keyword
MENU ▼
Read by QxMD icon Read
search

LCL

keyword
https://www.readbyqxmd.com/read/27903901/core-binding-factor-cbf-is-required-for-epstein-barr-virus-ebna3-proteins-to-regulate-target-gene-expression
#1
Kostas Paschos, Quentin Bazot, Guiyi Ho, Gillian A Parker, Jonathan Lees, Geraint Barton, Martin J Allday
ChIP-seq performed on lymphoblastoid cell lines (LCLs), expressing epitope-tagged EBNA3A, EBNA3B or EBNA3C from EBV-recombinants, revealed important principles of EBNA3 binding to chromatin. When combined with global chromatin looping data, EBNA3-bound loci were found to have a singular character, each directly associating with either EBNA3-repressed or EBNA3-activated genes, but not with both. EBNA3A and EBNA3C showed significant association with repressed and activated genes. Significant direct association for EBNA3B loci could only be shown with EBNA3B-repressed genes...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27887872/optimizing-the-rehabilitation-of-elbow-lateral-collateral-ligament-injuries-a-biomechanical-study
#2
Ranita H K Manocha, Jonathan R Kusins, James A Johnson, Graham J W King
BACKGROUND: Elbow lateral collateral ligament (LCL) injury may arise after trauma or lateral surgical approaches. The optimal method of rehabilitating the LCL-insufficient elbow is unclear. Therapists often prescribe active motion exercises with the forearm pronated. Recently, overhead exercises have become popular as they may enable gravity to compress the elbow joint, improving stability, although this has not been proved biomechanically. This investigation aimed to quantify the effects of several variables used in LCL injury rehabilitation on elbow stability...
November 22, 2016: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/27876849/characterization-of-two-distinct-early-post-entry-blocks-to-hiv-1-in-common-marmoset-lymphocytes
#3
Beatriz Pacheco, Luis Menéndez-Arias, Joseph Sodroski
In nature, primate lentiviruses infect humans and several Old World monkeys and apes. However, to date, lentiviruses infecting New World monkeys have not been described. We studied the susceptibility of common marmoset cells to HIV-1 infection and observed the presence of post-entry blocks to the early phase of HIV-1 infection in peripheral blood lymphocytes (PBLs) and a B lymphocytic cell line (B-LCL). The blocks present in these cells are dominant and phenotypically different from each other. In PBLs, the block occurs at the level of reverse transcription, reducing the accumulation of early and late transcripts, similar to the block imposed by TRIM5α...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27876498/associated-lateral-medial-knee-instability-and-its-relevant-factors-in-anterior-cruciate-ligament-injured-knees
#4
Arata Yuuki, Takeshi Muneta, Toshiyuki Ohara, Ichiro Sekiya, Hideyuki Koga
BACKGROUND: Associations of lateral/medial knee instability with anterior cruciate ligament (ACL) injury have not been thoroughly investigated. The purposes of this study were to investigate whether lateral/medial knee instability is associated with ACL injury, and to clarify relevant factors for lateral/medial knee instability in ACL-injured knees. METHODS: One hundred and nineteen patients with unilateral ACL-injured knees were included. Lateral/medial knee instability was assessed with varus/valgus stress X-ray examination for both injured and uninjured knees by measuring varus/valgus angle, lateral/medial joint opening, and lateral/medial joint opening index...
November 19, 2016: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/27864512/epstein-barr-virus-super-enhancer-ernas-are-essential-for-myc-oncogene-expression-and-lymphoblast-proliferation
#5
Jun Liang, Hufeng Zhou, Catherine Gerdt, Min Tan, Tyler Colson, Kenneth M Kaye, Elliott Kieff, Bo Zhao
Epstein-Barr virus (EBV) super-enhancers (ESEs) are essential for lymphoblastoid cell (LCL) growth and survival. Reanalyses of LCL global run-on sequencing (Gro-seq) data found abundant enhancer RNAs (eRNAs) being transcribed at ESEs. Inactivation of ESE components, EBV nuclear antigen 2 (EBNA2) and bromodomain-containing protein 4 (BRD4), significantly decreased eRNAs at ESEs -428 and -525 kb upstream of the MYC oncogene transcription start site (TSS). shRNA knockdown of the MYC -428 and -525 ESE eRNA caused LCL growth arrest and reduced cell growth...
November 18, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27859544/lateral-soft-tissue-structures-contribute-to-cruciate-retaining-total-knee-arthroplasty-stability
#6
Kiron K Athwal, Hadi El Daou, Breck Lord, Andrew J Davies, William Manning, Ferdinando Rodriguez Y Baena, David J Deehan, Andrew A Amis
Little information is available to surgeons regarding how the lateral structures prevent instability in the replaced knee. The aim of this study was to quantify the lateral soft-tissue contributions to stability following cruciate-retaining total knee arthroplasty (CR TKA). Nine cadaveric knees were tested in a robotic system at full extension, 30°, 60°, and 90° flexion angles. In both native and CR implanted states, ±90 N anterior-posterior force, ±8 Nm varus-valgus, and ±5 Nm internal-external torque were applied...
November 9, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27824873/evaluating-varied-label-designs-for-use-with-medical-devices-optimized-labels-outperform-existing-labels-in-the-correct-selection-of-devices-and-time-to-select
#7
Laura Bix, Do Chan Seo, Moslem Ladoni, Eric Brunk, Mark W Becker
PURPOSE: Effective standardization of medical device labels requires objective study of varied designs. Insufficient empirical evidence exists regarding how practitioners utilize and view labeling. OBJECTIVE: Measure the effect of graphic elements (boxing information, grouping information, symbol use and color-coding) to optimize a label for comparison with those typical of commercial medical devices. DESIGN: Participants viewed 54 trials on a computer screen...
2016: PloS One
https://www.readbyqxmd.com/read/27813226/activation-of-an-au-cl-bond-by-a-pendent-sb-iii-lewis-acid-impact-on-structure-and-catalytic-activity
#8
James Stuart Jones, Francois Pierre Gabbai
With the objective of identifying new coordination modes of ambiphilic ligands, we have investigated the bidentate Sb/P ligands (o-(Ph2P)C6H4)SbCl2 (LCl) and (o-(Ph2P)C6H4)SbPh2 (LPh). Reaction of these ligands with (tht)AuCl affords the monoligated species LClAuCl (1) and LPhAuCl (2), respectively, in which the antimony centers are only weakly engaged with the coordinated gold atom. Treatment of 1 with PPh3 induces an intramolecular transfer of a chloride ligand from gold to antimony to form the zwitterionic species o-(Cl3Sb)C6H4(Ph2P)Au(PPh3) (3)...
November 4, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27802925/effects-of-suture-site-or-penetration-depth-on-anchor-location-in-all-inside-meniscal-repair
#9
Ryohei Uchida, Tatsuo Mae, Kunihiko Hiramatsu, Ryo Iuchi, Kazutaka Kinugasa, Konsei Shino, Hideki Yoshikawa, Ken Nakata
BACKGROUND: To evaluate the effects of suture site or penetration depth on anchor location in all-inside meniscal repair. METHODS: Eight fresh-frozen cadaveric knees were evaluated after meniscal repair using eight FasT-Fix360 (FF360) devices (Smith & Nephew Endoscopy, Andover, MA) (16 anchors) for each knee. The penetration depth was 14mm, the distance same from the periphery to insertion point, in four knees (Group A) and that in the remaining four knees (Group B) was 18mm...
October 29, 2016: Knee
https://www.readbyqxmd.com/read/27802222/metabolic-and-growth-rate-alterations-in-lymphoblastic-cell-lines-discriminate-between-down-syndrome-and-alzheimer-s-disease
#10
Pinar Coskun, Pablo Helguera, Zahra Nemati, Ryan C Bohannan, Jean Thomas, Schriner E Samuel, Jocelyn Argueta, Eric Doran, Douglas C Wallace, Ira T Lott, Jorge Busciglio
BACKGROUND: Deficits in mitochondrial function and oxidative stress play pivotal roles in Down syndrome (DS) and Alzheimer's disease (AD) and these alterations in mitochondria occur systemically in both conditions. OBJECTIVE: We hypothesized that peripheral cells of elder subjects with DS exhibit disease-specific and dementia-specific metabolic features. To test this, we performed a comprehensive analysis of energy metabolism in lymphoblastic-cell-lines (LCLs) derived from subjects belonging to four groups: DS-with-dementia (DSAD), DS-without-dementia (DS), sporadic AD, and age-matched controls...
October 11, 2016: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/27788618/optimization-of-prednisolone-loaded-long-circulating-liposomes-via-application-of-quality-by-design-qbd-approach
#11
Bianca Sylvester, Alina Porfire, Dana-Maria Muntean, Laurian Vlase, Lavinia Lupuţ, Emilia Licarete, Alina Sesarman, Marius Costel Alupei, Manuela Banciu, Marcela Achim, Ioan Tomuţă
Quality by design principles (QbD) were used to assist the formulation of prednisolone-loaded long-circulating liposomes (LCL-PLP) in order to gain a more comprehensive understanding of the preparation process. This approach enables us to improve the final product quality in terms of liposomal drug concentration, encapsulation efficiency and size, and to minimize preparation variability. A 19-run D-optimal experimental design was used to study the impact of the highest risk factors on PLP liposomal concentration (Y1- μg/ml), encapsulation efficiency (Y2-%) and size (Y3-nm)...
November 16, 2016: Journal of Liposome Research
https://www.readbyqxmd.com/read/27760751/phospholipid-profile-and-distribution-in-the-receptive-oviduct-and-uterus-during-early-diestrus-in-cattle
#12
Katia Roberta A Belaz, Alessandra Tata, Moana R França, Maressa I Santos da Silva, Pedro Henrique Vendramini, Anna Maria A P Fernandes, Fábio L D'Alexandri, Marcos N Eberlin, Mario Binelli
Phospholipid metabolism and signaling influences on early pregnancy events in cattle are unknown. This study aimed to characterize global phospholipid composition of oviduct and uterus during early diestrus in a model of contrasting embryo receptivity. Beef cows were treated to ovulate a larger (LF-LCL group, associated with greater receptivity) or smaller follicle (SF-SCL group) and, consequently, to present greater or smaller plasma concentrations of estradiol during proestrus-estrus as well as progesterone during early diestrus...
October 19, 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/27757317/highly-efficient-il-21-and-feeder-cell-driven-ex-vivo-expansion-of-human-nk-cells-with-therapeutic-activity-in-a-xenograft-mouse-model-of-melanoma
#13
Markus Granzin, Ana Stojanovic, Matthias Miller, Richard Childs, Volker Huppert, Adelheid Cerwenka
Natural killer (NK) cells are promising antitumor effector cells, but the generation of sufficient NK cell numbers for adoptive immunotherapy remains challenging. Therefore, we developed a method for highly efficient ex vivo expansion of human NK cells. Ex vivo expansion of NK cells in medium containing IL-2 and irradiated clinical-grade feeder cells (EBV-LCL) induced a 22-fold NK cell expansion after one week that was significantly increased to 53-fold by IL-21. Repeated stimulation with irradiated EBV-LCL and IL-2 and addition of IL-21 at the initiation of the culture allowed sustained NK cell proliferation with 10(11)-fold NK cell expansion after 6 weeks...
2016: Oncoimmunology
https://www.readbyqxmd.com/read/27754873/dynamic-remodeling-of-endometrial-extracellular-matrix-regulates-embryo-receptivity-in-cattle
#14
Saara Carollina Scolari, Guilherme Pugliesi, Ricardo de Francisco Strefezzi, Sónia Cristina Andrade, Luiz Lehmann Coutinho, Mario Binelli
We aimed to evaluate in the bovine endometrium whether (1) key genes involved in endometrial extracellular matrix (ECM) remodeling are regulated by the endocrine peri-ovulatory milieu; and (2) specific endometrial ECM-related transcriptome can be linked to pregnancy outcome. In Experiment 1, pre-ovulatory follicle growth of cows was manipulated to obtain two groups with specific endocrine peri-ovulatory profiles: the Large Follicle-Large CL group (LF-LCL) served as a paradigm for greater receptivity and fertility and showed greater plasma pre-ovulatory estradiol and post-ovulatory progesterone concentrations when compared to the Small Follicle-Small CL group (SF-SCL)...
October 17, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27716287/modeling-neurological-diseases-with-induced-pluripotent-cells-reprogrammed-from-immortalized-lymphoblastoid-cell-lines
#15
Koki Fujimori, Toshiki Tezuka, Hiroyuki Ishiura, Jun Mitsui, Koichiro Doi, Jun Yoshimura, Hirobumi Tada, Takuya Matsumoto, Miho Isoda, Ryota Hashimoto, Nubutaka Hattori, Takuya Takahashi, Shinichi Morishita, Shoji Tsuji, Wado Akamatsu, Hideyuki Okano
Patient-specific induced pluripotent stem cells (iPSCs) facilitate understanding of the etiology of diseases, discovery of new drugs and development of novel therapeutic interventions. A frequently used starting source of cells for generating iPSCs has been dermal fibroblasts (DFs) isolated from skin biopsies. However, there are also numerous repositories containing lymphoblastoid B-cell lines (LCLs) generated from a variety of patients. To date, this rich bioresource of LCLs has been underused for generating iPSCs, and its use would greatly expand the range of targeted diseases that could be studied by using patient-specific iPSCs...
October 3, 2016: Molecular Brain
https://www.readbyqxmd.com/read/27650967/novel-compound-heterozygous-variants-in-plk4-identified-in-a-patient-with-autosomal-recessive-microcephaly-and-chorioretinopathy
#16
Makiko Tsutsumi, Setsuri Yokoi, Fuyuki Miya, Masafumi Miyata, Mitsuhiro Kato, Nobuhiko Okamoto, Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Shinji Saitoh, Hiroki Kurahashi
It has been well documented that variants in genes encoding centrosomal proteins cause primary autosomal recessive microcephaly, although the association between centrosomal defects and the etiology of microcephaly syndromes is not fully understood. Polo-like kinase 4 (PLK4) is one of the centrosomal proteins required for centriole duplication. We here describe a patient with microcephaly and chorioretinopathy that harbors compound heterozygous missense variants, c.[442A>G]; [2336G>A], in the PLK4 gene...
September 21, 2016: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/27629864/decreased-photosynthesis-and-growth-with-reduced-respiration-in-the-model-diatom-phaeodactylum-tricornutum-grown-under-elevated-co2-over-1800-generations
#17
Futian Li, John Beardall, Sinéad Collins, Kunshan Gao
Studies on the long-term responses of marine phytoplankton to ongoing ocean acidification (OA) are appearing rapidly in the literature. However, only a few of these have investigated diatoms, which is disproportionate to their contribution to global primary production. Here we show that a population of the model diatom Phaeodactylum tricornutum, after growing under elevated CO2 (1000 μatm, HCL, pHT : 7.70) for 1860 generations, showed significant differences in photosynthesis and growth from a population maintained in ambient CO2 and then transferred to elevated CO2 for 20 generations (HC)...
September 15, 2016: Global Change Biology
https://www.readbyqxmd.com/read/27628546/-cancer-immunotherapy-using-human-induced-pluripotent-stem-cell-derived-dendritic-cells-ipsdcs-expressing-carcinoembryonic-antigen
#18
Junya Kitadani, Toshiyasu Ojima, Hiromitsu Iwamoto, Hiroaki Tabata, Mikihito Nakamori, Masaki Nakamura, Masahiro Katsuda, Motoki Miyazawa, Keiji Hayata, Hiroki Yamaue
The difficulty in obtaining a sufficient number of functional dendritic cells(DCs)is a well-known serious problem in DCbased immunotherapy. Therefore, we used induced pluripotent stem cell-derived DCs(iPSDCs). We have reported that mouse iPSDCs are equivalent to BMDCs, in terms of maturation and antigen presentation. In this study, the antitumor immune response of human iPSDCs expressing the carcinoembryonic antigen was examined, to determine its clinical application in gastrointestinal cancer. Human iPS cells were established from healthy human fibroblasts using a Sendai virus vector, and human iPSDCs were differentiated under a feeder-free culture...
September 2016: Gan to Kagaku Ryoho. Cancer & Chemotherapy
https://www.readbyqxmd.com/read/27616566/metformin-pharmacogenomics-a-genome-wide-association-study-to-identify-genetic-and-epigenetic-biomarkers-involved-in-metformin-anticancer-response-using-human-lymphoblastoid-cell-lines
#19
Nifang Niu, Tongzheng Liu, Junmei Cairns, Reynold C Ly, Xianglin Tan, Min Deng, Brooke L Fridley, Krishna R Kalari, Ryan P Abo, Gregory Jenkins, Anthony Batzler, Erin E Carlson, Poulami Barman, Sebastian Moran, Holger Heyn, Manel Esteller, Liewei Wang
Metformin is currently considered as a promising anticancer agent in addition to its anti-diabetic effect. To better individualize metformin therapy and explore novel molecular mechanisms in cancer treatment, we conducted a pharmacogenomic study using 266 lymphoblastoid cell lines (LCLs). Metformin cytotoxicity assay was performed using the MTS assay. Genome-wide association (GWA) analyses were performed in LCLs using 1.3 million SNPs, 485k DNA methylation probes, 54k mRNA expression probe sets, and metformin cytotoxicity (IC50s)...
September 11, 2016: Human Molecular Genetics
https://www.readbyqxmd.com/read/27600570/-chronic-ligamentous-instability-of-the-elbow
#20
M Hackl, T Leschinger, L P Müller, K Wegmann
BACKGROUND: Chronic ligamentous instability of the elbow is an important pathology as it is accompanied with pronounced dysfunction. Moreover, it represents an established risk factor for the development of osteoarthritis. Posterolateral rotatory instability (PLRI) caused by insufficiency of the lateral collateral ligament (LCL) is the most common type of chronic elbow instability and is usually a sequel of traumatic elbow dislocation. Chronic overload can lead to insufficiency of the ulnar collateral ligament (UCL) with subsequent valgus instability, especially in overhead athletes...
October 2016: Der Orthopäde
keyword
keyword
33962
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"