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https://www.readbyqxmd.com/read/29202043/epstein-barr-virus-induces-adhesion-receptor-cd226-dnam-1-expression-during-primary-b-cell-transformation-into-lymphoblastoid-cell-lines
#1
Lisa Grossman, Chris Chang, Joanne Dai, Pavel A Nikitin, Dereje D Jima, Sandeep S Dave, Micah A Luftig
Epstein-Barr virus (EBV), an oncogenic herpesvirus, infects and transforms primary B cells into immortal lymphoblastoid cell lines (LCLs), providing a model for EBV-mediated tumorigenesis. EBV transformation stimulates robust homotypic aggregation, indicating that EBV induces molecules that mediate cell-cell adhesion. We report that EBV potently induced expression of the adhesion molecule CD226, which is not normally expressed on B cells. We found that early after infection of primary B cells, EBV promoted an increase in CD226 mRNA and protein expression...
November 2017: MSphere
https://www.readbyqxmd.com/read/29198355/editorial-commentary-knee-lateral-collateral-ligament-injury-is-more-common-than-we-thought
#2
EDITORIAL
Russell F Warren
Detection of a knee lateral collateral ligament (LCL) tear, in combination with an anterior cruciate ligament tear, may be facilitated by the heel height test, which seems a modification of Hughston's external rotation recurvatum test for posterolateral rotatory instability. The radiographic and arthroscopic stress tests are also valuable. We have found that varus stress will increase varus opening by 1° to 4° for isolated LCL tears and 5° to 9° for combined (LCL) popliteus injury; an arthroscopic stress evaluation will note a normal opening of 5 to 6 mm increasing to 9 to 10 mm in combined LCL popliteus injury...
December 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/29171950/-evolocumab-repatha%C3%A2-a-human-monoclonal-antibody-against-pcsk9-protein-as-potent-cholesterol-lowering-therapy
#3
C Wallemacq
Evolocumab is a fully human monoclonal antibody (mAb) targeting ProProtein Convertase Subtilisin/Kexin 9 (PCSK9). PCSK9 is a circulating enzyme secreted by the liver and plays a key role in the LDL-Receptors (LDL-R) turnover. Binding of PCSK9 on the extracellular part of LDL-R is responsible for its degradation in the lysosome instead of its recycling to the cell surface, thereby producing a reduction in the number of LDL-R on the cell surface, a decreased LDL-C uptake and increased levels of LDL-C. Inhibiting PCSK9 is a new way to markedly reduce LDL-C...
November 2017: Revue Médicale de Liège
https://www.readbyqxmd.com/read/29171935/single-molecule-real-time-smrt%C3%A2-dna-sequencing-of-hla-genes-at-ultra-high-resolution-from-126-international-hla-and-immunogenetics-workshop-cell-lines
#4
T R Turner, J D Hayhurst, D R Hayward, W P Bultitude, D J Barker, J Robinson, J Alejandro Madrigal, N P Mayor, S G E Marsh
The hyperpolymorphic HLA genes play important roles in disease and transplantation and act as genetic markers of migration and evolution. A panel of 107 B-lymphoblastoid cell lines (B-LCLs) was established in 1987 at the 10(th) International Histocompatibility Workshop as a resource for the immunogenetics community. These B-LCLs are well characterised and represent diverse ethnicities and HLA haplotypes. Here we have applied Pacific Biosciences' Single Molecule Real-Time (SMRT) DNA sequencing to HLA type 126 B-LCL, including the 107 IHIW cells, to ultra-high resolution...
November 24, 2017: HLA
https://www.readbyqxmd.com/read/29163031/microrna-mediated-regulation-of-itgb3-and-chl1-is-implicated-in-ssri-action
#5
Keren Oved, Luba Farberov, Avial Gilam, Ifat Israel, Danielle Haguel, David Gurwitz, Noam Shomron
Background: Selective serotonin reuptake inhibitor (SSRI) antidepressant drugs are the first-line of treatment for major depressive disorder (MDD) but are effective in <70% of patients. Our earlier genome-wide studies indicated that two genes encoding for cell adhesion proteins, close homolog of L1 (CHL1) and integrin beta-3 (ITGB3), and microRNAs, miR-151a-3p and miR-221/222, are implicated in the variable sensitivity and response of human lymphoblastoid cell lines (LCL) from unrelated individuals to SSRI drugs...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29158704/a-quality-by-design-approach-for-the-development-of-lyophilized-liposomes-with-simvastatin
#6
Alina Porfire, Dana Maria Muntean, Lucia Rus, Bianca Sylvester, Ioan Tomuţă
Lyophilization is used to ensure an increased shelf-life of liposomes, by preserving them in dry state, more stable than the aqueous dispersions. When stored as aqueous systems, the encapsulated drugs are released and the liposomes might aggregate or fuse. The aim of this study was to develop and optimize a lyophilized formulation of simvastatin (SIM) loaded into long circulating liposomes using the Quality by Design (QbD) approach. Pharmaceutical development by QbD aims to identify characteristics that are critical for the final product quality, and to establish how the critical process parameters can be varied to consistently produce a product with the desired characteristics...
November 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/29157843/predictors-of-osteoarthritis-following-operative-treatment-of-medial-tibial-plateau-fractures
#7
Markus Parkkinen, Jan Lindahl, Tatu J Mäkinen, Seppo K Koskinen, Antti Mustonen, Rami Madanat
PURPOSE: To determine factors influencing the development of posttraumatic osteoarthritis (OA) following medial tibial plateau fractures and to evaluate concomitant injuries associated with these fractures. MATERIALS AND METHODS: A chart review of patients with operatively treated medial tibial plateau fractures admitted to our Level I trauma centre from 2002 to 2008 was performed. Of 63 patients, 41 participated in a clinical and radiographic examination. The mean age was 47 years (range 16-78) and the mean follow-up time was 7...
November 14, 2017: Injury
https://www.readbyqxmd.com/read/29151419/-preparation-of-paraoxonase-liposomes-and-their-pharmacokinetics-in-rats
#8
Zhenkun Han, Suwen Jiang, Baoqin Fu, Min Zhao
OBJECTIVE: To prepare paraoxonase 1 (PON1) liposomes, and investigate pharmacokinetics of common PON1 liposomes (L-PON1) and polyethylene glycol-modified PON1 long circulating liposomes (PEG-PON1-LCL) in rats after intravenous administration. METHODS: L-PON1 and PEG-PON1-LCL were prepared by film dispersion method. The entrapment efficiency, mean diameter and Zeta potential of the liposomes were measured, and the stability was evaluated. Thirty-six Wistar rats were divided into three groups according to random number table, with 12 rats in each group...
November 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29122124/anatomical-and-radiological-evaluation-of-less-invasive-stabilisation-system-liss-in-correlation-with-knee-lateral-collateral-ligament-insertion
#9
Florian Freimoser, Stephan Grechenig, Anna Ofenhitzer, Bore Bakota, Mario Staresinic, Christian G Pfeifer
INTRODUCTION: The Less Invasive Stabilisation System (LISS) is an angle-stable plate that enables treatment of distal femoral comminuted and periprosthetic fracture. As it is placed through a minimally-invasive lateral approach, lateral knee pain is a commonly described symptom after its application. This study investigates knee lateral collateral ligament (LCL) iatrogenic injury during LISS plate fixation. A cadaver study was performed and a retrospective radiological investigation with the analysis of its clinical application was conducted to evaluate possible knee LCL damage...
November 2017: Injury
https://www.readbyqxmd.com/read/29118124/c-myc-represses-transcription-of-the-epstein-barr-virus-latent-membrane-protein-1-early-after-primary-b-cell-infection
#10
Alexander M Price, Joshua E Messinger, Micah A Luftig
Recent evidence has shown that the EBV oncogene LMP1 is not expressed at high levels early after EBV-infection of primary B cells, despite its being essential for the long-term outgrowth of immortalized lymphoblastoid cell lines (LCLs). In this study, we found that expression of LMP1 increased fifty-fold between seven days post infection and the LCL state. Metabolic labeling of nascently transcribed mRNA indicated this was primarily a transcription-mediated event. EBNA2, the key viral transcription factor regulating LMP1, and CTCF, an important chromatin insulator, were recruited to the LMP1 locus similarly early and late after infection...
November 8, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29102847/legal-cannabis-laws-home-cultivation-and-use-of-edible-cannabis-products-a-growing-relationship
#11
Jacob T Borodovsky, Alan J Budney
BACKGROUND: Over half of U.S. states have enacted legal cannabis laws (LCL). In parallel, edible cannabis products (i.e., edibles) have presented new regulatory challenges. LCL provisions that dictate access to cannabis (e.g., home cultivation (HC) or dispensaries (DSP)) may impact edible production and use. This study examined relationships among HC and DSP provisions, cannabis cultivation, and edible use. METHODS: An online cannabis use survey was distributed using Facebook...
November 2, 2017: International Journal on Drug Policy
https://www.readbyqxmd.com/read/29098869/a-step-forward-towards-the-development-of-stable-freeze-dried-liposomes-a-quality-by-design-approach-qbd
#12
Bianca Sylvester, Alina Porfire, Marcela Achim, Lucia Rus, Ioan Tomuţă
This study highlights the advantages of using a Quality by Design (QbD) approach in order to gain a more comprehensive understanding of the freeze-drying process of pravastatin-loaded long-circulating liposomes (LCL-PRAV). Within the QbD paradigm, the present study aimed to establish the design space for the optimization of freeze-dried LCL-PRAV by means of Design of Experiment (DOE). The encapsulated solute retention (ESR), the average particle size and zeta potential after freeze-drying, the residual moisture content, the macroscopic cake appearance, the glass trasition temperature (Tg) of the freeze-dried cake and the primary drying time were defined as critical quality attributes (CQAs) for the freeze-dried final product...
November 3, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/29073794/porous-titanium-wedges-in-lateral-column-lengthening-for-adult-acquired-flatfoot-deformity
#13
Spencer H Moore, S Evan Carstensen, M Tyrrell Burrus, Truitt Cooper, Joseph S Park, Venkat Perumal
Lateral column lengthening (LCL) is a common procedure for reconstruction of stage II flexible adult-acquired flatfoot deformity (AAFD). The recent development of porous titanium wedges for this procedure provides an alternative to allograft and autograft. The purpose of this study was to report radiographic and clinical outcomes achieved with porous titanium wedges in LCL. A retrospective analysis of 34 feet in 30 patients with AAFD that received porous titanium wedges for LCL from January 2011 to October 2014...
October 1, 2017: Foot & Ankle Specialist
https://www.readbyqxmd.com/read/29065040/a-cadaveric-biomechanical-analysis-of-three-lateral-ulnar-collateral-ligament-reconstructions
#14
Aakash Chauhan, Mark E Baratz, Patrick Schimoler, David Ruch, Mark Carl Miller
OBJECTIVE: To biomechanically assess the angular stability provided by three techniques for reconstruction of the LUCL. METHODS: Eight cadaveric elbows were tested with the LCL-C intact, disrupted from the origin at the lateral epicondyle, and reconstructed with three different techniques utilizing ulnar bone tunnels: a suture "lasso" or palmaris longus (PL) tendon "lasso" both docked with a metal button at the origin, and a doubled-over PL tendon docked with metal buttons at both the origin and ulnar insertion...
October 14, 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/29065035/the-effect-of-soft-tissue-injuries-on-clinical-outcomes-following-tibial-plateau-fracture-fixation
#15
Stephen J Warner, Matthew R Garner, Patrick C Schottel, Peter D Fabricant, Ryan R Thacher, Michael L Loftus, David L Helfet, Dean G Lorich
OBJECTIVE: The purpose of this study was to determine if ligamentous and meniscal injuries as determined by initial magnetic resonance imaging altered clinical outcomes following the fixation of tibial plateau fractures. DESIGN: Comparative cohort study. SETTING: Academic level I trauma center. PATIENTS/PARTICIPANTS: Eighty-two patients from a prospective database of operatively treated tibial plateau fractures met the inclusion criteria, which consisted of injury radiographs, preoperative knee MRI, and a minimum of twelve months of clinical outcomes...
October 14, 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/29052133/ebv-infection-and-glucose-metabolism-in-nasopharyngeal-carcinoma
#16
Jun Zhang, Lin Jia, Chi Man Tsang, Sai Wah Tsao
To establish persistent infection in cells, viruses evolve strategies to alter host cellular pathways to regulate cell proliferation and energy metabolism which support viral infection. Epstein-Barr virus (EBV) undergoes both lytic and latent infection to achieve persistent and lifelong infection in human. EBV readily infects human B cells, driving their transformation to proliferative lymphoblastoid cell lines (LCL), and eventually establishes lifelong latent infection in memory B cells. In contrary, EBV undergoes lytic replication upon infection into normal epithelial cells which is essential for the replication of EBV genome and production of infectious viral particles for transmission through saliva...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29029192/paracoccidioidomycosis-associated-with-a-heterozygous-stat4-mutation-and-impaired-ifn-%C3%AE-immunity
#17
Lena F Schimke, James Hibbard, Ruben Martinez-Barricarte, Taj Ali Khan, Ricardo de Souza Cavalcante, Edgar Borges de Oliveira Junior, Tabata Takahashi França, Asif Iqbal, Guilherme Yamamoto, Christina Arslanian, Claudia Feriotti, Tania Alves da Costa, Jacinta Bustamante, Stéphanie Boisson-Dupuis, Jean-Laurent Casanova, José Alexandre Marzagao Barbuto, Mayana Zatz, Rinaldo Poncio Mendes, Vera Lucia Garcia Calich, Hans D Ochs, Troy R Torgerson, Otávio Cabral-Marques, Antonio Condino-Neto
Background: Mutations in genes affecting interferon (IFN)-γ immunity have contributed to understand the essential role of IFN-γ in the protection against intracellular pathogens. However, inborn errors in STAT4, which controls IL-12 responses, have not yet been reported. Objective: To determine the underlying genetic defect in a family with a history of paracoccidioidomycosis (PCM) disease. Methods: Genetic analysis was performed by whole-exome sequencing and sanger sequencing...
October 5, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29027008/lateral-epicondylitis-associations-of-mr-imaging-and-clinical-assessments-with-treatment-options-in-patients-receiving-conservative-and-arthroscopic-managements
#18
Ji Young Jeon, Min Hee Lee, In-Ho Jeon, Hye Won Chung, Sang Hoon Lee, Myung Jin Shin
OBJECTIVES: We assessed the implications of MR imaging with clinical history in lateral epicondylitis management by evaluating imaging and clinical features in patients with lateral epicondylitis treated conservatively or operatively. METHODS: Sixty patients with lateral epicondylitis treated conservatively (n = 38) or operatively (n = 22) from 2011-2015 were included. MR imaging findings of common extensor tendon (CET), lateral collateral ligament (LCL) complex, muscle oedema, ulnar nerve and elbow joint were reviewed...
October 12, 2017: European Radiology
https://www.readbyqxmd.com/read/29024646/the-epstein-barr-virus-regulome-in-lymphoblastoid-cells
#19
Sizun Jiang, Hufeng Zhou, Jun Liang, Catherine Gerdt, Chong Wang, Liangru Ke, Stefanie C S Schmidt, Yohei Narita, Yijie Ma, Shuangqi Wang, Tyler Colson, Benjamin Gewurz, Guoliang Li, Elliott Kieff, Bo Zhao
Epstein-Barr virus (EBV) transforms B cells to continuously proliferating lymphoblastoid cell lines (LCLs), which represent an experimental model for EBV-associated cancers. EBV nuclear antigens (EBNAs) and LMP1 are EBV transcriptional regulators that are essential for LCL establishment, proliferation, and survival. Starting with the 3D genome organization map of LCL, we constructed a comprehensive EBV regulome encompassing 1,992 viral/cellular genes and enhancers. Approximately 30% of genes essential for LCL growth were linked to EBV enhancers...
October 11, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28977893/combination-of-betulinic-acid-and-chidamide-synergistically-inhibits-epstein-barr-virus-replication-through-over-generation-of-reactive-oxygen-species
#20
Haibing Yu, Hongyu Zhang, Zhigang Chu, Qiongfang Ruan, Xueru Chen, Danli Kong, Xiaodong Huang, Huawen Li, Huanwen Tang, Hongjin Wu, Yifei Wang, Weiguo Xie, Yuanling Ding, Paul Yao
Epstein-Barr virus (EBV) has widely infected more than 90% of human populations. Currently, there is no efficient way to remove the virus because the EBV carriers are usually in a latent stage that allows them to escape the immune system and common antiviral drugs. In the effort to develop an efficient strategy for the removal of the EBV virus, we have shown that betulinic acid (BA) slightly suppresses EBV replication through SOD2 suppression with subsequent reactive oxygen species (ROS) generation and DNA damage in EBV-transformed LCL (lymphoblastoid cell line) cells...
September 22, 2017: Oncotarget
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