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Bacillus amyloliquefaciens

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https://www.readbyqxmd.com/read/28545259/identification-of-diacetonamine-from-soybean-curd-residue-as-a-sporulation-inducing-factor-toward-bacillus-spp
#1
Aki Ikeda, Dongyeop Kim, Yasuyuki Hashidoko
Under bioassay-guided investigation, a sporulation-inducing factor (SIF) toward Bacillus spp. was searched for in methanol (MeOH) extracts of soybean curd residues, and diacetonamine (1) was identified as the active compound. SIF was first isolated as a monoacetylated derivative (2, 4.1 mg from 655 g soybean curd residues), and its chemical structure was elucidated by field desorption mass spectrometry, electron ionization mass spectrometry, and nuclear magnetic resonance (NMR) analyses. After 48-h incubation, 40 µM diacetonamine hydrochloride (1b) exhibited sporulation-inducing activity with 35% sporulation frequency toward a Bacillus amyloliquefaciens wild-type strain (AHU 2170), whereas 40 µM diacetone acrylamide (3) showed 99% sporulation induction, which was much higher than that of 1b...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28532451/enhancing-poly-%C3%AE-glutamic-acid-production-in-bacillus-amyloliquefaciens-by-introducing-the-glutamate-synthesis-features-from-corynebacterium-glutamicum
#2
Jun Feng, Yufen Quan, Yanyan Gu, Fenghong Liu, Xiaozhong Huang, Haosheng Shen, Yulei Dang, Mingfeng Cao, Weixia Gao, Xiaoyun Lu, Yi Wang, Cunjiang Song, Shufang Wang
BACKGROUND: Poly-γ-glutamic acid (γ-PGA) is a valuable polymer with glutamate as its sole precursor. Enhancement of the intracellular glutamate synthesis is a very important strategy for the improvement of γ-PGA production, especially for those glutamate-independent γ-PGA producing strains. Corynebacterium glutamicum has long been used for industrial glutamate production and it exhibits some unique features for glutamate synthesis; therefore introduction of these metabolic characters into the γ-PGA producing strain might lead to increased intracellular glutamate availability, and thus ultimate γ-PGA production...
May 22, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28531554/efficacy-of-bacillus-subtilis-and-bacillus-amyloliquefaciens-in-the-control-of-aspergillus-parasiticus-growth-and-aflatoxins-production-on-pistachio
#3
Fatemeh Siahmoshteh, Ilenia Siciliano, Houda Banani, Zohreh Hamidi-Esfahani, Mehdi Razzaghi-Abyaneh, Maria Lodovica Gullino, Davide Spadaro
Pistachio (Pistacia vera) is an important nut for its economic, nutritional and health aspects but it can be contaminated by aflatoxigenic fungi in the field and during storage. Biological control could be considered as an alternative to chemical treatment. In this study, we evaluated the antifungal and anti-mycotoxigenic capability of two Bacillus spp. both in vitro and on pistachio kernels. In in vitro conditions, both strains were able to reduce the mycelial growth and they were able to degrade the four aflatoxins during the first three days after inoculation...
May 15, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28525654/molecular-detection-by-analysis-of-the-16s-rrna-gene-of-fecal-coliform-bacteria-from-the-two-korean-apodemus-species-apodemus-agrarius-and-a-peninsulae
#4
M M Rahman, K B Yoon, S J Lim, M G Jeon, H J Kim, H Y Kim, J Y Cho, H M Chae, Y C Park
Wild mouse feces can disseminate zoonotic microorganisms throughout a farm, which is a great threat to human health and can lead to economic loss through contaminated agricultural produce. To assess the microbial communities, especially fecal coliform bacteria, we used two methods. First, we isolated bacterial colonies onto the common media LB (lactose broth) agar, TSA (tryptic soy agar), and MRS (de Man, Rogosa, and Sharpe) agar, and then randomly select colonies from each plate and stocked them to the mother plate for genomic DNA isolation...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28521229/development-of-jerusalem-artichoke-resource-for-efficient-one-step-fermentation-of-poly-%C3%AE-glutamic-acid-using-a-novel-strain-bacillus-amyloliquefaciens-nx-2s
#5
Yibin Qiu, Yuanyuan Sha, Yatao Zhang, Zongqi Xu, Sha Li, Peng Lei, Zheng Xu, Xiaohai Feng, Hong Xu
This study aimed to develop non-food fermentation for the cost-effective production of poly-(γ-glutamic acid) (γ-PGA) using a novel strain of Bacillus amyloliquefaciens NX-2S. The new isolate assimilated inulin more efficiently than other carbohydrates from Jerusalem artichoke, without hydrolytic treatment. To investigate the effect of inulin on γ-PGA production, the transcript levels of γ-PGA synthetase genes (pgsB, pgsC, pgsA), regulatory genes (comA, degQ, degS), and the glutamic acid biosynthesis gene (glnA) were analyzed; inulin addition upregulated these key genes...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28481506/functional-silver-nanocomposites-as-broad-spectrum-antimicrobial-and-biofilm-disrupting-agents
#6
Qianqian Guo, Yu Zhao, Xiaomei Dai, Tianqi Zhang, Yunjian Yu, Xinge Zhang, Chaoxing Li
Biofilms' tolerance has become a serious clinical concern due to their formidable resistance to conventional antibiotics and prevalent virulence. Therefore, there is an urgent need to develop alternative antimicrobial agents to eradicate biofilms but avoid using antibiotics. Herein, we successfully developed polymer functional silver nanocomposites by reduction of silver nitrate in the presence of a biocompatible carbohydrate polymer and a membrane-disrupting cationic polymer. The nanocomposites presented effective antimicrobial activity against Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli) and Gram-positive bacteria (Staphylococcus aureus and Bacillus amyloliquefaciens)...
May 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28476667/paraprobiotic-preparation-from-bacillus-amyloliquefaciens-fptb16-modulates-immune-response-and-immune-relevant-gene-expression-in-catla-catla-hamilton-1822
#7
Sukham Tushiba Singh, Dibyendu Kamilya, Biswanath Kheti, Biswajyoti Bordoloi, Janmejay Parhi
The present study evaluated the paraprobiotic effect of heat-inactivated Bacillus amyloliquefaciens FPTB16 on immunological response and immune relevant gene expression in catla (Catla catla). Heat inactivation of viable cells of B. amyloliquefaciens was done at 60 °C for 2 h. For preparation of paraprobiotic supplemented diet, the heat-inactivated bacteria were added to the basal diet (control) at three different inclusion levels i.e., 10(7), 10(8) and 10(9) cells g(-1) diet. Fish (25.98 ± 2.57 g) were fed with these diets and various immune responses and immune relevant gene expressions were measured after 4 weeks of feeding...
May 2, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28460924/a-peptidomic-approach-for-the-identification-of-antioxidant-and-ace-inhibitory-peptides-in-sardinelle-protein-hydrolysates-fermented-by-bacillus-subtilis-a26-and-bacillus-amyloliquefaciens-an6
#8
Ines Jemil, Leticia Mora, Rim Nasri, Ola Abdelhedi, Maria-Concepción Aristoy, Mohamed Hajji, Moncef Nasri, Fidel Toldrá
Antioxidant and angiotensin I-converting enzyme (ACE)-inhibitory activities of sardinelle (Sardinella aurita) protein hydrolysates (SPHs) obtained by fermentation with Bacillus subtilis A26 (SPH-A26) and Bacillus amyloliquefaciens An6 (SPH-An6) were investigated. Both hydrolysates showed dose-dependent antioxidant activities evaluated using various in vitro antioxidant assays. Further, they were found to exhibit ACE-inhibitory activity. Peptides from SPH-A26 and SPH-An6 were analyzed by nESI-LC-MS/MS and approximately 800 peptides were identified...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28457958/effect-of-diurnal-photosynthetic-activity-on-the-fine-structure-of-amylopectin-from-normal-and-waxy-barley-starch
#9
Avi Goldstein, George Annor, Andreas Blennow, Eric Bertoft
The impact of diurnal photosynthetic activity on the fine structure of the amylopectin fraction of starch synthesized by normal barley (NBS) and waxy barley (WBS), the latter completely devoid of amylose biosynthesis, was determined following the cultivation under normal diurnal or constant light growing conditions. The amylopectin fine structures were analysed by characterizing its unit chain length profiles after enzymatic debranching as well as its φ,β-limit dextrins and its clusters and building blocks after their partial and complete hydrolysis with α-amylase from Bacillus amyloliquefaciens, respectively...
April 28, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28455550/contribution-of-native-phosphorous-solubilizing-bacteria-of-acid-soils-on-phosphorous-acquisition-in-peanut-arachis-hypogaea-l
#10
Madhusmita Pradhan, Ranjan Kumar Sahoo, Chinmay Pradhan, Narendra Tuteja, Santanu Mohanty
The present investigation analyzes the in vitro P solubilization [Ca-P, Al-P, Fe(II)-P, and Fe(III)-P] efficiency of native PSB strains from acid soils of Odisha and exploitation of the same through biofertilization in peanut (Arachis hypogaea L.) growth and P acquisition. One hundred six numbers of soil samples with pH ≤ 5.50 were collected from five districts of Odisha viz., Balasore, Cuttack, Khordha, Keonjhar, and Mayurbhanj. One bacterial isolate from each district were selected and analyzed for their P solubilization efficiency in National Botanical Research Institute Phosphate broths with Ca, Al, and Fe-complexed phosphates...
April 28, 2017: Protoplasma
https://www.readbyqxmd.com/read/28401458/purification-characterization-and-thermostability-improvement-of-xylanase-from-bacillus-amyloliquefaciens-and-its-application-in-pre-bleaching-of-kraft-pulp
#11
Sharad Kumar, Izharul Haq, Jyoti Prakash, Sudheer Kumar Singh, Shivaker Mishra, Abhay Raj
Xylanases have important industrial applications but are most extensively utilized in the pulp and paper industry as a pre-bleaching agent. We characterized a xylanase from Bacillus amyloliquefaciens strain SK-3 and studied it for kraft pulp bleaching. The purified enzyme had a molecular weight of ~50 kDa with optimal activity at pH 9.0 and 50 °C. The enzyme showed good activity retention (85%) after 2 h incubation at 50 °C and pH 9.0. This enzyme obeyed Michaelis-Menten kinetics with regard to beechwood xylan with K m and V max values of 5...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28392780/biocontrol-potentials-of-antimicrobial-peptide-producing-bacillus-species-multifaceted-antagonists-for-the-management-of-stem-rot-of-carnation-caused-by-sclerotinia-sclerotiorum
#12
S Vinodkumar, S Nakkeeran, P Renukadevi, V G Malathi
Bacillus species are widely exploited as biocontrol agents because of their efficiency in impeding various plant pathogens with multifaceted approach. In this study, Bacillus species were isolated from rhizosphere of various plants viz., carnations, cotton, turmeric, and bananas in Tamil Nadu state of India. Their potential to control the mycelial growth of Sclerotinia sclerotiorum was assessed in vitro by dual plate and partition plate techniques. B. amyloliquefaciens strain VB7 was much effective in inhibiting mycelial growth (45% inhibition of over control) and sclerotial production (100%)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28384588/production-of-bioactive-hydrolysate-using-protease-%C3%AE-glucosidase-and-%C3%AE-amylase-of-bacillus-spp-isolated-from-kinema
#13
Amit Kumar Rai, Samurailatpam Sanjukta, Rounak Chourasia, Ishani Bhat, Pardeep K Bhardwaj, Dinabandhu Sahoo
The aim of this study was the production of soybean bioactive hydrolysate using Bacillus spp. isolated from kinema. Totally 251 bacteria isolated from kinema samples, collected at different time period were screened for protease, β-glucosidase and α-amylase activities and further identified by ARDRA based grouping followed by analysis of 16S rRNA gene sequence similarity. The results showed that Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus licheniformis were the major Bacillus species. Twelve fermentative strains belonging to these groups and having high protease, α-amylase and β-glucosidase activity were used for solid state fermentation...
March 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28382029/probiotic-bacillus-amyloliquefaciens-sc06-induces-autophagy-to-protect-against-pathogens-in-macrophages
#14
Yanping Wu, Yang Wang, Hai Zou, Baikui Wang, Qiming Sun, Aikun Fu, Yuanyuan Wang, Yibing Wang, Xiaogang Xu, Weifen Li
Probiotics are increasingly applied in popularity in both humans and animals. Decades of research has revealed their beneficial effects, including the immune modulation in intestinal pathogens inhibition. Autophagy-a cellular process that involves the delivery of cytoplasmic proteins and organelles to the lysosome for degradation and recirculation-is essential to protect cells against bacterial pathogens. However, the mechanism of probiotics-mediated autophagy and its role in the elimination of pathogens are still unknown...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28376795/intramembrane-protease-rasp-boosts-protein-production-in-bacillus
#15
Jolanda Neef, Cristina Bongiorni, Vivianne J Goosens, Brian Schmidt, Jan Maarten van Dijl
BACKGROUND: The microbial cell factory Bacillus subtilis is a popular industrial platform for high-level production of secreted technical enzymes. Nonetheless, the effective secretion of particular heterologous enzymes remains challenging. Over the past decades various studies have tackled this problem, and major improvements were achieved by optimizing signal peptides or removing proteases involved in product degradation. On the other hand, serious bottlenecks in the protein export process per se remained enigmatic, especially for protein secretion at commercially significant levels by cells grown to high density...
April 4, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28365354/production-of-surfactin-from-waste-distillers-grains-by-co-culture-fermentation-of-two-bacillus-amyloliquefaciens-strains
#16
Yan Zhi, Qun Wu, Yan Xu
Distillers' grains (DGS), the main waste by-products of Chinese liquor industry, were used as substrate for surfactin production. Bacillus amyloliquefaciens MT45 could grow with DGS as sole carbon source to produce 1.04g/l surfactin. However, low amylase activity of MT45 limited sugar supply and the subsequent surfactin production. Therefore, MT45 was co-cultured with Bacillus strains that exhibited remarkable hydrolases activities. Surfactin yield increased by 50% when MT45 was co-cultured with B. amyloliquefaciens X82 that showed no product inhibition effect and did not develop extracellular matrix...
March 20, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28363654/production-of-exopolysaccharides-by-selected-bacillus-strains-optimization-of-media-composition-to-maximize-the-yield-and-structural-characterization
#17
Afshan Malick, Nastaran Khodaei, Noreddine Benkerroum, Salwa Karboune
Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3). Modest EPS yield of 6.7g/L was recorded for Bacillus amyloliquefaciens 23350 grown on M1, where the EPS produced was characterized by a low MW (<5kDa) and of being mainly glucans...
March 29, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28330279/immobilization-of%C3%A2-bacillus-amyloliquefaciens-sp1-and-its-alkaline-protease-in-various-matrices-for-effective-hydrolysis-of-casein
#18
Shiwani Guleria, Abhishek Walia, Anjali Chauhan, C K Shirkot
An extracellular alkaline protease producing B. amyloliquefaciens SP1 was isolated from apple rhizosphere having multifarious plant growth-promoting activities. B. amyloliquefaciens SP1 protease was immobilized using various concentrations of calcium alginate, agar and polyacrylamide to determine the optimum concentration for formation of the beads. Enzyme activity before immobilization (at 60 °C, pH 8.0 for 5 min) was 3580 µg/ml/min. The results of immobilization with various matrices revealed that 3 % calcium alginate (2829...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28321368/plant-growth-promoting-endophytic-bacteria-versus-pathogenic-infections-an-example-of-bacillus-amyloliquefaciens-rwl-1-and-fusarium-oxysporum-f-sp-lycopersici-in-tomato
#19
Raheem Shahzad, Abdul Latif Khan, Saqib Bilal, Sajjad Asaf, In-Jung Lee
Fungal pathogenic attacks are one of the major threats to the growth and productivity of crop plants. Currently, instead of synthetic fungicides, the use of plant growth-promoting bacterial endophytes has been considered intriguingly eco-friendly in nature. Here, we aimed to investigate the in vitro and in vivo antagonistic approach by using seed-borne endophytic Bacillus amyloliquefaciens RWL-1 against pathogenic Fusarium oxysporum f. sp. lycopersici. The results revealed significant suppression of pathogenic fungal growth by Bacillus amyloliquefaciens in vitro...
2017: PeerJ
https://www.readbyqxmd.com/read/28291380/beneficial-rhizobacterium-bacillus-amyloliquefaciens-sqr9-induces-plant-salt-tolerance-through-spermidine-production
#20
Lin Chen, Yunpeng Liu, Gengwei Wu, Nan Zhang, Qirong Shen, Ruifu Zhang
The inoculation of plants with plant-growth-promoting rhizobacterium has been an effective strategy for enhancing plant salt tolerance to diminish the loss of agricultural productivity caused by salt stress; however, the signal transmitted from bacteria to the plant under salt stress is poorly understood. In this study, the salt tolerance of Arabidopsis thaliana and Zea mays was enhanced by inoculation with Bacillus amyloliquefaciens SQR9. Using dialysis bags with different molecular weight cutoffs, we sorted through the molecules secreted by SQR9 and found that spermidine is responsible for enhancing plant salt tolerance...
May 2017: Molecular Plant-microbe Interactions: MPMI
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