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Bacillus amyloliquefaciens

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https://www.readbyqxmd.com/read/28401458/purification-characterization-and-thermostability-improvement-of-xylanase-from-bacillus-amyloliquefaciens-and-its-application-in-pre-bleaching-of-kraft-pulp
#1
Sharad Kumar, Izharul Haq, Jyoti Prakash, Sudheer Kumar Singh, Shivaker Mishra, Abhay Raj
Xylanases have important industrial applications but are most extensively utilized in the pulp and paper industry as a pre-bleaching agent. We characterized a xylanase from Bacillus amyloliquefaciens strain SK-3 and studied it for kraft pulp bleaching. The purified enzyme had a molecular weight of ~50 kDa with optimal activity at pH 9.0 and 50 °C. The enzyme showed good activity retention (85%) after 2 h incubation at 50 °C and pH 9.0. This enzyme obeyed Michaelis-Menten kinetics with regard to beechwood xylan with K m and V max values of 5...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28392780/biocontrol-potentials-of-antimicrobial-peptide-producing-bacillus-species-multifaceted-antagonists-for-the-management-of-stem-rot-of-carnation-caused-by-sclerotinia-sclerotiorum
#2
S Vinodkumar, S Nakkeeran, P Renukadevi, V G Malathi
Bacillus species are widely exploited as biocontrol agents because of their efficiency in impeding various plant pathogens with multifaceted approach. In this study, Bacillus species were isolated from rhizosphere of various plants viz., carnations, cotton, turmeric, and bananas in Tamil Nadu state of India. Their potential to control the mycelial growth of Sclerotinia sclerotiorum was assessed in vitro by dual plate and partition plate techniques. B. amyloliquefaciens strain VB7 was much effective in inhibiting mycelial growth (45% inhibition of over control) and sclerotial production (100%)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28384588/production-of-bioactive-hydrolysate-using-protease-%C3%AE-glucosidase-and-%C3%AE-amylase-of-bacillus-spp-isolated-from-kinema
#3
Amit Kumar Rai, Samurailatpam Sanjukta, Rounak Chourasia, Ishani Bhat, Pardeep K Bhardwaj, Dinabandhu Sahoo
The aim of this study was the production of soybean bioactive hydrolysate using Bacillus spp. isolated from kinema. Totally 251 bacteria isolated from kinema samples, collected at different time period were screened for protease, β-glucosidase and α-amylase activities and further identified by ARDRA based grouping followed by analysis of 16S rRNA gene sequence similarity. The results showed that Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus licheniformis were the major Bacillus species. Twelve fermentative strains belonging to these groups and having high protease, α-amylase and β-glucosidase activity were used for solid state fermentation...
March 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28382029/probiotic-bacillus-amyloliquefaciens-sc06-induces-autophagy-to-protect-against-pathogens-in-macrophages
#4
Yanping Wu, Yang Wang, Hai Zou, Baikui Wang, Qiming Sun, Aikun Fu, Yuanyuan Wang, Yibing Wang, Xiaogang Xu, Weifen Li
Probiotics are increasingly applied in popularity in both humans and animals. Decades of research has revealed their beneficial effects, including the immune modulation in intestinal pathogens inhibition. Autophagy-a cellular process that involves the delivery of cytoplasmic proteins and organelles to the lysosome for degradation and recirculation-is essential to protect cells against bacterial pathogens. However, the mechanism of probiotics-mediated autophagy and its role in the elimination of pathogens are still unknown...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28376795/intramembrane-protease-rasp-boosts-protein-production-in-bacillus
#5
Jolanda Neef, Cristina Bongiorni, Vivianne J Goosens, Brian Schmidt, Jan Maarten van Dijl
BACKGROUND: The microbial cell factory Bacillus subtilis is a popular industrial platform for high-level production of secreted technical enzymes. Nonetheless, the effective secretion of particular heterologous enzymes remains challenging. Over the past decades various studies have tackled this problem, and major improvements were achieved by optimizing signal peptides or removing proteases involved in product degradation. On the other hand, serious bottlenecks in the protein export process per se remained enigmatic, especially for protein secretion at commercially significant levels by cells grown to high density...
April 4, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28365354/production-of-surfactin-from-waste-distillers-grains-by-co-culture-fermentation-of-two-bacillus-amyloliquefaciens-strains
#6
Yan Zhi, Qun Wu, Yan Xu
Distillers' grains (DGS), the main waste by-products of Chinese liquor industry, were used as substrate for surfactin production. Bacillus amyloliquefaciens MT45 could grow with DGS as sole carbon source to produce 1.04g/l surfactin. However, low amylase activity of MT45 limited sugar supply and the subsequent surfactin production. Therefore, MT45 was co-cultured with Bacillus strains that exhibited remarkable hydrolases activities. Surfactin yield increased by 50% when MT45 was co-cultured with B. amyloliquefaciens X82 that showed no product inhibition effect and did not develop extracellular matrix...
March 20, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28363654/production-of-exopolysaccharides-by-selected-bacillus-strains-optimization-of-media-composition-to-maximize-the-yield-and-structural-characterization
#7
Afshan Malick, Nastaran Khodaei, Noreddine Benkerroum, Salwa Karboune
Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3). Modest EPS yield of 6.7g/L was recorded for Bacillus amyloliquefaciens 23350 grown on M1, where the EPS produced was characterized by a low MW (<5kDa) and of being mainly glucans...
March 28, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28330279/immobilization-of%C3%A2-bacillus-amyloliquefaciens-sp1-and-its-alkaline-protease-in-various-matrices-for-effective-hydrolysis-of-casein
#8
Shiwani Guleria, Abhishek Walia, Anjali Chauhan, C K Shirkot
An extracellular alkaline protease producing B. amyloliquefaciens SP1 was isolated from apple rhizosphere having multifarious plant growth-promoting activities. B. amyloliquefaciens SP1 protease was immobilized using various concentrations of calcium alginate, agar and polyacrylamide to determine the optimum concentration for formation of the beads. Enzyme activity before immobilization (at 60 °C, pH 8.0 for 5 min) was 3580 µg/ml/min. The results of immobilization with various matrices revealed that 3 % calcium alginate (2829...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28321368/plant-growth-promoting-endophytic-bacteria-versus-pathogenic-infections-an-example-of-bacillus-amyloliquefaciens-rwl-1-and-fusarium-oxysporum-f-sp-lycopersici-in-tomato
#9
Raheem Shahzad, Abdul Latif Khan, Saqib Bilal, Sajjad Asaf, In-Jung Lee
Fungal pathogenic attacks are one of the major threats to the growth and productivity of crop plants. Currently, instead of synthetic fungicides, the use of plant growth-promoting bacterial endophytes has been considered intriguingly eco-friendly in nature. Here, we aimed to investigate the in vitro and in vivo antagonistic approach by using seed-borne endophytic Bacillus amyloliquefaciens RWL-1 against pathogenic Fusarium oxysporum f. sp. lycopersici. The results revealed significant suppression of pathogenic fungal growth by Bacillus amyloliquefaciens in vitro...
2017: PeerJ
https://www.readbyqxmd.com/read/28291380/beneficial-rhizobacterium-bacillus-amyloliquefaciens-sqr9-induces-plant-salt-tolerance-through-spermidine-production
#10
Lin Chen, Yunpeng Liu, Gengwei Wu, Nan Zhang, Qirong Shen, Ruifu Zhang
The inoculation of plants with plant-growth-promoting rhizobacterium has been an effective strategy for enhancing plant salt tolerance to diminish the loss of agricultural productivity caused by salt stress; however, the signal transmitted from bacteria to the plant under salt stress is poorly understood. In this study, the salt tolerance of Arabidopsis thaliana and Zea mays was enhanced by inoculation with Bacillus amyloliquefaciens SQR9. Using dialysis bags with different molecular weight cutoffs, we sorted through the molecules secreted by SQR9 and found that spermidine is responsible for enhancing plant salt tolerance...
April 20, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28244029/effects-of-probiotic-bacillus-as-a-substitute-for-antibiotics-on-antioxidant-capacity-and-intestinal-autophagy-of-piglets
#11
Yang Wang, Yanping Wu, Baikui Wang, Xuefang Cao, Aikun Fu, Yali Li, Weifen Li
The objective of this study was to evaluate effects of probiotic Bacillus amyloliquefaciens (Ba) as a substitute for antibiotics on growth performance, antioxidant ability and intestinal autophagy of piglets. Ninety piglets were divided into three groups: G1 (containing 150 mg/Kg aureomycin in the diet); G2 (containing 75 mg/Kg aureomycin and 1 × 10(8) cfu/Kg Ba in the diet); G3 (containing 2 × 10(8) cfu/Kg Ba in the diet without any antibiotics). Each treatment had three replications of ten pigs per pen...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28242930/beneficial-effects-of-fermented-sardinelle-protein-hydrolysates-on-hypercaloric-diet-induced-hyperglycemia-oxidative-stress-and-deterioration-of-kidney-function-in-wistar-rats
#12
Ines Jemil, Rim Nasri, Ola Abdelhedi, Maria-Concepción Aristoy, Rabeb Ben Slama-Ben Salem, Choumous Kallel, Rim Marrekchi, Kamel Jamoussi, Abdelfattah ElFeki, Mohamed Hajji, Fidel Toldrá, Moncef Nasri
This study investigated the potential effects of fermented sardinelle protein hydrolysates (FSPHs) obtained by two proteolytic bacteria, Bacillus subtilis A26 (FSPH-A26) and Bacillus amyloliquefaciens An6 (FSPH-An6), on hypercaloric diet (HCD) induced hyperglycemia and oxidative stress in rats. Effects of FSPHs on blood glucose level, glucose tolerance, α-amylase activity and hepatic glycogen content were investigated, as well as their effect on the oxidative stress state. Biochemical findings revealed that, while undigested sardinelle proteins did not exhibit hypoglycemic activity, oral administration of FSPHs to HCD-fed rats reduced significantly α-amylase activity as well as glycemia and hepatic glycogen levels...
February 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28219523/combinatorial-effect-of-mutagenesis-and-medium-component-optimization-on-bacillus-amyloliquefaciens-antifungal-activity-and-efficacy-in-eradicating-botrytis-cinerea
#13
Fatma Masmoudi, Saoussen Ben Khedher, Amel Kamoun, Nabil Zouari, Slim Tounsi, Mohamed Trigui
This work is directed towards Bacillus amyloliquefaciens strain BLB371 metabolite production for biocontrol of fungal phytopathogens. In order to maximise antifungal metabolite production by this strain, two approaches were combined: random mutagenesis and medium component optimization. After three rounds of mutagenesis, a hyper active mutant, named M3-7, was obtained. It produces 7 fold more antifungal metabolites (1800AU/mL) than the wild strain in MC medium. A hybrid design was applied to optimise a new medium to enhance antifungal metabolite production by M3-7...
April 2017: Microbiological Research
https://www.readbyqxmd.com/read/28219382/high-level-extracellular-protein-production-in-bacillus-subtilis-using-an-optimized-dual-promoter-expression-system
#14
Kang Zhang, Lingqia Su, Xuguo Duan, Lina Liu, Jing Wu
BACKGROUND: We recently constructed a Bacillus subtilis strain (CCTCC M 2016536) from which we had deleted the srfC, spoIIAC, nprE, aprE and amyE genes. This strain is capable of robust recombinant protein production and amenable to high-cell-density fermentation. Because the promoter is among the factors that influence the production of target proteins, optimization of the initial promoter, PamyQ from Bacillus amyloliquefaciens, should improve protein expression using this strain. This study was undertaken to develop a new, high-level expression system in B...
February 20, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28213029/antifungal-effect-of-volatile-organic-compounds-produced-by-bacillus-amyloliquefaciens-cpa-8-against-fruit-pathogen-decays-of-cherry
#15
A Gotor-Vila, N Teixidó, A Di Francesco, J Usall, L Ugolini, R Torres, M Mari
The present work focuses on the antifungal effect of volatile organic compounds (VOCs) produced by Bacillus amyloliquefaciens CPA-8 against Monilinia laxa, M. fructicola and Botrytis cinera, three postharvest fruit pathogens of sweet cherry fruit. VOCs were evaluated with a double petri dish assay against mycelial and colony growth of target pathogens. For this purpose, CPA-8 was grown on different media and cultured for 24 and 48 h at 30 °C before assays. Data showed that mycelial growth inhibition was higher when CPA-8 was grown on Tryptone Soya Agar (TSA) while no differences were generally observed when CPA-8 was cultured for either, 24 and 48 h...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28204884/selection-of-bacillus-species-for-targeted-in-situ-release-of-prebiotic-galacto-rhamnogalacturonan-from-potato-pulp-in-piglets
#16
Carsten Jers, Mikael L Strube, Mette D Cantor, Bea K K Nielsen, Ole B Sørensen, Mette Boye, Anne S Meyer
We have previously shown that galacto-rhamnogalacturonan fibers can be enzymatically extracted from potato pulp and that these fibers have potential for exerting a prebiotic effect in piglets. The spore-forming Bacillus species are widely used as probiotics in feed supplements for pigs. In this study, we evaluated the option for further functionalizing Bacillus feed supplements by selecting strains possessing the enzymes required for extraction of the potentially prebiotic fibers. We established that it would require production and secretion of pectin lyase and/or polygalacturonase but no or limited secretion of galactanase and β-galactosidase...
February 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28197690/improvement-of-levan-production-in-bacillus-amyloliquefaciens-through-metabolic-optimization-of-regulatory-elements
#17
Yanyan Gu, Jiayi Zheng, Jun Feng, Mingfeng Cao, Weixia Gao, Yufen Quan, Yulei Dang, Yi Wang, Shufang Wang, Cunjiang Song
Levan is a functional homopolymer of fructose with considerable applications in food, pharmaceutical and cosmetic industries. To improve the levan production in Bacillus amyloliquefaciens, the regulatory elements of sacB (encoding levansucrase) expression and levansucrase secretion were optimized. Four heterologous promoters were evaluated for sacB expression, and the Pgrac promoter led to the highest level for both sacB transcription and levansucrase enzyme activity. The levan production in the corresponding recombinant strain ΔLP-pHTPgrac reached 30...
February 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28197131/bacillus-spp-isolated-from-puba-as-a-source-of-biosurfactants-and-antimicrobial-lipopeptides
#18
Karla J Perez, Jaime Dos Santos Viana, Fernanda C Lopes, Jamile Q Pereira, Daniel M Dos Santos, Jamil S Oliveira, Renata V Velho, Silvia M Crispim, Jacques R Nicoli, Adriano Brandelli, Regina M D Nardi
Several products of industrial interest are produced by Bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. This study aimed to investigate the potential of two bacterial isolates (P5 and C3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. Phylogenetic analyses showed close relation of isolates P5 and C3 with Bacillus amyloliquefaciens and Bacillus thuringiensis, respectively...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28191883/loap-is-a-broadly-conserved-antiterminator-protein-that-regulates-antibiotic-gene-clusters-in-bacillus-amyloliquefaciens
#19
Jonathan R Goodson, Steven Klupt, Chengxi Zhang, Paul Straight, Wade C Winkler
A valuable resource available in the search for new natural products is the diverse microbial life that spans the planet. A large subset of these microorganisms synthesize complex specialized metabolites exhibiting biomedically important activities. A limiting step to the characterization of these compounds is an elucidation of the genetic regulatory mechanisms that oversee their production. Although proteins that control transcription initiation of specialized metabolite gene clusters have been identified, those affecting transcription elongation have not been broadly investigated...
February 13, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28176460/application-of-bacteria-from-non-cultivated-plants-to-promote-growth-alter-root-architecture-and-alleviate-salt-stress-of-cotton
#20
I Irizarry, J F White
AIMS: Cotton seeds are frequently treated with acid to remove fibres and reduce seed-transmitted diseases. This process also eliminates beneficial bacteria on the seed surface. The goal of this research was to seek and apply beneficial bacteria to acid delinted cotton seeds to evaluate their growth-promoting and salt stress alleviating effects in seedlings. METHODS AND RESULTS: Bacteria were isolated from non-cultivated plants in the Malvaceae. Seeds were collected from Portia tree (Thespesia populnea) and wild cotton (Gossypium hirsutum) from coastal and arid areas of Puerto Rico...
April 2017: Journal of Applied Microbiology
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