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Bacillus amyloliquefaciens

Yue Li, Hao Zhang, Weipeng Su, Zhixiong Ying, Yueping Chen, Lili Zhang, Zhaoxin Lu, Tian Wang
Background: The focus of recent research has been directed toward the probiotic potential of Bacillus amyloliquefaciens (BA) on the gut health of animals. However, little is known about BA's effects on piglets with intra-uterine growth retardation (IUGR). Therefore, this study investigated the effects of BA supplementation on the growth performance, intestinal morphology, inflammatory response, and microbiota of IUGR piglets. Methods: Eighteen litters of newborn piglets were selected at birth, with one normal birth weight (NBW) and two IUGR piglets in each litter (i...
2018: Journal of Animal Science and Biotechnology
M Rath, T R Mitchell, S E Gold
Volatile organic compounds (VOCs) produced by Plant Growth Promoting Rhizobacteria have recently been investigated due to their role in plant growth promotion and defense. Whereas some bacterial VOCs like 3-hydroxy-2-butanone (acetoin) and 2,3-butanediol produced by strains of Bacillus subtilis and Bacillus amyloliquefaciens promote plant growth, others like hydrogen cyanide and 3-phenylpropionic acid are phytotoxic, inhibiting plant growth. Bacillus mojavensis, a close relative of B. subtilis, is an endophytic bacterium of maize that has been shown to have antagonistic activity against the mycotoxigenic phytopathogen Fusarium verticillioides and growth promotion activity on maize seedlings...
March 2018: Microbiological Research
Ji-Eun Kang, Hyun-Do Kim, Soo-Young Park, Jae-Gu Pan, Jeong Hyun Kim, Do-Young Yum
Background and Aims: Commercial superoxide dismutase (SOD) is derived from melon extract and has a potential as a dietary supplement due to its beneficial anti-oxidative effects. We aimed to improve the productivity of SOD compared with plant SOD by using a generally-regarded-as-safe (GRAS) microorganism, Bacillus amyloliquefaciens, and assess its anti-oxidative effect using γ-radiation- and dextransulfate sodium (DSS)-induced oxidative models in mice. Methods: We identified the sodA gene encoding manganese-containing SODs (Mn-SOD) in B...
March 14, 2018: Journal of Crohn's & Colitis
Kiran R Gadhave, Paul F Devlin, Andreas Ebertz, Arabella Ross, Alan C Gange
The use of microbial inoculants containing plant growth-promoting rhizobacteria as a promoter of plant fitness and health is becoming increasingly popular in agriculture. However, whether and how these bacteria affect indigenous bacterial communities in field conditions is sparsely explored. We studied the effects of seed inoculation and field soil application of ubiquitous soil bacteria, B. cereus, B. subtilis, and B. amyloliquefaciens, on the diversity, evenness, and richness of endophytic bacterial communities in sprouting broccoli roots using high-throughput metagenome sequencing...
March 6, 2018: Microbial Ecology
Simon Caulier, Annika Gillis, Gil Colau, Florent Licciardi, Maxime Liépin, Nicolas Desoignies, Pauline Modrie, Anne Legrève, Jacques Mahillon, Claude Bragard
The world potato is facing major economic losses due to disease pressure and environmental concerns regarding pesticides use. This work aims at addressing these two issues by isolating indigenous bacteria that can be integrated into pest management strategies. More than 2,800 strains of Bacillus -like and Pseudomonas -like were isolated from several soils and substrates associated with potato agro-systems in Belgium. Screenings for antagonistic activities against the potato pathogens Alternaria solani, Fusarium solani (BCCM-MUCL 5492), Pectobacterium carotovorum (ATCC 15713), Phytophthora infestans (CRA-W10022) and Rhizoctonia solani (BCCM-MUCL 51929) were performed, allowing the selection of 52 Bacillus spp...
2018: Frontiers in Microbiology
Shashi Kant Bhatia, Jeong-Jun Yoon, Hyun-Joong Kim, Ju Won Hong, Yoon Gi Hong, Hun-Seok Song, Yu-Mi Moon, Jong-Min Jeon, Yun-Gon Kim, Yung-Hun Yang
Ralstonia eutropha is a well-known microbe reported for polyhydroxyalkonate (PHA) production, and unable to utilize sucrose as carbon source. Two strains, Ralstonia eutropha H16 and Ralstonia eutropha 5119 were co-cultured with sucrose hydrolyzing microbes (Bacillus subtilis and Bacillus amyloliquefaciens) for PHA production. Co-culture of B. subtilis:R. eutropha 5119 (BS:RE5) resulted in best PHA production (45% w/w dcw). Optimization of the PHA production process components through response surface resulted in sucrose: NH4 Cl:B...
February 15, 2018: Bioresource Technology
Ivelisse Irizarry, James F White
AIMS: Previous research demonstrated that applying Bacillus amyloliquefaciens to cotton seeds promotes growth, alters root architecture, and alleviates salt stress of cotton seedlings. This research was undertaken to further study the genetic responses elicited in cotton seedlings by this growth promoting bacterium. METHODS AND RESULTS: GeneChip microarrays and RT-qPCR were used to detect changes in gene expression in seedling roots inoculated with B. amyloliquefaciens...
February 23, 2018: Journal of Applied Microbiology
Marie Christine Uwamahoro, Richard Massicotte, Yves Hurtubise, François Gagné-Bourque, Akier Assanta Mafu, L'Hocine Yahia
Spore-forming pathogenic bacteria, such as Clostridium difficile , are associated with nosocomial infection, leading to the increased use of sporicidal disinfectants, which impacts socioeconomic costs. However, C. difficile can be prevented using microorganisms such as Bacillus amyloliquefaciens , a prophylactic agent that has been proven to be effective against it in recent tests or it can be controlled by sporicidal disinfectants. These disinfectants against spores should be evaluated according to a known and recommended standard...
2018: Frontiers in Public Health
Lingjuan Han, Taixun Pu, Xi Wang, Beiling Liu, Yonghong Wang, Juntao Feng, Xing Zhang
Response surface methodology (RSM) is a commonly used system to optimize cryoprotectants of biocontrol strains when they are subjected to preparations. Various kinds of cryoprotectants and centrifugal conditions were tested to improve the survival of biocontrol agents after freeze-drying. To determine the optimum levels of incorporation of three cryoprotectants (glucose, trehalose and xylitol) in the freeze-drying process of strain Bacillus amyloliquefaciens B1408, a range of experiments based on Box-Behnken Design (BBD) were conducted...
February 16, 2018: Cryobiology
Hang Ren, Ya-Ting Su, Xiao-Hua Guo
Some optimization techniques have been widely applied for spore fermentation based on the plate counting. This study optimized the culture medium for the spore production of Bacillus amyloliquefaciens BS-20 and investigated the feasibility of using a dipicolonic acid (DPA) fluorimetry assay as a simpler alternative to plate counting for evaluating spore yields. Through the single-factor experiment, the metal ions and agro-industrial raw materials that significantly enhanced spore production were determined...
February 16, 2018: AMB Express
Aradhana Mishra, Satyendra Pratap Singh, Sahil Mahfooz, Surendra Pratap Singh, Arpita Bhattacharya, Nishtha Mishra, C S Nautiyal
Endophytes have been explored to perform an important role in plant health. However, their effects on the host physiological function and disease management remains elusive. The present study was aimed to assess the potential of endophytes singly as well as in combination on various physiological parameters and systemic defense mechanism against Alternaria alternata in Withania somnifera (L.) Dunal. Seed priming with endophytic Bacillus amyloliquefaciens and Pseudomonas fluorescens individually and in combination demonstrated enhanced vigor index and germination rate...
February 16, 2018: Applied and Environmental Microbiology
Zongwang Ma, Jiangchun Hu
Certain Bacillus species have the capacity to produce cyclic lipopeptides and these lipopeptides are promising determinants contributing to the biocontrol of plant diseases. In the current study, a cyclic lipopeptide plipastatin A1 was isolated from the fermentation broth of a marine sediment-derived Bacillus amyloliquefaciens SH-B74 by the combination of solid-phase extraction and reversed-phase high-performance liquid chromatography, and its structure was identified by tandem mass spectrometry, high-resolution electro-spray ionization mass spectrometry, and gas chromatography-mass spectrometry together with nuclear magnetic resonance analysis...
February 2018: 3 Biotech
Hajeewaka C Mendis, Varghese P Thomas, Patrick Schwientek, Rauf Salamzade, Jung-Ting Chien, Pramuditha Waidyarathne, Joseph Kloepper, Leonardo De La Fuente
Bacillus amyloliquefaciens QST713 and B. firmus I-1582 are bacterial strains which are used as active ingredients of commercially-available soil application and seed treatment products Serenade® and VOTiVO®, respectively. These bacteria colonize plant roots promoting plant growth and offering protection against pathogens/pests. The objective of this study was to develop a qPCR protocol to quantitate the dynamics of root colonization by these two strains under field conditions. Primers and TaqMan® probes were designed based on genome comparisons of the two strains with publicly-available and unpublished bacterial genomes of the same species...
2018: PloS One
S Zhang, D D Li, F Zeng, Z H Zhu, P Song, M Zhao, J A Duan
A novel water-soluble flavonoid with good anti-bacterial activities, naringenin-6″-succl-7-O-glucoside (7-SGN), was synthesised. It was biotransformed from naringenin by Bacillus amyloliquefaciens FJ18 in aqueous miscible organic media, and characterised by LC-MS and NMR analysis. The solubility of 7-SGN in water was approximately 102 times higher than that of naringenin. These results demonstrated that both the water solubility and the anti-bacterial activity of 7-SGN were significantly improved.
February 15, 2018: Natural Product Research
Gwang Hun Park, Hun Min Song, Young Soo Kim, Yongho Jeon, Jin Suk Koo, Hyung Jin Jeong, Jin Boo Jeong
Microorganisms have been regarded as important sources of novel bioactive natural products. In this study, we evaluated the anti-cancer activity and the potential mechanism of Bacillus amyloliquefaciens AK-0 newly isolated from the rhizosphere soil of Korean ginseng. The ethyl acetate fraction from the culture medium of B. amyloliquefaciens AK-0 (EA-AK0) inhibited markedly the proliferation of human colorectal cancer cells such as HCT116, SW480, LoVo and HT-29. EA-AK0 effectively decreased cyclin D1 protein level in human colorectal cancer cells, while cyclin D1 mRNA level was not changed by EA-AK0 treatment...
June 1, 2017: Die Pharmazie
Anil S Prajapati, Vishakha A Pawar, Ketankumar J Panchal, Ankit P Sudhir, Bhaumik R Dave, Darshan H Patel, R B Subramanian
BACKGROUND: The aromatic residues of xylanase enzyme, W187, Y124, W144, Y128 and W63 of substrate binding pocket from Bacillus amyloliquefaciens were investigated for their role in substrate binding by homology modelling and sequence analysis. These residues are highly conserved and play an important role in substrate binding through steric hindrance. The substitution of these residues with alanine allows the enzyme to accommodate nonspecific substrates. RESULTS: Wild type and mutated genes were cloned and overexpressed in BL21...
February 13, 2018: BMC Biotechnology
Smail Ait Bahadou, Abderrahmane Ouijja, Abdelkarim Karfach, Abdessalem Tahiri, Rachid Lahlali
The effectiveness of antagonistic bacteria to control Erwinia amylovora was evaluated under in vitro and field conditions. Among 61 bacteria isolated from soil and flowers of fire blight host plants of different Moroccan areas, 20 bacterial isolates showed higher antagonistic activity against the pathogen during agar-diffusion-test, attached blossoms assay and in a bioassay on immature pear fruits. Effective isolates were identified by using biochemical tests and 16 S rRNA genes sequencing. These isolates were grouped into the following genera: Alcaligenes (ACBC1), Bacillus (CPa12, CPa2, HF6, JB2, LMR2, SF14, SF16, SP10, SP13, and SP18) Brevibacterium (SF3, SF4, SF7, and SF15), Pantoea (ACBC2, ACBP1, and ACBP2), Pseudomonas (SP9), and Serratia (HC4)...
February 8, 2018: Microbial Pathogenesis
Xuan Zhou, Nan Zhang, Liming Xia, Qing Li, Jiahui Shao, Qirong Shen, Ruifu Zhang
Efficient biofilm formation and root colonization capabilities facilitate the ability of beneficial plant rhizobacteria to promote plant growth and antagonize soil-borne pathogens. Biofilm formation by plant-beneficial Bacillus strains is triggered by environmental cues, including oxygen deficiency, but the pathways that sense these environmental signals and regulate biofilm formation have not been thoroughly elucidated. In this study, we showed that the ResDE two-component regulatory system in the plant growth-promoting rhizobacterium Bacillus amyloliquefaciens SQR9 senses the oxygen deficiency signal and regulates biofilm formation...
February 2, 2018: Applied and Environmental Microbiology
Jimmy Omony, Anne de Jong, Antonina O Krawczyk, Robyn T Eijlander, Oscar P Kuipers
Sporulation is a survival strategy, adapted by bacterial cells in response to harsh environmental adversities. The adaptation potential differs between strains and the variations may arise from differences in gene regulation. Gene networks are a valuable way of studying such regulation processes and establishing associations between genes. We reconstructed and compared sporulation gene co-expression networks (GCNs) of the model laboratory strain Bacillus subtilis 168 and the food-borne industrial isolate Bacillus amyloliquefaciens...
February 9, 2018: Microbial Genomics
Yogesh Devaraj, Savita Kumari Rajender, Prakash Motiram Halami
A serine protease with preference for fibrin protein was purified and characterized from B.amyloliquefaciens MCC2606, isolated from dosa batter. The protease was purified using ammonium sulphate precipitation, ion-exchange and gel filtration chromatography. The degradation activity of the protease towards the fibrin was significantly higher compared with other protein substrates in the study. The molecular weight of the CFR15-protease was estimated to be 32 kDa based on SDS-PAGE. The purified enzyme exhibited both fibrinolytic and fibrinogenolytic activity...
January 17, 2018: Preparative Biochemistry & Biotechnology
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