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Katia Roberta A Belaz, Alessandra Tata, Moana R França, Maressa I Santos da Silva, Pedro Henrique Vendramini, Anna Maria A P Fernandes, Fábio L D'Alexandri, Marcos N Eberlin, Mario Binelli
Phospholipid metabolism and signaling influences on early pregnancy events in cattle are unknown. This study aimed to characterize global phospholipid composition of oviduct and uterus during early diestrus in a model of contrasting embryo receptivity. Beef cows were treated to ovulate a larger (LF-LCL group, associated with greater receptivity) or smaller follicle (SF-SCL group) and, consequently, to present greater or smaller plasma concentrations of estradiol during proestrus-estrus as well as progesterone during early diestrus...
October 19, 2016: Biology of Reproduction
Markus Granzin, Ana Stojanovic, Matthias Miller, Richard Childs, Volker Huppert, Adelheid Cerwenka
Natural killer (NK) cells are promising antitumor effector cells, but the generation of sufficient NK cell numbers for adoptive immunotherapy remains challenging. Therefore, we developed a method for highly efficient ex vivo expansion of human NK cells. Ex vivo expansion of NK cells in medium containing IL-2 and irradiated clinical-grade feeder cells (EBV-LCL) induced a 22-fold NK cell expansion after one week that was significantly increased to 53-fold by IL-21. Repeated stimulation with irradiated EBV-LCL and IL-2 and addition of IL-21 at the initiation of the culture allowed sustained NK cell proliferation with 10(11)-fold NK cell expansion after 6 weeks...
2016: Oncoimmunology
Saara Carollina Scolari, Guilherme Pugliesi, Ricardo de Francisco Strefezzi, Sónia Cristina Andrade, Luiz Lehmann Coutinho, Mario Binelli
We aimed to evaluate in the bovine endometrium whether (1) key genes involved in endometrial extracellular matrix (ECM) remodeling are regulated by the endocrine peri-ovulatory milieu; and (2) specific endometrial ECM-related transcriptome can be linked to pregnancy outcome. In Experiment 1, pre-ovulatory follicle growth of cows was manipulated to obtain two groups with specific endocrine peri-ovulatory profiles: the Large Follicle-Large CL group (LF-LCL) served as a paradigm for greater receptivity and fertility and showed greater plasma pre-ovulatory estradiol and post-ovulatory progesterone concentrations when compared to the Small Follicle-Small CL group (SF-SCL)...
October 17, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
Koki Fujimori, Toshiki Tezuka, Hiroyuki Ishiura, Jun Mitsui, Koichiro Doi, Jun Yoshimura, Hirobumi Tada, Takuya Matsumoto, Miho Isoda, Ryota Hashimoto, Nubutaka Hattori, Takuya Takahashi, Shinichi Morishita, Shoji Tsuji, Wado Akamatsu, Hideyuki Okano
Patient-specific induced pluripotent stem cells (iPSCs) facilitate understanding of the etiology of diseases, discovery of new drugs and development of novel therapeutic interventions. A frequently used starting source of cells for generating iPSCs has been dermal fibroblasts (DFs) isolated from skin biopsies. However, there are also numerous repositories containing lymphoblastoid B-cell lines (LCLs) generated from a variety of patients. To date, this rich bioresource of LCLs has been underused for generating iPSCs, and its use would greatly expand the range of targeted diseases that could be studied by using patient-specific iPSCs...
October 3, 2016: Molecular Brain
Makiko Tsutsumi, Setsuri Yokoi, Fuyuki Miya, Masafumi Miyata, Mitsuhiro Kato, Nobuhiko Okamoto, Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Shinji Saitoh, Hiroki Kurahashi
It has been well documented that variants in genes encoding centrosomal proteins cause primary autosomal recessive microcephaly, although the association between centrosomal defects and the etiology of microcephaly syndromes is not fully understood. Polo-like kinase 4 (PLK4) is one of the centrosomal proteins required for centriole duplication. We here describe a patient with microcephaly and chorioretinopathy that harbors compound heterozygous missense variants, c.[442A>G]; [2336G>A], in the PLK4 gene...
September 21, 2016: European Journal of Human Genetics: EJHG
Futian Li, John Beardall, Sinéad Collins, Kunshan Gao
Studies on the long-term responses of marine phytoplankton to ongoing ocean acidification (OA) are appearing rapidly in the literature. However, only a few of these have investigated diatoms, which is disproportionate to their contribution to global primary production. Here we show that a population of the model diatom Phaeodactylum tricornutum, after growing under elevated CO2 (1000 μatm, HCL, pHT : 7.70) for 1860 generations, showed significant differences in photosynthesis and growth from a population maintained in ambient CO2 and then transferred to elevated CO2 for 20 generations (HC)...
September 15, 2016: Global Change Biology
Junya Kitadani, Toshiyasu Ojima, Hiromitsu Iwamoto, Hiroaki Tabata, Mikihito Nakamori, Masaki Nakamura, Masahiro Katsuda, Motoki Miyazawa, Keiji Hayata, Hiroki Yamaue
The difficulty in obtaining a sufficient number of functional dendritic cells(DCs)is a well-known serious problem in DCbased immunotherapy. Therefore, we used induced pluripotent stem cell-derived DCs(iPSDCs). We have reported that mouse iPSDCs are equivalent to BMDCs, in terms of maturation and antigen presentation. In this study, the antitumor immune response of human iPSDCs expressing the carcinoembryonic antigen was examined, to determine its clinical application in gastrointestinal cancer. Human iPS cells were established from healthy human fibroblasts using a Sendai virus vector, and human iPSDCs were differentiated under a feeder-free culture...
September 2016: Gan to Kagaku Ryoho. Cancer & Chemotherapy
Nifang Niu, Tongzheng Liu, Junmei Cairns, Reynold C Ly, Xianglin Tan, Min Deng, Brooke L Fridley, Krishna R Kalari, Ryan P Abo, Gregory Jenkins, Anthony Batzler, Erin E Carlson, Poulami Barman, Sebastian Moran, Holger Heyn, Manel Esteller, Liewei Wang
Metformin is currently considered as a promising anticancer agent in addition to its anti-diabetic effect. To better individualize metformin therapy and explore novel molecular mechanisms in cancer treatment, we conducted a pharmacogenomic study using 266 lymphoblastoid cell lines (LCLs). Metformin cytotoxicity assay was performed using the MTS assay. Genome-wide association (GWA) analyses were performed in LCLs using 1.3 million SNPs, 485k DNA methylation probes, 54k mRNA expression probe sets, and metformin cytotoxicity (IC50s)...
September 11, 2016: Human Molecular Genetics
M Hackl, T Leschinger, L P Müller, K Wegmann
BACKGROUND: Chronic ligamentous instability of the elbow is an important pathology as it is accompanied with pronounced dysfunction. Moreover, it represents an established risk factor for the development of osteoarthritis. Posterolateral rotatory instability (PLRI) caused by insufficiency of the lateral collateral ligament (LCL) is the most common type of chronic elbow instability and is usually a sequel of traumatic elbow dislocation. Chronic overload can lead to insufficiency of the ulnar collateral ligament (UCL) with subsequent valgus instability, especially in overhead athletes...
October 2016: Der Orthopäde
Ling Z Morgan, Brandi Rollins, Adolfo Sequeira, William Byerley, Lynn E DeLisi, Alan F Schatzberg, Jack D Barchas, Richard M Myers, Stanley J Watson, Huda Akil, William E Bunney, Marquis P Vawter
Genome-wide association studies of schizophrenia encompassing the major histocompatibility locus (MHC) were highly significant following genome-wide correction. This broad region implicates many genes including the MHC complex class II. Within this interval we examined the expression of two MHC II genes (HLA-DPA1 and HLA-DRB1) in brain from individual subjects with schizophrenia (SZ), bipolar disorder (BD), major depressive disorder (MDD), and controls by differential gene expression methods. A third MHC II mRNA, CD74, was studied outside of the MHC II locus, as it interacts within the same immune complex...
2016: Microarrays
M Hackl, T Leschinger, C Ries, W F Neiss, L P Müller, K Wegmann
BACKGROUND: Chronic posterolateral rotatory instability (PLRI) of the elbow is the result of an insufficiency of the lateral collateral ligament (LCL). Lateral ulnar collateral ligament (LUCL) reconstruction represents a well-established treatment method for PLRI. However, recurrent instability remains a problem. OBJECTIVES: The goal of this in-vitro study was to evaluate the posterolateral rotatory stability of the intact elbow, after sectioning of the LCL and after LUCL reconstruction with a triceps tendon autograft and double BicepsButton(TM) fixation...
October 2016: Der Orthopäde
Mohammed Dany, Salih Gencer, Rose Nganga, Raquela J Thomas, Natalia Oleinik, Kyla D Baron, Zdzislaw M Szulc, Peter Ruvolo, Steven Kornblau, Michael Andreeff, Besim Ogretmen
Signaling pathways regulated by mutant Fms-like tyrosine kinase 3 (FLT3)-internal tandem duplication (ITD), which mediate resistance to acute myeloid leukemia (AML) cell death, are poorly understood. Here, we reveal that pro-cell death lipid ceramide generation is suppressed by FLT3-ITD signaling. Molecular or pharmacologic inhibition of FLT3-ITD reactivated ceramide synthesis, selectively inducing mitophagy and AML cell death. Mechanistically, FLT3-ITD targeting induced ceramide accumulation on the outer mitochondrial membrane, which then directly bound autophagy-inducing light chain 3 (LC3), involving its I35 and F52 residues, to recruit autophagosomes for execution of lethal mitophagy...
October 13, 2016: Blood
Wei-Guo Jia, Tai Zhang, Dong Xie, Qiu-Tong Xu, Shuo Ling, Qing Zhang
Seven half-sandwich cycloruthenated complexes [Ru(p-cymene)LCl] (2a-2g) (L = 2-phenyl-2-oxazoline (2a), 2-p-tolyl-4,5-dihydrooxazole (2b), 4,4-dimethyl-2-phenyl-2-oxazoline (2c), 2-(4-chlorophenyl)-4,5-dihydrooxazole (2d), 2-(4-bromophenyl)-4,5-dihydrooxazole (2e), 2-(4-fluorophenyl)-4,5-dihydrooxazole (2f) and 2-(4-nitrophenyl)-4,5-dihydrooxazole (2g)) were synthesized and characterized. All half-sandwich cycloruthenated complexes were fully characterized by (1)H and (13)C NMR spectra, elemental analyses and infrared spectroscopy...
September 28, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
Eri Nagashima, Takuya Yoshida, Satoshi Matsunaga, Kenji Nomiya
The effect of counteranions on the molecular structures of phosphanegold(i) cluster cations formed by polyoxometalate (POM)-mediated clusterization was investigated. A novel intercluster compound, [{(AuLCl)2(μ-OH)}2]3[α-PMo12O40]2·3EtOH (1-PMo12), was obtained as orange-yellow plate crystals in 12.0% yield from a 6 : 1 molar ratio reaction of the monomeric phosphanegold(i) carboxylato complex [Au(RS-pyrrld)(LCl)] (RS-Hpyrrld = RS-2-pyrrolidone-5-carboxylic acid; LCl = tris(4-chlorophenyl)phosphane) in CH2Cl2 with the free acid-form of Keggin polyoxometalate (POM), H3[α-PMo12O40]·14H2O...
September 14, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
Tessa van der Geest, Peter Laverman, Danny Gerrits, Birgitte Walgreen, Monique H Helsen, Christian Klein, Tapan K Nayak, Gert Storm, Josbert M Metselaar, Marije I Koenders, Otto C Boerman
Rheumatoid arthritis (RA) is a chronic autoimmune disorder resulting in synovial inflammation. Fibroblast activation protein (FAP) is over-expressed by fibroblast-like synoviocytes in arthritic joints. Radioimmunoimaging with anti-FAP antibodies might be used to monitor therapy response, thus enabling tailored therapy strategies and therapeutic outcomes. The aim of this study was to assess whether radiolabeled anti-FAP antibodies can be used to monitor the efficacy of treatment with prednisolone phosphate containing long-circulating liposomes (PLP-LCL) in a mouse model of arthritis...
August 4, 2016: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Jeong Woo Kim, Young Yi, Tae Kyun Kim, Hong Je Kang, Jong Yun Kim, Jong Myoung Lee, Kyu Hwan Bae, Min Su Joo
BACKGROUND: Lateral ulnar collateral ligament injury following unstable elbow dislocation can induce posterolateral rotatory instability that requires surgery. The aim of this study was to determine the effectiveness of arthroscopic repair of the lateral collateral ligament (LCL) complex in an unstable elbow joint. METHODS: The study group consisted of 13 patients who experienced posterolateral rotatory instability after an unstable elbow dislocation with an injury to the lateral ulnar collateral ligament...
August 3, 2016: Journal of Bone and Joint Surgery. American Volume
Ahmed Mohsen, Charles Saby, Philippe Collery, Gilane Mohamed Sabry, Rasha Elsherif Hassan, Abdelfattah Badawi, Pierre Jeannesson, Didier Desmaële, Hamid Morjani
Two water soluble gallium complexes described as [Ga(III)LCl], where L is the deprotonated form of N-2-hydroxybenzyl aspartic acid derivatives, were synthesized and characterized by (1)H NMR, (13)C NMR, FT-IR, mass spectrometry, and elemental analysis. The 2-(5-chloro-2-hydroxybenzylamino)succinic acid derivative (GS2) has been found to be a promising anticancer drug candidate. This compound was found to be more cytotoxic against human breast carcinoma MDA-MB231 and fibrosarcoma HT-1080 cell lines than the unsubstituted derivative and GaCl3...
October 2016: Journal of Biological Inorganic Chemistry: JBIC
Dafang Zhang, Matthew Tarabochia, Stein Janssen, David Ring, Neal Chen
OBJECTIVE: The management of terrible triad injuries has evolved over the last two decades to include routine radial head fixation or replacement, reattachment of the LCL, with or without coronoid fixation. Our objective was to determine the likelihood of and factors associated with subluxation or dislocation after operative treatment of terrible triad injuries among a large group of surgeons using current techniques. DESIGN: Retrospective cohort study SETTING:: Two Level I trauma centers PATIENTS/PARTICIPANTS:: A total of 107 patients with operatively treated terrible triad injuries from January 2000 to June 2015...
July 29, 2016: Journal of Orthopaedic Trauma
Marc S Sabatine, James A Underberg, Michael Koren, Seth J Baum
Elevation of low-density lipoprotein cholesterol (LDL-C) is an important cause of atherosclerotic cardiovascular disease (ASCVD). Over the years, clinical outcome studies with LDL-C lowering agents have revealed that reducing LCL-C levels is effective in reducing rates of major ASCVD events. Although secondary factors play a role in clinical expression, severe lipid disorders often have a strong genetic component. Genetic revelations have provided novel targets for improving LDL-C management in high-risk individuals...
October 2016: Postgraduate Medicine
Haluk Çabuk, Fatmagül Kuşku Çabuk
Proprioceptive inputs from the joints and limbs arise from mechanoreceptors in the muscles, ligaments and tendons. The knee joint has a wide range of movements, and proper neuroanatomical organization is critical for knee stability. Four ligaments (the anterior (ACL) and posterior (PCL) cruciate ligaments and the medial (MCL) and lateral (LCL) collateral ligaments) and four tendons (the semitendinosus (STT), gracilis (GT), popliteal (PoT), and patellar (PaT) tendons) from eight fresh frozen cadavers were harvested...
September 2016: Clinical Anatomy
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