keyword
MENU ▼
Read by QxMD icon Read
search

Urease test

keyword
https://www.readbyqxmd.com/read/28934419/characterizing-antigenic-determinants-in-helicobacter-pylori-caga-capable-of-detecting-serum-antibodies-in-children
#1
Min-Kyoung Shin, Jin-Su Jun, Soon-Wook Kwon, Dong-Hae Lee, Jong-Hun Ha, Jin-Sik Park, Hyung Lyun Kang, Seung Chul Baik, Ji Sook Park, Ji-Hyun Seo, Hee-Shang Youn, Myung Je Cho, Woo Kon Lee
Helicobacter pylori can persistently colonize the mucosa of the human stomach, resulting in gastric disorders. Endoscopic biopsy for rapid urease test and histopathologic examination are considered as the most accurate diagnostic methods for H. pylori infection. Serological methods are recommended for children because of invasiveness of the diagnosis mentioned above. Here, the cytotoxin-associated gene A protein (Cag A), as an immunodominant antigen, was subdivided to determine which regions harbor antigenicity for humans...
November 30, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28934415/first-report-of-urease-activity-in-the-novel-systemic-fungal-pathogen-emergomyces-africanus-a-comparison-with-the-neurotrope-cryptococcus-neoformans
#2
Barbra Lerm, Chris Kenyon, Ilan S Schwartz, Heinrich Kroukamp, Riaan de Witt, Nelesh P Govender, G Sybren de Hoog, Alfred Botha
Cryptococcus neoformans is an opportunistic pathogen responsible for the AIDS-defining illness, cryptococcal meningitis. During the disease process, entry of cryptococcal cells into the brain is facilitated by virulence factors that include urease enzyme activity. A novel species of an Emmonsia-like fungus, recently named Emergomyces africanus, was identified as a cause of disseminated mycosis in HIV-infected persons in South Africa. However, in contrast to C. neoformans, the enzymes produced by this fungus, some of which may be involved in pathogenesis, have not been described...
November 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28927301/factors-for-improving-the-diagnostic-efficiency-of-the-rapid-urease-test-from-the-gastric-corpus
#3
Jun-Hyung Cho, Seong Ran Jeon, Hyun Gun Kim, So-Young Jin, Suyeon Park
OBJECTIVE: This study aimed to evaluate the optimal biopsy site for Helicobacter pylori detection by comparing the results of rapid urease test (RUT) between the gastric corpus and the antrum. METHODS: A biopsy specimen from each subject was obtained from the corpus and from the antrum. For each subject, the two specimens were separately immersed in two different RUT kits. Positive reaction times were measured at 20 minutes and 1, 3, and 24 hours. If either of the two RUT kits showed a positive reaction, H...
September 19, 2017: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/28912541/coprophagous-features-in-carnivorous-nepenthes-plants-a-task-for-ureases
#4
Ayufu Yilamujiang, Anting Zhu, Rodrigo Ligabue-Braun, Stefan Bartram, Claus-Peter Witte, Rainer Hedrich, Mitsuyasu Hasabe, Caroline R Schöner, Michael G Schöner, Gerald Kerth, Célia R Carlini, Axel Mithöfer
Most terrestrial carnivorous plants are specialized on insect prey digestion to obtain additional nutrients. Few species of the genus Nepenthes developed mutualistic relationships with mammals for nitrogen supplementation. Whether dietary changes require certain enzymatic composition to utilize new sources of nutrients has rarely been tested. Here, we investigated the role of urease for Nepenthes hemsleyana that gains nitrogen from the bat Kerivoula hardwickii while it roosts inside the pitchers. We hypothesized that N...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28860835/lymphoid-follicles-in-children-with-helicobacter-pylori-negative-gastritis
#5
Efrat Broide, Vered Richter, Sonia Mendlovic, Tzippora Shalem, Adi Eindor-Abarbanel, Steven F Moss, Haim Shirin
PURPOSE: The prevalence of Helicobacter pylori gastritis has been declining, whereas H. pylori-negative gastritis has become more common. We evaluated chronic gastritis in children with regard to H. pylori status and celiac disease (CD). PATIENTS AND METHODS: Demographic, clinical, endoscopic, and histologic features of children who underwent elective esophagogastroduodenoscopy were reviewed retrospectively. Gastric biopsies from the antrum and corpus of the stomach were graded using the Updated Sydney System...
2017: Clinical and Experimental Gastroenterology
https://www.readbyqxmd.com/read/28857733/mycobacterium-stephanolepidis-sp-nov-a-rapidly-growing-species-related-to-mycobacterium-chelonae-isolated-from-marine-teleost-fish-stephanolepis-cirrhifer
#6
Hanako Fukano, Shinpei Wada, Osamu Kurata, Kinya Katayama, Nagatoshi Fujiwara, Yoshihiko Hoshino
A previously undescribed rapidly growing, non-pigmented mycobacterium was identified based on biochemical and nucleic acid analyses, as well as growth characteristics. Seven isolates were cultured from samples collected from five thread-sail filefish (Stephanolepis cirrhifer) and two farmed black scraper (Thamnaconus modestus). Bacterial growth occurred at 15-35 °C on Middlebrook 7H11 agar. The bacteria were positive for catalase activity at 68 °C and urease activity, intermediate for iron uptake, and negative for Tween 80 hydrolysis, nitrate reduction, semi-quantitative catalase activity and arylsulfatase activity at day 3...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28844484/tlr2-arg677trp-but-not-tlr2-196-to-174-ins-del-and-arg753gln-polymorphism-alter-the-risk-of-peptic-ulcer-in-north-of-iran
#7
Maryam Habibzadeh, Mehdi Tourani, Javad Shokri-Shirvani, Amrollah Mostafazadeh, Soraya Khafri, Hamid Reza Nouri
BACKGROUND: Most polymorphisms that occur in TLR-2 are associated with gastrointestinal disorders such as peptic ulcer disease (PUD). Hence, in current study, association between TLR2-196 to -174 ins/del, Arg753Gln and Arg677Trp polymorphisms and risk of PUD development in north of Iran was evaluated. METHODS: This case-control study included 50 patients with PUD as cases and 50 people without peptic ulcer as control group. Blood and endoscopic biopsies were collected...
August 22, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/28839492/misinterpretation-of-gram-stain-from-the-stationary-growth-phase-of-positive-blood-cultures-for-brucella-and-acinetobacter-species
#8
Ali M Bazzi, Jaffar A Al-Tawfiq, Ali A Rabaan
INTRODUCTION: Acinetobacter baumannii and Brucella species are Gram-negative organisms that are vulnerable to misinterpretation as Gram-positive or Gram-variable in blood cultures. OBJECTIVE: We assess the random errors in gram stain interpretation to reduce the likelihood of such errors and therefore patient harm. METHODOLOGY: Aerobic and anaerobic blood cultures from two patients in an acute care facility in Saudi Arabia were subjected to preliminary Gram-staining...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28814259/may-gender-or-ethnicity-affect-delta-over-baseline-values-obtained-by-13-c-urea-breath-test
#9
Carmine Petruzziello, Dario Sinatti, Maria Gnarra, Alessio Migneco, Valentina Tesori, Cristina Graziani, Antonio Gasbarrini, Francesco Franceschi, Veronica Ojetti
INTRODUCTION: 13C-urea breath test (UBT) is a non-invasive test for detecting active H.pylori infection. Previous studies showed a correlation of delta over baseline (DOB) values with bacterial load, mucosal inflammation and successful eradication. Gender has been shown to affect DOB in children. Aim of our study was to verify whether gender or ethnicity affects DOB in adults. PATIENTS AND METHODS: We retrospectively analyzed data of 2922 patients (1024M/1898F mean age 47±15 years) that underwent UBT in our outpatient unit, from October 2015 to October 2016...
August 16, 2017: Reviews on Recent Clinical Trials
https://www.readbyqxmd.com/read/28811799/diversity-physiochemical-and-phylogenetic-analyses-of-bacteria-isolated-from-various-drinking-water-sources
#10
Neveen H Eid, Huda A Al Doghaither, Taha A Kumosani, Munazza Gull
OBJECTIVE: To evaluate the indigenous bacterial strains of drinking water from the most commercial water types including bottled and filtered water that are currently used in Saudi Arabia. METHODS: Thirty randomly selected commercial brands of bottled water were purchased from Saudi local markets. Moreover, samples from tap water and filtered water were collected in sterilized glass bottles and stored at 4°C. Biochemical analyses including pH, temperature, lactose fermentation test (LAC), indole test (IND), methyl red test (MR), Voges-Proskauer test (VP), urease test (URE), catalase test (CAT), aerobic and anaerobic test (Ae/An) were measured...
May 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28809946/structure-function-analyses-of-metal-binding-sites-of-hypa-reveal-residues-important-for-hydrogenase-maturation-in-helicobacter-pylori
#11
Faith C Blum, Heidi Q Hu, Stephanie L Servetas, Stéphane L Benoit, Robert J Maier, Michael J Maroney, D Scott Merrell
The nickel-containing enzymes of Helicobacter pylori, urease and hydrogenase, are essential for efficient colonization in the human stomach. The insertion of nickel into urease and hydrogenase is mediated by the accessory protein HypA. HypA contains an N-terminal nickel-binding site and a dynamic structural zinc-binding site. The coordination of nickel and zinc within HypA is known to be critical for urease maturation and activity. Herein, we test the hydrogenase activity of a panel of H. pylori mutant strains containing point mutations within the nickel- and zinc-binding sites...
2017: PloS One
https://www.readbyqxmd.com/read/28794636/helicobacter-pylori-infection-and-severity-of-coronary-atherosclerosis-in-patients-with-chronic-coronary-artery-disease
#12
Anita Jukic, Dorotea Bozic, Dusko Kardum, Tina Becic, Bruno Luksic, Mislav Vrsalovic, Marko Ljubkovic, Damir Fabijanic
AIM: Controversy exists concerning the relation between Helicobacter pylori (HP) infection and coronary artery disease (CAD). We aimed to examine the relationship between HP infection and severity of coronary atherosclerosis in patients with chronic CAD. PATIENTS AND METHODS: A total of 150 patients (109 [73%] men; mean age 62.61±10.23 years) scheduled for coronary artery bypass grafting surgery were consecutively enrolled in the cross-sectional study. According to rapid urease test and/or gastric biopsy samples stained with hematoxylin and eosin and according to Giemsa, patients were classified as HP positive (n=87; 58%) or HP negative (n=63; 42%)...
2017: Therapeutics and Clinical Risk Management
https://www.readbyqxmd.com/read/28791813/an-evaluation-of-selected-oral-health-indicators-and-cariogenic-bacteria-titer-in-patients-with-helicobacter-pylori
#13
Jakub Urban, Rafał Koszowski, Anna Płachetka, Andrzej Wiczkowski
BACKGROUND: Studies based on polymerase chain reaction (PCR) techniques indicate that Helicobacter pylori can be constantly or temporarily present in the oral cavity in virulent or non-virulent form. Streptococcus mutans exerts a strong inhibitory effect on H. pylori. OBJECTIVES: The aim of the present study was to investigate the prevalence and virulence of H. pylori in the oral cavity and the correlation of these factors with oral health and cariogenic bacteria titer...
May 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/28791039/impact-of-transgenic-brassica-napus-harboring-the-antifungal-synthetic-chitinase-nic-gene-on-rhizosphere-microbial-diversity-and-enzyme-activities
#14
Mohammad S Khan, Syed U Sadat, Asad Jan, Iqbal Munir
Transgenic Brassica napus harboring the synthetic chitinase (NiC) gene exhibits broad-spectrum antifungal resistance. As the rhizosphere microorganisms play an important role in element cycling and nutrient transformation, therefore, biosafety assessment of NiC containing transgenic plants on soil ecosystem is a regulatory requirement. The current study is designed to evaluate the impact of NiC gene on the rhizosphere enzyme activities and microbial community structure. The transgenic lines with the synthetic chitinase gene (NiC) showed resistance to Alternaria brassicicola, a common disease causing fungal pathogen...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28764169/histomorphological-spectrum-of-duodenal-pathology-in-functional-dyspepsia-patients
#15
Anita Aujenath Chaudhari, Sharada Raju Rane, Meenal Vitthal Jadhav
INTRODUCTION: Functional Dyspepsia (FD) is one of the most common causes of gastrointestinal symptoms aetiology of which is poorly understood. AIM: To study duodenal histomorphological features and their relationship with Helicobacter pylori (H Pylori) infection in patients of FD. MATERIALS AND METHODS: This case control study included 50 cases of FD patients selected according to Rome III criteria and 30 age and sex matched controls. These were subjected to oesophago-gastro-duodenoscopy, rapid urease test for detection of H...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28745795/research-on-the-mechanism-of-hp-mediated-pi3k-akt-gsk3%C3%AE-pathways-in-gastric-cancer
#16
W Geng, H-Y Zhang
OBJECTIVE: This study aimed to investigate the mechanism of Helicobacter pylori (HP) mediated PI3K/AKT/GSK3β signal pathways in the occurrence of gastric cancer. PATIENTS AND METHODS: For this purpose, a total of 90 patients were selected; 30 were suffering from gastric cancer and gastric ulcer (observation group I), 30 were suffering from pure gastric ulcer (observation group II) and 30 were with chronic gastritis (control group), and the lesion tissues were extracted by using endoscopy...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28725170/helicobacter-pylori-infection-status-had-no-influence-on-upper-gastrointestinal-symptoms-a-cross-sectional-analysis-of-3-005-japanese-subjects-without-upper-gastrointestinal-lesions-undergoing-medical-health-checkups
#17
Tomomi Yoshioka, Eri Takeshita, Yasuhisa Sakata, Megumi Hara, Kayo Akutagawa, Natsuko Sakata, Hiroyoshi Endo, Takashi Ohyama, Keiji Matsunaga, Yuichiro Tanaka, Shinpei Shirai, Yoichiro Ito, Nanae Tsuruoka, Ryuichi Iwakiri, Motoyasu Kusano, Kazuma Fujimoto
BACKGROUND: This study aimed to evaluate the influence of Helicobacter pylori infection and its eradication on the upper gastrointestinal symptoms of relatively healthy Japanese subjects. METHODS: A total of 3,005 subjects (male/female: 1,549/1,456) undergoing medical health checkups were enrolled in the present study, at five hospitals in Saga, Japan, from January to December 2013. They had no significant findings following upper gastrointestinal endoscopy. All subjects completed a questionnaire that addressed a frequency scale for symptoms of gastroesophageal reflux disease...
2017: Esophagus: Official Journal of the Japan Esophageal Society
https://www.readbyqxmd.com/read/28702150/adding-ofloxacin-to-standard-triple-drug-regimens-increased-the-helicobacter-pylori-eradication-rate-data-from-randomized-clinical-trial
#18
Mahmoud Sadeghi-Hadad-Zavareh, Seyyedsaeid Mohammadi, Mahmoud Hajiahmadi, Mostafa Javanian, Masoomeh Habibian
BACKGROUND: The rate of Helicobacter pylori (H.pylori) eradication in dyspeptic patients using bismuth- based triple therapy is low due to bacterial that are resistant to antibiotics. The results of recent studies regarding levofloxacin have been encouraging, but the high cost of treatment prevents its routine administration. We, therefore, performed the present double-blind clinical trial to compare the efficiency of quadruple-drug regimen containing ofloxacin, clarithromycin, amoxicillin, and omeprazole and the same standard triple-therapy regimen minus ofloxacin in H...
2017: Caspian Journal of Internal Medicine
https://www.readbyqxmd.com/read/28667775/a-one-step-immune-chromatographic-helicobacter-pylori-stool-antigen-test-for-children-was-quick-consistent-reliable-and-specific
#19
Nicolas Kalach, Pierre Gosset, Eric Dehecq, Anne Decoster, Anne-France Georgel, Claire Spyckerelle, Stephanos Papadopoulos, Christophe Dupont, Josette Raymond
AIM: This French study assessed a quick, non-invasive, immuno-chromatographic, Helicobacter pylori (H. pylori) stool antigen test for detecting infections in children. METHODS: We enrolled 158 children, with a median age of 8.5 years (range eight months to 17 years), with digestive symptoms suggesting upper gastrointestinal tract disease. Upper digestive endoscopy was performed with gastric biopsy-specimens for histology, a rapid urease test, culture test and quantitative real-time polymerase chain reaction...
July 1, 2017: Acta Paediatrica
https://www.readbyqxmd.com/read/28647123/zinc-solubilizing-bacillus-spp-potential-candidates-for-biofortification-in-maize
#20
Muhammad Zahid Mumtaz, Maqshoof Ahmad, Moazzam Jamil, Tanveer Hussain
Bioaugmentation of Zn solubilizing rhizobacteria could be a sustainable intervention to increase bioavailability of Zn in soil which can be helpful in mitigation of yield loss and malnutrition of zinc. In present study, a number of pure rhizobacterial colonies were isolated from maize rhizosphere and screened for their ability to solubilize zinc oxide. These isolates were screened on the basis of zinc and phosphate solubilization, IAA production, protease production, catalase activity and starch hydrolysis...
September 2017: Microbiological Research
keyword
keyword
34024
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"