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D Box

I Adamska, K Marhelava, D Walkiewicz, U Kedzierska, M Markowska, P M Majewski
Our recent research on the pineal gland of young chickens confirmed that three genes encoding enzymes involved in pineal melatonin biosynthesis, tryptophan hydroxylase 1 (Tph1), arylalkylamine-N-acetyltransferase (Aanat) and acetylserotonin O-methyltransferase (Asmt), are transcribed rhythmically under light:dark (L:D) 12:12 conditions in vivo. Additionally, in the pineal gland of maturing chickens, the dopa decarboxylase (Ddc) gene is transcribed rhythmically at a specific stage of the developmental process...
August 2016: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
U Thomas Meier
Aside from nucleoli, Cajal bodies (CBs) are the best-characterized organelles of mammalian cell nuclei. Like nucleoli, CBs concentrate ribonucleoproteins (RNPs), in particular, spliceosomal small nuclear RNPs (snRNPs) and small nucleolar RNPs (snoRNPs). In one of the best-defined functions of CBs, most of the snoRNPs are involved in site-specific modification of snRNAs. The two major modifications are pseudouridylation and 2'-O-methylation that are guided by the box H/ACA and C/D snoRNPs, respectively. This review details the modifications, their function, the mechanism of modification, and the machineries involved...
October 24, 2016: RNA Biology
O Assadian, D J Leaper, A Kramer, K J Ousey
BACKGROUND: Few studies have explored the microbial contamination of glove boxes in clinical settings. The objective of this observational study was to investigate whether a new glove packaging system in which single gloves are dispensed vertically, cuff end first, has lower levels of contamination on the gloves and on the surface around the box aperture compared with conventional glove boxes. METHODS: Seven participating sites were provided with vertical glove dispensing systems (modified boxes) and conventional boxes...
September 15, 2016: Journal of Hospital Infection
Shirin Babaee, Ali Daneshfar, Tahere Khezeli
Magnetically separable layered double hydroxide Ni/CuAl-LDH nanocomposites were synthesized and employed as ultrasonic-assisted dispersive micro-solid phase extraction (UA-D-μSPE) sorbent to extract several carboxylic acids (namely propionic, butyric, pentanoic, hexanoic, heptanoic, octanoic, and decanoic) from non-alcoholic beer samples. Ni/CuAl-LDH sorbent was characterized by Fourier transform-infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), and vibrating sample magnetometry (VSM). Effective variables such as amount of sorbent (mg), pH and ionic strength of sample solution, volume of eluent solvent (μL), vortex, and ultrasonic times (min) were investigated via fractional factorial design (FFD)...
January 2017: Ultrasonics Sonochemistry
Cinthia D Coria-Lucero, Rebeca S Golini, Ivana T Ponce, Nicolas Deyurka, Ana C Anzulovich, Silvia M Delgado, Lorena S Navigatore-Fonzo
Aging brain undergoes several changes leading to a decline in cognitive functions. Memory and learning-related genes such as Creb, Bdnf and its receptor TrkB, are expressed in different brain regions including prefrontal cortex. Those genes' proteins regulate a wide range of functions such as synaptic plasticity and long-term potentiation. In this work, our objectives were: 1) to investigate whether Creb1, Bdnf and TrkB genes display endogenous circadian expression rhythms, in the prefrontal cortex of rats maintained under constant darkness conditions; 2) to study the synchronization of those temporal patterns to the local cellular clock and 3) to evaluate the aging consequences on both cognition-related genes and activating clock transcription factor, BMAL1, rhythms...
October 19, 2016: Brain Research
Francesca Palone, Roberta Vitali, Salvatore Cucchiara, Maurizio Mennini, Alessandro Armuzzi, Daniela Pugliese, Renata DʼIncà, Brigida Barberio, Laura Stronati
BACKGROUND: Fecal high mobility group box 1 (HMGB1) has been suggested to be a novel noninvasive biomarker of gut inflammation. We aimed to assess the reliability of fecal HMGB1, compared with fecal calprotectin (FC), in detecting intestinal inflammation in pediatric and adult patients with inflammatory bowel disease (IBD) and to evaluate the accuracy of HMGB1 in identifying patients with IBD in clinical and endoscopic remission who still have histologic features of inflammation. METHODS: Stool samples from 85 children with IBD (49 Crohn's disease [CD] and 36 ulcerative colitis [UC] and 119 adults [57 Crohn's disease and 62 ulcerative colitis]) were analyzed for the study...
October 13, 2016: Inflammatory Bowel Diseases
Seong-Jin Kim, Tae Hyeong Lee, Sang Hee Nam, Ji-Hong Kim, Sangho Oh, Yeon Sook Cho, Myeong Sup Lee, Sehoon Choi, Peter C W Lee
BACKGROUND: The UBA6-specific E2 conjugating enzyme 1 (USE1) ubiquitin enzyme cascade is a poorly characterized arm of the ubiquitin-proteasome system. We investigated whether the UBA6-USE1 enzyme cascade plays a role in lung cancer tumorigenesis. METHODS: USE1 expression was assessed in tumor-normal paired samples from 106 lung cancer patients by immunoblot. USE1 was stably overexpressed and knocked down in lung cancer cell lines to evaluate cell proliferation, colony formation, and invasion...
March 2017: Journal of the National Cancer Institute
Grigory A Stepanov, Julia A Filippova, Anna A Nushtaeva, Elena V Kuligina, Olga A Koval, Vladimir A Richter, Dmitriy V Semenov
Fragments of small nucleolar RNAs (snoRNAs) were found among various non-coding RNAs (ncRNAs) circulating in human blood. Currently, the function of such cell-free sno-derived-RNAs is not clearly defined. This work is aimed at identifying regulatory pathways controlled by extracellular snoRNAs. In order to determine the molecular targets and pathways affected by artificial snoRNAs, we performed Illumina array analysis of MCF-7 human adenocarcinoma cells transfected with box C/D RNAs. The genes related to the innate immune response and apoptotic cascades were found to be activated in transfected cells compared with control cells...
2016: Advances in Experimental Medicine and Biology
Vanessa Tripp, Roman Martin, Alvaro Orell, Omer S Alkhnbashi, Rolf Backofen, Lennart Randau
Archaeal and eukaryotic organisms contain sets of C/D box s(no)RNAs with guide sequences that determine ribose 2'-O-methylation sites of target RNAs. The composition of these C/D box sRNA sets is highly variable between organisms and results in varying RNA modification patterns which are important for ribosomal RNA folding and stability. Little is known about the genomic organization of C/D box sRNA genes in archaea. Here, we aimed to obtain first insights into the biogenesis of these archaeal C/D box sRNAs and analyzed the genetic context of more than 300 archaeal sRNA genes...
October 14, 2016: Molecular Microbiology
M A Landrum, N A Cox, D E Cosby, M E Berrang, S M Russell
New regulations and performance standards for Campylobacter have been implemented by the USDA - Food Safety and Inspection Service (FSIS). The objective of this study was to evaluate treatment with a low pH processing aid (CMS(1) PoultrypHresh), a formulated low pH processing aid, to reduce numbers of Campylobacter which could help companies meet regulatory requirements. Two experiments (3 replicates each) were conducted. Experiment 1, in each of 3 replicates, skin-on split chicken breasts (n = 15) were obtained from a local grocery and divided into groups of 5...
October 12, 2016: Poultry Science
Thomas E Kraft, Monique R Heitmeier, Marina Putanko, Rachel L Edwards, Ma Xenia G Ilagan, Maria A Payne, Joseph M Autry, David D Thomas, Audrey R Odom, Paul W Hruz
The glucose transporter PfHT is essential to the survival of the malaria parasite Plasmodium falciparum and has been shown to be a druggable target with high potential for pharmacological intervention. Identification of compounds against novel drug targets is crucial to combating resistance against current therapeutics. Here, we describe the development of a cell-based assay system readily adaptable to high-throughput screening that directly measures compound effects on PfHT-mediated glucose transport. Intracellular glucose concentrations are detected using a genetically encoded fluorescence resonance energy transfer (FRET)-based glucose-sensor...
October 10, 2016: Antimicrobial Agents and Chemotherapy
Tao Tian, Weiliang Tian, Fan Yang, Risheng Zhao, Qian Huang, Yunzhao Zhao
BACKGROUND: Sphingosine kinase 1 (SphK1)/sphingosine-1-phosphate (S1P)/sphingosine-1-phosphate receptors (S1PRs) signaling plays a key role in inflammatory responses. Lei et al. showed that SphK1 inhibition presented a hepatoprotective effect on acute liver damage via decreasing hepatic high-mobility group box 1 (HMGB1) cytoplasmic translocation. OBJECTIVE: We aim to determine whether SphK1 or S1PRs inhibition improves lipopolysaccharide (LPS)/D-galactosamine (GalN)-induced acute liver failure by inhibiting the mitogen-activated protein kinases (MAPKs) pathway...
October 2016: United European Gastroenterology Journal
Mohammad J Alhilaly, Megalamane S Bootharaju, Chakra P Joshi, Tabot M D Besong, Abdul-Hamid Emwas, Rosalba Juarez Mosqueda, Sami Kaappa, Sami Malola, Karim Adil, Aleksander Shkurenko, Hannu Häkkinen, Mohamed Eddaoudi, Osman M Bakr
Engineering the surface ligands of metal nanoparticles is critical in designing unique arrangements of metal atoms. Here, we report the synthesis and total structure determination of a large box-shaped Ag67 nanocluster (NC) protected by a mixed shell of thiolate (2,4-dimethylbenzenethiolate, SPhMe2) and phosphine (triphenylphosphine, PPh3) ligands. Single crystal X-ray diffraction (SCXRD) and electrospray ionization mass spectrometry (ESI-MS) revealed the cluster formula to be [Ag67(SPhMe2)32(PPh3)8]3+. The crystal structure shows an Ag23 metal core covered by a layer of Ag44S32P8 arranged in the shape of a box...
October 13, 2016: Journal of the American Chemical Society
Christoph Partes, Can Yildirim, Swen Schuster, Martin Kind, Jan W Bats, Michael Zharnikov, Andreas Terfort
A series of three homologous arene[2,3-d]-oxazole-2-thiols (benzoxazole-2-thiol (BOxSH), naphthaleneoxazole-2-thiol (NOxSH), and anthraceneoxazoline-2-thiol (AOxSH)) were deposited onto Au(111) to obtain surfaces suitable as injection layers for organic electronics. The guiding idea was that the increasingly extended conjugated system would lower the band gap of the films while the introduction of the annellated heteroaromatic ring would provide the opportunity for pseudo-symmetric attachment of the sulfur anchor, what should lower the conformational freedom of the system...
October 11, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
Jahanshah Ashkani, D J G Rees
Specificity in the GSI response results from the S-haplotype-specific molecular interaction of S-locus F-box (SLF/SFB) and SRNase proteins in the self-incompatibility locus (S-locus). The answer to the question of how these two components of the S-locus (SRNase and SLF/SFB) interact has been gathered from several models. Since there is not enough evidence as to which one is the definitive model, none of them can be ruled out. Despite the identification of interacting protein elements, the mechanism by which SLF/SFB and SRNase interact to differently trigger the self-incompatibility among families and subfamilies remain uncertain...
October 10, 2016: Scientific Reports
Liliya V Vasileva, Damianka P Getova, Nina D Doncheva, Andrey S Marchev, Milen I Georgiev
: Rhodiola rosea L., family Crassulaceae also known as Golden Root or Arctic root is one of the most widely used medicinal plants with effect on cognitive dysfunction, psychological stress and depression. The aim of the study was to examine the effect of a standardized commercial Rhodiola extract on learning and memory processes in naive rats as well as its effects in rats with scopolamine-induced memory impairment. MATERIALS AND METHODS: Sixty male Wistar rats were used in the study...
October 5, 2016: Journal of Ethnopharmacology
Peter Løvendahl, Lars Peter Sørensen, Martin Bjerring, Jan Lassen
Dairy cows milked in automatic milking systems (AMS) with more than 1 milking box may, as individuals, have a preference for specific milking boxes if allowed free choice. Estimates of quantitative genetic variation in behavioral traits of farmed animals have previously been reported, with estimates of heritability ranging widely. However, for the consistency of choice in dairy cows, almost no published estimates of heritability exist. The hypothesis for this study was that choice consistency is partly under additive genetic control and partly controlled by permanent environmental (animal) effects...
October 5, 2016: Journal of Dairy Science
Z Wang, D B Zhang, W Zhou
Objective: To evaluate the expression of transcription factor T-bet (T-box expressed in T cells) and explore its effects on prognosis in peripheral T-cell lymphoma (PTCL). Methods: This study included 109 previously untreated patients diagnosed with PTCL in the pathology system at Peking Union Medical College Hospital from 2007 to 2013. Formalin-fixed, paraffin-embedded blocks and clinical information of these patients were collected. T-bet expression was examined by immunohistochemical stain, while its relationship with clinical feature and prognosis was analyzed...
September 14, 2016: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
Manuel Nieto-Domínguez, Alicia Prieto, Beatriz Fernández de Toro, Francisco Javier Cañada, Jorge Barriuso, Zach Armstrong, Stephen G Withers, Laura I de Eugenio, María Jesús Martínez
BACKGROUND: Glycosides are compounds displaying crucial biological roles and plenty of applications. Traditionally, these molecules have been chemically obtained, but its efficient production is limited by the lack of regio- and stereo-selectivity of the chemical synthesis. As an interesting alternative, glycosidases are able to catalyze the formation of glycosides in a process considered green and highly selective. In this study, we report the expression and characterization of a fungal β-xylosidase in Pichia pastoris...
October 4, 2016: Microbial Cell Factories
Séverine Massenet, Edouard Bertrand, Céline Verheggen
Box C/D and box H/ACA snoRNAs are abundant non-coding RNAs that localize in the nucleolus and mostly function as guides for nucleotide modifications. While a large pool of snoRNAs modifies ribosomal RNAs, an increasing number of snoRNAs could also potentially target mRNAs. ScaRNAs belong to a family of specific RNAs that localize in Cajal bodies and that are structurally similar to snoRNAs. Most scaRNAs are involved in snRNA modification, while telomerase RNA, which contains H/ACA motifs, functions in telomeric DNA synthesis...
October 7, 2016: RNA Biology
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