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Urease test

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https://www.readbyqxmd.com/read/28641404/ph-rather-than-nitrification-and-urease-inhibitors-determines-the-community-of-ammonia-oxidizers-in-a-vegetable-soil
#1
Ruijiao Xi, Xi-En Long, Sha Huang, Huaiying Yao
Nitrification inhibitors and urease inhibitors, such as nitrapyrin and N-(n-butyl) thiophosphoric triamide (NBPT), can improve the efficiencies of nitrogen fertilizers in cropland. However, their effects on ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) across different soil pH levels are still unclear. In the present work, vegetable soils at four pH levels were tested to determine the impacts of nitrification and urease inhibitors on the nitrification activities, abundances and diversities of ammonia oxidizers at different pHs by real-time PCR, terminal restriction fragment length polymorphism (T-RFLP) and clone sequence analysis...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28623137/azomethines-isoxazole-n-substituted-pyrazoles-and-pyrimidine-containing-curcumin-derivatives-urease-inhibition-and-molecular-modeling-studies
#2
Mahmood Ahmed, Muhammad Abdul Qadir, Abdul Hameed, Muhammad Nadeem Arshad, Abdullah M Asiri, Muhammad Muddassar
Curcumin has shown large number of pharmacological properties against different phenotypes of various disease models. Different synthetic routes have been employed to develop its various derivatives for diverse biological functions. In this study, curcumin derived azomethine, isoxazole, pyrimidines and N-substituted pyrazoles were synthesized to investigate their urease enzyme inhibition. The structures of newly synthesized compounds were described by IR, MS, (1)H NMR and (13)C NMR spectral data. Urease enzyme inhibition was evaluated through in vitro assays in which compound 8b was found to be the most potent (IC50 = 2...
June 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28615866/virulence-assay-and-role-of-bacillus-thuringiensis-ts110-as-biocontrol-agent-against-the-larval-stages-of-rice-leaffolder-cnaphalocrocis-medinalis
#3
Tuhin Subhra Ghosh, Soumendranath Chatterjee, Syed Afrin Azmi, Abhijit Mazumdar, Tushar Kanti Dangar
The bacterial isolate Bacillus thuringiensis TS110 was isolated from the rice field soil of Burdwan district, West Bengal, India. Bioassay test of the bacteria TS110 against 3rd, 4th and 5th instar larvae of Cnaphalocrocis medinalis was carried out. Cut leaf assay, potted plant assay and field assay were done. During filed assay, it has been observed that the LC50 (×10(7)) values of TS110 against 3rd, 4th and 5th instar larvae of C. medinalis were 3.77, 5.29, 4.83 and 4.93, 4.42, 4.72 in dry and wet season, respectively...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28601610/therapeutic-success-with-bismuth-containing-sequential-and-quadruple-regimens-in-helicobacter-pylori-eradication
#4
Oguzhan Ozturk, Levent Doganay, Yasar Colak, Feruze Yilmaz Enc, Celal Ulasoglu, Kamil Ozdil, Ilyas Tuncer
BACKGROUND AND STUDY AIMS: The success rate of Helicobacter pylori (H. pylori) eradication with the classical triple therapy is gradually declining. In this study, we aimed to compare and assess the efficacies of six different eradication regimens including sequential protocols. PATIENTS AND METHODS: Endoscopically confirmed nonulcer dyspepsia patients were enrolled. H. pylori presence was determined either histologically or by a rapid urease test. Treatment-naive patients were randomly assigned to an either one of three 10-day (OAC, OTMB, and OACB) or one of three sequential protocols (OA+OCM, OA+OCMB, and OA+OMDB) (O=omeprazole, A=amoxicillin, C=clarithromycin, T=tetracycline, M=metronidazole, B=bismuth, D=doxycycline)...
June 7, 2017: Arab Journal of Gastroenterology: the Official Publication of the Pan-Arab Association of Gastroenterology
https://www.readbyqxmd.com/read/28596600/detection-of-dna-amplicons-of-polymerase-chain-reaction-using-litmus-test
#5
Dingran Chang, Kha Tram, Ben Li, Qian Feng, Zhifa Shen, Christine H Lee, Bruno J Salena, Yingfu Li
We report on a new colorimetric DNA detection method that takes advantage of the power of polymerase chain reaction (PCR) and the simplicity of the classic litmus test. The strategy makes use of a modified set of primers for PCR to facilitate ensuing manipulations of resultant DNA amplicons: their tagging with urease and immobilization onto magnetic beads. The amplicon/urease-laden beads are then used to hydrolyze urea, resulting in the increase of pH that can be conveniently reported by a pH-sensitive dye...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28581271/helicobacter-pylori-antigens-inducing-early-immune-response-in-infants
#6
Ji Hyun Seo, Jong Hyuk Youn, Eun A Kim, Jin Su Jun, Ji Sook Park, Jung Sook Yeom, Jae Young Lim, Hyang Ok Woo, Hee Shang Youn, Gyung Hyuck Ko, Jin Sik Park, Seung Chul Baik, Woo Kon Lee, Myung Je Cho, Kwang Ho Rhee
To identify the Helicobacter pylori antigens operating during early infection in sera from infected infants using proteomics and immunoblot analysis. Two-dimensional (2D) large and small gel electrophoresis was performed using H. pylori strain 51. We performed 2D immunoglobulin G (IgG), immunoglobulin A (IgA), and immunoglobulin M (IgM) antibody immunoblotting using small gels on sera collected at the Gyeongsang National University Hospital from 4-11-month-old infants confirmed with H. pylori infection by pre-embedding immunoelectron microscopy...
July 2017: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/28575047/utility-of-normalized-genome-quantification-of-helicobacter-pylori-in-gastric-mucosa-using-an-in-house-real-time-polymerase-chain-reaction
#7
Ana Morilla, Santiago Melón, Marta E Álvarez-Argüelles, Edisa Armesto, Henar Villar, María de Oña
Traditional diagnostic assays for Helicobacter pylori detection have their limitations. Molecular methods can improve both diagnosis and understanding of gastric diseases. Here we describe an in-house quantitative real-time polymerase chain reaction (q-rt-PCR) for the detection of H. pylori in gastric biopsies which has been developed and has a detection limit of 10 copies, the specificity of which was tested against other gastric colonizer bacteria. In this study, 199 gastric biopsies from adults with different clinical gastric symptoms were examined...
2017: PloS One
https://www.readbyqxmd.com/read/28571727/efficacy-of-mentha-pulegium-extract-in-the-treatment-of-functional-dyspepsia-a-randomized-double-blind-placebo-controlled-clinical-trial
#8
Ahmad Khonche, Hasan Fallah Huseini, Hamed Abdi, Reza Mohtashami, Farzaneh Nabati, Saeed Kianbakht
ETHNOPHARMACOLOGICAL EVIDENCE: Mentha pulegium L. leaves are used in the Iranian traditional medicine for the treatment of functional dyspepsia. AIM OF STUDY: To study the efficacy and safety of M. pulegium in the treatment of functional dyspepsia patients fulfilling the Rome III criteria. MATERIALS AND METHODS: The efficacy and safety of a standardized Mentha pulegium leaf extract (drug extract ratio: 15.9:1, extraction solvent: 70% v/v aqueous ethanol) (330mg three times daily taken for 2 months) as add-on to one famotidine 40mg tablet per day in the treatment of 50 functional dyspepsia patients were compared with those of a parallel placebo group (n =50)...
May 30, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28571523/association-of-heterogenicity-of-helicobacter-pylori-cag-pathogenicity-island-with-peptic-ulcer-diseases-and-gastric-cancer
#9
J Khatoon, K N Prasad, R Prakash Rai, U C Ghoshal, N Krishnani
OBJECTIVE: To investigate the frequency and integrity of certain cag pathogenicity island genes (cagPAI) in Helicobacter pylori strains and their association with peptic ulcer disease (PUD) and gastric cancer. MATERIAL AND METHODS: We enrolled 240 adult patients [120 with functional dyspepsia (FD), 50 with PUD and 70 with gastric cancer] undergoing upper gastrointestinal endoscopy. H. pylori infection was diagnosed when either culture or any two of the three tests (rapid urease test, histopathology and specific ureA PCR) were positive...
June 2, 2017: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/28545189/outcomes-of-a-randomized-controlled-trial-comparing-modified-high-dose-omeprazole-and-amoxicillin-triple-therapy-with-standard-triple-therapy-for-helicobacter-pylori-eradication
#10
Kitti Chunlertlith, Uaepong Limpapanasit, Pisaln Mairiang, Suda Vannaprasaht, Wichittra Tassaneeyakul, Apichat Sangchan, Kookwan Sawadpanich, Tanita Suttichaimongkol, Jamrus Pongpit, Moragot Pattarapongsin
Background: Helicobacter pylori (H. pylori) infection is related to peptic ulcer diseases and gastric cancer and eradication of H. pylori should be expected to decrease the risk of their development. Factors affecting H. pylori eradication are antibiotic resistance, CYP2C19 genotypes, drug regimen and patient compliance. Increment of omeprazole and amoxicillin dosage in clarithromycin-containing triple therapy regimen may overcome these problems and may be a better choice than the conventional clarithromycin-containing triple therapy regimen...
April 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28535789/a-multi-target-therapeutic-potential-of-prunus-domestica-gum-stabilized-nanoparticles-exhibited-prospective-anticancer-antibacterial-urease-inhibition-anti-inflammatory-and-analgesic-properties
#11
Nazar Ul Islam, Raza Amin, Muhammad Shahid, Muhammad Amin, Sumera Zaib, Jamshed Iqbal
BACKGROUND: Phytotherapeutics exhibit diverse pharmacological effects that are based on the combined action of a mixture of phytoconstituents. In this study, Prunus domestica gum-loaded, stabilized gold and silver nanoparticles (Au/Ag-NPs) were evaluated for their prospective anticancer, antibacterial, urease-inhibition, anti-inflammatory, and analgesic properties. METHODS: Au/Ag-NPs were biosynthesized and characterized with UV-Vis, FTIR, SEM, EDX, and XRD techniques...
May 23, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28531911/risk-factors-for-gastric-tumorigenesis-in-underlying-gastric-mucosal-atrophy
#12
Ji Hyun Song, Sang Gyun Kim, Eun Hyo Jin, Joo Hyun Lim, Sun Young Yang
Background/Aims: Atrophic gastritis is considered a premalignant lesion. We aimed to evaluate the risk factors for gastric tumorigenesis in underlying mucosal atrophy. Methods: A total of 10,185 subjects who underwent upper gastrointestinal endoscopy between 2003 and 2004 were enrolled in this retrospective cohort study. Follow-up endoscopy was performed between 2005 and 2014. Atrophic gastritis and intestinal metaplasia were assessed by endoscopy using the Kimura-Takemoto classification...
May 23, 2017: Gut and Liver
https://www.readbyqxmd.com/read/28531095/synthesis-of-1-2-3-triazolo-4-5-h-quinolone-derivatives-with-novel-anti-microbial-properties-against-metronidazole-resistant-helicobacter-pylori
#13
Mohammad Abu-Sini, Amal Mayyas, Nehaya Al-Karablieh, Rula Darwish, Yusuf Al-Hiari, Talal Aburjai, Shereen Arabiyat, Luay Abu-Qatouseh
Helicobacter pylori infection can lead to gastritis, peptic ulcer, and the development of mucosa associated lymphoid tissue (MALT) lymphoma. Treatment and eradication of H. pylori infection can prevent relapse and accelerate the healing of gastric and duodenal ulcers as well as regression of malignancy. Due to the increasing emergence of antibiotic resistance among clinical isolates of H. pylori, alternative approaches using newly discovered antimicrobial agents in combination with the standard antibiotic regimens for the treatment of H...
May 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28515219/a-novel-stool-pcr-test-for-helicobacter-pylori-may-predict-clarithromycin-resistance-and-patient-eradication-at-a-high-rate
#14
Erin Beckman, Ilaria Saracino, Giulia Fiorini, Courtney Clark, Vladimir Slepnev, Denise Patel, Clarissa Gomez, Reddy Ponaka, Vecheslav Elagin, Dino Vaira
Objectives: Clarithromycin-based regimens are commonly used as a first line therapy in H. pylori positive patients, however treatment failures are associated with resistance to clarithromycin. The aim of this study was to evaluate the feasibility of using stool samples to detect the presence of H. pylori DNA, while concurrently detecting mutations associated with the resistance to clarithromycin.Methods: Total DNA was extracted from 294 raw stools of H pylori positive and negative patients. TaqMan real-time PCR amplification was used to detect the presence of H...
May 17, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28507617/serum-vegf-levels-in-helicobacter-pylori-infection-and-correlation-with-helicobacter-pylori-caga-and-vaca-genes
#15
Gontar Siregar, Dina Sari, Taufik Sungkar
BACKGROUND: Helicobacter pylori vacA and cagA genes are associated with higher virulence. Vascular Endothelial Growth Factor (VEGF) is one important marker for neo-angiogenesis. AIM: The purpose of this study was to investigate the relationship between VEGF serum levels with cagA and vacA genes in H. pylori infection. METHODS: A cross-sectional study was done on eighty patients that consecutive admitted to endoscopy unit. The diagnosis of H...
April 15, 2017: Open Access Macedonian Journal of Medical Sciences
https://www.readbyqxmd.com/read/28502132/evaluation-of-non-invasive-versus-invasive-methods-for-diagnosis-of-helicobacter-pylori-infection-among-patients-with-gastroduodenal-disorders
#16
Mona A Hassan, Entsar H Ahmed, Bahaa-Eldin A Abdel-Raady, Ibrahim A Amin, Abeer ElRefaiy Refaiy, Gamal M Soliman
H. pylori, a spiral gram-negative bacterium, is associated with gastroduodenal diseases. All H. pylori diagnostic assays have limitations. Cytotoxin-associated gene A (cag A), a virulence marker, can be identified by PCR. We evaluated H. pylori diagnostic methods, invasive: rapid urease test (RUT), and histopathological examination (HE), and serology as non-invasive method. Positive cases were studied for presence of cag A gene. Upper endoscopies and gastric biopsies were performed on 67 dyspeptic patients for RUT, HE and PCR...
June 2016: Egyptian Journal of Immunology
https://www.readbyqxmd.com/read/28489837/-validation-of-the-rapid-urease-test-for-the-detection-of-helicobacter-pylori-in-a-peruvian-hospital
#17
Vladimir Espinoza Ildefonso, Harold Tabori Peinado, Claudia Meza Borja, Alejandra Bussalleu Cavero, Luis Vásquez Elera, Víctor Aguilar Sánchez, José Luis Pinto Valdivia, Jaime Cok, Alejandro Bussalleu Rivera
OBJECTIVE: To validate a rapid urease test (RUT) in Cayetano Heredia Hospital (HCH) in Lima, Peru. MATERIALS AND METHODS: This is a prospective observational study that included 181 patients over 18 years old with dyspeptic symptoms. All of them underwent upper gastrointestinal endoscopy at the Department of Gastroenterology at HCH. They had not received, during the last four weeks, proton pump inhibitors (PPIs), bismuth or antibiotics. Two biopsies of antrum were taken, one to perform the TRU (Sensibacter pylori test®) and the other one for pathology, in order to determine by both methods the presence of H...
January 2017: Revista de Gastroenterología del Perú: órgano Oficial de la Sociedad de Gastroenterología del Perú
https://www.readbyqxmd.com/read/28483407/evaluation-of-diagnostic-accuracy-of-two-rapid-stool-antigen-tests-using-an-immunochromatographic-assay-to-detect-helicobacter-pylori
#18
Joyce Matie Kinoshita da Silva-Etto, Rejane Mattar, Cibele Aparecida Villares-Lopes, Sergio Barbosa Marques, Flair José Carrilho
OBJECTIVES: The stool antigen assay for H. pylori infection diagnosis with monoclonal antibodies is a simple and recommended technique by the Maastricht V/Florence consensus report. Recently, Pylori K-Set K-1219 (Coris Bioconcept Sprl, Belgium) and HP-F23 (Symbiosys, Brazil) have been made commercially available in Brazil. Thus, the aim of this study was to evaluate the diagnostic accuracies of these two rapid stool antigen tests by immunochromatographic assays (index tests) for the clinical practice...
May 5, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28465061/construction-of-ferrocene-modified-conducting-polymer-based-amperometric-urea-biosensor
#19
Muamer Dervisevic, Esma Dervisevic, Mehmet Senel, Emre Cevik, Huseyin Bekir Yildiz, Pınar Camurlu
Herein, an electrochemical urea sensing bio-electrode is reported that has been constructed by firstly electropolymerizing 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline monomer (SNS-Aniline) on Pencil Graphite Electrode (PGE), then modifying the polymer coated electrode surface with di-amino-Ferrocene (DAFc) as the mediator, and lastly Urease enzyme through glutaraldehyde crosslinking. The effect of pH, temperature, polymer thickness, and applied potential on the electrode current response was investigated besides performing storage and operational stability experiments with the interference studies...
July 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28464776/gastric-mucosal-oxidative-stress-markers-in-children-with-helicobacter-pylori-infection
#20
Adel Abd Elhaleim Hagag, Saleh M Amin, Mohammed H Emara, Sally E Abo-Resha
BACKGROUND: 'Helicobacter pylori' "H. pylori" is one of the most common infections that colonizes human gastric mucosa and generates reactive oxygen and nitrogen species. The aim of this study was to evaluate the oxidative stress markers in the gastric mucosa of "H. pylori"- infected children. PATIENTS AND METHODS: The present study was carried out on 60 children infected with "H. pylori" including 28 males, 32 females with their age ranging from 7-10 years and mean age value of 8...
May 2, 2017: Infectious Disorders Drug Targets
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