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https://www.readbyqxmd.com/read/29232754/-measurement-of-the-strength-of-human-musculus-orbicularis-oculi-using-video-analysis
#1
Anita Koschmieder, Phillip Pisowocki, Carmen Zietz, Rainer Bader, Oliver Stachs, Anselm Jünemann
Introduction Current methods to measure the of strength of the musculus orbicularis oculi are limited by high proneness to examiner error or by their complex and impracticable set-ups. Our aim was to develop a simple and practicable method to measure eye lid power via video analysis. Methods 50 healthy subjects were included. A deformable single use lid speculum was used. Forced maximum lid closure was recorded via a video slit lamp. Analysis of the video data was performed with open source tracking software...
December 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/29232752/simultaneous-determination-of-rosuvastatin-rosuvastatin-5%C3%A2-s-lactone-and-n-desmethyl-rosuvastatin-in-human-plasma-by-uplc-ms-ms-and-its-application-to-clinical-study
#2
Xue Bai, Xi-Pei Wang, Guo-Dong He, Bin Zhang, Min Huang, Jia-Li Li, Shi-Long Zhong
Objective A rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) assay was developed and validated for the simultaneous quantification of rosuvastatin (RST), rosuvastatin-5 S-lactone (RSTL), and N-desmethyl rosuvastatin (DM-RST) in human plasma. Methods Sample was prepared by liquid-liquid extraction with ethyl acetate from 100 μL acidulated buffered plasma. Then analytes were chromatographically separated using an Acquity UPLC HSS T3 column (3.0 mm×100 mm, 1...
December 12, 2017: Drug Research
https://www.readbyqxmd.com/read/29232750/-a-novel-technique-for-the-preparation-of-a-3-or-5-stranded-hamstring-graft-for-individualised-single-bundle-acl-or-pcl-reconstruction
#3
Philip Peter Rößler, Karl-Friedrich Schüttler, Turgay Efe
Objective Development of a preparation technique for hamstring tendons to ensure a maximum of intraoperative flexibility during individualised cruciate ligament surgery. Indications Primary ACL- and PCL-reconstruction as well as cases of later revision. Method Three- or five-stranded grafts can be obtained with the use of semitendinosus and gracilis tendons with fiber tape enforcement and use of a conventional femoral cortical suspensory fixation device. Fibre tape and knotting style ensure free motion of the continous loop...
December 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/29232714/cytomegalovirus-cmv-immune-monitoring-with-elispot-and-quantiferon-cmv-assay-in-seropositive-kidney-transplant-recipients
#4
Hyeyoung Lee, Ki Hyun Park, Ji Hyeong Ryu, Ae-Ran Choi, Ji Hyun Yu, Jihyang Lim, Kyungja Han, Sang Il Kim, Chul Woo Yang, Byung Ha Chung, Eun-Jee Oh
Although cytomegalovirus (CMV) specific cell-mediated immunity (CMI) has been suggested as a predictive marker for CMV infection, proper CMI monitoring strategy in CMV-seropositive recipients and optimal method are not defined. The aim of this study was to evaluate two interferon gamma release assays during early post-transplant period as a predictor of the development of CMV infection in CMV-seropositive patients. A total of 124 CMV-seropositive recipients who received kidney transplantation from CMV-seropositive donor were prospectively examined...
2017: PloS One
https://www.readbyqxmd.com/read/29232675/evaluation-of-novel-anticaries-adhesive-in-a-secondary-caries-animal-model
#5
Tianmu Wu, Bolei Li, Xuedong Zhou, Yao Hu, Huajin Zhang, Yingkang Huang, Hockin H K Xu, Qiang Guo, Mingyun Li, Mingye Feng, Xian Peng, Michael D Weir, Lei Cheng, Biao Ren
We investigated the anticaries properties of an adhesive containing dimethylaminododecyl methacrylate (DMADDM) in vivo via a secondary caries animal model. Cavities were prepared in the maxillary first molars of Wistar rats. DMADDM-containing adhesives were applied on one side and commercial adhesives on the opposite side as a control. After a 3-week feeding period to induce secondary caries, the molars were harvested for the evaluation of the secondary caries. Lesion depth (LD) and mineral loss (ML) were measured via a micro-CT method, and a modified Keyes scoring method yielded scores for the caries lesions...
December 13, 2017: Caries Research
https://www.readbyqxmd.com/read/29232650/internal-validation-of-two-new-retrotransposons-based-kits-innoquant%C3%A2-hy-and-innotyper%C3%A2-21-at-a-forensic-lab
#6
Cátia Martins, Paulo Miguel Ferreira, Raquel Carvalho, Sandra Cristina Costa, Carlos Farinha, Luísa Azevedo, António Amorim, Manuela Oliveira
Obtaining a genetic profile from pieces of evidence collected at a crime scene is the primary objective of forensic laboratories. New procedures, methods, kits, software or equipment must be carefully evaluated and validated before its implementation. The constant development of new methodologies for DNA testing leads to a steady process of validation, which consists of demonstrating that the technology is robust, reproducible, and reliable throughout a defined range of conditions. The present work aims to internally validate two new retrotransposon-based kits (InnoQuant® HY and InnoTyper® 21), under the working conditions of the Laboratório de Polícia Científica da Polícia Judiciária (LPC-PJ)...
November 29, 2017: Forensic Science International
https://www.readbyqxmd.com/read/29232646/determination-of-uv-filters-in-human-breast-milk-using-turbulent-flow-chromatography-and-babies-daily-intake-estimation
#7
Daniel Molins-Delgado, María Del Mar Olmo-Campos, Gemma Valeta-Juan, Vanessa Pleguezuelos-Hernández, Damià Barceló, M Silvia Díaz-Cruz
UV filters (UV-Fs) are a group of hormonally active chemical compounds used to protect against the deleterious effects of UVA and UVB solar radiation, which are currently present in most consumer goods (personal care products, plastics, fabrics, paints, etc). Last years the concern about these emerging contaminants has been on the rise, and increasing efforts are being taken in order to properly asses the hazard that the exposure to these compounds in the early stages of life may pose. In this study, a new method for the analysis of 11 UV-Fs residues in human breast milk samples has been developed...
December 9, 2017: Environmental Research
https://www.readbyqxmd.com/read/29232633/carbazole-alkaloids-from-clausena-hainanensis-with-their-potential-antiproliferative-activities
#8
Yan-Lei Ma, Yan-Ping Liu, Chao Zhang, Wan-Hui Zhao, Shi Shi, Dan-Nv He, Pan Zhang, Xiao-Hong Liu, Ting-Ting Han, Yan-Hui Fu
Five new carbazole alkaloids, clausehainanines A-E (1-5), together with seven known analogues (6-12) were isolated from the stems and leaves of C. hainanensis. Their structures were elucidated by extensive spectroscopic methods. Among them, compounds 1-5 were an unusual type of carbazole alkaloids, possessing diverse isopentenyl derivatives as substituents at C-2. All isolated compounds were evaluated for their antiproliferative activities against five human cancer cell lines: HL-60, SMMC-7721, A-549, MCF-7 and SW480 in vitro...
December 5, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29232614/evaluation-of-carbon-nanotube-based-copper-nanoparticle-composite-for-the-efficient-detection-of-agroviruses
#9
Muhammad Ali Tahir, Sadia Z Bajwa, Shahid Mansoor, Rob W Briddon, Waheed S Khan, Brian E Scheffler, Imran Amin
We report a biosensor that combines the construction of a three-dimensional nanocomposite with electrochemical methods for the detection of viruses in plants. This is the first report, where carbon nanotubes are used as a conductive frame to anchor highly electrolytic agglomerates of copper nanoparticles to detect agroviruses. Morphological analysis of nanocomposite revealed the presence of carbon nanotubes having a diameter of 50-100nm with copper nanoparticles of 20-100nm, attached in the form of bunches...
December 5, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29232607/simultaneous-uplc-ms-ms-analysis-of-two-stable-isotope-labeled-versions-of-sucrose-in-mouse-plasma-and-brain-samples-as-markers-of-blood-brain-barrier-permeability-and-brain-vascular-space
#10
Ekram Ahmed Chowdhury, Faleh Alqahtani, Raktima Bhattacharya, Reza Mehvar, Ulrich Bickel
Blood Brain Barrier (BBB) permeability is frequently compromised in the course of diseases affecting the central nervous system (CNS). Sucrose is a low molecular weight, hydrophilic marker with slow permeability at the naive BBB and therefore one of the widely used indicators of barrier integrity. Our laboratory recently developed a highly sensitive UPLC-MS/MS method for stable isotope labeled [13C12]sucrose in biological matrices. Correction of total brain concentration for contribution of intravascular space is required in such experiments in order to accurately measure BBB permeability, and it is often accomplished by vascular perfusion with buffer solutions prior to brain sampling...
December 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29232606/microwave-assisted-synthesis-for-a2e-and-development-of-lc-esi-ms-method-for-quantification-of-ocular-bisretinoids-in-human-retina
#11
A Kotnala, S Senthilkumari, N Halder, A Kumar, T Velpandian
PURPOSE: To develop a microwave assisted method for the rapid synthesis of A2E and also to develop a method to quantify N-retinylidene-N-retinylethanolamine(A2E), all-trans retinal dimer (ATRD), A2-glycerophospho ethanolamine (A2GPE), dihydropyridine phosphatidyl ethanolamine (A2DHPE) and monofuran A2E (MFA2E) in age matched retina. METHODS: The development of microwave assisted synthesis of A2E, its purification and characterization for its utility in quantification in human retina...
November 26, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29232605/correlating-charge-heterogeneity-data-generated-by-agarose-gel-isoelectric-focusing-and-ion-exchange-chromatography-methods
#12
Babita Saxena Parekh, Arvind Srivastava, Shanmuuga Sundaram, Ming Ching-Heish, Joel Goldstein, Michael Barry, Qinwei Zhou
An isoelectric focusing method (IEF) has been used to assess the charge heterogeneity profile of a monoclonal antibody during the early stages of product development. A more precise and sensitive ion exchange chromatography (IEC/CEX) method was developed and implemented as development progressed and was used concurrently with IEF for lot release and to monitor charge heterogeneity. Charge variants resolved by both methods (IEC and IEF) were purified and characterized. Tryptic peptide mapping and N- linked oligosaccharide profile analyses of the IEC and IEF fractions indicated a structural correlation between the charge variants separated by these two methods...
December 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29232590/mode-repulsion-of-ultrasonic-guided-waves-in-rails
#13
Philip W Loveday, Craig S Long, Dineo A Ramatlo
Accurate computation of dispersion characteristics of guided waves in rails is important during the development of inspection and monitoring systems. Wavenumber versus frequency curves computed by the semi-analytical finite element method exhibit mode repulsion and mode crossing which can be difficult to distinguish. Eigenvalue derivatives, with respect to the wavenumber, are used to investigate these regions. A term causing repulsion between two modes is identified and a condition for two modes to cross is established...
November 24, 2017: Ultrasonics
https://www.readbyqxmd.com/read/29232570/development-of-an-orthosis-for-simultaneous-three-dimensional-correction-of-clubfoot-deformity
#14
Kanwaljit S Khas, Pulak M Pandey, Alok R Ray
BACKGROUND: Clubfoot is a three-dimensional deformity of the foot in which the foot is twisted in three mutually perpendicular planes from the normal shape of the foot. Of the various treatment methods that are available to manage clubfoot, non-operative approaches are preferred. The conventional non-operative method of treatment is to apply a series of casts to the infant's clubfoot to gradually manipulate its position. However, prolonged use of casts can result in skin rash, skin dehydration and ulcers on the soft skin of an infant...
December 6, 2017: Clinical Biomechanics
https://www.readbyqxmd.com/read/29232525/a-modified-ficoll-paque-gradient-method-for-isolating-mononuclear-cells-from-the-peripheral-and-umbilical-cord-blood-of-humans-for-biobanks-and-clinical-laboratories
#15
Yanjuan Jia, Hui Xu, Yonghong Li, Chaojun Wei, Rui Guo, Fang Wang, Yu Wu, Jing Liu, Jing Jia, Junwen Yan, Xiaoming Qi, Yuanting Li, Xiaoling Gao
Although the Ficoll-Paque method is classically used to isolate peripheral blood mononuclear cells (PBMCs), modifications in this method are required for a more rapid and economic output for biobanks and clinical laboratories, particularly in developing countries. In this study, we addressed this issue by modifying the Ficoll-Paque method for the isolation of PBMCs or mononuclear cells from the peripheral and the umbilical cord blood of healthy and diseased (infected, anemic, and chronic obstructive pulmonary disease) adult individuals...
December 12, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/29232519/using-density-based-indexes-and-wavefunction-methods-for-the-description-of-excited-states-excited-state-proton-transfer-reactions-as-test-case
#16
Juan Sanz García, Federica Maschietto, Marco Campetella, Ilaria Ciofini
To provide tools to interpret photochemical reactions, in this paper we demonstrate how a recently developed density-based index (DCT), up to now used in conjunction with Time Dependent Density Functional Theory methods, can be extended to multi-configurational methods. This index can guide chemists in the interpretation of photochemical reactions providing a measure of the spatial extent of a photo-induced charge transfer and, more generally, of charge transfer phenomena. This qualitative and quantitative description can be particularly relevant in the case of multi-configurational calculations providing a simple tool for the interpretation of their complex outputs...
December 12, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29232516/highly-enantioselective-synthesis-of-propargyl-amides-through-rh-catalyzed-asymmetric-hydroalkynylation-of-enamides-scope-mechanism-and-origin-of-selectivity
#17
Xiao-Yan Bai, Wen-Wen Zhang, Qian Li, Bi-Jie Li
Chiral propargyl amides are particularly useful structural units in organic synthesis. The enantioselective synthesis of propargyl amide is highly desirable. Conventional approach involves the use of stoichiometric amount of metal reagent or chiral auxiliary. In comparison, direct alkynylation with terminal alkyne is attractive because it avoids the use of stoichiometric organometallic reagent. The asymmetric coupling of aldehyde, amine and alkyne (A3-coupling) provide an efficient method for the synthesis of N-alkyl and N-aryl substituted propargyl amines, but this strategy is not amenable for the direct enantioselective synthesis of propargyl amide...
December 12, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29232507/interfacial-deposition-of-ru-ii-bipyridine-dicarboxylate-complexes-by-ligand-substitution-for-applications-in-water-oxidation-catalysis
#18
Degao Wang, Seth L Marquard, Ludovic Troian-Gautier, Matthew V Sheridan, Benjamin D Sherman, Ying Wang, Michael S Eberhart, Byron H Farnum, Christopher J Dares, Thomas J Meyer
Water oxidation is a critical step in artificial photosynthesis and provides the protons and electrons used in reduction reactions to make solar fuels. Significant advances have been made in the area of molecular water oxidation catalysts with a notable breakthrough in the development of Ru(II) complexes that use a planar "bda" ligand (bda is 2,2'-bipyridine-6,6'-dicarboxylate). These Ru(II)(bda) complexes show lower overpotentials for driving water oxidation making them ideal for light-driven applications with a suitable chromophore...
December 12, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29232492/use-of-split-free-nlc-ms-hrms-interface-to-improve-the-detection-of-%C3%AE-cobratoxin-in-equine-plasma-for-doping-control
#19
Ludovic Bailly-Chouriberry, Patrice Garcia, Florence Cormant, Benoit Loup, Marie-Agnès Popot, Yves Bonnaire
Cobra (Naja naja kaouthia) venom contains a toxin called α-cobratoxin (α-Cbtx) containing 71 amino acids (MW 7821 Da) with a reported analgesic power greater than morphine. In 2013, the first analytical method for the detection of α-Cbtx in equine plasma was developed by Bailly-Chouriberry et al. allowing the confirmation of the presence of α-Cbtx at low concentrations (1-5 ng/mL or 130-640 fmol/mL) in plasma samples. In order to increase the method sensitivity and therefore to improve the detection of α-Cbtx in post-administration plasma samples, a nano-liquid chromatography - mass spectrometry/high resolution mass spectrometry (nLC-MS/HRMS) method was developed...
December 12, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29232489/achieving-biocompatible-sabre-an-in-vitro-cytotoxicity-study
#20
Simon Duckett, Anand Manoharan, Peter J Rayner, Michael J Burns, Wissam Iali, V Hugh Perry
Production of a biocompatible hyperpolarized bolus by Signal Amplification By Reversible Exchange (SABRE) could open the door to simple clinical diagnosis via magnetic resonance imaging. Essential to successful progression to pre-clinical/clinical applications is the determination of the toxicology profile of the SABRE reaction mixture. Here we exemplify the cytotoxicity of the SABRE approach using in vitro cell assay. We conclude that the main cause of observed toxicity is due to the SABRE catalyst. Thus we develop two catalyst removal methods, one involving deactivation and ion-exchange chromatography and the second biphasic catalysis...
December 12, 2017: ChemMedChem
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