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Bacillus amyloliquefaciens

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https://www.readbyqxmd.com/read/28330279/immobilization-of%C3%A2-bacillus-amyloliquefaciens-sp1-and-its-alkaline-protease-in-various-matrices-for-effective-hydrolysis-of-casein
#1
Shiwani Guleria, Abhishek Walia, Anjali Chauhan, C K Shirkot
An extracellular alkaline protease producing B. amyloliquefaciens SP1 was isolated from apple rhizosphere having multifarious plant growth-promoting activities. B. amyloliquefaciens SP1 protease was immobilized using various concentrations of calcium alginate, agar and polyacrylamide to determine the optimum concentration for formation of the beads. Enzyme activity before immobilization (at 60 °C, pH 8.0 for 5 min) was 3580 µg/ml/min. The results of immobilization with various matrices revealed that 3 % calcium alginate (2829...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28321368/plant-growth-promoting-endophytic-bacteria-versus-pathogenic-infections-an-example-of-bacillus-amyloliquefaciens-rwl-1-and-fusarium-oxysporum-f-sp-lycopersici-in-tomato
#2
Raheem Shahzad, Abdul Latif Khan, Saqib Bilal, Sajjad Asaf, In-Jung Lee
Fungal pathogenic attacks are one of the major threats to the growth and productivity of crop plants. Currently, instead of synthetic fungicides, the use of plant growth-promoting bacterial endophytes has been considered intriguingly eco-friendly in nature. Here, we aimed to investigate the in vitro and in vivo antagonistic approach by using seed-borne endophytic Bacillus amyloliquefaciens RWL-1 against pathogenic Fusarium oxysporum f. sp. lycopersici. The results revealed significant suppression of pathogenic fungal growth by Bacillus amyloliquefaciens in vitro...
2017: PeerJ
https://www.readbyqxmd.com/read/28291380/beneficial-rhizobacterium-bacillus-amyloliquefaciens-sqr9-induces-plant-salt-tolerance-through-spermidine-production
#3
Lin Chen, Yunpeng Liu, Gengwei Wu, Nan Zhang, Qi-Rong Shen, Ruifu Zhang
The inoculation of plants with plant growth-promoting rhizobacterium (PGPR) has been an effective strategy for enhancing plant salt tolerance to diminish the loss of agricultural productivity caused by salt stress, but the signal transmitted from bacteria to the plant under salt stress is poorly understood. In this study, the salt tolerance of Arabidopsis thaliana and Zea mays was enhanced by inoculation with Bacillus amyloliquefaciens SQR9. Using dialysis bags with different molecular weight cutoffs, we sorted through the molecules secreted by SQR9 and found that spermidine is responsible for enhancing plant salt tolerance...
March 14, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28244029/effects-of-probiotic-bacillus-as-a-substitute-for-antibiotics-on-antioxidant-capacity-and-intestinal-autophagy-of-piglets
#4
Yang Wang, Yanping Wu, Baikui Wang, Xuefang Cao, Aikun Fu, Yali Li, Weifen Li
The objective of this study was to evaluate effects of probiotic Bacillus amyloliquefaciens (Ba) as a substitute for antibiotics on growth performance, antioxidant ability and intestinal autophagy of piglets. Ninety piglets were divided into three groups: G1 (containing 150 mg/Kg aureomycin in the diet); G2 (containing 75 mg/Kg aureomycin and 1 × 10(8) cfu/Kg Ba in the diet); G3 (containing 2 × 10(8) cfu/Kg Ba in the diet without any antibiotics). Each treatment had three replications of ten pigs per pen...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28242930/beneficial-effects-of-fermented-sardinelle-protein-hydrolysates-on-hypercaloric-diet-induced-hyperglycemia-oxidative-stress-and-deterioration-of-kidney-function-in-wistar-rats
#5
Ines Jemil, Rim Nasri, Ola Abdelhedi, Maria-Concepción Aristoy, Rabeb Ben Slama-Ben Salem, Choumous Kallel, Rim Marrekchi, Kamel Jamoussi, Abdelfattah ElFeki, Mohamed Hajji, Fidel Toldrá, Moncef Nasri
This study investigated the potential effects of fermented sardinelle protein hydrolysates (FSPHs) obtained by two proteolytic bacteria, Bacillus subtilis A26 (FSPH-A26) and Bacillus amyloliquefaciens An6 (FSPH-An6), on hypercaloric diet (HCD) induced hyperglycemia and oxidative stress in rats. Effects of FSPHs on blood glucose level, glucose tolerance, α-amylase activity and hepatic glycogen content were investigated, as well as their effect on the oxidative stress state. Biochemical findings revealed that, while undigested sardinelle proteins did not exhibit hypoglycemic activity, oral administration of FSPHs to HCD-fed rats reduced significantly α-amylase activity as well as glycemia and hepatic glycogen levels...
February 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28219523/combinatorial-effect-of-mutagenesis-and-medium-component-optimization-on-bacillus-amyloliquefaciens-antifungal-activity-and-efficacy-in-eradicating-botrytis-cinerea
#6
Fatma Masmoudi, Saoussen Ben Khedher, Amel Kamoun, Nabil Zouari, Slim Tounsi, Mohamed Trigui
This work is directed towards Bacillus amyloliquefaciens strain BLB371 metabolite production for biocontrol of fungal phytopathogens. In order to maximise antifungal metabolite production by this strain, two approaches were combined: random mutagenesis and medium component optimization. After three rounds of mutagenesis, a hyper active mutant, named M3-7, was obtained. It produces 7 fold more antifungal metabolites (1800AU/mL) than the wild strain in MC medium. A hybrid design was applied to optimise a new medium to enhance antifungal metabolite production by M3-7...
April 2017: Microbiological Research
https://www.readbyqxmd.com/read/28219382/high-level-extracellular-protein-production-in-bacillus-subtilis-using-an-optimized-dual-promoter-expression-system
#7
Kang Zhang, Lingqia Su, Xuguo Duan, Lina Liu, Jing Wu
BACKGROUND: We recently constructed a Bacillus subtilis strain (CCTCC M 2016536) from which we had deleted the srfC, spoIIAC, nprE, aprE and amyE genes. This strain is capable of robust recombinant protein production and amenable to high-cell-density fermentation. Because the promoter is among the factors that influence the production of target proteins, optimization of the initial promoter, PamyQ from Bacillus amyloliquefaciens, should improve protein expression using this strain. This study was undertaken to develop a new, high-level expression system in B...
February 20, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28213029/antifungal-effect-of-volatile-organic-compounds-produced-by-bacillus-amyloliquefaciens-cpa-8-against-fruit-pathogen-decays-of-cherry
#8
A Gotor-Vila, N Teixidó, A Di Francesco, J Usall, L Ugolini, R Torres, M Mari
The present work focuses on the antifungal effect of volatile organic compounds (VOCs) produced by Bacillus amyloliquefaciens CPA-8 against Monilinia laxa, M. fructicola and Botrytis cinera, three postharvest fruit pathogens of sweet cherry fruit. VOCs were evaluated with a double petri dish assay against mycelial and colony growth of target pathogens. For this purpose, CPA-8 was grown on different media and cultured for 24 and 48 h at 30 °C before assays. Data showed that mycelial growth inhibition was higher when CPA-8 was grown on Tryptone Soya Agar (TSA) while no differences were generally observed when CPA-8 was cultured for either, 24 and 48 h...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28204884/selection-of-bacillus-species-for-targeted-in-situ-release-of-prebiotic-galacto-rhamnogalacturonan-from-potato-pulp-in-piglets
#9
Carsten Jers, Mikael L Strube, Mette D Cantor, Bea K K Nielsen, Ole B Sørensen, Mette Boye, Anne S Meyer
We have previously shown that galacto-rhamnogalacturonan fibers can be enzymatically extracted from potato pulp and that these fibers have potential for exerting a prebiotic effect in piglets. The spore-forming Bacillus species are widely used as probiotics in feed supplements for pigs. In this study, we evaluated the option for further functionalizing Bacillus feed supplements by selecting strains possessing the enzymes required for extraction of the potentially prebiotic fibers. We established that it would require production and secretion of pectin lyase and/or polygalacturonase but no or limited secretion of galactanase and β-galactosidase...
February 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28197690/improvement-of-levan-production-in-bacillus-amyloliquefaciens-through-metabolic-optimization-of-regulatory-elements
#10
Yanyan Gu, Jiayi Zheng, Jun Feng, Mingfeng Cao, Weixia Gao, Yufen Quan, Yulei Dang, Yi Wang, Shufang Wang, Cunjiang Song
Levan is a functional homopolymer of fructose with considerable applications in food, pharmaceutical and cosmetic industries. To improve the levan production in Bacillus amyloliquefaciens, the regulatory elements of sacB (encoding levansucrase) expression and levansucrase secretion were optimized. Four heterologous promoters were evaluated for sacB expression, and the Pgrac promoter led to the highest level for both sacB transcription and levansucrase enzyme activity. The levan production in the corresponding recombinant strain ΔLP-pHTPgrac reached 30...
February 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28197131/bacillus-spp-isolated-from-puba-as-a-source-of-biosurfactants-and-antimicrobial-lipopeptides
#11
Karla J Perez, Jaime Dos Santos Viana, Fernanda C Lopes, Jamile Q Pereira, Daniel M Dos Santos, Jamil S Oliveira, Renata V Velho, Silvia M Crispim, Jacques R Nicoli, Adriano Brandelli, Regina M D Nardi
Several products of industrial interest are produced by Bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. This study aimed to investigate the potential of two bacterial isolates (P5 and C3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. Phylogenetic analyses showed close relation of isolates P5 and C3 with Bacillus amyloliquefaciens and Bacillus thuringiensis, respectively...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28191883/loap-is-a-broadly-conserved-antiterminator-protein-that-regulates-antibiotic-gene-clusters-in-bacillus-amyloliquefaciens
#12
Jonathan R Goodson, Steven Klupt, Chengxi Zhang, Paul Straight, Wade C Winkler
A valuable resource available in the search for new natural products is the diverse microbial life that spans the planet. A large subset of these microorganisms synthesize complex specialized metabolites exhibiting biomedically important activities. A limiting step to the characterization of these compounds is an elucidation of the genetic regulatory mechanisms that oversee their production. Although proteins that control transcription initiation of specialized metabolite gene clusters have been identified, those affecting transcription elongation have not been broadly investigated...
February 13, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28176460/application-of-bacteria-from-non-cultivated-plants-to-promote-growth-alter-root-architecture-and-alleviate-salt-stress-of-cotton
#13
I Irizarry, J F White
AIMS: Cotton seeds are frequently treated with acid to remove fibres and reduce seed-transmitted diseases. This process also eliminates beneficial bacteria on the seed surface. The goal of this research was to seek and apply beneficial bacteria to acid delinted cotton seeds to evaluate their growth-promoting and salt stress alleviating effects in seedlings. METHODS AND RESULTS: Bacteria were isolated from non-cultivated plants in the Malvaceae. Seeds were collected from Portia tree (Thespesia populnea) and wild cotton (Gossypium hirsutum) from coastal and arid areas of Puerto Rico...
April 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28164794/genomic-and-metabolic-traits-endow-bacillus-velezensis-cc09-with-a-potential-biocontrol-agent-in-control-of-wheat-powdery-mildew-disease
#14
Xun-Chao Cai, Chang-Hong Liu, Bao-Tong Wang, Ya-Rong Xue
Bacillus velezensis CC09, which was isolated from healthy leaves of Cinnamomum camphora and previously identified as Bacillus amyloliquefaciens CC09, shows great potential as a new biocontrol agent, in control of many phytopathogenic diseases. To extend our understanding of the potential antifungal capacities, we did a whole genome analysis of strain CC09. Result shows that strain CC09 has a relatively large genome size (4.17Mb) with an average GC content of 46.1%, and 4021 predicted genes. Thirteen secondary metabolites encoding clusters have been identified within the genome of B...
March 2017: Microbiological Research
https://www.readbyqxmd.com/read/28163698/bacillus-amyloliquefaciens-bacillus-velezensis-and-bacillus-siamensis-form-an-operational-group-b-amyloliquefaciens-within-the-b-subtilis-species-complex
#15
Ben Fan, Jochen Blom, Hans-Peter Klenk, Rainer Borriss
The plant growth promoting model bacterium FZB42(T) was proposed as the type strain of Bacillus amyloliquefaciens subsp. plantarum (Borriss et al., 2011), but has been recently recognized as being synonymous to Bacillus velezensis due to phylogenomic analysis (Dunlap C. et al., 2016). However, until now, majority of publications consider plant-associated close relatives of FZB42 still as "B. amyloliquefaciens." Here, we reinvestigated the taxonomic status of FZB42 and related strains in its context to the free-living soil bacterium DSM7(T), the type strain of B...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28154555/key-impact-of-an-uncommon-plasmid-on-bacillus-amyloliquefaciens-subsp-plantarum-s499-developmental-traits-and-lipopeptide-production
#16
Giulia Molinatto, Laurent Franzil, Sébastien Steels, Gerardo Puopolo, Ilaria Pertot, Marc Ongena
The rhizobacterium Bacillus amyloliquefaciens subsp. plantarum S499 (S499) is particularly efficient in terms of the production of cyclic lipopeptides, which are responsible for the high level of plant disease protection provided by this strain. Sequencing of the S499 genome has highlighted genetic differences and similarities with the closely related rhizobacterium B. amyloliquefaciens subsp. plantarum FZB42 (FZB42). More specifically, a rare 8008 bp plasmid (pS499) harboring a rap-phr cassette constitutes a major distinctive element between S499 and FZB42...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28153674/high-performance-thin-layer-chromatography-hptlc-for-the-simultaneous-quantification-of-the-cyclic-lipopeptides-surfactin-iturin-a-and-fengycin-in-culture-samples-of-bacillus-species
#17
Mareen Geissler, Claudia Oellig, Karin Moss, Wolfgang Schwack, Marius Henkel, Rudolf Hausmann
A high-performance thin-layer chromatography method has been established for the identification and simultaneous quantification of the cyclic lipopeptides Surfactin, Iturin A and Fengycin in Bacillus culture samples. B. subtilis DSM 10(T), B. amyloliquefaciens DSM 7(T) and B. methylotrophicus DSM 23117 were used as model strains. Culture samples indicated that a sample pretreatment is necessary in order to run HPTLC analyses. A threefold extraction of the cell-free broth with the solvent chloroform/methanol (2:1, v/v) gave best results, when all three lipopeptides were included in the analysis...
November 10, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28131539/acetylation-and-deacetylation-for-sucralose-preparation-by-a-newly-isolated-bacillus-amyloliquefaciens-wzs01
#18
Jie Sun, Liang Chen, Bo Lou, Yanbing Bai, Xinjun Yu, Man Zhao, Zhao Wang
Sucralose is a non-nutritive artificial sweetener used in a broad range of foods and beverages. In the present study, Bacillus amyloliquefaciens WZS01 was isolated, identified, and used as a catalyst both in regioselective acylation and deacetylation for sucralose preparation. Bacterial cells were immobilized on polyurethane foam and utilized to synthesize sucrose-6-acetate regioselectively. The yield of sucrose-6-acetate was >95% with 60 mM sucrose after 22 h of reaction. Free cells could hydrolyze 75 mM sucralose-6-acetate to produce sucralose with >99% yield after 24 h of reaction...
January 25, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28130767/production-and-characterization-of-organic-solvent-tolerant-cellulase-from-bacillus-amyloliquefaciens-ak9-isolated-from-hot-spring
#19
Muhammad Irfan, Ammara Tayyab, Fariha Hasan, Samiullah Khan, Malik Badshah, Aamer Ali Shah
A cellulase-producing bacterium, designated as strain AK9, was isolated from a hot spring of Tatta Pani, Azad Kashmir, Pakistan. The bacterium was identified as Bacillus amyloliquefaciens through 16S rRNA sequencing. Cellulase from strain AK9 was able to liberate glucose from soluble cellulose and carboxymethyl cellulose (CMC). Enzyme was purified through size exclusion chromatography and a single band of ∼47 kDa was observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme was purified with recovery of 35...
January 27, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28124780/the-antibacterial-activity-of-e-coli-bacteriophage-lysin-lysep3-is-enhanced-by-fusing-the-bacillus-amyloliquefaciens-bacteriophage-endolysin-binding-domain-d8-to-the-c-terminal-region
#20
Shuang Wang, Jingmin Gu, Meng Lv, Zhimin Guo, Guangmou Yan, Ling Yu, Chongtao Du, Xin Feng, Wenyu Han, Changjiang Sun, Liancheng Lei
Bacteriophage endolysin is one of the most promising antibiotic substitutes, but in Gram-negative bacteria, the outer membrane prevents the lysin from hydrolyzing peptidoglycans and blocks the development of lysin applications. The prime strategy for new antibiotic substitutes is allowing lysin to access the peptidoglycan from outside of the bacteria by reformation of the lysin. In this study, the novel Escherichia coli (E. coli) phage lyase lysep3, which lacks outside-in catalytic ability, was fused with the N-terminal region of the Bacillus amyloliquefaciens lysin including its cell wall binding domain D8 through the best manner of protein fusion based on the predicted tertiary structure of lysep3-D8 to obtain an engineered lysin that can lyse bacteria from the outside...
January 26, 2017: Journal of Microbiology / the Microbiological Society of Korea
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