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https://www.readbyqxmd.com/read/28449431/three-dimensional-printing-of-external-airway-splints-for-tracheomalacia
#1
EDITORIAL
Hester F Shieh, Russell W Jennings
No abstract text is available yet for this article.
March 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28449406/a-water-stable-dual-channel-luminescence-sensor-for-uo22-ion-based-on-an-anionic-terbium-iii-metal-organic-framework
#2
Junwei Ye, Raji Feyisa Bogale, Yangwei Shi, Yanzhen Chen, Xigang Liu, Yaoyao Yang, Siqi Zhang, Jianzhang Zhao, Guiling Ning
A stable 3D Tb(III)-based metal-organic framework [Tb(BPDC)2]*(CH3)2NH2 (DUT-101) was synthesized, and it is the first efficient dual-channel luminescence sensor for aqueous UO22+ ion. DUT-101 possesses an anionic three-dimensional framework and protonated dimethylamine molecules embedded within the channels. Intense green emission of DUT-101 could be highly selectively and sensitively quenched by UO22+ ions even in the coexistence of other competing metal ions. The possible sensing mechanism was proposed as well based on both suppression of luminescence resonance energy transfer and enhancement of intermolecular electronic transfer...
April 27, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28449356/fetal-head-circumference-and-subpubic-angle-are-independent-risk-factors-for-unplanned-cesarean-and-operative-delivery
#3
Giuseppe Rizzo, Elisa Aiello, Costanza Bosi, Francesco D' Antonio, Domenico Arduini
INTRODUCTION: To ascertain whether combined ultrasound assessment of fetal head circumference (HC) and maternal subpubic angle (SPA) prior to the onset of labour may predict the likelihood of an unplanned operative delivery (UOD) in nulliparous women at term. MATERIAL AND METHODS: Prospective cohort study of singleton pregnancies in cephalic presentation. Pregnancies experiencing UOD secondary to fetal distress were excluded. HC was assessed transabdominally while SPA values were obtained from a reconstructed coronal plane on three-dimensional(3D) ultrasound performed trans-labially at 36-38 weeks of gestation...
April 27, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28449078/surface-electrocardiography-characteristics-and-radiofrequency-catheter-ablation-of-idiopathic-ventricular-arrhythmias-originating-from-the-left-infero-septal-papillary-muscles-differences-from-those-originating-from-the-left-posterior-fascicle
#4
Shixing Li, Zulu Wang, Zhaoliang Shan, Xiangmin Shi, Ming Liang, Yanchun Liang, Zhiqing Jin, Yaling Han, Yutang Wang
Aims: Distinguishing between ventricular arrhythmias originating from the left ventricular infero-septal papillary muscles (PM) and those from the left posterior fascicle (LPF) by surface electrocardiography (ECG) is very difficult. This study aimed to report the ECG characteristics and radiofrequency catheter ablation of PM and LPF ventricular arrhythmias. Methods and results: A total of 127 patients underwent catheter ablation of idiopathic ventricular arrhythmias originating from the LPF (n = 106; 85 males; 10-70 years) or PM (n = 21; 14 males; 4-68 years) were studied...
April 24, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28449068/development-of-a-novel-human-recellularized-endometrium-that-responds-to-a-28-day-hormone-treatment
#5
Susan A Olalekan, Joanna E Burdette, Spiro Getsios, Teresa K Woodruff, J Julie Kim
Three dimensional (3D) in vitro models have been established to study the physiology and pathophysiology of the endometrium. With emerging evidence that the native extracellular matrix (ECM) provides appropriate cues and growth factors essential for tissue homeostasis, we describe, a novel 3D endometrium in vitro model developed from decellularized human endometrial tissue repopulated with primary endometrial cells. Analysis of the decellularized endometrium using mass spectrometry analysis revealed an enrichment of cell adhesion molecules, cytoskeletal proteins and ECM proteins such as Collagen IV and Laminin...
April 25, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28448922/affine-and-non-affine-deformations-quantified-in-cytoskeletal-networks-through-three-dimensional-form-finding-model
#6
Yifan Wang, Jinghai Gong, Denis Wirtz, Benjamin W Schafer
Actin filaments and cross-linkers are main components of cytoskeletal networks in eukaryotic cells, and they support bending moments and axial forces respectively. A three-dimensional form-finding model is proposed in this work to investigate affine and non-affine deformations in cytoskeletal networks. In recent studies, modeling of cytoskeletal networks turns out to be a key piece in the cell mechanics puzzle. We used form-finding analysis to compute and analyze cytoskeletal models. A three-dimensional model is much more flexible and contains more elements than a two-dimensional model, and non-linear finite element analysis is difficult to converge...
April 20, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28448851/accurate-3d-reconstruction-of-bony-surfaces-using-ultrasonic-synthetic-aperture-techniques-for-robotic-knee-arthroplasty
#7
William Kerr, Philip Rowe, Stephen Gareth Pierce
Robotically guided knee arthroplasty systems generally require an individualized, preoperative 3D model of the knee joint. This is typically measured using Computed Tomography (CT) which provides the required accuracy for preoperative surgical intervention planning. Ultrasound imaging presents an attractive alternative to CT, allowing for reductions in cost and the elimination of doses of ionizing radiation, whilst maintaining the accuracy of the 3D model reconstruction of the joint. Traditional phased array ultrasound imaging methods, however, are susceptible to poor resolution and signal to noise ratios (SNR)...
April 19, 2017: Computerized Medical Imaging and Graphics: the Official Journal of the Computerized Medical Imaging Society
https://www.readbyqxmd.com/read/28448819/construction-of-three-dimensional-vascularized-functional-human-liver-tissue-using-a-layer-by-layer-cell-coating-technique
#8
Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yasunari Fukuda, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Hiroshi Wada, Kunihito Gotoh, Koichi Kawamoto, Yuichiro Doki, Masaki Mori, Mitsuru Akashi
The creation of artificial liver tissue is an active area of research due to the shortage of donors for liver transplantation. Here we investigated whether a simple and efficient cell coating technique developed in our laboratory could be used to generate functional vascularized liver tissue. This technique creates three-dimensional tissue by loading cells sterically onto other cells that have been coated with layer-by-layer (LbL) nanofilms of fibronectin and gelatin, two extracellular matrix proteins. We used this technique to construct homogenous, dense, well-vascularized liver tissue from cryopreserved human primary hepatocytes, human umbilical vein endothelial cells, and normal human dermal fibroblasts...
February 28, 2017: Biomaterials
https://www.readbyqxmd.com/read/28448629/structure-and-function-of-a-novel-gh8-endoglucanase-from-the-bacterial-cellulose-synthase-complex-of-raoultella-ornithinolytica
#9
Sandra Mara Naressi Scapin, Flavio Henrique Moreira Souza, Leticia Maria Zanphorlin, Thamyres Silva de Almeida, Youssef Bacila Sade, Alexander Machado Cardoso, Guilherme Luiz Pinheiro, Mario Tyago Murakami
Cellulose synthesis in bacteria is a complex process involving the concerted action of several enzymes whose genes are often organized in operons. This process influences many fundamental physiological aspects such as bacteria and host interaction, biofilm formation, among others. Although it might sound contradictory, the participation of cellulose-degrading enzymes is critical to this process. The presence of endoglucanases from family 8 of glycosyl hydrolases (GH8) in bacterial cellulose synthase (Bcs) complex has been described in different bacteria, including the model organism Komagataeibacter xylinus; however, their role in this process is not completely understood...
2017: PloS One
https://www.readbyqxmd.com/read/28448235/intracranial-artery-steno-occlusion-diagnosis-by-using-three-dimensional-parallel-multichannel-transmission-mr-imaging
#10
Mi Sun Chung, Seung Chai Jung, Seon-Ok Kim, Ho Sung Kim, Choong Gon Choi, Sang Joon Kim, Sun U Kwon, Dong-Wha Kang, Jong S Kim
Purpose To determine whether magnetic resonance (MR) imaging by using two-dimensional spatially selective radiofrequency (RF) excitation pulses (zoomed MR imaging) is a reliable method for the diagnosis of intracranial artery steno-occlusion compared with full-field-of-view (FOV) MR imaging. Materials and Methods The institutional review board approved this retrospective study and informed consent was waived. From December 2014 to August 2015, 88 patients who underwent both full-FOV MR imaging and zoomed MR imaging for further evaluation of intracranial artery steno-occlusion at prior luminal evaluation were included...
April 27, 2017: Radiology
https://www.readbyqxmd.com/read/28448112/real-time-imaging-of-2d-and-3d-temperature-distribution-coating-of-metal-ion-intercalated-organic-layered-composites-with-tunable-stimuli-responsive-properties
#11
Machi Takeuchi, Hiroaki Imai, Yuya Oaki
Organic layered materials have intercalation and dynamic properties. The dynamic properties are tuned by the intercalation of the guests. In general, howevver, it is not easy to achieve homogeneous and thin coating of the layered materials on substrates with complex shapes because of the two-dimentional anisotropic structures. In the present work, the layered organic composites were homogeneously coated on a variety of substrates for application to practical devices. The metal-ion-intercalated layered polydiacetylene (PDA-Mn+) with tunable stimuli-responsive color-change properties was coated on substrates, such as paper and cotton consisting of cellulose fibers...
April 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28448053/automated-contraction-analysis-of-human-engineered-heart-tissue-for-cardiac-drug-safety-screening
#12
Ingra Mannhardt, Umber Saleem, Anika Benzin, Thomas Schulze, Birgit Klampe, Thomas Eschenhagen, Arne Hansen
Cardiac tissue engineering describes techniques to constitute three dimensional force-generating engineered tissues. For the implementation of these procedures in basic research and preclinical drug development, it is important to develop protocols for automated generation and analysis under standardized conditions. Here, we present a technique to generate engineered heart tissue (EHT) from cardiomyocytes of different species (rat, mouse, human). The technique relies on the assembly of a fibrin-gel containing dissociated cardiomyocytes between elastic polydimethylsiloxane (PDMS) posts in a 24-well format...
April 15, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28448052/an-in-vitro-organ-culture-model-of-the-murine-intervertebral-disc
#13
Jennifer W Liu, Kevin H Lin, Christian Weber, Sameer Bhalla, Sean Kelso, Kaixi Wang, Simon Y Tang
Intervertebral disc (IVD) degeneration is a significant contributor to low back pain. The IVD is a fibrocartilaginous joint that serves to transmit and dampen loads in the spine. The IVD consists of a proteoglycan-rich nucleus pulposus (NP) and a collagen-rich annulus fibrosis (AF) sandwiched by cartilaginous end-plates. Together with the adjacent vertebrae, the vertebrae-IVD structure forms a functional spine unit (FSU). These microstructures contain unique cell types as well as unique extracellular matrices...
April 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28448015/quantification-of-strain-in-a-porcine-model-of-skin-expansion-using-multi-view-stereo-and-isogeometric-kinematics
#14
Adrian Buganza Tepole, Elbert E Vaca, Chad A Purnell, Michael Gart, Jennifer McGrath, Ellen Kuhl, Arun K Gosain
Tissue expansion is a popular technique in plastic and reconstructive surgery that grows skin in vivo for correction of large defects such as burns and giant congenital nevi. Despite its widespread use, planning and executing an expansion protocol is challenging due to the difficulty in measuring the deformation imposed at each inflation step and over the length of the procedure. Quantifying the deformation fields is crucial, as the distribution of stretch over time determines the rate and amount of skin grown at the end of the treatment...
April 16, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28448014/an-efficient-and-flexible-cell-aggregation-method-for-3d-spheroid-production
#15
Sarah M Maritan, Eric Y Lian, Lois M Mulligan
Monolayer cell culture does not adequately model the in vivo behavior of tissues, which involves complex cell-cell and cell-matrix interactions. Three-dimensional (3D) cell culture techniques are a recent innovation developed to address the shortcomings of adherent cell culture. While several techniques for generating tissue analogues in vitro have been developed, these methods are frequently complex, expensive to establish, require specialized equipment, and are generally limited by compatibility with only certain cell types...
March 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28448013/precision-cut-mouse-lung-slices-to-visualize-live-pulmonary-dendritic-cells
#16
Miranda R Lyons-Cohen, Seddon Y Thomas, Donald N Cook, Hideki Nakano
Inhalation of allergens and pathogens elicits multiple changes in a variety of immune cell types in the lung. Flow cytometry is a powerful technique for quantitative analysis of cell surface proteins on immune cells, but it provides no information on the localization and migration patterns of these cells within the lung. Similarly, chemotaxis assays can be performed to study the potential of cells to respond to chemotactic factors in vitro, but these assays do not reproduce the complex environment of the intact lung...
April 5, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447989/fabrication-of-extracellular-matrix-derived-foams-and-microcarriers-as-tissue-specific-cell-culture-and-delivery-platforms
#17
Anna Kornmuller, Cody F C Brown, Claire Yu, Lauren E Flynn
Cell function is mediated by interactions with the extracellular matrix (ECM), which has complex tissue-specific composition and architecture. The focus of this article is on the methods for fabricating ECM-derived porous foams and microcarriers for use as biologically-relevant substrates in advanced 3D in vitro cell culture models or as pro-regenerative scaffolds and cell delivery systems for tissue engineering and regenerative medicine. Using decellularized tissues or purified insoluble collagen as a starting material, the techniques can be applied to synthesize a broad array of tissue-specific bioscaffolds with customizable geometries...
April 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447974/3d-whole-heart-myocardial-tissue-analysis
#18
Hans Thijs Van den Broek, Leon De Jong, Pieter A Doevendans, Steven A J Chamuleau, Frebus J Van Slochteren, René Van Es
Cardiac regenerative therapies aim to protect and repair the injured heart in patients with ischemic heart disease. By injecting stem cells or other biologicals that enhance angio- or vasculogenesis into the infarct border zone (IBZ), tissue perfusion is improved, and the myocardium can be protected from further damage. For maximum therapeutic effect, it is hypothesized that the regenerative substance is best delivered to the IBZ. This requires accurate injections and has led to the development of new injection techniques...
April 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447928/evaluation-of-three-dimensional-versus-conventional-laparoscopy-for-kidney-transplant-procedures-in-a-human-cadaveric-model
#19
Bulang He, Lingjun Mou, Ronald De Roo, Gabrielle C Musk, Jeffrey M Hamdorf
OBJECTIVES: There are increased reports that kidney transplant can be performed by laparoscopic surgery. The further development of this technique could revolutionize human kidney transplant surgery. However, laparoscopic kidney transplant demands a high level of skill for vascular anastomoses. The emerging technology of the three-dimensional, high-definition laparoscopic system may facilitate the application of this technique. Therefore, in this study, we evaluated this system in performing kidney transplant surgery versus the two-dimensional laparoscopic system...
April 27, 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28447612/distinct-molecular-cues-ensure-a-robust-microtubule-dependent-nuclear-positioning-in-the-drosophila-oocyte
#20
Nicolas Tissot, Jean-Antoine Lepesant, Fred Bernard, Kevin Legent, Floris Bosveld, Charlotte Martin, Orestis Faklaris, Yohanns Bellaïche, Maïté Coppey, Antoine Guichet
Controlling nucleus localization is crucial for a variety of cellular functions. In the Drosophila oocyte, nuclear asymmetric positioning is essential for the reorganization of the microtubule (MT) network that controls the polarized transport of axis determinants. A combination of quantitative three-dimensional live imaging and laser ablation-mediated force analysis reveal that nuclear positioning is ensured with an unexpected level of robustness. We show that the nucleus is pushed to the oocyte antero-dorsal cortex by MTs and that its migration can proceed through distinct tracks...
April 27, 2017: Nature Communications
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