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Nitzan Rimon-Dahari, Lia Heinemann-Yerushalmi, Ron Hadas, Lital Kalich-Philosoph, Dafna Ketter, Nava Nevo, Dalia Galiani, Nava Dekel
Members of the TGF-β superfamily take part in the control of folliculogenesis. Vasorin (Vasn) is a newly identified negative regulator of TGF-β signaling whose possible involvement in ovarian physiology has never been studied. Here, we demonstrate that Vasn is expressed in the ovary by somatic cells of follicles, and that its expression is up-regulated by LH. We established a conditional knockout (cKO) mouse model in which Vasn is deleted specifically in granulosa cells of growing follicles from the secondary stage onwards...
April 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Jianghong Man, Xingjiang Yu, Haidong Huang, Wenchao Zhou, Chaomei Xiang, Haohao Huang, Lucio Miele, Zhenggang Liu, Gurkan Bebek, Shideng Bao, Jennifer S Yu
Tumor hypoxia is associated with poor patient survival and is a characteristic of glioblastoma. Notch signaling is implicated in maintaining glioma stem-like cells (GSCs) within the hypoxic niche, although the molecular mechanisms linking hypoxia to Notch activation have not been clearly delineated. Here we show that Vasorin is a critical link between hypoxia and Notch signaling in GSCs. Vasorin is preferentially induced in GSCs by a HIF1α/STAT3 co-activator complex and stabilizes Notch1 protein at the cell membrane...
January 4, 2018: Cell Stem Cell
Kirim Kim, Mijeong Jeon, Hyo-Seol Lee, Jung-Chul Park, Seok-Jun Moon, Seong-Oh Kim, Sung-Won Cho, Je Seon Song
OBJECTIVE: Studies of regenerative therapies have focused on the paracrine effects of mesenchymal stem cells, but little has been revealed about the humoral factors of periodontal ligament (PDL) stem cells. The aim of this study was to identify and compare the secretory factors of human permanent- and deciduous-teeth PDL cells (P-PDL and D-PDL cells, respectively) in order to understand the characteristics of these cells and their potential applications in regenerative therapies. DESIGN: Conditioned media were collected from P-PDL and D-PDL cells (P-PDL-CM and D-PDL-CM, respectively)...
November 2016: Archives of Oral Biology
Paolo Panza, Austen A Sitko, Hans-Martin Maischein, Iris Koch, Matthias Flötenmeyer, Gavin J Wright, Kenji Mandai, Carol A Mason, Christian Söllner
BACKGROUND: In the visual system of most binocular vertebrates, the axons of retinal ganglion cells (RGCs) diverge at the diencephalic midline and extend to targets on both ipsi- and contralateral sides of the brain. While a molecular mechanism explaining ipsilateral guidance decisions has been characterized, less is known of how RGC axons cross the midline. RESULTS: Here, we took advantage of the zebrafish, in which all RGC axons project contralaterally at the optic chiasm, to characterize Islr2 as an RGC receptor required for complete retinal axon midline crossing...
October 22, 2015: Neural Development
Aixue Huang, Jie Dong, Shaohua Li, Chaonan Wang, Hongmei Ding, Hui Li, Xueting Su, Xingfeng Ge, Leqiao Sun, Chenjun Bai, Xuelian Shen, Tao Fang, Jie Li, Ningsheng Shao
Vasorin (VASN) is a type I transmembrane protein that plays important roles in tumor development and vasculogenesis. In this paper, we showed that VASN could be a key mediator of communication between tumor cells and endothelial cells. We confirmed for the first time that HepG2-derived VASN can be transferred to human umbilical vein endothelial cells (HUVECs) via receptor mediated endocytosis of exosomes, at least in part through HSPGs. The HepG2-derived VASN containing exosomes promote migration of recipient HUVECs cells...
2015: International Journal of Biological Sciences
Shiva Samavat, Shiva Kalantari, Mohsen Nafar, Dorothea Rutishauser, Mostafa Rezaei-Tavirani, Mahmoud Parvin, Roman A Zubarev
INTRODUCTION: Immunoglobulin A (IgA) nephropathy, the most common type of glomerulonephritis, is only diagnosed by invasive kidney biopsy. Urine proteome panel might help in noninvasive diagnosis and also better understanding of pathogenesis of IgA nephropathy. MATERIALS AND METHODS: Second mid-stream urine samples of 13 patients with biopsy-proven IgA nephropathy and 8 healthy controls were investigated by means of nanoscale liquid chromatography tandem mass spectrometry...
May 2015: Iranian Journal of Kidney Diseases
Shaohua Li, Hui Li, Xiqin Yang, Wei Wang, Aixue Huang, Jie Li, Xingliang Qin, Fei Li, Guanyi Lu, Hongmei Ding, Xueting Su, Lvbin Hou, Wei Xia, Ming Shi, Hongwen Zhang, Qiang Zhao, Jie Dong, Xingfeng Ge, Leqiao Sun, Chenjun Bai, Chaonan Wang, Xuelian Shen, Tao Fang, Fusheng Wang, Heqiu Zhang, Ningsheng Shao
UNLABELLED: We report a new biomarker of hepatocarcinoma, vasorin (VASN), screened by a subtractive EMSA-SELEX strategy from AFP negative serum of hepatocellular carcinoma (HCC) patients with extrahepatic metastases. VASN was verified to be highly expressed in sera of 100 cases of HCC patients compared with 97 cases of normal persons and 129 cases of hepatitis patients. Further validation by Q-PCR,IFA and Western blot showed higher expression of VASN at mRNA and protein levels in HCC cell lines and HCC tissues than in normal controls...
April 30, 2015: Oncotarget
Lavanya Balakrishnan, Raja Sekhar Nirujogi, Sartaj Ahmad, Mitali Bhattacharjee, Srikanth S Manda, Santosh Renuse, Dhanashree S Kelkar, Yashwanth Subbannayya, Rajesh Raju, Renu Goel, Joji Kurian Thomas, Navjyot Kaur, Mukesh Dhillon, Shantal Gupta Tankala, Ramesh Jois, Vivek Vasdev, Yl Ramachandra, Nandini A Sahasrabuddhe, Ts Keshava Prasad, Sujatha Mohan, Harsha Gowda, Subramanian Shankar, Akhilesh Pandey
BACKGROUND: Osteoarthritis is a chronic musculoskeletal disorder characterized mainly by progressive degradation of the hyaline cartilage. Patients with osteoarthritis often postpone seeking medical help, which results in the diagnosis being made at an advanced stage of cartilage destruction. Sustained efforts are needed to identify specific markers that might help in early diagnosis, monitoring disease progression and in improving therapeutic outcomes. We employed a multipronged proteomic approach, which included multiple fractionation strategies followed by high resolution mass spectrometry analysis to explore the proteome of synovial fluid obtained from osteoarthritis patients...
2014: Clinical Proteomics
Håvard Loftheim, Karsten Midtvedt, Anders Hartmann, Anna V Reisæter, Pål Falck, Hallvard Holdaas, Trond Jenssen, Leon Reubsaet, Anders Asberg
UNLABELLED: HASH(0x47a3610) BACKGROUND: Acute rejection (AR) episodes in renal transplant recipients are suspected when plasma creatinine is elevated and other potential causes out ruled. Graft biopsies are however needed for definite diagnosis. Non-invasive AR-biomarkers is an unmet clinical need. The urinary proteome is an interesting source in the search for such a biomarker in this population. METHODS: In this proof of principle study, serial urine samples in the early post transplant phase from 6 patients with biopsy verified acute rejections and 6 age-matched controls without clinical signs of rejection were analyzed by shotgun proteomics...
August 31, 2012: Transplantation Research
A Michaela Krautzberger, Barbara Kosiol, Manuela Scholze, Heinrich Schrewe
The murine vasorin (Vasn) gene, initially known as Slit-like 2, encodes a transmembrane protein that shares structural similarities with the eponymous Slit proteins. However, whether it also shares functional similarities with these large secreted proteins remains to be elucidated. Here, we report expression of Vasn during embryonic and fetal development of the mouse using whole-mount in situ hybridization (WISH) and histochemical detection of β-galactosidase expressed from a targeted Vasn(lacZ) knock-in allele...
May 2012: Gene Expression Patterns: GEP
Pyong-Gon Moon, Jeong-Eun Lee, Sungyong You, Taek-Kyun Kim, Ji-Hoon Cho, In-San Kim, Tae-Hwan Kwon, Chan-Duck Kim, Sun-Hee Park, Daehee Hwang, Yong-Lim Kim, Moon-Chang Baek
To identify biomarker candidates associated with early IgA nephropathy (IgAN) and thin basement membrane nephropathy (TBMN), the most common causes presenting isolated hematuria in childhood, a proteomic approach of urinary exosomes from early IgAN and TBMN patients was introduced. The proteomic results from the patients were compared with a normal group to understand the pathophysiological processes associated with these diseases at the protein level. The urinary exosomes, which reflect pathophysiological processes, collected from three groups of young adults (early IgAN, TBMN, and normal) were trypsin-digested using a gel-assisted protocol, and quantified by label-free LC-MS/MS, using an MS(E) mode...
June 2011: Proteomics
J Malapeira, C Esselens, J J Bech-Serra, F Canals, J Arribas
The activity of a variety of extracellular signaling factors is tightly regulated by proteins containing A Disintegrin And a Metalloprotease domain (ADAM) metalloproteases through limited proteolysis. Thus, the identification of ADAM substrates may unveil novel components and mechanisms of cell signaling pathways. We report the identification of the transmembrane protein vasorin (VASN), a transforming growth factor-β (TGFβ) trap, as a substrate of ADAM17. The metalloprotease efficiently generates a soluble fragment encompassing the extracellular domain of VASN...
April 21, 2011: Oncogene
Li Chen, Ji-hua Yao, Shu-hong Zhang, Lu Wang, Huai-dong Song, Jing-lun Xue
Nervous and vascular systems grow as parallel networks, indicating common cues in distal targets. We have identified a novel zebrafish gene slit-like 2 (slitl2) that might involve in zebrafish central neural and vascular morphogenesis. Whole-mount in situ hybridization of zebrafish embryo detected distinct signals of slitl2 transcripts in zebrafish midline structure of central nervous system similar to that of slits. Strong expression is also observed in zebrafish vasculature. Zebrafish slitl2 shares amino acid sequence identity of 41% with Homo sapiens slitl2 (vasorin) and Mus musculus slitl2, and 35%, 33% with Danio rerio slit3, slit2...
October 14, 2005: Biochemical and Biophysical Research Communications
Yuichi Ikeda, Yasushi Imai, Hidetoshi Kumagai, Tetsuya Nosaka, Yoshihiro Morikawa, Tomoko Hisaoka, Ichiro Manabe, Koji Maemura, Takashi Nakaoka, Takeshi Imamura, Kohei Miyazono, Issei Komuro, Ryozo Nagai, Toshio Kitamura
Growth factors, cell-surface receptors, adhesion molecules, and extracellular matrix proteins play critical roles in vascular pathophysiology by affecting growth, migration, differentiation, and survival of vascular cells. In a search for secreted and cell-surface molecules expressed in the cardiovascular system, by using a retrovirus-mediated signal sequence trap method, we isolated a cell-surface protein named vasorin. Vasorin is a typical type I membrane protein, containing tandem arrays of a characteristic leucine-rich repeat motif, an epidermal growth factor-like motif, and a fibronectin type III-like motif at the extracellular domain...
July 20, 2004: Proceedings of the National Academy of Sciences of the United States of America
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