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https://www.readbyqxmd.com/read/28735763/platelet-rich-plasma-enhances-bone-union-in-posterolateral-lumbar-fusion-a-prospective-randomized-controlled-trial
#1
Go Kubota, Hiroto Kamoda, Sumihisa Orita, Kazuyo Yamauchi, Yoshihiro Sakuma, Yasuhiro Oikawa, Kazuhide Inage, Takeshi Sainoh, Jun Sato, Michihiro Ito, Masaomi Yamashita, Junichi Nakamura, Takane Suzuki, Kazuhisa Takahashi, Seiji Ohtori
BACKGROUND CONTEXT: Platelet-rich plasma (PRP) accelerates bone union in vivo in a rodent model of spinal fusion surgery. However, PRP's effect on bone union after spinal surgery remains unclear. PURPOSE: To evaluate the efficacy of PRP after posterolateral lumbar fusion (PLF) surgery. STUDY DESIGN/SETTING: Single-center prospective randomized controlled clinical trial with 2-year follow-up. PATIENT SAMPLE: Total 62 patients (31 patients in PRP group or 31 patients in control groups OUTCOME MEASURES: Bone fusion rate, area of bone fusion mass, duration for bone fusion, and clinical score using visual analog scale (VAS)...
July 20, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28734116/preparing-platelet-rich-plasma-with-whole-blood-harvested-intraoperatively-during-spinal-fusion
#2
Bin Shen, Zheng Zhang, Ning-Feng Zhou, Yu-Feng Huang, Yu-Jie Bao, De-Sheng Wu, Ya-Dong Zhang
BACKGROUND Platelet-rich plasma (PRP) has gained growing popularity in use in spinal fusion procedures in the last decade. Substantial intraoperative blood loss is frequently accompanied with spinal fusion, and it is unknown whether blood harvested intraoperatively qualifies for PRP preparation. MATERIAL AND METHODS Whole blood was harvested intraoperatively and venous blood was collected by venipuncture. Then, we investigated the platelet concentrations in whole blood and PRP, the concentration of growth factors in PRP, and the effects of PRP on the proliferation and viability of human bone marrow-derived mesenchymal stem cells (HBMSCs)...
July 22, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28717879/comparison-of-the-effect-of-activated-or-non-activated-prp-in-various-concentrations-on-osteoblast-and-fibroblast-cell-line-proliferation
#3
Surena Vahabi, Zahra Yadegari, Hossein Mohammad-Rahimi
Platelet-rich plasma (PRP) contains growth factors which positively affect cell proliferation, cell differentiation, chemotaxis and intracellular matrix synthesis. All these processes are involved in wound healing and tissue regeneration; thus, PRP as a source of growth factors can be used in periodontal regenerative therapies. The purpose of the present study was to assess the effect of various concentrations of activated and non-activated PRP on proliferation of osteoblasts and fibroblasts in vitro. PRP was obtained from three healthy volunteers...
July 17, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28713711/the-role-of-human-adult-peripheral-and-umbilical-cord-blood-platelet-rich-plasma-on-proliferation-and-migration-of-human-skin-fibroblasts
#4
Seyedeh-Sara Hashemi, Mahdokht Mahmoodi, Ali Reza Rafati, Farzad Manafi, Davood Mehrabani
BACKGROUND: Wound healing is a complex and dynamic process following damage in tissue structures. Due to extensive skin damage caused by burn injuries, this study determined the role of human adult peripheral and umbilical cord blood platelet-rich plasma on proliferation and migration in human skin fibroblasts. METHODS: Platelet-rich plasma (5, 10, 15, 20 and 50% PRP) from human umbilical cord blood and adult peripheral blood were provided and added to fibroblasts cultured from a human skin sample...
May 2017: World Journal of Plastic Surgery
https://www.readbyqxmd.com/read/28706829/platelet-rich-plasma-augmentation-for-hip-arthroscopy
#5
Sandeep Mannava, Jorge Chahla, Andrew G Geeslin, Mark E Cinque, Kaitlyn E Whitney, Thos A Evans, Salvatore J Frangiamore, George LeBus, Jonathan Godin, Robert F LaPrade, Marc J Philippon
Biological augmentation and therapeutics are being increasingly used in musculoskeletal and orthopaedic care. Platelet-rich plasma (PRP) is produced from centrifugation of peripheral blood, a process that concentrates platelets within autologous plasma. The process of PRP preparation is fundamental in controlling the contents, and it influences its therapeutic potential. Platelets contain alpha granules that store and release a variety of growth factors and other proteins that may augment the healing environment; PRP also has the added benefit of promoting postsurgical hemostasis...
June 2017: Arthroscopy Techniques
https://www.readbyqxmd.com/read/28692513/viability-and-biomechanics-of-diced-cartilage-blended-with-platelet-rich-plasma-and-wrapped-with-poly-lactic-co-glycolic-acid-membrane
#6
Jun-Lin Liao, Jia Chen, Bin He, Yong Chen, Jia-Qun Xu, Hong-Ju Xie, Feng Hu, Ai-Jun Wang, ChengQun Luo, Qing-Feng Li, Jian-Da Zhou
OBJECTIVES: The objective of this study was to investigate the viability and biomechanics of diced cartilage blended with platelet-rich plasma (PRP) and wrapped with poly (lactic-co-glycolic) acid (PLGA) membrane in a rabbit model. METHODS: A total of 10 New Zealand rabbits were used for the study. Cartilage grafts were harvested from 1 side ear. The grafts were divided into 3 groups for comparison: bare diced cartilage, diced cartilage wrapped with PLGA membrane, and diced cartilage blended with PRP and wrapped with PLGA membrane...
July 7, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28681651/the-contribution-of-leucocytes-to-the-antimicrobial-activity-of-platelet-rich-plasma-preparations-a-systematic-review
#7
Federica D'asta, Fenella Halstead, Paul Harrison, Sandra Zecchi Orlandini, Naiem Moiemen, Janet Lord
The infection of a wound is one of the major contributors to delays in healing and tissue regeneration. As multi-drug resistance to antibiotics is becoming a serious threat, research in this field has focused on finding new agents and strategies to fight infection and additionally to reduce healing times. The topical use of autologous Platelet Rich Plasma (PRP) as a biological accelerator of the healing process, has been safely used as a form of treatment for wounds since the 1990s. Although the presence or absence of leucocytes in PRP preparation was previously neglected, in the last decade more attention has been paid to their role and several studies have been conducted to explore both their immuno-metabolic effects and their antimicrobial properties...
July 6, 2017: Platelets
https://www.readbyqxmd.com/read/28670400/freeze-dried-human-platelet-rich-plasma-retains-activation-and-growth-factor-expression-after-an-eight-week-preservation-period
#8
Yasuhiro Shiga, Go Kubota, Sumihisa Orita, Kazuhide Inage, Hiroto Kamoda, Masaomi Yamashita, Toru Iseki, Michihiro Ito, Kazuyo Yamauchi, Yawara Eguchi, Takeshi Sainoh, Jun Sato, Kazuki Fujimoto, Koki Abe, Hirohito Kanamoto, Masahiro Inoue, Hideyuki Kinoshita, Takeo Furuya, Masao Koda, Yasuchika Aoki, Tomoaki Toyone, Kazuhisa Takahashi, Seiji Ohtori
STUDY DESIGN: Controlled laboratory study. PURPOSE: This study aimed to evaluate the efficacy of platelet-rich plasma (PRP) stored at room temperature (RT), frozen, or after freeze-drying. OVERVIEW OF LITERATURE: PRP enriches tissue repair and regeneration, and is a novel treatment option for musculoskeletal pathologies. However, whether biological activity is preserved during PRP storage remains uncertain. METHODS: PRP was prepared from blood of 12 healthy human volunteers (200 mL/person) and stored using three methods: PRP was stored at RT with shaking, PRP was frozen and stored at -80℃, or PRP was freeze-dried and stored at RT...
June 2017: Asian Spine Journal
https://www.readbyqxmd.com/read/28662879/use-of-platelet-rich-plasma-in-endodontic-procedures-in-adults-regeneration-or-repair-a-report-of-3-cases-with-5%C3%A2-years-of-follow-up
#9
José Francisco Gaviño Orduña, Javier Caviedes-Bucheli, María C Manzanares Céspedes, Esther Berástegui Jimeno, Benjamín Martín Biedma, Juan José Segura-Egea, José López-López
INTRODUCTION: Regenerative endodontic procedures (REP) are a promising alternative for necrotic immature teeth in adolescents and children, but very little evidence is available on this alternative in long-lasting necrotic teeth with open apices in adults. REPs are designed to replace damaged structures of the pulp-dentin complex, but no regeneration has been obtained in any of the cases described in necrotic immature permanent teeth with apical periodontitis with histologic results. The results are limited to periapical tissue repair with increasing root length, thickening of the root walls, and apical closure in young patients...
June 26, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28651609/optimisation-of-a-double-centrifugation-method-for-preparation-of-canine-platelet-rich-plasma
#10
Hyeok-Soo Shin, Heung-Myong Woo, Byung-Jae Kang
BACKGROUND: Platelet-rich plasma (PRP) has been expected for regenerative medicine because of its growth factors. However, there is considerable variability in the recovery and yield of platelets and the concentration of growth factors in PRP preparations. The aim of this study was to identify optimal relative centrifugal force and spin time for the preparation of PRP from canine blood using a double-centrifugation tube method. METHODS: Whole blood samples were collected in citrate blood collection tubes from 12 healthy beagles...
June 26, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28643541/effects-of-ph-and-concentration-of-sodium-citrate-anticoagulant-on-platelet-aggregation-measured-by-light-transmission-aggregometry-induced-by-adenosine-diphosphate
#11
Ksenia Germanovich, Eti Alessandra Femia, Chun Yan Cheng, Natalia Dovlatova, Marco Cattaneo
The 2013 ISTH-SSC guidelines for the standardization of light transmission aggregometry (LTA) were largely based on expert consensus, as studies directly comparing LTA methodologies were lacking. We experimentally tested the cogency of ISTH-SSC recommendations pertaining to use of anticoagulant, in particular whether: (1) buffered citrate (BC) is preferable to unbuffered citrate (C); (2) the two recommended concentrations of sodium citrate (109 and 129 mM) are equivalent in terms of platelet aggregation (PA)...
June 23, 2017: Platelets
https://www.readbyqxmd.com/read/28643538/do-methodological-differences-account-for-the-current-controversy-on-tissue-factor-expression-in-platelets
#12
Marta Brambilla, Laura Rossetti, Chiara Zara, Paola Canzano, Peter L A Giesen, Elena Tremoli, Marina Camera
Tissue factor (TF), the key activator of the blood coagulation cascade and of thrombus formation, is also expressed by circulating human platelets. Despite the documented in-depth characterization of platelet TF carried out in the past 15 years, some authors still fail to identify TF in platelets, especially when assessment in platelet-rich plasma (PRP) or washed platelets is carried out. This study aims to extend the characterization of the subset of TF-positive platelets in PRP from healthy subjects and to verify how different centrifugation forces, used to prepare the PRP, could affect the analysis of TF-positive platelets...
June 23, 2017: Platelets
https://www.readbyqxmd.com/read/28639068/what-about-the-rheological-properties-of%C3%A2-prp-microfat-mixtures-in-fat-grafting-procedure
#13
R Ghazouane, B Bertrand, C Philandrianos, J Veran, M Abellan, P Francois, M Velier, C Orneto, P Piccerelle, J Magalon
BACKGROUND: Fat grafting has emerged as a reference procedure in daily plastic surgery practice. Unpredictable fat resorption is the main clinical problem. For this purpose, the addition of PRP to enhance fat revascularization is now an easy and popular procedure. However, no consensus exists regarding the respective volume of fat and PRP used to obtain the ideal mixture. This study investigated the rheological properties of microfat mixed with different proportions of PRP. Results obtained were compared with commercialized hyaluronic acid fillers...
June 21, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28638679/decrease-of-serum-biomarker-of-type-ii-collagen-degradation-coll2-1-by-intra-articular-injection-of-an-autologous-plasma-rich-platelet-in-patients-with-unilateral-primary-knee-osteoarthritis
#14
Rasha Mohamed Fawzy, Nashwa Ismail Hashaad, Amira Ibrahim Mansour
OBJECTIVE: This study aimed to evaluate the effect of one dose of intra-articular injection of (PRP) in the knee joint on a specific osteoarthritis (OA) serum biomarker of cartilage degeneration, Collagen 2-1 (Coll2-1), over a short period of 3 months. The aim extended to clarify the effect of PRP on the functional status of the osteoarthritic knee joint. MATERIAL AND METHODS: Sixty patients with primary unilateral knee OA were enrolled in this study. They were subdivided according to Kellgren-Lawrence grading scale (KL) into (Group I): including patients with KL grade < 3 and (Group II): including patients with KL grade ≥3...
June 2017: European Journal of Rheumatology
https://www.readbyqxmd.com/read/28629420/comparison-of-the-effects-of-platelet-rich-or-growth-factor-rich-plasma-on-intestinal-anastomosis-healing-in-pigs
#15
Gessica Giusto, Cristina Vercelli, Selina Iussich, Massimiliano Tursi, Giovanni Perona, Marco Gandini
BACKGROUND: The use of autologous platelet-rich plasma (PRP) and plasma rich in growth factors (PRGF) has been proposed for the treatment of several acute and chronic syndromes, such as corneal epithelial defects and dry eye syndrome, gum bleeding during oral surgery, and in orthopaedic surgery. We hypothesized that PRGF, rather than PRP, could be more effective because of its intrinsic characteristics in promoting the healing of intestinal anastomosis. The purpose of the present study was to evaluate and compare the effects of PRP and PRGF on various parameters of anastomotic healing in a swine model...
June 19, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28610547/human-plasma-rich-in-growth-factors-improves-survival-and-neurite-outgrowth-of-spiral-ganglion-neurons-in-vitro
#16
Michael Stolle, Jennifer Schulze, Ariane Römer, Thomas Lenarz, Martin Durisin, Athanasia Warnecke
BACKGROUND: Platelet-rich and platelet poor plasma (PRP and PPP) are autologous preparations from peripheral blood and contain several growth factors and cytokines involved in tissue repair. Although their neuroprotective and -regenerative properties have been already described, little is known about their effects in the inner ear. We therefore examined the effects of PRP and PPP on spiral ganglion neurons (SGN) in vitro. RESULTS: For all experiments, spiral ganglia were isolated from neonatal rats and were cultured in serum-free medium...
June 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28602130/a-novel-method-for-the-activation-of-platelet-rich-plasma-via-bead-mill-homogenizer-for-mesenchymal-stem-cell-culture
#17
Hyunwook Myung, Hyosun Jang, Jae Kyung Myung, Min-Jung Kim, Seung Bum Lee, Won-Suk Jang, Sun-Joo Lee, Hwi-Yool Kim, Seung-Sook Lee, Sehwan Shim, Sunhoo Park
Activated platelet-rich plasma (PRP) has been studied as a replacement for fetal bovine serum (FBS) in stem cell culture. However, current methods are time-consuming or require addition of exogenous substances to activate PRP, which have disadvantages in clinical applications. In this study, we developed a new method for PRP activation using a bead mill homogenizer and compared it with previous methods of PRP activation. PRP was prepared via a two-step centrifugation process and activated via calcium (Ca-PRP), freeze-thaw cycles (FT-PRP), or bead mill homogenizer processing (BM-PRP)...
June 10, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28590091/an-update-on-the-protocols-and-biologic-actions-of-platelet-rich-fibrin-in-dentistry
#18
Rucha Shah, Triveni M G, Raison Thomas, Dhoom Singh Mehta
Platelet rich fibrin (PRF) is a surgical biologic additive that is prepared by manipulation of autologous blood. It has now evolved to become one of the most widely used platelet concentrate in dentistry. It has almost replaced Platelet rich plasma (PRP) for usage owing to its advantages such as being 100% autogenous, easy technique, time and cost effectiveness, superior & prolonged growth factor release. It finds varied applications in dentistry including management of gingival recession, for guided bone regeneration in periodontal, peri-implant and endodontic bone defects...
June 2017: European Journal of Prosthodontics and Restorative Dentistry
https://www.readbyqxmd.com/read/28560233/evolution-current-status-and-advances-in-application-of-platelet-concentrate-in-periodontics-and-implantology
#19
REVIEW
Amit Arvind Agrawal
Platelet concentrates (PC) [platelet-rich plasma (PRP) and platelet-rich fibrin (PRF)] are frequently used for surgical procedures in medical and dental fields, particularly in oral and maxillofacial surgery, plastic surgery and sports medicine. The objective of all these technologies is to extract all the elements from a blood sample that could be used to improve healing and promote tissue regeneration. Although leukocyte rich and leukocyte poor PRP's have their own place in literature, the importance of non-platelet components in a platelet concentrate remains a mystery...
May 16, 2017: World Journal of Clinical Cases
https://www.readbyqxmd.com/read/28511520/effect-of-trauma-to-primary-tooth-on-permanent-maxillary-incisors-a-clinical-case-report
#20
Bhawana Gupta, Sachin Gupta, Jitesh Wadhwa, Alpa Gupta
Trauma to primary tooth leads to abnormal variations in crown and root canals of permanent teeth that presents a challenge in diagnosis and clinical management to the practitioner. This article presents a detailed case report of the endodontic treatment of a three canalled maxillary central incisor and maxillary lateral incisor having single canal with open apex linked with periodontal defect. Root canals were prepared with hand files and canals were irrigated with 2.5% sodium hypochlorite solution. Calcium hydroxide was placed as an intracanal medicament...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
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