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https://www.readbyqxmd.com/read/28094763/measuring-the-effect-of-femoral-malrotation-on-knee-joint-biomechanics-for-total-knee-arthroplasty-using-computational-simulation
#1
K-T Kang, Y-G Koh, J Son, O-R Kwon, C Baek, S H Jung, K K Park
OBJECTIVES: Malrotation of the femoral component can result in post-operative complications in total knee arthroplasty (TKA), including patellar maltracking. Therefore, we used computational simulation to investigate the influence of femoral malrotation on contact stresses on the polyethylene (PE) insert and on the patellar button as well as on the forces on the collateral ligaments. MATERIALS AND METHODS: Validated finite element (FE) models, for internal and external malrotations from 0° to 10° with regard to the neutral position, were developed to evaluate the effect of malrotation on the femoral component in TKA...
November 2016: Bone & Joint Research
https://www.readbyqxmd.com/read/28073404/isolation-and-characterization-of-hiv-1-envelope-glycoprotein-specific-b-cell-from-immortalized-human-na%C3%A3-ve-b-cell-library
#2
Zehua Sun, Shiqiang Lu, Zheng Yang, Jingjing Li, Meiyun Zhang
With the recent development of single B cell cloning techniques, an increasing number of HIV-1-specific broadly neutralizing antibodies (bNAbs) have been isolated since 2009. However, knowledge regarding HIV-1-specific B cells in vivo is limited. In this study, an HIV-1-specific B cell line has been established using healthy PBMC donors by the highly efficient EBV transformation method to generate immortalized human naïve B cell libraries. The enrichment of HIV-1 envelope-specific B cells was observed after four rounds of cell panning with the HIV-1 envelope glycoprotein...
January 10, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28056549/pegylated-long-circulating-liposomes-deliver-homoharringtonine-to-suppress-multiple-myeloma-cancer-stem-cells
#3
Miao Li, Fangfang Shi, Xiong Fei, Songyan Wu, Di Wu, Meng Pan, Shouhua Luo, Ning Gu, Jun Dou
The goal of this investigation was to evaluate the inhibiting effect of high proportion polyethyleneglycol of long-circulating homoharringtonine liposomes on RPMI8226 multiple myeloma cancer stem cells. The CD138(-)CD34(-) multiple myeloma cancer stem cells isolated from RPMI8226 cell line using magnetic activated cell sorting system were, respectively, incubated with the optimized formulation of polyethyleneglycol of long-circulating homoharringtonine liposomes and the homoharringtonine in vitro, and the multiple myeloma cancer stem cell proliferation, colony formation, and cell cycle were analyzed...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28048689/su-f-t-294-the-analysis-of-gamma-criteria-for-delta4-dosimetry-using-statistical-process-control
#4
S Cho, S Ahn, J Kim, K Park, W Choi, H Lee, E Lee, J Yoon, K Keum
PURPOSE: To evaluate the sensitivity of gamma criteria for patient-specific volumetric modulated arc therapy(VMAT) quality assurance of the Delta(4) dosimetry program using the statistical process control(SPC) methodology. METHODS: The authors selected 20 patient-specific VMAT QA cases which were undertaken MapCHECK and ArcCHECK with gamma pass rate better than 97%. The QAs data were collected Delta4 Phantom+ and Elekta Agility six megavolts without using an angle incrementer...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28044059/the-esc-e-z-complex-an-effector-of-response-to-ovarian-steroids-manifests-an-intrinsic-difference-in-cells-from-women-with-premenstrual-dysphoric-disorder
#5
N Dubey, J F Hoffman, K Schuebel, Q Yuan, P E Martinez, L K Nieman, D R Rubinow, P J Schmidt, D Goldman
Clinical evidence suggests that mood and behavioral symptoms in premenstrual dysphoric disorder (PMDD), a common, recently recognized, psychiatric condition among women, reflect abnormal responsivity to ovarian steroids. This differential sensitivity could be due to an unrecognized aspect of hormonal signaling or a difference in cellular response. In this study, lymphoblastoid cell line cultures (LCLs) from women with PMDD and asymptomatic controls were compared via whole-transcriptome sequencing (RNA-seq) during untreated (ovarian steroid-free) conditions and following hormone treatment...
January 3, 2017: Molecular Psychiatry
https://www.readbyqxmd.com/read/28027603/survival-implications-of-opioid-use-before-and-after-liver-transplantation
#6
Henry B Randall, Tarek Alhamad, Mark A Schnitzler, Zidong Zhang, Sophia Ford-Glanton, David A Axelrod, Dorry L Segev, Bertram L Kasiske, Gregory P Hess, Hui Yuan, Rosemary Ouseph, Krista L Lentine
BACKGROUND: Implications of prescription opioid use for outcomes after liver transplant have not been described. METHODS: We integrated national transplant registry data with records from a large pharmaceutical claims clearinghouse (2008-2014; n=26,925). Opioid fills on the waiting list were normalized to morphine equivalents (ME), and exposure was categorized as: >0 to 2 ME/day (level 1), >2 to 10 ME/day (level 2), >10 to 70 ME/day (level 3), and >70 ME/day (level 4)...
December 27, 2016: Liver Transplantation
https://www.readbyqxmd.com/read/28018364/nkg2a-expressing-natural-killer-cells-dominate-the-response-to-autologous-lymphoblastoid-cells-infected-with-epstein-barr-virus
#7
Olivia Hatton, Dara Marie Strauss-Albee, Nancy Q Zhao, Mikel D Haggadone, Judith Shanika Pelpola, Sheri M Krams, Olivia M Martinez, Catherine A Blish
Epstein-Barr virus (EBV) is a human γ-herpesvirus that establishes latency and lifelong infection in host B cells while achieving a balance with the host immune response. When the immune system is perturbed through immunosuppression or immunodeficiency, however, these latently infected B cells can give rise to aggressive B cell lymphomas. Natural killer (NK) cells are regarded as critical in the early immune response to viral infection, but their role in controlling expansion of infected B cells is not understood...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27987508/-analysis-of-anterolateral-approach-and-lateral-approach-for-the-treatment-of-coronal-shear-fracture-of-the-distal-humeral
#8
Y Li, Y J Cha, T Li, M Q Gong, X Y Jiang
OBJECTIVE: To treat the coronal shear fracture of the distal humeral during open reduction and internal fixation by anterolateral approach and lateral approach, and to analyze the advantage and disadvantage of each approach. METHODS: From September 2006 to July 2014, 10 patients with coronal fracture of the distal humeral were analyzed, who were all treated with Open Reduction and Internal Fixation (ORIF), 5 with anterolateral approach (group A) and 5 with lateral approach (group B)...
December 18, 2016: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/27956246/global-profiling-of-viral-and-cellular-non-coding-rnas-in-epstein-barr-virus-induced-lymphoblastoid-cell-lines-and-released-exosome-cargos
#9
Alessia Gallo, Serena Vella, Monica Miele, Francesca Timoneri, Mariangela Di Bella, Silvia Bosi, Marco Sciveres, Pier Giulio Conaldi
The human EBV-transformed lymphoblastoid cell line (LCL), obtained by infecting peripheral blood monocular cells with Epstein-Barr Virus, has been extensively used for human genetic, pharmacogenomic, and immunologic studies. Recently, the role of exosomes has also been indicated as crucial in the crosstalk between EBV and the host microenvironment. Because the role that the LCL and LCL exosomal cargo might play in maintaining persistent infection, and since little is known regarding the non-coding RNAs of LCL, the aim of our work was the comprehensive characterization of this class of RNA, cellular and viral miRNAs, and cellular lncRNAs, in LCL compared with PBMC derived from the same donors...
December 10, 2016: Cancer Letters
https://www.readbyqxmd.com/read/27932332/the-anterolateral-capsule-of-the-knee-behaves-like-a-sheet-of-fibrous-tissue
#10
Daniel Guenther, Amir A Rahnemai-Azar, Kevin M Bell, Sebastián Irarrázaval, Freddie H Fu, Volker Musahl, Richard E Debski
BACKGROUND: The function of the anterolateral capsule of the knee has not been clearly defined. However, the contribution of this region of the capsule to knee stability in comparison with other anterolateral structures can be determined by the relative force that each structure carries during loading of the knee. PURPOSE/HYPOTHESIS: The purpose of this study was to determine the forces in the anterolateral structures of the intact and anterior cruciate ligament (ACL)-deficient knee in response to an anterior tibial load and internal tibial torque...
December 8, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27903901/core-binding-factor-cbf-is-required-for-epstein-barr-virus-ebna3-proteins-to-regulate-target-gene-expression
#11
Kostas Paschos, Quentin Bazot, Guiyi Ho, Gillian A Parker, Jonathan Lees, Geraint Barton, Martin J Allday
ChIP-seq performed on lymphoblastoid cell lines (LCLs), expressing epitope-tagged EBNA3A, EBNA3B or EBNA3C from EBV-recombinants, revealed important principles of EBNA3 binding to chromatin. When combined with global chromatin looping data, EBNA3-bound loci were found to have a singular character, each directly associating with either EBNA3-repressed or EBNA3-activated genes, but not with both. EBNA3A and EBNA3C showed significant association with repressed and activated genes. Significant direct association for EBNA3B loci could only be shown with EBNA3B-repressed genes...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27887872/optimizing-the-rehabilitation-of-elbow-lateral-collateral-ligament-injuries-a-biomechanical-study
#12
Ranita H K Manocha, Jonathan R Kusins, James A Johnson, Graham J W King
BACKGROUND: Elbow lateral collateral ligament (LCL) injury may arise after trauma or lateral surgical approaches. The optimal method of rehabilitating the LCL-insufficient elbow is unclear. Therapists often prescribe active motion exercises with the forearm pronated. Recently, overhead exercises have become popular as they may enable gravity to compress the elbow joint, improving stability, although this has not been proved biomechanically. This investigation aimed to quantify the effects of several variables used in LCL injury rehabilitation on elbow stability...
November 22, 2016: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/27876849/characterization-of-two-distinct-early-post-entry-blocks-to-hiv-1-in-common-marmoset-lymphocytes
#13
Beatriz Pacheco, Luis Menéndez-Arias, Joseph Sodroski
In nature, primate lentiviruses infect humans and several Old World monkeys and apes. However, to date, lentiviruses infecting New World monkeys have not been described. We studied the susceptibility of common marmoset cells to HIV-1 infection and observed the presence of post-entry blocks to the early phase of HIV-1 infection in peripheral blood lymphocytes (PBLs) and a B lymphocytic cell line (B-LCL). The blocks present in these cells are dominant and phenotypically different from each other. In PBLs, the block occurs at the level of reverse transcription, reducing the accumulation of early and late transcripts, similar to the block imposed by TRIM5α...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27876498/associated-lateral-medial-knee-instability-and-its-relevant-factors-in-anterior-cruciate-ligament-injured-knees
#14
Arata Yuuki, Takeshi Muneta, Toshiyuki Ohara, Ichiro Sekiya, Hideyuki Koga
BACKGROUND: Associations of lateral/medial knee instability with anterior cruciate ligament (ACL) injury have not been thoroughly investigated. The purposes of this study were to investigate whether lateral/medial knee instability is associated with ACL injury, and to clarify relevant factors for lateral/medial knee instability in ACL-injured knees. METHODS: One hundred and nineteen patients with unilateral ACL-injured knees were included. Lateral/medial knee instability was assessed with varus/valgus stress X-ray examination for both injured and uninjured knees by measuring varus/valgus angle, lateral/medial joint opening, and lateral/medial joint opening index...
November 19, 2016: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/27864512/epstein-barr-virus-super-enhancer-ernas-are-essential-for-myc-oncogene-expression-and-lymphoblast-proliferation
#15
Jun Liang, Hufeng Zhou, Catherine Gerdt, Min Tan, Tyler Colson, Kenneth M Kaye, Elliott Kieff, Bo Zhao
Epstein-Barr virus (EBV) super-enhancers (ESEs) are essential for lymphoblastoid cell (LCL) growth and survival. Reanalyses of LCL global run-on sequencing (Gro-seq) data found abundant enhancer RNAs (eRNAs) being transcribed at ESEs. Inactivation of ESE components, EBV nuclear antigen 2 (EBNA2) and bromodomain-containing protein 4 (BRD4), significantly decreased eRNAs at ESEs -428 and -525 kb upstream of the MYC oncogene transcription start site (TSS). shRNA knockdown of the MYC -428 and -525 ESE eRNA caused LCL growth arrest and reduced cell growth...
December 6, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27859544/lateral-soft-tissue-structures-contribute-to-cruciate-retaining-total-knee-arthroplasty-stability
#16
Kiron K Athwal, Hadi El Daou, Breck Lord, Andrew J Davies, William Manning, Ferdinando Rodriguez Y Baena, David J Deehan, Andrew A Amis
Little information is available to surgeons regarding how the lateral structures prevent instability in the replaced knee. The aim of this study was to quantify the lateral soft-tissue contributions to stability following cruciate-retaining total knee arthroplasty (CR TKA). Nine cadaveric knees were tested in a robotic system at full extension, 30°, 60°, and 90° flexion angles. In both native and CR implanted states, ±90 N anterior-posterior force, ±8 Nm varus-valgus, and ±5 Nm internal-external torque were applied...
November 9, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27824873/evaluating-varied-label-designs-for-use-with-medical-devices-optimized-labels-outperform-existing-labels-in-the-correct-selection-of-devices-and-time-to-select
#17
Laura Bix, Do Chan Seo, Moslem Ladoni, Eric Brunk, Mark W Becker
PURPOSE: Effective standardization of medical device labels requires objective study of varied designs. Insufficient empirical evidence exists regarding how practitioners utilize and view labeling. OBJECTIVE: Measure the effect of graphic elements (boxing information, grouping information, symbol use and color-coding) to optimize a label for comparison with those typical of commercial medical devices. DESIGN: Participants viewed 54 trials on a computer screen...
2016: PloS One
https://www.readbyqxmd.com/read/27813226/activation-of-an-au-cl-bond-by-a-pendent-sb-iii-lewis-acid-impact-on-structure-and-catalytic-activity
#18
J Stuart Jones, François P Gabbai
With the objective of identifying new coordination modes of ambiphilic ligands, we have investigated the bidentate Sb/P ligands (o-(Ph2 P)C6 H4 )SbCl2 (L(Cl) ) and (o-(Ph2 P)C6 H4 )SbPh2 (L(Ph) ). Reaction of these ligands with (tht)AuCl affords the monoligated species L(Cl) AuCl (1) and L(Ph) AuCl (2), respectively, in which the antimony centers are only weakly engaged with the coordinated gold atom. Treatment of 1 with PPh3 induces an intramolecular transfer of a chloride ligand from gold to antimony to form the zwitterionic species o-(Cl3 Sb)C6 H4 (Ph2 P)Au(PPh3 ) (3)...
November 4, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27802925/effects-of-suture-site-or-penetration-depth-on-anchor-location-in-all-inside-meniscal-repair
#19
Ryohei Uchida, Tatsuo Mae, Kunihiko Hiramatsu, Ryo Iuchi, Kazutaka Kinugasa, Konsei Shino, Hideki Yoshikawa, Ken Nakata
BACKGROUND: To evaluate the effects of suture site or penetration depth on anchor location in all-inside meniscal repair. METHODS: Eight fresh-frozen cadaveric knees were evaluated after meniscal repair using eight FasT-Fix360 (FF360) devices (Smith & Nephew Endoscopy, Andover, MA) (16 anchors) for each knee. The penetration depth was 14mm, the distance same from the periphery to insertion point, in four knees (Group A) and that in the remaining four knees (Group B) was 18mm...
December 2016: Knee
https://www.readbyqxmd.com/read/27802222/metabolic-and-growth-rate-alterations-in-lymphoblastic-cell-lines-discriminate-between-down-syndrome-and-alzheimer-s-disease
#20
Pinar Coskun, Pablo Helguera, Zahra Nemati, Ryan C Bohannan, Jean Thomas, Schriner E Samuel, Jocelyn Argueta, Eric Doran, Douglas C Wallace, Ira T Lott, Jorge Busciglio
BACKGROUND: Deficits in mitochondrial function and oxidative stress play pivotal roles in Down syndrome (DS) and Alzheimer's disease (AD) and these alterations in mitochondria occur systemically in both conditions. OBJECTIVE: We hypothesized that peripheral cells of elder subjects with DS exhibit disease-specific and dementia-specific metabolic features. To test this, we performed a comprehensive analysis of energy metabolism in lymphoblastic-cell-lines (LCLs) derived from subjects belonging to four groups: DS-with-dementia (DSAD), DS-without-dementia (DS), sporadic AD, and age-matched controls...
2017: Journal of Alzheimer's Disease: JAD
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