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Liquid Chromatography tandem mass spectrometry

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https://www.readbyqxmd.com/read/29331795/development-and-validation-of-an-uhplc-ms-ms-approach-for-simultaneous-quantification-of-five-bioactive-saponins-in-rat-plasma-application-to-a-comparative-pharmacokinetic-study-of-aqueous-extracts-of-raw-and-salt-processed-achyranthes-bidentata
#1
Yi Tao, Yingshan Du, Weidong Li, Baochang Cai
A simple, accurate and sensitive ultra high-performance liquid chromatography-tandem mass spectrometry approach was established for the simultaneous determination of β-ecdysterone, 25S-inokosterone, ginsenoside Ro, chikusetsusaponin IV and chikusetsusaponin IVa in rat plasma after oral administration of raw and salt-processed Achyranthes bidentata extract. The saponins were completely separated on a Waters BEH C18 UHPLC column by using acetonitrile/0.1% formic acid-water as mobile phases. The mass analysis was performed in a triple quadrupole mass spectrometer using multiple reaction monitoring (MRM) with negative scan mode...
January 9, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29331742/a-sensitive-and-selective-immunoaffinity-column-clean-up-coupled-to-uplc-ms-ms-for-determination-of-trace-methyl-3-quinoxaline-2-carboxylic-acid-in-animal-tissues
#2
Peipei Li, Xiaojun Zhang, Juanmei Zhang, Zhongyong Yan, Shuai Zhang, Si Chen, Yi Fang
This paper described a reliable and simple method for the selective determination of MQCA in animal tissues using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A highly targeted immunoaffinity column was used for sample purification after enzymatic hydrolysis. The purified extracts were analyzed by reversed-phase HPLC-MS/MS in positive ESI and multiple reaction monitoring mode. The calibration curves showed good linearity with correlation coefficient (r2) larger than 0...
January 3, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29331073/cerebrospinal-fluid-macrophage-biomarkers-in-amyotrophic-lateral-sclerosis
#3
A G Thompson, E Gray, M-L Thézénas, P D Charles, S Evetts, M T Hu, K Talbot, R Fischer, B M Kessler, M R Turner
Objective The neurodegenerative disease amyotrophic lateral sclerosis (ALS) is a heterogeneous clinical syndrome involving multiple molecular pathways. The development of biomarkers for use in therapeutic trials is a priority. We sought to use a high-throughput proteomic method to identify novel biomarkers in individual cerebrospinal fluid samples. Methods Liquid chromatography-tandem mass spectrometry with label-free quantification was used to identify cerebrospinal fluid proteins using samples from a well-characterised longitudinal cohort comprising patients with ALS (n=43), the upper motor neuron variant primary lateral sclerosis (PLS, n=6), cross-sectional healthy (n=20) and disease controls (Parkinsons's n=20, ALS mimic disorders n=12)...
January 13, 2018: Annals of Neurology
https://www.readbyqxmd.com/read/29330793/hypoxia-induced-metabolomic-alterations-in-pancreatic-cancer-cells
#4
Venugopal Gunda, Sushil Kumar, Aneesha Dasgupta, Pankaj K Singh
Hypoxic conditions in the pancreatic tumor microenvironment lead to the stabilization of hypoxia-inducible factor-1 alpha (HIF-1α), which acts as the master regulator of cancer cell metabolism. HIF-1α-mediated metabolic reprogramming results in large-scale metabolite perturbations. Characterization of the metabolic intermediates and the corresponding metabolic pathways altered by HIF-1α would facilitate the identification of therapeutic targets for hypoxic microenvironments prevalent in pancreatic ductal adenocarcinoma and other solid tumors...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29329874/a-survey-of-free-glutamic-acid-in-foods-using-a-robust-lc-ms-ms-method
#5
Nur Cebi, Canan Ekinci Dogan, Elmas Oktem Olgun, Osman Sagdic
An effective and simultaneous liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used with the aim of quantifying monosodium glutamate (MSG) in foodstuffs, such as chips, taste cubes, sauces and soups. The results were linear (R2 = 1), with very low LOD and LOQ values, 1.0 µg/kg, 5.0 µg/kg, respectively. Excellent repeatability and reproducibility were also achieved. This highly sensitive and robust LC-MS/MS technique was applied successfully for the detection and quantification of MSG in a wide variety of foodstuffs...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29329849/thermal-degradation-of-chloramphenicol-in-model-solutions-spiked-tissues-and-incurred-samples
#6
Lei Tian, Stéphane Bayen
This study investigated the thermal degradation of a veterinary drug, chloramphenicol, in model solutions (water), as well as in spiked and incurred mussel tissues to understand its fate in the food supply chain. Thermal degradation kinetics followed a first-order model in water (e.g. degradation rate: 0.0018-0.0025 min-1 at 100 °C). After 1 h at 100 °C, the percentage degradation in spiked tissues (28.1 ± 7.1%) was significantly different (p < .05) from the values in water (14.2 ± 1...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29329840/determination-of-colistin-in-animal-tissues-egg-milk-and-feed-by-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry
#7
Qin Fu, Xiaowei Li, Kangni Zheng, Yuebin Ke, Yingyu Wang, Lina Wang, Fugen Yu, Xi Xia
A confirmatory method for the determination of colistin in animal tissues, egg, milk, and feed was developed and validated. Colistin A and colistin B were extracted from samples with the mixture of 10% trichloroacetic acid-acetonitrile and isolated with mixed-mode weak cation exchange cartridge. Analytes were separated from matrix components using ultra-high performance liquid chromatography, and detected with electrospray ionization on a triple quadrupole mass spectrometer. Mean recoveries ranged from 78.0% to 115...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29329837/the-fate-and-enantioselective-behavior-of-zoxamide-during-wine-making-process
#8
Xinglu Pan, Fengshou Dong, Na Liu, Youpu Cheng, Jun Xu, Xingang Liu, Xiaohu Wu, Zenglong Chen, Yongquan Zheng
The fate of zoxamide and its enantiomers were evaluated in detail during wine-making process. The enantiomers of zoxamide were separated and determined by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) after each processing procedure including washing, peeling, fermentation and clarification. Significant enantioselectivity was observed in all three treatments with the half-lives of R-zoxamide and S-zoxamide estimated to be 45.6 and 52.9 h in Group A, 45.0 and 52...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29329424/brassinosteroids-regulate-pavement-cell-growth-by-mediating-bin2-induced-microtubule-stabilization
#9
Xiaolei Liu, Qin Yang, Yuan Wang, Linhai Wang, Ying Fu, Xuelu Wang
Brassinosteroids (BRs), a group of plant steroid hormones, play important roles in regulating plant development. The cytoskeleton also affects key developmental processes and a deficiency in BR biosynthesis or signaling leads to abnormal phenotypes similar to those of microtubule-defective mutants. However, how BRs regulate microtubule and cell morphology remains unknown. Here, using liquid chromatography-tandem mass spectrometry, we identified tubulin proteins that interact with Arabidopsis BRASSINOSTEROID INSENSITIVE2 (BIN2), a negative regulator of BR responses in plants...
January 10, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29329347/salivary-proteomics-of-healthy-dogs-an-in-depth-catalog
#10
Sheila M F Torres, Eva Furrow, Clarissa P Souza, Jennifer L Granick, Ebbing P de Jong, Timothy J Griffin, Xiong Wang
OBJECTIVE: To provide an in-depth catalog of the salivary proteome and endogenous peptidome of healthy dogs, evaluate proteins and peptides with antimicrobial properties, and compare the most common salivary proteins and peptides between different breed phylogeny groups. METHODS: 36 healthy dogs without evidence of periodontal disease representing four breed phylogeny groups, based upon single nucleotide polymorphism haplotypes (ancient, herding/sighthound, and two miscellaneous groups)...
2018: PloS One
https://www.readbyqxmd.com/read/29329271/transketolase-is-identified-as-a-target-of-herbicidal-substance-%C3%AE-terthienyl-by-proteomics
#11
Bin Zhao, Jingqian Huo, Ning Liu, Jinlin Zhang, Jingao Dong
α-terthienyl is a natural phytotoxin isolated originally from Flaveria bidentis (L.) Kuntze. The bioassay presented here shows the strong herbicidal activity of α-terthienyl on Digitaria sanguinalis, Arabidopsis thaliana and Chlamydomonas reinhardtii. The α-terthienyl-induced response of A. thaliana at the protein level was analyzed at different times. Changes in the protein expression profiles were analyzed by two-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry (LC-MS/MS) mass spectrometry...
January 12, 2018: Toxins
https://www.readbyqxmd.com/read/29329091/determination-of-imm-h004-and-its-active-glucuronide-metabolite-in-rat-plasma-and-ringer-s-solution-by-ultra-performance-liquid-chromatography-tandem-mass-spectrometry
#12
Jianwei Jiang, Ziqian Zhang, Xiaowen Zou, Rui Wang, Jie Bai, Shengyu Zhao, Xiaoqing Fan, Li Sheng, Yan Li
IMM-H004 is a novel neuroprotective agent and its glucuronide metabolite IMM-H004G has similar protective effects against cerebral ischemic injury in vivo and in vitro. A specific and sensitive ultra-performance liquid chromatography-tandem mass spectrometry method was established and validated for determination of IMM-H004 and IMM-H004G simultaneously in rat plasma and Ringer's solution. Plasma samples containing IMM-H004, IMM-H004G and internal standard propranolol were prepared by direct protein precipitation in a sample-to-solvent ratio of 1:2:6 (plasma: water: acetonitrile), whereas no protein precipitation was required for Ringer's solution samples...
December 29, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29328669/urinary-methyleugenol-deoxyadenosine-adduct-as-potential-biomarker-of-methyleugenol-exposure-in-rats
#13
Yukun Feng, Saide Wang, Hui Wang, Ying Peng, Jiang Zheng
Methyleugenol (ME), a natural ingredient of several herbs and spices used in the human diet, is hepatocarcinogenic in rodents. Following metabolic activation to reactive carbocation intermediate, ME can bind covalently to DNA, which is directly associated with its carcinogenicity. In this work, a non-invasive approach to determine ME exposure was established by monitoring urinary ME-dA adduct. The developed method entails liquid-liquid extraction enrichment of the urinary ME-dA, incorporation of deuterated ME-dA as internal standard, and analysis by liquid chromatography coupled tandem mass spectrometry...
January 12, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29328498/an-uplc-ms-ms-method-for-simultaneous-determination-of-five-flavonoids-from-stellerachamaejasme-l-in-rat-plasma-and-its-application-to-a-pharmacokinetic-study
#14
Yun-Qing Li, Cheng-Jian Li, Lei Lv, Qing-Qing Cao, Xian Qian, Si Wei Li, Hui Wang, Liang Zhao
Stellerachamaejasme L. has been used as a Traditional Chinese Medicine for the treatment of scabies, tinea, stubborn skin ulcers, chronic tracheitis, cancer and tuberculosis. A sensitive and selective ultra-high liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous determination of five flavonoids (stelleranol, chamaechromone, neochamaejasmin A, chamaejasmine and isochamaejasmin) of Stellerachamaejasme L. in rat plasma. Chromatographic separation was accomplished on an Agilent Poroshell 120 EC-C18 column (2...
January 12, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29328366/expression-of-dna-damage-response-proteins-in-gastric-cancer-comprehensive-protein-profiling-and-histological-analysis
#15
Hiroki Arai, Ryuichi Wada, Kousuke Ishino, Mitsuhiro Kudo, Eiji Uchida, Zenya Naito
Gastric cancer is the third major cause of cancer-related mortality in Japan. The aim of this study was to identify a factor implicated in the biology of gastric cancer by comprehensive protein profiling. Protein profiling was carried out by liquid chromatography-tandem mass spectrometry, using formalin-fixed paraffin-embedded specimens of 17 gastric cancer cases. Pathway analysis and orthogonal partial least square-discriminant analysis suggested the significant expression of ribonucleoproteins, heterogeneous nuclear ribonucleoproteins, interleukin binding factor 2 (ILF2), KU70 and KU80, which are involved in DNA damage response (DDR)...
January 5, 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29327354/a-validated-liquid-chromatography-high-resolution-tandem-mass-spectrometry-method-for-the-simultaneous-quantitation-of-tryptophan-kynurenine-kynurenic-acid-and-quinolinic-acid-in-human-plasma
#16
Kathrin Arnhard, Florian Pitterl, Barbara Sperner-Unterweger, Dietmar Fuchs, Therese Koal, Herbert Oberacher
Tryptophan (TRP) catabolism via the kynurenine pathway is considered to represent a major link between inflammation and various diseases, including neurodegenerative disorders, depression, schizophrenia, multiple sclerosis, cardiovascular disease, and cancer. The kynurenine pathway and levels of TRP and its metabolites kynurenine (KYN), kynurenic acid (KYNA) and quinolinic acid (QUIN) are well regulated under physiological conditions but may be altered as part of the activated immune response. A simple, sensitive and specific liquid chromatography-time of flight mass spectrometry method was developed for determining levels of the four compounds in human plasma samples...
January 12, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29326871/quantification-of-the-enzyme-activities-of-iduronate-2-sulfatase-n-acetylgalactosamine-6-sulfatase-and-n-acetylgalactosamine-4-sulfatase-using-liquid-chromatography-tandem-mass-spectrometry
#17
Ryuichi Mashima, Mari Ohira, Torayuki Okuyama, Akiya Tatsumi
Mucopolysaccharidosis (MPS) is a genetic disorder characterized by the accumulation of glycosaminoglycans in the body. Of the multiple MPS disease subtypes, several are caused by defects in sulfatases. Specifically, a defect in iduronate-2-sulfatase (ID2S) leads to MPS II, whereas N-acetylgalactosamine-6-sulfatase (GALN) and N-acetylgalactosamine-4-sulfatase (ARSB) defects relate to MPS IVA and MPS VI, respectively. A previous study reported a combined assay for these three disorders in a 96-well plate using a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based technique (Kumar et al...
March 2018: Molecular Genetics and Metabolism Reports
https://www.readbyqxmd.com/read/29326070/enhanced-protein-identification-using-graphite-modified-maldi-plates-for-offline-lc-maldi-ms-ms-bottom-up-proteomics
#18
Anthony Maus, Rudolph Mignon, Franco Basile
The use of offline liquid chromatography-matrix assisted laser desorption/ionization (LC-MALDI) tandem mass spectrometry (MS/MS) for bottom-up proteomics offers advantages in terms of cost, ease of use, and the time-decoupled nature of the separation step and the mass analysis. A method was developed to improve the capabilities of LC-MALDI-MS/MS in terms of protein identification in a bottom-up proteomic workflow. Enhanced protein identification is achieved by an increase in the MALDI signal intensity of the precursor peptides brought about by coating the MALDI plate with a thin film of graphite powder...
January 8, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29325058/serum-carnitine-metabolites-and-incident-type-2-diabetes-mellitus-in-patients-with-suspected-stable-angina-pectoris
#19
Elin Strand, Eirik W Rebnord, Malin R Flygel, Vegard Lysne, Gard F T Svingen, Grethe S Tell, Kjetil H Løland, Rolf K Berge, Asbjørn Svardal, Ottar Nygård, Eva R Pedersen
Context: Carnitine and its metabolites are centrally involved in fatty acid metabolism. While elevated circulating concentrations have been observed in obesity and insulin resistance, prospective studies examining whether these metabolites are associated with incident type 2 diabetes mellitus (T2D) are sparse. Objective: We performed a comprehensive evaluation of metabolites along the carnitine pathway in relation to incident T2D. Design: A total of 2519 patients (73...
January 9, 2018: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29324280/effective-phospholipids-removing-microelution-solid-phase-extraction-lc-ms-ms-method-for-simultaneous-plasma-quantification-of-aripiprazole-and-dehydro-aripiprazole-application-to-human-pharmacokinetic-studies
#20
Aneta Wojnicz, Carmen Belmonte, Dora Koller, Ana Ruiz-Nuño, Manuel Román, Dolores Ochoa, Francisco Abad Santos
A simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for simultaneous quantification of aripiprazole and its active metabolite, dehydro-aripiprazole, in human plasma. Stable isotopically labeled aripiprazole, aripiprazole-D8, has been used as the internal standard (IS) for both analytes. Only 200 μl of human plasma was needed for analyte extraction, using effective phospholipids-eliminating three-step microelution-solid-phase extraction (SPE, Oasis PRiME HLB 96-well μElution Plate)...
December 28, 2017: Journal of Pharmaceutical and Biomedical Analysis
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