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Urease test

Humayun Pervez, Nazia Khan, Jamshed Iqbal, Sumera Zaib, Muhammad Yaqub, Muhammad Moazzam Naseer
A series of fifteen N4-benzyl substituted 5-chloroisatin-3-thiosemicarbazones 5a-o were synthesized and screened mainly for their antiurease and antiglycation effects. Lemna aequinocitalis growth and Artemia salina assays were carried out to determine their phytotoxicity and cytotoxicity potential. All the compounds proved to be extremely effective urease inhibitors, demonstrating enzyme inhibition much better than the reference inhibitor, thiourea (IC50 values 1.31 ± 0.06 to 3.24 ± 0.15 vs. 22.3 ± 1.12 μM)...
March 2018: Acta Chimica Slovenica
Mindy Elizabeth Hair, Adrianna I Mathis, Erica K Brunelle, Lenka Halámková, Jan Halámek
Sweat is a biological fluid present on the skin surface of every individual and is known to contain amino acids as well as other low molecular weight compounds. Each individual is inherently different from one another based on certain factors including, but not limited to, his/her genetic make-up, environment, and lifestyle. As such, the biochemical composition of each person greatly differs. The concentrations of the biochemical content within an individual's sweat are largely controlled by metabolic processes within the body that fluctuate regularly based on attributes such as age, gender, and activity levels...
March 21, 2018: Analytical Chemistry
Qusay Abdoh, Lubna Kharraz, Khubaib Ayoub, Jana Khraim, Wafaa Awad, Aysha Sbeah, Safaa Turman
BACKGROUND: Bacterial resistance to antibiotics is considered the most important determinant of treatment failure. Monitoring the evolution of antimicrobial resistance to common antibiotics is therefore of special importance for clinicians. The frequency of resistance to antibiotics in Helicobacter pylori isolates is increasing. The aim of this study was to determine the pattern of H pylori antibiotic resistance at the An-Najah National University Hospital. METHODS: In this cross-sectional study, we recruited patients older than 18 year who were admitted to the An-Najah National University Hospital...
February 21, 2018: Lancet
Tatsuya Tominaga
Bacteria of the genera Klebsiella and Raoultella, which are present in foods and the natural environment, are associated with health hazards in humans. In the present study, two types of strips-based methods were developed to detect these bacteria simply and quickly. One method used lateral-flow test strips (LFTS) in combination with anti-Klebsiella antibodies labeled with palladium nanoparticles that bind to target bacteria, allowing their visualization. In the other, the antibodies were immobilized on nitrocellulose membranes, and urease activity was measured using an ion sensitive field effect transistor pH sensor...
March 6, 2018: Journal of Microbiological Methods
Alzira Maria de Castro Barbosa, Rosangela Albuquerque Ribeiro, Cícero Ígor Simões Moura Silva, Francisco Will Saraiva Cruz, Orleancio Gomes Ripardo de Azevedo, Maria Helena da Silva Pitombeira, Lucia Libanez Campelo Braga
BACKGROUND: Several studies have demonstrated that platelet counts in Helicobacter pylori-positive patients with chronic idiopathic thrombocytopenic purpura improved significantly after successful eradication of the infection. However, depending of the geographical region of the study the results have been highly divergent. OBJECTIVE: The purpose of this study was to evaluate the effect of H. pylori eradication therapy on platelet count in a cohort of chronic idiopathic thrombocytopenic purpura patients from northeastern Brazil...
January 2018: Revista Brasileira de Hematologia e Hemoterapia
Min Chen, Jian Chen, Yue Yang, Lei Cheng, Hai-Tao Wu
BACKGROUND: Several studies have indicated the larynx as possible Helicobacter pylori (H. pylori) reservoirs. This study explored the association between H. pylori and vocal fold leukoplakia. METHODS: The case-control study involved 51 patients with vocal fold leukoplakia and 35 control patients with vocal polyps. Helicobacter pylori was detected in tissues by the rapid urease test, nested polymerase chain reaction (PCR), and single-step PCR. The H. pylori-specific immunoglobulin antibodies were detected in plasma by enzyme-linked immunosorbent assay (ELISA)...
March 6, 2018: Head & Neck
Minyoung Lee, Haeri Baek, Jong Suk Park, Sohee Kim, Chanhee Kyung, Su Jung Baik, Byoung Kwon Lee, Jie-Hyun Kim, Chul Woo Ahn, Kyung Rae Kim, Shinae Kang
Helicobacter pylori is a gastrointestinal pathogen known to be associated with cardiovascular disease (CVD). However, most analyses about the effect of H. pylori infection have been done in patients with a history of CVD but not in healthy subjects. We evaluated the association between H. pylori infection and subclinical atherosclerosis by using cardiac multidetector computed tomography (MDCT) in healthy subjects without previous CVD. From December 2007 to February 2014, 463 subjects who underwent the rapid urease test (CLO test), pulse-wave velocity (PWV) measurement, and MDCT for a self-referred health check-up were enrolled to this study...
2018: PloS One
Chengrong Nie, Xing Yang, Nabeel Khan Niazi, Xiaoya Xu, Yuhui Wen, Jörg Rinklebe, Yong Sik Ok, Song Xu, Hailong Wang
In the current study, we conducted a field experiment using the test plant, Brassica chinesis L. (pak choi), to investigate the effect of sugarcane bagasse-derived biochar on the bioavailability of cadmium (Cd), copper (Cu) and lead (Pb), and the health of soil microbiota in a contaminated soil. Biochar application significantly (P < 0.05) increased pak choi yield. Bioavailability of heavy metals to plant shoots and roots decreased with increasing biochar application rates (at 0, 1.5, 2.25 and 3.0 t ha-1 )...
February 22, 2018: Chemosphere
Tran Thanh Binh, Vo Phuoc Tuan, Ho Dang Quy Dung, Pham Huu Tung, Tran Dinh Tri, Ngo Phuong Minh Thuan, Le Quang Tam, Bui Chi Nam, Do Anh Giang, Phan Quoc Hoan, Tomohisa Uchida, Tran Thi Huyen Trang, Vu Van Khien, Yoshio Yamaoka
The Helicobacter pylori -induced burden of gastric cancer varies based on geographical regions and ethnic grouping. Vietnam is a multiethnic country with the highest incidence of gastric cancer in Southeast Asia, but previous studies focused only on the Kinh ethnic group. A population-based cross-sectional study was conducted using 494 volunteers (18-78 years old), from 13 ethnic groups in Daklak and Lao Cai provinces, Vietnam. H. pylori status was determined by multiple tests (rapid urease test, culture, histology, and serology)...
March 1, 2018: International Journal of Molecular Sciences
Mehdi Tourani, Maryam Habibzadeh, Ahmad Karkhah, Javad Shokri-Shirvani, Ladan Barari, Hamid Reza Nouri
Peptic ulcer is a lesion in the mucosa of the digestive tract affecting many people all around the world. Recent investigations have indicated that produced inflammatory cytokines such as TNF-α and IL-1β in response to gastric infection by Helicobacter pylori play an important role in the development of peptic ulcer. With regard to the significance of these cytokines in peptic ulcer development and the high prevalence of this disease in the developing countries, this study aimed to investigate the association of TNF-α and IL-1β with peptic ulcer in the presence of H...
February 15, 2018: Cytokine
Abdul Wahab, Syed Aleem Jan, Abdur Rauf, Zia Ur Rehman, Zahid Khan, Aftab Ahmed, Fatima Syed, Sher Zaman Safi, Hamayun Khan, Muhammad Imran
OBJECTIVE: Scandix pecten-veneris L. is a less studied wild edible herb and is considered an extinct plant species in many parts of the world. This study was designed to evaluate its phytochemical composition and biological potential of S. pecten-veneris L. METHODS: Phytochemicals including alkaloids, flavonoids, polyphenols, and tannins were determined in extracts of S. pecten-veneris. Antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), while reducing power was tested by ferric reducing/antioxidant power (FRAP) assay...
2018: Journal of Zhejiang University. Science. B
Verima Pereira, Philip Abraham, Sivaramaiah Nallapeta, Anjali Shetty
BACKGROUND: The gastric microbiota has recently been implicated in the causation of organic/structural gastroduodenal diseases (gastric and duodenal ulcers, gastric cancer) in patients with Helicobacter pylori (H. pylori) infection. We aimed to ascertain, in patients harbouring H. pylori, the role of the gastric microbiota in the causation of symptoms (chronic dyspepsia) in the absence of organic disease. METHODS: Seventy-four gastric biopsy samples obtained at endoscopy from patients with (n = 21) or without (n = 53) chronic dyspepsia, and that tested positive by the bedside rapid urease test for H...
January 26, 2018: BMC Gastroenterology
Amit K Dutta, Viswanath D Reddy, Venkatkrishnan H Iyer, L S Unnikrishnan, Ashok Chacko
Recent data from Asian countries including India has shown a significant decline in the frequency of peptic ulcer disease (PUD) compared to the past. H. pylori is considered the most important risk factor for PUD, and we aimed to explore the current frequency of H. pylori infection in different age groups of patients with dyspepsia. Patients >15 years of age with dyspeptic symptoms were prospectively recruited in this study from 2010 to 2014 after obtaining informed consent. Patients were divided into three age groups: 15-30 years, 31-50 years, and >50 years, and the minimum sample size required in the three groups with a power of 90% was 259, 256, and 188, respectively...
January 24, 2018: Indian Journal of Gastroenterology: Official Journal of the Indian Society of Gastroenterology
Sakshi Kohli, Vikram K Mahajan, Baldev S Rana, Karaninder S Mehta, Rashmi Kaul Raina, Pushpinder S Chauhan, Vikas Sharma, Ritu Rawat
BACKGROUND: The Helicobacter pylori infection is linked to chronic urticaria in nearly 60% of patients. We studied clinicoepidemiologic features in patients with chronic urticaria with and without H. pylori infection. METHODS: Endoscopic antral biopsy for the rapid urease test (RUT) and histopathology, and serum IgG ELISA for H. pylori infection were performed in 150 patients (male:female ratio 1:2.4) of chronic urticaria aged 18-68 years. Clinicoepidemiologic features including age, gender, age of onset and duration, frequency and distribution of wheals, urticaria severity score, and systemic symptoms were analyzed in patients with and without H...
January 19, 2018: International Archives of Allergy and Immunology
Yooeun Chae, Dokyung Kim, Youn-Joo An
Fluorine can flow into the environment after leakage or spill accidents and these excessive amounts can cause adverse effects on terrestrial ecosystems. Using three media (filter paper, soil, and filter-paper-on-soil), we investigated the toxic effects of fluorine on the germination and growth of crops (barley, mung bean, sorghum, and wheat), on the activities of soil exoenzymes (acid phosphatase, arylsulfatase, fluorescein diacetate hydrolase, and urease) and on the survival, abnormality, and cytotoxicity of Eisenia andrei earthworms...
January 2, 2018: Ecotoxicology and Environmental Safety
Vignesh Shetty, Mamatha Ballal, Girisha Balaraju, Shiran Shetty, Ganesh C Pai, Ramachandra Lingadakai
Background: Helicobacter pylori affects almost half of the world's population and therefore is one of the most frequent and persistent bacterial infections worldwide. H. pylori is associated with chronic gastritis, ulcer disease (gastric and duodenal), mucosa-associated lymphoid tissue lymphoma, and gastric cancer. Several diagnostic methods exist to detect infection and the option of one method or another depends on various genes, such as availability, advantages and disadvantages of each method, monetary value, and the age of patients...
October 2017: Journal of Global Infectious Diseases
Adianez Sugrañes-Montalván, Eduardo Barreto-Suárez, Elizabeth Nicolau-Pestana, Lidyce Quesada-Leyva
BACKGROUND: Chronic spontaneous urticaria is a frequent disease that affects the quality of life of patients. Helicobacter pylori infection has increased in the world, linking current studies with a link between both entities. OBJECTIVE: To determine the relationship between chronic urticaria and Helicobacter pylori infection. METHODS: An observational cohort study was conducted with the objective of demonstrating the relationship between chronic urticaria and Helicobacter pylori infection in the allergology clinic of the Hospital Universitario "Manuel Ascunce Domenech" in the province of Camagüey, Cuba, in the period from January 2015 to December of 2016...
October 2017: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
Masahiro Yasunishi, Akihiro Koumura, Yuichi Hayashi, Shohei Nishida, Takashi Inuzuka
A 71-year-old woman with a 9-year history of Parkinson's disease was admitted to our hospital emergently because of consciousness disturbance. Her consciousness level was 200 on the Japan coma scale (JCS), and she presented with tenderness and distension of the lower abdomen. Brain computed tomography showed normal findings. Blood tests showed an increased ammonia level (209 μg/dl) with normal AST and ALT levels. We catheterized the bladder for urinary retention. Five hours after admission, the blood ammonia level decreased to 38 μg/dl, and her consciousness level improved dramatically...
2017: Nihon Ronen Igakkai Zasshi. Japanese Journal of Geriatrics
Gokben Ozbey, Alfizah Hanafiah
H. pylori infection is a global public health problem associated with some gastrointestinal diseases in children, especially in developing countries, since prevalence of H. pylori is low in the developed world. Both noninvasive (stool antigen test, urea breath test, and blood test) and invasive (histology, rapid urease test, and microbiological culture) tests have been utilized to detect H. pylori infection. However, a single test is not reliable enough and does not provide accurate enough data to determine H...
January 2017: Euroasian Journal of Hepato-Gastroenterology
Gany Beer-Davidson, Musa Hindiyeh, Khitam Muhsen
BACKGROUND: The aims of this study were to develop and validate a multiplex real-time polymerase chain reaction (q-PCR) assay of Helicobacter pylori in stool samples of healthy children. Additionally, we determined the prevalence of clarithromycin resistance and cagA gene in H. pylori-positive samples. MATERIALS AND METHODS: Archived stool samples from 188 children aged 6-9 years and 272 samples of 92 infants aged 2-18 months were tested for H. pylori antigens using enzyme immunoassay (EIA)...
February 2018: Helicobacter
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