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Urease test

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https://www.readbyqxmd.com/read/28215808/urea-stabilisation-and-concentration-for-urine-diverting-dry-toilets-urine-dehydration-in-ash
#1
Jenna Senecal, Björn Vinnerås
Human excreta contain the same nitrogen, phosphorus and potassium (N-P-K) as the fertilisers used to produce the food consumed. However, human excreta are considered unwanted waste throughout the world, creating humanitarian and environmental problems. In order to replace the nutrients removed from fields during crop harvesting, more fertilisers are manufactured, in processes contributing to environmental changes at global level. The limitation of human urine as a fertiliser is its low nutrient concentration compared with commercial fertilisers...
February 16, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28215482/enzymatic-characterization-of-clinical-and-environmental-cryptococcus-neoformans-strains-isolated-in-italy
#2
Gabriella Pini, Elisabetta Faggi, Enza Campisi
BACKGROUND: Cryptococcus neoformans is an encapsulated yeast causing mainly opportunistic infections. The virulence factors involved in cryptococcosis pathogenesis include the presence and the size of the polysaccharide capsule, the production of melanin by phenoloxidase, the growth at 37°C and the enzyme secretion like proteinase, phospholipase and urease. Many other enzymes are secreted by C. neoformans but their role in the fungus virulence is not yet known. AIMS: In order to investigate this topic, we compared the phospholipase production between strains from patients and from bird droppings, and we examined its relationship to phenoloxidase production...
February 16, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28207990/carbonic-anhydrase-and-urease-inhibitory-potential-of-various-plant-phenolics-using-in-vitro-and-in-silico-methods
#3
Abdur Rauf, Muslim Raza, Muhammad Saleem, Ufuk Ozgen, Esen Sezen Karaoglan, Gulin Renda, Erhan Palaska, Ilkay Erdogan Orhan
Plant phenolics are known to display many pharmacological activities. In the current study, eight phenolic compounds [e.g. luteolin 5-O-β-glucoside (1), methyl rosmarinate (2), apigenin (3), vicenin 2 (4), lithospermic acid (5), soyasaponin II (6), rubiadin 3-O-β-primeveroside (7), 4(3,4dihydroxybenzoyloxymethyl)phenyl-O-β-D-glucopyranoside (8)] isolated from various plant species were tested at 0.2 mM against carbonic anhydrase-II (CA-II) and urease using microtiter assays. Urease inhibition rate for compounds 1-8 ranged between 5...
February 16, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28193167/helicobacter-pylori-prevalence-and-clinical-significance-in-patients-with-quiescent-crohn-s-disease
#4
Adi Lahat, Uri Kopylov, Sandra Neuman, Nina Levhar, Doron Yablecovitch, Benjamin Avidan, Batia Weiss, Shomron Ben-Horin, Rami Eliakim
BACKGROUND: Helicobacter pylori (HP) infection is present in about 50% of the global population, and is associated with chronic gastritis, peptic disease and gastric malignancies. HP prevalence in Crohn's disease (CD) patients was shown to be low compared to the general population, and its influence on disease activity is yet to be determined. Our aims were to determine the prevalence of HP in a selected group of CD patients with quiescent disease, and to assess the influence of its eradication on disease activity and endoscopic and laboratory activity measures...
February 13, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/28191542/loose-ends-in-the-eradication-of-helicobacter-pylori-infection
#5
Armando Peixoto, Marco Silva, Rui Gaspar, Rui Morais, Rosa Ramalho, Guilherme Macedo, João Santos-Antunes
The eradication of Helicobacter pylori is essential for prevention and treatment of various conditions associated with this infection. However, its effectiveness is limited and influenced by factors linked to the bacteria and the host. In particular, influence of the biotype, smoking, diabetes mellitus, and previous treatment failure in eradication is understudied. Our center proposed to evaluate these aspects in a real life cohort by applying a questionnaire with demographic and lifestyle variables in patients who consecutively underwent urease breath test after the eradication therapy...
December 1, 2016: Journal of Translational Internal Medicine
https://www.readbyqxmd.com/read/28179897/comparative-transcriptomics-reveals-discrete-survival-responses-of-s-aureus-and-s-epidermidis-to-sapienic-acid
#6
Josephine C Moran, Jamal A Alorabi, Malcolm J Horsburgh
Staphylococcal colonization of human skin is ubiquitous, with particular species more frequent at different body sites. Whereas Staphylococcus epidermidis can be isolated from the skin of every individual tested, Staphylococcus aureus is isolated from <5% of healthy individuals. The factors that drive staphylococcal speciation and niche selection on skin are incompletely defined. Here we show that S. aureus is inhibited to a greater extent than S. epidermidis by the sebaceous lipid sapienic acid, supporting a role for this skin antimicrobial in selection of skin staphylococci...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28149841/effects-of-curcumin-on-helicobacter-pylori-infection
#7
Vaclav Vetvicka, Jana Vetvickova, Rafael Fernandez-Botran
BACKGROUND: Curcumin is a well-established natural molecule with significant biological and pharmaceutical effects. Its effects on Helicobacter pylori (H. pylori) infection have been repeatedly confirmed both in animal and human models. This study directly compared five different samples to evaluate if the effects are general or if they differ among samples. METHODS: Using a mouse model, we studied the effects of curcumin on lipid peroxide (LPO) level, myeloperoxidase (MPO) and urease activity, number of colonized bacteria, levels of anti-H...
December 2016: Annals of Translational Medicine
https://www.readbyqxmd.com/read/28143992/an-east-asian-type-caga-helicobacter-pylori-infected-patient-with-clinical-manifestation-of-gastric-ulcer
#8
Yudith Annisa Ayu Rezkitha, Muhammad Miftahussurur, Iswan A Nusi, Ummi Maimunah, Pangestu Adi, Yoshio Yamaoka
We reported a male, 72 yo, Chinese ethnic with chief complaint black mushy defecation. Physical examination revealed pale on conjunctival palpebra which confirmed as anemia on complete blood count. Gastroduodenoscopy revealed a 3 mm ulcer at the antrum (Forrest stage III). H. pylori infection was positive based on five different test methods (urinary antibody tests, rapid urease test, culture, histology ad immunohistochemistry). Used polymerase chain reaction-based sequencing, we found the patient infected by CagA producing, East-Asian-type cagA and vacA s1m1-strain...
October 2016: Acta Medica Indonesiana
https://www.readbyqxmd.com/read/28138399/rapid-identification-of-helicobacter-pylori-and-assessment-of-clarithromycin-susceptibility-from-clinical-specimens-using-fish
#9
Ebru Demiray-Gürbüz, Özlem Yılmaz, Asalia Z Olivares, Can Gönen, Sülen Sarıoğlu, Müjde Soytürk, Sait Tümer, Oğuz Altungöz, İlkay Şimşek, Guillermo I Perez Perez
Helicobacter pylori remains one of the most common bacterial infections worldwide. Clarithromycin resistance is the most important cause of H. pylori eradication failures. Effective antibiotic therapies in H. pylori infection must be rapidly adapted to local resistance patterns. We investigated the prevalence of clarithromycin resistance due to mutations in positions 2142 and 2143 of 23SrRNA gene of H. pylori by fluorescence in situ hybridisation (FISH), and compared with culture and antimicrobial susceptibility testing in 234 adult patients with dyspepsia who were enrolled...
January 2017: Journal of Pathology. Clinical Research
https://www.readbyqxmd.com/read/28138371/detection-of-helicobacter-pylori-in-the-bronchoalveolar-lavage-of-patients-with-lung-cancer-using-real-time-pcr
#10
Mitra Samareh-Fekri, Seyed Mehdi Hashemi Bajgani, Ahmad Shafahi, Mahbobeh Asadi-Zarandi, Hamid Mollaie, Arshia Jamali Paghalhe
BACKGROUND: Lung cancer is one of the most common causes of death worldwide. Although smoking and environmental pollutants are the most important risk factors of lung cancer, the role of infectious causes should also be considered in the pathogenesis and progress of lung cancer. OBJECTIVES: This study examined the relationship between Helicobacter pylori and lung cancer through serology, real-time PCR, and urease tests. METHODS: This descriptive cross-sectional study was conducted on 52 adult patients with lung cancer who were selected after having their history taken and being physically examined by a pulmonologist...
November 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/28137214/phytochemical-constituents-ches-and-urease-inhibition-antiproliferative-and-antioxidant-properties-of-elaeagnus-umbellata-thunb
#11
Tevfik Ozen, Semiha Yenigun, Muhammed Altun, Ibrahim Demirtas
Due to the common ethnopharmacological used or scientifically examined biochemical properties, Elaeagnaceae family, Elaeagnus umbellata Thunb. (autumn olive) was worth investigated. In this investigation, we revealed antioxidant, antiproliferative and enzyme inhibition activities of the water, methanol, ethanol, acetone, ethyl acetate and hexane extracts of E. umbellata as well as contents of their phenolic, flavonoid, anthocyanin, ascorbic acid, lycopene and b-carotene. The antioxidant activity was screened by phosphomolybdenum assay...
January 27, 2017: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/28133477/early-diagnosis-of-helicobacter-pylori-infection-in-vietnamese-patients-with-acute-peptic-ulcer-bleeding-a-prospective-study
#12
Duc Trong Quach, Mai Ngoc Luu, Toru Hiyama, Thuy-HuongThi To, Quy Nhuan Bui, Tuan Anh Tran, Binh Duy Tran, Minh-Cong Hong Vo, Shinji Tanaka, Naomi Uemura
Aims. To investigate H. pylori infection rate and evaluate a combined set of tests for H. pylori diagnosis in Vietnamese patients with acute peptic ulcer bleeding (PUD). Methods. Consecutive patients with acute PUB were enrolled prospectively. Rapid urease test (RUT) with 3 biopsies was carried out randomly. Patients without RUT or with negative RUT received urea breath test (UBT) and serological and urinary H. pylori antibody tests. H. pylori was considered positive if RUT or any noninvasive test was positive...
2017: Gastroenterology Research and Practice
https://www.readbyqxmd.com/read/28124965/-bacteremia-caused-by-ciprofloxacin-resistant-salmonella-serotype-kentucky-a-case-report-and-the-review-of-literature
#13
Tuba Müderris, Fatma Yekta Ürkmez, Şeref Alp Küçüker, Muhammet Fethi Sağlam, Gül Ruhsar Yılmaz, Rahmet Güner, Revasiye Güleşen, Ziya Cibali Açıkgöz
Salmonella infections can be seen in four clinical types, namely gastroenteritis, bacteremia/sepsis, enteric fever and carriage. These infections can result in uncomplicated diarrhea in most cases, but can lead to invasive disease requiring antimicrobial therapy and can be life-threatening in elderly or immunocomprimised patients. Broad-spectrum cephalosporins and fluoroquinolones are crucial options in the treatment of the invasive infections. Ciprofloxacin resistance is rarely seen in non-typhoid Salmonella enterica isolates, and only in S...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28124660/decreasing-prevalence-of-helicobacter-pylori-according-to-birth-cohorts-in-urban-china
#14
Xinjuan Yu, Xuan Yang, Tingting Yang, Quanjiang Dong, Lili Wang, Lei Feng
BACKGROUND/AIMS: The Helicobacter pylori prevalence has been decreasing in many parts of the world. This study aimed to investigate the current epidemiological status of H. pylori infection in urban China. MATERIALS AND METHODS: The study included 51,299 subjects aged ≥18 years who underwent health checkups between April 2013 and June 2016 in a city of China. H. pylori infection was determined by detecting H. pylori urease-IgG antibodies. Statistical analyses included chi-square tests for trends and curve fitting...
January 26, 2017: Turkish Journal of Gastroenterology: the Official Journal of Turkish Society of Gastroenterology
https://www.readbyqxmd.com/read/28058025/relationship-between-ghrelin-helicobacter-pylori-and-gastric-mucosal-atrophy-in-hemodialysis-patients
#15
Hitomi Ichikawa, Mitsushige Sugimoto, Yukitoshi Sakao, Shu Sahara, Naro Ohashi, Akihiko Kato, Ken Sugimoto, Takahisa Furuta, Akira Andoh, Tadashi Sakao, Hideo Yasuda
AIM: To investigate the relationship between plasma ghrelin level, Helicobacter pylori (H. pylori) infection status and the severity of atrophy in hemodialysis patients. METHODS: One hundred eights patients who received hemodialysis and 13 non-hemodialysis H. pylori-negative controls underwent gastroduodenoscopy to evaluate the severity of gastric atrophy. Serum levels of pepsinogen (PG) were measured as serum markers of gastric atrophy. H. pylori infection was evaluated by anti-H...
December 21, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28054994/synthesis-of-new-nitrofluoroquinolone-derivatives-with-novel-anti-microbial-properties-against-metronidazole-resistant-h-pylori
#16
Luay Abu-Qatouseh, Mohammad Abu-Sini, Amal Mayyas, Yusuf Al-Hiari, Rula Darwish, Talal Aburjai
One of the major therapeutic approaches to preventing relapse and accelerating the healing of duodenal and gastric ulcers is the eradication of Helicobacter pylori. Due to the emergence of antibiotic resistance among clinical strains of H. pylori, alternative approaches using newly discovered antimicrobial agents in combination with the standard regimens for the treatment of H. pylori are increasingly needed. The purpose of the present study was to investigate the effect of newly synthesized 8-nitroflouroqunolone derivatives when used either alone or when combined with metronidazole against metronidazole-resistant H...
January 4, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28049442/analyzing-the-influence-of-gastric-intestinal-metaplasia-on-gastric-ulcer-healing-in-helicobacter-pylori-infected-patients-without-atrophic-gastritis
#17
COMPARATIVE STUDY
Li-Wei Chen, Liang-Che Chang, Chung-Ching Hua, Bor-Jen Hsieh, Shuo-Wei Chen, Rong-Nan Chien
BACKGROUND: Gastric epithelial hyper-proliferation was reported in patients with Helicobacter pylori (H. pylori)-infected gastric mucosa with intestinal metaplasia (IM) changes. In patients with gastric ulcer (GU) and IM, the GU may have a different healing rate in comparison to patients without IM. This study aimed to compare the difference in GU healing between H. pylori-infected patients with IM and those without IM. METHODS: We retrospectively analyzed patients at the Keelung Chung Gung Memorial Hospital during the period from March 2005 to January 2011...
January 3, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/28045966/inhibition-of-helicobacter-pylori-and-its-associated-urease-by-palmatine-investigation-on-the-potential-mechanism
#18
Jiang-Tao Zhou, Cai-Lan Li, Li-Hua Tan, Yi-Fei Xu, Yu-Hong Liu, Zhi-Zhun Mo, Yao-Xing Dou, Rui Su, Zi-Ren Su, Ping Huang, Jian-Hui Xie
In this paper, we evaluated the anti-Helicobacter pylori activity and the possible inhibitory effect on its associated urease by Palmatine (Pal) from Coptis chinensis, and explored the potential underlying mechanism. Results indicated that Pal exerted inhibitory effect on four tested H. pylori strains (ATCC 43504, NCTC 26695, SS1 and ICDC 111001) by the agar dilution test with minimum inhibitory concentration (MIC) values ranging from 100 to 200 μg/mL under neutral environment (pH 7.4), and from 75 to 100 μg/mL under acidic conditions (pH 5...
2017: PloS One
https://www.readbyqxmd.com/read/28042765/study-of-serum-levels-of-some-oxidative-stress-markers-in-children-with-helicobacter-pylori-infection
#19
Adel A Hagag, Saleh M Amin, Rasha B El-Fiky, Magda E El-Sayad
BACKGROUND: Helicobacter pylori are gram-negative spiral shaped bacteria, with sheathed flagella. H. pylori infection is one of the most common chronic infections in humans. Infection is usually acquired during childhood, and becomes a lifelong infection in most people unless treated. The aim of this study was to evaluate serum levels of oxidative stress indices in children with H. pylori infection. MATERIAL AND METHODS: The present study was carried out on 60 children infected with H...
January 2, 2017: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/28033692/helicobacter-pylori-and-its-virulence-factors-effect-on-serum-oxidative-dna-damages-in-adults-with-dyspepsia
#20
Heshmat Shahi, Rasoul Bahreiny, Somayeh Reiisi
Helicobacter Pylori infection is a common gastrointestinal infection that can cause pathological effects, increase oxidative stress and induce an inflammatory response in gastric mucosa. Inflammatory aspects may prompt the production of radical oxygen substance (ROS) which may damage cells and release 8-hydroxydyoxyguanosine (8-OHdG) to serum. In this study, we evaluate the prevalence of H. pylori virulence factors and the association between serum level of 8-OHdG, H. pylori infection, and its various virulence factors...
November 2016: Acta Medica Iranica
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