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Bacillus amyloliquefaciens

Tie-Lin Wang, Dong-Yue Zhao, Mei-Lan Chen, Zhong-Hua Ma, Lan-Ping Guo
A rhizobacteria strain named RS-3 exhibited inhibitory activity against all five Panax ginseng pathogens was isolated from the root of P. ginseng. This strain was identified as Bacillus amyloliquefaciens based on its morphological character and 16S rDNA sequence. Antagonistic activity experiments indicated that the strain could strongly suppress Botrytis cinerea Pers with an inhibitory rate of 54.4%, suggesting the potentialities of biocontrol agent against diseases that frequently happen on ginseng.
December 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Fengying Dong, Lingmeng Li, Lin Lin, Dannong He, Jingwen Chen, Wei Wei, Dongzhi Wei
This work presents a synthetic route to produce chloramphenicol esters by taking advantage the high enantio- and regio-selectivity of lipases. A series of chloramphenicol esters were synthesized using chloramphenicol, acyl donors of different carbon chain length and lipase LipBA (lipase cloned from Bacillus amyloliquefaciens). Among acyl donors with different carbon chain lengths, vinyl propionate was found to be the best. The influences of different organic solvents, reaction temperature, reaction time, enzyme loading and water content on the synthesis of the chloramphenicol esters were studied...
September 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Qing-Xia Zhang, Ying Zhang, Hai-Huan Shan, Yun-Hui Tong, Xi-Jun Chen, Feng-Quan Liu
Antifungal metabolites produced by Bacillus sp. W10, which was previously isolated from the tomato rhizosphere, were investigated. Strain W10 was identified as Bacillus amyloliquefaciens by analysis of its 16S rDNA and gyrB gene partial sequences. PCR analysis showed the presence of fenB, sfp, and ituD genes, coding for fengycin, surfactin, and iturin, respectively. A novel small antifungal peptide, designated 5240, produced by this strain was isolated by ammonium sulfate precipitation and Superdex 200 gel filtration chromatography...
September 17, 2017: Environmental Science and Pollution Research International
Yongbin Xu, Inseong Jo, Lulu Wang, Jinli Chen, Shengdi Fan, Yuesheng Dong, Chun-Shan Quan, Nam-Chul Ha
Membrane fusion proteins (MFPs) play an essential role in the action of the drug efflux pumps and protein secretion systems in bacteria. The sporulation delaying protein (SDP) efflux pump YknWXYZ has been identified in diverse Bacillus species. The MFP YknX requires the ATP-binding cassette (ABC) transporter YknYZ and the Yip1 family protein YknW to form a functional complex. To date, the crystal structure, molecular function and mechanism of action of YknX remain unknown. In this study, to characterize the structural and biochemical roles of YknX in the functional assembly of YknWXYZ from B...
September 13, 2017: Biochemical and Biophysical Research Communications
Shaofang Liu, Haiting Hao, Xiang Lu, Xia Zhao, Yun Wang, Yubao Zhang, Zhongkui Xie, Ruoyu Wang
Plant growth-promoting Bacillus amyloliquefaciens FZB42 induces systemic salt tolerance in Arabidopsis and enhances the fresh and dry weight. However, the underlying molecular mechanism that allows plants to respond to FZB42 and exhibit salt tolerance is largely unknown. Therefore, we performed large-scale transcriptome sequencing of Arabidopsis shoot tissues grown under salt stress with or without FZB42 inoculation by using Illumina sequencing to identify the key genes and pathways with important roles during this interaction...
September 13, 2017: Scientific Reports
Shalini Tiwari, Vivek Prasad, Puneet S Chauhan, Charu Lata
Being sessile in nature, plants have to withstand various adverse environmental stress conditions including both biotic and abiotic stresses. Comparatively, abiotic stresses such as drought, salinity, high temperature, and cold pose major threat to agriculture by negatively impacting plant growth and yield worldwide. Rice is one of the most widely consumed staple cereals across the globe, the production and productivity of which is also severely affected by different abiotic stresses. Therefore, several crop improvement programs are directed toward developing stress tolerant rice cultivars either through marker assisted breeding or transgenic technology...
2017: Frontiers in Plant Science
Juan Yang, Dongxiao Sun-Waterhouse, Chun Cui, Keming Dong, Wei Wang
The transpeptidase activity of glutaminase from Bacillus amyloliquefaciens (GBA) and Aspergillus oryzae (GAO) to yield γ-[Glu]n-Phe peptides were verified for the first time. In the presence of Gln and Phe, γ-Glu-Phe and γ-Glu-γ-Glu-Phe were synthesized by GAO, and γ-Glu-Phe, γ-Glu-γ-Glu-Phe, γ-Glu-γ-Glu-γ-Glu-Phe, γ-Glu-γ-Glu-γ-Glu-γ-Glu-Phe and γ-Glu-γ-Glu-γ-Glu-γ-Glu-γ-Glu-Phe were synthesized by GBA. The Km values for the transpeptidation catalysed by GBA and GAO were 47.88 and 153.92 mM (Phe as the acceptor), 84...
September 13, 2017: Journal of Agricultural and Food Chemistry
Yihwan Kim, InSong Koh, Mi Young Lim, Won-Hyong Chung, Mina Rho
Recent advances in high-throughput sequencing technology allow for in-depth studies on microbial genomes and their communities. While multiple strains of the same species could display genomic variations with different gene contents in diverse habitats and hosts, the essential functions for a specific species are conserved as core genes that are shared among strains. We have comprehensively analyzed 238 strains of five different Bacillus species to identify the properties of core and strain-specific genes. Core and strain-specific genes in each Bacillus species show significant differences in their functions and genomic signatures...
September 8, 2017: Scientific Reports
Min-Ji Kim, Ramalingam Radhakrishnan, Sang-Mo Kang, Young-Hyun You, Eun-Ju Jeong, Jong-Guk Kim, In-Jung Lee
This study was aimed to identify plant growth-promoting bacterial isolates from soil samples and to investigate their ability to improve plant growth and salt tolerance by analysing phytohormones production and phosphate solubilisation. Among the four tested bacterial isolates (I-2-1, H-1-4, H-2-3, and H-2-5), H-2-5 was able to enhance the growth of Chinese cabbage, radish, tomato, and mustard plants. The isolated bacterium H-2-5 was identified as Bacillus amyloliquefaciens H-2-5 based on 16S rDNA sequence and phylogenetic analysis...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
Md Saifur Rahman, Yun Hee Choi, Yoon Seok Choi, Md Badrul Alam, Sang Han Lee, Jin Cheol Yoo
A novel antioxidant peptide YD1 (∼1.0kDa), purified from the strain Bacillus amyloliquefaciens CBSYD1, displayed activity in several in vitro assays and was also efficient against Gram-positive, Gram-negative as well as multidrug-resistant (MDR) bacteria. Strain growth was adapted to bile-salt conditions where a clear halos-zone was observed in a bile-salt plate assay and was viable in different digestive track conditions. YD1 treatment on RAW 264.7 cells increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) through the enhanced levels of heme oxygenase-1 (HO-1)...
January 15, 2018: Food Chemistry
Samah A El-Newary, Abeer Y Ibrahim, Mohsen S Asker, Manal G Mahmoud, Mohamed E El Awady
OBJECTIVE: To evaluate in-vitro antioxidant, anti-inflammatory and antitumor abilities against human breast adenocarcinoma (MCF7) and human prostate cancer (PC3) as well as the suppressor effect of bacterial exopolysaccharide (BAEPS) on Ehrlich ascites carcinoma (EAC). METHODS: In-vitro antioxidants characters of BAEPS were determined using various methods, while anti-inflammatory activity was estimated against cyclooxygenase (COX-1 and COX-2). In-vitro study, anticancer against MCF7 and PC3 were assessed by the mitochondrial dependent reduction of yellow MTT...
July 2017: Asian Pacific Journal of Tropical Medicine
Olfa Frikha-Gargouri, Dorra Ben Abdallah, Ilhem Bhar, Slim Tounsi
This study aimed to improve the screening method for the selection of Bacillus biocontrol agents against crown gall disease. The relationship between the strain biocontrol ability and their in vitro studied traits was investigated to identify the most important factors to be considered for the selection of effective biocontrol agents. In fact, previous selection procedure relying only on in vitro antibacterial activity was shown to be not suitable in some cases. A direct plant-protection strategy was performed to screen the 32 Bacillus biocontrol agent candidates...
2017: Frontiers in Plant Science
Laura Vilanova, Neus Teixidó, Josep Usall, Marta Balsells-Llauradó, Amparo Gotor-Vila, Rosario Torres
BACKGROUND: Bacillus amyloliquefaciens strain CPA-8 has been described as an effective biocontrol agent to control brown rot in stone fruit for both preharvest and postharvest applications. However, no information about the environmental fate and behaviour of this strain under field conditions is available. RESULTS: The dispersion of the CPA-8 application was evaluated using water-sensitive papers, and complete coverage was observed on the leaves of treated trees, while less than 1 % of non-treated tree leaves had CPA-8...
August 28, 2017: Pest Management Science
Francisco Salazar, Aurelio Ortiz, Estibaliz Sansinenea
OBJECTIVES: The aim of this study was the isolation and characterizationof antifungal and bactericidal compounds from B. amyloliquefaciens strain ELI149. METHODS: For isolation and purification purposes, absorbent resin calledamberlite XAD-16 and silica gel column chromatography were used, respectively. Antibacterial and antifungal assays by well-diffusion method were performed to demonstrate the biological activity of each compound. The cell damage on the tested fungi was evaluated for fengycin under phase contrast microscopy...
August 24, 2017: Journal of Global Antimicrobial Resistance
Kajal Chakraborty, Bini Thilakan, Vamshi Krishna Raola
A heterotrophic marine bacterium Bacillus amyloliquefaciens isolated from seaweed Padina gymnospora exhibited broad spectra of antibacterial activities against pathogenic bacteria Aeromonas hydrophila, Vibrio harveyi, Vibrio vulnificus, and Vibrio parahaemolyticus. The seaweed-associated B. amyloliquefaciens was recognized to possess functional type I polyketide synthase-1 (pks-1) gene, and was used to isolate four homologous compounds with polyketide frameworks. The compounds were characterized as 11-(15-butyl-13-ethyl-tetrahydro-12-oxo-2H-pyran-13-yl) propyl-2-methylbenzoate (1), 9-(tetrahydro-12-isopropyl-11-oxofuran-10-yl)-ethyl-4-ethoxy-2-hydroxybenzoate (2), 12-(aminomethyl)-11-hydroxyhexanyl-10-phenylpropanoate (3), and 7-(14-hydroxypropan-13-yl)-8-isobutyl-7,8-dihydrobenzo[c]oxepin-1(3H)-one (4) by comprehensive nuclear magnetic resonance and mass spectroscopic experiments...
August 25, 2017: Applied Biochemistry and Biotechnology
Yuling Liao, Yanrui Ye, Bin Wang, Li Pan
OBJECTIVES: To deregulate the purine operon of the purine biosynthetic pathway and optimize energy generation of the respiratory chain to improve the yield of guanosine in Bacillus amyloliquefaciens XH7. RESULTS: The 5'-untranslated region of the purine operon, which contains the guanine-sensing riboswitch, was disrupted. The native promoter Pw in B. amyloliquefaciens XH7 was replaced by different strong promoters. Among the promoter replacement mutants, XH7purE::P41 gave the highest guanosine yield (16...
August 24, 2017: Biotechnology Letters
Jun Yuan, Mengli Zhao, Rong Li, Qiwei Huang, Waseem Raza, Christopher Rensing, Qirong Shen
Volatile organic compounds (VOCs) from soil bacteria are likely to have an important role in the interactions among soil microorganisms. However, their effects on the soil microbial community have not been extensively studied. In this study, the effect of bacterial VOCs generated by growing Bacillus amyloliquefaciens NJN-6 on modified MS medium on soil microbial community was evaluated. B. amyloliquefaciens NJN-6 was able to produce 48 volatile compounds as determined by solid-phase microextraction-GC/MS. MiSeq sequencing data showed that bacterial VOCs could alter the composition of both soil bacterial and soil fungal communities and could decrease the alpha-diversity of the soil microbial community...
August 12, 2017: Environmental Science and Pollution Research International
M C Moura, D S Trentin, T H Napoleão, M Primon-Barros, A S Xavier, N P Carneiro, P M G Paiva, A J Macedo, L C B B Coelho
AIMS: To evaluate the antibiofilm potential of water-soluble Moringa oleifera seed lectin (WSMoL) on Serratia marcescens and Bacillus sp. METHODS AND RESULTS: WSMoL inhibited biofilm formation by S. marcescens at concentrations lower than 2·6 μg ml(-1) and impaired bacterial growth at higher concentrations, avoiding biofilm formation. For Bacillus sp., the lectin inhibited bacterial growth at all concentrations. The antibiofilm action of WSMoL is associated with damage to bacterial cells...
August 9, 2017: Journal of Applied Microbiology
An Lee, Kuan-Chen Cheng, Je-Ruei Liu
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by Fusarium species, which has been shown to be associated with reproductive disorders in livestock, and to a lesser extent with hyperoestrogenic syndromes in humans. The aim of this study was to characterize a Bacillus amyloliquefaciens strain with ZEN removal ability. A pure culture of a strain designated LN isolated from moldy corn samples showed a high ZEN removal capability. Based on microscopic observations, biochemical characteristics, and phylogenetic analysis of the 16S rRNA gene sequence, LN was identified as B...
2017: PloS One
Cai Hui, Xiaoxiao Guo, Pengfei Sun, Rashid Azim Khan, Qichun Zhang, Yongchao Liang, Yu-Hua Zhao
A newly verified adsorbent biofilm produced by Bacillus amyloliquefaciens DT was investigated for nitrite removal from aqueous solutions. The biofilm's characteristics and adsorption mechanism were determined, with results indicating that nitrite ions were adsorbed onto the protonated amine sites of biofilm under acidic conditions. Analysis of various factors showed that higher nitrite adsorption capacities occurred at pH < 3.0 and higher temperatures as well as higher initial nitrite concentrations, with a maximum nitrite removal capacity of 116...
July 1, 2017: Bioresource Technology
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