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Adenosine metacholine

Agnes Afrodite Sumarelli Albuquerque, Marco Túlio Menezes Carvalho, Patricia Martinez Evora, Tales Rubens de Nadai, Andrea Carla Celotto, Paulo Roberto Barbosa Evora
The present text was motivated by the difficulties faced by our postgraduate students when using airways studies protocols and will take into consideration the three mechanisms of relaxation: (I) guanosine 3,5-cyclic monophosphate (cGMP)/NO-dependent; (II) adenosine 3,5-cyclic monophosphate (cAMP)/PGI2-dependent, and (III) hyperpolarization-dependent. Tracheal rings are studied in an organ bath containing a gassed physiological salt solution, usually at a temperature of 37 °C. An agent or procedure that causes contraction [acetylcholine (Ach) or metacholine] of the smooth muscle is needed before study airway dilator drugs...
June 2016: Annals of Translational Medicine
M Vischjager, T M Van Gulik, M Pfaffendorf, P A Van Zwieten, J Van Marle, J G Kromhout, P J Klopper, M J Jacobs
OBJECTIVES: To assess the function of arterial grafts after prolonged preservation in the University of Wisconsin solution (UW), in vitro and in vivo. METHODS: Carotid arteries were harvested from dogs and stored for 1-21 days at 4 degrees C in UW (n = 10) or in PBS (0.9% NaCl, pH 7.4), (PBS) (n = 10). Slices were examined by lightmicroscopy (LM) and scanning electron microscopy (SEM). For viability testing, specimens were connected to an isometric force transducer (2 x n = 9)...
November 1995: European Journal of Vascular and Endovascular Surgery
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