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Alpha-1-acid glycoprotein

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https://www.readbyqxmd.com/read/29234088/tear-proteome-analysis-in-ocular-surface-diseases-using-label-free-lc-ms-ms-and-multiplexed-microarray-biomarker-validation
#1
Javier Soria, Arantxa Acera, Jesús Merayo-LLoves, Juan A Durán, Nerea González, Sandra Rodriguez, Nikitas Bistolas, Soeren Schumacher, Frank F Bier, Harald Peter, Walter Stöcklein, Tatiana Suárez
We analyzed the tear film proteome of patients with dry eye (DE), meibomian gland dysfunction (MGD), and normal volunteers (CT). Tear samples were collected from 70 individuals. Of these, 37 samples were analyzed using spectral-counting-based LC-MS/MS label-free quantitation, and 33 samples were evaluated in the validation of candidate biomarkers employing customized antibody microarray assays. Comparative analysis of tear protein profiles revealed differences in the expression levels of 26 proteins, including protein S100A6, annexin A1, cystatin-S, thioredoxin, phospholipase A2, antileukoproteinase, and lactoperoxidase...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29231704/site-specific-glycan-heterogeneity-characterization-by-hilic-solid-phase-extraction-rplc-fractionation-and-capillary-zone-electrophoresis-electrospray-ionization-tandem-mass-spectrometry
#2
Yanyan Qu, Liangliang Sun, Zhenbin Zhang, Norman J Dovichi
Reversed-phase chromatographic separation of glycopeptides tends to be dominated by the peptide composition. In contrast, capillary zone electrophoresis separation of glycopeptides is particularly sensitive to the sialic acid composition of the glycan. In this paper, we combine the two techniques to achieve superior N-glycopeptide analysis. Glycopeptides were first isolated from a tryptic digest using HILIC solid-phase extraction. The glycopeptides were separated using reversed-phase UHPLC to generate four fractions corresponding to different peptide backbones...
December 12, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29230915/periapical-infection-may-affect-birth-outcomes-via-systemic-inflammation
#3
U Harjunmaa, R Doyle, J Järnstedt, S Kamiza, J M Jorgensen, C P Stewart, L Shaw, L Hallamaa, U Ashorn, N Klein, K G Dewey, K Maleta, P Ashorn
OBJECTIVES: Maternal dental periapical infections are associated with preterm birth and intrauterine growth restriction. This study investigates if the association is mediated through bacterial spread from periapical lesions to placenta (direct pathway) or systemic inflammatory reaction (indirect pathway). MATERIALS AND METHODS: We compared birth outcomes in Malawian mothers with and without periapical infection. As markers of a direct pathway, we identified placental bacteria using a 16S rDNA approach, and assessed histological evidence of inflammation in the placenta and amniotic membranes...
December 12, 2017: Oral Diseases
https://www.readbyqxmd.com/read/29207246/multiple-reaction-monitoring-for-the-quantitation-of-serum-protein-glycosylation-profiles-application-to-ovarian-cancer
#4
Suzanne Miyamoto, Carol D Stroble, Sandra Taylor, Qiuting Hong, Carlito B Lebrilla, Gary S Leiserowitz, Kyoungmi Kim, L Renee Ruhaak
Protein glycosylation fingerprints are widely recognized as potential markers for disease states and indeed differential glycosylation has been identified in multiple types of autoimmune diseases and several types of cancer. However, releasing the glycans leave the glycoproteins unknown, therefore there exists a need for high-throughput methods that allow quantification of site- and protein- specific glycosylation patterns from complex biological mixtures. In this study, a targeted multiple reaction monitoring (MRM)-based method for the protein- and site- specific quantitation involving serum proteins immunoglobulins A, G and M, alpha-1-antitrypsin, transferrin, alpha-2-macroglobulin, haptoglobin, alpha-1-acid glycoprotein and complement C3 was developed...
December 5, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29182756/acute-phase-proteins-and-stress-markers-in-the-immediate-response-to-a-combined-vaccination-against-newcastle-disease-and-infectious-bronchitis-viruses-in-specific-pathogen-free-spf-layer-chicks
#5
Haider Kaab, Maureen M Bain, Peter David Eckersall
Vaccination is an important tool in poultry health, but is itself a stressor often resulting in a reduction in feed intake, body weight gain, and nutrient digestibility. In other species, vaccination is associated with an immediate acute-phase response. As an important immune parameter, the circulating heterophil/lymphocyte (H/L) ratio is a well-recognized parameter of stress in poultry. In this study, the effects of a routinely used commercial poultry vaccine on the acute phase response (APR) and H/L ratios in specific pathogen-free (SPF) layer chicks was examined to determine if post vaccination (PV) stress and an APR occur...
November 22, 2017: Poultry Science
https://www.readbyqxmd.com/read/29158276/seventy-five-years-of-research-on-protein-binding-do-we-ever-understand
#6
Axel Dalhoff
This review summarizes evidence that the impact of protein binding of the activity of antibiotics is multifacetted and more complex than indicated by the numerical value of protein binding alone. A plethora of studies has proven that protein binding of antibiotics matters - the free fraction only is antibacterially active and governs pharamcokinetics. Several studies have indicated that independent from protein binding immunoglobulin G, albumin, α-1-acid-glycoprotein, and pulmonary surfactant acted synergistically with antibacterial agents, thus suggesting that some intrinsic properties of serum proteins may have mediated serum antibiotic synergisms...
November 20, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29154737/significant-changes-in-itih4-ahsg-orm1-and-cd46-content-in-milk-fat-globule-membrane-proteins-of-ketotic-dairy-cows
#7
Sansi Gao, Wei Yang, Hongjiang Yu, Runqi Liu, Zhihao Dong, Hongyou Zhang, Cheng Xia, Chuang Xu
High concentrations of non-esterified fatty acid (NEFA) and β-hydroxybutyrate (BHBA) in cows' blood caused by ketosis are associated with inflammatory states. We hypothesised that ketosis in postparturient dairy cows would result in altered levels on inflammation-related proteins not only in plasma but also in the milk fat globule membranes (MFGM). Thirty cows were selected from a dairy farm in Heilongjiang, China. Inflammatory milk fat globule membrane proteins were detected using ELISA kits, and a fully automatic biochemical analyser was used to measure the concentrations of BHBA, NEFA, glucose (GLU) and triglyceride (TG) in plasma...
November 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/29138201/effect-of-iron-and-zinc-biofortified-pearl-millet-consumption-on-growth-and-immune-competence-in-children-aged-12-18-months-in-india-study-protocol-for-a-randomised-controlled-trial
#8
Saurabh Mehta, Julia L Finkelstein, Sudha Venkatramanan, Samantha L Huey, Shobha A Udipi, Padmini Ghugre, Caleb Ruth, Richard L Canfield, Anura V Kurpad, Ramesh D Potdar, Jere D Haas
INTRODUCTION: Biofortified crops represent a sustainable agricultural solution for the widespread micronutrient malnutrition in India and other resource-limited settings. This study aims to investigate the effect of the consumption of foods prepared with iron- and zinc-biofortified pearl millet (FeZn-PM) by children on biomarkers of iron and zinc status, growth, and immune function. METHODS AND ANALYSIS: We will conduct a randomised controlled feeding trial in identified slums of Mumbai, India among 200 children aged between 12 and 18 months...
November 14, 2017: BMJ Open
https://www.readbyqxmd.com/read/29076384/pharmacology-and-pharmacokinetics-of-imatinib-in-pediatric-patients
#9
Meinolf Suttorp, Martin Bornhäuser, Markus Metzler, Frédéric Millot, Eberhard Schleyer
The tyrosine kinase inhibitor (TKI) imatinib was rationally designed to target BCR-ABL1 which is constitutively activated in chronic myeloid leukemia (CML). Following the tremendous success in adults, imatinib also became licensed for treatment of CML in minors. The rarity of pediatric CML hampers the conduction of formal trials. Thus, imatinib is still the single TKI approved for CML treatment in childhood. Areas covered: This review attempts to provide an overview of the literature on pharmacology, pharmacokinetic, and pharmacogenetic of imatinib concerning pediatric CML treatment...
October 27, 2017: Expert Review of Clinical Pharmacology
https://www.readbyqxmd.com/read/29075332/hypermethylation-in-the-promoter-of-the-mthfr-gene-is-associated-with-diabetic-complications-and-biochemical-indicators
#10
Mayara Karla Dos Santos Nunes, Alexandre Sérgio Silva, Isabella Wanderley de Queiroga Evangelista, João Modesto Filho, Cecília Neta Alves Pegado Gomes, Rayner Anderson Ferreira do Nascimento, Rafaella Cristhine Pordeus Luna, Maria José de Carvalho Costa, Naila Francis Paulo de Oliveira, Darlene Camati Persuhn
BACKGROUND: DNA methylation is an epigenetic mechanism for regulating the transcription of many genes and has been linked to the development of various diseases. A promising gene to investigate is methylenetetrahydrofolate reductase (MTHFR), since the enzyme methylenetetrahydrofolate reductase (MTHFR) promotes methyl radical synthesis in the homocysteine cycle and can provide methyl groups for DNA methylation. In addition, several studies have correlated gene polymorphisms of this enzyme with a greater risk of diabetes, but little is known regarding the relationship between epigenetic changes in this gene and diabetes and its complications...
2017: Diabetology & Metabolic Syndrome
https://www.readbyqxmd.com/read/29070467/-comparison-of-postoperative-drainage-and-systemic-trauma-response-after-endoscopic-and-traditional-near-total-thyroidectomy
#11
Yong-Quan Zhang, Zhi-Chao Li, Fei Chen, Hong-Juan Wang, Qiang Li
OBJECTIVE: To investigate the difference of postoperative drainage and systemic trauma response between endoscopic and traditional near total thyroidectomy to provide the basis for selecting appropriate operative methods. METHODS: In this prospective clinical controlled study, 80 patientsscheduled for near total bilateral thyroidectomy for the first time were divided equally into endoscopic surgery group (group A) and open surgery group (group B). The total drainage volume after operation, postoperative extubation time, and postoperative daily drainage volume were recorded after the operation...
October 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/29042303/changes-in-serum-proteins-in-dogs-with-ehrlichia-canis-infection
#12
Damián Escribano, Hüseyin Cihan, Silvia Martínez-Subiela, Pinar Levent, Meric Kocaturk, Nilüfer Aytug, Jose Joaquin Cerón, Asta Tvarijonaviciute, Zeki Yilmaz
The aim of this study was the identification of proteins differentially represented in the serum proteome of seropositive dogs with (Group 1) and without (Group 2) clinical-pathologic signs consistent with ehrlichiosis compared to healthy control dogs. Serum samples were collected from 20 dogs of various breeds with naturally occurring ehrlichiosis (10 dogs belonged to Group 1 and 10 to Group 2) and 10 healthy dogs. Two-dimensional electrophoresis (2DE) of pooled serum for each of the group of dogs were run in triplicate...
October 16, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29031784/isobaric-tags-for-relative-and-absolute-quantitation-itraq-based-proteomics-for-the-investigation-of-the-effect-of-huganqingzhi-on-non-alcoholic-fatty-liver-disease-in-rats
#13
Xiaorui Yao, Fan Xia, Waijiao Tang, Chunxin Xiao, Miaoting Yang, Benjie Zhou
ETHNOPHARMACOLOGICAL RELEVANCE: Hugan Qingzhi tablet (HQT), a traditional Chinese medicine formula has been adopted for preventing and treating nonalcoholic fatty liver disease (NAFLD). AIM: In order to explore the anti-NAFLD mechanisms of HQT, iTRAQ-based proteomic was employed to investigate the expression profiles of proteins in NAFLD rats induced by high-fat diet after HQT treatment. MATERIALS AND METHODS: The NAFLD rat model was administrated with high-fat diet (HFD) for 12weeks...
October 13, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29031301/zwitterionic-hydrophilic-interaction-capillary-liquid-chromatography-coupled-to-tandem-mass-spectrometry-for-the-characterization-of-human-alpha-acid-glycoprotein-n-glycan-isomers
#14
Montserrat Mancera-Arteu, Estela Giménez, José Barbosa, Rosa Peracaula, Victòria Sanz-Nebot
In this work, a μZIC-HILIC-MS/MS methodology was established in negative ion mode for the characterization of glycan isomers. The possibility to separate the glycan isomers by the μZIC-HILIC strategy coupled to a high resolution tandem mass spectrometry detection permitted us to obtain valuable information about each glycan structure. The most important diagnostic ion fragments previously described to characterize structural features of glycans, were evaluated in this study using hAGP as model glycoprotein...
October 23, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28992104/a-simplified-and-sensitive-method-to-identify-alzheimer-s-disease-biomarker-candidates-using-patient-derived-induced-pluripotent-stem-cells-ipscs
#15
Keiro Shirotani, Kazuya Matsuo, Sumio Ohtsuki, Takeshi Masuda, Masashi Asai, Yumiko Kutoku, Yutaka Ohsawa, Yoshihide Sunada, Takayuki Kondo, Haruhisa Inoue, Nobuhisa Iwata
We developed a simplified and sensitive method to identify Alzheimer's disease (AD) biomarker candidates by a quantitative and targeted proteomic analysis (combination of liquid chromatography tandem mass spectrometry and multiplexed-multiple reaction monitoring/selected reaction monitoring analysis) of culture media from neurons differentiated from induced pluripotent stem cells (iPSCs) established from AD patients. We found that alpha-1-acid glycoprotein (ORM1) was decreased in the culture media of AD-iPSC-derived neurons, consistent with previous observations for AD patient cerebrospinal fluid, thus validating our new strategy...
September 4, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28948673/piperine-decreases-binding-of-drugs-to-human-plasma-and-increases-uptake-by-brain-microvascular-endothelial-cells
#16
Raghvendra K Dubey, Brigitte Leeners, Bruno Imthurn, Gabriele Susanne Merki-Feld, Marinella Rosselli
We previously reported that piperine, an active alkaloidal principal of black and long peppers, enhances drug bioavailability by inhibiting drug metabolism. Another mechanism influencing drug availability/uptake is its free fraction. Since piperine is highly lipophilic, we hypothesize that it could also interact with drugs through binding displacement and influence their bioavailability. Accordingly, using equilibrium dialysis, we investigated whether piperine alters the binding of model drug ligands, that is flunitrazepam, diazepam, warfarin, salicylic acid, propranolol, lidocaine, and disopyramide to human plasma (n = 4)...
September 26, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28944674/analysis-of-o-glycopeptides-by-acetone-enrichment-and-capillary-electrophoresis-mass-spectrometry
#17
Montserrat Mancera-Arteu, Estela Giménez, Fernando Benavente, José Barbosa, Victòria Sanz-Nebot
Acetone precipitation was evaluated as a rapid, simple, low-cost, and efficient method for the selective purification of O-glycopeptides from enzymatic digests of glycoproteins. Ovalbumin (OVA), human and bovine α1-acid glycoprotein (hAGP and bAGP), human apolipoprotein C-III (APO-C3), and recombinant human erythropoietin (rhEPO) were used to obtain enzymatic digests with a broad and varied set of peptides, N-glycopeptides, and O-glycopeptides. After digestion and before capillary electrophoresis mass spectrometry (CE-MS) analysis, the amount of ice-cold acetone added to the digests was optimized to maximize recoveries of O-glycopeptides...
October 10, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28944578/impact-of-a-central-scaffold-on-the-binding-affinity-of-fragment-pairs-isolated-from-dna-encoded-self-assembling-chemical-libraries
#18
Martina Bigatti, Alberto Dal Corso, Sara Vanetti, Samuele Cazzamalli, Ulrike Rieder, Jörg Scheuermann, Dario Neri, Filippo Sladojevich
The screening of encoded self-assembling chemical libraries allows the identification of fragment pairs that bind to adjacent pockets on target proteins of interest. For practical applications, it is necessary to link these ligand pairs into discrete organic molecules, devoid of any nucleic acid component. Here we describe the discovery of a synergistic binding pair for acid alpha-1 glycoprotein and a chemical strategy for the identification of optimal linkers, connecting the two fragments. The procedure yielded a set of small organic ligands, the best of which exhibited a dissociation constant of 9...
September 24, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28903783/the-purification-of-reduced-%C3%AE-2-glycoprotein-i-showed-its-native-activity-in-vitro
#19
Saijun Zhou, Ming Lu, Jiantong Zhao, Shuaihui Liu, Xin Li, Rui Zhang, Hongyan Liu, Pei Yu
BACKGROUND: New evidence has shown that reduced β2-glycoprotein I (β2GPI) has anti-oxidative stress and anti-inflammatory activity. However, the details are still poorly understood. This study aims to prepare stable reduced β2GPI with its native bioactivity in vitro. METHODS: Human β2GPI was purified from plasma first with perchloric acid precipitation and then purified with a series of chromatography methods including Sephadex G-25 desalting, SP HP, AF-heparin HC-650 M, and Sephacryl S-200...
September 13, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28875469/a-panel-of-autoantibodies-against-neural-proteins-as-peripheral-biomarker-for-pesticide-induced-neurotoxicity
#20
Heba Allah Abd El Rahman, Mohamed Salama, Seham A Gad El-Hak, Mona A El-Harouny, Passent ElKafrawy, Mohamed B Abou-Donia
In the present study, we screened the sera of subjects chronically exposed to mixtures of pesticides (composed mainly of organophosphorus compounds (OPs) and others) and developed neurological symptoms for the presence of autoantibodies against cytoskeletal neural proteins. OPs have a well-characterized clinical profile resulting from acute cholinergic crisis. However, some of these compounds cause neuronal degeneration and demyelination known as organophosphorus compound-induced delayed neurotoxicity (OPIDN) and/or organophosphorus compound-induced chronic neurotoxicity (OPICN)...
September 5, 2017: Neurotoxicity Research
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