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Edson Costa E Silva, Sheyla Viana Omonte, Alessandro Gomides Veiga Martins, Hércules Henrique Onibene de Castro, Hayder Egg Gomes, Élton Gonçalves Zenóbio, Peterson Antônio Dutra de Oliveira, Martinho Campolina Rebello Horta, Paulo Eduardo Alencar Souza
OBJECTIVE: The aim of this study was to evaluate the effect of hyaluronic acid (HA) in the structure and degradation patterns of BioGide(®) and OsseoGuard™ collagen membranes. HA mediates inflammation and acts in cell migration, adhesion, and differentiation, benefitting tissue remodeling and vascularization. These are desirable effects in guided regeneration procedures, but it is still unknown whether HA alters the barrier properties of absorbable membranes. DESIGN: Bone defects were created in the calvaria of rats, which were treated with HA gel 1% (HA group) or simply filled with blood clot (control group), and covered with BioGide(®) or OsseoGuard™...
October 17, 2016: Archives of Oral Biology
Zahra Aghili, Saba Taheri, Hojjat Alizadeh Zeinabad, Leila Pishkar, Ali Akbar Saboury, Arash Rahimi, Mojtaba Falahati
Herein, the interaction of hen egg white lysozyme (HEWL) with iron nanoparticle (Fe NP) was investigated by spectroscopic and docking studies. The zeta potential analysis revealed that addition of Fe NP (6.45±1.03 mV) to HEWL (8.57±0.54 mV) can cause to greater charge distribution of nanoparticle-protein system (17.33±1.84 mV). In addition, dynamic light scattering (DLS) study revealed that addition of Fe NP (92.95±6.11 nm) to HEWL (2.68±0.37 nm) increases suspension potential of protein/nanoparticle system (51...
2016: PloS One
Parthiban Subramanian, In-Chan Choi, Vimalraj Mani, Junhyung Park, Sathiyamoorthy Subramaniyam, Kang-Hyun Choi, Joon-Soo Sim, Chang-Muk Lee, Ja Choon Koo, Bum-Soo Hahn
In this study, we investigated global changes in miRNAs of Meloidogyne incognita throughout its life cycle. Small RNA sequencing resulted in approximately 62, 38, 38, 35, and 39 Mb reads in the egg, J2, J3, J4, and female stages, respectively. Overall, we identified 2724 known and 383 novel miRNAs (read count > 10) from all stages, of which 169 known and 13 novel miRNA were common to all the five stages. Among the stage-specific miRNAs, miR-286 was highly expressed in eggs, miR-2401 in J2, miR-8 and miR-187 in J3, miR-6736 in J4, and miR-17 in the female stages...
October 21, 2016: International Journal of Molecular Sciences
Mauro Zaninelli, Veronica Redaelli, Erica Tirloni, Cristian Bernardi, Vittorio Dell'Orto, Giovanni Savoini
The development of a monitoring system to identify the presence of laying hens, in a closed room of a free-range commercial organic egg production farm, was the aim of this study. This monitoring system was based on the infrared (IR) technology and had, as final target, a possible reduction of atmospheric ammonia levels and bacterial load. Tests were carried out for three weeks and involved 7 ISA (Institut de Sélection Animale) brown laying hens. The first 5 days was used to set up the detection sensor, while the other 15 days were used to evaluate the accuracy of the resulting monitoring system, in terms of sensitivity and specificity...
October 21, 2016: Sensors
Suzanne Faxneld, Urs Berger, Björn Helander, Sara Danielsson, Anna Aroha Miller, Elisabeth Nyberg, Jan-Olov Persson, Anders Bignert
Temporal and spatial trends of perfluoroalkyl acids (PFAAs) were investigated in Baltic Sea herring liver (Clupea harengus) from three sites, and white-tailed sea eagle (WTSE) eggs (Haliaeetus albicilla) from two freshwater and two marine areas in Sweden. Trends of most quantifiable PFAAs increased over the monitored period (1980 - 2014 in herring, 1960s/1980s - 2010 in WTSE). No significant decreasing trends were observed for the most recent ten years for any substances, except perfluorooctane sulfonamide (FOSA)...
October 24, 2016: Environmental Science & Technology
Margaret K Nelson, Rosanna Hertz, Wendy Kramer
This paper discusses the attitudes of three groups of stakeholders in the world of assisted reproduction gamete donors, parents who use donated gamete, and offspring conceived with donated gametes with respect to the two issues of donor anonymity and limits on the number of offspring a single donor can produce. The data are drawn from on-line surveys which were made available between May 12, 2104 and August 15, 2014 to gamete donors, donor-conceived offspring, and parents who used donated gametes to conceive...
April 2016: Journal of Law and the Biosciences
Asuka Onuma, Wataru Fujii, Koji Sugiura, Kunihiko Naito
Genome editing using the CRISPR/Cas system can induce mutations with high efficiency, and allows easier production of genome-modified animals than that offered by the conventional method where embryonic stem cells are used. However, studies using CRISPR/Cas systems have been mostly limited to proliferating somatic cells and pronuclear-stage fertilized eggs. In contrast, the efficiency of a CRISPR/Cas system in immature and maturing oocytes progressing through meiosis has not yet been assessed. In the present study, we evaluated the genome-modification efficiency of the CRISPR/Cas system during meiotic maturation of porcine oocytes...
October 21, 2016: Journal of Reproduction and Development
P Glynn Tillman, Ted E Cottrell
Chinavia hilaris (Say), Euschistus servus (Say), Euschistus tristigmus (Say), and Thyanta custator custator (F.) (Hemiptera: Pentatomidae) are serious pests of crops in the southeastern United States but little is known concerning their dispersal from noncrop hosts in woodlands into crops. This 2-yr study was conducted to investigate whether elderberry [Sambucus nigra subsp. canadensis (L.) R. Bolli] in woodlands serves as a source of stink bugs dispersing into adjacent crops and to examine parasitism of C...
2016: Journal of Insect Science
Alexandre Webster, Melina Schuh
Eggs and sperm develop through a specialized cell division called meiosis. During meiosis, the number of chromosomes is reduced by two sequential divisions in preparation for fertilization. In human female meiosis, chromosomes frequently segregate incorrectly, resulting in eggs with an abnormal number of chromosomes. When fertilized, these eggs give rise to aneuploid embryos that usually fail to develop. As women become older, errors in meiosis occur more frequently, resulting in increased risks of infertility, miscarriage, and congenital syndromes, such as Down's syndrome...
September 27, 2016: Trends in Cell Biology
Mitiku Bajiro, Daniel Dana, Mio Ayana, Daniel Emana, Zeleke Mekonnen, Belay Zawdie, Asfaw Garbi, Ashenafi Kure, Ahmed Zeynudin
BACKGROUND: Intestinal schistosomiasis is one of the neglected tropical parasitic diseases caused by Schistosoma mansoni. Currently, the control measures for the disease are mainly based on mass drug administration (MDA) with praziquantel (PZQ) targeting the school-age children. In Ethiopia, the potential foci for schistosomiasis and therapeutic efficacy of PZQ among school-age children remain poorly explored. Therefore, we determined both the prevalence and intensity of S. mansoni infection and the therapeutic efficacy of PZQ among school children in the Manna District (new foci for S...
October 24, 2016: Parasites & Vectors
Steffen Rehbein, Martin Knaus, Yasmina Mallouk, Tatjana Breiltgens, Emanuele Brianti, Balázs Capári, Filipe Dantas-Torres, Michel Gau, Anja Joachim, Karl-Heinz Kaulfuß, Zvezdelina Kirkova, Joerg Lechner, Andrei D Mihalca, Rosamaria Mirabito, Saulius Petkevičius, Dhimitër Rapti, Enstela Shukullari, Michel Sedeilhan, Doris Dollhofer, Katrin Kley, Wilfried Lebon, Martin Visser, Philippe Jeannin
Afoxolaner (AFX) plus milbemycin oxime (MO) combination chewable tablets (NexGard Spectra®, Merial) were evaluated for safety and efficacy against naturally acquired nematode infections in domestic dogs in a multi-centre, positive control, blinded field study using a randomized block design based on the order of presentation for allocation. In total, 408 dogs confirmed positive for naturally acquired infections of intestinal nematodes by pre-treatment faecal examination were studied in ten countries in Europe (Albania, Austria, Bulgaria, France, Germany, Hungary, Italy, Lithuania, Romania and Slovakia)...
October 22, 2016: Parasitology Research
Pamela Mariana Burella, Maria Fernanda Simoniello, Gisela Laura Poletta
The agricultural expansion over the past decades, along with the associated increase in the use of pesticides, represents a high risk for many wild species. Caiman latirostris is a South American caiman with many features that make it highly vulnerable to pesticide exposure. Considering previous finding on the genotoxicity of the glyphosate-based formulation Roundup(®) in this species, the aim of this study was to evaluate the possible stage-dependent effect of this compound on C. latirostris embryos through the Comet assay (CA), micronuclei (MN), and nuclear abnormalities (NA) tests...
October 22, 2016: Archives of Environmental Contamination and Toxicology
Qixiao Jiang, Chunbo Wang, Chan Xue, Lingfang Xue, Meiting Wang, Changhao Li, Ziwen Deng, Qian Wang
Perfluorooctanoic acid (PFOA), a persistent organic pollutant, is associated with developmental toxicity. This study investigated the mechanism of PFOA-induced developmental cardiotoxicity in chicken embryo, focusing on the interactions between developmental exposure to PFOA and the levels of l-carnitine (LC), acetyl-l-carnitine (ALC) and propionyl-l-carnitine (PLC) in the heart. To evaluate the developmental cardiotoxicity, fertile chicken eggs were exposed to 0.1, 0.5, 1, 2 or 5mg/kg PFOA via air cell injection...
October 19, 2016: Environmental Toxicology and Pharmacology
Susanne E Pors, Ida J Pedersen, Ragnhild Bager Skjerning, Ida C N Thøfner, Gry Persson, Anders M Bojesen
Gallibacterium anatis causes infections in the reproductive tract of egg-laying hens and induce increased mortality and decreased egg production. New prophylactic measures are needed in order to improve animal welfare and production efficiency. Bacterial outer membrane vesicles (OMVs) have previously shown promising results in protection against infections and we hypothesized that OMVs could serve as an immunogen to protect egg-laying hens against G. anatis. To investigate the immunogenic potential of G. anatis OMVs, two in vivo studies in egg-laying hens were made...
November 15, 2016: Veterinary Microbiology
Espen Mariussen, Lene Sørlie Heier, Hans Christian Teien, Marit Nandrup Pettersen, Tor Fredrik Holth, Brit Salbu, Bjørn Olav Rosseland
An environmental survey was performed in Lake Kyrtjønn, a small lake within an abandoned shooting range in the south of Norway. In Lake Kyrtjønn the total water concentrations of Pb (14µg/L), Cu (6.1µg/L) and Sb (1.3µg/L) were elevated compared to the nearby reference Lake Stitjønn, where the total concentrations of Pb, Cu and Sb were 0.76, 1.8 and 0.12µg/L, respectively. Brown trout (Salmo trutta) from Lake Kyrtjønn had very high levels of Pb in bone (104mg/kg w.w.), kidney (161mg/kg w.w.) and the gills (137mg/kg d...
October 19, 2016: Ecotoxicology and Environmental Safety
Anna Hegsted, Forrest A Wright, SarahBeth Votra, David Pruyne
Formins are regulators of actin filament dynamics. We demonstrate here that two formins, FHOD-1 and EXC-6, are important in the nematode Caenorhabditis elegans for ovulation, during which actomyosin contractions push a maturing oocyte from the gonad arm into a distensible bag-like organ, the spermatheca. EXC-6, a homolog of the disease-associated mammalian formin INF2, is highly expressed in the spermatheca, where it localizes to cell-cell junctions and to circumferential actin filament bundles. Loss of EXC-6 does not noticeably affect the organization the actin filament bundles, and causes only a very modest increase in the population of junction-associated actin filaments...
October 22, 2016: Cytoskeleton
Panat Anuracpreeda, Amaya Watthanadirek, Runglawan Chawengkirttikul, Prasert Sobhon
A number of monoclonal antibodies (MoAbs) against the 16 kDa antigen of Paramphistomum gracile (16 kDaAgPg) were produced in vitro by hybridoma technique. Reactivity and specificity of these MoAbs were evaluated by ELISA and immunoblotting assays. Seven MoAb clones were selected from the stable hybridoma clones, namely 1D10, 2D7, 3B10, 3D9, 4F1, 4G4, and 5G12. It was found to be IgM and kappa light chain isotypes. By immunoblotting and ELISA, all MoAbs reacted with purified 16 kDaAgPg at molecular weight (MW) of 16 kDa and with the native 16 kDa antigen at MW of 16 kDa in the whole body (WB) and excretory-secretory (ES) fractions, but not with tegumental antigens (TA) of adult fluke...
October 22, 2016: Parasitology Research
M S Tan, E J Teoh, C P Hor, A A C Yeoh
INTRODUCTION: Children who develop any hypersensitivity reaction to eggs are routinely referred to hospital for Measles-Mumps-Rubella (MMR) vaccination as inpatients to prevent anaphylaxis. We aimed to study the association between hypersensitivity reactions after egg exposure and similar reactions after MMR immunisation; and examine the necessity of hospital admission for vaccination. METHODS: A prospective observational study was conducted in Paediatric Department in Bukit Mertajam Hospital, Penang, between March and December 2014...
August 2016: Medical Journal of Malaysia
Huilin Ou, Wei Yao, Nanping Wu, Frederick X C Wang, Tianhao Weng, Chengcong Han, Xiangyun Lu, Dongshan Yu, Haibo Wu, Linfang Cheng, Honglin Chen, Hangping Yao, Lanjuan Li
Developing a safe and effective H7N9 influenza vaccine was initiated in early spring 2013, following human infections with a novel avian influenza A (H7N9) virus. In this study, a candidate H7N9 vaccine seed strain is produced using reverse genetics, with HA and NA derived from a human H7N9 virus and the remaining genes from the PR8 backbone virus which grows well in eggs. We verified that the virulence and transmissibility of the recombinant H7N9 vaccine seed strain were decreased as compared to wild-type H7N9 virus, to levels comparable with PR8...
October 19, 2016: Oncotarget
Yingying Hong, Kyle Schmidt, Danielle Marks, Samantha Hatter, Anne Marshall, Luiz Albino, Paul Ebner
Numerous studies have assessed the efficacy of phage-based methods to inhibit Salmonella contamination in food products. As with most antibacterials, bacteria can develop resistance to phage in vitro. Here, we applied a single broad-spectrum Salmonella phage, vB_SalS_SJ_2 (SJ2; 10(8) PFU; MOI = 10), to Salmonella-contaminated meat and eggs to quantify the development of resistance in actual food matrices. Treatment with a single phage significantly reduced Salmonella Typhimurium contamination in both ground pork and liquid egg at various time points...
October 21, 2016: Foodborne Pathogens and Disease
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