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Bacillus amyloliquefaciens

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https://www.readbyqxmd.com/read/28744833/evaluation-of-in-vivo-probiotic-efficiency-of-bacillus-amyloliquefaciens-in-labeo-rohita-challenged-by-pathogenic-strain-of-aeromonas-hydrophila-mtcc-1739
#1
Ankita Nandi, Goutam Banerjee, Suhas Kumar Dan, Koushik Ghosh, Arun Kumar Ray
Diseases in aquatic organisms, including fish, are a major concern in aquaculture production. In this present investigation, we have evaluated the beneficial effects of dietary Bacillus amyloliquefaciens CCF7 (GenBank Acc. No. KP256501) supplementation in rohu (Labeo rohita) challenged by a pathogenic strain of Aeromonas hydrophila MTCC 1739. Four experimental diets were formulated: control diet (no probiotics) and three experimental diets (different concentrations of probiotic candidate B. amyloliquefaciens CCF7 at 10(5) (T1), 10(7) (T2), 10(9) (T3) CFU/g)...
July 25, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28741666/assessment-of-the-potential-of-the-multi-enzyme-producer-bacillus-amyloliquefaciens-us573-as-alternative-feed-additive
#2
Ameny Farhat-Khemakhem, Monia Blibech, Ines Boukhris, Mohamed Makni, Hichem Chouayekh
BACKGROUND: Recently, probiotics have increasingly been used as feed additives in poultry diets as an alternative to antibiotic growth promoters fostering resistance development. RESULTS: This study was aimed at assessing the potential of Bacillus amyloliquefaciens US573 as a direct-fed microbial. The US573 strain was found to be free of harmful enzymatic activities and sensitive to antibiotics. In addition, it showed a good acid and bovine bile tolerance, high adhesion efficacy to chicken enterocytes, and an ability to form biofilms, which may favor its survival and persistence in the animal gastrointestinal tract...
July 25, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28733288/bacillomycin-d-produced-by-bacillus-amyloliquefaciens-is-involved-in-the-antagonistic-interaction-with-the-plant-pathogenic-fungus-fusarium-graminearum
#3
Qin Gu, Yang Yang, Qiming Yuan, Guangming Shi, Liming Wu, Zhiying Lou, Rong Huo, Huijun Wu, Rainer Borriss, Xuewen Gao
Fusarium graminearum (teleomorph: Ascomycota, Hypocreales, Gibberella, Gibberella zeae) is a destructive fungal pathogen that threatens the production and quality of wheat and barley worldwide. Controlling this toxin-producing pathogen is a significant challenge. In the present study, the commercially available strain Bacillus amyloliquefaciens (Bacteria, Firmicutes, Bacillales, Bacillus) FZB42 showed strong activity against F. graminearum The lipopeptide, bacillomycin D, produced by FZB42, was proven to contribute to the antifungal activity...
July 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28732724/recombinant-bacillus-amyloliquefaciens-xylanase-a-expressed-in-pichia-pastoris-and-generation-of-xylooligosaccharides-from-xylans-and-wheat-bran
#4
Ming-Qi Liu, Wen-Kang Huo, Xin Xu, Xiao-Yan Weng
In this study, BaxA (GenBank: KM624029), which encodes the Bacillus amyloliquefaciens xylanase A (BaxA), was highly expressed in Pichia pastoris GS115 under the control of the AOX1 promoter. The recombinant xylanase, namely rePBaxA, was purified to homogeneity by using Ni-affinity resin and its molecular weight was 35.0kDa. The optimum temperature and pH of rePBaxA were 50°C and 5.0, respectively. The kinetic parameters Michaelis-Menten constant (Km) and maximum reaction rate (Vmax) of rePBaxA were 5.41mg/mL and 22...
July 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28724263/mutagenesis-and-characterization-of-a-bacillus-amyloliquefaciens-strain-for-cinnamomum-camphora-seed-kernel-oil-extraction-by-aqueous-enzymatic-method
#5
Cheng Zeng, Rongbin Zhao, Maomao Ma, Zheling Zeng, Deming Gong
The purpose of the present study was to increase the proteinase activity of the strain NCU116 by combining ultraviolet irradiation and N-methyl-N'-nitro-N-nitroso guanidine treatment, in order to enhance the efficiency of Cinnamomum camphora seed kernel oil (CCSKO) extraction by aqueous enzymatic method (AEM). The mutated strain, designated as NCU116-1, was screened out by the ratio of hydrolytic zone diameter to colony diameter on skim milk plate. The proteinase activity (9116.1 U/ml) of NCU116-1 was increased by 31...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28719340/sequence-comparison-of-phor-gyrb-groel-and-chea-genes-as-phylogenetic-markers-for-distinguishing-bacillus-amyloliquefaciens-and-b-subtilis-and-for-identifying-bacillus-strain-b29
#6
C Yu, J Jin, L-Q Meng, H-H Xia, H-F Yuan, J Wang, D-S Yu, X-Y Zhao, C-Q Sha
Given the close genetic relationship between Bacillus amyloliquefaciens and B. subtilis, distinguishing the two solely based on their physiological and biochemical characteristics and 16S rRNA sequences is difficult. Molecular identification was used to discover suitable genes for distinguishing the two bacteria, and to identify the bio-controlling strain B29, due to molecular identification has been paid more and more attention. The similarity of four genes, cheA, gyrB, groEL and phoR, of the two species was compared by the software BLASTN and MAGA, and phylogenetic tree was constructed...
May 20, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28690159/probiotic-mediated-blueberry-vaccinium-corymbosum-l-fruit-fermentation-to-yield-functionalized-products-for-augmented-antibacterial-and-antioxidant-activity
#7
Byung-Taek Oh, Seong-Yeop Jeong, Palanivel Velmurugan, Jung-Hee Park, Do-Youn Jeong
The aim of this study was to investigate the fermentation of blueberry fruit with selected probiotic bacteria (Bacillus amyloliquefaciens and Lactobacillus brevis) and yeast (Starmerella bombicola) isolated from fermented starfish for the extraction of functionalized products for biomedical applications. All probiotic-based fermented extracts showed augmented antibacterial and antioxidant activity compared to the control. Biochemical parameters of viable cell count, titratable acidity, total phenol, total anthocyanin, total flavonoids, total sugar, and reducing sugar were analyzed during a 0-96 h fermentation period...
July 6, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28664219/biological-characterization-of-the-biocontrol-agent-bacillus-amyloliquefaciens-cpa-8-the-effect-of-temperature-ph-and-water-activity-on-growth-susceptibility-to-antibiotics-and-detection-of-enterotoxic-genes
#8
Amparo Gotor-Vila, Neus Teixidó, María Sisquella, Rosario Torres, Josep Usall
This work focuses on the biological understanding of the biocontrol agent Bacillus amyloliquefaciens CPA-8 in order to accomplish the characterization required in the registration process for the development of a microorganism-based product. The tolerance of CPA-8 to grow under different pH-temperature and water activity (a w)-temperature conditions was widely demonstrated. Regarding the pH results, optimum growth at the evaluated conditions was observed at 37 °C and pH between 7 and 5. On the contrary, the slowest growth was recorded at 20 °C and pH 4...
June 29, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28657581/bacillus-amyloliquefaciens-say09-increases-cadmium-resistance-in-plants-by-activation-of-auxin-mediated-signaling-pathways
#9
Cheng Zhou, Lin Zhu, Zhongyou Ma, Jianfei Wang
Without physical contact with plants, certain plant growth-promoting rhizobacteria (PGPR) can release volatile organic compounds (VOCs) to regulate nutrient acquisition and induce systemic immunity in plants. However, whether the PGPR-emitted VOCs can induce cadmium (Cd) tolerance of plants and the underlying mechanisms remain elusive. In this study, we probed the effects of Bacillus amyloliquefaciens (strain SAY09)-emitted VOCs on the growth of Arabidopsis plants under Cd stress. SAY09 exposure alleviates Cd toxicity in plants with increased auxin biosynthesis...
June 28, 2017: Genes
https://www.readbyqxmd.com/read/28647117/isolation-of-a-potential-biocontrol-agent-paenibacillus-polymyxa-nsy50-from-vinegar-waste-compost-and-its-induction-of-host-defense-responses-against-fusarium-wilt-of-cucumber
#10
Nanshan Du, Lu Shi, Yinghui Yuan, Jin Sun, Sheng Shu, Shirong Guo
Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (FOC) is one of the major destructive soil-borne diseases infecting cucumber. In this study, we screened 60 target strains isolated from vinegar waste compost, from which 10 antagonistic strains were identified to have the disease suppression capacity of bio-control agents. The 16S rDNA gene demonstrated that the biocontrol agents were Paenibacillus polymyxa (P. polymyxa), Bacillus amyloliquefaciens (B. amyloliquefaciens) and Bacillus licheniformis (B...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28613417/batch-batch-stable-microbial-community-in-the-traditional-fermentation-process-of-huyumei-broad-bean-pastes
#11
Linjiang Zhu, Zihao Fan, Hui Kuai, Qi Li
During natural fermentation processes, a characteristic microbial community structure (MCS) is naturally formed, and it is interesting to know about its batch-batch stability. This issue was explored in a traditional semi-solid-state fermentation process of huyumei, a Chinese broad bean paste product. The results showed that this MCS mainly contained four aerobic Bacillus species (8 Log cfu g(-1) ), including B. subtilis, B. amyloliquefaciens, B. methylotrophicus, and B. tequilensis, and the facultative anaerobe B...
June 14, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28600796/efficacy-of-bacillus-amyloliquefaciens-as-biocontrol-agent-to-fight-fungal-diseases-of-maize-under-tropical-climates-from-lab-to-field-assays-in-south-kivu
#12
Parent Zihalirwa Kulimushi, Géant Chuma Basime, Gustave Mushagalusa Nachigera, Philippe Thonart, Marc Ongena
In the province of South Kivu (Democratic Republic of Congo), warm and humid climatic conditions favor the development and spreading of phytopathogens. The resulting diseases cause important losses in production both in crop and after harvest. In this study, we wanted to evaluate the potential of Bacillus amyloliquefaciens as biocontrol agent to fight some newly isolated endemic fungal pathogens infesting maize. The strain S499 has been selected based on its high in vitro antagonistic activity correlating with a huge potential to secrete fungitoxic lipopeptides upon feeding on maize root exudates...
June 9, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28588570/the-profile-and-antimicrobial-activity-of-bacillus-lipopeptide-extracts-of-five-potential-biocontrol-strains
#13
Ivica Dimkić, Slaviša Stanković, Marija Nišavić, Marijana Petković, Petar Ristivojević, Djordje Fira, Tanja Berić
In this study the efficacy of two different methods for extracting lipopeptides produced by five Bacillus strains-ethyl acetate extraction, and acid precipitation followed by methanol extraction-was investigated using mass spectrometry. High performance thin layer chromatography (HPTLC) was also used for the simultaneous separation of complex mixtures of lipopeptide extracts and for the determination of antimicrobial activity of their components. The mass spectra clearly showed well-resolved groups of peaks corresponding to different lipopeptide families (kurstakins, iturins, surfactins, and fengycins)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28587617/construction-of-energy-conserving-sucrose-utilization-pathways-for-improving-poly-%C3%AE-glutamic-acid-production-in-bacillus-amyloliquefaciens
#14
Jun Feng, Yanyan Gu, Yufen Quan, Weixia Gao, Yulei Dang, Mingfeng Cao, Xiaoyun Lu, Yi Wang, Cunjiang Song, Shufang Wang
BACKGROUND: Sucrose is an naturally abundant and easily fermentable feedstock for various biochemical production processes. By now, several sucrose utilization pathways have been identified and characterized. Among them, the pathway consists of sucrose permease and sucrose phosphorylase is an energy-conserving sucrose utilization pathway because it consumes less ATP when comparing to other known pathways. Bacillus amyloliquefaciens NK-1 strain can use sucrose as the feedstock to produce poly-γ-glutamic acid (γ-PGA), a highly valuable biopolymer...
June 6, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28585422/menaquinone-7-production-from-maize-meal-hydrolysate-by-bacillus-isolates-with-diphenylamine-and-analogue-resistance
#15
Jian-Zhong Xu, Wei-Guo Zhang
A menaquinone-7 (MK-7) high-producing strain needs to be isolated to increase MK-7 production, in order to meet a requirement of MK-7 given the low MK-7 content in food products. This article focuses on developing MK-7 high-producing strains via screening and mutagenesis by an atmospheric and room temperature plasma (ARTP) mutation breeding system. We isolated an MK-7-producing strain Y-2 and identified it as Bacillus amyloliquefaciens, which produced (7.1±0.5) mg/L of MK-7 with maize meal hydrolysate as carbon source...
June 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28583081/addition-of-plant-growth-promoting-bacillus-subtilis-pts-394-on-tomato-rhizosphere-has-no-durable-impact-on-composition-of-root-microbiome
#16
Junqing Qiao, Xiang Yu, Xuejie Liang, Yongfeng Liu, Rainer Borriss, Youzhou Liu
BACKGROUND: Representatives of the genus Bacillus are increasingly used in agriculture to promote plant growth and to protect against plant pathogens. Unfortunately, hitherto the impact of Bacillus inoculants on the indigenous plant microbiota has been investigated exclusively for the species Bacillus amyloliquefaciens and was limited to prokaryotes, whilst eukaryotic member of this community, e.g. fungi, were not considered. RESULTS: The root-colonizing Bacillus subtilis PTS-394 supported growth of tomato plants and suppressed soil-borne diseases...
June 5, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28578227/evaluating-the-feasibility-of-fermentation-starter-inoculated-with-bacillus-amyloliquefaciens-for-improving-acetoin-and-tetramethylpyrazine-in-baoning-bran-vinegar
#17
Liqiang Zhang, Jun Huang, Rongqing Zhou, Chongde Wu
Fermentation starters (Daqu) used in present study included traditional herb Daqu (C Daqu), modified Daqu without herbs (M Daqu) and S Daqu fermented by inoculating acetoin and tetramethylpyrazine high-producing bacterium Bacillus amyloliquefaciens into M Daqu. To evaluate the feasibility of S Daqu combined with M Daqu applied for improving contents of acetoin and tetramethylpyrazine in Baoning bran vinegar without remarkably changing the original microbial community and the other volatiles contents compared with C Daqu, vinegar Pei C, M, M1, M2 and S were correspondingly prepared in lab scale using C Daqu, M Daqu, M1 Daqu (S Daqu: M Daqu=1:9, w/w), M2 Daqu (S Daqu: M Daqu=5:5) and S Daqu...
May 27, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28577516/improvement-of-bacillus-strains-by-mutation-for-overproduction-of-exopolygalacturonases
#18
Hira Muzzamal, Zakia Latif
Pectinases, produced by microorganisms, have wide range application in food industry, textile processing, paper making, coffee and tea fermentation, etc. It accounts for 10% of the global industrial enzymes produced. The most important and widely used commercial pectinase polygalacturonase is produced by alkalophilic strains of Bacillus sp. and Streptomyces sp. Here, we explored 29 bacterial strains isolated from rotten mango samples for polygalacturonase production and selected 16 strains through preliminary screening by well-plate method for enzyme activity...
August 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/28574306/impact-of-mannanase-producing-bacillus-spp-on-the-accuracy-of-the-3m-petrifilm-aerobic-count-method
#19
Guoping Feng, Amanda Hew, Ramesh Manoharan, Siva Subramanian
Consistent deviations of the 3M Petrifilm aerobic counts (AC) from the standard pour plate aerobic plate count (APC) were observed with dehydrated onion and garlic products. A large study was designed to determine the relationship of these two methods and the root cause for the deviations. A total of 3,800 dehydrated onion and garlic samples were analyzed by both the Petrifilm AC and the standard pour plate APC method. Large spreader-like liquefied areas were observed on numerous Petrifilm plates. These liquefied areas made enumeration inaccurate...
June 2, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28571307/variants-of-lipopeptides-and-glycolipids-produced-by-bacillus-amyloliquefaciens-and-pseudomonas-aeruginosa-cultured-in-different-carbon-substrates
#20
Thando Ndlovu, Marina Rautenbach, Sehaam Khan, Wesaal Khan
The quantitative and qualitative effect of water immiscible and miscible carbon-rich substrates on the production of biosurfactants, surfactin and rhamnolipids, by Bacillus amyloliquefaciens ST34 and Pseudomonas aeruginosa ST5, respectively, was analysed. A small-scale high throughput 96 deep-well micro-culture method was utilised to cultivate the two strains in mineral salt medium (MSM) supplemented with the water miscible (glucose, glycerol, fructose and sucrose) and water immiscible carbon sources (diesel, kerosene and sunflower oil) under the same growth conditions...
December 2017: AMB Express
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