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skin test concentration

Frederik Flenner, Nicole Arlt, Mahtab Nasib, Sophie Schobesberger, Thea Koch, Ursula Ravens, Felix Friedrich, Viacheslav Nikolaev, Torsten Christ, Sebastian N Stehr
BACKGROUND: Systemic toxicity of local anesthetics is predominantly complicated by their myocardial toxicity. Especially long-acting local anesthetics exert a negative inotropic effect that has been described at lower concentrations than defined for blockade of myocardial ion channels. We evaluated the negative inotropic effect of bupivacaine at a concentration described for clinical toxicity testing the hypothesis that negative inotropy is a result of reduced Ca sensitivity rather than blockade of ion channels...
March 16, 2018: Anesthesiology
Edgar Isaac Rojas-Bedolla, Jorge Luis Gutiérrez-Pérez, Mario Iván Arenas-López, Marco Martin González-Chávez, Juan Ramón Zapata-Morales, Claudia Leticia Mendoza-Macías, Candy Carranza-Álvarez, Juan José Maldonado-Miranda, Martha Alicia Deveze-Álvarez, Angel Josabad Alonso-Castro
ETHNOPHARMACOLOGICAL RELEVANCE: Celtis pallida Torr (Cannabaceae) is employed as a folk medicine for the treatment of inflammation, pain, skin infections, and diarrhea, among other diseases. AIM OF THE STUDY: The purpose of this work was to assess the chemical composition, the in vitro and in vivo toxicity, the antimicrobial, anti-inflammatory, antidiarrheal, antinociceptive, locomotor, and sedative effects of an ethanolic extract obtained from Celtis pallida aerial parts (CPE)...
March 12, 2018: Journal of Ethnopharmacology
C Ling, Q Sun, J Khang, M Felipa Lastarria, J Strong, B Stolze, X Yu, T P Parikh, M A Waldman, K Welsh, J Jonklaas, L Masika, S J Soldin
Objectives: To evaluate the reliability of normal Thyroid Stimulating Hormone (TSH) as a thyroid function test and assess the effect of Adrenocorticotropic Hormone (ACTH) on serum TSH concentration. Design and Methods: Patients presenting to the National Institutes of Health Department of Endocrinology outpatient clinic with symptoms consistent with hypothyroidism were identified. Thyroid hormone concentrations were measured by liquid chromatography/tandem mass spectrometry and immunoassay...
2018: Annals of Thyroid Research
M Fiorentzis, H Kalirai, P Katopodis, B Seitz, A Viestenz, S E Coupland
Electrochemotherapy (ECT) enhances responsiveness to cytotoxic drugs in numerous cell lines in vitro. Clinically ECT is widely applied for skin tumor ablation and has shown efficacy in treating non-resectable colorectal liver metastases. There is limited experience of ECT for ocular tumor therapy. We investigated the cytotoxic effect of bleomycin and cisplatin in combination with electroporation on chemoresistant human uveal melanoma (UM) cell lines in vitro. Four UM cell lines (Mel 270, 92-1, OMM-1, OMM-2...
2018: Neoplasma
Rita Palmeira-de-Oliveira, Rita Monteiro Machado, José Martinez-de-Oliveira, Ana Palmeira-de-Oliveira
The HET-CAM (Hen's Egg Test-Chorioallantoic Membrane) assay is an in vitro alternative to the in vivo Draize Rabbit Eye test that mimics vascular changes in the chorioallantoic membrane. This qualitative method assesses the irritancy potential of chemicals. The CAM responds to injury with an inflammatory process similar to that in the rabbit eye's conjunctival tissue. Regarding topical toxicity assessment of medical devices, ISO 10993-10 states that any skin or eye irritant material shall be directly labelled as a potential vaginal irritant without animal testing, suggesting that the irritation potential for the eye and the vaginal epithelia is similar...
March 11, 2018: ALTEX
Gautam Achar, C R Shahini, Siddappa A Patil, Jan Grzegorz Małecki, Szu-Hua Pan, Albert Lan, Xuan-Ren Chen, Srinivasa Budagumpi
In this contribution, a series of sterically-encumbered coumarin substituted benzimidazole-based N-heterocyclic carbene (NHC) precursors (1-12) and their silver(I)-NHC complexes (13-24) are reported. Molecular structure of NHC precursors 8 and 12 and cationic complexes 15 and 16 was established by single crystal X-ray diffraction method. The silver(I) complexes demonstrated various significant intramolecular agostic-like interactions operating between the metal center and the hydrogen atoms of the substituents alongside a variety of feeble π-π stacking interactions...
February 22, 2018: Journal of Inorganic Biochemistry
Katarzyna Miranowicz-Dzierżawska
The aim of this study was to assess whether there are differences between the results of determining oxidative stress markers obtained from different origin cell lines after exposure to chemicals generating free radicals. The studies considered two markers of oxidative stress: the level of thiobarbituric acid reactive substances (TBARS) and superoxide dismutase activity. The evaluation was performed in five cell lines: Chinese hamster ovary (CHO-9) cells, lung adenocarcinoma A549, macrophages RAW264.7, skin carcinoma cells A431, and keratinocytes HaCaT...
January 1, 2018: Toxicology and Industrial Health
Takeshi Matsubara, Hiroshi Iwamoto, Yuki Nakazato, Tomoyuki Okamoto, Tatsuya Ehara, Hirohisa Izumi, Yasuhiro Takeda
BACKGROUND: Epicutaneous sensitization to food allergens can occur through defective skin barriers. However, the relationship between oral tolerance and epicutaneous sensitization remains to be elucidated. We aimed to determine whether prior oral exposure to whey proteins or their hydrolysates prevents epicutaneous sensitization and subsequent food allergic reaction to the whey protein, β-lactoglobulin (β-LG), and investigated the underlying mechanisms. METHODS: BALB/c mice were given whey protein concentrate (WPC), two kinds of partial whey protein hydrolysate (PWH1 or PWH2), or extensive whey protein hydrolysate (EWH) in drinking water for 21 days...
March 8, 2018: Pediatric Allergy and Immunology
Christopher Inglefield, Ulf Emanuel Samuelson, Marina Landau, Dale DeVore
Background: Despite the increasing popularity of facial contouring with hyaluronic acid, innovation has been limited to iterations that feature different particle sizes, concentrations and degrees of crosslinking. Bio-dermal restoration is a new approach for correcting facial tissue defects by supplementing the natural dermal structure providing a scaffold for fibroblast adherence and proliferation. Objective: The purpose of the study was to evaluate the safety of RPC Pure-Collagen for the treatment of facial contours...
March 2, 2018: Aesthetic Surgery Journal
Daniela Benedec, Ilioara Oniga, Flavia Cuibus, Bogdan Sevastre, Gabriela Stiufiuc, Mihaela Duma, Daniela Hanganu, Cristian Iacovita, Rares Stiufiuc, Constantin Mihai Lucaciu
Purpose: The leaves and flowering stem of Origanum vulgare contain essential oils, flavonoids, phenolic acids and anthocyanins. We propose a new, simple, one-pot, O. vulgare extract (OVE) mediated green synthesis method of biocompatible gold nanoparticles (AuNPs) possessing improved antioxidant, antimicrobial and plasmonic properties. Materials and methods: Different concentrations of OVEs were used to reduce gold ions and to synthetize biocompatible spherical AuNPs...
2018: International Journal of Nanomedicine
Yanyan Liu, Okechukwu C Ndumnego, Tingting Chen, Ryung S Kim, Elizabeth R Jenny-Avital, Thumbi Ndung'u, Douglas Wilson, Jacqueline M Achkar
Sputum smear-negative HIV-associated active tuberculosis (TB) is challenging to diagnose. CD14 is a pattern recognition receptor that is known to mediate monocyte activation. Prior studies have shown increased levels of soluble CD14 (sCD14) as a potential biomarker for TB, but little is known about its value in detecting smear-negative HIV-associated TB. We optimized a sandwich ELISA for the detection of sCD14, and tested sera from 56 smear-negative South African (39 culture-positive and 17 culture-negative) HIV-infected pulmonary TB patients and 24 South African and 43 US (21 positive and 22 negative for tuberculin skin test, respectively) HIV-infected controls...
February 27, 2018: Pathogens
Gabriel Marrero-Alemán, Pedro Saavedra Santana, Federica Liuti, Noelia Hernández, Esmeralda López-Jiménez, Leopoldo Borrego
BACKGROUND: Sensitivity to methylchloroisothiazolinone (MCI)/methylisothiazolinone (MI) has increased rapidly over recent years. This increase is mainly related to the extensive use of high concentrations of MI in cosmetic products, although a growing number of cases of occupational allergic contact dermatitis are caused by MCI/MI. OBJECTIVE: The aim of this study was to examine the association between the increase in MCI/MI sensitization and the work undertaken by the patients in our area...
February 28, 2018: Dermatitis
Sabrina Dahlizar, Mika Futaki, Akie Okada, Wesam Radhi Kadhum, Hiroaki Todo, Kenji Sugibayashi
Palmitoyl-glycine-histidine (Pal-GH) is a new low molecular weight gelling agent. It exhibits thixotropic behavior, low viscosity, and high dissolving properties for a wide range of hydrophilic to lipophilic drugs. Orally administered ivermectin (IVM) is used to treat scabies. However, this treatment is associated with well-known side effects, thus a study is awaited to search for alternative routes of administration. Although a topical formulation of IVM could be a candidate, it requires whole body application except the head and face for several hours on a daily basis...
2018: Chemical & Pharmaceutical Bulletin
Nicola Zerbinati, Torello Lotti, Damiano Monticelli, Raffaele Rauso, Pablo González-Isaza, Edoardo D'Este, Alberto Calligaro, Sabrina Sommatis, Cristina Maccario, Roberto Mocchi, Jacopo Lotti, Uwe Wollina, Georgi Tchernev, Katlein França
OBJECTIVE: Neauvia Stimulate is biocompatible, injectable hyaluronic acid (HA) filler (26 mg/ml) PEG cross-linked with 1% of calcium hydroxyapatite (CaHA) for facial soft-tissue augmentation that provides volume to tissues, followed by process of neocollagenesis for improving skin quality. AIM: The aim of the present study is to evaluate the biosafety of the product (Lot. 160517-26-1/2 PEG) on human keratinocytes cultured in vitro. MATERIAL AND METHODS: The experimental model proposed, despite being an in vitro system, allows the derivation of useful information to predict the possible activity of the product in further in vivo application...
January 25, 2018: Open Access Macedonian Journal of Medical Sciences
M Al Ahmad, N Arifhodzic, J Nurkic, E Jusufovic, A L Hanoun, T Rodriguez
Introduction. Sensitization to cat allergens is common worldwide. Currently, there is a trend towards costly and often unavailable diagnostic analysis. Objectives. The aim is to assess the reliability of skin prick test (SPT) and serum specific IgE (ssIgE) to cat sensitization, by performing nasal challenge test (NCT) in a community with low cat ownership but common presence of stray cats. Patients and methods. Forty-one pa-tients with perennial allergic rhinitis (AR) who were mono or polysensitized (including cat) were included...
January 17, 2018: European Annals of Allergy and Clinical Immunology
Megan Verdon, R S Morrison, P H Hemsworth
This experiment examined the effects of group composition on sow aggressive behaviour and welfare. Over 6 time replicates, 360 sows (parity 1-6) were mixed into groups (10 sows per pen, 1.8 m2 /sow) composed of animals that were predicted to be aggressive (n = 18 pens) or groups composed of animals that were randomly selected (n = 18 pens). Aggressive sows were selected based on a model-pig test that has been shown to be related to the aggressive behaviour of parity 2 sows when subsequently mixed in groups...
February 20, 2018: Behavioural Processes
A M Buenfil-Rojas, T Alvarez-Legorreta, J R Cedeño-Vázquez
Even though studies of heavy metals and their relation with metallothioneins (MTs) in reptile tissues have been conducted, research on heavy metals and MTs in organs and blood fractions of crocodylians is limited. To date there are no studies on the distribution of MTs in organs and their relation with mercury (Hg), or the concentration of MTs in plasma and erythrocytes of crocodylians. In order to understand the role of MTs in crocodiles, our aim was to assess the detoxification mechanisms for exposure to metals in Morelet's crocodile (Crocodylus moreletii) by using two biomarkers (Hg and MTs) in blood fractions and tissues, and comparing them with concentrations between two populations of crocodiles, one previously wild and currently captive (Theme Park) and another raised in a Wildlife Management Unit (WMU)...
February 14, 2018: Chemosphere
Pierre Delpech, Muna ALeryan, Brian Jones, Curtis Gemmell, Sue Lang
Skin and soft tissue infections (SSTI) are among the most commonly occurring infections and evidence suggests that these are increasing world-wide. The aetiology is diverse, but Staphylococcus aureus predominate and these are often resistant to antimicrobials that were previously effective. Tedizolid is a new oxazolidinone-class antibacterial indicated for the treatment of adults with SSTI caused by Gram-positive pathogens, including S. aureus. The aim of this study was to evaluate the in vitro efficacy of tedizolid in comparison to other clinically used antibacterials against antibiotic sensitive- and resistant-staphylococci, grown in planktonic cultures and as biofilms reflecting the growth of the microorganism during episodes of SSTI...
January 10, 2018: Diagnostic Microbiology and Infectious Disease
Ernst M Bomhard
Indium oxide (In 2 O 3 ) is a technologically important semiconductor essentially used, doped with tin oxide, to form indium tin oxide (ITO). It is poorly soluble in all so far tested physiologic media. After repeated inhalation, In 2 O 3 particles accumulate in the lungs. Their mobilization can cause significant systemic exposure over long periods of time. An increasing number of cases of severe lung effects (characterized by pulmonary alveolar proteinosis, emphysema and/or interstitial fibrosis) in workers of the ITO industry warrants a review of the toxicological hazards also of In 2 O 3 ...
February 7, 2018: Environmental Toxicology and Pharmacology
Devangsingh Sankhala, Sriram Muthukumar, Shalini Prasad
A four-channel electrochemical impedance spectroscopy (EIS) analyzer module has been demonstrated on flexible chemi-impedance sensors fabricated with gold electrodes for wearable applications. The module can perform time division multiplexed (TDM) impedance measurements on four sensors at 1 kHz. In this work, we characterize the system for the detection of cortisol in an ultralow volume (1-3 µL) of perspired human sweat, sensor performance, and effects during continuous cortisol dosing and with pH and temperature variations expected on the surface of the skin that would be representative of use conditions as seen by a wearable device...
February 1, 2018: SLAS Technology
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