Menglin She, Huijuan Zhou, Wanrong Dong, Yuxiang Xu, Lin Gao, Jiaming Gao, Yong Yang, Zhifan Yang, Dongbo Cai, Shouwen Chen
As a kind of biosurfactants, iturin A has attracted people's wide attentions due to their features of biodegradability, environmentally friendly, etc.; however, high production cost limited its extensive application, and the aim of this research wants to improve iturin A production in Bacillus amyloliquefaciens. Firstly, dual promoter was applied to strengthen iturin A synthetase expression, and its yield was increased to 1.25 g/L. Subsequently, original 5'-UTRs of downstream genes (ituA, ituB, and ituC) in iturin A synthetase cluster were optimized, which significantly increased mRNA secondary stability, and iturin A yield produced by resultant strain HZ-T3 reached 2...
April 27, 2024: Applied Microbiology and Biotechnology