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https://www.readbyqxmd.com/read/28723571/emi2-is-essential-for-mouse-spermatogenesis
#1
Lakshmi Gopinathan, Radoslaw Szmyd, Diana Low, M Kasim Diril, Heng-Yu Chang, Vincenzo Coppola, Kui Liu, Lino Tessarollo, Ernesto Guccione, Ans M M van Pelt, Philipp Kaldis
The meiotic functions of Emi2, an inhibitor of the APC/C complex, have been best characterized in oocytes where it mediates metaphase II arrest as a component of the cytostatic factor. We generated knockout mice to determine the in vivo functions of Emi2-in particular, its functions in the testis, where Emi2 is expressed at high levels. Male and female Emi2 knockout mice are viable but sterile, indicating that Emi2 is essential for meiosis but dispensable for embryonic development and mitotic cell divisions...
July 18, 2017: Cell Reports
https://www.readbyqxmd.com/read/28723232/mechanism-for-g2-phase-specific-nuclear-export-of-the-kinetochore-protein-cenp-f
#2
Kyle M Loftus, Heying Cui, Elias Coutavas, David S King, Amanda Ceravolo, Dylan Pereiras, Sozanne R Solmaz
Centromere protein F (CENP-F) is a component of the kinetochore and a regulator of cell cycle progression. CENP-F recruits the dynein transport machinery and orchestrates several cell cycle-specific transport events, including transport of the nucleus, mitochondria and chromosomes. A key regulatory step for several of these functions is likely the G2 phase-specific export of CENP-F from the nucleus to the cytosol, where the cytoplasmic dynein transport machinery resides; however, the molecular mechanism of this process is elusive...
July 19, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28722544/the-greatwall-kinase-is-dominant-over-pka-in-controlling-the-antagonistic-function-of-arpp19-in-xenopus-oocytes
#3
Aude-Isabelle Dupré, Olivier Haccard, Catherine Jessus
The small protein ARPP19 plays a dual role during oocyte meiosis resumption. In Xenopus, ARPP19 phosphorylation at S109 by PKA is necessary for maintaining oocytes arrested in prophase of the first meiotic division. Progesterone downregulates PKA, leading to the dephosphorylation of ARPP19 at S109. This initiates a transduction pathway ending with the activation of the universal inducer of M-phase, the kinase Cdk1. This last step depends on ARPP19 phosphorylation at S67 by the kinase Greatwall. Hence, phosphorylated by PKA at S109, ARPP19 restrains Cdk1 activation while when phosphorylated by Greatwall at S67, ARPP19 becomes an inducer of Cdk1 activation...
July 19, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28710122/correction-for-linares-et-al-phosphorylation-of-p62-by-cdk1-controls-the-timely-transit-of-cells-through-mitosis-and-tumor-cell-proliferation
#4
Juan F Linares, Ramars Amanchy, Kenneth Greis, Maria T Diaz-Meco, Jorge Moscat
No abstract text is available yet for this article.
August 1, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28708522/itraq-based-proteomic-profiling-of-granulosa-cells-from-lamb-and-ewe-after-superstimulation
#5
Jiapeng Lin, Yangsheng Wu, Bing Han, Ying Chen, Liqin Wang, Xiaolin Li, Mingjun Liu, Juncheng Huang
The number of oocytes obtained from lambs after FSH treatment is far greater than those acquired from adult ewes. However, these oocytes typically have reduced viability in comparison with adult ewe oocytes. However, the molecular mechanisms of differences in viability between lamb and ewe oocytes remain unknown. In the present research, we applied iTRAQ coupled with LC-MS/MS proteomic analysis in order to investigate the proteomic expression profile of granulosa cells from lambs and ewes following stimulation with FSH...
June 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/28706298/ckap2-phosphorylation-by-cdk1-cyclinb1-is-crucial-for-maintaining-centrosome-integrity
#6
Bum Ho Yoo, Du-Seock Kang, Chi-Hu Park, Kyeongjin Kang, Chang-Dae Bae
Previously, we have reported that CKAP2 is involved in the maintenance of centrosome integrity, thus allowing for proper mitosis in primary hepatocytes. To understand this biological process, we identified the mitosis-specific phosphorylation sites in mouse CKAP2 and investigated CKAP's possible role in cell cycle progression. Because we observed mouse CKAP2 depletion in amplified centrosomes and aberrant chromosomal segregation, which was rescued by ectopic expression of wild-type CKAP2, we focused on the centrosome duplication process among the various aspects of the cell cycle...
July 14, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28705199/the-m-phase-specific-hyperphosphorylation-of-staufen2-involved-the-cyclin-dependent-kinase-cdk1
#7
Rémy Beaujois, Elizabeth Ottoni, Xin Zhang, Christina Gagnon, Sami HSine, Stéphanie Mollet, Wildriss Viranaicken, Luc DesGroseillers
BACKGROUND: Staufen2 (STAU2) is an RNA-binding protein involved in the post-transcriptional regulation of gene expression. This protein was shown to be required for organ formation and cell differentiation. Although STAU2 functions have been reported in neuronal cells, its role in dividing cells remains deeply uncharacterized. Especially, its regulation during the cell cycle is completely unknown. RESULTS: In this study, we showed that STAU2 isoforms display a mitosis-specific slow migration pattern on SDS-gels in all tested transformed and untransformed cell lines...
July 14, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28700354/docking-of-cdk1-with-antibiotic-drugs-revealed-novel-therapeutic-value-in-breast-ductal-cancer-in-situ
#8
Zhong-Hai Ding, Jia Qi, An-Quan Shang, Yu-Jie Zhang, Jun Wei, Li-Qing Hu, Wei-Wei Wang, Man Yang
The aim of our research is to identify potential genes associated with Ductal carcinoma in situ (DCIS) through microarrays. The microarray dataset GS54665 were downloaded from the GEO(Gene Expression Omnibus) database. Dysregulated genes were screened and their associations with DCIS was analyzed by comprehensive bioinformatics tools. A total of 649 differential expression genes were identified between normal and DCIS samples, including 224 up-regulated genes and 425 down-regulated genes. Biological process annotation and pathway enrichment analysis identified several DCIS-related signaling pathways...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28694245/fundamental-cell-cycle-kinases-collaborate-to-ensure-timely-destruction-of-the-synaptonemal-complex-during-meiosis
#9
Bilge Argunhan, Wing-Kit Leung, Negar Afshar, Yaroslav Terentyev, Vijayalakshmi V Subramanian, Yasuto Murayama, Andreas Hochwagen, Hiroshi Iwasaki, Tomomi Tsubouchi, Hideo Tsubouchi
The synaptonemal complex (SC) is a proteinaceous macromolecular assembly that forms during meiotic prophase I and mediates adhesion of paired homologous chromosomes along their entire lengths. Although prompt disassembly of the SC during exit from prophase I is a landmark event of meiosis, the underlying mechanism regulating SC destruction has remained elusive. Here, we show that DDK (Dbf4-dependent Cdc7 kinase) is central to SC destruction. Upon exit from prophase I, Dbf4, the regulatory subunit of DDK, directly associates with and is phosphorylated by the Polo-like kinase Cdc5...
July 10, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28694172/macrophage-migration-inhibitory-factor-mediates-viability-and-apoptosis-of-pvm-ms-through-pi3k-akt-pathway
#10
Wenjing Zhang, Jian Zheng, Juan Meng, Lingling Neng, Xiaohua Chen, Zhaobing Qin
Macrophage migration inhibitory factor (MIF) plays an important role in hearing function; however, the underlying mechanism remains indistinct. PVM/Ms from the stria vascularis of lateral wall of cochlea in young and aged mice were isolated, and the mRNA and protein expression levels were detected. MIF was knocked down or overexpresssed in vitro, and transfection was performed in vivo. Cell viability and apoptosis were determined by MTT assay and flow cytometry analysis, respectively. The hearing ability was tested by the auditory brain stem response...
July 8, 2017: Neuroscience
https://www.readbyqxmd.com/read/28693630/developmental-competence-of-dromedary-camel-camelus-dromedarius-oocytes-selected-using-brilliant-cresyl-blue-staining
#11
Mohamed Fathi, Mohamed Ashry, Ali Salama, Magdy R Badr
The objectives of the present studies were to investigate the developmental capacity of dromedary camel oocytes selected by brilliant cresyl blue (BCB) staining and to investigate the expression of select transcripts in germinal vesicle (GV) stage oocytes. These transcripts included BMP15 and GDF9 as important transcripts for folliculogenesis and oocyte development, Zar1 and Mater as maternal transcripts required for embryonic development, Cyclin B1 and CDK1 as cell cycle regulators and Oct4 and STAT3 as transcription factors...
July 11, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28693383/saccharomyces-cerevisiae-msa1-mrna-has-a-sequence-for-localization-at-the-bud-tip
#12
Tomoko Takeuchi-Andoh, Yukiko Hayano-Oshiro, Emi Nishiyoshi, Masatoshi Mutazono, Sachiko Hayashi, Tokio Tani
MSA1 mRNA encodes Msa1p, a protein associated with the SCB-binding factor (SBF) and MCB-binding factor (MBF) complex. Msa1p promotes the transcription of G1 phase-specific genes, and is subjected to cell cycle-dependent regulation for its abundance and subcellular localization. MSA1 mRNA and Msa1p levels oscillate in the cell cycle with peaks at the late M/early G1 phase and early G1 phase, respectively. Phosphorylation by CDK1 negatively regulates the nuclear localization of Msa1p. In the present study, we identified MSA1 mRNA as a bud tip-localized mRNA in screening using a Tag-GFP system...
July 11, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28693202/ppeop-from-omphalia-lapidescens-schroet-induces-cell-cycle-arrest-and-inhibits-the-migration-of-mc-4-gastric-tumor-cells
#13
Yong-Le Yang, Wei-Yao Gong, Fei-Fei Chen, Lu-Chao Chen, Yi-Tao Chen
The aim of the present study was to investigate the effect of purified Omphalia lapidescens protein (pPeOp) extracted by polyvinylpyrrolidone from the fungus Omphalia lapidescens Schroet on the proliferation and cell cycle progression of MC-4 human gastric tumor cells. Using polyvinylpyrrolidone, pPeOp was extracted from O. lapidescens Schroet. MC-4 cells were cultured with 30, 60 or 90 µg/ml pPeOp, with 5-fluorouracil used as a positive control. Survival rates of treated cells were significantly decreased compared with those of the untreated control group in a dose-dependent manner...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28692435/prim-o-glucosylcimifugin-induces-cell-cycle-arrest-and-apoptosis-in-acute-lymphoblastic-leukemia-cells
#14
Lu Zhang, Xiaodi Liu, Xiongbin Lu, Cheng Huang
Prim-O-glucosylcimifugin is a major constituent in Radix Saposhnikovia that has been long used for the treatment of pyrexia, rheumatism, and cancer in traditional Chinese medicine. However, the molecular and cellular mechanisms remain unknown regarding the therapeutic effect of prim-O-lucosylcimifugin. Here, we investigated the effects of prim-O-glucosylcimifugin on cell cycle progression and apoptosis in human acute lymphoblastic leukemia cells. Prim-O-glucosylcimifugin treatment resulted in marked increases in cell apoptosis and cell cycle arrest at the G2/M phase...
July 7, 2017: Anti-cancer Drugs
https://www.readbyqxmd.com/read/28689916/molecular-mechanism-of-anti-cancerous-potential-of-morin-extracted-from-mulberry-in-hela-cells
#15
Qi Zhang, Fang Zhang, Kiran Thakur, Jun Wang, Hao Wang, Fei Hu, Jian-Guo Zhang, Zhao-Jun Wei
The flavonoids are of great interest due to their antioxidant and anti-cancerous potential. The present study investigated the Morin extracted from mulberry leaves and its anti-cancerous mechanism via cell inhibition, cell cycle analysis, apoptosis, mRNA expression and antioxidant mechanism through reactive oxygen species generation. Morin inhibited the proliferation of HeLa cells at IC50 of 214.28 μM and led to morphological changes, followed by induction of cell cycle arrest in G2/M-phase and ultimately resulted into apoptosis...
July 6, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28688277/synthesis-antitumor-activity-and-cdk1-inhibiton-of-new-thiazole-nortopsentin-analogues
#16
Barbara Parrino, Alessandro Attanzio, Virginia Spanò, Stella Cascioferro, Alessandra Montalbano, Paola Barraja, Luisa Tesoriere, Patrizia Diana, Girolamo Cirrincione, Anna Carbone
A new series of thiazole nortopsentin analogues was conveniently synthesized with fair overall yields. The antiproliferative activity of the new derivatives was tested against different human tumor cell lines of the NCI full panel. Four of them showed good antitumor activity with GI50 values from micro to nanomolar level. The mechanism of the antiproliferative effect of these derivatives, was pro-apoptotic, being associated with externalization of plasma membrane phosphatidylserine and DNA fragmentation. The most active and selective of the new thiazoles confined viable cells in G2/M phase and markedly inhibited in vitro CDK1 activity...
June 27, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28682437/over-expression-of-mir-1271-inhibits-endometrial-cancer-cells-proliferation-and-induces-cell-apoptosis-by-targeting-cdk1
#17
L Li, Y-W Qu, Y-P Li
OBJECTIVE: Endometrial carcinoma (EC) is one of the most common female malignancies worldwide. Growing evidence showed that microRNAs (miRNAs) are involved in the EC progression. The present study aimed to investigate the role of miR-1271 in the development and progression of EC. PATIENTS AND METHODS: The EC tissues and adjacent normal tissues were obtained from 42 EC patients. The expression of miR-1271 in EC tissues and cells was examined using Real-time RT-PCR...
June 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28682236/cell-cycle-entry-triggers-a-switch-between-two-modes-of-cdc42-activation-during-yeast-polarization
#18
Kristen L Witte, Devin Strickland, Michael Glotzer
Cell polarization underlies many cellular and organismal functions. The GTPase Cdc42 orchestrates polarization in many contexts. In budding yeast, polarization is associated with a focus of Cdc42•GTP which is thought to self sustain by recruiting a complex containing Cla4, a Cdc42-binding effector, Bem1, a scaffold, and Cdc24, a Cdc42 GEF. Using optogenetics, we probe yeast polarization and find that local recruitment of Cdc24 or Bem1 is sufficient to induce polarization by triggering self-sustaining Cdc42 activity...
July 6, 2017: ELife
https://www.readbyqxmd.com/read/28678347/trap1-controls-cell-cycle-g2-m-transition-through-the-regulation-of-cdk1-and-mad2-expression-ubiquitination
#19
Lorenza Sisinni, Francesca Maddalena, Valentina Condelli, Giuseppe Pannone, Vittorio Simeon, Valeria Li Bergolis, Elvira Lopes, Annamaria Piscazzi, Danilo Swann Matassa, Carmela Mazzoccoli, Filomena Nozza, Giacomo Lettini, Maria Rosaria Amoroso, Pantaleo Bufo, Franca Esposito, Matteo Landriscina
Regulation of tumor cell proliferation by molecular chaperones is still a complex issue. Here, the role of the HSP90 molecular chaperone TRAP1 in cell cycle regulation was investigated in a wide range of human breast, colorectal and lung carcinoma cell lines and tumor specimens. TRAP1 modulates the expression and/or the ubiquitination of key cell cycle regulators through a dual mechanism: i) a transcriptional regulation of CDK1, CYCLIN B1 and MAD2, as suggested by a gene expression profiling of TRAP1-silenced breast carcinoma cells, and ii) a post-transcriptional quality control of CDK1 and MAD2, being the ubiquitination of these two proteins enhanced upon TRAP1 downregulation...
July 5, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28676626/yeast-cip1-is-activated-by-environmental-stress-to-inhibit-cdk1-g1-cyclins-via-mcm1-and-msn2-4
#20
Ya-Lan Chang, Shun-Fu Tseng, Yu-Ching Huang, Zih-Jie Shen, Pang-Hung Hsu, Meng-Hsun Hsieh, Chia-Wei Yang, Silvia Tognetti, Berta Canal, Laia Subirana, Chien-Wei Wang, Hsiao-Tan Chen, Chi-Ying Lin, Francesc Posas, Shu-Chun Teng
Upon environmental changes, proliferating cells delay cell cycle to prevent further damage accumulation. Yeast Cip1 is a Cdk1 and Cln2-associated protein. However, the function and regulation of Cip1 are still poorly understood. Here we report that Cip1 expression is co-regulated by the cell-cycle-mediated factor Mcm1 and the stress-mediated factors Msn2/4. Overexpression of Cip1 arrests cell cycle through inhibition of Cdk1-G1 cyclin complexes at G1 stage and the stress-activated protein kinase-dependent Cip1 T65, T69, and T73 phosphorylation may strengthen the Cip1and Cdk1-G1 cyclin interaction...
July 4, 2017: Nature Communications
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