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Munusamy Madhaiyan, Selvaraj Poonguzhali, Venkatakrishnan Sivaraj Saravanan, Veeramuthu Duraipandiyan, Naif Abdullah Al-Dhabi, Dhandapani Pragatheswari, Palani Santhanakrishnan, Soon-Jin Kim, Hang-Yeon Weon, Soon-Wo Kwon
A novel siderophore-producing actinomycete, designated PL19T, was isolated from the Scots-pine needles collected from TNAU campus, Coimbatore, India. The isolate was recovered as chemoorganotrophic in nutrition and able to show favorable growth at 30°C, and the optimum pH and NaCl level facilitated the growth includes 6-11 and 0-8% (w/v) respectively. The cells are filamentous and the mycelia formed are basically of wide and intricately branched substrate mycelium from which aerial mycelia arises, which latter differentiate into spores that were warty and arranged spirally...
July 27, 2016: International Journal of Systematic and Evolutionary Microbiology
Christian Besler, Daniel Urban, Stefan Watzka, David Lang, Karl-Philipp Rommel, Reinhard Kandolf, Karin Klingel, Holger Thiele, Axel Linke, Gerhard Schuler, Volker Adams, Philipp Lurz
AIMS: Inflammatory heart disease represents an important cause of chronic dilated cardiomyopathy (DCM). Predicting the clinical course of patients with inflammatory cardiomyopathy (iCMP) is difficult, and the prognostic value of current biological markers remains controversial. We tested whether expression of selected microRNAs in endomyocardial biopsies (EMBs) is related to LV functional recovery and clinical events in iCMP patients. METHODS AND RESULTS: EMBs were obtained from patients with iCMP (n = 76) and non-inflammatory DCM (n = 22)...
June 13, 2016: European Journal of Heart Failure
Dieter Dauwe, Beatriz Pelacho, Arief Wibowo, Ann-Sophie Walravens, Kristoff Verdonck, Hilde Gillijns, Ellen Caluwe, Peter Pokreisz, Nick van Gastel, Geert Carmeliet, Maarten Depypere, Frederik Maes, Nina Vanden Driessche, Walter Droogne, Johan Van Cleemput, Johan Vanhaecke, Felipe Prosper, Catherine Verfaillie, Aernout Luttun, Stefan Janssens
BACKGROUND: Blood outgrowth endothelial cells (BOECs) mediate therapeutic neovascularization in experimental models, but outgrowth characteristics and functionality of BOECs from patients with ischemic cardiomyopathy (ICMP) are unknown. We compared outgrowth efficiency and in vitro and in vivo functionality of BOECs derived from ICMP with BOECs from age-matched (ACON) and healthy young (CON) controls. METHODS AND RESULTS: We isolated 3.6±0.6 BOEC colonies/100×10(6) mononuclear cells (MNCs) from 60-mL blood samples of ICMP patients (n=45; age: 66±1 years; LVEF: 31±2%) versus 3...
April 2016: Journal of the American Heart Association
Ben Corteville, Jan De Pooter, Tine De Backer, Milad El Haddad, Roland Stroobandt, Frank Timmermans
AIMS: In patients with systolic heart failure and left bundle branch block (LBBB), septal flash (SF) movement has been described by echocardiography. We evaluated the prevalence of SF in LBBB and non-LBBB patients and evaluated whether specific electrocardiographic (ECG) characteristics within LBBB are associated with the presence of SF on echocardiography. METHODS AND RESULTS: One hundred and four patients with probable LBBB on standard 12-lead ECG were selected, 40 patients with non-LBBB served as controls...
February 2, 2016: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
Sofie E De Meyer, Heng Wee Tan, Mitchell Andrews, Peter B Heenan, Anne Willems
In total, 31 strains of gram-negative, rod-shaped bacteria were isolated from Sophora root nodules and authenticated as rhizobia on this hos. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium, with the representative strains ICMP 19560T, ICMP 19523T, ICMP 19535T, ICMP 19545T and ICMP 19512T being most closely related to Mesorhizobium sangaii SCAU7T (99.9 - 99.6%), Mesorhizobium cantuariense ICMP 19515T (99.7 - 99.6%) and Mesorhizobium ciceri UMP-CA7T (99.7 - 99.5%). Additionally, our strains formed distinct groups in the housekeeping gene analysis and were closely related to Mesorhizobium waimense ICMP 19557T (93...
November 26, 2015: International Journal of Systematic and Evolutionary Microbiology
Dhairyasheel Desai, Jin-Hua Li, Eline van Zijll de Jong, Robert Braun, Andrew Pitman, Sandra Visnovsky, John Hampton, Mary Christey
Xanthomonas campestris pv. campestris is a necrotrophic bacterial pathogen of crucifers. We report here the draft genome sequences of isolates ICMP 4013 and ICMP 21080 from New Zealand. These sequences will facilitate the identification of race-specific factors in X. campestris pv. campestris.
2015: Genome Announcements
Maurizio Brighenti, Andrea Donti, Maria Giulia Gagliardi, Nicola Maschietto, Davide Marini, Maristella Lombardi, Ugo Vairo, Gabriella Agnoletti, Ornella Milanesi, Giacomo Pongiglione, Marco Bonvicini
OBJECTIVES: The objective of this investigation is to evaluate the safety, the impact of endomyocardial biopsy (EMB) results in myocarditis management and the incidence of different etiologies of myocarditis in a pediatric population. BACKGROUND: Although EMB is an established diagnostic tool to evaluate suspected myocarditis, there is lack of clear diagnostic and management guidelines for myocarditis in pediatric patients, particularly in infants. METHODS: We performed a retrospective database review and subsequent outcomes analysis from five Italian pediatric cardiology centers to identify patients aged 0-18 years who underwent EMB for suspected myocarditis or inflammatory cardiomyopathy (ICMP) between 2009 and 2011...
March 2016: Catheterization and Cardiovascular Interventions
Shih-Yi Sheu, Ming-Hui Chen, Wendy Y Y Liu, Mitchell Andrews, Euan K James, Julie K Ardley, Sofie E De Meyer, Trevor K James, John G Howieson, Bruna G Coutinho, Wen-Ming Chen
Seven strains, ICMP 19430T, ICMP 19429, ICMP 19431, WSM4637, WSM4638, WSM4639 and WSM4640, were isolated from nitrogen-fixing nodules on roots of the invasive South African legume Dipogon lignosus (subfamily Papilionoideae, tribe Phaseoleae) in New Zealand and Western Australia, and their taxonomic positions were investigated by using a polyphasic approach. All seven strains grew at 10-37 °C (optimum, 25-30 °C), at pH 4.0-9.0 (optimum, pH 6.0-7.0) and with 0-2 % (w/v) NaCl (optimum growth in the absence of NaCl)...
December 2015: International Journal of Systematic and Evolutionary Microbiology
Matthew D Templeton, Benjamin A Warren, Mark T Andersen, Erik H A Rikkerink, Peter C Fineran
Pseudomonas syringae pv. actinidiae is the causal agent of bacterial canker of kiwifruit, a disease that has rapidly spread worldwide. We have fully sequenced and assembled the chromosomal and plasmid DNA from P. syringae pv. actinidiae ICMP 18884 using the PacBio RS II platform.
2015: Genome Announcements
Karen M Houghton, Xochitl C Morgan, Kirill Lagutin, Andrew D MacKenzie, Mikhail Vyssotskii, Kevin A Mitchell, Ian R McDonald, Hugh W Morgan, Jean F Power, John W Moreau, Eric Hanssen, Matthew B Stott
An aerobic, thermophilic and cellulolytic bacterium, designated strain WKT50.2T, was isolated from geothermal soil at Waikite, New Zealand. Strain WKT50.2T grew at 53-76 °C and at pH 5.9-8.2. The DNA G+C content was 58.4 mol%. The major fatty acids were 12-methyl C18 : 0 and C18 : 0. Polar lipids were all linked to long-chain 1,2-diols, and comprised 2-acylalkyldiol-1-O-phosphoinositol (diolPI), 2-acylalkyldiol-1-O-phosphoacylmannoside (diolP-acylMan), 2-acylalkyldiol-1-O-phosphoinositol acylmannoside (diolPI-acylMan) and 2-acylalkyldiol-1-O-phosphoinositol mannoside (diolPI-Man)...
December 2015: International Journal of Systematic and Evolutionary Microbiology
Sofie E De Meyer, Heng Wee Tan, Peter B Heenan, Mitchell Andrews, Anne Willems
In total 14 strains of Gram-stain-negative, rod-shaped bacteria were isolated from Sophora longicarinata and Sophora microphylla root nodules and authenticated as rhizobia on these hosts. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium, and the strains from S. longicarinata were most closely related to Mesorhizobium amorphae ACCC 19665T (99.8–99.9 %), Mesorhizobium huakuii IAM 14158T (99.8–99.9 %), Mesorhizobium loti USDA 3471T (99.5–99.9 %) and Mesorhizobium septentrionale SDW 014T (99...
October 2015: International Journal of Systematic and Evolutionary Microbiology
Preetinanda Panda, Ashley Lu, Karen F Armstrong, Andrew R Pitman
Pectobacterium species are economically important bacteria that cause soft rotting of potato tubers in the field and in storage. Here, we report the draft genome sequence of the type strain for P. carotovorum subsp. carotovorum, ICMP 5702 (ATCC 15713). The genome sequence of ICMP 5702 will provide an important reference for future phylogenomic and taxonomic studies of the phytopathogenic Enterobacteriaceae.
2015: Genome Announcements
Preetinanda Panda, Mark W E J Fiers, Ashley Lu, Karen F Armstrong, Andrew R Pitman
Blackleg is a disease caused by several species of Pectobacterium that results in losses to potato crops worldwide. Here, we report the draft genomes of three taxonomically and geographically distinct blackleg-causing strains of Pectobacterium: P. carotovorum subsp. brasiliensis ICMP 19477, P. atrosepticum ICMP 1526, and P. carotovorum subsp. carotovorum UGC32. Comparison of these genomes will support the identification of common traits associated with their capacity to cause blackleg.
2015: Genome Announcements
A Cunty, S Cesbron, F Poliakoff, M-A Jacques, C Manceau
The first outbreaks of bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae biovar 3 were detected in France in 2010. P. syringae pv. actinidiae causes leaf spots, dieback, and canker that sometimes lead to the death of the vine. P. syringae pv. actinidifoliorum, which is pathogenic on kiwi as well, causes only leaf spots. In order to conduct an epidemiological study to track the spread of the epidemics of these two pathogens in France, we developed a multilocus variable-number tandem-repeat (VNTR) analysis (MLVA)...
October 2015: Applied and Environmental Microbiology
Giulia Ottaviani, Rajko Radovancevic, Biswajit Kar, Igor Gregoric, L Maximilian Buja
BACKGROUND: To date, there has been little research, if any, on the pathological correlates of end-stage heart failure in the explanted hearts of orthotopic heart transplant (OHT) recipients in correlation with the patients' hemodynamics. We sought to compare the gross and histopathological parameters in hearts explanted-native or previously transplanted-from patients with end-stage heart failure with the clinical hemodynamics parameters at the time of OHT. METHODS: Forty patients undergoing OHT were enrolled in this study and divided into two groups according to whether they suffered from ischemic (ICMP) or nonischemic cardiomyopathy (NICMP)...
September 2015: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
Mischa T Rijnierse, Cornelis P Allaart, Stefan de Haan, Hendrik J Harms, Marc C Huisman, LiNa Wu, Aernout M Beek, Adriaan A Lammertsma, Albert C van Rossum, Paul Knaapen
AIMS: Sympathetic denervation typically occurs in the infarcted myocardium and is associated with sudden cardiac death. Impaired innervation was also demonstrated in non-infarcted myocardium in ischaemic and dilated cardiomyopathy (ICMP and DCMP). Factors affecting sympathetic nerve integrity in remote myocardium are unknown. Perfusion abnormalities, even in the absence of epicardial coronary artery disease, may relate to sympathetic dysfunction. This study was aimed to assess the interrelations of myocardial blood flow (MBF), contractile function, and sympathetic innervation in non-infarcted remote myocardium...
July 2015: European Heart Journal Cardiovascular Imaging
Heike Anders, Jean F Power, Andrew D MacKenzie, Kirill Lagutin, Mikhail Vyssotski, Eric Hanssen, John W Moreau, Matthew B Stott
A novel bacterial strain, NGM72.4(T), was isolated from a hot spring in the Ngatamariki geothermal field, New Zealand. Phylogenetic analysis based on 16S rRNA gene sequences grouped it into the phylum Verrucomicrobia and class level group 3 (also known as OPB35 soil group). NGM72.4(T) stained Gram-negative, and was catalase- and oxidase-positive. Cells were small cocci, 0.5-0.8 µm in diameter, which were motile by means of single flagella. Transmission electron micrograph (TEM) imaging showed an unusual pirellulosome-like intracytoplasmic membrane...
April 2015: International Journal of Systematic and Evolutionary Microbiology
S Sasso, L Scrano, M G Bonomo, G Salzano, S A Bufo
Biological sciences and related bio-technology play a very important role in research projects concerning protection and preservation of cultural heritage for future generations. In this work secondary metabolites of Burkholderia gladioli pv. agaricicola (Bga) ICMP 11096 strain and crude extract of glycoalkaloids from Solanaceae plants, were tested against a panel of microorganisms isolated from calcarenite stones of two historical bridges located in Potenza and in Campomaggiore (Southern Italy). The isolated bacteria belong to Bacillus cereus and Arthrobacter agilis species, while fungi belong to Aspergillus, Penicillium, Coprinellus, Fusarium, Rhizoctonio and Stemphylium genera...
2013: Communications in Agricultural and Applied Biological Sciences
Farhan M Said, John Brooks, Yusuf Chisti
The carbon-to-nitrogen (C:N) ratio in the biomass of microfungi tends to be quite different (e.g. 10-15) compared with the C:N ratio in the red pigments (e.g. >20) of the fungus Monascus ruber. Therefore, determining an optimal C:N ratio in the culture medium for maximizing the production of the pigments is important. A culture medium composition is established for maximizing the production of the red pigment by the fungus M. ruber ICMP 15220 in submerged culture. The highest volumetric productivity of the red pigment was 0...
September 2014: World Journal of Microbiology & Biotechnology
C-Thomas Bock, Anja Düchting, Friederike Utta, Eva Brunner, Bui Tien Sy, Karin Klingel, Florian Lang, Meinrad Gawaz, Stephan B Felix, Reinhard Kandolf
AIM: To investigate molecular phenotypes of myocardial B19V-infection to determine the role of B19V in myocarditis and dilated cardiomyopathy (DCM). METHODS: Endomyocardial biopsies (EMBs) from 498 B19V-positive patients with myocarditis and DCM were analyzed using molecular methods and functional experiments. EMBs were obtained from the University Hospitals of Greifswald and Tuebingen and additionally from 36 German cardiology centers. Control tissues were obtained at autopsy from 34 victims of accidents, crime or suicide...
April 26, 2014: World Journal of Cardiology
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