keyword
MENU ▼
Read by QxMD icon Read
search

mtDNA

keyword
https://www.readbyqxmd.com/read/28214560/mitochondrial-genotype-modulates-mtdna-copy-number-and-organismal-phenotype-in-drosophila
#1
Tiina S Salminen, Marcos T Oliveira, Giuseppe Cannino, Päivi Lillsunde, Howard T Jacobs, Laurie S Kaguni
We evaluated the role of natural mitochondrial DNA (mtDNA) variation on mtDNA copy number, biochemical features and life history traits in Drosophila cybrid strains. We demonstrate the effects of both coding region and non-coding A+T region variation on mtDNA copy number, and demonstrate that copy number correlates with mitochondrial biochemistry and metabolically important traits such as development time. For example, high mtDNA copy number correlates with longer development times. Our findings support the hypothesis that mtDNA copy number is modulated by mtDNA genome variation and suggest that it affects OXPHOS efficiency through changes in the organization of the respiratory membrane complexes to influence organismal phenotype...
February 15, 2017: Mitochondrion
https://www.readbyqxmd.com/read/28212874/multilocus-phylogeny-of-alligator-lizards-elgaria-anguidae-testing-mtdna-introgression-as-the-source-of-discordant-molecular-phylogenetic-hypotheses
#2
Dean H Leavitt, Angela B Marion, Bradford D Hollingsworth, Tod W Reeder
The increased availability of nuclear DNA sequence data has led to a better appreciation of the role and frequency of introgressive hybridization and subsequent mitochondrial capture in misleading phylogenetic hypotheses based on mtDNA sequence data alone. Relationships among members of the alligator lizard genus Elgaria have been addressed with morphology, allozyme and mtDNA sequence data with discordant results. In this study, we use seven nuclear loci (total of 5.9 kb) and ∼3 kb of mtDNA to infer the phylogenetic relationships among Elgaria species and test whether the discordance among previous phylogenetic hypotheses is due to introgression and mtDNA capture...
February 14, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28209927/improved-health-span-and-lifespan-in-mtdna-mutator-mice-treated-with-the-mitochondrially-targeted-antioxidant-skq1
#3
Irina G Shabalina, Mikhail Yu Vyssokikh, Natalia Gibanova, Robert I Csikasz, Daniel Edgar, Anne Hallden-Waldemarson, Zinaida Rozhdestvenskaya, Lora E Bakeeva, Valeria B Vays, Antonina V Pustovidko, Maxim V Skulachev, Barbara Cannon, Vladimir P Skulachev, Jan Nedergaard
MtDNA mutator mice exhibit marked features of premature aging. We find that these mice treated from age of ≈100 days with the mitochondria-targeted antioxidant SkQ1 showed a delayed appearance of traits of aging such as kyphosis, alopecia, lowering of body temperature, body weight loss, as well as ameliorated heart, kidney and liver pathologies. These effects of SkQ1 are suggested to be related to an alleviation of the effects of an enhanced reactive oxygen species (ROS) level in mtDNA mutator mice: the increased mitochondrial ROS released due to mitochondrial mutations probably interact with polyunsaturated fatty acids in cardiolipin, releasing malondialdehyde and 4-hydroxynonenal that form protein adducts and thus diminishes mitochondrial functions...
February 15, 2017: Aging
https://www.readbyqxmd.com/read/28209105/navajo-neurohepatopathy%C3%A2-a-case-report-and-literature-review-emphasizing-clinicopathologic-diagnosis
#4
REVIEW
C P Bitting, J A Hanson
Navajo Neurohepatopathy (NNH) is a rare hepatocerebral mitochondrial DNA (mtDNA) depletion syndrome (MDS) with nonspecific clinical or pathologic features aside from Navajo ancestry. Because of the rarity of NNH, diagnosis rests on close clinicopathologic correlation and appropriate tissue triage for quantitative mtDNA analysis. We present a new case of NNH in which the clinical presentation and H&E liver biopsy histology indicated the need for NNH workup. Quantitative analysis of mtDNA in liver tissue was significantly reduced, and mutational analysis of the MPV17 gene confirmed homozygosity for the NNH-associated missense mutation, R50Q...
September 2016: Acta Gastro-enterologica Belgica
https://www.readbyqxmd.com/read/28208701/excitotoxins-mitochondrial-and-redox-disturbances-in-multiple-sclerosis
#5
REVIEW
Cecilia Rajda, Dániel Pukoli, Zsuzsanna Bende, Zsófia Majláth, László Vécsei
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS). There is increasing evidence that MS is not only characterized by immune mediated inflammatory reactions, but also by neurodegenerative processes. There is cumulating evidence that neurodegenerative processes, for example mitochondrial dysfunction, oxidative stress, and glutamate (Glu) excitotoxicity, seem to play an important role in the pathogenesis of MS. The alteration of mitochondrial homeostasis leads to the formation of excitotoxins and redox disturbances...
February 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28207832/towards-sustainable-fishery-management-for-skates-in-south-america-the-genetic-population-structure-of-zearaja-chilensis-and-dipturus-trachyderma-chondrichthyes-rajiformes-in-the-south-east-pacific-ocean
#6
Carolina Vargas-Caro, Carlos Bustamante, Michael B Bennett, Jennifer R Ovenden
The longnose skates (Zearaja chilensis and Dipturus trachyderma) are the main component of the elasmobranch fisheries in the south-east Pacific Ocean. Both species are considered to be a single stock by the fishery management in Chile however, little is known about the level of demographic connectivity within the fishery. In this study, we used a genetic variation (560 bp of the control region of the mitochondrial genome and ten microsatellite loci) to explore population connectivity at five locations along the Chilean coast...
2017: PloS One
https://www.readbyqxmd.com/read/28207748/nucleotide-pools-dictate-the-identity-and-frequency-of-ribonucleotide-incorporation-in-mitochondrial-dna
#7
Anna-Karin Berglund, Clara Navarrete, Martin K M Engqvist, Emily Hoberg, Zsolt Szilagyi, Robert W Taylor, Claes M Gustafsson, Maria Falkenberg, Anders R Clausen
Previous work has demonstrated the presence of ribonucleotides in human mitochondrial DNA (mtDNA) and in the present study we use a genome-wide approach to precisely map the location of these. We find that ribonucleotides are distributed evenly between the heavy- and light-strand of mtDNA. The relative levels of incorporated ribonucleotides reflect that DNA polymerase γ discriminates the four ribonucleotides differentially during DNA synthesis. The observed pattern is also dependent on the mitochondrial deoxyribonucleotide (dNTP) pools and disease-causing mutations that change these pools alter both the absolute and relative levels of incorporated ribonucleotides...
February 16, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28206858/genetic-polymorphism-of-aedes-albopictus-population-inferred-from-nd5-gene-variabilities-in-subang-jaya-malaysia
#8
Nurul Adilah-Amrannudin, Mayamin Hamsidi, Nurul-Ain Ismail, Rodziah Ismail, Nazri Che Dom, Abu Hassan Ahmad, Mohd Fahmi Mastuki, Tengku Shahrul Anuar Tengku Ahmad Basri, Adira Khalid, Mohammad Muslim, Nurul Amalina Ahmad Daud, Siti Nazrina Camalxaman
This study was performed to establish the genetic variability of Aedes albopictus within Subang Jaya, Selangor, Malaysia, by using the nicotinamide adenine dinucleotide dehydrogenase 5 subunit (ND5) mitochondrial DNA (mtDNA) marker. A total of 90 samples were collected from 9 localities within an area of the Subang Jaya Municipality. Genetic variability was determined through the amplification and sequencing of a fragment of the ND5 gene. Eight distinct mtDNA haplotypes were identified. The evolutionary relationship of the local haplotypes alongside 28 reference strains was used to construct a phylogram, the analysis of which revealed low genetic differentiation in terms of both nucleotide and haplotype diversity...
December 2016: Journal of the American Mosquito Control Association
https://www.readbyqxmd.com/read/28199844/mr-1s-interacts-with-pet100-and-pet117-in-module-based-assembly-of-human-cytochrome-c-oxidase
#9
Sara Vidoni, Michael E Harbour, Sergio Guerrero-Castillo, Alba Signes, Shujing Ding, Ian M Fearnley, Robert W Taylor, Valeria Tiranti, Susanne Arnold, Erika Fernandez-Vizarra, Massimo Zeviani
The biogenesis of human cytochrome c oxidase (COX) is an intricate process in which three mitochondrial DNA (mtDNA)-encoded core subunits are assembled in a coordinated way with at least 11 nucleus-encoded subunits. Many chaperones shared between yeast and humans are involved in COX assembly. Here, we have used a MT-CO3 mutant cybrid cell line to define the composition of assembly intermediates and identify new human COX assembly factors. Quantitative mass spectrometry analysis led us to modify the assembly model from a sequential pathway to a module-based process...
February 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/28199200/mitochondrial-dna-mitochondrial-dysfunction-and-cardiac-manifestations
#10
Sung Ryul Lee, Nari Kim, Yeun Hee Noh, Zhelong Xu, Kyung Soo Ko, Byoung Doo Rhee, Jin Han
Mitochondria, are the powerhouses of cells, have their own DNA (mtDNA), regulate the transport of metabolites and ions, and impact cell physiology, survival, and death. Mitochondrial dysfunction, including impaired oxidative phosphorylation, preferentially affects heart function due to an imbalance of energy supply and demand. Recently, mitochondrial mutations and associated mitochondrial dysfunction were suggested as a causal factor of cardiac manifestations. Oxidative stress largely influences mtDNA stability due to oxidative modifications of mtDNA...
March 1, 2017: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/28199194/regulation-of-mitochondrial-gene-expression-the-epigenetic-enigma
#11
Archibold Mposhi, Monique Gp Van der Wijst, Klaas Nico Faber, Marianne G Rots
Epigenetics provides an important layer of information on top of the DNA sequence and is essential for establishing gene expression profiles. Extensive studies have shown that nuclear DNA methylation and histone modifications influence nuclear gene expression. However, it remains unclear whether mitochondrial DNA (mtDNA) undergoes similar epigenetic changes to regulate mitochondrial gene expression. Recently, it has been shown that mtDNA is differentially methylated in various diseases such as diabetes and colorectal cancer...
March 1, 2017: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/28198717/mitochondrial-dna-induced-inflammatory-responses-and-lung-injury-in-thermal-injury-rat-model-protective-effect-of-epigallocatechin-gallate
#12
Ruiqi Liu, Fei Xu, Si Si, Xueshan Zhao, Siwei Bi, Ying Cen
Lungs are easily damaged by the inflammatory responses induced after extensive burns. The aim here was to investigate the protective role of epigallocatechin gallate (EGCG) in mitochondrial DNA (mtDNA)-mediated inflammatory responses and acute respiratory distress syndrome (ARDS) in a rat model of thermal injury. Male Sprague-Dawley rats were randomly assigned to five groups. In the first experiment, a full-thickness thermal injury or control procedure, covering 30% of the TBSA, was inflicted on three groups designated as the thermal injury, EGCG, and sham control groups...
February 6, 2017: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/28197358/biocomplexity-and-fractality-in-the-search-of-biomarkers-of-aging-and-pathology-focus-on-mitochondrial-dna-and-alzheimer-s-disease
#13
Annamaria Zaia, Pierluigi Maponi, Giuseppina Di Stefano, Tiziana Casoli
Alzheimer's disease (AD) represents one major health concern for our growing elderly population. It accounts for increasing impairment of cognitive capacity followed by loss of executive function in late stage. AD pathogenesis is multifaceted and difficult to pinpoint, and understanding AD etiology will be critical to effectively diagnose and treat the disease. An interesting hypothesis concerning AD development postulates a cause-effect relationship between accumulation of mitochondrial DNA (mtDNA) mutations and neurodegenerative changes associated with this pathology...
February 2017: Aging and Disease
https://www.readbyqxmd.com/read/28196517/simultaneous-detection-of-bovine-and-porcine-dna-in-pharmaceutical-gelatin-capsules-by-duplex-pcr-assay-for-halal-authentication
#14
Jafar Nikzad, Soraya Shahhosseini, Maryam Tabarzad, Nastaran Nafissi-Varcheh, Maryam Torshabi
BACKGROUND: In the pharmaceutical industry, hard- and soft-shelled capsules are typically made from gelatin, commonly derived from bovine and porcine sources. To ensure that pharmaceutical products comply with halal regulations in Muslim countries (no porcine products allowed), development of a valid, reliable, quick, and most importantly, cost-effective tests are of utmost importance. METHODS: We developed a species-specific duplex polymerase chain reaction (PCR) assay targeting 149 bp porcine and 271 bp bovine mitochondrial DNA (mtDNA) to simultaneously detect both porcine and bovine DNA (in one reaction at the same time) in gelatin...
February 14, 2017: Daru: Journal of Faculty of Pharmacy, Tehran University of Medical Sciences
https://www.readbyqxmd.com/read/28195532/horizontal-transfer-of-whole-mitochondria-restores-tumorigenic-potential-in-mitochondrial-dna-deficient-cancer-cells
#15
Lan-Feng Dong, Jaromira Kovarova, Martina Bajzikova, Ayenachew Bezawork-Geleta, David Svec, Berwini Endaya, Karishma Sachaphibulkij, Ana R Coelho, Natasa Sebkova, Anna Ruzickova, An S Tan, Katarina Kluckova, Kristyna Judasova, Katerina Zamecnikova, Zuzana Rychtarcikova, Vinod Gopalan, Margaryta Sobol, Bing Yan, Bijay Pattnaik, Naveen Bhatraju, Jaroslav Truksa, Pavel Stopka, Pavel Hozak, Alfred Lam, Radislav Sedlacek, Paulo J Oliveira, Mikael Kubista, Anurag Agrawal, Katerina Dvorakova-Hortova, Jakub Rohlena, Michael V Berridge, Jiri Neuzil
Recently we showed that generation of tumours in syngeneic mice by cells devoid of mitochondrial (mt) DNA (ρ(0) cells) is linked to acquisition of the host mtDNA. However, the mechanism of mtDNA movement between cells remains unresolved. To determine whether transfer of mtDNA involves whole mitochondria, we injected B16ρ(0) mouse melanoma cells into syngeneic C57BL/6N(su9-DsRed2) mice that express red fluorescent protein in their mitochondria. We document that mtDNA is acquired by transfer of whole mitochondria from the host animal, leading to normalisation of mitochondrial respiration...
February 14, 2017: ELife
https://www.readbyqxmd.com/read/28195213/spatial-assessment-of-wolf-dog-hybridization-in-a-single-breeding-period
#16
C Pacheco, J V López-Bao, E J García, F J Lema, L Llaneza, V Palacios, R Godinho
Understanding the dynamics of wolf-dog hybridization and delineating evidence-based conservation strategies requires information on the spatial extent of wolf-dog hybridization in real-time, which remains largely unknown. We collected 332 wolf-like scats over ca. 5,000km(2) in the NW Iberian Peninsula to evaluate wolf-dog hybridization at population level in a single breeding/pup-rearing season. Mitochondrial DNA (MtDNA) and 18 ancestry informative markers were used for species and individual identification, and to detect wolf-dog hybrids...
February 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28194828/mitochondrial-dysfunction-and-ovarian-aging
#17
Tianren Wang, Man Zhang, Zongliang Jiang, Emre Seli
Mitochondria are double-membrane-bound organelles that are responsible for the generation of most of the cell's energy. Mitochondrial dysfunction has been implicated in cellular senescence in general and ovarian aging in particular. Recent studies exploited this association by studying mitochondrial DNA (mtDNA) copy number as a potential biomarker of embryo viability and the use of mitochondrial nutrients and autologous mitochondrial transfer as a potential treatment for poor ovarian function and response.
February 13, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28193734/mitochondrial-depolarization-in-yeast-zygotes-inhibits-clonal-expansion-of-selfish-mtdna
#18
Iuliia E Karavaeva, Sergey A Golyshev, Ekaterina A Smirnova, Svyatoslav S Sokolov, Fedor F Severin, Dmitry A Knorre
Non-identical copies of mitochondrial DNA (mtDNA) compete with each other within a cell and the result depends on their replication rates. Using yeast Saccharomyces cerevisiae cells as a model, we studied the effects of mitochondrial inhibitors on the competition between wild type mtDNA and mutant selfish mtDNA in heteroplasmic zygotes. We found that decreasing mitochondrial transmembrane potential by adding uncouplers or valinomycin changes the competition outcomes in favor of the wild type mtDNA. This effect was significantly lower in the cells with disrupted mitochondria fission or repressed autophagy-related genes ATG8, ATG32 or ATG33 implying that heteroplasmic zygotes activate mitochondrial degradation in response to the depolarization...
February 13, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28193506/evaluation-of-genemarker-%C3%A2-hts-for-improved-alignment-of-mtdna-mps-data-haplotype-determination-and-heteroplasmy-assessment
#19
Mitchell M Holland, Erica D Pack, Jennifer A McElhoe
Existing software has not allowed for effective alignment of mitochondrial (mt) DNA sequence data generated using a massively parallel sequencing (MPS) approach, combined with the ability to perform a detailed assessment of the data. The regions of sequence that are typically difficult to align are homopolymeric stretches, isolated patterns of SNPs (single nucleotide polymorphisms), and INDELs (insertions/deletions). A custom software solution, GeneMarker(®) HTS, was developed and evaluated to address these limitations, and to provide a user-friendly interface for forensic practitioners and others interested in mtDNA analysis of MPS data...
February 6, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28192446/range-extension-for-the-common-dolphin-delphinus-sp-to-the-colombian-caribbean-with-taxonomic-implications-from-genetic-barcoding-and-phylogenetic-analyses
#20
Nohelia Farías-Curtidor, Dalia C Barragán-Barrera, Paula Alejandra Chávez-Carreño, Cristina Jiménez-Pinedo, Daniel M Palacios, Dalila Caicedo, Fernando Trujillo, Susana Caballero
The nearest known population of common dolphins (Delphinus sp.) to the Colombian Caribbean occurs in a fairly restricted range in eastern Venezuela. These dolphins have not been previously reported in the Colombian Caribbean, likely because of a lack of study of the local cetacean fauna. We collected cetacean observations in waters of the Guajira Department, northern Colombia (~11°N, 73°W) during two separate efforts: (a) a seismic vessel survey (December 2009-March 2010), and (b) three coastal surveys from small boats (May-July 2012, May 2013, and May 2014)...
2017: PloS One
keyword
keyword
34377
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"