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https://www.readbyqxmd.com/read/29145098/a-novel-simplified-strategy-of-relative-quantification-n-glycan-quantitative-glycomics-using-electrospray-ionization-mass-spectrometry-through-the-stable-isotopic-labeling-by-transglycosylation-reaction-of-mutant-enzyme-endo-m-n175q
#1
Qing Shi, Ryugo Hashimoto, Tadamune Otsubo, Kiyoshi Ikeda, Kenichiro Todoroki, Hajime Mizuno, Dongri Jin, Toshimasa Toyo'oka, Zhe Jiang, Jun Zhe Min
The lack of a highly sensitive and simple method for the quantitative analysis of glycan has impeded the exploration of protein glycosylation patterns (glycomics), evaluation of antibody drug stability, and screening of disease glycan biomarkers. In this study, we describe a novel and simplified quantitative glycomics strategy. Quantitation by mutant enzyme reaction stable isotope labeling (QMERSIL) to label the N-glycans with either a nondeuterated (d0-) or deuterated (d8-) 4-(2,4-Dinitro-5-piperazin-1-yl-phenyl)-1,1-dimethyl-piperazin-1-ium (MPDPZ)-Boc-asparaginyl-N-acetyl-d-glucosamine (Boc-Asn-GlcNAc) acceptor of a positive charge structure through the glycosynthase (Endo-M-N175Q) transglycosylation reaction with mass spectrometry facilitates comparative glycomics...
November 12, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29145097/lc-esi-ms-ms-determination-of-defactinib-a-novel-fak-inhibitor-in-mice-plasma-and-its-application-to-a-pharmacokinetic-study-in-mice
#2
Gurulingappa Hallur, Natarajan Tamizharasan, Suresh P Sulochana, Neeraj Kumar Saini, Mohd Zainuddin, Ramesh Mullangi
A sensitive, specific, selective and rapid LC-ESI-MS/MS method has been developed and validated for the quantification of defactinib in mice plasma using (13)C3,(15)N-tofacitinib as an internal standard (I.S.). Sample preparation was accomplished through a liquid-liquid extraction process. Baseline chromatographic resolution of defactinib and the I.S. was achieved on an Atlantis dC18 column using an isocratic mobile phase comprising 0.2% formic acid in water and acetonitrile (25:75, v/v) delivered at a flow rate of 0...
November 10, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29145026/lc-ms-ms-quantification-of-felinine-metabolites-in-tissues-fluids-and-excretions-from-the-domestic-cat-felis-catus
#3
Ayami Futsuta, Wataru Hojo, Tamako Miyazaki, Tetsuro Yamashita, Masao Miyazaki
Domestic cat urine contains large concentrations of the unusual amino acid, felinine (2-amino-7-hydroxy-5,5-dimethyl-4-thiaheptanoic acid). A felinine derivative, 3-mercapt-3-methylbutanol is a potential scent signal for letting other animals know that the scent owners are cats. 3-Methylbutanol-glutathione (MBG) is an upstream precursor of the biosynthetic pathway of felinine that may be produced in hepatocytes by conjugation of glutathione with isopentenyl pyrophosphate, an intermediate for cholesterol biosynthesis...
November 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29145024/direct-analysis-of-ethylene-glycol-in-human-serum-on-the-basis-of-analyte-adduct-formation-and-liquid-chromatography-tandem-mass-spectrometry
#4
Marek Dziadosz
The aim of this work was to develop a fast, cost-effective and time-saving liquid chromatography-tandem mass spectrometry (LC-MS/MS) analytical method for the analysis of ethylene glycol (EG) in human serum. For these purposes, the formation/fragmentation of an EG adduct ion with sodium and sodium acetate was applied in the positive electrospray mode for signal detection. Adduct identification was performed with appropriate infusion experiments based on analyte solutions prepared in different concentrations...
November 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29144740/identification-synthesis-and-safety-assessment-of-thidiazuron-1-phenyl-3-1-2-3-thidiazol-5-yl-urea-and-its-metabolites-in-kiwifruits
#5
Zhiwei Zhang, Haihua Yang, Zhenhong Gao, Yahong Yuan, Jing Dong, Yuan Wang, Tianli Yue
Quality of kiwifruit became worse due to the abuse of plant growth regulators (PGRs). The safety of the fruits treated with PGRs are also worried by consumers. Therefore, the present study analysed the structure of thidiazuron (TDZ, (1-phenyl-3-(1,2,3-thidiazol-5-yl)urea)) (1) and its metabolites of bio-transformation in kiwifruits by using liquid chromatography hybrid ion trap time-of-flight mass spectrometry (LC-IT-TOF-MS). Standard compounds were also synthesized and used for structural identification of those metabolites...
November 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29143972/determination-of-tubuloside-b-by-lc-ms-ms-and-its-application-to-a-pharmacokinetic-study-in-rats
#6
Shenbao Yang, Ruiying Qu, Peilu Sun, Shan Xiong, Siyi Yan, Zhipeng Deng
Tubuloside B, a novel neuroprotective phenylethanoid, is a major active constituent of Cistanche tubulosa and Cistanche deserticola. A specific and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantification of tubuloside B in rat plasma. Sample preparation was conducted through a protein-precipitation extraction with methanol using tubuloside A as internal standard (IS). Chromatographic separation was achieved by using a Capcell Pak C18 column (2...
November 16, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29143924/blood-plasma-biomarkers-of-citrinin-and-ochratoxin-a-exposure-in-young-adults-in-bangladesh
#7
Nurshad Ali, Khaled Hossain, Gisela H Degen
Citrinin (CIT) and Ochratoxin A (OTA) are nephrotoxic mycotoxins which can co-occur in food commodities, resulting in internal exposure. Studies in many countries reported on the presence of OTA in human blood; however, such biomonitoring data for CIT is still scarce. This study was conducted to characterize both CIT and OTA biomarker levels in plasma of volunteers since food analysis data are insufficient to assess human exposure in Bangladesh. In total 104 blood samples were collected from university students in 2013 (sampling 1: n = 64, midsummer) and 2014 (sampling 2: n = 40, end winter) for analysis of CIT and OTA and their metabolites HO-CIT and OTα by LC-MS/MS and HPLC-FD techniques, respectively...
November 16, 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/29143879/comparison-of-analytical-tools-appropriate-for-identification-of-proteinaceous-additives-in-historical-mortars
#8
Iva Krizova, Julia Schultz, Ivan Nemec, Radomir Cabala, Radovan Hynek, Stepanka Kuckova
Natural organic additives such as eggs, lard, resins, and oils have been added to mortars since ancient times, because the ancient builders knew of their positive effect on the mortar quality. The tradition of adding organic materials to mortars was commonly handed down only verbally for thousands years. However, this practice disappeared in the nineteenth century, when the usage of modern materials started. Today, one of the most recent topics in the industry of building materials is the reusing of natural organic materials and searching for the forgotten ancient recipes...
November 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29143878/sensitive-mass-spectrometric-assay-for-determination-of-15-deoxy-%C3%AE-12-14-prostaglandin-j2-and-its-application-in-human-plasma-samples-of-patients-with-diabetes
#9
Jakob Morgenstern, Thomas Fleming, Ivelina Kadiyska, Sebastian Brings, Jan Benedikt Groener, Peter Nawroth, Markus Hecker, Maik Brune
The determination of individual prostaglandins (PG) in humans is mainly performed in urine samples. The quantification of PGs in human plasma could improve the understanding of particular PG species under various physiological and pathological conditions. 15-Deoxy-Δ(12,14)-prostaglandin J2 (15d-PGJ2) is a dehydrated downstream product of PGD2 and is of high interest due to its recently discovered anti-inflammatory effects. Increasing availability of highly sensitive mass spectrometry allows the quantification of low abundant biomarkers like 15d-PGJ2 in human plasma samples...
November 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29143575/determination-of-illegal-adulteration-of-dietary-supplements-with-synthetic-hair-growth-compounds-by-uplc-and-lc-q-tof-ms
#10
Ji Hyun Lee, Gihaeng Kang, Han Na Park, Jihee Kim, Nam Sook Kim, Seongsoo Park, Sung-Kwan Park, Sun Young Baek, Hoil Kang
In this study, we developed a UPLC-PDA and LC-Q-TOF/MS method to identify and measure the following prohibited substances that may be found in dietary supplements: triaminodil, minoxidil, bimatoprost, alimemazine, diphenylcyclopropenone, α-tradiol, finasteride, methyltestosterone, spironolatone, flutamide, cyproterone, dutasteride, and testosterone 17-propionate.The method was validated according to International Conference on Harmonization guidelines in terms of specificity, linearity, accuracy, precision, LOD, LOQ, recovery, and stability...
November 16, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/29143334/pk-pd-modeling-of-flunixin-meglumine-in-a-kaolin-induced-inflammation-model-in-piglets
#11
O L Levionnois, T K Fosse, B Ranheim
Flunixin is marketed in several countries for analgesia in adult swine but little is known about its efficacy in piglets. Thirty-two piglets (6-8 days old) were randomized to receive placebo saline (n = 11, group CONTROL) or flunixin meglumine intravenously at 2.2 (n = 11, group MEDIUM) or 4.4 (n = 10, group HIGH) mg/kg, 10 hr after subcutaneous injection of kaolin in the left metacarpal area. A hand-held algometer was used to determine each piglet's mechanical nociceptive threshold (MNT) from both front feet up to 50 hr after treatment (cut-off value of 24...
November 16, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29142391/acacia-catechu-ethanolic-seed-extract-triggers-apoptosis-of-scc-25-cells
#12
Thangavelu Lakshmi, Devaraj Ezhilarasan, Upendra Nagaich, Rajagopal Vijayaragavan
Background: Acacia catechu Willd (Fabaceae), commonly known as catechu, cachou, and black cutch, has been studied for its hepatoprotective, antipyretic, antidiarrheal, hypoglycemic, anti-inflammatory, immunomodulatory, antinociceptive, antimicrobial, free radical scavenging, and antioxidant activities. Objective: We evaluated the cytotoxic activity of ethanol extract of A. catechu seed (ACS) against SCC-25 human oral squamous carcinoma cell line. Methods: Cytotoxic effect of ACS extract was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, using concentrations of 0...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29142051/improved-detection-of-her2-by-a-quasi-targeted-proteomics-approach-using-aptamer-peptide-probe-and-liquid-chromatography-tandem-mass-spectrometry
#13
Weixian Zhou, Feifei Xu, Danni Li, Yun Chen
BACKGROUND: Human epidermal growth factor receptor 2 (HER2)-positive breast cancer is a particularly aggressive type of the disease. To date, much evidence has indicated that accurate HER2 status detection is crucial for prognosis and treatment strategy selection. Thus, bioanalytical techniques for early and accurate detection of HER2 have the potential to improve patient care. Currently, the widely used immunohistochemical staining normally has problems with reproducibility and lack of standardization, resulting in poor concordance between laboratories...
November 15, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/29141708/sensitive-detection-of-neonicotinoid-insecticides-and-other-selected-pesticides-in-pollen-and-nectar-using-nanoflow-liquid-chromatography-orbitrap-tandem-mass-spectrometry
#14
David Moreno-González, Jaime Alcántara-Durán, Bienvenida Gilbert-López, Miriam Beneito-Cambra, Víctor M Cutillas, Łukasz Rajski, Antonio Molina-Díaz, Juan F García-Reyes
In this work, a new method based on nanoflow LC with high-resolution MS was developed for the determination ofeight pesticides in pollen and nectar samples, including neonicotinoid insecticides and other selected pesticides commonly found in bees and beeswax. Detection was undertaken with a hybrid quadrupole-Orbitrap mass spectrometer (Q Exactive™) equipped with a commercial nanospray ion source. The extraction of pesticides from pollen samples was performed by a modified micro-QuEChERS method scaled down to Eppendorf tubes, whereas nectar samples were simply diluted with a water–methanol (95 + 5, v/v) solution...
November 15, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29141195/determination-of-the-effect-of-pinellia-ternata-thunb-breit-on-nervous-system-development-by-proteomics
#15
Jian-Ya Xu, Chen Dai, Jin-Jun Shan, Tong Xie, Hui-Hui Xie, Ming-Ming Wang, Guang Yang
ETHNOPHARMACOLOGICAL RELEVANCE: Banxia (BX) is the dried tuber of Pinellia ternata (Thunb.) Breit., a commonly prescribed Chinese medicinal herb for the treatment of cough, phlegm, and vomiting in pregnant women. However, raw BX has been demonstrated to exert toxic effects on reproduction and the precise and comprehensive mechanisms remain elusive. AIM OF THE STUDY: We applied an iTRAQ (isobaric tags for relative and absolute quantitation, iTRAQ)-based proteomic method to explore the mechanisms of raw BX-induced fetal toxicity in mice...
November 12, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29140698/mutation-induced-deamidation-of-corneal-dystrophy-related-transforming-growth-factor-%C3%AE-induced-protein
#16
Nadia Sukusu Nielsen, Dennis Wilkens Juhl, Ebbe Toftgaard Poulsen, Marie V Lukassen, Emil Christian Poulsen, Michael W Risør, Carsten Scavenius, Jan Johannes Enghild
Mutations in the transforming growth factor β-induced protein (TGFBIp) cause phenotypically diverse corneal dystrophies, where protein aggregation in the cornea leads to severe visual impairment. Previous studies have shown a relationship between mutant-specific corneal dystrophy phenotypes and the thermodynamic stability of TGFBIp. Using LC-MS/MS and NMR, we investigated correlations between the structural integrity of disease-related mutants of the fourth FAS1 domain (FAS1-4) and deamidation of TGFBIp residue Asn622...
November 15, 2017: Biochemistry
https://www.readbyqxmd.com/read/29140273/larix-decidua-bark-as-a-source-of-phytoconstituents-an-lc-ms-study
#17
Valeria Baldan, Stefania Sut, Marta Faggian, Elena Dalla Gassa, Sara Ferrari, Gabriele De Nadai, Stefano Francescato, Gianni Baratto, Stefano Dall'Acqua
Larix decidua bark is a waste of the timber industry and is widely diffused in Northern Italy. This material can be considered a good source of antioxidants and phytoconstituents with possible use in cosmetic or nutraceutical products. In this study, simple extraction of larch bark was performed using mixtures of ethanol/water. Furthermore, the phytochemical composition of larch bark extract was studied using LC-MS(n) methods and the main constituents were identified as flavonoids, spiro-polyphenols, and procyanidins...
November 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29138943/contaminants-of-emerging-concern-in-surface-waters-in-barbados-west-indies
#18
Quincy A Edwards, Sergei M Kulikov, Leah D Garner-O'Neale, Chris D Metcalfe, Tamanna Sultana
Contaminants of emerging concern (CECs), including pharmaceuticals, artificial sweeteners, steroid hormones, and current-use pesticides have been detected in surface waters around the world, but to date, there have been no reports in the peer-reviewed literature on the levels of these classes of contaminants in freshwater resources in the Caribbean region. In the present study, multi-residue solid phase extraction (SPE) and liquid chromatography with tandem mass spectroscopy (LC-MS/MS) were used to analyze grab samples of surface waters collected from five different watersheds in Barbados, West Indies...
November 14, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29138881/a-harmonized-immunoassay-with-liquid-chromatography-mass-spectrometry-analysis-in-egg-allergen-determination
#19
Masaomi Nimata, Hideki Okada, Kei Kurihara, Tsukasa Sugimoto, Tsutomu Honjoh, Kazuhiko Kuroda, Takeo Yano, Hirofumi Tachibana, Masahiro Shoji
Food allergy is a serious health issue worldwide. Implementing allergen labeling regulations is extremely challenging for regulators, food manufacturers, and analytical kit manufacturers. Here we have developed an "amino acid sequence immunoassay" approach to ELISA. The new ELISA comprises of a monoclonal antibody generated via an analyte specific peptide antigen and sodium lauryl sulfate/sulfite solution. This combination enables the antibody to access the epitope site in unfolded analyte protein. The newly developed ELISA recovered 87...
November 14, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29138512/proteome-wide-identification-of-lysine-2-hydroxyisobutyrylation-reveals-conserved-and-novel-histone-modifications-in-physcomitrella-patens
#20
Zhiming Yu, Jun Ni, Wei Sheng, Zhikun Wang, Yuhuan Wu
Protein lysine 2-hydroxyisobutyrylation (Khib) is a newly identified post-translational modification found in animal and yeast cells. Previous research suggested that histone Khib is involved in male cell differentiation and plays a critical role in the regulation of chromatin functions in animals. However, information regarding protein Khib in plants is still limited. In this study, using a specific antibody and LC-MS/MS methods, we identified 11,976 Khib sites in 3,001 proteins in Physcomitrella patens. The bioinformatics analysis indicated that these Khib-modified proteins were involved in a wide range of molecular functions and cellular processes, and showed diverse subcellular localizations...
November 14, 2017: Scientific Reports
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