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https://www.readbyqxmd.com/read/27904584/effectiveness-and-relevant-factors-of-platelet-rich-plasma-treatment-in-managing-plantar-fasciitis-a-systematic-review
#1
REVIEW
Seet Khing Chiew, Thamil Selvee Ramasamy, Farahnaz Amini
BACKGROUND: Plantar fasciitis (PF) is a common foot complaint, affects both active sportsmen and physically inactive middle age group. It is believed that PF results from degenerative changes rather than inflammation. Platelet-rich plasma (PRP) therapy has been introduced as an alternative therapy for PF. This study is aimed to systematically review to the effectiveness and relevant factors of PRP treatment in managing PF. MATERIALS AND METHODS: A search was conducted in electronic databases, including PubMed, Scopus, and Google Scholar using different keywords...
2016: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
https://www.readbyqxmd.com/read/27903591/platelet-rich-plasma-powder-a-new-preparation-method-for-the-standardization-of-growth-factor-concentrations
#2
Matthias Kieb, Frank Sander, Cornelia Prinz, Stefanie Adam, Anett Mau-Möller, Rainer Bader, Kirsten Peters, Thomas Tischer
BACKGROUND: Platelet-rich plasma (PRP) is widely used in sports medicine. Available PRP preparations differ in white blood cell, platelet, and growth factor concentrations, making standardized research and clinical application challenging. PURPOSE: To characterize a newly standardized procedure for pooled PRP that provides defined growth factor concentrations. STUDY DESIGN: Controlled laboratory study. METHODS: A standardized growth factor preparation (lyophilized PRP powder) was prepared using 12 pooled platelet concentrates (PCs) derived from different donors via apheresis...
November 30, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27900294/efficacy-of-autologous-leukocyte-reduced-platelet-rich-plasma-therapy-for-patellar-tendinopathy-in-a-rat-treadmill-model
#3
Mamoru Yoshida, Hiroki Funasaki, Keishi Marumo
BACKGROUND: An autologous platelet-rich plasma (PRP) therapy has currently been applied for the tendinopathy; however, its efficacy and an optimal platelets concentration in PRP were uncertain. We analyzed them in an animal model prepared using a repetitive running exercise. METHODS: We made the tendinopathy rat model of patellar tendon using a rodent treadmill machine. Rats with tendinopathy were injected with leukocyte-reduced PRP at the platelets concentration of 1...
April 2016: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/27900293/combined-use-of-platelet-rich-plasma-micro-fat-in-sport-and-race-horses-with-degenerative-joint-disease-preliminary-clinical-study-in-eight-horses
#4
Fabrizio Bembo, Julia Eraud, Cecile Philandrianos, Baptiste Bertrand, Alain Silvestre, Julie Veran, Florence Sabatier, Guy Magalon, Jeremy Magalon
BACKGROUND: To assess the safety and potential efficacy of a standardized technique consisting of intra-articular injection of 10 cc of a homogeneous mixed product using autologous micro-fat and platelet rich plasma (PRP) (ratio 1:1) in the carpus or the fetlock joint of sport horses presenting degenerative joint disease (DJD). METHODS: Eight sport horses with DJD confirmed by radiography and ultrasonography and causing lameness and the impossibility to compete were treated...
April 2016: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/27900155/normal-platelet-function-in-platelet-concentrates-requires-non-platelet-cells-a-comparative-in-vitro-evaluation-of-leucocyte-rich-type-1a-and-leucocyte-poor-type-3b-platelet-concentrates
#5
William R Parrish, Breana Roides, Julia Hwang, Michael Mafilios, Brooks Story, Samir Bhattacharyya
BACKGROUND: Therapeutic success of platelet-rich plasma (PRP) may vary based on the composition and preparation method. The objective of this study was to evaluate the cellular components of platelet concentrates produced by a leucocyte-rich (LR-PRP) and a leucocyte-poor PRP systems (LP-PRP). METHODS: Parameters evaluated included platelet recovery, platelet concentration, red blood cell (RBC) and white blood cell (WBC) composition, platelet growth factor release and stimulation of human tendon cell proliferation in vitro...
2016: BMJ Open Sport & Exercise Medicine
https://www.readbyqxmd.com/read/27900152/depa-classification-a-proposal-for-standardising-prp-use-and-a-retrospective-application-of-available-devices
#6
J Magalon, A L Chateau, B Bertrand, M L Louis, A Silvestre, L Giraudo, J Veran, F Sabatier
BACKGROUND/AIM: Significant biological differences in platelet-rich plasma (PRP) preparations have been highlighted and could explain the large variability in the clinical benefit of PRP reported in the literature. The scientific community now recommends the use of classification for PRP injection; however, these classifications are focused on platelet and leucocyte concentrations. This presents the disadvantages of (1) not taking into account the final volume of the preparation; (2) omitting the presence of red blood cells in PRP and (3) not assessing the efficiency of production...
2016: BMJ Open Sport & Exercise Medicine
https://www.readbyqxmd.com/read/27891436/platelet-rich-plasma-efficacy-in-treating-trophic-ulcers-in-leprosy
#7
V Anandan, W Afthab Jameela, P Saraswathy, S Sarankumar
INTRODUCTION: Trophic ulcers secondary to leprosy pose a great stigma to the patients and remain a challenge to the treating dermatologists. The discovery of Platelet Rich Plasma (PRP) with its favourable role in wound healing is a boon for the patients. PRP introduces the growth factors directly into the wound and aids in rapid healing. AIM: To study the efficacy and safety of PRP in the healing of trophic ulcers secondary to Hansen's disease in a tertiary care centre in Southern India...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27865782/application-of-nano-lc-maldi-tof-tof-ms-for-proteomic-analysis-of-microvesicles
#8
Joanna Kasprzyk, Ewa Stępień, Wojciech Piekoszewski
Activated platelets and platelet derived microvesicles (PMVs) emerged recently to be promising biomarkers. There is no universal procedure to carry out the proteomic analysis on microvesicles. In this study we proposed a nano-liquid chromatography (nano-LC) technique coupled off-line with a spectrometric measurement MALDI-TOF-MS/MS as a throughput and time-saving procedure. In this study we developed a simplified method to evaluate the protein composition of platelet organelles and PMVs. Platelet-rich plasma (PRP) was collected from healthy donors...
November 16, 2016: Clinical Biochemistry
https://www.readbyqxmd.com/read/27863057/platelet-rich-plasma-based-bioactive-membrane-as-a-new-advanced-wound-care-tool
#9
Raffaele Spanò, Anita Muraglia, Maria R Todeschi, Marta Nardini, Paolo Strada, Ranieri Cancedda, Maddalena Mastrogiacomo
Chronic skin ulcers, consequence of diabetes and other pathological conditions, heavily compromise the patients' life quality and represent a high and constantly growing cost for National Health Services. Autologous Platelet Rich Plasma (PRP), has been proposed to treat these lesions. The absence of guidelines for the PRP production and the need of a fresh preparation for each treatment lead us to develop a protocol for the production of an allogenic PRP based bioactive membrane (BAM), standardized for platelet concentration and growth factor release...
November 13, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27836542/anchorless-forms-of-prion-protein-impact-of-truncation-on-structure-destabilization-and-prion-protein-conversion
#10
Valerija Kovač, Iva Hafner-Bratkovič, Vladka Čurin Šerbec
Prion diseases are a group of fatal neurodegenerative diseases caused by scrapie form of prion protein, PrP(Sc). Prion protein (PrP) is bound to the cell via glycophosphatidylinositol (GPI) anchor. The role of GPI anchor in PrP(Sc) replication and propagation remains unclear. It has been shown that anchorless and truncated PrP accelerate the formation and propagation of prions in vivo and further increases the risk for transmission of prion diseases among species. To explain the role of anchorless forms of PrP in the development of prion diseases, we have prepared five C-terminal PrP truncated variants, determined their thermodynamic properties and analyzed the kinetics of conversion into amyloid fibrils...
December 2, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27833116/freeze-dried-platelet-rich-plasma-accelerates-bone-union-with-adequate-rigidity-in-posterolateral-lumbar-fusion-surgery-model-in-rats
#11
Yasuhiro Shiga, Sumihisa Orita, Go Kubota, Hiroto Kamoda, Masaomi Yamashita, Yusuke Matsuura, Kazuyo Yamauchi, Yawara Eguchi, Miyako Suzuki, Kazuhide Inage, Takeshi Sainoh, Jun Sato, Kazuki Fujimoto, Koki Abe, Hirohito Kanamoto, Masahiro Inoue, Hideyuki Kinoshita, Yasuchika Aoki, Tomoaki Toyone, Takeo Furuya, Masao Koda, Kazuhisa Takahashi, Seiji Ohtori
Fresh platelet-rich plasma (PRP) accelerates bone union in rat model. However, fresh PRP has a short half-life. We suggested freeze-dried PRP (FD-PRP) prepared in advance and investigated its efficacy in vivo. Spinal posterolateral fusion was performed on 8-week-old male Sprague-Dawley rats divided into six groups based on the graft materials (n = 10 per group): sham control, artificial bone (A hydroxyapatite-collagen composite) -alone, autologous bone, artificial bone + fresh-PRP, artificial bone + FD-PRP preserved 8 weeks, and artificial bone + human recombinant bone morphogenetic protein 2 (BMP) as a positive control...
November 11, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27808552/separating-limits-on-preparation-versus-online-processing-in-multitasking-paradigms-evidence-for-resource-models
#12
Victor Mittelstädt, Jeff Miller
We conducted 2 multitasking experiments to examine the finding that first-task reaction times (RTs) are slower in the psychological refractory period (PRP) paradigm than in the prioritized processing (PP) paradigm. To see whether this difference between the 2 paradigms could be explained entirely by differences in first-task preparation, which would be consistent with the standard response selection bottleneck (RSB) model for multitasking interference, we compared the size of this difference for trials in which a second-task stimulus actually occurred against the size of the difference for trials without any second-task stimulus...
November 3, 2016: Journal of Experimental Psychology. Human Perception and Performance
https://www.readbyqxmd.com/read/27805447/evaluation-of-two-platelet-rich-plasma-processing-methods-and-two-platelet-activation-techniques-for-use-in-llamas-and-alpacas
#13
Stacy A Semevolos, Cori D Youngblood, Stephanie K Grissom, M Elena Gorman, Maureen K Larson
OBJECTIVE To evaluate 2 processing methods (commercial kit vs conical tube centrifugation) for preparing platelet rich plasma (PRP) for use in llamas and alpacas. SAMPLES Blood samples (30 mL each) aseptically collected from 6 healthy llamas and 6 healthy alpacas. PROCEDURES PRP was prepared from blood samples by use of a commercial kit and by double-step conical tube centrifugation. A CBC was performed for blood and PRP samples. Platelets in PRP samples were activated by means of a freeze-thaw method with or without 23mM CaCl2, and concentrations of platelet-derived growth factor-BB and transforming growth factor-β1 were measured...
November 2016: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/27797334/-liposomal-form-of-lipoic-acid-preparation-and-determination-of-antiplatelet-and-antioxidant-activity
#14
V A Shchelkonogov, G M Sorokoumova, O A Baranova, A V Chekanov, A V Klochkova, K D Kazarinov, E Y Solovieva, A I Fedin, V I Shvets
Optimal conditions for obtaining phosphatidylholine (PC) liposomes with lipoic acid (LA) are chosen that lead to the formation of nanoparticles with a size of 175¸284 nm with efficiency (extent) of inclusion of LA in liposomes equal 85% and characterized by a slow release of substance from the nanoparticles. The effect of "empty" liposomes and liposomal form of LA on platelet aggregation induced by arachidonic acid (AA) is established. It is found that liposomes with LА inhibit platelet aggregation, caused by AА, to 80%...
July 2016: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
https://www.readbyqxmd.com/read/27791406/reconstruction-of-cranial-cruciate-ligament-in-rabbits-using-polyester-implants-saturated-with-prp-antlerogenic-stem-cells-mic-1-and-their-homogenate
#15
Przemysław Prządka, Zdzisław Kiełbowicz, Bogdan Osiński, Stanisław Dzimira, Janusz A Madej, Wojciech Nowacki, Krzysztof Kubiak, Paweł Reichert, Marek Cegielski
AIM OF THE STUDY: The attempt to limit the negative effects of polyester implants on the articular cavity by using preparations containing growth factors. MATERIALS AND METHODS: Polyester implants used for the reconstruction of a rabbit's cranial cruciate ligament (CCL) were saturated with autogenic platelet-rich plasma, antlerogenic stem cells MIC-1 and their homogenate prior to the surgery. Six months after CCL reconstruction, morphological and biochemical blood tests were carried out, including proteinogram and acute phase proteins...
October 28, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27788903/platelet-rich-plasma
#16
REVIEW
Peter I-Kung Wu, Robert Diaz, Joanne Borg-Stein
Platelet-rich plasma (PRP) is a growing and robust therapeutic option in musculoskeletal medicine. PRP is a preparation of autologous plasma enriched with a platelet concentration above that normally contained in whole blood. The rationale for use and therapeutic potential of a high concentration of platelets is based on their capacity to supply supraphysiologic amounts of essential growth factors to provide a regenerative stimulus that promotes repair in tissues with low healing potential. This article reviews the latest basic science on PRP, clinical evidence for its use in musculoskeletal medicine, limitations in current knowledge, and critical areas for future research...
November 2016: Physical Medicine and Rehabilitation Clinics of North America
https://www.readbyqxmd.com/read/27771585/efficacy-of-freeze-dried-platelet-rich-plasma-in-bone-engineering
#17
Yuya Nakatani, Hideki Agata, Yoshinori Sumita, Takamitsu Koga, Izumi Asahina
OBJECTIVE: Platelet-rich plasma (PRP) is typically isolated and applied immediately after preparation, making it both a time- and labor-intensive addition to the operative procedure. Thus, it would be convenient if PRP could be preserved. We evaluated the efficacy of freeze-dried PRP (FD-PRP), as compared with freshly isolated PRP (f-PRP) for bone engineering. DESIGN: FD-PRP was prepared by lyophilization of f-PRP and was subsequently preserved at -20°C for one month...
October 15, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27747711/growth-factor-and-pro-inflammatory-cytokine-contents-in-platelet-rich-plasma-prp-plasma-rich-in-growth-factors-prgf-advanced-platelet-rich-fibrin-a-prf-and-concentrated-growth-factors-cgf
#18
Hideo Masuki, Toshimitsu Okudera, Taisuke Watanebe, Masashi Suzuki, Kazuhiko Nishiyama, Hajime Okudera, Koh Nakata, Kohya Uematsu, Chen-Yao Su, Tomoyuki Kawase
BACKGROUND: The development of platelet-rich fibrin (PRF) drastically simplified the preparation procedure of platelet-concentrated biomaterials, such as platelet-rich plasma (PRP), and facilitated their clinical application. PRF's clinical effectiveness has often been demonstrated in pre-clinical and clinical studies; however, it is still controversial whether growth factors are significantly concentrated in PRF preparations to facilitate wound healing and tissue regeneration. To address this matter, we performed a comparative study of growth factor contents in PRP and its derivatives, such as advanced PRF (A-PRF) and concentrated growth factors (CGF)...
December 2016: Int J Implant Dent
https://www.readbyqxmd.com/read/27747653/in-vitro-immunological-and-biological-evaluations-of-the-angiogenic-potential-of-platelet-rich-fibrin-preparations-a-standardized-comparison-with-prp-preparations
#19
Mito Kobayashi, Tomoyuki Kawase, Kazuhiro Okuda, Larry F Wolff, Hiromasa Yoshie
BACKGROUND: Platelet-rich fibrin (PRF), a platelet-rich plasma (PRP) derivative mainly composed of fibrin networks, has been increasingly demonstrated to be effective in wound healing in clinical and pre-clinical animal studies. However, there has still been a concern that major growth factors may significantly be loss from PRF during its preparation through the slow clotting process. To address this concern, we compared the angiogenic potential of PRF and PRP by standardization of procedures based on volume ratios...
December 2015: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/27735933/the-real-time-quaking-induced-conversion-assay-for-detection-of-human-prion-disease-and-study-of-other-protein-misfolding-diseases
#20
Matthias Schmitz, Maria Cramm, Franc Llorens, Dominik Müller-Cramm, Steven Collins, Ryuichiro Atarashi, Katsuya Satoh, Christina D Orrù, Bradley R Groveman, Saima Zafar, Walter J Schulz-Schaeffer, Byron Caughey, Inga Zerr
The development and adaption of in vitro misfolded protein amplification systems has been a major innovation in the detection of abnormally folded prion protein scrapie (PrP(Sc)) in human brain and cerebrospinal fluid (CSF) samples. Herein, we describe a fast and efficient protein amplification technique, real-time quaking-induced conversion (RT-QuIC), for the detection of a PrP(Sc) seed in human brain and CSF. In contrast to other in vitro misfolded protein amplification assays-such as protein misfolding cyclic amplification (PMCA)-which are based on sonication, the RT-QuIC technique is based on prion seed-induced misfolding and aggregation of recombinant prion protein substrate, accelerated by alternating cycles of shaking and rest in fluorescence plate readers...
November 2016: Nature Protocols
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