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Bacillus amyloliquefaciens

Su Jin Kang, Ji Yeon Seo, Kye Man Cho, Chang Kwon Lee, Jeong Hwan Kim, Jong-Sang Kim
A new type of doenjang was manufactured by mixing soaked soybean, koji (Rhizopus oryzae), cheonggukjang (Bacillus amyloliquefaciens MJ1-4 and B. amyloliquefaciens EMD17), and Pichia farinosa SY80 as a yeast, salt, and water, followed by fermentation with koji that was made by fermenting whole wheat with R. oryzae. The mixed culture doenjang was designed to have a more palatable flavor and stronger biological activities than the conventional product. The extract of mixed culture doenjang showed higher antioxidant activity than the commercial doenjang as evaluated by the ferric reducing antioxidant power assay although it was not significantly different from the commercial product in 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities...
September 2016: Preventive Nutrition and Food Science
Ana Santana, Armindo Melo, Tânia Tavares, Isabel M P L V O Ferreira
The increase of hen egg consumption demands profitable applications for eggshells, including their membranes, in order to minimize environmental and public health problems that could result from their accumulation. This work presents an innovative application for eggshell membranes to obtain an added-value food ingredient that combines maximized ACE-inhibitory and antioxidant activities. Firstly, the use of acetic acid 5% (v/v); and 3-mercaptopropionic acid 1.25 M enabled 63% recovery of eggshell membrane proteins...
October 17, 2016: Food & Function
Chaoyang Zhou, Lingling Shi, Bin Ye, Haichao Feng, Ji Zhang, Ruifu Zhang, Xin Yan
Aside from applications in the production of commercial enzymes and metabolites, Bacillus amyloliquefaciens is also an important group of plant growth-promoting rhizobacteria that supports plant growth and suppresses phytopathogens. A host-genotype-independent counter-selectable marker would enable rapid genetic manipulation and metabolic engineering, accelerating the study of B. amyloliquefaciens and its development as both a microbial cell factory and plant growth-promoting rhizobacteria. Here, a host-genotype-independent counter-selectable marker pheS (*) was constructed through a point mutation of the gene pheS, which encodes the α-subunit of phenylalanyl-tRNA synthetase in Bacillus subtilis strain 168...
October 11, 2016: Applied Microbiology and Biotechnology
Young Soo Kim, Kotnala Balaraju, Yongho Jeon
AIMS: The effect of Bacillus amyloliquefaciens AK-0 (AK-0) on ginseng root-rot disease caused by Cylindrocarpon destructans was investigated. METHODS AND RESULTS: From 190 ginseng rhizoshpere bacteria, AK-0 was selected for further analysis; its morphological characteristics were investigated by microscopy. AK-0 was identified as B. amyloliquefaciens using the Biolog system, 16S rRNA gene sequence analysis, and examination of morphological and biochemical characteristics...
October 11, 2016: Journal of Applied Microbiology
Qi Zhao, Yong-Na Wu, Qin Fan, Qing-Qing Han, Paul W Pare, Rui- Xu, Yin-Quan Wang, Suo-Min Wang, Jin-Lin Zhang
Codonopsis pilosula (Franch.) Nannf. is a traditional Chinese herbal medicinal plant and a low-cost succedaneum for Panax ginseng and contains all various of bioactivity components. In this work, we firstly evaluated the effects of the inoculation of the plant growth-promoting rhizobacteria Bacillus amyloliquefaciens strain GB03 on growth and metabolites accumulation of C. pilosula. The results demonstrated that application of B. amyloliquefaciens GB03 significantly improved the growth of C. pilosula compared to DH5α, LB medium and water treatment, respectively...
October 10, 2016: Journal of Agricultural and Food Chemistry
Rebecca C Gregory, Glyn R Hemsworth, Johan P Turkenburg, Samuel J Hart, Paul H Walton, Gideon J Davies
The enzymatic deconstruction of recalcitrant polysaccharide biomass is central to the conversion of these substrates for societal benefit, such as in biofuels. Traditional models for enzyme-catalysed polysaccharide degradation involved the synergistic action of endo-, exo- and processive glycoside hydrolases working in concert to hydrolyse the substrate. More recently this model has been succeeded by one featuring a newly discovered class of mononuclear copper enzymes: lytic polysaccharide monooxygenases (LPMOs; classified as Auxiliary Activity (AA) enzymes in the CAZy classification)...
September 29, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
Kajal Chakraborty, Bini Thilakan, Vamshi Krishna Raola, Minju Joy
Heterotrophic Bacillus amyloliquefaciens associated with edible red seaweed, Laurenciae papillosa was used to isolate antibacterial polyketide compounds. Antibacterial activity studies integrated with the outcome obtained by polyketide synthetase (pks) coding genes established that seaweed-affiliated bacterial flora had a wide-ranging antibacterial activities and potential natural product diversity, which proved that the bacterium is valuable reservoir of novel bioactive metabolites. Bioactivity-guided isolation of 3-(octahydro-9-isopropyl-2H-benzo[h]chromen-4-yl)-2-methylpropyl benzoate and methyl 8-(2-(benzoyloxy)-ethyl)-hexahydro-4-((E)-pent-2-enyl)-2H-chromene-6-carboxylate of polyketide origin, with activity against human opportunistic food pathogenic microbes, have been isolated from the ethyl acetate extract of B...
March 1, 2017: Food Chemistry
Jian Jiao, Yaner Ma, Sha Chen, Chonghuai Liu, Yuyang Song, Yi Qin, Chunlong Yuan, Yanlin Liu
Endophytes form symbiotic relationships with plants and constitute an important source of phytohormones and bioactive secondary metabolites for their hosts. To date, most studies of endophytes have focused on the influence of these microorganisms on plant growth and physiology and their role in plant defenses against biotic and abiotic stressors; however, to the best of our knowledge, the ability of endophytes to produce melatonin has not been reported. In the present study, we isolated and identified root-dwelling bacteria from three grapevine varieties and found that, when cultured under laboratory conditions, some of the bacteria strains secreted melatonin and tryptophan-ethyl ester...
2016: Frontiers in Plant Science
Shumei Zhang, Wei Jiang, Jing Li, Liqiang Meng, Xu Cao, Jihua Hu, Yushuai Liu, Jingyu Chen, Changqing Sha
Bacillus amyloliquefaciens TF28 is a biocontrol endophytic bacterium that is capable of inhibition of a broad range of plant pathogenic fungi. The strain has the potential to be developed into a biocontrol agent for use in agriculture. Here we report the whole-genome shotgun sequence of the strain. The genome size of B. amyloliquefaciens TF28 is 3,987,635 bp which consists of 3754 protein-coding genes, 65 tandem repeat sequences, 47 minisatellite DNA, 2 microsatellite DNA, 63 tRNA, 7rRNA, 6 sRNA, 3 prophage and CRISPR domains...
2016: Standards in Genomic Sciences
Xunchao Cai, Xingxing Kang, Huan Xi, Changhong Liu, Yarong Xue
Bacillus velezensis is a heterotypic synonym of B. methylotrophicus, B. amyloliquefaciens subsp. plantarum, and Bacillus oryzicola, and has been used to control plant fungal diseases. In order to fully understand the genetic basis of antimicrobial capacities, we did a complete genome sequencing of the endophytic B. velezensis strain CC09. Genes tightly associated with biocontrol ability, including nonribosomal peptide synthetases, polyketide synthetases, iron acquisition, colonization, and volatile organic compound synthesis were identified in the genome...
September 29, 2016: Genome Announcements
Qingshu Liu, Qiyao Shen, Xiaoying Bian, Hanna Chen, Jun Fu, Hailong Wang, Ping Lei, Zhaohui Guo, Wu Chen, Dingjun Li, Youming Zhang
Heterologous expression of biosynthetic pathways is an important way to research and discover microbial natural products. Bacillus subtilis is a suitable host for the heterologous production of natural products from bacilli and related Firmicutes. Existing technologies for heterologous expression of large biosynthetic gene clusters in B. subtilis are complicated. Herein, we present a simple and rapid strategy for direct cloning based heterologous expression of biosynthetic pathways in B. subtilis via Red/ET recombineering, using a 5...
September 30, 2016: Scientific Reports
Giulia Molinatto, Gerardo Puopolo, Paolo Sonego, Marco Moretto, Kristof Engelen, Carlo Viti, Marc Ongena, Ilaria Pertot
Bacillus amyloliquefaciens subsp. plantarum S499 is a plant beneficial rhizobacterium with a good antagonistic potential against phytopathogens through the release of active secondary metabolites. Moreover, it can induce systemic resistance in plants by producing considerable amounts of surfactins. The complete genome sequence of B. amyloliquefaciens subsp. plantarum S499 includes a circular chromosome of 3,927,922bp and a plasmid of 8,008bp. A remarkable abundance in genomic regions of putative horizontal origin emerged from the analysis...
November 20, 2016: Journal of Biotechnology
Febriyansyah Saputra, Ya-Li Shiu, Yo-Chia Chen, Asthervina Widyastami Puspitasari, Ridha Handriany Danata, Chun-Hung Liu, Shao-Yang Hu
Bacillus amyloliquefaciens has attracted attention as a probiotic in aquaculture due to its immunostimulatory activity against pathogenic infection. Xylanases are extensively used in animal feed to degrade plant ingredients, enhancing nutrient utilization and increasing the growth rate of various animals. In the present study, the effects of dietary supplementation with B. amyloliquefaciens and xylanase-expressing B. amyloliquefaciens R8 on the growth of Nile tilapia (Oreochromis niloticus) and immunity against Aeromonas hydrophila were evaluated...
September 23, 2016: Fish & Shellfish Immunology
Ponnusamy Manogaran Gopinath, Anandan Ranjani, Dharumadurai Dhanasekaran, Nooruddin Thajuddin, Govindaraju Archunan, Mohammad Abdulkader Akbarsha, Balázs Gulyás, Parasuraman Padmanabhan
The present study was aimed at evaluating the fluorescence property, sporicidal potency against Bacillus and Clostridium endospores, and surface disinfecting ability of biogenic nano silver. The nano silver was synthesized using an actinobacterial cell-filtrate. The fluorescence property as well as imaging facilitator potency of this nano silver was verified adopting spectrofluorometer along with fluorescent and confocal laser scanning microscope wherein strong emission and bright green fluorescence, respectively, on the entire spore surface was observed...
September 26, 2016: Scientific Reports
Elham Karimi, Naser Safaie, Masoud Shams-Baksh, Bagher Mahmoudi
The use of biocontrol strains recently has become a popular alternative to conventional chemical treatments. A set of bacteria isolated from sugar beet rhizosphere and from roots and shoots of apple and walnut were evaluated for their potential to control sugar beet seedling damping-off caused by R. solani AG-4 and AG2-2.The results of in vitro assays concluded that three isolates, SB6, SB14, SB15, obtained from rhizosphere of sugar beet and five isolates, AP2, AP4, AP6, AP7, AP8, obtained from shoots and roots of apple were the most effective antagonists that inhibited the mycelial growth of both R...
November 2016: Microbiological Research
Imen Zalila-Kolsi, Afif Ben Mahmoud, Hacina Ali, Sameh Sellami, Zina Nasfi, Slim Tounsi, Kaïs Jamoussi
Bacillus species are attractive due to their potential use in the biological control of fungal diseases. Bacillus amyloliquefaciens strain BLB369, Bacillus subtilis strain BLB277, and Paenibacillus polymyxa strain BLB267 were isolated and identified using biochemical and molecular (16S rDNA, gyrA, and rpoB) approaches. They could produce, respectively, (iturin and surfactin), (surfactin and fengycin), and (fusaricidin and polymyxin) exhibiting broad spectrum against several phytopathogenic fungi. In vivo examination of wheat seed germination, plant height, phenolic compounds, chlorophyll, and carotenoid contents proved the efficiency of the bacterial cells and the secreted antagonist activities to protect Tunisian durum wheat (Triticum turgidum L...
November 2016: Microbiological Research
Chu-Ning Huang, Chan-Pin Lin, Feng-Chia Hsieh, Sook-Kuan Lee, Kuan-Chen Cheng, Chi-Te Liu
Bacillus amyloliquefaciens strain WF02, isolated from soil collected at Wufeng Mountain, Taiwan, has siderophore-producing ability and in vitro antagonistic activity against bacterial wilt pathogen. To determine the impact of plant genotype on biocontrol effectiveness, we treated soil with this strain before infecting susceptible (L390) and moderately resistant (Micro-Tom) tomato cultivars with Ralstonia solanacearum strain Pss4. We also compared the efficacy of this strain with that of commercial Bacillus subtilis strain Y1336...
November 2016: World Journal of Microbiology & Biotechnology
Hedong Lu, Shiquan Qian, Umair Muhammad, Xingyi Jiang, Jinzhi Han, Zhaoxin Lu
AIMS: To explore the influence of carbohydrates on the production of fengycin by Bacillus amyloliquefaciens fmb-60. METHODS AND RESULTS: Fermentation and gene expression levels were examined following culture supplementation with fructose, arabinose and sorbitol. Compared with other carbohydrates, fructose most efficiently increased the concentration of the essential component amino acids for fengycin synthesis in the cell. The maximum production of fengycin was found to be 392...
September 9, 2016: Journal of Applied Microbiology
Benjamin J Schofield, Adam Skarshewski, Nancy Lachner, Diane Ouwerkerk, Athol V Klieve, Peter Dart, Philip Hugenholtz
Bacillus amyloliquefaciens H57 is a bacterium isolated from lucerne for its ability to prevent feed spoilage. Further interest developed when ruminants fed with H57-inoculated hay showed increased weight gain and nitrogen retention relative to controls, suggesting a probiotic effect. The near complete genome of H57 is ~3.96 Mb comprising 16 contigs. Within the genome there are 3,836 protein coding genes, an estimated sixteen rRNA genes and 69 tRNA genes. H57 has the potential to synthesise four different lipopeptides and four polyketide compounds, which are known antimicrobials...
2016: Standards in Genomic Sciences
Xunhang Li, Yanzhou Zhang, Zhiwen Wei, Zhengbing Guan, Yujie Cai, Xiangru Liao
The gummosis disease is caused by Botryosphaeria dothidea (Moug. ex. Fr) Ces. et de Not., and it is one of the most important diseases of stone fruits worldwide. The use of biocontrol as an alternative approach to synthetic chemical fungicides has aroused general concern about how to control plant diseases that are caused by phytopathogens. The aim of this study is to isolate Bacillus strains from raw honeys with the capacity to inhibit B. dothidea and to explore the mechanisms by which they could be used in the biocontrol of peach gummosis...
2016: PloS One
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