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https://www.readbyqxmd.com/read/28728562/low-oxygen-tension-increases-mitochondrial-membrane-potential-and-enhances-expression-of-antioxidant-genes-and-implantation-protein-of-mouse-blastocyst-cultured-in-vitro
#1
Yun-Yi Ma, Huei-Wen Chen, Chii-Ruey Tzeng
BACKGROUND: In human IVF, embryos cultured with a lower O2 tension (5%) can give rise to higher success rates when compared with normoxic conditions (20%). However, the mechanisms behind the beneficial effects of reduced oxygen tension in embryogenesis remain unclear. The aim of this study was to evaluate the expression of oxygen related and antioxidant genes and mitochondrial function in mouse embryo cultured under hypoxic and normoxic conditions, to investigate the beneficial effect of low oxygen tension in preimplantation embryogenesis...
July 20, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28728132/melatonin-protect-the-development-of-preimplantation-mouse-embryos-from-sodium-fluoride-induced-oxidative-injury
#2
Jiamin Zhao, Beibei Fu, Wei Peng, Tingchao Mao, Haibo Wu, Yong Zhang
Recently study shows that melatonin can protect embryos from the culture environment oxidative stress. However, the protective effect of melatonin on the mouse development of preimplantation embryos under sodium fluoride (NaF) induced oxidative stress is still unclear. Here, we showed that exposure to NaF significantly increased the reactive oxygen species (ROS) level, decreased the blastocyst formation rates, and increased the fragmentation, apoptosis and retardation of blastocysts in the development of mouse preimplantation embryos...
June 29, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28725950/genome-editing-and-assisted-reproduction-curing-embryos-society-or-prospective-parents
#3
Giulia Cavaliere
This paper explores the ethics of introducing genome-editing technologies as a new reproductive option. In particular, it focuses on whether genome editing can be considered a morally valuable alternative to preimplantation genetic diagnosis (PGD). Two arguments against the use of genome editing in reproduction are analysed, namely safety concerns and germline modification. These arguments are then contrasted with arguments in favour of genome editing, in particular with the argument of the child's welfare and the argument of parental reproductive autonomy...
July 19, 2017: Medicine, Health Care, and Philosophy
https://www.readbyqxmd.com/read/28723896/maternal-h3k27me3-controls-dna-methylation-independent-imprinting
#4
Azusa Inoue, Lan Jiang, Falong Lu, Tsukasa Suzuki, Yi Zhang
Mammalian sperm and oocytes have different epigenetic landscapes and are organized in different fashions. After fertilization, the initially distinct parental epigenomes become largely equalized with the exception of certain loci, including imprinting control regions. How parental chromatin becomes equalized and how imprinting control regions escape from this reprogramming is largely unknown. Here we profile parental allele-specific DNase I hypersensitive sites in mouse zygotes and morula embryos, and investigate the epigenetic mechanisms underlying these allelic sites...
July 19, 2017: Nature
https://www.readbyqxmd.com/read/28722155/metabolite-availability-as-a-window-to-view-the-early-embryo-microenvironment-in-vivo
#5
REVIEW
Kailun Hu, Yang Yu
A preimplantation embryo exists independent of blood supply, and relies on energy sources from its in vivo environment (e.g. oviduct and uterine fluid) to sustain its development. The embryos can survive in this aqueous environment because it contains amino acids, proteins, lactate, pyruvate, oxygen, glucose, antioxidants, ions, growth factors, hormones, and phospholipids - albeit the concentration of each component varies by species, stage of the estrous cycle, and anatomical location. The dynamic nature of this environment sustains the early development from the one-cell zygote to blastocyst, and is reciprocally influenced by the embryo at each embryonic stage...
July 19, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28720156/fmr1-cgg-repeat-expansion-mutation-detection-and-linked-haplotype-analysis-for-reliable-and-accurate-preimplantation-genetic-diagnosis-of-fragile-x-syndrome
#6
Indhu-Shree Rajan-Babu, Mulias Lian, Felicia S H Cheah, Min Chen, Arnold S C Tan, Ethiraj B Prasath, Seong Feei Loh, Samuel S Chong
Fragile X mental retardation 1 (FMR1) full-mutation expansion causes fragile X syndrome. Trans-generational fragile X syndrome transmission can be avoided by preimplantation genetic diagnosis (PGD). We describe a robust PGD strategy that can be applied to virtually any couple at risk of transmitting fragile X syndrome. This novel strategy utilises whole-genome amplification, followed by triplet-primed polymerase chain reaction (TP-PCR) for robust detection of expanded FMR1 alleles, in parallel with linked multi-marker haplotype analysis of 13 highly polymorphic microsatellite markers located within 1 Mb of the FMR1 CGG repeat, and the AMELX/Y dimorphism for gender identification...
July 19, 2017: Expert Reviews in Molecular Medicine
https://www.readbyqxmd.com/read/28719901/patient-related-benefits-for-adults-with-cochlear-implantation-a-multicultural-longitudinal-observational-study
#7
Thomas Lenarz, Lida Muller, Hanna Czerniejewska-Wolska, Hector Vallés Varela, César Orús Dotú, Marcin Durko, Alicia Huarte Irujo, Bartosz Piszczatowski, Marek Zadrożniak, Colin Irwin, Petra L Graham, Josie Wyss
OBJECTIVES: To assess subjectively perceived, real-world benefits longitudinally for unilateral cochlear implant (CI) recipients in a multinational population treated routinely. To identify possible predictors of self-reported benefits. DESIGN: This was a prospective, multicenter, repeated-measures study. Self-assessment of performance at preimplantation and postimplantation at 1, 2, and 3 years using standardized, validated, local language versions of the Speech, Spatial, and Qualities of Hearing Scale (SSQ), and the Health Utilities Index Mark 3 (HUI3) was performed...
July 19, 2017: Audiology & Neuro-otology
https://www.readbyqxmd.com/read/28719345/h2o2-induced-mild-stress-in-relation-with-in-vitro-ovine-oocyte-developmental-competence-implications-for-blastocyst-apoptosis-and-related-genes-expression
#8
K Nikdel, M Aminafshar, A Mohammadi-Sangcheshmeh, N EmamJomeh-Kashan, E Seyedjafari
In this study, in vitro maturation was performed in presence of various concentrations (0, 10, 100, or 1000 µM) of H2O2. The intracellular glutathione (GSH) level, fertilization, cleavage, and blastocyst rates, total cell number, and apoptotic cell number and expression of Bax, Bcl-2, and p53 genes in blastocyst-stage embryos were studied. At 10 μM H2O2 concentration, a higher GSH level was detected in comparison to the other groups while oocytes exposed to 1000 μM H2O2 had the lowest GSH level. Treatment of oocytes with 1000 μM H2O2 decreased the rate of two pronuclei formation as compared with other groups...
May 20, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28718081/blastocyst-utilization-rates-after-continuous-culture-in-two-commercial-single-step-media-a-prospective-randomized-study-with-sibling-oocytes
#9
Ioannis A Sfontouris, Efstratios M Kolibianakis, George T Lainas, Christos A Venetis, George K Petsas, Basil C Tarlatzis, Tryfon G Lainas
PURPOSE: The aim of this study is to determine whether blastocyst utilization rates are different after continuous culture in two different commercial single-step media. METHODS: This is a paired randomized controlled trial with sibling oocytes conducted in infertility patients, aged ≤40 years with ≥10 oocytes retrieved assigned to blastocyst culture and transfer. Retrieved oocytes were randomly allocated to continuous culture in either Sage one-step medium (Origio) or Continuous Single Culture (CSC) medium (Irvine Scientific) without medium renewal up to day 5 post oocyte retrieval...
July 17, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28717645/whole-genome-amplification-of-day-3-or-day-5-human-embryos-biopsies-provides-a-suitable-dna-template-for-pcr-based-techniques-for-genotyping-a-complement-of-preimplantation-genetic-testing
#10
Elizabeth Schaeffer, Bruno López-Bayghen, Adina Neumann, Leonardo M Porchia, Rafael Camacho, Efraín Garrido, Rocío Gómez, Felipe Camargo, Esther López-Bayghen
Our objective was to determine if whole genome amplification (WGA) provides suitable DNA for qPCR-based genotyping for human embryos. Single blastomeres (Day 3) or trophoblastic cells (Day 5) were isolated from 342 embryos for WGA. Comparative Genomic Hybridization determined embryo sex as well as Trisomy 18 or Trisomy 21. To determine the embryo's sex, qPCR melting curve analysis for SRY and DYS14 was used. Logistic regression indicated a 4.4%, 57.1%, or 98.8% probability of a male embryo when neither gene, SRY only, or both genes were detected, respectively (accuracy = 94...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28712378/treatment-of-allicin-improves-maturation-of-immature-oocytes-and-subsequent-developmental-ability-of-preimplantation-embryos
#11
Sang-Gi Jeong, Seung-Eun Lee, Yun-Gwi Park, Yeo-Jin Son, Min-Young Shin, Eun-Young Kim, Se-Pill Park
Allicin (AL) regulates the cellular redox, proliferation, viability, and cell cycle of different cells against extracellular-derived stress. This study investigated the effects of allicin treatment on porcine oocyte maturation and developmental competence. Porcine oocytes were cultured in medium supplemented with 0 (control), 0.01, 0.1, 1, 10 or 100 μM AL, respectively, during in vitro maturation (IVM). The rate of polar body emission was higher in the 0.1 AL-treated group (74.5% ± 2.3%) than in the control (68...
July 17, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28711945/the-molecular-characterization-of-porcine-egg-precursor-cells
#12
Te-Sha Tsai, Jacqueline Johnson, Yvonne White, Justin C St John
Female-factor infertility can be caused by poor oocyte quality and depleted ovarian reserves. Egg precursor cells (EPCs), isolated from the ovarian cortex, have the potential to be used to overcome female infertility. We aimed to define the origins of EPCs by analyzing their gene expression profiles and mtDNA content using a mini-pig model. We characterized FAC-sorted DDX4+-derived porcine EPCs by performing RNA-sequencing and determined that they utilize pathways important for cell cycle and proliferation, which supports the existence of adult mitotically active oogonial cells...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28711491/copper-chaperone-atox1-regulates-pluripotency-factor-oct4-in-preimplantation-mouse-embryos
#13
Emanuele Celauro, Amisa Mukaj, Juan Carlos Fierro-González, Pernilla Wittung-Stafshede
Despite of the importance of copper (Cu) during pregnancy, the roles of Cu-binding proteins during early embryonic development are unknown. The Cu chaperone ATOX1 was recently suggested to have additional functions related to transcription and cancer. When we analyzed single-cell RNA transcript data from early mouse embryos, Atox1 transcript levels increased dramatically at the 8-cell stage and, at 16- and 32-cell embryo stages, matched those of Oct4 which expresses a transcription factor essential for pluripotency in the inner cell mass...
July 12, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28708489/2-methoxystypandrone-improves-in%C3%A2-vitro-produced-bovine-embryo-quality-through-inhibition-of-ikbkb
#14
Ayman Mesalam, Imran Khan, Kyeong-Lim Lee, Seok-Hwan Song, M M R Chowdhury, Zia Uddin, Ki Hun Park, Il-Keun Kong
2-Methoxystypandrone (2-MS), a naphthoquinone from Polygonum cuspidatum, has been reported to exhibit antioxidant activities. However, its pharmacological properties have not been fully studied in vitro culture system of oocytes and embryos development. In this study, we examined the effects of 2-MS on bovine blastocyst developmental competency during preimplantation using TUNEL assay, qRT-PCR and immunocytochemistry. Oocytes were treated with 1 μM 2-MS during maturation, followed by in vitro fertilized and the zygotes were cultured...
May 19, 2017: Theriogenology
https://www.readbyqxmd.com/read/28706983/preimplantation-kidney-biopsies-of-extended-criteria-donors-have-a-heavier-inflammatory-burden-than-kidneys-from-standard-criteria-donors
#15
Camila M Mazeti-Felicio, Heloisa C Caldas, Ida M M Fernandes-Charpiot, Camila Z Dezotti, Maria A S F Baptista, Mario Abbud-Filho
BACKGROUND: Donors after brain death develop a systemic proinflammatory state that may predispose the kidneys to injury after transplantation. Because it is not known whether this inflammatory environment similarly affects the kidneys from expanded criteria donor (ECD) and standard criteria donors (SCD), we sought to evaluate differences in the gene expression of inflammatory cytokines in preimplantation biopsies (PIBx) from ECD and SCD kidneys. METHODS: Cytokines gene expression was measured in 80 PIBx (SCD, 52; ECD, 28) and associated with donor variables...
July 2017: Transplantation Direct
https://www.readbyqxmd.com/read/28704412/synthetic-preimplantation-factor-pif-prevents-fetal-loss-by-modulating-lps-induced-inflammatory-response
#16
Nicoletta Di Simone, Fiorella Di Nicuolo, Riccardo Marana, Roberta Castellani, Francesco Ria, Manuela Veglia, Giovanni Scambia, Daniel Surbek, Eytan Barnea, Martin Mueller
Maternal control of inflammation is essential during pregnancy and an exaggerated response is one of the underlying causes of fetal loss. Inflammatory response is mediated by multiple factors and Toll-like receptors (TLRs) are central. Activation of TLRs results in NALP-3 mediated assembly of apoptosis-associated speck-like protein containing a CARD (ASC) and caspase-1 into the inflammasome and production of pro-inflammatory cytokines IL-1β and IL-18. Given that preventing measures are lacking, we investigated PreImplantation Factor (PIF) as therapeutic option as PIF modulates Inflammation in pregnancy...
2017: PloS One
https://www.readbyqxmd.com/read/28703188/allelic-reprogramming-of-3d-chromatin-architecture-during-early-mammalian-development
#17
Zhenhai Du, Hui Zheng, Bo Huang, Rui Ma, Jingyi Wu, Xianglin Zhang, Jing He, Yunlong Xiang, Qiujun Wang, Yuanyuan Li, Jing Ma, Xu Zhang, Ke Zhang, Yang Wang, Michael Q Zhang, Juntao Gao, Jesse R Dixon, Xiaowo Wang, Jianyang Zeng, Wei Xie
In mammals, chromatin organization undergoes drastic reprogramming after fertilization. However, the three-dimensional structure of chromatin and its reprogramming in preimplantation development remain poorly understood. Here, by developing a low-input Hi-C (genome-wide chromosome conformation capture) approach, we examined the reprogramming of chromatin organization during early development in mice. We found that oocytes in metaphase II show homogeneous chromatin folding that lacks detectable topologically associating domains (TADs) and chromatin compartments...
July 12, 2017: Nature
https://www.readbyqxmd.com/read/28700688/four-simple-rules-that-are-sufficient-to-generate-the-mammalian-blastocyst
#18
Silas Boye Nissen, Marta Perera, Javier Martin Gonzalez, Sophie M Morgani, Mogens H Jensen, Kim Sneppen, Joshua M Brickman, Ala Trusina
Early mammalian development is both highly regulative and self-organizing. It involves the interplay of cell position, predetermined gene regulatory networks, and environmental interactions to generate the physical arrangement of the blastocyst with precise timing. However, this process occurs in the absence of maternal information and in the presence of transcriptional stochasticity. How does the preimplantation embryo ensure robust, reproducible development in this context? It utilizes a versatile toolbox that includes complex intracellular networks coupled to cell-cell communication, segregation by differential adhesion, and apoptosis...
July 2017: PLoS Biology
https://www.readbyqxmd.com/read/28697911/preimplantation-genetic-screening-what-is-the-clinical-efficiency
#19
EDITORIAL
Richard J Paulson
No abstract text is available yet for this article.
July 8, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28696244/effect-of-vitrification-on-in-vitro-development-and-imprinted-gene-grb10-in-mouse-embryos
#20
Jianfeng Yao, Lixia Geng, Rongfu Huang, Weilin Peng, Xuan Chen, Xiaohong Jiang, Miao Yu, Ming Li, Yanfang Huang, Xiaoyu Yang
Vitrification of embryos is a routine procedure in IVF (in vitro fertilization) laboratories. In the present study, we aimed to investigate the effect of vitrification on mouse preimplantation embryo development in vitro, and effect on the epigenetic status of imprinted gene Grb10 in mouse embryos. The blastocyst formation rate for vitrified 8-cell embryos was similar to the non-vitrified 8-cell embryos, whereas the blastocyst hatching rate was lower than that of the non-vitrified group. The expression level of Grb10 major-type transcript decreased significantly in vitrified blastocysts compared with non-vitrified and in vivo blastocysts...
September 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
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