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https://www.readbyqxmd.com/read/28335578/exploring-the-antitumor-mechanism-of-high-dose-cytarabine-through-the-metabolic-perturbations-of-ribonucleotide-and-deoxyribonucleotide-in-human-promyelocytic-leukemia-hl-60-cells
#1
Zheng Li, Jian-Ru Guo, Qian-Qian Chen, Cai-Yun Wang, Wei-Jia Zhang, Mei-Cun Yao, Wei Zhang
Despite the apparent clinical benefits of high-dose cytarabine (Ara-C) over lower dose Ara-C in acute myeloid leukemia (AML) therapy, the mechanism behind high-dose Ara-C therapy remains uncertain. In this study, a LC-MS-based method was carried out to investigate the metabolic alteration of ribonucleotide and deoxyribonucleotide in human promyelocytic leukemia cells (HL-60) after treatment with Ara-C to reveal its antitumor mechanism. The metabolic results revealed that four nucleotides (ATP, ADP, CDP, and dCTP) could be used as potential biomarkers indicating the benefit of high-dose Ara-C over lower dose Ara-C treatment...
March 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28335036/validated-method-for-the-screening-and-quantification-of-baclofen-gabapentin-and-pregabalin-in-human-post-mortem-whole-blood-using-protein-precipitation-and-liquid-chromatography-tandem-mass-spectrometry
#2
Limon Nahar, Amy Smith, Rajan Patel, Rebecca Andrews, Sue Paterson
There has been a rapid increase in the number of prescriptions for baclofen (BLF), gabapentin (GBP) and pregabalin (PGL) in the UK since their introduction to therapy. Recent studies across the European Union and USA have shown the illicit abuse potential of these drugs and deaths have been observed. A simple, reliable and fully validated method was developed for the screening and quantification of BLF, GBP and PGL in human post-mortem (PM) blood. The analytes and their deuterated analogs as internal standard were extracted from blood using a single addition acetonitrile protein precipitation reaction followed by analysis using liquid chromatography-tandem mass spectrometry (LC-MS-MS) with triggered dynamic multiple reaction monitoring mode for simultaneous confirmation and quantification...
March 10, 2017: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/28335025/a-novel-lc-ms-ms-method-with-an-effective-antioxidant-for-the-determination-of-edaravone-a-free-radical-scavenger-in-dog-plasma-and-its-application-to-a-pharmacokinetic-study
#3
Feng Shao, Xiao-Ling Hu, Xin Liu, Mang-Ting Shan
The objective of this study was to investigate the stability of edaravone in dog plasma by using an added antioxidant stabilizer, with an ultimate goal of developing and validating a sensitive, reliable and robust LC-MS-MS method for determination of edaravone in plasma samples. Edaravone was unstable in plasma, but it presented a good stability performance in the plasma with sodium metabisulfite (SMB), an effective antioxidant. The blood sample was collected in the heparinized eppendorf tube containing SMB and plasma sample was deproteinized using acetonitrile containing 20 ng/mL of phenacetin (Internal standard)...
March 2, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28335023/qbd-driven-development-and-validation-of-liquid-chromatography-tandem-mass-spectrometric-method-for-the-quantitation-of-sildenafil-in-human-plasma
#4
M Saquib Hasnain, Shaukat Ali Ansari, Shireen Rao, Mohammad Tabish, Mukul Singh, Mohammad Syafiq Abdullah, Mohammed Tahir Ansari
The present work was employing the Quality by Design approach for the development and validation of a LC-MS-MS method to support the clinical advancement in determination of sildenafil in human plasma using lorazepam as an internal standard. Sample preparation involved solid phase extraction and calibration range observed between 3 and 1,700 ng/mL. The method was systematically optimized by employing Box-Behnken design and used mobile phase flow rate, pH and composition of mobile phase as the critical factors, and assessing the design for retention time and peak area as the responses...
February 27, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28334925/simultaneous-lc-ms-ms-determination-of-lopinavir-and-rifabutin-in-human-plasma
#5
Swati Jaiswal, Abhisheak Sharma, Mahendra Shukla, Jawahar Lal
Tuberculosis (TB) with human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome represents the most common infectious diseases worldwide. Anti-TB drugs are used concurrently with antiretroviral drug for treatment of TB-HIV co-morbidities. Due to lower risk of interaction with protease inhibitors, rifabutin is preferred over rifampicin in treatment of HIV and TB co-morbidity. A simple and specific liquid chromatography tandem mass spectrometry method was developed for quantification of rifabutin (RBT) and lopinavir (LPV) simultaneously in human plasma...
March 3, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28334841/oral-fluid-testing-for-cocaine-analytical-evaluation-of-two-point-of-collection-drug-screening-devices
#6
Juliana Nichterwitz Scherer, Taís Regina Fiorentin, Tanara Rosangela Vieira Sousa, Renata Pereira Limberger, Flavio Pechansky
The use of point-of-collection testing (POCT) devices for drugs of abuse in oral fluid (OF) is an advantageous tool that has been used for different purposes-particularly traffic enforcement. However, even with the widespread report of cocaine consumption, the reliability of POCT devices has been reported in different magnitudes. This study evaluated the reliability of two POCT devices for the detection of cocaine in OF samples of 110 cocaine users: (i) the DDS2™ (cutoff = 30 ng/mL) and (ii) the Multi-Drugs Multi-Line-Twist Screen Test Device™ (MDML) (cutoff = 20 ng/mL)...
February 27, 2017: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/28334817/stability-of-synthetic-cannabinoids-in-biological-specimens-analysis-through-liquid-chromatography-tandem-mass-spectrometry
#7
Chelsea Fort, Thomas Jourdan, Jesse Kemp, Byron Curtis
The focus of this study was to determine the stability of four synthetic cannabinoids, XLR-11, UR-144, AB-Pinaca and AB-Fubinaca in biological specimens for the purpose of casework processing prioritization. The study used human whole blood spiked with the compounds of interest to mimic real forensic laboratory samples submitted for synthetic cannabinoid analysis. The spiked whole blood specimens were incubated under one of three temperature conditions: room or ambient (22°C), refrigerated (4°C) and frozen (-20°C) for a period of 12 weeks...
February 18, 2017: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/28333993/integrated-transcriptomic-and-proteomic-analysis-of-the-molecular-cargo-of-extracellular-vesicles-derived-from-porcine-adipose-tissue-derived-mesenchymal-stem-cells
#8
Alfonso Eirin, Xiang-Yang Zhu, Amrutesh S Puranik, John R Woollard, Hui Tang, Surendra Dasari, Amir Lerman, Andre J van Wijnen, Lilach O Lerman
BACKGROUND: Mesenchymal stromal/stem cell (MSC) transplantation is a promising therapy for tissue regeneration. Extracellular vesicles (EVs) released by MSCs act as their paracrine effectors by delivering proteins and genetic material to recipient cells. To assess how their cargo mediates biological processes that drive their therapeutic effects, we integrated miRNA, mRNA, and protein expression data of EVs from porcine adipose tissue-derived MSCs. METHODS: Simultaneous expression profiles of miRNAs, mRNAs, and proteins were obtained by high-throughput sequencing and LC-MS/MS proteomic analysis in porcine MSCs and their daughter EVs (n = 3 each)...
2017: PloS One
https://www.readbyqxmd.com/read/28333449/epigenetic-modulation-of-endophytic-eupenicillium-sp-lg41-by-a-histone-deacetylase-inhibitor-for-production-of-decalin-containing-compounds
#9
Gang Li, Souvik Kusari, Christopher Golz, Hartmut Laatsch, Carsten Strohmann, Michael Spiteller
An endophytic fungus, Eupenicillium sp. LG41, isolated from the Chinese medicinal plant Xanthium sibiricum, was subjected to epigenetic modulation using an NAD(+)-dependent histone deacetylase (HDAC) inhibitor, nicotinamide. Epigenetic stimulation of the endophyte led to enhanced production of two new decalin-containing compounds, eupenicinicols C and D (3 and 4), along with two biosynthetically related known compounds, eujavanicol A (1) and eupenicinicol A (2). The structures and stereochemistry of the new compounds were elucidated by extensive spectroscopic analysis using LC-HRMS, NMR, optical rotation, and ECD calculations, as well as single-crystal X-ray diffraction...
March 23, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28333342/activity-of-faropenem-with-and-without-rifampicin-against-mycobacterium-tuberculosis-evaluation-in-a-whole-blood-bactericidal-activity-trial
#10
Meera Gurumurthy, Rupangi Verma, Claire M Naftalin, Kim Hor Hee, Qingshu Lu, Kin Hup Tan, Simi Issac, Wenwei Lin, Angelia Tan, Kok-Yong Seng, Lawrence Soon-U Lee, Nicholas I Paton
Background: Faropenem has in vitro activity against Mycobacterium tuberculosis (Mtb) and shows synergy with rifampicin. We tested this in a whole-blood bactericidal activity (WBA) trial. Methods: We randomized healthy volunteers to receive a single oral dose of faropenem (600 mg) with amoxicillin/clavulanic acid (500/125 mg) ( n  =   8), rifampicin (10 mg/kg) ( n  =   14) or the combination rifampicin + faropenem + amoxicillin/clavulanic acid ( n  =   14)...
March 18, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28333250/the-complexity-of-minocycline-serum-protein-binding
#11
Jian Zhou, Brian T Tran, Vincent H Tam
Objectives: Serum protein binding is critical for understanding the pharmacology of antimicrobial agents. Tigecycline and eravacycline were previously reported to have atypical non-linear protein binding; the percentage of free fraction decreased with increasing total concentration. In this study, we extended the investigation to other tetracyclines and examined the factors that might impact protein binding. Methods: Different minocycline concentrations (0.5-50 mg/L) and perfusion media (saline, 0...
February 25, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28333238/clustering-of-pcos-like-traits-in-naturally-hyperandrogenic-female-rhesus-monkeys
#12
D H Abbott, B H Rayome, D A Dumesic, K C Lewis, A K Edwards, K Wallen, M E Wilson, S E Appt, J E Levine
STUDY QUESTION: Do naturally occurring, hyperandrogenic (≥1 SD of population mean testosterone, T) female rhesus monkeys exhibit traits typical of women with polycystic ovary syndrome (PCOS)? SUMMARY ANSWER: Hyperandrogenic female monkeys exhibited significantly increased serum levels of androstenedione (A4), 17-hydroxyprogesterone (17-OHP), estradiol (E2), LH, antimullerian hormone (AMH), cortisol, 11-deoxycortisol and corticosterone, as well as increased uterine endometrial thickness and evidence of reduced fertility, all traits associated with PCOS...
March 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28332626/decreased-16-0-20-4-phosphatidylinositol-level-in-the-post-mortem-prefrontal-cortex-of-elderly-patients-with-schizophrenia
#13
Junya Matsumoto, Hiroki Nakanishi, Yasuto Kunii, Yuki Sugiura, Dai Yuki, Akira Wada, Mizuki Hino, Shin-Ichi Niwa, Takeshi Kondo, Michihiko Waki, Takahiro Hayasaka, Noritaka Masaki, Hiroyasu Akatsu, Yoshio Hashizume, Sakon Yamamoto, Shinji Sato, Takehiko Sasaki, Mitsutoshi Setou, Hirooki Yabe
The etiology of schizophrenia includes phospholipid abnormalities. Phospholipids are bioactive substances essential for brain function. To analyze differences in the quantity and types of phospholipids present in the brain tissue of patients with schizophrenia, we performed a global analysis of phospholipids in multiple brain samples using liquid chromatography electrospray ionization mass/mass spectrometry (LC-ESI/MS/MS) and imaging mass spectrometry (IMS). We found significantly decreased 16:0/20:4-phosphatidylinositol (PI) levels in the prefrontal cortex (PFC) in the brains from patients with schizophrenia in the LC-ESI/MS/MS, and that the 16:0/20:4-PI in grey matter was most prominently diminished according to the IMS experiments...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28332413/interlaboratory-validation-of-an-lc-ms-ms-method-for-the-determination-of-melamine-and-cyanuric-acid-in-animal-feed
#14
Hildburg Fry, Kerstin Mietle, Eileen Mähnert, Sebastian Zinke, Mandy Schwieters, Emanuele Pydde, Angelika Preiß-Weigert
Melamine and cyanuric acid have been mixed illegally into food and feed to increase the nitrogen content which results in deceptively high protein contents. As a consequence a maximum level for melamine of 2.5 mg kg(-1) feed was established by the European Union under directive 2002/32/EC. A method is required to quantify melamine and cyanuric acid for control of the maximum level. Therefore Technical Committee (TC) 327 of the European Committee for Standardisation (CEN) commissioned the standardisation of a method for the analysis of melamine and cyanuric acid in animal feed...
March 23, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28332299/the-use-of-on-line-sfe-sfc-ms-ms-to-analyze-disease-biomarkers-in-dried-serum-spots-compared-with-serum-analysis-using-lc-ms-ms
#15
Makoto Suzuki, Shin Nishiumi, Takashi Kobayashi, Arata Sakai, Yosuke Iwata, Takato Uchikata, Yoshihiro Izumi, Takeshi Azuma, Takeshi Bamba, Masaru Yoshida
RATIONALE: The analytical stability and throughput of biomarker assays based on dried serum spots (DSS) are strongly dependent on the extraction process and determination method. In the present study, an on-line system based on supercritical fluid extraction-supercritical fluid chromatography coupled with tandem mass spectrometry (SFE-SFC/MS/MS) was established for analyzing the levels of disease biomarkers in DSS. METHODS: The chromatographic conditions were investigated using the ODS-EP, diol, and SIL-100A columns...
March 23, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28332095/identification-of-t-cell-target-antigens-in-glioblastoma-stem-like-cells-using-an-integrated-proteomics-based-approach-in-patient-specimens
#16
Carmen Rapp, Rolf Warta, Slava Stamova, Ali Nowrouzi, Christoph Geisenberger, Zoltan Gal, Saskia Roesch, Steffen Dettling, Simone Juenger, Mariana Bucur, Christine Jungk, Philip DaoTrong, Rezvan Ahmadi, Felix Sahm, David Reuss, Valentina Fermi, Esther Herpel, Volker Eckstein, Niels Grabe, Christoph Schramm, Markus A Weigand, Juergen Debus, Andreas von Deimling, Andreas Unterberg, Amir Abdollahi, Philipp Beckhove, Christel Herold-Mende
Glioblastoma (GBM) is a highly aggressive brain tumor and still remains incurable. Among others, an immature subpopulation of self-renewing and therapy-resistant tumor cells-often referred to as glioblastoma stem-like cells (GSCs)-has been shown to contribute to disease recurrence. To target these cells personalized immunotherapy has gained a lot of interest, e.g. by reactivating pre-existing anti-tumor immune responses against GSC antigens. To identify T cell targets commonly presented by GSCs and their differentiated counterpart, we used a proteomics-based separation of GSC proteins in combination with a T cell activation assay...
March 22, 2017: Acta Neuropathologica
https://www.readbyqxmd.com/read/28331956/enzyme-assisted-supercritical-fluid-extraction-an-alternative-and-green-technology-for-non-extractable-polyphenols
#17
Muhammad Mushtaq, Bushra Sultana, Sumia Akram, Farooq Anwar, Ahmad Adnan, Syed S H Rizvi
This contribution proposes an enzyme-assisted eco-friendly process for the extraction of non-extractable polyphenols (NEPPs) from black tea leftover (BTLO), an underutilized tea waste. BTLO hydrolyzed with various enzyme formulations was extracted using supercritical carbon dioxide and ethanol as co-solvent (SC-CO2 + EtOH). A conventional solvent extraction (CSE) was performed using EtOH + H2O (80:20, v/v) for comparison purposes. The results revealed that hydrolysis of BTLO with 2.9% (w/w) kemzyme at 45 °C and pH 5...
March 22, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28330783/influence-of-mdr1-cyp3a5-genetic-polymorphisms-on-trough-levels-and-therapeutic-response-of-imatinib-in-newly-diagnosed-patients-with-chronic-myeloid-leukemia
#18
Natarajan Harivenkatesh, Lalit Kumar, Sameer Bakhshi, Atul Sharma, Madhulika Kabra, Tirumurthy Velpandian, Ajay Gogia, Shivaram S Shastri, Nihar Ranjan Biswas, Yogendra Kumar Gupta
Polymorphisms in genes coding for imatinib transporters and metabolizing enzymes may affect imatinib pharmacokinetics and clinical response. Aim of this study was to assess the influence of polymorphisms in MDR1 and CYP3A5 genes on imatinib trough levels, cytogenetic and molecular response in patients with CML. Newly diagnosed patients with chronic-phase CML started on imatinib therapy were enrolled and followed up prospectively for 24 months. The following single nucleotide polymorphisms were genotyped; C1236T, C3435T, G2677T/A in MDR1 gene and A6986G in CYP3A5 gene...
March 18, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28330376/determination-of-air-pollution-related-biomarkers-of-exposure-in-urine-of-travellers-between-germany-and-china-using-liquid-chromatographic-and-liquid-chromatographic-mass-spectrometric-methods-a-pilot-study
#19
Xiao Wu, Jutta Lintelmann, Sophie Klingbeil, Jie Li, Hao Wang, Evelyn Kuhn, Sebastian Ritter, Ralf Zimmermann
OBJECTIVE: The influence of different exposures to PM2.5 (particulate matter with an aerodynamic diameter below 2.5 µm) on the concentrations of biomarkers of exposure and oxidative stress should be investigated. For this purpose urine samples from 9 individuals travelling from Germany to China were collected and analysed. MATERIALS: Robust LC and LC-MS/MS methods were established for the determination of biomarkers including 8-hydroxy-2'deoxyguanosine, malondialdehyde, F2α-isoprostanes, and hydroxylated polycyclic aromatic hydrocarbons...
March 23, 2017: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/28330259/identification-of-endophytic-bacillus-mojavensis-with-highly-specialized-broad-spectrum-antibacterial-activity
#20
B Jasim, S Sreelakshmi, Jyothis Mathew, E K Radhakrishnan
Biosynthetic adaptation of endophytic bacteria to chemically support host plant is very remarkable. Hence these organisms from medicinal plants are considered as highly valuable sources for natural products with diverse bioactivity. Their metabolite diversity and biosynthetic versatility have been increasingly explored for drug discovery. In this study, an endophytic Bacillus mojavensis with broad spectrum antibacterial properties has been analyzed for the chemical basis of its activity. By LC-MS/MS the organism was identified to have the biosynthetic ability to produce lipopeptides surfactin and fengycin...
December 2016: 3 Biotech
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