet-rich plasma (PRP) releasates, are potential replacements of FCS; however, it is unclear which serum variant has the best effect on the given cell or tissue type. Additionally, blood derivatives are commonly used in musculoskeletal diseases like osteoarthritis (OA) or osteonecrosis as "proliferative agents" for the topical MSC pool...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29741923/effect-of-intraoperative-platelet-rich-plasma-treatment-on-postoperative-donor-site-knee-pain-in-patellar-tendon-autograft-anterior-cruciate-ligament-reconstruction-a-double-blind-randomized-controlled-trial
#5
Brian L Walters, David A Porter, Sarah J Hobart, Benjamin B Bedford, Daniel E Hogan, Malachy M McHugh, Devon A Klein, Kendall Harousseau, Stephen J Nicholas
BACKGROUND: Donor site morbidity in the form of anterior knee pain is a frequent complication after bone-patellar tendon-bone (BPTB) autograft anterior cruciate ligament (ACL) reconstruction. Hypothesis/Purpose: The purpose was to examine the effect of the intraoperative administration of platelet-rich plasma (PRP) on postoperative kneeling pain. It was hypothesized that PRP treatment would reduce knee pain. STUDY DESIGN: Randomized controlled trial; Level of evidence, 2...
May 1, 2018: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29740270/therapeutic-effect-of-platelet-rich-plasma-in-rat-spinal-cord-injuries
#6
Nan-Fu Chen, Chun-Sung Sung, Zhi-Hong Wen, Chun-Hong Chen, Chien-Wei Feng, Han-Chun Hung, San-Nan Yang, Kuan-Hao Tsui, Wu-Fu Chen
Platelet-rich plasma (PRP) is prepared by centrifuging fresh blood in an anticoagulant state, and harvesting the platelet-rich portion or condensing platelets. Studies have consistently demonstrated that PRP concentrates are an abundant source of growth factors, such as platelet-derived growth factor (PDGF), transforming growth factor β (TGF-β), insulin-like growth factor 1 (IGF-1), and epithelial growth factor (EGF). The complex mechanisms underlying spinal cord injury (SCI) diminish intrinsic repair and neuronal regeneration...
2018: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/29736577/prp-therapy
#7
Ibrahim Fatih Cengiz, J Miguel Oliveira, Rui L Reis
Osteochondral lesions remain as a clinical challenge despite the advances in orthopedic regenerative strategies. Biologics, in particular, platelet-rich plasma, has been applied for the reparative and regenerative effect in many tissues, and osteochondral tissue is not an exception. Platelet-rich plasma is an autologous concentrate prepared from the collected blood; thus, this safe application is free of immune response or risk of transmission of disease. It has a high potential to promote regeneration, thanks to its content, and can be applied alone or can reinforce a tissue engineering strategy...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29713784/limited-evidence-for-adipose-derived-stem-cell-therapy-on-the-treatment-of-osteoarthritis
#8
REVIEW
Eoghan T Hurley, Youichi Yasui, Arianna L Gianakos, Dexter Seow, Yoshiharu Shimozono, Gino M M J Kerkhoffs, John G Kennedy
PURPOSE: The purpose of this systematic review is to evaluate the effects of adipose derived mesenchymal stem cells (ADSCs) in the treatment of osteoarthritis (OA) in the clinical setting. METHODS: A literature search was performed in the MEDLINE, EMBASE, and The Cochrane Library Database up to January 2017 for inclusion and exclusion criteria. Criteria for inclusion were clinical studies demonstrating the effects of ADSCs on OA, and written in English. The following variables were analyzed: donor site, volume of adipose tissue, preparation of ADSCs, clinical outcomes, and complication rate...
April 30, 2018: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/29713597/the-role-of-prp-and-adipose-tissue-derived-keratinocytes-on-burn-wound-healing-in-diabetic-rats
#9
Navid Hosseini Mansoub, Mehmet Gürdal, Elif Karadadaş, Hilal Kabadayi, Seda Vatansever, Gulinnaz Ercan
Introduction: Diabetic burn wounds and ulcers are significant complications of diabetic patients. The aim of this study is to investigate the use of platelet rich-plasma (PRP) and/or keratinocyte-like cells (KLCs) in diabetic thermal wound rat model and to evaluate EGF, FGF-2, TGF-β1, COL1α2, MCP-1 and VEGF-α as wound healing markers at gene expression level. Method: In this study, we used adipose tissue as the source of mesenchymal stem cells (MSCs) and differentiated MSCs into KLCs. KLCs were characterized and transferred to the burn areas on the dorsum of streptozotocine (STZ)-induced diabetic rats...
2018: BioImpacts: BI
https://www.readbyqxmd.com/read/29684380/sustained-or-higher-levels-of-growth-factors-in-platelet-rich-plasma-during-7-day-storage
#10
Ying-Hao Wen, Wan-Ying Lin, Chi-Jui Lin, Yu-Chen Sun, Pi-Yueh Chang, Hsin-Yao Wang, Jang-Jih Lu, Wen-Lin Yeh, Tzong-Shi Chiueh
BACKGROUND: The effectiveness of platelet-rich plasma (PRP) for treating soft tissue injuries is still controversial. Most of PRPs were prepared simply by concentrating in volume and were injected right after preparation in physician offices. Neither platelet count nor growth factors were quantitated in advance. We prepared and stored leukocyte and platelet-rich plasma (L-PRP) by regular separation protocols for blood components in the blood bank. And we investigated the dynamic change of growth factors in the L-PRPs over the period of storage...
April 20, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29682351/comparative-analysis-of-cellular-and-growth-factor-composition-in-bone-marrow-aspirate-concentrate-and-platelet-rich-plasma
#11
Hisashi Sugaya, Tomokazu Yoshioka, Toshiki Kato, Yu Taniguchi, Hiroshi Kumagai, Kojiro Hyodo, Osamu Ohneda, Masashi Yamazaki, Hajime Mishima
The purpose of this study was to quantify the stem cell and growth factor (GF) contents in the bone marrow aspirate concentrate (BMAC) and platelet-rich plasma (PRP) prepared from whole blood using a protocol established in our laboratory. We examined 10 patients with osteonecrosis of the femoral head who were treated by autologous BMAC transplantation at our hospital between January 2015 and June 2015. We quantified CD34+ and CD31-CD45-CD90+CD105+ cells in BMAC and PRP by flow cytometry. Additionally, we measured various GFs, that is, basic fibroblast growth factor (b-FGF), platelet-derived growth factor-BB (PDGF-BB), vascular endothelial growth factor (VEGF), transforming growth factor- β 1 (TGF- β 1), and bone morphogenetic protein-2 (BMP-2) in BMAC and PRP using enzyme-linked immunosorbent assays and statistical analyses...
2018: Bone Marrow Research
https://www.readbyqxmd.com/read/29608156/effects-of-allogeneic-platelet-rich-plasma-prp-on-the-healing-process-of-sectioned-achilles-tendons-of-rats-a-methodological-description
#12
Laura Greimers, Pierre V Drion, Alain Colige, Vincent Libertiaux, Vincent Denoël, Christelle Lecut, André Gothot, Jean-François Kaux
This article describes the experimental procedures used to observe if PRP can positively affect tendon healing. There are 4 main steps to follow: induce a lesion in the Achilles tendon; prepare PRP and inject it (or the saline solution); remove the tendon; and perform biomechanical, molecular, and histological evaluations. At each step, all the procedures and methods are described in detail, so they can be reproduced easily. Achilles tendons have been surgically sectioned (removal of a 5-mm long section). Afterwards, PRP or saline solution was injected to study whether PRP has a positive effect on the healing of the tendon...
March 19, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29581613/intra-articular-platelet-rich-plasma-prp-injections-for-treating-knee-pain-associated-with-osteoarthritis-of-the-knee-in-the-japanese-population-a-phase-i-and-iia-clinical-trial
#13
Yu Taniguchi, Tomokazu Yoshioka, Akihiro Kanamori, Katsuya Aoto, Hisashi Sugaya, Masashi Yamazaki
Intra-articular platelet-rich plasma (PRP) injection has been found to be effective for treating osteoarthritis in patients from Western countries; however, the safety and efficacy of PRP have not been sufficiently investigated in Japanese patients. The present study aimed to evaluate the safety and feasibility of intra-articular PRP injection in Japanese patients with knee osteoarthritis. PRP without white blood cells was prepared using a single-spin centrifuge (PRGF-Endoret; BTI Biotechnology Institute, Vitoria, Spain)...
February 2018: Nagoya Journal of Medical Science
https://www.readbyqxmd.com/read/29575062/restored-response-to-adp-downstream-of-purinergic-p2y-12-receptor-in-apheresis-platelets-after-pathogen-reducing-xenon-flash-treatment
#14
Hideki Abe, Takaaki Abe, Masayuki Shiba, Masahiro Satake
BACKGROUND: Our previous study revealed that pathogen-reducing filtered xenon flash-treated platelets (fXe-PLTs) showed sustained aggregation in response to adenosine diphosphate (ADP), but apheresis-collected PLTs (Aph-PLTs) showed reversible aggregation. STUDY DESIGN AND METHODS: Aph-PLTs, fXe-PLTs, and freshly prepared PLTs (PRP-PLTs) from whole blood were used to investigate the following responses to ADP: concentration response and effects of ADP receptor antagonists on aggregation, the cytosolic calcium (Ca2+ ) flux downstream of P2Y1 receptor signaling, and phosphorylation of vasodilator-stimulated phosphoprotein (VASP) and signaling intermediate protein Akt downstream of the P2Y12 receptor...
March 25, 2018: Transfusion
https://www.readbyqxmd.com/read/29574015/effects-of-platelet-rich-plasma-on-tissue-engineered-vascularized-flaps-in-an-in-vivo-chamber
#15
Yoshio Tanaka, Yusuke Hamamoto, Aizezi Niyazi, Tomohisa Nagasao, Masaki Ueno, Yasuhiko Tabata
AIMS: We investigated the reproducibility of creating a vascularized tissue flap in an in vivo tissue engineering chamber by incubating a vascular pedicle imbedded in a collagen sponge with activated platelet-rich plasma (aPRP) and basic fibroblast growth factor (bFGF). METHODS: Collagen sponge soaked with saline (control group), bFGF (Group 1), aPRP (Group 2), and aPRP/controlled release bFGF (Group 3) was implanted with a saphenous arteriovenous pedicle into a tissue engineering chamber, located subcutaneously in the groin of rabbits...
March 7, 2018: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/29511946/adipose-tissue-extract-shows-potential-for-wound-healing-in-vitro-proliferation-and-migration-of-cell-types-contributing-to-wound-healing-in-the-presence-of-adipose-tissue-preparation-and-platelet-rich-plasma
#16
Jenny F López, Jertta-Riina Sarkanen, Outi Huttala, Ilkka S Kaartinen, Hannu O Kuokkanen, Timo Ylikomi
Growth factors are the key elements in wound healing signaling for cell migration, differentiation and proliferation. Platelet-rich plasma (PRP), one of the most studied sources of growth factors, has demonstrated to promote wound healing in vitro and in vivo. Adipose tissue is an alternative source of growth factors. Through a simple lipoaspirate method, adipose derived growth factor-rich preparation (adipose tissue extract; ATE) can be obtained. The authors set out to compare the effects of these two growth factor sources in cell proliferation and migration (scratch) assays of keratinocyte, fibroblast, endothelial and adipose derived stem cells...
March 6, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29508105/decellularized-amnion-scaffold-with-activated-prp-a-new-paradigm-dressing-material-for-burn-wound-healing
#17
Jeevitaa Kshersagar, Ravi Kshirsagar, Shashikant Desai, Raghvendra Bohara, Meghnad Joshi
Direct application of amnion has greater risk of immunological rejection and infection. Decellularization is an effective method to lower the risk of immune complications and infections. The bioreactor assembly with multiple cassettes was designed for decellurization of multiple amnions with different cell types simultaneously in single run. A detergent-based protocol was modified to remove all cellular components from amnion and diminish the DNA content to render it non-immunogenic. Amnion (n = 10) were treated with 2% sodium dodecyl sulphate (SDS), 5% dimethyl sulfoxide (DMSO) and 2% sodium deoxycholeate (SD)...
March 5, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29503690/efficacy-of-platelet-rich-plasma-for-bone-fusion-in-transforaminal-lumbar-interbody-fusion
#18
Go Kubota, Hiroto Kamoda, Sumihisa Orita, Kazuhidee Inage, Michihiro Ito, Masaomi Yamashita, Takeo Furuya, Tsutomu Akazawa, Yasuhiro Shiga, Seiji Ohtori
Study Design: Retrospective case series. Purpose: To examine the efficacy of platelet-rich plasma (PRP) for bone fusion in transforaminal lumbar interbody fusion (TLIF) using local bone grafting. Overview of Literature: Several authors have reported the efficacy of PRP for bone union in animal models. However, the use of PRP for bone fusion in TLIF surgery has not been fully explored. Methods: Twenty patients underwent single-level TLIF surgery because of L4 spondylolisthesis...
February 2018: Asian Spine Journal
https://www.readbyqxmd.com/read/29473280/effect-of-platelets-rich-plasma-on-scalp-hair-diameter
#19
Muhammad Ahmad
OBJECTIVES: To measure the effects of platelet-rich plasma (PRP) on hair diameter in Pakistani population. MATERIALS AND METHODS: The study was conducted in a private setup in the adult patients undergoing PRP treatment. Baseline platelets count was noted for each patient. PRP was prepared using a commercial kit. Three PRP injections were administered in the scalp at 0, 1, and 3 months of interval. The hair caliber was measured in the mid-scalp area using a separate stencil for each patient...
February 23, 2018: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/29464105/osteochondral-defects-of-the-talus-with-a-focus-on-platelet-rich-plasma-as-a-potential-treatment-option-a-review
#20
REVIEW
Ahmed Aly Elghawy, Carlos Sesin, Michael Rosselli
Objective: To provide a review of osteochondral lesions of the talus, to discuss the evidence of the risks and benefits of platelet-rich plasma (PRP) as a viable treatment option, and to measure the efficacy of PRP using MRI evidence of cartilage regeneration, as well as scales that measure improvement in 'pain' and 'functionality'. Eligibility criteria: Studies that use PRP in either conservative or intraoperative settings to treat osteochondral defects of the talus...
2018: BMJ Open Sport & Exercise Medicine
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