keyword
https://read.qxmd.com/read/38464014/survivin-mediates-mitotic-onset-in-hela-cells-through-activation-of-the-cdk1-cdc25b-axis
#21
Pedro M Cánovas
The Survivin protein has roles in repairing incorrect microtubule-kinetochore attachments at prometaphase, and the faithful execution of cytokinesis, both as part of the chromosomal passenger complex (CPC) (1). In this context, errors frequently lead to aneuploidy, polyploidy and cancer (1). Adding to these well-known roles of this protein, this paper now shows for the first time that Survivin is required for cancer cells to enter mitosis, and that, in its absence, HeLa cells accumulate at early prophase, or prior to reported before (2, 3)...
February 28, 2024: Research Square
https://read.qxmd.com/read/38463586/expression-of-circular-rna-has-circ-0009158-and-identification-of-associated-mirna-mrna-network-in-hepatocellular-carcinoma
#22
JOURNAL ARTICLE
Lili Sun, Buerlan Yeerkenbieke, Anwaier Yuemaierabola, Fuzhong Liu, Dilinaer Yeerxiati, Xiaogang Dong, Wenjia Guo
Primary hepatocellular carcinoma (HCC) affects people all over the world. Circular RNAs are involved in the growth and development of several malignancies and regulate a number of biological processes. However, the roles of has-circ-0009158 in HCC remain unknown. This study explored the expression and associated miRNA-mRNA network of has-circ-0009158 in HCC. Quantitative real-time polymerase chain reaction was used to measure the expression of hsa-circ-0009158 in the HCC tissues of 143 patients and four human HCC cell lines...
2024: American Journal of Translational Research
https://read.qxmd.com/read/38462164/o-glcnacylation-stimulates-the-deubiquitination-activity-of-usp16-and-regulates-cell-cycle-progression
#23
JOURNAL ARTICLE
Jianxin Zhao, Jie Hua, Yahui Zhan, Chunxu Chen, Yue Liu, Liqian Yang, Haiying Wang, Hengbin Wang, Jing Li
Histone 2A monoubiquitination (uH2A) underscores a key epigenetic regulation of gene expression. In this report, we show that the deubiquitinase (DUB) for uH2A, Ubiquitin Specific Peptidase 16 (USP16), is modified by O-linked N-acetylglucosamine (O-GlcNAc). O-GlcNAcylation involves the installation of the O-GlcNAc moiety to Ser/Thr residues. It crosstalks with Ser/Thr phosphorylation, affects protein-protein interaction, alters enzyme activity or protein folding, and changes protein subcellular localization...
March 8, 2024: Journal of Biological Chemistry
https://read.qxmd.com/read/38456590/cdk1-promotes-the-phosphorylation-of-kifc1-to-regulate-the-tumorgenicity-of-endometrial-carcinoma
#24
JOURNAL ARTICLE
Xi Lin, Yingying He, Yiming Liu, Huihao Zhou, Xiaomin Xu, Jingui Xu, Kening Zhou
OBJECTIVE: This study aims to clarify the mechanical action of cyclin-dependent protein kinase 1 (CDK1) in the development of endometrial carcinoma (EMCA), which may be associated with the phosphorylation of kinesin family member C1 (KIFC1) and further activate the PI3K/AKT pathway. METHODS: The protein and gene expression of CDK1 in EMCA tissues and tumor cell lines were evaluated by western blot, quantitative polymerase chain reaction, and immunohistochemistry staining...
February 26, 2024: Journal of Gynecologic Oncology
https://read.qxmd.com/read/38456502/cdk1-inhibition-reduces-osteogenesis-in-endothelial-cells-in-vascular-calcification
#25
JOURNAL ARTICLE
Yan Zhao, Yang Yang, Xiuju Wu, Li Zhang, Xinjiang Cai, Jaden Ji, Sydney Chen, Abigail Vera, Kristina I Boström, Yucheng Yao
Vascular calcification is a severe complication of cardiovascular diseases. Previous studies demonstrated that endothelial lineage cells transitioned into osteoblast-like cells and contributed to vascular calcification. Here, we found that inhibition of cyclin-dependent kinase (CDK) prevented endothelial lineage cells from transitioning to osteoblast-like cells and reduced vascular calcification. We identified a robust induction of CDK1 in endothelial cells (ECs) in calcified arteries and showed that EC-specific gene deletion of CDK1 decreased the calcification...
March 8, 2024: JCI Insight
https://read.qxmd.com/read/38453961/comprehensive-multi-omics-analysis-reveals-wee1-as-a-synergistic-lethal-target-with-hyperthermia-through-cdk1-super-activation
#26
JOURNAL ARTICLE
Xiaohang Yang, Xingyuan Hu, Jingjing Yin, Wenting Li, Yu Fu, Bin Yang, Junpeng Fan, Funian Lu, Tianyu Qin, Xiaoyan Kang, Xucui Zhuang, Fuxia Li, Rourou Xiao, Tingyan Shi, Kun Song, Jing Li, Gang Chen, Chaoyang Sun
Hyperthermic intraperitoneal chemotherapy's role in ovarian cancer remains controversial, hindered by limited understanding of hyperthermia-induced tumor cellular changes. This limits developing potent combinatory strategies anchored in hyperthermic intraperitoneal therapy (HIPET). Here, we perform a comprehensive multi-omics study on ovarian cancer cells under hyperthermia, unveiling a distinct molecular panorama, primarily characterized by rapid protein phosphorylation changes. Based on the phospho-signature, we pinpoint CDK1 kinase is hyperactivated during hyperthermia, influencing the global signaling landscape...
March 7, 2024: Nature Communications
https://read.qxmd.com/read/38452691/telocinobufagin-a-plk1-suppressor-that-inhibits-tumor-growth-and-metastasis-by-modulating-cdc25c-and-ctcf-in-hnscc-cells
#27
JOURNAL ARTICLE
Jie Li, Ru Ma, Jun-Lin Lv, Yu-Shan Ren, Yu-Jun Tan, Hao-Mai Wang, Zhui-En Wang, Bin-Sheng Wang, Jia-Ning Yu, Yu-Liang Wang, Jun Tian, Qiu-Sheng Zheng
BACKGROUND: The high metastasis and mortality rates of head and neck squamous cell carcinoma (HNSCC) urgently require new treatment targets and drugs. A steroidal component of ChanSu, telocinobufagin (TBG), was verified to have anti-cancer effects in various tumors, but its activity and mechanism in anti-HNSCC were still unknown. PURPOSE: This study tried to demonstrate the anti-tumor effect of TBG on HNSCC and verify its potential mechanism. METHODS: The effect of TBG on cell proliferation and metastasis were performed and the TBG changed genes were detected by RNA-seq analysis in HNSCC cells...
February 9, 2024: Phytomedicine
https://read.qxmd.com/read/38447274/differential-anti-viral-response-to-respiratory-syncytial-virus-a-in-preterm-and-term-infants
#28
JOURNAL ARTICLE
Jeremy Anderson, Samira Imran, Yan Yung Ng, Tongtong Wang, Sarah Ashley, Cao Minh Thang, Le Quang Thanh, Vo Thi Trang Dai, Phan Van Thanh, Bui Thi Hong Nhu, Do Ngoc Xuan Trang, Phan Thi Phuong Trinh, Le Thanh Binh, Nguyen Thuong Vu, Nguyen Trong Toan, Boris Novakovic, Mimi L K Tang, Danielle Wurzel, Kim Mulholland, Daniel G Pellicci, Lien Anh Ha Do, Paul V Licciardi
BACKGROUND: Preterm infants are more likely to experience severe respiratory syncytial virus (RSV) disease compared to term infants. The reasons for this are multi-factorial, however their immature immune system is believed to be a major contributing factor. METHODS: We collected cord blood from 25 preterm (gestational age 30.4-34.1 weeks) and 25 term infants (gestation age 37-40 weeks) and compared the response of cord blood mononuclear cells (CBMCs) to RSVA and RSVB stimulation using neutralising assays, high-dimensional flow cytometry, multiplex cytokine assays and RNA-sequencing...
March 5, 2024: EBioMedicine
https://read.qxmd.com/read/38446308/kinesin-family-member-18a-kif18a-promotes-cell-proliferation-and-metastasis-in-hepatocellular-carcinoma
#29
JOURNAL ARTICLE
Jihua Ren, Xinyan Yao, Minli Yang, Shengtao Cheng, Daiqing Wu, Kexin Xu, Ranran Li, Han Zhang, Dapeng Zhang
BACKGROUND & AIMS: Kinesin family member 18A (KIF18A) is notable for its aberrant expression across various cancer types and its pivotal role is driving cancer progression. In this study, we aim to investigate the intricate molecular mechanisms underlying the impact of KIF18A on the progression of HCC. METHODS: Western blotting assays, a quantitative real-time PCR and immunohistochemical analyses were performed to quantitatively assess KIF18A expression in HCC tissues...
March 6, 2024: Digestive Diseases and Sciences
https://read.qxmd.com/read/38434984/microrna-485-5p-targets-keratin17-to-regulate-pancreatic-cancer-cell-proliferation-and-invasion-via-the-fak-src-erk-pathway
#30
JOURNAL ARTICLE
Peng Chen, Meng Pan, Zhengchao Shen, Yuquan Yang, Xiaoming Wang
Background: It is crucial to probe into the biological effect and mechanism of miRNA-485-5p regulating keratin 17 (KRT17) in pancreatic cancer (PC) to understand its pathogenesis and identify potential biological targets. Methods: The bioinformatics means were used to evaluate the clinical significance of KRT17 expression in the Cancer Genome Atlas (TCGA) database. TargetScan database analysis in conjunction with dual luciferase and RNA Immunoprecipitation (RIP) experiments was used to probe the interaction relationship of miRNA-485-5p with KRT17...
2024: Journal of Cancer
https://read.qxmd.com/read/38434406/molecular-and-functional-anticancer-effects-of-glp-g9a-inhibition-by-unc0646-in-mewo-melanoma-cells
#31
JOURNAL ARTICLE
Luma Dayane de Carvalho Filiú-Braga, Amanda Évelin Silva-Carvalho, Marielly Reis Resende Sousa, Juliana Lott Carvalho, Felipe Saldanha-Araujo
In recent years, histone methyltransferases (HMTs) have emerged as important therapeutic targets in cancer due to their oncogenic role. Herein, we used the GLP/G9a inhibitor UNC0646 to assess whether the inhibition of such HMTs could induce cell death in MeWo melanoma cells. Furthermore, we investigated the cellular and molecular mechanisms involved in the observed cell death events. Finally, we performed a functional genomics analysis of 480 melanoma samples to characterize G9a/GLP involvement in melanoma...
March 15, 2024: Heliyon
https://read.qxmd.com/read/38429268/otud4-promotes-the-progression-of-glioblastoma-by-deubiquitinating-cdk1-and-activating-mapk-signaling-pathway
#32
JOURNAL ARTICLE
Mingxin Ci, Gaichao Zhao, Chongyang Li, Ruochen Liu, Xiaosong Hu, Jun Pan, Yang Shen, Guanghui Zhang, Yongsen Li, Li Zhang, Ping Liang, Hongjuan Cui
Glioblastoma, IDH-Wild type (GBM, CNS WHO Grade 4) is a highly heterogeneous and aggressive primary malignant brain tumor with high morbidity, high mortality, and poor patient prognosis. The global burden of GBM is increasing notably due to limited treatment options, drug delivery problems, and the lack of characteristic molecular targets. OTU deubiquitinase 4 (OTUD4) is a potential predictive factor for several cancers such as breast cancer, liver cancer, and lung cancer. However, its function in GBM remains unknown...
March 1, 2024: Cell Death & Disease
https://read.qxmd.com/read/38422700/discovery-of-novel-urea-derivatives-as-ferroptosis-and-autophagy-inducer-for-human-colon-cancer-treatment
#33
JOURNAL ARTICLE
Tingting Liang, Haiyang Dong, Zhuangzhuang Wang, Lu Lu, Xueting Song, Jianguo Qi, Yahong Zhang, Jianhong Wang, Guanhua Du
A series of novel urea derivatives were designed, synthesized and evaluated for their inhibitory activities against HT-29 cells, and structure-activity relationships (SAR) were summarized. Compound 10p stood out from these derivatives, exhibiting the most potent antiproliferative activity. Further biological studies demonstrated that 10p arrested cell cycle at G2/M phase via regulating cell cycle-related proteins CDK1 and Cyclin B1. The underlying molecular mechanisms demonstrated that 10p induced cell death through ferroptosis and autophagy, but not apoptosis...
February 24, 2024: European Journal of Medicinal Chemistry
https://read.qxmd.com/read/38420905/comprehensive-parameter-space-mapping-of-cell-cycle-dynamics-under-network-perturbations
#34
JOURNAL ARTICLE
Zhengda Li, Shiyuan Wang, Meng Sun, Minjun Jin, Daniel Khain, Qiong Yang
Studies of quantitative systems and synthetic biology have extensively utilized models to interpret data, make predictions, and guide experimental designs. However, models often simplify complex biological systems and lack experimentally validated parameters, making their reliability in perturbed systems unclear. Here, we developed a droplet-based synthetic cell system to continuously tune parameters at the single-cell level in multiple dimensions with full dynamic ranges, providing an experimental framework for global parameter space scans...
February 29, 2024: ACS Synthetic Biology
https://read.qxmd.com/read/38415071/the-cdc14-phosphatase-clp1-does-not-affect-genome-expression
#35
JOURNAL ARTICLE
Luis Lopez Maury, Liping Ren, Shaimaa Hassan, Jürg Bähler, Kathleen L Gould
Schizosaccharomyces pombe Clp1 is a Cdc14-family phosphatase that reverses mitotic Cdk1 phosphorylation. Despite evolutionary conservation, Clp1 's mammalian orthologs do not share this function. Rather, higher eukaryotic Cdc14 enzymes act in DNA repair, ciliogenesis, and gene regulation. To examine if Clp1 regulates gene expression, we compared the transcriptional profiles of cells lacking Clp1 function to that of wildtype. Because clp1∆ cells are sensitive to the actin depolymerizing drug, LatrunculinA, we also investigated whether a transcriptional response was involved...
2024: microPublication. Biology
https://read.qxmd.com/read/38413888/rna-sequencing-identifies-key-genes-involved-in-intramuscular-fat-deposition-in-chickens-at-different-developmental-stages
#36
JOURNAL ARTICLE
Jinmei Zhu, Yongli Wang, Yongchun Su, Maiqing Zheng, Huanxian Cui, Zhiwu Chen
BACKGROUND: Intramuscular fat (IMF) is an important factor in meat quality, and triglyceride (TG) and Phospholipids (PLIP), as the main components of IMF, are of great significance to the improvement of meat quality. RESULTS: In this study, we used 30 RNA sequences generated from the transcriptome of chicken breast muscle tissues at different developmental stages to construct a gene expression matrix to map RNA sequence reads to the chicken genome and identify the transcript of origin...
February 27, 2024: BMC Genomics
https://read.qxmd.com/read/38412667/cucurbitacin-b-suppresses-hepatocellular-carcinoma-progression-through-inducing-dna-damage-dependent-cell-cycle-arrest
#37
JOURNAL ARTICLE
Qi-Zhang Li, Yu-Ying Chen, Qiu-Ping Liu, Zhi-Hui Feng, Lei Zhang, Hong Zhang
BACKGROUND: The mortality rate of liver cancer ranks third in the world, and hepatocellular carcinoma (HCC) is a malignant tumor of the digestive tract. Cucurbitacin B (CuB), a natural compound extracted from Cucurbitaceae spp., is the main active component of Chinese patent medicine the Cucurbitacin Tablet, which has been widely used in the treatment of various malignant tumors in clinics, especially HCC. PURPOSE: This study explored the role and mechanism of CuB in the suppression of liver cancer progression...
April 2024: Phytomedicine
https://read.qxmd.com/read/38412650/new-2-oxoindole-derivatives-as-multiple-pdgfr%C3%AE-%C3%A3-and-vegfr-2-tyrosine-kinase-inhibitors
#38
JOURNAL ARTICLE
Hend A A Ezelarab, Amer Ali Abd El-Hafeez, Taha F S Ali, Ahmed M Sayed, Heba A Hassan, Eman A M Beshr, Samar H Abbas
Two new series of N-aryl acetamides 6a-o and benzyloxy benzylidenes 9a-p based 2-oxoindole derivatives were designed as potent antiproliferative multiple kinase inhibitors. The results of one-dose NCI antiproliferative screening for compounds 6a-o and 9a-p elucidated that the most promising antiproliferative scaffolds were 6f and 9f, which underwent five-dose testing. Notably, the amido congener 6f was the most potent derivative towards pancreatic ductal adenocarcinoma MDA-PATC53 and PL45 cell lines (IC50  = 1...
February 22, 2024: Bioorganic Chemistry
https://read.qxmd.com/read/38410612/high-resolution-transcriptomics-analysis-of-cxcl13-epsti1-cdk1-cells-with-a-specific-focus-on-lung-adenocarcinoma
#39
JOURNAL ARTICLE
Longjin Zeng, Xu Chen, Jianxiong Cui, Longyao Zhang, Lingchen Li, Chenrui Yin, Xiewan Chen, Jianguo Sun
BACKGROUND: Programmed cell death ligand 1 (PD-L1) blocking therapy has transformed the treatment of lung adenocarcinoma (LUAD), which has significantly changed the landscape of immunotherapy. We aimed to explore specific cell subpopulations to understand tumor progression and identify markers of response to PD-L1 blocking therapy. METHODS: Bulk, fluorescence-activated cell sorting (FACS), and single-cell RNA (scRNA) sequencing were used to profile CXCL13 , EPSTI1 , and CDK1 ...
January 30, 2024: Journal of Thoracic Disease
https://read.qxmd.com/read/38410486/targeting-ccne1-amplified-ovarian-and-endometrial-cancers-by-combined-inhibition-of-pkmyt1-and-atr
#40
Fiona Simpkins, Haineng Xu, Erin Geroge, David Gallo, Sergey Medvedev, Xiaolei Wang, Rosie Kryczka, Marc Hyer, Jimmy Fourtounis, Rino Stocco, Elia Aguado-Fraile, Adam Petrone, Shou Yun Yin, Ariya Shiwram, Matthew Anderson, Hyoung Kim, Fang Liu, Gary Marshall
Ovarian cancers (OVCAs) and endometrial cancers (EMCAs) with CCNE1-amplification are often resistant to standard of care treatment and represent an unmet clinical need. Previously, synthetic-lethal screening identified loss of the CDK1 regulator, PKMYT1, as synthetically lethal with CCNE1-amplification. We hypothesized that CCNE1-amplification associated replication stress will be more effectively targeted by combining the PKMYT1 inhibitor, lunresertib (RP-6306), with the ATR inhibitor, camonsertib (RP-3500/RG6526)...
February 16, 2024: Research Square
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