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https://www.readbyqxmd.com/read/28535485/quantification-of-residual-antibiotics-in-cow-manure-being-spread-over-agricultural-land-and-assessment-of-their-behavioral-effects-on-antibiotic-resistant-bacteria
#1
Ji-Ran Yeom, Soon-Uk Yoon, Chang-Gyun Kim
Antibiotic resistant bacteria (ARB) in livestock manure used as fertilizer and spread over agriculture land, may pose a threat to the health of humans. Considering this, the concentrations of tetracycline (TC), oxytetracycline (OTC), and sulfathiazole (STZ) in the surface soil were quantified using LC-MS. These antibiotics have been used in livestock and are found in fertilizer produced from livestock excretions. Species of ABR were identified using 16S rDNA. Soil samples were collected at depths of 0, 7, and 15 cm from farmland in Incheon (South Korea)...
May 16, 2017: Chemosphere
https://www.readbyqxmd.com/read/28535464/occurrence-of-marine-algal-toxins-in-oyster-and-phytoplankton-samples-in-daya-bay-south-china-sea
#2
Tao Jiang, Lei Liu, Yang Li, Jing Zhang, Zhijun Tan, Haiyan Wu, Tianjiu Jiang, Songhui Lu
The occurrence and seasonal variations of marine algal toxins in phytoplankton and oyster samples in Daya Bay (DYB), South China Sea were investigated. Two Dinophysis species, namely, D. caudata and D. acuminata complex, were identified as Okadaic acid (OA)/pectenotoxin (PTX) related species. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis demonstrated that 2.04-14.47 pg PTX2 per cell was the predominant toxin in single-cell isolates of D. caudata. D. acuminata was not subjected to toxin analysis...
May 12, 2017: Chemosphere
https://www.readbyqxmd.com/read/28535438/determination-of-edoxaban-equivalent-concentrations-in-human-plasma-by-an-automated-anti-factor-xa-chromogenic-assay
#3
Ling He, Jarema Kochan, Min Lin, Alexander Vandell, Karen Brown, Francois Depasse
INTRODUCTION: This phase I, open-label, multiple-dose, two-treatment study assessed the relationship between edoxaban equivalent concentration derived from an anti-FXa assay with the summed concentration of edoxaban and its active metabolite, M-4, as assessed by liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). This study also assessed the relationship between edoxaban plasma concentrations assessed by LC/MS/MS in sodium citrate and lithium heparin tubes. MATERIALS AND METHODS: Healthy volunteers were randomized to receive once-daily edoxaban 60mg or 90mg for 5days (15 participants per treatment group)...
May 7, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28534963/n-acetylglucosaminyltransferase-iva-promotes-invasion-of-choriocarcinoma
#4
Kimihiro Nishino, Eiko Yamamoto, Kaoru Niimi, Yoko Sekiya, Yoriko Yamashita, Fumitaka Kikkawa
Gestational trophoblastic neoplasia (GTN) results from the malignant transformation of placental trophoblasts which secrete human chorionic gonadotropin (hCG) as do normal placenta or hydatidiform mole. N-acetylglucosaminyltransferase IV (GnT-IV) is a glycosyltransferase which catalyses the formation of β1,4GlcNAc branches on the mannose core of N-glycans. Previous studies reported that β1,4GlcNAc branches on hCG were detected in GTN but not in normal pregnancy or hydatidiform mole. The aim of the present study was to understand the role of GnT-IVa in choriocarcinoma and find the target proteins for GnT-IVa glycosylation which contribute to the malignancy of choriocarcinoma...
May 23, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28534590/generation-of-wheat-gluten-hydrolysates-with-dipeptidyl-peptidase-iv-dpp-iv-inhibitory-properties
#5
A B Nongonierma, M Hennemann, S Paolella, R J FitzGerald
Wheat gluten, a Pro-rich dietary protein, was investigated for its potential to produce dipeptidyl peptidase IV (DPP-IV) inhibitory peptides during enzymatic hydrolysis with Debitrase HYW20. Nine gluten hydrolysates (H1-H9) were generated using a 2 factor × 3 level design of experiments (DOE) including the incubation temperature (40, 50 and 60 °C) and the enzyme: substrate ratio (E : S, 0.5, 1.0 and 1.5% (w/w)). Their DPP-IV half maximal inhibitory concentration (IC50) ranged from 0.24 ± 0.02 (H9) to 0...
May 23, 2017: Food & Function
https://www.readbyqxmd.com/read/28534470/development-and-validation-of-a-rapid-and-sensitive-lc-ms-ms-method-for-the-pharmacokinetic-study-of-osimertinib-in-rats
#6
Shan Xiong, Zhipeng Deng, Peilu Sun, Yanling Mu, Mingxing Xue
<p>Osimertinib is a new-generation epidermal growth factor inhibitor for the treatment of non-small cell lung cancer. In the present study, a rapid and sensitive LC with tandem MS method was developed and validated for the determination of osimertinib in rat plasma. Chromatographic separation was carried out on a C18 column using acetonitrile and water containing 0.1% formic acid. The assay was validated over a concentration range of 1.0–1000 ng/mL for osimertinib, with a lower LOQ of 1.0 ng/mL...
May 22, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28534130/tissue-distribution-and-dermal-drug-determination-of-indomethacin-transdermal-absorption-patches
#7
Jingjing Ma, Ying Gao, Yinghua Sun, Dawei Ding, Qi Zhang, Bingjun Sun, Menglin Wang, Jin Sun, Zhonggui He
The tissue distribution and percutaneous drug absorption of indomethacin (IND) patches were studied using commercial IND as a comparison. The concentration of IND in skin, plasma, and muscle in mice was measured by LC-MS/MS, and the IND concentration in the dermis of rats was also monitored by microdialysis. After percutaneous administration, the "double-peak" phenomenon occurred in different tissues, and the IND concentration was ranked as skin first, followed by plasma and then muscle. In particular, skin acted as a reservoir for drug release, and the "secondary hump" in tissue distribution was attributed to the subsequent release of lipophilic IND in skin...
May 22, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28533942/hla-a24-ligandome-analysis-of-colon-and-lung-cancer-cells-identifies-a-novel-cancer-testis-antigen-and-a-neoantigen-that-elicits-specific-and-strong-ctl-responses
#8
Vitaly Kochin, Takayuki Kanaseki, Serina Tokita, Sho Miyamoto, Yosuke Shionoya, Yasuhiro Kikuchi, Daichi Morooka, Yoshihiko Hirohashi, Tomohide Tsukahara, Kazue Watanabe, Shingo Toji, Yasuo Kokai, Noriyuki Sato, Toshihiko Torigoe
This study focused on HLA-A24 and comprehensively analyzed the ligandome of colon and lung cancer cells without the use of MHC-binding in silico prediction algorithms. Affinity purification using the antibody specific to HLA-A24 followed by LC-MS/MS sequencing was used to detect peptides, which harbored the known characteristics of HLA-A24 peptides in terms of length and anchor motifs. Ligandome analysis demonstrated the natural presentation of two different types of novel tumor-associated antigens. The ligandome contained a peptide derived from SUV39H2, a gene found to be expressed in a variety of cancers but not in normal tissues (except for the testis)...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28533511/cd47-surface-stability-is-sensitive-to-actin-disruption-prior-to-inclusion-within-the-band-3-macrocomplex
#9
Kathryn E Mordue, Bethan R Hawley, Timothy J Satchwell, Ashley M Toye
CD47 is an important 'marker of self' protein with multiple isoforms produced though alternative splicing that exhibit tissue-specific expression. Mature erythrocytes express CD47 isoform 2 only, with membrane stability of this version dependent on inclusion within the band 3 macrocomplex, via protein 4.2. At present a paucity of information exists regarding the associations and trafficking of the CD47 isoforms during erythropoiesis. We show that CD47 isoform 2 is the predominant version maintained at the surface of expanding and terminally differentiating erythroblasts...
May 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28533065/co-overexpression-of-response-regulator-genes-slr1037-and-sll0039-improves-tolerance-of-synechocystis-sp-pcc-6803-to-1-butanol
#10
Xinyan Gao, Tao Sun, Lina Wu, Lei Chen, Weiwen Zhang
In this study, two response regulator (RR) encoding genes slr1037 as well as sll0039 were co-overexpressed in Synechocystis sp. PCC 6803 by metabolic engineering and the 1-butanol tolerance was successfully improved by 133%. Aiming to explore the possible mechanisms for the enhancing 1-butanol tolerance, a quantitative iTRAQ-LC-MS/MS proteomics approach was then employed, identifying 216 up-regulated and 99 down-regulated proteins compared to wild type after 1-butanol treatment. This study mapped the potential target genes regulated by Slr1037 and Sll0039 and demonstrated the feasibility of engineering response regulators for modifying the biofuel tolerance in cyanobacteria...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28532150/synephrine-as-a-specific-marker-for-orange-consumption
#11
Matthias Bader, Tatjana Lang, Roman Lang, Thomas Hofmann
To validate the suitability of synephrine, known as a highly abundant alkaloid in oranges, as a dietary biomarker for orange consumption, a highly sensitive and robust stable isotope dilution analysis (SIDA) as well as an ECHO method, using the analyte itself as a pseudo-internal standard injected into the analysis run providing an "echo peak" of the analyte, was developed to quantitate synephrine by LC-MS/MS in citrus juices and human urine before and after ingestion of orange juice. A citrus juice screening revealed high synephrine concentrations of 150 to 420 nmol/mL in orange (n=11) and tangerine juices (n=2), while 20 to 100 times lower levels were found in juice from grapefruit (n=14), lemon (n=5) and lime (n=4), respectively...
May 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28532121/antimicrobial-activity-of-puffball-bovistella-radicata-and-separation-of-bioactive-compounds
#12
Yong Ye, Kun Liu, Qinghua Zeng, Qingmei Zeng
BACKGROUND: To test the antimicrobial activity of different extracts and fermentation broth from puffball(Bovistella radicata), the different extracts and fermentation broth of puffball were prepared, the active fraction was investigated by UPLC-UV-MS and semi-preparative chromatograph. RESULTS: Through zones of inhibition (ZOI) and minimum inhibitory concentrations (MIC) tests, the supernatant of fermentation possessed best antimicrobial activity in all extracts whose MIC value is 31...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28531831/workflow-methodology-for-rat-brain-metabolome-exploration-using-nmr-lc-ms-and-gc-ms-analytical-platforms
#13
Binta Diémé, Antoine Lefèvre, Lydie Nadal-Desbarats, Laurent Galineau, Blandine Madji Hounoum, Frédéric Montigny, Hélène Blasco, Christian R Andres, Patrick Emond, Sylvie Mavel
We developed a multi-platform approach for the metabolome exploration of rat brain tissue, using liquid chromatography coupled with mass spectrometry (LC-MS), nuclear magnetic resonance spectroscopy (NMR) and gas-chromatography coupled with mass spectrometry (GC-MS). The critical steps for metabolite exploration of cerebral tissues are tissue lysis and metabolites extraction. We first evaluated the impact of freeze-drying compared to wet tissue metabolites extraction using NMR and LC-MS with a reversed phase liquid chromatography...
May 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28531457/new-structural-insights-into-the-oligosaccharide-phosphate-fraction-of-pichia-hansenula-holstii-nrrl-y2448-phosphomannan
#14
Paul N Handley, Anthony Carroll, Vito Ferro
The oligosaccharide phosphate fraction (OPF) obtained from mild acid hydrolysis of P. holstii NRRL Y-2448 phosphomannan is the starting material for the preparation of the Phase III anticancer drug candidate PI-88. The OPF was for the first time successfully separated by preparative ion exchange chromatography and the major oligosaccharides isolated and characterized by NMR spectroscopy. The components were also acetylated and subjected to LC-MS analysis. These studies revealed that the OPF also contained all-α(1 → 3)-linked oligosaccharides in addition to the known α(1 → 3)/(1 → 2)-linked species, most likely formed by hydrolysis of the latter...
May 13, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28531327/interstrand-cross-links-arising-from-strand-breaks-at-true-abasic-sites-in-duplex-dna
#15
Zhiyu Yang, Nathan E Price, Kevin M Johnson, Yinsheng Wang, Kent S Gates
Interstrand cross-links are exceptionally bioactive DNA lesions. Endogenous generation of interstrand cross-links in genomic DNA may contribute to aging, neurodegeneration, and cancer. Abasic (Ap) sites are common lesions in genomic DNA that readily undergo spontaneous and amine-catalyzed strand cleavage reactions that generate a 2,3-didehydro-2,3-dideoxyribose sugar remnant (3'ddR5p) at the 3'-terminus of the strand break. Interestingly, this strand scission process leaves an electrophilic α,β-unsaturated aldehyde residue embedded within the resulting nicked duplex...
May 22, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28531177/geographical-trends-of-pfas-in-cod-livers-along-the-norwegian-coast
#16
Stig Valdersnes, Bente M Nilsen, Joar F Breivik, Asbjørn Borge, Amund Maage
The level of perfluorinated alkyl substances (PFAS) was determined in North East Arctic cod (Gadus morhua) liver samples from 15 Norwegian fjords and harbors. Five harbors in the eastern part of Norway, six harbors in the western part and four harbours in the northern part. A total of 200 samples were analyzed for 16 PFAS. Determination of PFAS were carried out by LC-MS/MS following sample clean up by solid phase extraction and ultracentrifugation. The predominating PFAS was PFOS, which was found to be higher than the level of quantification (1...
2017: PloS One
https://www.readbyqxmd.com/read/28530613/combination-of-solvent-extractants-for-dispersive-liquid-liquid-microextraction-of-fungicides-from-water-and-fruit-samples-by-liquid-chromatography-with-tandem-mass-spectrometry
#17
Marta Pastor-Belda, Isabel Garrido, Natalia Campillo, Pilar Viñas, Pilar Hellín, Pilar Flores, José Fenoll
A multiresidue method was developed to determine twenty-five fungicides belonging to three different chemical families, oxazoles, strobilurins and triazoles, in water and fruit samples, using dispersive liquid-liquid microextraction (DLLME) and liquid chromatography/tandem mass spectrometry (LC-MS(2)). Solid-liquid extraction with acetonitrile was used for the analysis in fruits, the extract being used as dispersant solvent in DLLME. Since some of the analytes showed high affinity for chloroform and the others were more efficiently extracted with undecanol, a mixture of both solvents was used as extractant in DLLME...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530609/phenolic-compounds-of-the-australian-native-herb-prostanthera-rotundifolia-and-their-biological-activities
#18
Kitty S C Tang, Izabela Konczak, Jian Zhao
The chemical identity and bioactivities of phenolic components of the Australian native herb Prostanthera rotundifolia were studied. Phenolic compounds were extracted with 80% (v/v) aqueous methanol and purified by liquid chromatography. The antioxidant capacity of the extract and its inhibiting activity against α-glucosidase, pancreatic lipase and hyaluronidase were determined. Phenolic compounds were identified by a combination of HPLC-PDA, LC-high resolution MS (LC-HRMS), LC-tandem MS (LC-MS/MS) and nuclear magnetic resonance (NMR) spectroscopy...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530589/comparison-of-hplc-ri-lc-ms-ms-and-enzymatic-assays-for-the-analysis-of-residual-lactose-in-lactose-free-milk
#19
A Trani, G Gambacorta, P Loizzo, A Cassone, C Fasciano, A V Zambrini, M Faccia
Lactose intolerance is the decreased ability to digest lactose, and the population involved is rapidly increasing all over the world. Different procedures have been reported in the literature to quantify lactose in dairy products, but the official method of analysis is based on enzymatic assay. In this paper, the effectiveness of two enzymatic kits in detecting residual lactose in lactose-free milk was investigated, and a comparison with two alternative chromatographic methods was done. The investigation used several samples of UHT milk containing different levels of lactose, and the results highlighted the inadequacy of the enzymatic assays and of the HPLC-RI method to analyse lactose-free milk...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530406/comparative-evaluation-of-small-molecular-additives-and-their-effects-on-peptide-protein-identification
#20
Jing Gao, Shaoyun Zhong, Yanting Zhou, Han He, Shuying Peng, Zhenyun Zhu, Xing Liu, Jing Zheng, Bin Xu, Hu Zhou
Detergents and salts are widely used in lysis buffers to enhance protein extraction from biological samples, facilitating in-depth proteomic analysis. However, these detergents and salt additives must be efficiently removed from the digested samples prior to LC-MS/MS analysis to obtain high-quality mass spectra. Although filter-aided sample preparation (FASP), acetone precipitation (AP), followed by in-solution digestion, and strong cation exchange-based centrifugal proteomic reactors (CPRs) are commonly used for proteomic sample processing, little is known about their efficiencies at removing detergents and salt additives...
May 22, 2017: Analytical Chemistry
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