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Bacillus amyloliquefaciens

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https://www.readbyqxmd.com/read/29134097/the-optimization-of-fermentation-conditions-for%C3%A2-producing-cellulase-of-bacillus-amyloliquefaciens-and-its-application-to-goose%C3%A2-feed
#1
Miao Ye, Linghong Sun, Ru Yang, Zaigui Wang, KeZong Qi
The proper culture conditions for producing cellulase of Bacillus amyloliquefaciens S1, isolated from the cecum of goose was optimized by single-factor experiment combined with orthogonal test. The properties of the cellulase were investigated by DNS method. The appropriate doses of B. amyloliquefaciens S1 were obtained by adding them to goose feed. It indicated that the suitable culture conditions of producing cellulase were the culture temperature of 37°C, the initial pH of 7.0, the incubation time of 72 h and the loaded liquid volume of 75 ml per 250 ml...
October 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/29124466/antifungal-activity-of-avocado-rhizobacteria-against-fusarium-euwallaceae-and-graphium-spp-associated-with-euwallacea-spp-nr-fornicatus-and-phytophthora-cinnamomi
#2
Edgar Guevara-Avendaño, Joseph D Carrillo, Cedric Ndinga-Muniania, Kevin Moreno, Alfonso Méndez-Bravo, José A Guerrero-Analco, Akif Eskalen, Frédérique Reverchon
Plant rhizobacteria have been successfully used as biocontrol agents against fungal phytopathogens. However, their potential to control two important avocado diseases, namely Fusarium dieback (FD) and Phytophthora root rot (PRR), has been poorly studied. FD is an emerging disease triggered by fungi associated with two ambrosia beetle species (Euwallacea fornicatus species complex), while PRR is caused by Phytophthora cinnamomi, a soil-borne oomycete. In the present work, the antifungal activity of bacteria isolated from avocado rhizosphere was tested in dual culture assays against Fusarium euwallaceae, Graphium euwallaceae and Graphium sp...
November 9, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29121702/preventive-effects-of-a-probiotic-mixture-in-an-ovalbumin-induced-food-allergy-model
#3
Hee-Soon Shin, Ji-Eun Eom, Dong-Uk Shin, Sung-Hum Yeon, Seong-Il Lim, So-Young Lee
Although there has been a steady increase in the prevalence of food allergies worldwide in recent decades, no effective therapeutic strategies have been developed. Modulation of the gut microbiota composition and/or function through probiotics has been highlighted as a promising target for protection against food allergies. In this study, we aimed to investigate the allergy-reducing effects of a probiotic mixture (P5: Lactococcus lactis KF140, Pediococcus pentosaceus KF159, Lactobacillus pentosus KF340, Lactobacillus paracasei 698, and Bacillus amyloliquefaciens 26N) in mice with ovalbumin (OVA)-induced food allergy...
November 9, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29111704/new-method-to-produce-kokumi-seasoning-from-protein-hydrolysates-using-bacterial-enzymes
#4
Hideyuki Suzuki, Yuko Nakafuji, Tomoki Tamura
In the present study, we demonstrate a novel use for a commercially available glutaminase that can be used as a γ-glutamyltranspeptidase in kokumi seasoning production. Soy protein and gluten were hydrolyzed using a protease isolated from Bacillus licheniformis. The resulting protein hydrolysates were γ-glutamylated with a γ-glutamyltranspeptidase, which is sold as a glutaminase from Bacillus amyloliquefaciens, to produce kokumi seasonings. For γ-glutamylation of soy protein hydrolysate, glutamine was added to the reaction mixture...
November 17, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29101733/bioprocessing-of-biodiesel-industry-effluent-by-immobilized-bacteria-to-produce-value-added-products
#5
Jyotsana Prakash, Rahul Kumar Gupta, Priyanka Xx, Vipin Chandra Kalia
Biodiesel industrial effluent rich in crude glycerol (CG) was processed to produce value-added product. Under continuous culture system, Bacillus amyloliquefaciens strain CD16 immobilized within its biofilm, produced 3.2 L H2/day/L feed, over a period of 60 days at a hydraulic retention time of 2 days. The effective H2 yield by B. amyloliquefaciens strain CD16 was 165 L/L CG. This H2 yield was 1.18-fold higher than that observed with non-biofilm forming Bacillus thuringiensis strain EGU45. Bioprocessing of the effluent released after this stage, by recycling it up to 25% did not have any adverse effect on H2 production by strain EGU45; however, a 25% reduction in yield was recorded with strain CD16...
November 4, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29101598/determination-and-optimization-of-a-strong-promoter-element-from-bacillus-amyloliquefaciens-by-using-a-promoter-probe-vector
#6
Yuling Liao, Bin Wang, Yanrui Ye, Li Pan
OBJECTIVE: To construct a promoter probe vector, pBE-bgaB, to screen strong promoters from Bacillus amyloliquefaciens. RESULTS: 266 colonies containing active promoter elements from the genomic DNA of B. amyloliquefaciens were identified. Among these, promoter P41 exhibited the strongest β-Gal activity in Escherichia coli and B. amyloliquefaciens. Sequence analysis showed that promoter P41 contained P ykuN , a ykuN gene encoding flavodoxin. Optimization of the ribosome-binding site from P41 to P382 improved β-Gal activity by ~ 200%...
November 3, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/29090851/bacillus-amyloliquefaciens-fzb42-repressed-plant-mir846-to-induce-systemic-resistance-via-jasmonic-acid-dependent-signaling-pathway
#7
Shanshan Xie, Haiyang Jiang, Ting Ding, Qianqian Xu, Wenbo Chai, Beijiu Cheng
Bacillus amyloliquefaciens FZB42 is a kind of plant-growth-promoting rhizobacteria (PGPR) that activates induced systemic resistance (ISR) in Arabidopsis. Blocking in cyclic lipopeptides and 2,3-butanediol synthesis of FZB42, which were proved to be involved in priming ISR, resulted in abolishment of plant defense responses. To further clarify the ISR activated by PGPR at miRNA level, sRNA libraries from Arabidopsis leaves after root-irrigated with FZB42, FZB42ΔsfpΔalsS and control were constructed and sequenced...
November 1, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29077888/effects-of-direct-fed-microorganisms-and-enzyme-blend-co-administration-on-intestinal-bacteria-in-broilers-fed-diets-with-or-without-antibiotics
#8
T E Askelson, C A Flores, S L Dunn-Horrocks, Y Dersjant-Li, K Gibbs, A Awati, J T Lee, T Duong
Direct-fed microorganisms (DFM) and exogenous enzymes have been demonstrated to improve growth performance in poultry and are potentially important alternatives to antibiotic growth promoters (AGP). We investigated the administration of a feed additive composed of a DFM product containing spores of 3 Bacillus amyloliquefaciens strains and an enzyme blend of endo-xylanase, α-amylase, and serine-protease in diets with or without sub-therapeutic antibiotics in broiler chickens over a 42-d growth period. Evaluation of growth performance determined feed efficiency of broiler chickens which were administered the feed additive was comparable to those fed a diet containing AGPs...
October 25, 2017: Poultry Science
https://www.readbyqxmd.com/read/29077011/identification-for-the-first-time-of-cyclo-d-pro-l-leu-produced-by-bacillus-amyloliquefaciens-y1-as-a-nematocide-for-control-of-meloidogyne-incognita
#9
Qaiser Jamal, Jeong-Yong Cho, Jae-Hak Moon, Shahzad Munir, Muhammad Anees, Kil Yong Kim
The aim of the current study was to describe the role and mechanism of Bacillus amyloliquefaciens Y1 against the root-knot nematode, Meloidogyne incognita, under in vitro and in vivo conditions. Initially, the exposure of the bacterial culture supernatant and crude extract of Y1 to M. incognita significantly inhibited the hatching of eggs and caused the mortality of second-stage juveniles (J2), with these inhibitory effects depending on the length of incubation time and concentration of the treatment. The dipeptide cyclo(d-Pro-l-Leu) was identified in B...
October 27, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29071169/draft-genome-sequence-of-a-thermostable-alkaliphilic-%C3%AE-amylase-and-protease-producing-bacillus-amyloliquefaciens-strain-kcp2
#10
Vimalkumar S Prajapati, Sanket Ray, Jitendra Narayan, Chaitanya C Joshi, Kamlesh C Patel, Ujjval B Trivedi, R M Patel
Bacillus amyloliquefaciens strain KCP2 was isolated from municipal food waste samples collected in Vallabh Vidyanagar, Gujarat, India. Strain KCP2 is noteworthy due to its ability to produce a thermostable, alkaliphilic α-amylase and a protease. These enzymes have importance in several industrial processes including bread making, brewing, starch processing, pharmacy, and textile industries. Whole genome sequencing of strain KCP2 showed that the estimated genome size was 3.9 Mb, the G + C content was 46%, and it coded for 4113 genes...
December 2017: 3 Biotech
https://www.readbyqxmd.com/read/29063401/biofilm-formation-is-determinant-in-tomato-rhizosphere-colonization-by-bacillus-velezensis-fzb42
#11
Ameen Al-Ali, Jovana Deravel, François Krier, Max Béchet, Marc Ongena, Philippe Jacques
In this work, the behavior in tomato rhizosphere of Bacillus velezensis FZB42 was analyzed taking into account the surfactin production, the use of tomato roots exudate as substrates, and the biofilm formation. B. velezensis FZB42 and B. amyloliquefaciens S499 have a similar capability to colonize tomato rhizosphere. Little difference in this colonization was observed with surfactin non producing B. velezensis FZB42 mutant strains. B. velezensis is able to grow in the presence of root exudate and used preferentially sucrose, maltose, glutamic, and malic acids as carbon sources...
October 23, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29045133/the-product-of-yersinia-pseudotuberculosis-mcc-operon-is-a-peptide-cytidine-antibiotic-activated-inside-producing-cells-by-the-tldd-e-protease
#12
Darya Tsibulskaya, Olga Mokina, Alexey Kulikovsky, Julia Piskunova, Konstantin Severinov, Marina Serebryakova, Svetlana Dubiley
Microcin C is a heptapeptide-adenylate antibiotic produced by some strains of Escherichia coli. Its peptide part is responsible for facilitated transport inside sensitive cells where it is proteolyzed with release of a toxic warhead-a nonhydrolyzable aspartamidyl-adenylate, which inhibits aspartyl-tRNA synthetase. Recently, a microcin C homologue from Bacillus amyloliquefaciens containing a longer peptide part modified with carboxymethyl-cytosine instead of adenosine was described, but no biological activity of this compound was revealed...
October 31, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29044981/use-of-biocontrol-agents-and-botanicals-in-integrated-management-of-botrytis-cinerea-in-table-grape-vineyards
#13
Caterina Rotolo, Rita M De Miccolis Angelini, Crescenza Dongiovanni, Stefania Pollastro, Giulio Fumarola, Michele Di Carolo, Donato Perrelli, Patrizia Natale, Francesco Faretra
BACKGROUND: The increasing interest on biological control agents (BCAs) and botanicals (BOTs) is due to the increasing awareness on environmental and human health risks associated to the usage of synthetic plant protection products. The BCAs Bacillus subtilis strain QST713, Bacillus amyloliquefaciens strain D747 and Aureobasidium pullulans strains DSM14940 and DSM14941, and the BOTs Melaleuca alternifolia and terpenic extracts are proposed for the control of grey mould in vineyard. This study was aimed at evaluating their effectiveness in integrated crop management strategies and their outcomes in terms of management of fungicide resistance and residues...
October 17, 2017: Pest Management Science
https://www.readbyqxmd.com/read/29018313/characterization-of-bacillus-amyloliquefaciens-da12-showing-potent-antifungal-activity-against-mycotoxigenic-fusarium-species
#14
Theresa Lee, Dami Park, Kihyun Kim, Seong Mi Lim, Nan Hee Yu, Sosoo Kim, Hwang-Yong Kim, Kyu Seok Jung, Ja Yeong Jang, Jong-Chul Park, Hyeonheui Ham, Soohyung Lee, Sung Kee Hong, Jin-Cheol Kim
In an attempt to develop a biological control agent against mycotoxigenic Fusarium species, we isolated Bacillus amyloliquefaciens strain DA12 from soil and explored its antimicrobial activities. DA12 was active against the growth of mycotoxigenic F. asiaticum, F. graminearum, F. proliferatum, and F. verticillioides both in vitro and in planta (maize). Further screening using dual culture extended the activity range of strain DA12 against other fungal pathogens including Botrytis cinerea, Colletotrichum coccodes, Endothia parasitica, Fusarium oxysporum, Raffaelea quercus-mongolicae, and Rhizoctonia solani...
October 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/28990361/characterization-of-garlic-endophytes-isolated-from-the-black-garlic-processing
#15
Zhichang Qiu, Xiaoming Lu, Ningyang Li, Mingjie Zhang, Xuguang Qiao
The objectives of this study were to isolate and identify garlic endophytes, and explore the characteristics of dominant strains. Garlic endophytes were studied through phenotypical characterization and comparative sequence analysis of 16S rDNA based on culture-dependent approaches. Representative strains inferred from 16S rDNA sequencing were selected for further identification by gyrA and rpoB gene loci and phylogenetic analysis based on concateneted house-keeping sequences. Seven kinds of Bacillus were found from garlic and black garlic, respectively...
October 9, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28978203/silver-engineered-nanomaterials-and-ions-elicit-species-specific-o2-consumption-responses-in-plant-growth-promoting-rhizobacteria
#16
Ricky W Lewis, Jason Unrine, Paul M Bertsch, David H McNear
Metal containing engineered nanomaterials (ENMs) are now commonly used in various industrial and commercial applications. Many of these materials can be transformed during waste water treatment and ultimately enter terrestrial ecosystems via agriculturally applied biosolids. It is unclear how agriculturally important soil microbes will be affected by exposure to environmentally relevant, sublethal concentrations of ENMs and their transformation products (i.e., ions, aggregates, etc.). A method was developed, which puts O2 consumption responses in terms of viability, and tested by examining the toxic effects of Ag(+), Zn(2+), and Ni(2+) ions on the plant growth promoting rhizobacterium (PGPR) Bacillus amyloliquefaciens GB03...
October 4, 2017: Biointerphases
https://www.readbyqxmd.com/read/28961818/insights-into-the-molecular-basis-of-biocontrol-of-brassica-pathogens-by-bacillus-amyloliquefaciens-ucmb5113-lipopeptides
#17
Shashidar Asari, Marc Ongena, Delphine Debois, Edwin De Pauw, Kunling Chen, Sarosh Bejai, Johan Meijer
Background and Aims: Certain micro-organisms can improve plant protection against pathogens. The protective effect may be direct, e.g. due to antibiotic compounds, or indirect, by priming of plant defence as induced systemic resistance (ISR). The plant growth-promoting rhizobacterium Bacillus amyloliquefaciens UCMB5113 shows potential for disease management of oilseed rape. To investigate the mode of action of this protection, especially in relation to jasmonic acid-dependent ISR, Bacillus UCMB5113 was tested with Arabidopsis thaliana mutants and several important fungal pathogens of Brassica species...
October 17, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28955633/effect-of-differential-processing-of-the-native-and-recombinant-%C3%AE-amylase-from-bacillus-amyloliquefaciens-jjc33m-on-specificity-and-enzyme-properties
#18
Juan José Montor-Antonio, Sarahi Hernández-Heredia, Ángela Ávila-Fernández, Clarita Olvera, Bernardo Sachman-Ruiz, Sandra Del Moral
AmyJ33, an α-amylase isolated from Bacillus amyloliquefaciens JJC33M, has been characterized as a non-metalloenzyme that hydrolyzes raw starch. In this work, the gene that codifies for AmyJ33 was isolated and cloned. The recombinant α-amylase (AmyJ33r) produced had a molecular weight of 72 kDa, 25 kDa higher than the native α-amylase (AmyJ33). Our results suggest that the C-terminal was processed in a different way in the native and the recombinant enzyme causing the difference observed in the molecular weight...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28936838/-isolation-and-identification-of-rhizobacteria-rs-3-from-panax-ginseng-and-evaluation-of-its-antagonistic-activity
#19
Tie-Lin Wang, Dong-Yue Zhao, Mei-Lan Chen, Zhong-Hua Ma, Lan-Ping Guo
A rhizobacteria strain named RS-3 exhibited inhibitory activity against all five Panax ginseng pathogens was isolated from the root of P. ginseng. This strain was identified as Bacillus amyloliquefaciens based on its morphological character and 16S rDNA sequence. Antagonistic activity experiments indicated that the strain could strongly suppress Botrytis cinerea Pers with an inhibitory rate of 54.4%, suggesting the potentialities of biocontrol agent against diseases that frequently happen on ginseng.
December 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28925953/transesterification-synthesis-of-chloramphenicol-esters-with-the-lipase-from-bacillus-amyloliquefaciens
#20
Fengying Dong, Lingmeng Li, Lin Lin, Dannong He, Jingwen Chen, Wei Wei, Dongzhi Wei
This work presents a synthetic route to produce chloramphenicol esters by taking advantage the high enantio- and regio-selectivity of lipases. A series of chloramphenicol esters were synthesized using chloramphenicol, acyl donors of different carbon chain length and lipase LipBA (lipase cloned from Bacillus amyloliquefaciens). Among acyl donors with different carbon chain lengths, vinyl propionate was found to be the best. The influences of different organic solvents, reaction temperature, reaction time, enzyme loading and water content on the synthesis of the chloramphenicol esters were studied...
September 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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