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Joseph H Y Galani, Jalpesh S Patel, Nilesh J Patel, Jayant G Talati
It is of paramount importance for consumers, scientists and industrialists to understand how low-temperature storage of food items affects their bioactive compounds and properties. This study evaluated the effects of cold storage on total phenolics (TP), phenolic acids profile (PA), total anthocyanins (TA), total ascorbic acid (Vit. C) and antioxidant activity (AA) of 19 fruits and vegetables, collected from local Indian markets and stored in refrigerator (4 °C) during 15 days. Content of TP was highest in dill and amaranth and decreased (up to 29...
July 24, 2017: Antioxidants (Basel, Switzerland)
Christian Hundshammer, Stephan Düwel, Simone Köcher, Malte Gersch, Benedikt Feuerecker, Christoph Scheurer, Axel Haase, Steffen J Glaser, Markus Schwaiger, Franz Schilling
Aberrant pH is characteristic of many pathologies such as ischemia, inflammation or cancer. Therefore, a non-invasive and spatially resolved pH determination is valuable for disease diagnosis, characterization of response to treatment and the design of pH-sensitive drug delivery systems. We recently introduced hyperpolarized zymonic acid (ZA) as a novel MRI probe of extracellular pH utilizing dissolution dynamic polarization (DNP) for a more than 10,000-fold NMR signal enhancement. Here we present a strategy to enhance the sensitivity of this approach by deuteration of ZA (ZAd)...
July 18, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
Mengxiao Liu, Yaewon Kim, Christian Hilty
Chemical exchange phenomena are ubiquitous in macromolecules, which undergo conformational change or ligand complexation. NMR relaxation dispersion (RD) spectroscopy based on a Carr-Purcell-Meiboom-Gill pulse sequence is widely applied to identify the exchange and measure the life time of intermediate states on the millisecond time scale. Advances in hyperpolarization methods improve the applicability of NMR spectroscopy when rapid acquisitions or low concentrations are required, through an increase in signal strength by several orders of magnitude...
July 17, 2017: Analytical Chemistry
Lu-Lu Shi, Wei-Jia Fan, Ji-Ying Zhang, Xiao-Ya Zhao, Song Tan, Jing Wen, Jing Cao, Xue-Ying Zhang, Qing-Sheng Chi, De-Hua Wang, Zhi-Jun Zhao
The metabolic thermogenesis plays important roles in thermoregulation, and it may be also involved in body fat regulation. The thermogenesis of brown adipose tissue (BAT) is largely affected by ambient temperature, but it is unclear if the roles in body fat regulation are dependent on the temperature. In the present study, uncoupling protein 1 (ucp1)-based BAT thermogenesis, energy budget and body fat content were examined in the striped hamsters fed high fat diet (HF) at cold (5°C) and warm (30°C) temperatures...
July 12, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
Jean-Nicolas Dumez, Basile Vuichoud, Daniele Mammoli, Aurélien Bornet, Arthur C Pinon, Gabriele Stevanato, Benno Meier, Geoffrey Bodenhausen, Sami Jannin, Malcolm H Levitt
We have induced hyperpolarized long-lived states in compounds containing 13C-bearing methyl groups by dynamic nuclear polarization (DNP) at cryogenic temperatures, followed by dissolution with a warm solvent. The hyperpolarized methyl long-lived states give rise to enhanced antiphase 13C NMR signals in solution, which often persist for times much longer than the 13C and 1H spin-lattice relaxation times under the same conditions. The DNP-induced effects are similar to quantum-rotor-induced-polarization (QRIP), but are observed in a wider range of compounds, since they do not depend critically on the height of the rotational barrier...
July 14, 2017: Journal of Physical Chemistry Letters
Fabian Jähnig, Grzegorz Kwiatkowski, Alexander Däpp, Andreas Hunkeler, Beat H Meier, Sebastian Kozerke, Matthias Ernst
Dissolution DNP has become an important method to generate highly polarized substrates such as pyruvic acid for in vivo imaging and localized spectroscopy. In a quest to further increase the polarization levels, which is important for in vivo MRI employing (13)C detection, we describe the design and implementation of a new DNP polarizer that is suitable for dissolution operation at 7 T static magnetic field and a temperature of 1.4 K. We describe all important sample preparation steps and experimental details necessary to optimize trityl based samples for use in our polarizer at this higher field...
July 26, 2017: Physical Chemistry Chemical Physics: PCCP
Keisuke Okamura, Tetsu Okuda, Kazuyuki Shirai, Hidenori Urata
The aim of the present study was to establish a convenient clinically applicable assay method for chymase-dependent angiotensin II forming activity of circulating mononuclear leukocytes (CML), which was potentially a marker of tissue chymase activity. Using this method, association between CML chymase activity and clinical parameters was determined. Cardiovascular outpatients (n = 170) without taking antihypertensive medication were recruited. An ELISA for chymase-dependent angiotensin II-forming activity in CML was established using Nma /Dnp-modified angiotensin I...
July 12, 2017: Clinical and Experimental Hypertension: CHE
Andi Marquardt, Moh'd Mohanad Al-Dabet, Sanchita Ghosh, Shrey Kohli, Jayakumar Manoharan, Ahmed ElWakiel, Ihsan Gadi, Fabian Bock, Sumra Nazir, Hongjie Wang, Jonathan A Lindquist, Peter Paul Nawroth, Thati Madhusudhan, Peter R Mertens, Khurrum Shahzad, Berend Isermann
Established therapies for diabetic nephropathy (dNP) delay but do not prevent its progression. The shortage of established therapies may reflect the inability to target the tubular compartment. The chemical chaperone tauroursodeoxycholic acid (TUDCA) ameliorates maladaptive endoplasmic reticulum (ER) stress signaling and experimental dNP. Additionally, TUDCA activates the farnesoid X receptor (FXR), which is highly expressed in tubular cells. We hypothesized that TUDCA ameliorates maladaptive ER signaling via FXR agonism specifically in tubular cells...
July 10, 2017: Journal of the American Society of Nephrology: JASN
Sachin R Chaudhari, Dorothea Wisser, Arthur C Pinon, Pierrick Berruyer, David Gajan, Paul Tordo, Olivier Ouari, Christian Reiter, Frank Engelke, Christophe Copéret, Moreno Lelli, Anne Lesage, Lyndon Emsley
Dynamic nuclear polarization (DNP) has recently emerged as a tool to enhance the sensitivity of solid-state NMR experiments. However, so far high enhancements (>100) are limited to relatively low magnetic fields, and DNP at fields higher than 9.4 T significantly drops in efficiency. Here we report solid-state Overhauser effect DNP enhancements of over 100 at 18.8 T. This is achieved through the unexpected discovery that enhancements increase rapidly with increasing magic angle spinning (MAS) rates. The measurements are made using 1,3-bisdiphenylene-2-phenylallyl dissolved in o-terphenyl at 40 kHz MAS...
July 27, 2017: Journal of the American Chemical Society
Frédéric Blanc
Observation of Brønsted acid sites: By enhancing the solid-state NMR signals of (17) O at natural abundance with dynamic nuclear polarization (DNP), Pruski et al. were able to measure oxygen-proton distances accurately with sub-picometer precision. The results give insight into the Brønsted acidity of a range of solid acid catalysts.
July 10, 2017: Angewandte Chemie
Mohammad Wahab Khan, Ayaluru Murali
Alcohol oxidase (AOX) is an important flavin adenine dinucleotide (FAD) dependent oxidoreductase, which is responsible for converting methanol into formaldehyde and hydrogen peroxide for the growth of methylotrophic yeast Candida boidinii. Although AOX plays a crucial role in methanol catabolism, the experimental structure of AOX from Candida boidinii has not been elucidated. This study reports the first complete in silico model of AOX from C. boidinii. This paper also reports the AOX structure modeled using the threading approach, followed by structure analysis and molecular dynamics simulation...
July 10, 2017: Molecular BioSystems
Pongthep Prajongtat, Songwut Suramitr, Somkiat Nokbin, Koichi Nakajima, Koichiro Mitsuke, Supa Hannongbua
Structural and electronic properties of eight isolated azo dyes (ArNNAr', where Ar and Ar' denote the aryl groups containing benzene and naphthalene skeletons, respectively) were investigated by density functional theory (DFT) based on the B3LYP/6-31G(d,p) and TD-B3LYP/6-311G(d,p) methods The effect of methanol solvent on the structural and electronic properties of the azo dyes was elucidated by employing a polarizable continuum model (PCM). Then, the azo dyes adsorbed onto the anatase TiO2 (101) slab surface through a carboxyl group...
June 11, 2017: Journal of Molecular Graphics & Modelling
Koen Visscher, João Medeiros-Silva, Deni Mance, João P G L M Rodrigues, Mark Daniëls, Alexandre M J J Bonvin, Marc Baldus, Markus Weingarth
The segregation of cellular surfaces in heterogeneous patches is considered a common motif in bacteria and eukaryotes, which is underpinned by the observation of clustering and cooperative gating of signalling membrane proteins like receptors or channels. Such processes could represent an important cellular strategy to shape signalling activity. Hence, structural knowledge of the arrangement of channels or receptors in supramolecular assemblies represents a crucial step towards a better understanding of signalling across membranes...
July 7, 2017: Angewandte Chemie
Reas S Khan, Kimberly Dine, John G Geisler, Kenneth S Shindler
The ability of novel mitochondrial uncoupler prodrug of 2,4-dinitrophenol (DNP), MP201, to prevent neuronal damage and preserve visual function in an experimental autoimmune encephalomyelitis (EAE) model of optic neuritis was evaluated. Optic nerve inflammation, demyelination, and axonal loss are prominent features of optic neuritis, an inflammatory optic neuropathy often associated with the central nervous system demyelinating disease multiple sclerosis. Currently, optic neuritis is frequently treated with high-dose corticosteroids, but treatment fails to prevent permanent neuronal damage and associated vision changes that occur as optic neuritis resolves, thus suggesting that additional therapies are required...
2017: Oxidative Medicine and Cellular Longevity
Brenda L Tyczkowski, Janet Reilly
It is no secret that there is a nursing faculty shortage due to aging faculty, retention issues, and an increased need for more nurses with the DNP degree. This parallels the ongoing nursing shortage, as lack of faculty limits nursing student capacity. Explanation of how DNP-prepared faculty can help fill the nursing faculty shortage and strategies are explored in this article.
July 2017: Journal of Nursing Administration
Yuefei Ji, Lu Wang, Mengdi Jiang, Junhe Lu, Corinne Ferronato, Jean-Marc Chovelon
As promising in-situ chemical oxidation (ISCO) technologies, sulfate radical-based advanced oxidation processes (SR-AOPs) are applied in wastewater treatment and groundwater remediation in recent years. In this contribution, we report for the first time that, thermally activated persulfate oxidation of phenol in the presence of nitrite (NO2(-)), an anion widely present in natural waters, could lead to the formation of nitrated by-products including 2-nitrophenol (2-NP), 4-nitrophenol (4-NP), 2,4-dinitrophenol (2,4-DNP), and 2,6-dinitrophenol (2,6-DNP)...
June 28, 2017: Water Research
Shea Polancich, David H James, Rebecca S Miltner, Glenda L Smith, Linda Moneyham
Effectively teaching clinical data management and analysis in a doctor of nursing (DNP) program requires attention to developing skills that foster improvement at the bedside. The purpose of this article is to describe a DNP clinical data management and analysis course specific to translation and improvement in practice. The student evaluation scores are high; however, faculty evaluation identifies opportunities to improve translation and application at the point of the scholarly practice project.
June 29, 2017: Nurse Educator
Arnab Dey, Abhishek Banerjee, Narayanan Chandrakumar
Overhauser dynamic nuclear polarization (ODNP) is investigated at a moderately low field (1.2 T) for natural abundance (13)C NMR of small molecules in solution state at room temperature. It is shown that ODNP transferred from (1)H to (13)C by NMR coherence transfer is in general significantly more efficient than direct ODNP of (13)C. Compared to direct (13)C ODNP, we demonstrate over 4-fold higher (13)C sensitivity (signal-to-noise ratio, SNR), achieved in one-eighth of the measurement time by transferred ODNP (t-ODNP)...
July 27, 2017: Journal of Physical Chemistry. B
Christophe Copéret, Wei-Chih Liao, Christopher P Gordon, Ta-Chung Ong
Development of well-defined heterogeneous catalysts requires detailed structural characterization of active sites, an essential step toward establishing structure-activity relationships and promoting rational designs of catalysts. Solid-state NMR has emerged as a powerful approach to provide key molecular-level information about active-site structures and dynamics in heterogeneous catalysis. Here, we describe how one can apply solid-state NMR, ranging from 1D chemical shift assignments (and additional parameters, CQ and η, for quadrupolar nuclei, I > (1)/2) to 2D correlations, to analysis of chemical shift anisotropy, providing unprecedented structural information about a broad range of materials...
July 17, 2017: Journal of the American Chemical Society
Mohamed Fahmy Abou Elazab, Yoshiaki Inoue, Hisakazu Kamei, Hiroyuki Horiuchi, Shuichi Furusawa
Vaccination is the most effective method for controlling the infectious diseases that threaten the poultry industry worldwide. The use of adjuvants or immunostimulants is often necessary to improve vaccine efficacy, particularly for vaccines based on recombinant protein or inactivated pathogens. The adjuvant effects of zymosan A on antigen-specific antibody production were investigated in chickens. First, the optimal adjuvant dose of zymosan A was determined. Chicks were immunized with dinitrophenyl-keyhole limpet hemocyanin (DNP-KLH) at a dosage of 2 mg/kg body weight (BW) with or without zymosan A (at a dosage of 0...
June 23, 2017: Journal of Veterinary Medical Science
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