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https://www.readbyqxmd.com/read/27915161/intestinal-multidrug-resistance-associated-protein-2-is-down-regulated-in-fructose-fed-rats
#1
Ana Sofía Londero, Maite Rocío Arana, Virginia Gabriela Perdomo, Guillermo Nicolás Tocchetti, Felipe Zecchinati, Carolina Inés Ghanem, María Laura Ruiz, Juan Pablo Rigalli, Aldo Domingo Mottino, Fabiana García, Silvina Stella Maris Villanueva
Expression and activity of jejunal multidrug resistance-associated protein 2 (Mrp2) and glutathione-S-transferase (GST) were examined in fructose fed Wistar rats, an experimental model of metabolic syndrome. Animals were fed on (a) control diet or (b) control diet plus 10% w/vol fructose in the drinking water. Mrp2 and the α class of GST proteins as well as their corresponding mRNAs were decreased, suggesting a transcriptional regulation by fructose. Confocal microscopy studies reaffirmed down-regulation of Mrp2...
November 9, 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/27895892/sexual-dimorphism-in-the-mast-cell-transcriptome-and-the-pathophysiological-responses-to-immunological-and-psychological-stress
#2
Emily Mackey, Saravanan Ayyadurai, Calvin S Pohl, Susan D' Costa, Yihang Li, Adam J Moeser
BACKGROUND: Biological sex plays a prominent role in the prevalence and severity of a number of important stress-related gastrointestinal and immune-related diseases including IBS and allergy/anaphylaxis. Despite the establishment of sex differences in these diseases, the underlying mechanisms contributing to sex differences remain poorly understood. The objective of this study was to define the role of biological sex on mast cells (MCs), an innate immune cell central to the pathophysiology of many GI and allergic disorders...
2016: Biology of Sex Differences
https://www.readbyqxmd.com/read/27892752/concentration-dependence-of-nitroxyl-spin-probes-in-liposomal-solution-electron-spin-resonance-and-overhauser-enhanced-magnetic-resonance-studies
#3
Meenakumari V, Hideo Utsumi, A Jawahar, A Milton Franklin Benial
In this work, the detailed studies of electron spin resonance (ESR) and Overhauser enhanced magnetic resonance imaging (OMRI) were carried out for permeable nitroxyl spin probe, MC-PROXYL as a function of agent concentration in liposomal solution. In order to compare the impermeable nature of nitroxyl radical, the study was also carried out only at 2mM concentration of carboxy-PROXYL. The ESR parameters were estimated using L- band and 300 MHz ESR spectrometers. The line width broadening was measured as a function of agent concentration in liposomal solution...
November 28, 2016: Journal of Liposome Research
https://www.readbyqxmd.com/read/27890918/2-hydroxy-3-methoxybenzoic-acid-attenuates-mast-cell-mediated-allergic-reaction-in-mice-via-modulation-of-the-fc%C3%AE%C2%B5ri-signaling-pathway
#4
Yeon-Yong Kim, In-Gyu Je, Min Jong Kim, Byeong-Cheol Kang, Young-Ae Choi, Moon-Chang Baek, Byungheon Lee, Jin Kyeong Choi, Hae Ran Park, Tae-Yong Shin, Soyoung Lee, Seung-Bin Yoon, Sang-Rae Lee, Dongwoo Khang, Sang-Hyun Kim
Mast cells are important effector cells in immunoglobulin (Ig) E-mediated allergic reactions such as asthma, atopic dermatitis and rhinitis. Vanillic acid, a natural product, has shown anti-oxidant and anti-inflammatory activities. In the present study, we investigated the anti-allergic inflammatory effects of ortho-vanillic acid (2-hydroxy-3-methoxybenzoic acid, o-VA) that was a derivative of vanillic acid isolated from Amomum xanthioides. In mouse anaphylaxis models, oral administration of o-VA (2, 10, 50 mg/kg) dose-dependently attenuated ovalbumin-induced active systemic anaphylaxis and IgE-mediated cutaneous allergic reactions such as hypothermia, histamine release, IgE production and vasodilation; administration of o-VA also suppressed the mast cell degranulator compound 48/80-induced anaphylaxis...
November 28, 2016: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/27889650/large-dose-hyperpolarized-water-with-dissolution-dnp-at-high-magnetic-field
#5
Kasper Wigh Lipsø, Sean Bowen, Oleksandr Rybalko, Jan Henrik Ardenkjær-Larsen
We demonstrate a method for the preparation of hyperpolarized water by dissolution Dynamic Nuclear Polarization at high magnetic field. Protons were polarized at 6.7T and 1.1K to >70% with frequency modulated microwave irradiation at 188GHz. 97.2±0.7% of the radical was extracted from the sample in the dissolution in a two-phase system. 16±1mL of 5.0M (1)H in D2O with a polarization of 13.0±0.9% in the liquid state was obtained, corresponding to an enhancement factor of 4000±300 compared to the thermal equilibrium at 9...
November 18, 2016: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/27881053/oxytocin-provides-protection-against-diabetic-polyneuropathy-in-rats
#6
Oytun Erbas, Dilek Taşkıran, Fatih Oltulu, Altuğ Yavaşoğlu, Saylav Bora, Okan Bilge, Bilge Piri Çınar, Gönül Peker
PURPOSE: The aim of the present study is to investigate the protective effects of oxytocin (OT) on diabetic neuropathy (DNP) in rats. MATERIALS AND METHODS: Eighteen rats were used to induce diabetes using single dose streptozotocin (STZ, 60 mg/kg). Diabetic DNP was verified by electromyography (EMG) and motor function test on 21st day following STZ injection. Six rats served as naïve control group and received no drug (n = 6). Following EMG, diabetic rats were randomly divided into three groups and administered with either 1 ml/kg saline or 80 μg/kg OT or 160 μg/kg OT intraperitoneally for four weeks...
November 24, 2016: Neurological Research
https://www.readbyqxmd.com/read/27879035/dynamic-nuclear-polarization-provides-new-insights-into-chromophore-structure-in-phytochrome-photoreceptors
#7
Daniel Stöppler, Chen Song, Barth-Jan van Rossum, Michel-Andreas Geiger, Christina Lang, Maria-Andrea Mroginski, Anil Pandurang Jagtap, Snorri Th Sigurdsson, Jörg Matysik, Jon Hughes, Hartmut Oschkinat
Phytochromes are red/far-red photochromic photoreceptors acting as master regulators of development in higher plants, thereby controlling transcription of about 20 % of their genes. Light-induced isomerization of the bilin chromophore leads to large rearrangements in protein structure, whereby the role of protonation dynamics and charge distribution is of particular interest. To help unravel the inherent mechanisms, we present two-dimensional dynamic nuclear polarization (DNP) enhanced solid-state magic-angle spinning (MAS) NMR spectra of the functional sensory module of the cyanobacterial phytochrome Cph1...
November 23, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27859555/pyruvate-cellular-uptake-and-enzymatic-conversion-probed-by-dissolution-dnp-nmr-the-impact-of-overexpressed-membrane-transporters
#8
Riccardo Balzan, Laetitia Fernandes, Laetitia Pidial, Arnaud Comment, Bertrand Tavitian, Paul R Vasos
Pyruvate membrane crossing and its LDH-mediated conversion to lactate in cells featuring different levels of expression of membrane monocarboxylate transporters (MCT4) was probed using dissolution DNP-enhanced NMR. Hyperpolarized (13) C-1 labeled pyruvate was transferred to suspensions of rodent tumor cells CCL39. The pyruvate-to-lactate conversion rate monitored by dissolution-DNP NMR in CCL39 featuring native MCT4 expression level was lower than the rate observed for cells in which the human MCT4 gene was over-expressed...
November 12, 2016: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/27859255/modulation-of-multidrug-resistance-associated-proteins-function-in-erythrocytes-in-glycerol-induced-acute-renal-failure-rats
#9
Aoi Matsushima, Keisuke Oda, Nobuhiro Mori, Teruo Murakami
OBJECTIVES: Evaluation of the function of multidrug resistance-associated proteins (MRPs) expressed in erythrocytes and screening of endogenous MRPs modulator(s) in glycerol-induced acute renal failure (ARF) rats. METHODS: Concentrations of 2,4-dinitrophenyl-S-glutathione (DNP-SG), a substrate for MRPs, in erythrocytes after administration of 1-chloro-2,4-dintrobenzene (CDNB), a precursor of DNP-SG, were determined in control and ARF rats. The screening of endogenous MRPs modulator(s) was performed using washed erythrocytes and inside-out erythrocyte membrane vesicles (IOVs) in vitro...
November 18, 2016: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/27800632/the-dnp-mph-dual-degree-an-innovative-graduate-education-program-for-advanced-public-health-nursing
#10
Kathy Shaw, Scott Harpin, Geraldine Steinke, Marilyn Stember, Marilyn Krajicek
Strong professional priorities, evolving Affordable Care Act requirements, and a significantly limited public health nursing workforce prompted the University of Colorado College of Nursing to collaborate with the School of Public Health to implement one of the first Doctor of Nursing Practice/Master of Public Health dual degree programs in the nation. Federal grant funding supported the development, implementation, and evaluation of this unique post-baccalaureate dual degree program, for which there were no roadmaps, models, or best practices to follow...
October 31, 2016: Public Health Nursing
https://www.readbyqxmd.com/read/27798283/mitochondrial-atp-is-required-for-the-maintenance-of-membrane-integrity-in-stallion-spermatozoa-whereas-motility-requires-both-glycolysis-and-oxidative-phosphorylation
#11
M Plaza Davila, P Martin Muñoz, J M Gallardo Bolaños, T A E Stout, B M Gadella, J A Tapia, C Balao da Silva, C Ortega Ferrusola, F J Peña
To investigate the hypothesis that oxidative phosphorylation is a major source of ATP to fuel stallion sperm motility, oxidative phosphorylation was suppressed using the mitochondrial uncouplers CCCP and 2,4,-dinitrophenol (DNP) and by inhibiting mitochondrial respiration at complex IV using sodium cyanide or at the level of ATP synthase using oligomycin-A. As mitochondrial dysfunction may also lead to oxidative stress, production of reactive oxygen species was monitored simultaneously. All inhibitors reduced ATP content, but oligomycin-A did so most profoundly...
December 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27791210/temperature-dependence-of-cross-effect-dynamic-nuclear-polarization-in-rotating-solids-advantages-of-elevated-temperatures
#12
Michel-Andreas Geiger, Marcella Orwick-Rydmark, Katharina Märker, W Trent Franks, Dmitry Akhmetzyanov, Daniel Stöppler, Maximilian Zinke, Edgar Specker, Marc Nazaré, Anne Diehl, Barth-Jan van Rossum, Fabien Aussenac, Thomas Prisner, Ümit Akbey, Hartmut Oschkinat
Dynamic nuclear polarization exploits electron spin polarization to boost signal-to-noise in magic-angle-spinning (MAS) NMR, creating new opportunities in materials science, structural biology, and metabolomics studies. Since protein NMR spectra recorded under DNP conditions can show improved spectral resolution at 180-200 K compared to 110 K, we investigate the effects of AMUPol and various deuterated TOTAPOL isotopologues on sensitivity and spectral resolution at these temperatures, using proline and reproducibly prepared SH3 domain samples...
October 28, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27783974/synthesis-of-new-donepezil-analogues-and-investigation-of-their-effects-on-cholinesterase-enzymes
#13
Begüm Nurpelin Sağlık, Sinem Ilgın, Yusuf Özkay
Donepezil (DNP), an acetylcholinesterase (AChE) inhibitor, is one of the most preferred choices in Alzheimer diseases (AD) therapy. In the present study, 38 new DNP analogues were synthesized. Structures of the synthesized compounds (1-38) were elucidated by IR, (1)H NMR, (13)C NMR and HRMS spectroscopic methods and elemental analysis. Inhibitory potential of the compounds on cholinesterase enzymes was investigated. None of the compounds displayed significant activity on butyrylcholinesterase (BChE) enzyme...
October 19, 2016: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27782260/microwave-gated-dynamic-nuclear-polarization
#14
Aurélien Bornet, Arthur Pinon, Aditya Jhajharia, Mathieu Baudin, Xiao Ji, Lyndon Emsley, Geoffrey Bodenhausen, Jan Henrik Ardenkjaer-Larsen, Sami Jannin
Dissolution dynamic nuclear polarization (D-DNP) has become a method of choice to enhance signals in nuclear magnetic resonance (NMR). Recently, we have proposed to combine cross-polarization (CP) with D-DNP to provide high polarization P((13)C) in short build-up times. In this paper, we show that switching microwave irradiation off for a few hundreds of milliseconds prior to CP can significantly boost the efficiency. By implementing microwave gating, (13)C polarizations on sodium [1-(13)C]acetate as high as 64% could be achieved with a polarization build-up time constant as short as 160 s...
October 26, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27780134/inhibitory-effects-of-norlignans-isolated-from-anemarrhena-asphodeloides-on-degranulation-of-rat-basophilic-leukemia-%C3%A2-2h3cells
#15
Jong Phil Bak, Young Mi Cho, Inhye Kim, Dae Won Park, Jung Eun Kwon, Yong Joon Jeong, Jong Hwan Kwak, Se Chan Kang
Anemarrhena asphodeloides is known to suppress inflammation and lower various fevers. To determine the active component of A. asphodeloides, ethanol (EtOH) extract of A. asphodeloides rhizomes was fractionized. The compounds isolated from the dichloromethane (CH2Cl2) soluble fraction were identified as 4'-O-methylnyasol (1), nyasol (2), 3″-methoxynyasol (3), 3″-hydroxy-4″-methoxy-4″-dehydroxynyasol (4), 4-hydroxybenzaldehyde (5), and 4-hydroxyacetophenone (6). The four norlignans (1-4) potently inhibited the release of β-hexosaminidase from immunoglobulin E (IgE)/dinitrophenol-conjugated bovine serum albumin (DNP-BSA)-treated rat basophilic leukemia (RBL)-2H3 and A23187 plus phorbol 12-myristate 13-acetate co-treated isolated rat primary mast cells, as markers of degranulation and histamine release...
October 22, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27777798/mentoring-nurses-in-political-skill-to-navigate-organizational-politics
#16
Wanda Montalvo, Mary W Byrne
Objective. The aim of this study was to describe and analyze the correlations between mentoring functions and political skill development among nurses who have earned or are candidates for a Ph.D. or doctorate of nursing practice (DNP) degree. Background. The healthcare system is in flux; future generations of Ph.D. and DNP nurse leaders will be required to demonstrate political acumen. Political skill to navigate organizational politics has had limited research within nursing. Methods. A cross-sectional research design using a web-based survey of 222 nurses who have earned or are candidates for a Ph...
2016: Nursing Research and Practice
https://www.readbyqxmd.com/read/27775111/dynamic-nuclear-polarisation-by-thermal-mixing-quantum-theory-and-macroscopic-simulations
#17
Alexander Karabanov, Grzegorz Kwiatkowski, Carlo U Perotto, Daniel Wiśniewski, Jonathan McMaster, Igor Lesanovsky, Walter Köckenberger
A theory of dynamic nuclear polarisation (DNP) by thermal mixing is suggested based on purely quantum considerations. A minimal 6-level microscopic model is developed to test the theory and link it to the well-known thermodynamic model. Optimal conditions for the nuclear polarization enhancement and effects of inhomogeneous broadening of the electron resonance are discussed. Macroscopic simulations of nuclear polarization spectra displaying good agreement with experiments, involving BDPA and trityl free radicals, are presented...
October 24, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27766840/hyperpolarized-multi-metal-13-c-sensors-for-mri
#18
Anurag Mishra, Giorgio Pariani, Thomas Oerther, Markus Schwaiger, Gil Gregor Westmeyer
We introduce hyperpolarizable (13)C-labelled probes that identify multiple biologically important divalent metals via metal-specific chemical shifts. These features enable NMR measurements of calcium concentrations in human serum in the presence of magnesium. In addition, signal enhancement through Dynamic Nuclear Polarization (DNP) increases the sensitivity of metal detection to afford measuring micromolar concentrations of calcium as well as simultaneous multi-metal detection by chemical shift imaging. The hyperpolarizable (13)C-MRI sensors presented here enable sensitive NMR measurements and MR imaging of multiple divalent metals in opaque biological samples...
October 21, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27749604/sirt1-attenuates-neuropathic-pain-by-epigenetic-regulation-of-mglur1-5-expressions-in-type-2-diabetic-rats
#19
Cheng-Hua Zhou, Ming-Xing Zhang, Sha-Sha Zhou, Huan Li, Jian Gao, Lei Du, Xiao-Xing Yin
Accumulating evidence has demonstrated that epigenetic modification-mediated changes in pain-related gene expressions play an important role in the development and maintenance of neuropathic pain. Sirtuin 1 (SIRT1), anicotinamide adenosine dinucleotide (NAD)-dependent deacetylase, is involved in the development of chronic pain. Moreover, SIRT1 may be a novel therapeutic target for the prevention of type 2 diabetes mellitus (T2DM). But the role of SIRT1 in T2DM-induced neuropathic pain remains unknown. In this study, we found that spinal SIRT1 expression and activity were down-regulated significantly in high-fat-fed/low-dose STZ-induced neuropathic pain rats...
September 29, 2016: Pain
https://www.readbyqxmd.com/read/27743654/impacts-of-detrital-nano-and-micro-scale-particles-dnp-on-contaminant-dynamics-in-a-coal-mine-amd-treatment-system
#20
Liliana Lefticariu, Stephen R Sutton, Kelly S Bender, Mihai Lefticariu, Martin Pentrak, Joseph W Stucki
Pollutants in acid mine drainage (AMD) are usually sequestered in neoformed nano- and micro-scale particles (nNP) through precipitation, co-precipitation, and sorption. Subsequent biogeochemical processes may control nNP stability and thus long-term contaminant immobilization. Mineralogical, chemical, and microbiological data collected from sediments accumulated over a six-year period in a coal-mine AMD treatment system were used to identify the pathways of contaminant dynamics. We present evidence that detrital nano- and micron-scale particles (dNP), composed mostly of clay minerals originating from the partial weathering of coal-mine waste, mediated biogeochemical processes that catalyzed AMD contaminant (1) immobilization by facilitating heterogeneous nucleation and growth of nNP in oxic zones, and (2) remobilization by promoting phase transformation and reductive dissolution of nNP in anoxic zones...
October 13, 2016: Science of the Total Environment
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