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https://www.readbyqxmd.com/read/28910420/role-of-the-central-lysine-cluster-and-scrapie-templating-in-the-transmissibility-of-synthetic-prion-protein-aggregates
#1
Bradley R Groveman, Gregory J Raymond, Katrina J Campbell, Brent Race, Lynne D Raymond, Andrew G Hughson, Christina D Orrú, Allison Kraus, Katie Phillips, Byron Caughey
Mammalian prion structures and replication mechanisms are poorly understood. Most synthetic recombinant prion protein (rPrP) amyloids prepared without cofactors are non-infectious or much less infectious than bona fide tissue-derived PrPSc. This effect has been associated with differences in folding of the aggregates, manifested in part by reduced solvent exclusion and protease-resistance in rPrP amyloids, especially within residues ~90-160. Substitution of 4 lysines within residues 101-110 of rPrP (central lysine cluster) with alanines (K4A) or asparagines (K4N) allows formation of aggregates with extended proteinase K (PK) resistant cores reminiscent of PrPSc, particularly when seeded with PrPSc...
September 14, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28904970/responders-to-platelet-rich-plasma-in-osteoarthritis-a-technical-analysis
#2
REVIEW
Christophe Milants, Olivier Bruyère, Jean-François Kaux
PURPOSE: To evaluate the similarities and differences between the variety of platelet-rich plasma (PRP) formulations, preparation, and uses to try to determine the best responses for the treatment of knee osteoarthritis. MATERIALS AND METHODS: A comparison of the outcomes of randomized controlled trials (RCTs) included in the 3 most recent and high-quality meta-analyses to classify the different studies in 2 groups (bad responders group (BRG) and very good responders group (VGRG))...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28887865/plasma-rich-plasma-the-ultimate-secret-for-youthful-skin-elixir-and-hair-growth-triggering
#3
REVIEW
Ebtisam Elghblawi
The clinical application of platelet-rich plasma (PRP) is based on the increase in the concentration of growth factors that are released from alpha-granule of the concentrated platelets and in the secretion of proteins which are able to capitalize on the healing process at the cellular level. It has been invented to restore the natural beauty by starting the natural rejuvenation process of the skin and aiming to make it function as a younger one and to keep the skin youthful and maintain it. Besides that, it is also emerged to include hairs as a new injectable procedure to enable stimulating hair growth locally and topically; preventing its fall; improving hair shaft, hair stem, and its caliber; increasing its shine, vitality, and pliability; and declining hair splitting and breakage...
September 8, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28861796/preparation-and-immunostaining-of-the-myenteric-plexus-of-prion-infected-mice
#4
Laura J Ellett, Victoria A Lawson
Intracerebral inoculation of mice with the M1000 strain of mouse-adapted human prions results in the consistent accumulation of PrP(Sc) in the ileum of the gastrointestinal tract (GIT) of mice with clinical signs of prion disease. The accumulation of PrP(Sc) in the ileum is accompanied by caspase activation and loss of immunoreactivity in subpopulations of neurons in the enteric nervous system. This suggests that like neurons in the central nervous system, cells in the enteric nervous system are also susceptible to prion-induced toxicity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28861795/strain-typing-of-prion-diseases-using-in-vivo-mouse-models
#5
Aileen Boyle, Kris Hogan, Jean C Manson, Abigail B Diack
Transmissible spongiform encephalopathies (TSE) or prion diseases exhibit strain variation, a phenomenon that has been studied extensively in mouse bioassays. Despite the introduction of many rapid in vitro systems, bioassays remain a key tool in defining prion strains and their ability to transmit disease in vivo. Prion strains can be characterized by a range of phenotypic characteristics such as incubation period, vacuolar pathology, and distribution of the abnormal form of PrP following experimental transmission of the agent into a panel of mice (transgenic or wild type)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28861781/analysis-of-prion-protein-structure-using-nuclear-magnetic-resonance-spectroscopy
#6
Ivana Biljan, Gregor Ilc, Janez Plavec
Nuclear magnetic resonance (NMR) spectroscopy is a powerful experimental tool for obtaining information on three-dimensional (3D) structures of proteins at atomic resolution. In inherited forms of prion diseases, misfolding of cellular prion protein, PrP(C), into its pathological form, PrP(Sc), is caused by mutations in the human prion protein gene (PRNP). Understanding of the earliest stages of the conformational changes leading to spontaneous generation of prions in inherited forms of prion diseases may benefit from detailed structural analysis of different human (Hu) PrP variants...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28861778/purification-and-fibrillation-of-full-length-recombinant-prp
#7
Natallia Makarava, Regina Savtchenko, Ilia V Baskakov
Misfolding and aggregation of prion protein are related to several neurodegenerative diseases in humans such as Creutzfeldt-Jakob disease, fatal familial insomnia, and Gerstmann-Straussler-Scheinker disease. A growing number of applications in the prion field including assays for detection of PrP(Sc) and methods for production of PrP(Sc) de novo require recombinant prion protein (PrP) of high purity and quality. Here, we report an experimental procedure for expression and purification of full-length mammalian prion protein...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28860871/first-in-man-intraglandular-implantation-of-stromal-vascular-fraction-and-adipose-derived-stem-cells-plus-platelet-rich-plasma-in-irradiation-induced-gland-damage-a-case-study
#8
Kristin Comella, Walter Bell
BACKGROUND: Stromal vascular fraction (SVF) is a mixture of cells which can be isolated from a mini-lipoaspirate of fat tissue. Platelet-rich plasma (PRP) is a mixture of growth factors and other nutrients which can be obtained from peripheral blood. Adipose-derived stem/stromal cells (ADSCs) can be isolated from fat tissue and expanded in culture. The SVF includes a variety of different cells such as ADSCs, pericytes, endothelial/progenitor cells, and a mix of different growth factors...
2017: International Medical Case Reports Journal
https://www.readbyqxmd.com/read/28852294/a-spilt-head-study-of-efficacy-of-placebo-versus-platelet-rich-plasma-injections-in-the-treatment-of-androgenic-alopecia
#9
Dilip Kachhawa, Gauri Vats, Durgesh Sonare, Pankaj Rao, Sandeep Khuraiya, Rohit Kataiya
BACKGROUND: Platelet-rich plasma (PRP) is an autologous concentration of human platelets contained in a small volume of plasma with haemostatic and tissue repairing effects. Being enriched by various growth factors, PRP has become the focus of attention in numerous fields of medicine. Androgenic alopecia (AGA) is a common chronic hair loss disorder, characterised by progressive hair loss. Despite the therapeutic options available, there is low patient compliance and satisfaction rate...
April 2017: Journal of Cutaneous and Aesthetic Surgery
https://www.readbyqxmd.com/read/28831830/biological-treatment-of-the-knee-with-platelet-rich-plasma-or-bone-marrow-aspirate-concentrates
#10
Gilbert Moatshe, Elizabeth R Morris, Mark E Cinque, Cecilia Pascual-Garrido, Jorge Chahla, Lars Engebretsen, Robert F Laprade
- Knee pathologies including focal cartilage injuries, osteoarthritis (OA), and ligament injuries are common. The poor regeneration and healing potential of cartilage has led to the search for other treatment modalities with improved healing capacity. Furthermore, with an increasing elderly population that desires to remain active, the burden of knee pathologies is expected to increase. Increased sports participation and the desire to return to activities faster is also demanding more effective and minimally invasive treatment options...
August 23, 2017: Acta Orthopaedica
https://www.readbyqxmd.com/read/28823058/polyurethane-gelatin-nanofibrils-neural-guidance-conduit-containing-platelet-rich-plasma-and-melatonin-for-transplantation-of-schwann-cells
#11
Majid Salehi, Mahdi Naseri-Nosar, Somayeh Ebrahimi-Barough, Mohammdreza Nourani, Arash Khojasteh, Saeed Farzamfar, Korosh Mansouri, Jafar Ai
The current study aimed to enhance the efficacy of peripheral nerve regeneration using a biodegradable porous neural guidance conduit as a carrier to transplant allogeneic Schwann cells (SCs). The conduit was prepared from polyurethane (PU) and gelatin nanofibrils (GNFs) using thermally induced phase separation technique and filled with melatonin (MLT) and platelet-rich plasma (PRP). The prepared conduit had the porosity of 87.17 ± 1.89%, the contact angle of 78.17 ± 5.30° and the ultimate tensile strength and Young's modulus of 5...
August 19, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28805478/production-of-platelet-rich-plasma-gel-from-elderly-patients-under-antithrombotic-drugs-perspectives-in-chronic-wounds-care
#12
M Velier, J Magalon, A Daumas, M Cassar, P Francois, A Ghazouane, C Philandrianos, B Bertrand, C Frere, D Bernot, P Villani, F Dignat George, F Sabatier
Platelet-Rich Plasma (PRP) is an autologous biological therapy obtained by centrifuging the patient's own blood to concentrate platelets. The addition of autologous thrombin and calcium chloride to PRP allows the production of a semi-solid form called PRP gel. PRP gel is increasingly used in a variety of tissue defects and predominantly in the management of non-healing chronic wounds. The topical application of PRP gel seems promising due to the capability of platelets to store and secrete growth factors (GF), fibrin and cytokines, which are essentials for wound healing...
August 14, 2017: Platelets
https://www.readbyqxmd.com/read/28773189/evaluation-of-3d-printed-polycaprolactone-scaffolds-coated-with-freeze-dried-platelet-rich-plasma-for-bone-regeneration
#13
Junda Li, Meilin Chen, Xiaoying Wei, Yishan Hao, Jinming Wang
Three-dimensional printing is one of the most promising techniques for the manufacturing of scaffolds for bone tissue engineering. However, a pure scaffold is limited by its biological properties. Platelet-rich plasma (PRP) has been shown to have the potential to improve the osteogenic effect. In this study, we improved the biological properties of scaffolds by coating 3D-printed polycaprolactone (PCL) scaffolds with freeze-dried and traditionally prepared PRP, and we evaluated these scaffolds through in vitro and in vivo experiments...
July 19, 2017: Materials
https://www.readbyqxmd.com/read/28762630/comparison-of-multiple-electrode-aggregometry-with-lumi-aggregometry-for-the-diagnosis-of-patients-with-mild-bleeding-disorders
#14
R Al Ghaithi, S Drake, S P Watson, N V Morgan, P Harrison
BACKGROUND: Multiple electrode aggregometry (MEA) measures changes in electrical impedance caused by platelet aggregation in whole blood. This approach is faster, more convenient and offers the advantage over light transmission aggregometry (LTA) of assessing platelet function in whole blood and reducing pre-analytical errors associated with preparation of platelet rich plasma (PRP). Several studies indicate the utility of this method in assessing platelet inhibition in individuals taking antiplatelet agents e...
August 1, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28761774/proinflammatory-and-anabolic-gene-expression-effects-of-platelet-rich-gel-supernatants-on-equine-synovial-membrane-explants-challenged-with-lipopolysaccharide
#15
Jorge U Carmona, Diana L Ríos, Catalina López, María E Álvarez, Jorge E Pérez
Platelet-rich plasma (PRP) preparations are used in horses with osteoarthritis (OA). However, some controversies remain regarding the ideal concentration of platelets and leukocytes to produce an adequate anti-inflammatory and anabolic response in the synovial membrane. The aims of this study were to study the influence of leukoconcentrated platelet-rich gel (Lc-PRG) and leukoreduced platelet-rich gel (Lr-PRG) supernatants on the quantitative expression of some proinflammatory and anabolic genes in equine synovial membrane explants (SMEs) challenged with lipopolysaccharide (LPS)...
2017: Veterinary Medicine International
https://www.readbyqxmd.com/read/28735763/platelet-rich-plasma-enhances-bone-union-in-posterolateral-lumbar-fusion-a-prospective-randomized-controlled-trial
#16
Go Kubota, Hiroto Kamoda, Sumihisa Orita, Kazuyo Yamauchi, Yoshihiro Sakuma, Yasuhiro Oikawa, Kazuhide Inage, Takeshi Sainoh, Jun Sato, Michihiro Ito, Masaomi Yamashita, Junichi Nakamura, Takane Suzuki, Kazuhisa Takahashi, Seiji Ohtori
BACKGROUND CONTEXT: Platelet-rich plasma (PRP) accelerates bone union in vivo in a rodent model of spinal fusion surgery. However, PRP's effect on bone union after spinal surgery remains unclear. PURPOSE: The objective of this study was to evaluate the efficacy of PRP after posterolateral lumbar fusion (PLF) surgery. STUDY DESIGN/SETTING: Single-center prospective randomized controlled clinical trial with 2-year follow-up. PATIENT SAMPLE: The patient sample included a total 62 patients (31 patients in the PRP group or 31 patients in the control group)...
July 20, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28734116/preparing-platelet-rich-plasma-with-whole-blood-harvested-intraoperatively-during-spinal-fusion
#17
Bin Shen, Zheng Zhang, Ning-Feng Zhou, Yu-Feng Huang, Yu-Jie Bao, De-Sheng Wu, Ya-Dong Zhang
BACKGROUND Platelet-rich plasma (PRP) has gained growing popularity in use in spinal fusion procedures in the last decade. Substantial intraoperative blood loss is frequently accompanied with spinal fusion, and it is unknown whether blood harvested intraoperatively qualifies for PRP preparation. MATERIAL AND METHODS Whole blood was harvested intraoperatively and venous blood was collected by venipuncture. Then, we investigated the platelet concentrations in whole blood and PRP, the concentration of growth factors in PRP, and the effects of PRP on the proliferation and viability of human bone marrow-derived mesenchymal stem cells (HBMSCs)...
July 22, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28717879/comparison-of-the-effect-of-activated-or-non-activated-prp-in-various-concentrations-on-osteoblast-and-fibroblast-cell-line-proliferation
#18
Surena Vahabi, Zahra Yadegari, Hossein Mohammad-Rahimi
Platelet-rich plasma (PRP) contains growth factors which positively affect cell proliferation, cell differentiation, chemotaxis and intracellular matrix synthesis. All these processes are involved in wound healing and tissue regeneration; thus, PRP as a source of growth factors can be used in periodontal regenerative therapies. The purpose of the present study was to assess the effect of various concentrations of activated and non-activated PRP on proliferation of osteoblasts and fibroblasts in vitro. PRP was obtained from three healthy volunteers...
July 17, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28713711/the-role-of-human-adult-peripheral-and-umbilical-cord-blood-platelet-rich-plasma-on-proliferation-and-migration-of-human-skin-fibroblasts
#19
Seyedeh-Sara Hashemi, Mahdokht Mahmoodi, Ali Reza Rafati, Farzad Manafi, Davood Mehrabani
BACKGROUND: Wound healing is a complex and dynamic process following damage in tissue structures. Due to extensive skin damage caused by burn injuries, this study determined the role of human adult peripheral and umbilical cord blood platelet-rich plasma on proliferation and migration in human skin fibroblasts. METHODS: Platelet-rich plasma (5, 10, 15, 20 and 50% PRP) from human umbilical cord blood and adult peripheral blood were provided and added to fibroblasts cultured from a human skin sample...
May 2017: World Journal of Plastic Surgery
https://www.readbyqxmd.com/read/28706829/platelet-rich-plasma-augmentation-for-hip-arthroscopy
#20
Sandeep Mannava, Jorge Chahla, Andrew G Geeslin, Mark E Cinque, Kaitlyn E Whitney, Thos A Evans, Salvatore J Frangiamore, George LeBus, Jonathan Godin, Robert F LaPrade, Marc J Philippon
Biological augmentation and therapeutics are being increasingly used in musculoskeletal and orthopaedic care. Platelet-rich plasma (PRP) is produced from centrifugation of peripheral blood, a process that concentrates platelets within autologous plasma. The process of PRP preparation is fundamental in controlling the contents, and it influences its therapeutic potential. Platelets contain alpha granules that store and release a variety of growth factors and other proteins that may augment the healing environment; PRP also has the added benefit of promoting postsurgical hemostasis...
June 2017: Arthroscopy Techniques
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