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Sandra B Visnovsky, Preetinanda Panda, Robert Taylor, Andrew R Pitman
Pectobacterium carotovorum subsp. actinidiae is the causal agent of summer canker in kiwifruit plants in South Korea. We report here the draft genome sequences of two P. carotovorum subsp. actinidiae strains, ICMP 19971 and ICMP 19972, which were originally isolated from Actinidia chinensis with symptoms of summer canker. These genome sequences will aid in the identification of genetic traits associated with their unusual capacity to cause canker and help understanding of the threat these exotic enterobacteria pose to the New Zealand kiwifruit industry...
April 6, 2017: Genome Announcements
Hiroaki Hayashi, Keisuke Usui, Yuji Nakaguchi, Yuki Kanazawa, Takahiro Shimo, Yuki Matsumoto
No abstract text is available yet for this article.
2017: Nihon Hoshasen Gijutsu Gakkai Zasshi
Munusamy Madhaiyan, Selvaraj Poonguzhali, Venkatakrishnan Sivaraj Saravanan, Kumaran Selvapravin, Veeramuthu Duraipandiyan, Naif Abdullah Al-Dhabi
A novel Gram-stain negative, aerobic, motile, rod-shaped bacterium was isolated from Sesame (Sesamum indicum L.) rhizosphere soil. Based on the 16S rRNA gene similarity value (99.4-98.6%) obtained with phylogenetically closely related strains and through analyses of their house keeping genes (atpD, infB and rpoB), the strain SI-P133(T) was delineated among the species of the genus Pseudomonas and was subjected to polyphasic taxonomic analysis. It was a chemoorganotroph which grew at wide range of temperature (4-45 °C), pH (5...
March 20, 2017: Antonie Van Leeuwenhoek
Christian Besler, David Lang, Daniel Urban, Karl-Philipp Rommel, Maximilian von Roeder, Karl Fengler, Stephan Blazek, Reinhard Kandolf, Karin Klingel, Holger Thiele, Axel Linke, Gerhard Schuler, Volker Adams, Philipp Lurz
BACKGROUND: Galectin (Gal)-3 is a β-galactoside-binding lectin and currently intensely studied as a biomarker in heart failure. Gal-3 also exerts proinflammatory effects, at least in extracardiac tissues. Objective of this study was to characterize the relationship of plasma and myocardial Gal-3 levels with cardiac fibrosis and inflammation in patients with nonischemic dilated cardiomyopathy and inflammatory cardiomyopathy (iCMP). METHODS AND RESULTS: Endomyocardial biopsies and blood samples were obtained from patients with newly diagnosed cardiomyopathy and clinical suspicion of myocarditis...
March 2017: Circulation. Heart Failure
Carl H Mesarich, Jonathan Rees-George, Paul P Gardner, Fatemeh Ashari Ghomi, Monica L Gerth, Mark T Andersen, Erik H A Rikkerink, Peter C Fineran, Matthew D Templeton
Pseudomonas syringae pv. actinidiae (Psa), the causal agent of kiwifruit canker, is one of the most devastating plant diseases of recent times. We have generated two mini-Tn5-based random insertion libraries of Psa ICMP 18884. The first, a 'phenotype of interest' (POI) library, consists of 10,368 independent mutants gridded into 96-well plates. By replica plating onto selective media, the POI library was successfully screened for auxotrophic and motility mutants. Lipopolysaccharide (LPS) biosynthesis mutants with 'Fuzzy-Spreader'-like morphologies were also identified through a visual screen...
2017: PloS One
Hazem S Elshafie, Rocco Racioppi, Sabino A Bufo, Ippolito Camele
This research was carried out to study in vitro antibacterial activity of 4 strains of Burkholderia gladioli pv. agaricicola (Bga) against G+ve Bacillus megaterium and G-ve Escherichia coli, haemolytic activity against the cell membrane of erythrocytes, the production of extracellular hydrolytic enzymes and finally, the pathogenicity against Agaricus bisporus flesh blocks. Chemical structure of bioactive substances of the most bioactive strain (ICMP 11096) was established using gas chromatography-mass spectrometry (GC-MS)...
February 2017: Saudi Journal of Biological Sciences
Matthias Mueller-Hennessen, Hans-Dirk Düngen, Matthias Lutz, Tobias Daniel Trippel, Michael Kreuter, Johanna Sigl, Oliver J Müller, Elvis Tahirovic, Henning Witt, Philipp Ternes, Susan Carvalho, Erik Peter, Dietrich Rein, Philipp Schatz, Felix Herth, Evangelos Giannitsis, Tanja Weis, Norbert Frey, Hugo A Katus
OBJECTIVES: In this study we aimed to identify novel metabolomic biomarkers suitable for improved diagnosis of heart failure with reduced ejection fraction (HFrEF). METHODS: We prospectively recruited 887 individuals consisting of HFrEF patients with either ischemic (ICMP, n = 257) or nonischemic cardiomyopathy (NICMP, n = 269), healthy controls (n = 327), and patients with pulmonary diseases (n = 34). A single-center identification (n = 238) was followed by a multicenter confirmation study (n = 649)...
January 2017: Clinical Chemistry
Munusamy Madhaiyan, Selvaraj Poonguzhali, Venkatakrishnan Sivaraj Saravanan, Veeramuthu Duraipandiyan, Naif Abdullah Al-Dhabi, Dhandapani Pragatheswari, Palani Santhanakrishnan, Soo-Jin Kim, Hang-Yeon Weon, Soon-Wo Kwon
A novel siderophore-producing actinomycete, designated PL19T, was isolated from the Scots-pine needle-like leaves collected from TNAU campus, Coimbatore, India. The isolate was chemoorganotrophic in nutrition and able to grow at 30 °C, and the optimum pH and NaCl facilitated the growth pH 6-11 and 0-8 % (w/v), respectively. The cells are filamentous and the mycelia formed are basically of wide and intricately branched substrate mycelium from which aerial mycelia arises, later gets differentiated into spores that are warty and arranged spirally...
October 2016: International Journal of Systematic and Evolutionary Microbiology
Christian Besler, Daniel Urban, Stefan Watzka, David Lang, Karl-Philipp Rommel, Reinhard Kandolf, Karin Klingel, Holger Thiele, Axel Linke, Gerhard Schuler, Volker Adams, Philipp Lurz
AIMS: Inflammatory heart disease represents an important cause of chronic dilated cardiomyopathy (DCM). Predicting the clinical course of patients with inflammatory cardiomyopathy (iCMP) is difficult, and the prognostic value of current biological markers remains controversial. We tested whether expression of selected microRNAs in endomyocardial biopsies (EMBs) is related to LV functional recovery and clinical events in iCMP patients. METHODS AND RESULTS: EMBs were obtained from patients with iCMP (n = 76) and non-inflammatory DCM (n = 22)...
December 2016: European Journal of Heart Failure
Dieter Dauwe, Beatriz Pelacho, Arief Wibowo, Ann-Sophie Walravens, Kristoff Verdonck, Hilde Gillijns, Ellen Caluwe, Peter Pokreisz, Nick van Gastel, Geert Carmeliet, Maarten Depypere, Frederik Maes, Nina Vanden Driessche, Walter Droogne, Johan Van Cleemput, Johan Vanhaecke, Felipe Prosper, Catherine Verfaillie, Aernout Luttun, Stefan Janssens
BACKGROUND: Blood outgrowth endothelial cells (BOECs) mediate therapeutic neovascularization in experimental models, but outgrowth characteristics and functionality of BOECs from patients with ischemic cardiomyopathy (ICMP) are unknown. We compared outgrowth efficiency and in vitro and in vivo functionality of BOECs derived from ICMP with BOECs from age-matched (ACON) and healthy young (CON) controls. METHODS AND RESULTS: We isolated 3.6±0.6 BOEC colonies/100×10(6) mononuclear cells (MNCs) from 60-mL blood samples of ICMP patients (n=45; age: 66±1 years; LVEF: 31±2%) versus 3...
April 18, 2016: Journal of the American Heart Association
Ben Corteville, Jan De Pooter, Tine De Backer, Milad El Haddad, Roland Stroobandt, Frank Timmermans
AIMS: In patients with systolic heart failure and left bundle branch block (LBBB), septal flash (SF) movement has been described by echocardiography. We evaluated the prevalence of SF in LBBB and non-LBBB patients and evaluated whether specific electrocardiographic (ECG) characteristics within LBBB are associated with the presence of SF on echocardiography. METHODS AND RESULTS: One hundred and four patients with probable LBBB on standard 12-lead ECG were selected, 40 patients with non-LBBB served as controls...
January 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
Sofie E De Meyer, Heng Wee Tan, Mitchell Andrews, Peter B Heenan, Anne Willems
In total, 31 strains of gram-negative, rod-shaped bacteria were isolated from Sophora root nodules and authenticated as rhizobia on this hos. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium, with the representative strains ICMP 19560T, ICMP 19523T, ICMP 19535T, ICMP 19545T and ICMP 19512T being most closely related to Mesorhizobium sangaii SCAU7T (99.9 - 99.6%), Mesorhizobium cantuariense ICMP 19515T (99.7 - 99.6%) and Mesorhizobium ciceri UMP-CA7T (99.7 - 99.5%). Additionally, our strains formed distinct groups in the housekeeping gene analysis and were closely related to Mesorhizobium waimense ICMP 19557T (93...
November 26, 2015: International Journal of Systematic and Evolutionary Microbiology
Dhairyasheel Desai, Jin-Hua Li, Eline van Zijll de Jong, Robert Braun, Andrew Pitman, Sandra Visnovsky, John Hampton, Mary Christey
Xanthomonas campestris pv. campestris is a necrotrophic bacterial pathogen of crucifers. We report here the draft genome sequences of isolates ICMP 4013 and ICMP 21080 from New Zealand. These sequences will facilitate the identification of race-specific factors in X. campestris pv. campestris.
2015: Genome Announcements
Maurizio Brighenti, Andrea Donti, Maria Giulia Gagliardi, Nicola Maschietto, Davide Marini, Maristella Lombardi, Ugo Vairo, Gabriella Agnoletti, Ornella Milanesi, Giacomo Pongiglione, Marco Bonvicini
OBJECTIVES: The objective of this investigation is to evaluate the safety, the impact of endomyocardial biopsy (EMB) results in myocarditis management and the incidence of different etiologies of myocarditis in a pediatric population. BACKGROUND: Although EMB is an established diagnostic tool to evaluate suspected myocarditis, there is lack of clear diagnostic and management guidelines for myocarditis in pediatric patients, particularly in infants. METHODS: We performed a retrospective database review and subsequent outcomes analysis from five Italian pediatric cardiology centers to identify patients aged 0-18 years who underwent EMB for suspected myocarditis or inflammatory cardiomyopathy (ICMP) between 2009 and 2011...
March 2016: Catheterization and Cardiovascular Interventions
Shih-Yi Sheu, Ming-Hui Chen, Wendy Y Y Liu, Mitchell Andrews, Euan K James, Julie K Ardley, Sofie E De Meyer, Trevor K James, John G Howieson, Bruna G Coutinho, Wen-Ming Chen
Seven strains, ICMP 19430T, ICMP 19429, ICMP 19431, WSM4637, WSM4638, WSM4639 and WSM4640, were isolated from nitrogen-fixing nodules on roots of the invasive South African legume Dipogon lignosus (subfamily Papilionoideae, tribe Phaseoleae) in New Zealand and Western Australia, and their taxonomic positions were investigated by using a polyphasic approach. All seven strains grew at 10-37 °C (optimum, 25-30 °C), at pH 4.0-9.0 (optimum, pH 6.0-7.0) and with 0-2 % (w/v) NaCl (optimum growth in the absence of NaCl)...
December 2015: International Journal of Systematic and Evolutionary Microbiology
Matthew D Templeton, Benjamin A Warren, Mark T Andersen, Erik H A Rikkerink, Peter C Fineran
Pseudomonas syringae pv. actinidiae is the causal agent of bacterial canker of kiwifruit, a disease that has rapidly spread worldwide. We have fully sequenced and assembled the chromosomal and plasmid DNA from P. syringae pv. actinidiae ICMP 18884 using the PacBio RS II platform.
2015: Genome Announcements
Karen M Houghton, Xochitl C Morgan, Kirill Lagutin, Andrew D MacKenzie, Mikhail Vyssotskii, Kevin A Mitchell, Ian R McDonald, Hugh W Morgan, Jean F Power, John W Moreau, Eric Hanssen, Matthew B Stott
An aerobic, thermophilic and cellulolytic bacterium, designated strain WKT50.2T, was isolated from geothermal soil at Waikite, New Zealand. Strain WKT50.2T grew at 53-76 °C and at pH 5.9-8.2. The DNA G+C content was 58.4 mol%. The major fatty acids were 12-methyl C18 : 0 and C18 : 0. Polar lipids were all linked to long-chain 1,2-diols, and comprised 2-acylalkyldiol-1-O-phosphoinositol (diolPI), 2-acylalkyldiol-1-O-phosphoacylmannoside (diolP-acylMan), 2-acylalkyldiol-1-O-phosphoinositol acylmannoside (diolPI-acylMan) and 2-acylalkyldiol-1-O-phosphoinositol mannoside (diolPI-Man)...
December 2015: International Journal of Systematic and Evolutionary Microbiology
Sofie E De Meyer, Heng Wee Tan, Peter B Heenan, Mitchell Andrews, Anne Willems
In total 14 strains of Gram-stain-negative, rod-shaped bacteria were isolated from Sophora longicarinata and Sophora microphylla root nodules and authenticated as rhizobia on these hosts. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Mesorhizobium, and the strains from S. longicarinata were most closely related to Mesorhizobium amorphae ACCC 19665T (99.8–99.9 %), Mesorhizobium huakuii IAM 14158T (99.8–99.9 %), Mesorhizobium loti USDA 3471T (99.5–99.9 %) and Mesorhizobium septentrionale SDW 014T (99...
October 2015: International Journal of Systematic and Evolutionary Microbiology
Preetinanda Panda, Ashley Lu, Karen F Armstrong, Andrew R Pitman
Pectobacterium species are economically important bacteria that cause soft rotting of potato tubers in the field and in storage. Here, we report the draft genome sequence of the type strain for P. carotovorum subsp. carotovorum, ICMP 5702 (ATCC 15713). The genome sequence of ICMP 5702 will provide an important reference for future phylogenomic and taxonomic studies of the phytopathogenic Enterobacteriaceae.
2015: Genome Announcements
Preetinanda Panda, Mark W E J Fiers, Ashley Lu, Karen F Armstrong, Andrew R Pitman
Blackleg is a disease caused by several species of Pectobacterium that results in losses to potato crops worldwide. Here, we report the draft genomes of three taxonomically and geographically distinct blackleg-causing strains of Pectobacterium: P. carotovorum subsp. brasiliensis ICMP 19477, P. atrosepticum ICMP 1526, and P. carotovorum subsp. carotovorum UGC32. Comparison of these genomes will support the identification of common traits associated with their capacity to cause blackleg.
2015: Genome Announcements
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