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https://www.readbyqxmd.com/read/29458214/effect-of-tetrahedral-dna-nanostructures-on-proliferation-and-osteo-odontogenic-differentiation-of-dental-pulp-stem-cells-via-activation-of-the-notch-signaling-pathway
#1
Mi Zhou, Nan-Xin Liu, Si-Rong Shi, Yong Li, Qi Zhang, Quan-Quan Ma, Tao-Ran Tian, Wen-Juan Ma, Xiao-Xiao Cai, Yun-Feng Lin
Dental pulp stem cells (DPSCs) derived from the human dental pulp tissue have multiple differentiation capabilities, such as osteo/odontogenic differentiation. Therefore, DPSCs are deemed as ideal stem cell sources for tissue regeneration. As new nanomaterials based on DNA, tetrahedral DNA nanostructures (TDNs) have tremendous potential for biomedical applications. Here, the authors aimed to explore the part played by TDNs in proliferation and osteo/odontogenic differentiation of DPSCs, and attempted to investigate if these cellular responses could be driven by activating the canonical Notch signaling pathway...
February 16, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29458187/mri-techniques-to-measure-arterial-and-venous-cerebral-blood-volume
#2
Jun Hua, Peiying Liu, Tae Kim, Manus Donahue, Swati Rane, J Jean Chen, Qin Qin, Seong-Gi Kim
The measurement of cerebral blood volume (CBV) has been the topic of numerous neuroimaging studies. To date, however, most in vivo imaging approaches can only measure CBV summed over all types of blood vessels, including arterial, capillary and venous vessels in the microvasculature (i.e. total CBV or CBVtot). As different types of blood vessels have intrinsically different anatomy, function and physiology, the ability to quantify CBV in different segments of the microvascular tree may furnish information that is not obtainable from CBVtot, and may provide a more sensitive and specific measure for the underlying physiology...
February 16, 2018: NeuroImage
https://www.readbyqxmd.com/read/29458080/transcriptomic-and-phenotypic-profiling-in-developing-zebrafish-exposed-to-thyroid-hormone-receptor-agonists
#3
Derik E Haggard, Pamela D Noyes, Katrina M Waters, Robert L Tanguay
There continues to be a need to develop in vivo high-throughput screening (HTS) and computational methods to screen chemicals for interaction with the estrogen, androgen, and thyroid pathways and as complements to in vitro HTS assays. This study explored the utility of an embryonic zebrafish HTS approach to identify and classify endocrine bioactivity using phenotypically-anchored transcriptome profiling. Transcriptome analysis was conducted on zebrafish embryos exposed to 25 estrogen-, androgen-, or thyroid-active chemicals at concentrations that elicited adverse malformations or mortality at 120 hours post-fertilization in 80% of animals exposed...
February 16, 2018: Reproductive Toxicology
https://www.readbyqxmd.com/read/29458050/investigation-of-metabolic-stability-of-the-novel-alk-inhibitor-brigatinib-by-liquid-chromatography-tandem-mass-spectrometry
#4
Hany W Darwish, Adnan A Kadi, Mohamed W Attwa, Halah S Almutairi
Brigatinib (BGB) belongs to a class of drugs called ALK inhibitor. On April 28, 2017, BGB has been approved by U.S. FDA for use in metastatic ALK-positive NSCLC. A fast, specific, sensitive and validated LC-MS/MS method was developed for the quantification of BGB in human plasma matrix. This method was applied successfully to study metabolic stability of BGB. Reversed phase (C18 column) and isocratic binary mobile phase (55% 0.1% formic acid: 45% ACN) were used for chromatographic separation of BGB and ponatinib (IS)...
February 16, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29458034/fluorescence-linked-immunosorbent-assay-for-detection-of-phenanthrene-and-its-homolog
#5
Yu Sun, Yansong Li, Xingyu Meng, Bin Qiao, Pan Hu, Xianmei Meng, Shiying Lu, Honglin Ren, Zengshan Liu, Yu Zhou
A competitive fluorescence-linked immunosorbent assay (FLISA) was developed using rhodamine B isothiocyanate (RBITC) as the model fluorescent dye conjugate monoclonal antibody (McAb) for detection of Phe and its homolog (acenaphthene, fluorene, fluoranthene, pyrene and indeno [1,2,3-cd] pyrene) in water samples. The detection range of the assay for Phe was from 2.10 to 91.95 ng/mL. The limit of detection was 1.05 ng/mL, which was approximately 2-fold lower than that of traditional ic-ELISA. Compared with traditional ic-ELISA, more than 70 min was saved because of only one immunoreaction step was needed to accomplish the assay...
February 16, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29458004/real-time-analysis-of-cleavage-and-religation-activity-of-human-topoisomerase-1-based-on-ternary-fluorescence-resonance-energy-transfer-dna-substrate
#6
Zhenxing Wang, Hui Ouyang, Cinzia Tesauro, Alessio Ottaviani, Yong He, Paola Fiorani, Hui Xie, Alessandro Desideri, Zhifeng Fu
Human topoisomerase 1B is a ubiquitous and essential enzyme involved in relaxing the topological state of supercoiled DNA to allow the progression of fundamental DNA metabolism. Its enzymatic catalytic cycle consists of cleavage and religation reaction. A ternary fluorescence resonance energy transfer biosensor based on a suicide DNA substrate conjugated with three fluorophores has been developed to monitor both cleavage and religation Topoisomerase I catalytic function. The presence of fluorophores does not alter the specificity of the enzyme catalysis on the DNA substrate...
February 16, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29458003/fgf-23-pth-and-vitamin-d-status-in-end-stage-renal-disease-patients-affected-by-vdr-foki-and-bsmi-variants
#7
Mouna Bouksila, Wajih Kaabachi, Mehdi Mrad, Wided Smaoui, Elhem Cheour El Kateb, Mohammed Karim Zouaghi, Kamel Hamzaoui, Afef Bahlous
OBJECTIVES: The aim of this study was to evaluate the association between two VDR SNPs FokI and BsmI and mineral status in ESRD patients. DESIGN AND METHODS: Our case-control study included 100 patients with chronic renal failure in ESRD and 149 healthy subjects. We measured the serum Vitamin D levels and the serum intact PTH level by Electrochemiluminescence Technology (cobas E411 analyzer). We evaluated the serum FGF23 levels by indirect ELISA method. The genotyping of two VDR gene variants FokI and BsmI was carried out by PCR-RFLP technique...
February 16, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29457974/a-multi-omics-analysis-of-glycine-max-leaves-reveals-alteration-in-flavonoid-and-isoflavonoid-metabolism-upon-ethylene-and-abscisic-acid-treatment
#8
Ravi Gupta, Cheol Woo Min, Katharina Kramer, Ganesh Kumar Agrawal, Randeep Rakwal, Ki-Hun Park, Yiming Wang, Iris Finkemeier, Sun Tae Kim
Phytohormones are central to the plant growth and development. Despite the advancement in our knowledge of hormone signaling, downstream targets and their interactions upon hormones action remain largely fragmented, especially at the protein and metabolite levels. With an aim to get new insight into the effects of two hormones, ethylene (ET) and abscisic acid (ABA), this study utilizes an integrated proteomics and metabolomics approach to investigate their individual and combined (ABA+ET) signaling in soybean leaves...
February 19, 2018: Proteomics
https://www.readbyqxmd.com/read/29457892/boronic-acid-catalyzed-regio-and-1-2-cis-stereoselective-glycosylation-of-unprotected-sugar-acceptors-via-s-n-i-type-mechanism
#9
Masamichi Tanaka, Akira Nakagawa, Nobuya Nishi, Kiyoko Iijima, Ryuichi Sawa, Daisuke Takahashi, Kazunobu Toshima
Regio- and 1,2-cis-stereoselective chemical glycosylation of unprotected glycosyl acceptors has been in great demand for the efficient synthesis of natural glycosides. However, simultaneously regulating these selectivities has been a long-standing problem in synthetic organic chemistry. In nature, glycosyl transferases catalyze regioselective 1,2-cis-glycosylations via the SN i mechanism, yet no useful chemical glycosylations based on this mechanism have been developed. In this paper, we report a highly regio- and 1,2-cis-stereoselective SN i-type glycosylation of 1,2-anhydro donors and unprotected sugar acceptors using p-nitrophenylboronic acid (10e) as a catalyst in the presence of water under mild conditions...
February 19, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29457872/simple-method-for-the-determination-of-personal-care-product-ingredients-in-lettuce-by-ultrasound-assisted-extraction-combined-with-solid-phase-microextraction-followed-by-gc-ms
#10
Jerónimo Cabrera-Peralta, Araceli Peña-Alvarez
A simple method for the simultaneous determination of personal care product ingredients: galaxolide, tonalide, oxybenzone, 4-methylbenzyliden camphor, padimate-o, 2-ethylhexyl methoxycinnamate, octocrylene, triclosan and methyl triclosan in lettuce by ultrasound-assisted extraction combined with solid-phase microextraction followed by gas chromatography with mass spectrometry was developed. Lettuce was directly extracted by ultrasound-assisted extraction with methanol, this extract was combined with water, extracted by solid-phase microextraction in immersion mode and analyzed by gas chromatography with mass spectrometry...
February 19, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29457833/a-green-method-to-determine-vuv-185-nm-fluence-rate-based-on-hydrogen-peroxide-production-in-aqueous-solution
#11
Laxiang Yang, Mengkai Li, Wentao Li, James R Bolton, Zhimin Qiang
A mini-fluidic vacuum ultraviolet/ultraviolet (VUV/UV) photoreaction system (MVPS) was developed in our previous study. Based on the MVPS, a green method to determine VUV fluence rate has been developed using the production rate of H2 O2 when water is exposed to 185 nm VUV. The H2 O2 production followed pseudo-zero-order reaction kinetics well over the first 10 min of VUV/UV exposure. This new method was well calibrated with a standard cis-cyclooctene cis-trans photoisomerization actinometer as recommended by the International Union of Pure and Applied Chemistry...
February 19, 2018: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29457812/diffusive-interaction-of-multiple-surface-nanobubbles-shrinkage-growth-and-coarsening
#12
Xiaojue Zhu, Roberto Verzicco, Xuehua Zhang, Detlef Lohse
Surface nanobubbles are nanoscopic spherical-cap shaped gaseous domains on immersed substrates which are stable, even for days. After the stability of a single surface nanobubble has been theoretically explained, i.e. contact line pinning and gas oversaturation are required to stabilize it against diffusive dissolution [Lohse and Zhang, Phys. Rev. E, 2015, 91, 031003(R)], here we focus on the collective diffusive interaction of multiple nanobubbles. For that purpose we develop a finite difference scheme for the diffusion equation with the appropriate boundary conditions and with the immersed boundary method used to represent the growing or shrinking bubbles...
February 19, 2018: Soft Matter
https://www.readbyqxmd.com/read/29457773/optical-waveguide-and-room-temperature-high-quality-nanolasers-from-tin-catalyzed-cdsse-nanostructures
#13
Pengfei Guo, Xia Shen, Baolong Zhang, Haibin Sun, Zhijun Zou, Wenchao Yang, Ke Gong, Yongsong Luo
A simple two-step CVD method is developed to realize the growth of high-quality tin-catalyzed CdSSe alloy nanowires. Microstructural characterizations demonstrate that these wires are high-quality crystalline nanostructures. Local photoluminescence investigation of these nanostructures shows a typical band edge emission at 656 nm with a full-width at half-maximum of 22.3 nm. Optical waveguide measurement along an individual nanowire indicates that the output signal of the guided light has a rapid linear decrease accompanied with maximum redshift about 109 meV after the transmission of 102 μm...
February 19, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29457722/spatially-and-temporally-resolved-detection-of-arsenic-in-a-capillary-dbd-by-hydride-generation-high-resolved-optical-emission-spectrometry
#14
Sebastian Burhenn, Jan Kratzer, Milan Svoboda, Felix David Klute, Antje Michels, Damir Veza, Joachim Franzke
A new method for arsenic detection by optical emission spectrometry (OES) is presented. Arsine (AsH3) is generated from liquid solutions by means of hydride generation (HG) and introduced into a capillary dielectric barrier discharge (DBD) where it is atomized and excited. A great challenge in OES is the reduction of the recorded background signal because it negatively affects the limit of detection (LOD). In conventional DBD-OES methods the signal intensity of the line of interest, in this case arsenic, is integrated over a long timescale...
February 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29457721/reconstructing-dynamic-promoter-activity-profiles-from-reporter-gene-data
#15
Soumya Kannan, Thomas Sams, Jérôme Maury, Christopher T Workman
Accurate characterization of promoter activity is important when designing expression systems for systems biology and metabolic engineering applications. Promoters that respond to changes in the environment enable the dynamic control of gene expression without the necessity of inducer compounds, for example. However, the dynamic nature of these processes poses challenges for estimating promoter activity. Most experimental approaches utilize reporter gene expression to estimate promoter activity. Typically the reporter gene encodes a fluorescent protein that is used to infer a constant promoter activity despite the fact that the observed output may be dynamic and is a number of steps away from the transcription process...
February 19, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29457696/reshaping-a-multimode-laser-beam-into-a-constructed-gaussian-beam-for-generating-a-thin-light-sheet
#16
Saiedeh Saghafi, Nikoo Haghi-Danaloo, Klaus Becker, Inna Sabdyusheva, Massih Foroughipour, Christian Hahn, Marko Pende, Martina Wanis, Michael Bergmann, Judith Stift, Balazs Hegedus, Balazs Dome, Hans-Ulrich Dodt
Based on the modal analysis method, we developed a model that describes the output beam of a diode pumped solid state (DPSS) laser emitting a multimode beam. Measuring the output beam profile in the near field and at the constructed far field the individual modes, their respective contributions, and their optical parameters are determined. Using this information, the beam is optically reshaped into a quasi-Gaussian beam by the interference and superposition of the various modes. This process is controlled by a mode modulator unit that includes different meso-aspheric elements and a soft-aperture...
February 19, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29457665/comparative-analysis-of-lipid-mediated-crispr-cas9-genome-editing-techniques
#17
Kelsey P Ringer, Mark G Roth, Mitchell S Garey, Ted B Piorczynski, Arminda Suli, Jason M Hansen, Jonathan K Alder
CRISPR-Cas technology has revolutionized genome engineering. While Cas9 was not the first programmable endonuclease identified, its simplicity of use has driven widespread adoption in a short period of time. While CRISPR-Cas genome editing holds enormous potential for clinical applications, its use in laboratory settings for genotype-phenotype studies and genome-wide screens has led to breakthroughs in the understanding of many molecular pathways. Numerous protocols have been described for introducing CRISPR-Cas components into cells, and here we sought to simplify and optimize a protocol for genome editing using readily available and inexpensive tools...
February 19, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29457632/rapid-eradication-of-bacterial-phytopathogens-by-atmospheric-pressure-glow-discharge-generated-in-contact-with-a-flowing-liquid-cathode
#18
Agata Motyka, Anna Dzimitrowicz, Piotr Jamroz, Ewa Lojkowska, Wojciech Sledz, Pawel Pohl
Diseases caused by phytopathogenic bacteria are responsible for significant economic losses, and these bacteria spread through diverse pathways including waterways and industrial wastes. It is therefore of interest to develop potent methods for their eradication. Here, antibacterial properties of direct current atmospheric pressure glow discharge (dc-APGD) generated in contact with flowing bacterial suspensions were examined against five species of phytopathogens. Complete eradication of Clavibacter michiganensis subsp...
February 19, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29457631/non-invasive-genetic-censusing-and-monitoring-of-primate-populations
#19
REVIEW
Mimi Arandjelovic, Linda Vigilant
Knowing the density or abundance of primate populations is essential for their conservation management and contextualizing socio-demographic and behavioral observations. When direct counts of animals are not possible, genetic analysis of non-invasive samples collected from wildlife populations allows estimates of population size with higher accuracy and precision than is possible using indirect signs. Furthermore, in contrast to traditional indirect survey methods, prolonged or periodic genetic sampling across months or years enables inference of group membership, movement, dynamics, and some kin relationships...
February 19, 2018: American Journal of Primatology
https://www.readbyqxmd.com/read/29457607/ultra-high-aspect-ratio-hybrid-materials-the-role-of-organic-guest-and-synthesis-method
#20
G V Manohara, Li Li, Andrew Whiting, H Chris Greenwell
Hybrid organic-inorganic layered double hydroxide materials have been prepared with an ultra-high aspect ratio via an environmentally friendly co-hydration approach where metal hydroxides and adamantane-carboxylic acid were used as the reagents. The method avoids use of either acidic or basic precipitation methods, or using a large excess of anion. The effect of organic anion, metal cation and synthesis method on developing ultra-high aspect ratio crystallites was studied.
February 19, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
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