keyword
MENU ▼
Read by QxMD icon Read
search

Alpha-1-acid glycoprotein

keyword
https://www.readbyqxmd.com/read/27899485/circulating-microrna-122-is-associated-with-the-risk-of-new-onset-metabolic-syndrome-and-type-2-diabetes
#1
Peter Willeit, Philipp Skroblin, Alexander R Moschen, Xiaoke Yin, Dorothee Kaudewitz, Anna Zampetaki, Temo Barwari, Meredith Whitehead, Cristina M Ramírez, Leigh Goedeke, Noemi Rotllan, Enzo Bonora, Alun D Hughes, Peter Santer, Carlos Fernández-Hernando, Herbert Tilg, Johann Willeit, Stefan Kiechl, Manuel Mayr
MicroRNA-122 (miR-122) is abundant in the liver and involved in lipid homeostasis, but its relevance to the long-term risk of developing metabolic disorders is unknown. We therefore measured circulating miR-122 in the prospective population-based Bruneck Study (n=810; survey year: 1995). Circulating miR-122 was associated with prevalent insulin resistance, obesity, metabolic syndrome, type-2 diabetes, and an adverse lipid profile. Among 92 plasma proteins and 135 lipid subspecies quantified with mass spectrometry, it correlated inversely with zinc-alpha-2-glycoprotein and positively with afamin, complement-factor H, VLDL-associated apolipoproteins, and lipid subspecies containing monounsaturated and saturated fatty acids...
November 29, 2016: Diabetes
https://www.readbyqxmd.com/read/27896921/effect-of-iron-supplementation-during-lactation-on-maternal-iron-status-and-oxidative-stress-a-randomized-controlled-trial
#2
Josh M Jorgensen, Zhenyu Yang, Bo Lönnerdal, Caroline J Chantry, Kathryn G Dewey
We examined the effect of iron-containing prenatal vitamin-mineral supplements taken postpartum on biomarkers of iron status and oxidative stress. Lactating women (n = 114) were randomly assigned to consume daily one iron-free prenatal vitamin-mineral supplement plus either 27 mg of iron or placebo for approximately 3.5 months. The placebo group took the tablets between meals, while those given iron took the tablets either with (Fe-W) or between meals (Fe-B). Blood and urine samples were collected before and after the supplementation period to analyze hemoglobin (Hb), ferritin, hepcidin, transferrin saturation (TfSat), total plasma iron, and biomarkers of oxidative stress (isoprostane and 8-hydroxy-2-deoxyguanosine (8-OHdG)) and inflammation (C-reactive protein (CRP) and alpha-1-acid glycoprotein (AGP))...
November 29, 2016: Maternal & Child Nutrition
https://www.readbyqxmd.com/read/27875723/orosomucoid-as-prognosis-factor-associated-with-inflammation-in-acute-or-nutritional-status-in-chronic-heart-failure
#3
Rosa M Agra, Alfonso Varela-Román, Rocío González-Ferreiro, Juan E Viñuela, Ana Castro-Pais, Ángel Fernández-Trasancos, Esther Díaz-Rodríguez, Ezequiel Álvarez, Marcos C Carreira, Felipe F Casanueva, José R González-Juanatey, Sonia Eiras
BACKGROUND: Inflammation and nutritional state are involved in the pathogenesis of heart failure (HF). OBJECTIVE: To study the contribution of alpha-1-acid-glycoprotein (AGP) to these factors and its prognostic value in acute (AHF) or chronic HF (CHF). METHODS: The observational study has included 147 patients (mean age 70years, 62% men) admitted to a cardiology department for HF and followed-up for an average 326.6±140.8days. Blood AGP values were measured by Enzyme-Linked ImmunoSorbent Assay...
November 9, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27760464/characterization-of-site-specific-n-glycopeptide-isoforms-of-alpha-1-acid-glycoprotein-from-an-interlaboratory-study-using-lc-ms-ms
#4
Ju Yeon Lee, Hyun Kyoung Lee, Gun Wook Park, Heeyoun Hwang, Hoi Keun Jeong, Ki Na Yun, Eun Sun Ji, Kwang Hoe Kim, Jun Seok Kim, Jong Won Kim, Sung Ho Yun, Chi-Won Choi, Seung Il Kim, Jong-Sun Lim, Seul-Ki Jeong, Young-Ki Paik, Soo-Youn Lee, Jisook Park, Su Yeon Kim, Young-Jin Choi, Yong-In Kim, Jawon Seo, Je-Yoel Cho, Myoung Jin Oh, Nari Seo, Hyun Joo An, Jin Young Kim, Jong Shin Yoo
Glycoprotein conformations are complex and heterogeneous. Currently, site-specific characterization of glycopeptides is a challenge. Herein, we sought to establish an efficient method of N-glycoprotein characterization using mass spectrometry. Using alpha-1-acid glycoprotein (AGP) as a model N-glycoprotein, its tryptic N-glycopeptides were identified, and the reproducibility of the data was examined in seven laboratories running different liquid chromatography-mass tandem spectrometry (LC-MS/MS) platforms. We used three test samples and one blind sample to evaluate instrument performance with entire sample preparation workflow...
October 19, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27743359/the-use-of-multiple-reaction-monitoring-on-qqq-ms-for-the-analysis-of-protein-and-site-specific-glycosylation-patterns-in-serum
#5
L Renee Ruhaak
In recent years, high-throughput glycomics approaches have been developed and applied to either complete biofluids, cell lysates or tissues, or proteins isolated thereof. However, during such analyses the N-glycan are released from the protein backbone and therefore site- and protein-specific information is lost. There exists a need for high-throughput methods that allow quantification of site- and protein-specific glycosylation patterns from complex biological mixtures. We here describe the use of a multiple reaction monitoring mass spectrometry based method for the generation of glycopeptide profiles of the nine high abundance glycoproteins IgG, IgA, IgM, haptoglobin, alpha-1-antitrypsin, alpha-2-macroglobulin, alpha-1-acid glycoprotein, transferrin, and complement C3...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27741252/up-regulation-of-antioxidant-proteins-in-the-plasma-proteome-during-saturation-diving-unique-coincidence-under-hypobaric-hypoxia
#6
Hideharu Domoto, Keiichi Iwaya, Fumitaka Ikomi, Hirotaka Matsuo, Yutaka Tadano, Shigenori Fujii, Kazuyoshi Tachi, Yoshiyuki Itoh, Michiya Sato, Kimitoshi Inoue, Nariyoshi Shinomiya
Saturation diving (SD) is one of the safest techniques for tolerating hyperbaric conditions for long durations. However, the changes in the human plasma protein profile that occur during SD are unknown. To identify differential protein expression during or after SD, 65 blood samples from 15 healthy Japanese men trained in SD were analyzed by two-dimensional fluorescence difference gel electrophoresis. The expression of two proteins, one 32.4 kDa with an isoelectric point (pI) of 5.8 and the other 44.8 kDa with pI 4...
2016: PloS One
https://www.readbyqxmd.com/read/27711093/in-the-search-of-novel-urine-biomarkers-for-the-early-diagnosis-of-prostate-cancer-intracellular-or-secreted-proteins-as-the-target-group-where-and-how-to-search-for-possible-biomarkers-useful-in-the-everyday-clinical-practice
#7
Amalia Katafigioti, Ioannis Katafigiotis, Stavros Sfoungaristos, Christos Alamanis, Konstantinos Stravodimos, Ioannis Anastasiou, Eleni Roumelioti, Mordechai Duvdevani, Constantinos Constantinides
OBJECTIVE: To search which category of proteins can be detected in urine in order to examine subsequently its ability to improve our accuracy for the diagnosis of Prostate Cancer (PCa) as biomarkers in clinical useful fluids like urine and serum. Material and method(s): Urine samples of 127 patients were obtained after a vigorous transrectal prostatic massage to both lobes. The patients were considered to have a high risk for PCa according to their PSA (> 4 ng/ml), their digital rectal examination (DRE) (positive for suspicious prostatic lesions) or to their abnormal PSA kinetics (PSA velocity (PSAV > 0...
October 5, 2016: Archivio Italiano di Urologia, Andrologia
https://www.readbyqxmd.com/read/27664801/is-the-unbound-concentration-of-atazanavir-of-interest-in-therapeutic-drug-monitoring
#8
David Metsu, Patrick Seraissol, Pierre Delobel, Christel Cinq-Frais, Lise Cuzin, Jacques Izopet, Etienne Chatelut, Peggy Gandia
To date, therapeutic drug monitoring (TDM) is carried out with anti-retrovirals and is usually based on total concentrations (Ct). However, for some patients, TDM does not reflect efficacy or the avoidance of toxicity as is the case for atazanavir (ATV), a HIV protease inhibitor. As the unbound concentration (Cu) is the pharmacological active form, the aim of the study was to evaluate the value of Cu and the unbound fraction (fu, fu = Cu / Ct) for the TDM of ATV. The variability of Cu and the corresponding fu of ATV were explored in 43 patients treated with ATV for an average of 13...
September 24, 2016: Fundamental & Clinical Pharmacology
https://www.readbyqxmd.com/read/27642072/genetic-engineering-of-cho-cells-for-viral-resistance-to-minute-virus-of-mice
#9
Joaquina X Mascarenhas, Nikolay Korokhov, Lisa Burger, Ademola Kassim, Jason Tuter, Daniel Miller, Trissa Borgschulte, Henry J George, Audrey Chang, David J Pintel, David Onions, Kevin J Kayser
Contamination by the parvovirus minute virus of mice (MVM) remains a challenge in Chinese hamster ovary (CHO) biopharmaceutical production processes. Although infrequent, infection of a bioreactor can be catastrophic for a manufacturer, can impact patient drug supply and safety, and can have regulatory implications. We evaluated engineering a CHO parental cell line (CHOZN(®) GS(-/-) ) to create a new host cell line that is resistant to MVM infection by modifying the major receptors used by the virus to enter cells...
September 19, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27590021/international-validation-of-a-urinary-biomarker-panel-for-identification-of-active-lupus-nephritis-in-children
#10
Eve Mary Dorothy Smith, Andrea Lyn Jorgensen, Angela Midgley, Louise Oni, Beatrice Goilav, Chaim Putterman, Dawn Wahezi, Tamar Rubinstein, Diana Ekdawy, Rachel Corkhill, Caroline Ann Jones, Stephen David Marks, Paul Newland, Clarissa Pilkington, Kjell Tullus, Michael William Beresford
BACKGROUND: Conventional markers of juvenile-onset systemic lupus erythematosus (JSLE) disease activity fail to adequately identify lupus nephritis (LN). While individual novel urine biomarkers are good at detecting LN flares, biomarker panels may improve diagnostic accuracy. The aim of this study was to assess the performance of a biomarker panel to identify active LN in two international JSLE cohorts. METHODS: Novel urinary biomarkers, namely vascular cell adhesion molecule-1 (VCAM-1), monocyte chemoattractant protein 1 (MCP-1), lipocalin-like prostaglandin D synthase (LPGDS), transferrin (TF), ceruloplasmin, alpha-1-acid glycoprotein (AGP) and neutrophil gelatinase-associated lipocalin (NGAL), were quantified in a cross-sectional study that included participants of the UK JSLE Cohort Study (Cohort 1) and validated within the Einstein Lupus Cohort (Cohort 2)...
September 3, 2016: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/27583708/inflammation-and-biomarkers-of-micronutrient-status
#11
David I Thurnham, Christine A Northrop-Clewes
PURPOSE OF REVIEW: Investigations to discover new biomarkers of nutrition highlighted the fact that inflammation and infection were cross-cutting issues complicating interpretation of status. Collaborative groups of nutritionists, immunologists, clinicians and statisticians were set up to investigate the issues, and some are now reporting their findings. RECENT FINDINGS: Recent work on the vitamins A, D, E and C and the elements iron, zinc and selenium are reported in this review...
November 2016: Current Opinion in Clinical Nutrition and Metabolic Care
https://www.readbyqxmd.com/read/27565792/analysis-of-fucosylation-in-liver-secreted-n-glycoproteins-from-human-hepatocellular-carcinoma-plasma-using-liquid-chromatography-with-tandem-mass-spectrometry
#12
Eun Sun Ji, Heeyoun Hwang, Gun Wook Park, Ju Yeon Lee, Hyun Kyoung Lee, Na Young Choi, Hoi Keun Jeong, Kwang Hoe Kim, Jin Young Kim, Seungho Lee, Yeong Hee Ahn, Jong Shin Yoo
Fucosylation of N-glycoproteins has been implicated in various diseases, such as hepatocellular carcinoma (HCC). However, few studies have performed site-specific analysis of fucosylation in liver-secreted proteins. In this study, we characterized the fucosylation patterns of liver-secreted proteins in HCC plasma using a workflow to identify site-specific N-glycoproteins, where characteristic B- and/or Y-ion series with and without fucose in collision-induced dissociation were used in tandem mass spectrometry...
November 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27527627/predictors-of-inflammation-in-a-cohort-of-bolivian-infants-and-toddlers
#13
Rachel M Burke, Parminder S Suchdev, Paulina A Rebolledo, Anna M Fabiszewski de Aceituno, Rita Revollo, Volga Iñiguez, Mitchel Klein, Carolyn Drews-Botsch, Juan S Leon
Inflammation has been associated with cardiovascular disease and other health outcomes in children and adults, yet few longitudinal data are available on prevalence and predictors of inflammation in infants. We aimed to identify the prevalence of inflammation in a cohort of Bolivian infants and estimate its association with acute (recent illnesses) and chronic (overweight, stunting) morbidities and potential pathogen exposure (represented by water, sanitation, and hygiene [WASH] resources). We measured plasma concentrations of two acute phase proteins (C-reactive protein [CRP], marking acute inflammation, and alpha(1)-acid-glycoprotein [AGP], marking chronic inflammation) at three time points (target 2, 6-8, and 12-18 months)...
October 5, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27485861/association-between-nutritional-status-inflammatory-condition-and-prognostic-indexes-with-postoperative-complications-and-clinical-outcome-of-patients-with-gastrointestinal-neoplasia
#14
Milena Damasceno de Souza Costa, Camila Yandara Sousa Vieira de Melo, Ana Carolina Ribeiro de Amorim, Dilênia de Oliveira Cipriano Torres, Ana Célia Oliveira Dos Santos
The aim of this study is to describe and relate nutritional and inflammatory status and prognostic indexes with postoperative complications and clinical outcome of patients with gastrointestinal malignancies. Twenty-nine patients were evaluated; nutritional assessment was carried out by subjective and objective parameters; albumin, pre-albumin, C-reactive protein (CRP), and alpha-1-acid glycoprotein (AGP) were determined. To assess prognosis, the Glasgow scale, the Prognostic Inflammatory Nutritional Index (PINI), and CRP/albumin ratio were used; the clinical outcomes considered were hospital discharge and death...
October 2016: Nutrition and Cancer
https://www.readbyqxmd.com/read/27461250/prospective-analysis-in-gist-patients-on-the-role-of-alpha-1-acid-glycoprotein-in-imatinib-exposure
#15
Sander Bins, Karel Eechoute, Jacqueline S L Kloth, Femke M de Man, Astrid W Oosten, Peter de Bruijn, Stefan Sleijfer, Ron H J Mathijssen
BACKGROUND: For imatinib, a relationship between systemic exposure and clinical outcome has been suggested. Importantly, imatinib concentrations are not stable and decrease over time, for which several mechanisms have been suggested. In this study, we investigated if a decrease in alpha-1 acid glycoprotein (AGP) is the main cause of the lowering in imatinib exposure over time. METHODS: We prospectively measured imatinib trough concentration (C min) values in 28 patients with gastrointestinal stromal tumours, at 1, 3 and 12 months after the start of imatinib treatment...
July 26, 2016: Clinical Pharmacokinetics
https://www.readbyqxmd.com/read/27427194/the-effects-of-orally-administered-bacillus-coagulans-and-inulin-on-prevention-and-progression-of-rheumatoid-arthritis-in-rats
#16
Khadijeh Abhari, Seyed Shahram Shekarforoush, Saeid Hosseinzadeh, Saeid Nazifi, Javad Sajedianfard, Mohammad Hadi Eskandari
BACKGROUND: Probiotics have been considered as an approach to addressing the consequences of different inflammatory disorders. The spore-forming probiotic strain Bacillus coagulans has demonstrated anti-inflammatory and immune-modulating effects in both animals and humans. The prebiotic inulin also potentially affects the immune system as a result of the change in the composition or fermentation profile of the gastrointestinal microbiota. OBJECTIVE: In the present study, an in vivo model was conducted to investigate the possible influences of probiotic B...
2016: Food & Nutrition Research
https://www.readbyqxmd.com/read/27354006/multifucosylated-alpha-1-acid-glycoprotein-as-a-novel-marker-for-hepatocellular-carcinoma
#17
Kazuhiro Tanabe, Kae Kitagawa, Nozomi Kojima, Sadayo Iijima
High-sensitivity and -specificity diagnostic techniques to detect early-stage hepatocellular carcinoma (HCC) are in high demand. Screening with serum HCC markers, such as alpha-fetoprotein, is not practical because they possess poor sensitivity and specificity. As such, we focused on glycan alterations of glycoproteins found in patient sera in an attempt to discover novel HCC markers that are more specific and sensitive than current HCC markers. Sera from 42 HCC patients and 80 controls, composed of 27 chronic hepatitis B patients, 26 chronic hepatitis C patients, and 27 healthy volunteers, were analyzed in this study...
September 2, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27336623/platelets-proteomic-profiles-of-acute-ischemic-stroke-patients
#18
Ozge Cevik, Ahmet Tarik Baykal, Azize Sener
Platelets play a crucial role in the pathogenesis of stroke and antiplatelet agents exist for its treatment and prevention. Through the use of LC-MS based protein expression profiling, platelets from stroke patients were analyzed and then correlated with the proteomic analyses results in the context of this disease. This study was based on patients who post ischemic stroke were admitted to hospital and had venous blood drawn within 24 hrs of the incidence. Label-free protein expression analyses of the platelets' tryptic digest was performed in triplicate on a UPLC-ESI-qTOF-MS/MS system and ProteinLynx Global Server (v2...
2016: PloS One
https://www.readbyqxmd.com/read/27304613/paraneoplastic-epilepsy
#19
REVIEW
Anna Serafini, Rimas V Lukas, Stephen VanHaerents, Peter Warnke, James X Tao, Sandra Rose, Shasha Wu
Epilepsy can be a manifestation of paraneoplastic syndromes which are the consequence of an immune reaction to neuronal elements driven by an underlying malignancy affecting other organs and tissues. The antibodies commonly found in paraneoplastic encephalitis can be divided into two main groups depending on the target antigen: 1) antibodies against neuronal cell surface antigens, such as against neurotransmitter (N-methyl-d-aspartate (NMDA), alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), gamma-aminobutyric acid (GABA)) receptors, ion channels (voltage-gated potassium channel (VGKC)), and channel-complex proteins (leucine rich, glioma inactivated-1 glycoprotein (LGI1) and contactin-associated protein-2 (CASPR2)) and 2) antibodies against intracellular neuronal antigens (Hu/antineuronal nuclear antibody-1 (ANNA-1), Ma2/Ta, glutamate decarboxylase 65 (GAD65), less frequently to CV2/collapsin response mediator protein 5 (CRMP5))...
August 2016: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/27246700/identification-of-novel-n-glycosylation-sites-at-noncanonical-protein-consensus-motifs
#20
Mark S Lowenthal, Kiersta S Davis, Trina Formolo, Lisa E Kilpatrick, Karen W Phinney
N-glycosylation of proteins is well known to occur at asparagine residues that fall within the canonical consensus sequence N-X-S/T but has also been identified at a small number of asparagine residues within N-X-C motifs, including the N491 residue of human serotransferrin. Here we report novel glycosylation sites within noncanonical consensus motifs, in the conformation N-X-C, based on mass spectrometry analysis of partially deglycosylated glycopeptide targets. Alpha-1-acid glycoprotein (A1AG) and serotransferrin (Tf) were observed for the first time to be N-glycosylated on asparagine residues within a total of six unique noncanonical motifs...
July 1, 2016: Journal of Proteome Research
keyword
keyword
34162
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"