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Melpomeni K Tangalidi, Ioannis L Oikonomidis, Dimitra Psalla, Serafim Papadimitriou, Maria Kritsepi-Konstantinou, Mathios E Mylonakis
A 5.5-year-old, intact male Rottweiler dog was admitted with a history of multifocal nodular tongue lesions which progressively deteriorated during the previous year. Physical examination revealed several reddish nodules with central depression on the surface of the tongue in an otherwise healthy dog. Clinicopathologic abnormalities included eosinophilia and hyperproteinemia. Lingual nodule cytopathology, histopathology, and immunohistochemistry revealed Leishmania spp. amastigotes and a severe granulomatous glossitis...
October 18, 2016: Veterinary Clinical Pathology
D Bravo-Barriga, R Parreira, C Maia, M O Afonso, J Blanco-Ciudad, F J Serrano, J E Pérez-Martín, L Gómez-Gordo, L Campino, D Reina, E Frontera
Leishmaniosis caused by Leishmania infantum is present in Mediterranean countries, with high prevalence in areas of the center and south of Spain. However, in some regions such as Extremadura (in southwest of Spain), data has not been updated since 1997. The aim of this work was (i) to provide information about the distribution of phlebotomine sand fly species in western of Spain (Extremadura region), (ii) to determine risk factors for the presence of sand fly vectors and (iii) to detect Leishmania DNA and identify blood meal sources in wild caught females...
October 5, 2016: Acta Tropica
Silvia Belinchón-Lorenzo, Juan Carlos Parejo, Virginia Iniesta, Javier Fernández-Cotrina, Rubén Muñoz-Madrid, Isabel Monroy, Victoria Baz, Adela Gómez-Luque, Francisco Javier Serrano-Aguilera, José Luis Barneto, Luis Carlos Gómez-Nieto
Nowadays, searching for alternative non-invasive methods for molecular diagnosis of canine visceral leishmaniosis is getting increasingly important. We previously described the presence of Leishmania kinetoplast DNA (kDNA) in canine hair; in this case we hypothesized whether foreign DNA might be present in cerumen of dogs with leishmaniosis, and be detected by Real time quantitative PCR (qPCR). A population of 38 dogs that lived in Leishmania endemic areas was divided in two groups: A (33 dogs with confirmed leishmaniosis by serological techniques) and B (5 healthy dogs)...
September 15, 2016: Veterinary Parasitology
Sara Montserrat-Sangrà, Lorena Alborch, Laura Ordeix, Laia Solano-Gallego
Innate immunity, in particular, the role of toll-like receptors (TLRs), has not been extensively studied in canine L. infantum infection. The main aim of this study was to determine the transcription of TLR2 and TLR4 in the blood of dogs with natural clinical leishmaniosis at the time of diagnosis and during treatment follow-up and subsequently correlate these findings with clinical, serological and parasitological data. Forty-six Leishmania-seropositive sick dogs with a high antibody level at the time of diagnosis were studied and compared with 34 healthy seronegative dogs...
September 15, 2016: Veterinary Parasitology
Marcus Tullius Scotti, Luciana Scotti, Hamilton Ishiki, Frederico Fávaro Ribeiro, Rayssa Marques Duarte da Cruz, Michelle Pedrosa de Oliveira, Francisco Jaime Bezerra Mendonça
Natural products are compounds extracted from plants, marine organisms, fungi or bacteria. Many researches for new drugs are based on these natural molecules, mainly by beneficial effects on health, health, efficacy, and therapeutic safety. Leishmaniosis, Chagas disease and African sleeping sickness are neglected diseases caused by the Leishmania and Trypanosoma ssp. parasites. These infections mainly affect population of developing countries; they have different symptoms, and may often lead to death. The therapeutic drugs available to treat these diseases are either obsolete, toxic, or have questionable efficacy, possibly through encountering resistance...
2016: Combinatorial Chemistry & High Throughput Screening
Silvana Ornelas Santos, Jonathan L M Fontes, Daniela F Laranjeira, José Vassallo, Stella Maria Barrouin-Melo, Washington L C Dos-Santos
Severe forms of zoonotic visceral leishmaniosis (ZVL) are associated with disruption of the spleen structure. However, the study of spleen histology requires splenectomy or necropsy. In this work, we present a minimally invasive cell-block technique for studying spleen tissue histology in dogs with ZVL. We examined 13 dogs with and seven dogs without Leishmania infantum infection. The dogs with Leishmania infection had a lower frequency of lymphoid follicles (2/13, Fisher's test, P<0.02) and a higher density of plasma cells (score 3, Fisher's test, P<0...
October 2016: Comparative Immunology, Microbiology and Infectious Diseases
Emanuele Brianti, Ettore Napoli, Gabriella Gaglio, Luigi Falsone, Salvatore Giannetto, Fabrizio Solari Basano, Roberto Nazzari, Maria Stefania Latrofa, Giada Annoscia, Viviana Domenica Tarallo, Dorothee Stanneck, Filipe Dantas-Torres, Domenico Otranto
This study investigated the efficacy of two collars for the treatment and prevention of flea infestations. Additionally the effect of these collars on the incidence of Leishmania infantum infection as compared with a group of vaccinated dogs was evaluated. A total of 224 young dogs from private animal shelters were enrolled in April/May into four groups: G1, 55 dogs treated with 10% imidacloprid + 4.5% flumethrin collar (Seresto, Bayer Animal Health); G2, 60 dogs treated with 4% deltamethrin collar (Scalibor protector band, MSD Animal Health); G3, 54 dogs vaccinated with CaniLeish (Virbac Animal Health); and G4, 55 dogs left non-treated as controls...
September 2016: PLoS Neglected Tropical Diseases
Ayşe Düzbeyaz, Nermin Şakru, Seray Töz
OBJECTIVE: In this study, we aimed to investigate the seroprevalence of canine leishmaniosis among dogs that live in the town center due to a lack of data on the prevalence of canine leishmaniasis (CanL) in Edirne. METHODS: In the present study, 37 dogs living in a municipal dog and cat shelter in Edirne were screened for leishmaniosis by the ındirect fluorescent antibody test. RESULTS: All samples were found to be seronegative. CONCLUSION: Our study is a preliminary study for Edirne...
June 2016: Türkiye Parazitolojii Dergisi
Pamela Rodrigues Reina Moreira, Douglas Augusto Franciscato, Sabrina Micelli Rossit, Danísio Prado Munari, Rosemeri de Oliveira Vasconcelos
The aim of this study was to evaluate apoptosis and parasite load in the liver and spleen of dogs with visceral leishmaniosis (VL), using immunohistochemistry. Liver and spleen samples from 71 dogs with VL were used. The parasite load in the spleen and liver showed significant difference between organs in infected group (P=0.0219). The density of the parasite load in the spleen (median=2.4) was higher than liver (median=0.8). Immunodetection of apoptotic cells was predominant in lymphocytes and differ between the infected and control group in spleen (P=0...
July 2016: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
Laia Solano-Gallego, Sara Montserrat-Sangrà, Laura Ordeix, Pamela Martínez-Orellana
No abstract text is available yet for this article.
2016: Parasites & Vectors
Ana Montoya, Lino Pérez de Quadros, Marta Mateo, Leticia Hernández, Rosa Gálvez, Gabriel Alcántara, Rocío Checa, María Ángeles Jiménez, Carmen Chicharro, Israel Cruz, Guadalupe Miró
Although dogs are the main reservoir for human Leishmania infantum infection, the disease has also been reported in other domestic and wild mammals. In 2011, a fatal case of naturally acquired leishmaniosis was described for the first time in a Bennett's wallaby (Macropus rufogriseus rufogriseus) kept in a wildlife park in Madrid (Spain). This study was designed to assess the infection status of twelve Bennett's wallabies in the same park one year after this incident. Phlebotomus perniciosus, the main vector of L...
June 2016: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
Marina Dal'Bó Pelegrini, Juliana Biar Pereira, Solange Dos Santos Costa, Myriam Janeth Salazar Terreros, Adriana Degrossoli, Selma Giorgio
OBJECTIVE: To evaluate whether hypoxia inducible factor (HIF-1α) targeting pharmacological drugs, echinomycin, resveratrol and CdCl2 which inhibit HIF-1α stimulation, and mimosine, which enhances the stability of HIF-1α present antileishmanial properties. METHODS: The leishmanicidal effect of drugs was evaluated in mouse macrophages and Balb/c mouse model for cutaneous leishmaniosis. RESULTS: Resveratrol and CdCl2 reduced the parasite load [IC50, (27...
July 2016: Asian Pacific Journal of Tropical Medicine
L Jaton, T Pillonel, K Jaton, E Dory, G Prod'hom, D S Blanc, F Tissot, P Bodenmann, G Greub
Since late 2014, multiple cases of abscesses and boils due to methicillin-susceptible Staphylococcus aureus (MSSA) expressing the Panton-Valentine leucocidin (PVL) were observed in Eritrean asylum seekers in Lausanne, Switzerland. Strains isolated from infected Eritrean and non-Eritrean patients were compared by whole genome sequencing to determine whether these numerous cases result from an outbreak. The genome of S. aureus PVL-producing strains were sequenced and compared. Clinical and epidemiological characteristics of patients infected by PVL-producing strains were investigated...
August 2016: Clinical Microbiology and Infection
Laia Solano-Gallego, Sara Montserrrat-Sangrà, Laura Ordeix, Pamela Martínez-Orellana
BACKGROUND: There is limited information available on cytokine profiles in dogs with different degrees of disease severity due to natural infection of Leishmania infantum. The aim of this study was to investigate L. infantum-specific IFN-γ and IL-10 production in blood from dogs with leishmaniosis at diagnosis and correlate these findings with disease severity, humoral immune response and blood parasitemia. METHODS: Sixty dogs were diagnosed based on physical examination, routine laboratory tests, L...
2016: Parasites & Vectors
Torsten J Naucke, Silke Amelung, Susanne Lorentz
BACKGROUND: Canine leishmaniosis (CanL) is an important zoonosis caused by Leishmania (L.) infantum. Transmission of L. infantum to dogs (and humans) is mainly through the bite of infected sandflies, but the parasite can also be transmitted vertically, venereally and through blood transfusions of infected donors. Additionally, the direct dog-to-dog transmission through bites or wounds is suspected. RESULTS: In December 2015, a female eight-year-old Jack-Russell-Terrier was tested positive for CanL in Germany (ELISA 74, IFAT 1:4...
2016: Parasites & Vectors
Gad Baneth, Yaarit Nachum-Biala, Maytal Shabat Simon, Ori Brenner, Sarit Gaier, Alicia Rojas, Daniel Yasur-Landau
BACKGROUND: Leishmania major is a main cause of cutaneous leishmaniasis in humans in an area that stretches from India through Central Asia, the Middle East, to North and West Africa. In Israel, it is a common infection of humans with rodents as the reservoir hosts and Phlebotomus papatasi as its sand fly vector. FINDINGS: A 6 months old spayed female mixed breed dog was referred to the Hebrew University Veterinary Teaching Hospital with a large ulcerative dermal lesion on the muzzle, and lesions in the foot pads and left hind leg...
2016: Parasites & Vectors
André Pereira, Ângela Martins, Hugo Brancal, Hugo Vilhena, Pedro Silva, Paulo Pimenta, Duarte Diz-Lopes, Nuno Neves, Mónica Coimbra, Ana Catarina Alves, Luís Cardoso, Carla Maia
BACKGROUND: Parasitic diseases of companion animals comprise a group of globally distributed and rapidly spreading illnesses that are caused by a wide range of arthropods, helminths and protozoa. In addition to their veterinary importance, many of these parasites can also affect the human population, due to their zoonotic potential. The aim of the present work was to evaluate the knowledge of Portuguese pet owners regarding the zoonotic potential of parasites that dogs and cats can harbour, most common drugs, frequency of use and reasons for endo- and ectoparasite control...
2016: Parasites & Vectors
Laia Solano-Gallego, Laura Di Filippo, Laura Ordeix, Marta Planellas, Xavier Roura, Laura Altet, Pamela Martínez-Orellana, Sara Montserrat
BACKGROUND: Leishmania infantum-specific antibodies are used extensively for the diagnosis and monitoring of treatment in canine leishmaniosis. Different views have been described for the measurement of L. infantum antibody levels for the monitoring of anti-leishmanial treatment. In addition, molecular techniques using blood are frequently employed in the clinical setting. However, there are not enough studies to prove the usefulness of PCR in diagnosis, treatment monitoring and in assessing the prognosis of the disease...
2016: Parasites & Vectors
Carla Maia, Laura Altet, Lorena Serrano, José Manuel Cristóvão, Maria Dolores Tabar, Olga Francino, Luís Cardoso, Lenea Campino, Xavier Roura
BACKGROUND: Leishmaniosis caused by the protozoan Leishmania infantum and dirofilariosis caused by the nematodes Dirofilaria immitis or Dirofilaria repens are vector-borne zoonoses widely present in the Mediterranean basin. In addition, some studies reported that the endosymbiont Wolbachia spp. play a role in the biology and pathogenesis of filarial parasites. The aim of this work was to evaluate the frequency of mono- and co-infections by L. infantum, filariae and Wolbachia spp. and their association with clinical signs in dogs from the south of Portugal...
2016: Parasites & Vectors
D Matralis, E Papadogiannakis, V Kontos, E Papadopoulos, E Ktenas, A Koutinas
Canine leishmaniosis (CanL) is a systemic zoonotic disease the clinical manifestations of which can range from self-healing cutaneous lesions to disseminated visceral disease. Effective activation of cellular immunity is the cornerstone of resistance against Leishmania infantum in infected dogs. The aim of this cross-sectional, controlled study was the intracellular detection of interleukin 4 (IL-4) and interferon-γ (IFN-γ) in CD4+ and CD8+ lymphocytes in the peripheral blood of 40 dogs naturally infected with L...
August 2016: Parasite Immunology
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