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https://www.readbyqxmd.com/read/29148926/identification-of-gene-candidates-associated-with-huanglongbing-tolerance-using-candidatus-liberibacter-asiaticus-flagellin-22-as-a-proxy-to-challenge-citrus
#1
Qingchun Shi, Vicente J Febres, Shujian Zhang, Fahong Yu, Greg McCollum, David G Hall, Gloria A Moore, Ed Stover
The 22-amino acid (flg22) pathogen-associated molecular pattern from the flagellin of Xanthomonas citri subsp. citri has been shown to induce defense responses correlated with citrus canker resistance. Here, flg22 of 'Candidatus Liberibacter asiaticus', the putative causal agent of Huanglongbing (HLB), elicited differential defense responses that were weaker than those from Xcc-flg22, between those of the HLB-tolerant mandarin cultivar Sun Chu Sha and susceptible grapefruit cultivar Duncan. Transcriptomics was used to compare the effect of CLas-flg22 and Xcc-flg22 between the citrus genotypes and identified 86 genes induced only by CLas-flg22 in the tolerant mandarin...
November 17, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29146805/the-structural-basis-of-flagellin-detection-by-naip5-a-strategy-to-limit-pathogen-immune-evasion
#2
Jeannette L Tenthorey, Nicole Haloupek, José Ramón López-Blanco, Patricia Grob, Elise Adamson, Ella Hartenian, Nicholas A Lind, Natasha M Bourgeois, Pablo Chacón, Eva Nogales, Russell E Vance
Robust innate immune detection of rapidly evolving pathogens is critical for host defense. Nucleotide-binding domain leucine-rich repeat (NLR) proteins function as cytosolic innate immune sensors in plants and animals. However, the structural basis for ligand-induced NLR activation has so far remained unknown. NAIP5 (NLR family, apoptosis inhibitory protein 5) binds the bacterial protein flagellin and assembles with NLRC4 to form a multiprotein complex called an inflammasome. Here we report the cryo-electron microscopy structure of the assembled ~1...
November 17, 2017: Science
https://www.readbyqxmd.com/read/29142160/immunogenicity-and-protective-efficacy-of-vibrio-vulnificus-flagellin-protein-flab-in-a-wound-infection-model
#3
Kohei Yamazaki, Takashige Kashimoto, Yuki Hashimoto, Takehiro Kado, Shunji Ueno
Vibrio vulnificus is known as an opportunistic bacterial pathogen that causes primary septicemia and wound infection in humans. Recently, the incidence of wound infection by V. vulnificus is increasing in warm countries. In this study, we examined a vaccine antigen against V. vulnificus in mice. FlaB, a component protein of the V. vulnificus flagellum, was expressed as a recombinant protein, named rFlaB. After immunization of mice with rFlaB, the mice were challenged by subcutaneous inoculation with V. vulnificus...
November 15, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29141156/plasmid-azobr_p1-borne-fabg-gene-for-putative-3-oxoacyl-acyl-carrier-protein-reductase-is-essential-for-proper-assembly-and-work-of-the-dual-flagellar-system-in-the-alphaproteobacterium-azospirillum-brasilense-sp245
#4
Yulia A Filip'echeva, Andrei V Shelud'ko, Alexei G Prilipov, Gennady L Burygin, Elizaveta M Telesheva, Stella S Yevstigneyeva, Marina P Chernyshova, Lilia P Petrova, Elena I Katsy
Azospirillum brasilense can swim and swarm owing to the activity of a constitutive polar flagellum (Fla) and inducible lateral flagella (Laf), respectively. Experimental data on the regulation of the Fla and Laf assembly in azospirilla are scarce. Here, the coding sequence (CDS) AZOBR_p1160043 (fabG1) for a putative 3-oxoacyl-[acyl-carrier protein (ACP)] reductase was found essential for the construction of both types of flagella. In an immotile leaky Fla- Laf- fabG1::Omegon-Km mutant, Sp245.1610, defects in flagellation and motility were fully complemented by expressing the CDS AZOBR_p1160043 from plasmid pRK415...
November 15, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29129586/mannose-binding-lectin-and-its-roles-in-immune-responses-in-grass-carp-ctenopharyngodon-idella-against-aeromonas-hydrophila
#5
Yunfei Dang, Xinzhan Meng, Shentong Wang, Lisen Li, Meng Zhang, Moyan Hu, Xiaoyan Xu, Yubang Shen, Liqun Lv, Rongquan Wang, Jiale Li
The complement system is a crucial component of the innate immune system that links innate and adaptive immunity via four pathways. Mannose-binding lectin (MBL), the initiating molecule of the lectin pathway, plays a significant role in the innate immune system in mammals and fish. Herein, we identified an MBL homolog (gcMBL) in grass carp (Ctenopharyngodon idella). The full-length 948 bp gcMBL cDNA includes a 741 bp open reading frame encoding a 246 amino acid protein with a signal peptide, collagen triple helix repeat domain, and a C-type lectin-like/link domain...
November 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29126991/rainbow-trout-oncorhynchus-mykiss-adipose-tissue-undergoes-major-changes-in-immune-gene-expression-following-bacterial-infection-or-stimulation-with-pro-inflammatory-molecules
#6
Kimberly A Veenstra, Eakapol Wangkahart, Tiehui Wang, Lincoln Tubbs, Juliette Ben Arous, Christopher J Secombes
In mammals, visceral adipose is increasingly seen as playing an important role in immune function with numerous pro-inflammatory, anti-inflammatory and immune-modulating proteins and peptides being identified in adipocytes. Adipose is also now known as a tissue that has an important role in the regulation of peritoneal immune responses. Despite this, only lately has consideration been given to visceral adipose as an important immune tissue in fish, especially in the context of intraperitoneal vaccination. The present study demonstrates that fish visceral adipose is capable of expressing a large range of immune molecules in response to stimulation with a live bacterium (A...
November 7, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29124898/impaired-competence-in-flagellar-mutants-of-bacillus-subtilis-is-connected-to-the-regulatory-network-governed-by-degu
#7
Theresa Hölscher, Tina Schiklang, Anna Dragoš, Anne-Kathrin Dietel, Christian Kost, Ákos T Kovács
The competent state is a developmentally distinct phase, in which bacteria are able to take up and integrate exogenous DNA into their genome. Bacillus subtilis is one of the naturally competent bacterial species and the domesticated laboratory strain 168 is easily transformable. In this study, we report a reduced transformation frequency of B. subtilis mutants lacking functional and structural flagellar components. This includes hag, the gene encoding the flagellin protein forming the filament of the flagellum...
November 10, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/29123526/toll-like-receptor-ligands-and-interferon-%C3%AE-synergize-for-induction-of-antitumor-m1-macrophages
#8
Elisabeth Müller, Panagiotis F Christopoulos, Sanjib Halder, Anna Lunde, Kahsai Beraki, Martin Speth, Inger Øynebråten, Alexandre Corthay
Tumor-associated macrophages may either promote or suppress tumor growth depending on their activation status. Interferon-γ (IFN-γ) has been identified as a key factor for inducing tumoricidal M1 phenotype in macrophages. However, it remains unclear whether IFN-γ is sufficient or if additional stimuli are required. Here, we tested IFN-γ and a panel of toll-like receptor (TLR) agonists for the ability to activate murine macrophages toward a tumoricidal M1 phenotype. The following TLR ligands were used: TLR1/TLR2 agonist Pam3CSK4, TLR2/TLR6 agonist lipotechoic acid, TLR3 agonist poly(I:C), TLR4 agonist lipopolysaccharide (LPS), TLR5 agonist flagellin, TLR7 agonist CL264, and TLR9 agonist CpG...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29123523/identification-of-an-evolutionarily-conserved-ankyrin-domain-containing-protein-caiap-which-regulates-inflammasome-dependent-resistance-to-bacterial-infection
#9
Sylwia D Tyrkalska, Sergio Candel, Ana B Pérez-Oliva, Ana Valera, Francisca Alcaraz-Pérez, Diana García-Moreno, María L Cayuela, Victoriano Mulero
Many proteins contain tandemly repeated modules of several amino acids, which act as the building blocks that form the underlying architecture of a specific protein-binding interface. Among these motifs and one of the most frequently observed is ankyrin repeats (ANK), which consist of 33 amino acid residues that are highly conserved. ANK domains span a wide range of functions, including protein-protein interactions, such as the recruitment of substrate to the catalytic domain of an enzyme, or the assembly of stable multiprotein complexes...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29111394/non-mammalian-toll-like-receptor-18-tlr18-recognizes-bacterial-pathogens-in-common-carp-cyprinus-carpio-l-indications-for-a-role-of-participation-in-the-nf-%C3%AE%C2%BAb-signaling-pathway
#10
Shijuan Shan, Dezhi Liu, Rongrong Liu, Yaoyao Zhu, Ting Li, Fumiao Zhang, Liguo An, Guiwen Yang, Hua Li
Toll-like receptors are important pattern recognition receptors that can recognize pathogen-associated molecular patterns (PAMPs) and play a critical role in innate immunity. In the present study, tlr18 was identified from common carp (Cyprinus carpio L.) (named Cctlr18). The deduced amino acid sequence contained only a signal peptide, eight LRR (leucine-rich repeat) motifs, a transmembrane region and a TIR (Toll/IL-1 receptor) domain. Phylogenetic analysis showed that CcTlr18 was most closely related to Ctenopharyngodon idella Tlr18...
October 27, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29109290/sea-anemone-model-has-a-single-toll-like-receptor-that-can-function-in-pathogen-detection-nf-%C3%AE%C2%BAb-signal-transduction-and-development
#11
Joseph J Brennan, Jonathan L Messerschmidt, Leah M Williams, Bryan J Matthews, Marinaliz Reynoso, Thomas D Gilmore
In organisms from insects to vertebrates, Toll-like receptors (TLRs) are primary pathogen detectors that activate downstream pathways, specifically those that direct expression of innate immune effector genes. TLRs also have roles in development in many species. The sea anemone Nematostella vectensis is a useful cnidarian model to study the origins of TLR signaling because its genome encodes a single TLR and homologs of many downstream signaling components, including the NF-κB pathway. We have characterized the single N...
November 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29097088/small-rna-big-effect-control-of-flagellin-production
#12
Aleksandra A Miranda-CasoLuengo, Stefani C Kary, Marc Erhardt, Carsten Kröger
Many bacteria move in their environment using a remarkable, rotating nanomachine - the flagellum. In a recent publication, Choi et al. report a new addition to the group of flagellar regulators, a trans-acting small RNA (sRNA).
October 30, 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/29091745/h-ns-represses-transcription-of-the-flagellin-gene-lafa-of-lateral-flagella-in-vibrio-parahaemolyticus
#13
Yan Wang, Zhang Yiquan, Zhe Yin, Jie Wang, Yongzhe Zhu, Haoran Peng, Dongsheng Zhou, Zhongtian Qi, Wenhui Yang
Swarming motility is ultimately mediated by the proton-powered lateral flagellar (laf) system in Vibrio parahaemolyticus. Expression of laf is tightly regulated by a number of environmental conditions and regulatory factors. The nucleoid-associated DNA-binding protein H-NS is a small and abundant protein that is widely distributed in bacteria, and H-NS like proteins-dependent expression of laf genes have been identified in V. cholerae and V. parahaemolyticus. The data presented here shows that H-NS acts as a repressor of the swarming motility in V...
November 1, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29080090/flagellin-glycosylation-with-pseudaminic-acid-in-campylobacter-and-helicobacter-prospects-for-development-of-novel-therapeutics
#14
REVIEW
Abu Iftiaf Md Salah Ud-Din, Anna Roujeinikova
Many pathogenic bacteria require flagella-mediated motility to colonise and persist in their hosts. Helicobacter pylori and Campylobacter jejuni are flagellated epsilonproteobacteria associated with several human pathologies, including gastritis, acute diarrhea, gastric carcinoma and neurological disorders. In both species, glycosylation of flagellin with an unusual sugar pseudaminic acid (Pse) plays a crucial role in the biosynthesis of functional flagella, and thereby in bacterial motility and pathogenesis...
October 27, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29076618/glycosylate-and-move-the-glycosyltransferase-maf-is-involved-in-bacterial-flagella-formation
#15
Gerlind Sulzenbacher, Véronique Roig-Zamboni, Régine Lebrun, Yann Guérardel, Dorothée Murat, Pascal Mansuelle, Nao Yamakawa, Xin-Xin Quian, Renaud Vincentelli, Yves Bourne, Long-Fei Wu, François Alberto
The flagella of various Gram-negative bacteria are decorated with diverse glycan structures, amongst them nonulosonic acids related to the sialic acid family. Although nonulosonic sugar biosynthesis pathways have been dissected in various pathogens, the enzymes transferring the sugars onto flagellin are still poorly characterized. The deletion of genes coding for Motility associated factors (Mafs) found in many pathogenic strains systematically gives rise to non-flagellated bacteria lacking specific nonulosonic sugars on the flagellins, therefore relating Maf function to flagellin glycosylation and bacterial motility...
October 27, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/29075278/hop-sti1-a-two-faced-cochaperone-involved-in-pattern-recognition-receptor-maturation-and-viral-infection
#16
Christian E Lamm, Max E Kraner, Jörg Hofmann, Frederik Börnke, Hans-Peter Mock, Uwe Sonnewald
Perception of pathogens by host pattern recognition receptors (PRRs) or R proteins is a prerequisite to promote successful immune responses. The Hsp70/Hsp90 organizing protein Hop/Sti1, a multifunctional cochaperone, has been implicated in the maturation of a receptor-like kinase (RLK) necessary for chitin sensing. However, it remains unknown whether Hop/Sti1 is generally participating in PRR genesis. Using RNA-interference (RNAi), we silenced Hop/Sti1 expression in Nicotiana tabacum to gain further insight into the role of the cochaperone in plant defense responses...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29062200/cloning-and-sequence-analysis-of-hyaluronoglucosaminidase-nagh-gene-of-clostridium-chauvoei
#17
Saroj K Dangi, Pavan Kumar Yadav, Aakanksha Tiwari, Viswas Konasagara Nagaleekar
AIM: Blackleg disease is caused by Clostridium chauvoei in ruminants. Although virulence factors such as C. chauvoei toxin A, sialidase, and flagellin are well characterized, hyaluronidases of C. chauvoei are not characterized. The present study was aimed at cloning and sequence analysis of hyaluronoglucosaminidase (nagH) gene of C. chauvoei. MATERIALS AND METHODS: C. chauvoei strain ATCC 10092 was grown in ATCC 2107 media and confirmed by polymerase chain reaction (PCR) using the primers specific for 16-23S rDNA spacer region...
September 2017: Veterinary World
https://www.readbyqxmd.com/read/29038601/a-structural-model-of-flagellar-filament-switching-across-multiple-bacterial-species
#18
Fengbin Wang, Andrew M Burrage, Sandra Postel, Reece E Clark, Albina Orlova, Eric J Sundberg, Daniel B Kearns, Edward H Egelman
The bacterial flagellar filament has long been studied to understand how a polymer composed of a single protein can switch between different supercoiled states with high cooperativity. Here we present near-atomic resolution cryo-EM structures for flagellar filaments from both Gram-positive Bacillus subtilis and Gram-negative Pseudomonas aeruginosa. Seven mutant flagellar filaments in B. subtilis and two in P. aeruginosa capture two different states of the filament. These reliable atomic models of both states reveal conserved molecular interactions in the interior of the filament among B...
October 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/29037578/a-phase-i-safety-and-immunogenicity-dose-escalation-trial-of-plague-vaccine-flagellin-f1-v-in-healthy-adult-volunteers-dmid-08-0066
#19
Sharon E Frey, Kathleen Lottenbach, Irene Graham, Edwin Anderson, Kanwaldeep Bajwa, Ryan C May, Steven B Mizel, Aaron Graff, Robert B Belshe
INTRODUCTION: Intentional aerosolization of Yersinia pestis may result in pneumonic plague which is highly fatal if not treated early. METHODS: We conducted a phase 1 randomized, double blind (within each group), placebo controlled, dose escalation trial to evaluate a plague vaccine, Flagellin/F1/V, in healthy adults aged 8 through 45years. Vaccine was administered intramuscularly on Days 0 and 28 at a dose of 1, 3, 6 or 10mcg. Subjects were observed for 4h after vaccination for cytokine release syndrome...
October 13, 2017: Vaccine
https://www.readbyqxmd.com/read/29018440/human-gut-symbiont-roseburia-hominis-promotes-and-regulates-innate-immunity
#20
Angela M Patterson, Imke E Mulder, Anthony J Travis, Annaig Lan, Nadine Cerf-Bensussan, Valerie Gaboriau-Routhiau, Karen Garden, Elizabeth Logan, Margaret I Delday, Alistair G P Coutts, Edouard Monnais, Vanessa C Ferraria, Ryo Inoue, George Grant, Rustam I Aminov
OBJECTIVE: Roseburia hominis is a flagellated gut anaerobic bacterium belonging to the Lachnospiraceae family within the Firmicutes phylum. A significant decrease of R. hominis colonization in the gut of ulcerative colitis patients has recently been demonstrated. In this work, we have investigated the mechanisms of R. hominis-host cross talk using both murine and in vitro models. DESIGN: The complete genome sequence of R. hominis A2-183 was determined. C3H/HeN germ-free mice were mono-colonized with R...
2017: Frontiers in Immunology
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