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https://www.readbyqxmd.com/read/28646173/transmembrane-interactions-of-full-length-mammalian-bitopic-cytochrome-p450-cytochrome-b5-complex-in-lipid-bilayers-revealed-by-sensitivity-enhanced-dynamic-nuclear-polarization-solid-state-nmr-spectroscopy
#1
Kazutoshi Yamamoto, Marc A Caporini, Sang-Choul Im, Lucy Waskell, Ayyalusamy Ramamoorthy
The dynamic protein-protein and protein-ligand interactions of integral bitopic membrane proteins with a single membrane-spanning helix play a plethora of vital roles in the cellular processes associated with human health and diseases, including signaling and enzymatic catalysis. While an increasing number of high-resolution structural studies of membrane proteins have successfully manifested an in-depth understanding of their biological functions, intact membrane-bound bitopic protein-protein complexes pose tremendous challenges for structural studies by crystallography or solution NMR spectroscopy...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28631929/-13-c-dynamic-nuclear-polarization-using-a-trimeric-gd-3-complex-as-an-additive
#2
Peter Niedbalski, Christopher Parish, Qing Wang, Andhika Kiswandhi, Zahra Hayati, Likai Song, Lloyd Laporca Lumata
Dissolution dynamic nuclear polarization (DNP) is one of the most successful techniques that resolves the insensitivity problem in liquid-state nuclear magnetic resonance (NMR) spectroscopy and imaging (MRI) by amplifying the signal by several thousand-fold. One way to further improve the DNP signal is the inclusion of trace amounts of lanthanides in DNP samples doped with trityl OX063 free radical as the polarizing agent. In practice, stable monomeric gadolinium complexes such as Gd-DOTA or Gd-HP-DO3A are used as beneficial additives in DNP samples, further boosting the DNP-enhanced solid-state 13C polarization by a factor of 2 or 3...
June 20, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28626198/the-therapeutic-effect-of-dexmedetomidine-on-rat-diabetic-neuropathy-pain-and-the-mechanism
#3
Yuecheng Lu, Baohua Lin, Junmin Zhong
Diabetic neuropathy pain (DNP) is a common chronic complication of diabetes characterized by spontaneous pain, hyperalgesia and allodynia. Dexmedetomidine is a selective α2 adrenergic agonist that relieves sympathetic nervous tension and reduces the release of glutamate. Thus, it is possible that dexmedetomidine may relieve DNP as well. In this study, we examined the effect of dexmedetomidine on DNP in the presence or absence of the α2 adrenergic antagonist yohimbine in rats utilizing a streptozotocin (STZ)-induced diabetes as a model of DNP...
June 14, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28623034/new-nmr-tools-for-protein-structure-and-function-spin-tags-for-dynamic-nuclear-polarization-solid-state-nmr
#4
Rivkah Rogawski, Ann McDermott
Magic angle spinning solid state NMR studies of biological macromolecules [1-3] have enabled exciting studies of membrane proteins [4,5], amyloid fibrils [6], viruses, and large macromolecular assemblies [7]. Dynamic nuclear polarization (DNP) provides a means to enhance detection sensitivity for NMR, particularly for solid state NMR, with many recent biological applications and considerable contemporary efforts towards elaboration and optimization of the DNP experiment. This review explores precedents and innovations in biological DNP experiments, especially highlighting novel chemical biology approaches to introduce the radicals that serve as a source of polarization in the DNP experiments...
June 13, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28619991/evidence-that-differentiation-inducing-factor-1-controls-chemotaxis-and-cell-differentiation-at-least-in-part-via-mitochondria-in-d-discoideum
#5
Yuzuru Kubohara, Haruhisa Kikuchi, Van Hai Nguyen, Hidekazu Kuwayama, Yoshiteru Oshima
Differentiation-inducing factor-1 [1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one (DIF-1)] is an important regulator of cell differentiation and chemotaxis in the development of the cellular slime mold Dictyostelium discoideum However, the entire signaling pathways downstream of DIF-1 remain to be elucidated. To characterize DIF-1 and its potential receptor(s), we synthesized two fluorescent derivatives of DIF-1, boron-dipyrromethene (BODIPY)-conjugated DIF-1 (DIF-1-BODIPY) and nitrobenzoxadiazole (NBD)-conjugated DIF-1 (DIF-1-NBD), and investigated their biological activities and cellular localization...
June 15, 2017: Biology Open
https://www.readbyqxmd.com/read/28619669/sex-pheromone-in-the-moth-heliothis-virescens-is-produced-as-a-mixture-of-two-pools-de-novo-and-via-precursor-storage-in-glycerolipids
#6
Stephen P Foster, Karin G Anderson, Jérôme Casas
Most species of moths use a female-produced volatile sex pheromone, typically produced via de novo fatty acid synthesis in a specialized gland, for communication among mates. While de novo biosynthesis of pheromone (DNP) is rapid, suggesting transient precursor acids, substantial amounts of pheromone precursor (and other) acids are stored, predominantly in triacylglycerols in the pheromone gland. Whether these stored acids are converted to pheromone later or not has been the subject of some debate. Using a tracer/tracee approach, in which we fed female Heliothis virescens U-(13)C-glucose, we were able to distinguish two pools of pheromone, in which precursors were temporally separated (after and before feeding on labeled glucose): DNP synthesized from a mixed tracer/tracee acetyl CoA pool after feeding, and pheromone made from precursor acids primarily synthesized before feeding, which we call recycled precursor fat pheromone (RPP)...
June 12, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28611402/dynamic-nuclear-polarization-nmr-as-a-new-tool-to-investigate-the-nature-of-organic-compounds-occluded-in-plant-silica-particles
#7
Armand Masion, Anne Alexandre, Fabio Ziarelli, Stéphane Viel, Guaciara M Santos
The determination of the chemical nature of the organic matter associated with phytoliths remains a challenge. This difficulty mainly stems from amounts of organic carbon (C) that are often well below the detection limit of traditional spectroscopic tools. Conventional solid-state (13)C Nuclear Magnetic Resonance (NMR) is widely used to examine the nature and structure of organic molecules, but its inherent low sensitivity prohibits the observation of diluted samples. The recent advent of commercial microwave source in the terahertz range triggered a renewed interest in the Dynamic Nuclear Polarization (DNP) technique to improve the signal to noise ratio of solid-state NMR experiments...
June 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28603465/spinal-cord-as-an-adjunct-to-brain-magnetic-resonance-imaging-in-defining-no-evidence-of-disease-activity-in-multiple-sclerosis
#8
Subhash Tummala, Tarun Singhal, Vinit V Oommen, Gloria Kim, Fariha Khalid, Brian C Healy, Rohit Bakshi
ACTIVITY AVAILABLE ONLINE: To access the article, post-test, and evaluation online, go to http://www.cmscscholar.org. TARGET AUDIENCE: The target audience for this activity is physicians, physician assistants, nursing professionals, and other health-care providers involved in the management of patients with multiple sclerosis (MS). LEARNING OBJECTIVES: 1) Describe the concept of "no evidence of disease activity" in MS.2) Recognize the role of spinal cord imaging in routine monitoring of MS disease activity...
May 2017: International Journal of MS Care
https://www.readbyqxmd.com/read/28599086/in-silico-design-of-dnp-polarizing-agents-can-current-dinitroxides-be-improved
#9
Frédéric A Perras, Aaron D Sadow, Marek Pruski
Numerical calculations of enhancement factors offered by dynamic nuclear polarization in solids under magic angle spinning (DNP-MAS) were performed to determine the optimal EPR parameters for a dinitroxide polarizing agent. We found that the DNP performance of a biradical is more tolerant to the relative orientation of the two nitroxide moieties than previously thought. Generally, any condition in which the gyy tensor components of both radicals are perpendicular to one another is expected to have near-optimal DNP performance...
June 9, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28599057/dynamic-nuclear-polarization-studies-of-nitroxyl-spin-probes-in-agarose-gel-using-overhauser-enhanced-magnetic-resonance-imaging-omri
#10
V Meenakumari, Hideo Utsumi, Fuminori Hyodo, A Jawahar, A Milton Franklin Benial
Agarose is a tissue-equivalent material and its imaging characteristics similar to those of real tissues. Hence the dynamic nuclear polarization studies of carboxy-PROXYL in agarose gel were carried out. The DNP parameters such as spin lattice relaxation time, longitudinal relaxivity, leakage factor, saturation parameter and coupling parameter were estimated for 2 mM carboxy-PROXYL in PBS solution and water/agarose mixture (99:1). From these results, the spin probe concentration was optimized as 2 mM, and the reduction in enhancement was observed for carboxy-PROXYL in water/agarose mixture (99:1) compared with PBS solution...
June 9, 2017: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/28598552/acrolein-scavenger-hydralazine-prevents-streptozotocin-induced-painful-diabetic-neuropathy-and-spinal-neuroinflammation-in-rats
#11
Lu Yao, Yun-Tao Wu, Guo-Xiang Tian, Chang-Quan Xia, Feng Zhang, Wei Zhang
Diabetes-induced neuropathic pain (DNP) substantially influences people's life qualities. Hyperglycemia-induced excess free radicals have been considered as the most critical mechanisms underlying DNP. As an unsaturated aldehyde and a reactive product of lipid peroxidation, acrolein plays critical roles in diabetic nephropathy and inflammatory pain. We sought to determine whether acrolein is involved in DNP in the present study. Diabetes was induced by a single intraperitoneal (i.p.) injection of 60 mg/kg streptozotocin (STZ)...
June 9, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28598474/anisotropic-longitudinal-electronic-relaxation-affects-dnp-at-cryogenic-temperatures
#12
E M M Weber, H Vezin, J G Kempf, G Bodenhausen, D Abergél, D Kurzbach
We report the observation of anisotropic longitudinal electronic relaxation in nitroxide radicals under typical dynamic nuclear polarization conditions. This anisotropy affects the efficiency of dynamic nuclear polarization at cryogenic temperatures of 4 K and high magnetic fields of 6.7 T. Under our experimental conditions, the electron paramagnetic resonance spectrum of nitroxides such as TEMPOL (4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl) is only partly averaged by electronic spectral diffusion, so that the relaxation times T1e(ω) vary across the spectrum...
June 21, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28593642/assembly-and-performance-of-a-6-4-t-cryogen-free-dynamic-nuclear-polarization-system
#13
Andhika Kiswandhi, Peter Niedbalski, Christopher Parish, Qing Wang, Lloyd Lumata
We report on the assembly and performance evaluation of a 180 GHz/6.4 T dynamic nuclear polarization (DNP) system based on a cryogen-free superconducting magnet. The DNP system utilizes a variable-field superconducting magnet that can be ramped up to 9 T and equipped with cryocoolers that can cool the sample space with the DNP assembly down to 1.8 K via the Joule-Thomson effect. A homebuilt DNP probe insert with top-tuned NMR coil and microwave port was incorporated into the sample space in which the effective sample temperature is approximately 1...
June 7, 2017: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/28587350/effects-of-hyperbaric-factors-on-lidocaine-induced-apoptosis-in-spinal-neurons-and-the-role-of-p38-mitogen-activated-protein-kinase-in-rats-with-diabetic-neuropathic-pain
#14
Xiaolan Zheng, Ling Chen, Xiaohong Du, Junying Cai, Shuchun Yu, Hongtao Wang, Guohai Xu, Zhenzhong Luo
The application of lidocaine can lead to nerve damage. Evidence suggests that patients with diabetic neuropathy are at a higher risk for neurotoxicity. In the present study, the successful induction of diabetic neuropathic pain (DNP) in rats via a high-sugar, high-fat diet and intraperitoneal injection of 1% streptozotocin was verified and pronounced tactile allodynia was observed. It was found that intrathecal injections of hyperbaric lidocaine produced motor blocks of longer durations in the DNP model rats than in nondiabetic rats, or in DNP model rats injected with isobaric lidocaine...
June 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28585986/evaluation-of-doctor-of-nursing-practice-students-competencies-in-an-online-informatics-course
#15
Barbara Kupferschmid, Connie Creech, Marsha Lesley, Marilyn Filter, Christina Aplin-Kalisz
BACKGROUND: Doctor of Nursing Practice (DNP) programs have experienced rapid growth across the United States. With the expansion of electronic health records, DNP students are expected to acquire the knowledge and skills needed to manage data and apply informatics concepts. However, little information exists to help faculty evaluate student competency in these areas. METHOD: This retrospective analysis evaluated the competencies of a convenience sample of DNP students enrolled in an online informatics course...
June 1, 2017: Journal of Nursing Education
https://www.readbyqxmd.com/read/28585130/factors-associated-with-peripheral-neuropathy-in-type-2-diabetes-subclinical-versus-confirmed-neuropathy
#16
Yogendranathsing Unmar, Mohammad Ishraq Zafar, Feng Gao
This study determined the prevalence of diabetic peripheral neuropathy (DPN) and subclinical DPN (sDPN) in patients with type 2 diabetes mellitus (T2DM) using nerve conduction study (NCS) as a diagnostic tool. We also investigated the factors associated with the development of sDPN and compared factors between the sDPN and confirmed DPN (cDPN). This cross-sectional study involved 240 T2DM patients who were successively admitted to the endocrinology wards of Wuhan Union Hospital over the period of January to December 2014...
June 2017: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/28582614/local-structures-and-heterogeneity-of-silica-supported-m-iii-sites-evidenced-by-epr-ir-nmr-and-luminescence-spectroscopies
#17
Murielle F Delley, Giuseppe Lapadula, Francisco Núñez-Zarur, Aleix Comas-Vives, Vidmantas Kalendra, Gunnar Jeschke, Dirk Baabe, Marc D Walter, Aaron J Rossini, Anne Lesage, Lyndon Emsley, Olivier Maury, Christophe Copéret
Grafting molecular precursors on partially dehydroxylated silica followed by a thermal treatment yields silica-supported M(III) sites for a broad range of metals. They display unique properties such as high activity in olefin polymerization and alkane dehydrogenation (M = Cr) or efficient luminescence properties (M = Yb and Eu) essential for bioimaging. Here, we interrogate the local structure of the M(III) surface sites obtained from two molecular precursors, amides M(N(SiMe3)2)3 vs siloxides (M(OSi(O(t)Bu)3)3·L with L = (THF)2 or HOSi(O(t)Bu)3 for M = Cr, Yb, Eu, and Y, by a combination of advanced spectroscopic techniques (EPR, IR, XAS, UV-vis, NMR, luminescence spectroscopies)...
June 21, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28577812/re-envisioning-a-dnp-program-for-quality-and-sustainability
#18
Marcia Killien, Hilaire Thompson, Gail Kieckhefer, Betty Bekemeier, Yoriko Kozuki, Cynthia K Perry
When the University of Washington, School of Nursing determined that its post-BSN-DNP degree program, with multiple specialty tracks and programs of study, was not sustainable, the curriculum was re-envisioned. The revised program is consistent with the American Association of Colleges of Nursing (AACN) Essentials of Doctoral Education for Advanced Nursing Practice and the national Licensure Accreditation, Certification, and Education (LACE) model. The re-envisioned program was conceptualized as a single degree in which students preparing for any specialty would have the same number of required credits with the majority of courses (DNP core) required for all students...
May 2017: Journal of Professional Nursing: Official Journal of the American Association of Colleges of Nursing
https://www.readbyqxmd.com/read/28575797/prediction-of-crystal-morphology-of-3-4-dinitro-1h-pyrazole-dnp-in-different-solvents
#19
Liang Song, Lizhen Chen, Jianlong Wang, Feng Chen, Guanchao Lan
Analytical grade ethyl acetate was used to recrystallize 3,4-Dinitro-1H-pyrazole (DNP), it was found that there were two different morphologies of the crystals. There is a possibility that ethyl acetate undergoes hydrolysis due to the absorption of moisture in the air. The influence of the hydrolysis products of acetic acid and ethanol on the morphology of DNP crystal was considered. In order to investigate the effect of solvents on DNP morphology, there is ongoing research to validate molecular dynamics (MD) simulation results with experiment data...
March 23, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28575508/effects-of-duloxetine-on-the-toll-like-receptor-4-signaling-pathway-in-spinal-dorsal-horn-in-a-rat-model-of-diabetic-neuropathic-pain
#20
Dong-Mei Zhou, Ying Zhuang, Wen-Jun Chen, Wei Li, Bei Miao
Objective. : Although duloxetine has been approved for clinical therapy for diabetic peripheral neuropathic pain, the exact mechanism underlying the anti-allodynic effects in rat models of diabetes mellitus remains obscure. We attempted to identify whether duloxetine exerts anti-allodynic effects via inhibition of the TLR4-Myd88-dependent pathway in diabetic neuropathic pain (DNP) rats. Methods. : An animal model of type 1 diabetic neuropathic pain was induced by intraperitoneal streptozotocin in 108 rats randomized into four groups: control, DNP, solvent control + DNP, and DNP + duloxetine...
May 29, 2017: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
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