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Brown adipose oxygen consumption

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https://www.readbyqxmd.com/read/29106496/transplantation-of-brown-adipose-tissue-inhibits-atherosclerosis-in-apoe-mice-contribution-of-the-activated-fgf-21-adiponectin-axis
#1
Masakazu Kikai, Hiroyuki Yamada, Noriyuki Wakana, Kensuke Terada, Keita Yamamoto, Naotoshi Wada, Shinichiro Motoyama, Makoto Saburi, Takeshi Sugimoto, Daisuke Irie, Taku Kato, Hiroyuki Kawahito, Takehiro Ogata, Satoaki Matoba
Aims: Brown adipose tissue (BAT) has been identified as an endocrine organ that maintains metabolic homeostasis; however, the effects on atherosclerosis remain undefined. Here, we investigated the effect of experimental BAT transplantation on atherosclerosis. Methods and Results: Interscapular BAT was dissected from 12-week-old wild-type mice and transplanted into the visceral cavity of 12-week-old apoE-/- mice. Oil-red O staining of whole aortas after 3 months of a high-cholesterol diet showed a significant decrease in atherosclerotic lesion area in BAT-transplanted mice by 20% compared with the sham control mice...
November 2, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/29077919/glucocorticoids-suppressing-the-browning-of-adipose-tissue-via-mir-19b-in-male-mice
#2
Yifan Lv, Jing Yu, Yunlu Sheng, Min Huang, Xiaocen Kong, Wenjuan Di, Juan Liu, Hong Zhou, Hui Liang, Guoxian Ding
Physiological levels of glucocorticoids are required for proper metabolic control, and excessive glucocorticoid action has been linked to a variety of pandemic metabolic diseases. microRNA (miRNA)-19b plays a critical role in the pathogenesis of glucocorticoid-induced metabolic diseases. This study sought to explore the potential of miRNA-based therapeutics targeting adipose tissue. Our results showed that overexpressed miR-19b in stromal vascular fraction cells derived from subcutaneous adipose tissue had the same effects as dexamethasone treatment on the inhibition of adipose browning and oxygen consumption rate...
October 24, 2017: Endocrinology
https://www.readbyqxmd.com/read/29064586/a-pivotal-role-of-ampk-signaling-in-medicarpin-mediated-formation-of-brown-and-beige-adipocytes-from-c3h10t1-2-mesenchymal-stem-cells
#3
Khan Mohammad Imran, Dahyeon Yoon, Yong-Sik Kim
Obesity poses a substantial threat of a worldwide epidemic and requires better understanding of adipose-tissue biology as well as necessitates research into the etiology and therapeutic interventions. In this study, Medicarpin (Med), a natural pterocarpan, was selected (by screening) as a small-molecule inducer of adipocyte differentiation among 854 candidates by using C3H10T1/2 mesenchymal stem cell; a cellular model of adipogenesis. Med induced the expression of brown-adipocyte commitment marker Bmp7 as well as the early regulators of brown fat fate Pparγ, Prdm16, and Pgc-1α during differentiation of C3H10T1/2 mesenchymal stem cells...
October 24, 2017: BioFactors
https://www.readbyqxmd.com/read/28970184/the-ppar%C3%AE-agonist-rosiglitazone-promotes-the-induction-of-brite-adipocytes-increasing-%C3%AE-adrenoceptor-mediated-mitochondrial-function-and-glucose-uptake
#4
Jon Merlin, Masaaki Sato, Cameron Nowell, Mohsen Pakzad, Richard Fahey, Jie Gao, Nodi Dehvari, Roger J Summers, Tore Bengtsson, Bronwyn A Evans, Dana S Hutchinson
Recruitment and activation of brite (or beige) adipocytes has been advocated as a potential avenue for manipulating whole-body energy expenditure. Despite numerous studies illustrating the differences in gene and protein markers between brown, brite and white adipocytes, there is very little information on the adrenergic regulation and function of these brite adipocytes. We have compared the functional (cyclic AMP accumulation, oxygen consumption rates, mitochondrial function, glucose uptake, extracellular acidification rates, calcium influx) profiles of mouse adipocytes cultured from three contrasting depots, namely interscapular brown adipose tissue, and inguinal or epididymal white adipose tissues, following chronic treatment with the peroxisome proliferator-activated receptor γ (PPARγ) agonist rosiglitazone...
September 29, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28957413/characterization-of-immortalized-human-brown-and-white-pre-adipocyte-cell-models-from-a-single-donor
#5
Lasse K Markussen, Marie S Isidor, Peter Breining, Elise S Andersen, Nanna E Rasmussen, Louise I Petersen, Steen B Pedersen, Bjørn Richelsen, Jacob B Hansen
Brown adipose tissue with its constituent brown adipocytes is a promising therapeutic target in metabolic disorders due to its ability to dissipate energy and improve systemic insulin sensitivity and glucose homeostasis. The molecular control of brown adipocyte differentiation and function has been extensively studied in mice, but relatively little is known about such regulatory mechanisms in humans, which in part is due to lack of human brown adipose tissue derived cell models. Here, we used retrovirus-mediated overexpression to stably integrate human telomerase reverse transcriptase (TERT) into stromal-vascular cell fractions from deep and superficial human neck adipose tissue biopsies from the same donor...
2017: PloS One
https://www.readbyqxmd.com/read/28842503/the-c-terminal-fibrinogen-like-domain-of-angiopoietin-like-4-stimulates-adipose-tissue-lipolysis-and-promotes-energy-expenditure
#6
Allison E McQueen, Deepthi Kanamaluru, Kimberly Yan, Nora E Gray, Leslie Wu, Mei-Lan Li, Anthony Chang, Adeeba Hasan, Daniel Stifler, Suneil K Koliwad, Jen-Chywan Wang
Angptl4 (Angiopoietin-like 4) is a circulating protein secreted by white and brown adipose tissues and the liver. Structurally, Angptl4 contains an N-terminal coiled-coil domain (CCD) connected to a C-terminal fibrinogen-like domain (FLD) via a cleavable linker, and both full-length Angptl4 and its individual domains circulate in the bloodstream. Angptl4 inhibits extracellular lipoprotein lipase (LPL) activity and stimulates the lipolysis of triacylglycerol stored by adipocytes in the white adipose tissue (WAT)...
September 29, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28751675/cell-cycle-arrest-in-mature-adipocytes-impairs-bat-development-but-not-wat-browning-and-reduces-adaptive-thermogenesis-in-mice
#7
Yuko Okamatsu-Ogura, Keigo Fukano, Ayumi Tsubota, Junko Nio-Kobayashi, Kyoko Nakamura, Masami Morimatsu, Hiroshi Sakaue, Masayuki Saito, Kazuhiro Kimura
We previously reported brown adipocytes can proliferate even after differentiation. To test the involvement of mature adipocyte proliferation in cell number control in fat tissue, we generated transgenic (Tg) mice over-expressing cell-cycle inhibitory protein p27 specifically in adipocytes, using the aP2 promoter. While there was no apparent difference in white adipose tissue (WAT) between wild-type (WT) and Tg mice, the amount of brown adipose tissue (BAT) was much smaller in Tg mice. Although BAT showed a normal cellular morphology, Tg mice had lower content of uncoupling protein 1 (UCP1) as a whole, and attenuated cold exposure- or β3-adrenergic receptor (AR) agonist-induced thermogenesis, with a decrease in the number of mature brown adipocytes expressing proliferation markers...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28723567/cold-inducible-sirt6-regulates-thermogenesis-of-brown-and-beige-fat
#8
Lu Yao, Xiaona Cui, Qi Chen, Xiaoying Yang, Fude Fang, Jun Zhang, Guoqing Liu, Wanzhu Jin, Yongsheng Chang
Promoting development and function of brown and beige fat may reduce obesity. Here, we show that fat SIRT6 expression is markedly induced by cold exposure and a β-adrenergic agonist. Deletion of SIRT6 in adipose tissue impairs the thermogenic function of brown adipocytes, causing a morphological "whitening" of brown fat, reduced oxygen (O2) consumption, obesity, decreased core body temperature, and cold sensitivity. Fat SIRT6-deleted mice exhibit increased blood glucose levels, severe insulin resistance, and hepatic steatosis...
July 18, 2017: Cell Reports
https://www.readbyqxmd.com/read/28717127/experimental-evidence-reveals-the-ucp1-genotype-changes-the-oxygen-consumption-attributed-to-non-shivering-thermogenesis-in-humans
#9
Takayuki Nishimura, Takafumi Katsumura, Midori Motoi, Hiroki Oota, Shigeki Watanuki
Humans have spread out all over the world adapting to many different cold environments. Recent worldwide genome analyses and animal experiments have reported dozens of genes associated with cold adaptation. The uncoupling protein 1 (UCP1) gene enhances thermogenesis reaction in a physiological process by blocking ATP (adenosine triphosphate) synthesis on a mitochondrial membrane in brown adipose tissues. To our knowledge, no previous studies have shown an association between variants of the UCP1 gene and physiological phenotypes concerning non-shivering thermogenesis (NST) under the condition of low temperature in humans...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28642596/sgbs-cells-as-a-model-of-human-adipocyte-browning-a-comprehensive-comparative-study-with-primary-human-white-subcutaneous-adipocytes
#10
Chia Rou Yeo, Madhur Agrawal, Shawn Hoon, Asim Shabbir, Manu Kunaal Shrivastava, Shiqi Huang, Chin Meng Khoo, Vanna Chhay, M Shabeer Yassin, E Shyong Tai, Antonio Vidal-Puig, Sue-Anne Toh
The Simpson Golabi Behmel Syndrome (SGBS) pre-adipocyte cell strain is widely considered to be a representative in vitro model of human white pre-adipocytes. A recent study suggested that SGBS adipocytes exhibit an unexpected transient brown phenotype. Here, we comprehensively examined key differences between SGBS adipocytes and primary human white subcutaneous (PHWSC) adipocytes. RNA-Seq analysis revealed that extracellular matrix (ECM)-receptor interaction and metabolic pathways were the top two KEGG pathways significantly enriched in SGBS adipocytes, which included positively enriched mitochondrial respiration and oxidation pathways...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28642579/regulation-of-glycolysis-in-brown-adipocytes-by-hif-1%C3%AE
#11
Astrid L Basse, Marie S Isidor, Sally Winther, Nina B Skjoldborg, Maria Murholm, Elise S Andersen, Steen B Pedersen, Christian Wolfrum, Bjørn Quistorff, Jacob B Hansen
Brown adipose tissue takes up large amounts of glucose during cold exposure in mice and humans. Here we report an induction of glucose transporter 1 expression and increased expression of several glycolytic enzymes in brown adipose tissue from cold-exposed mice. Accordingly, these genes were also induced after β-adrenergic activation of cultured brown adipocytes, concomitant with accumulation of hypoxia inducible factor-1α (HIF-1α) protein levels. HIF-1α accumulation was dependent on uncoupling protein 1 and generation of mitochondrial reactive oxygen species...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28469146/optical-visualisation-of-thermogenesis-in-stimulated-single-cell-brown-adipocytes
#12
Rókus Kriszt, Satoshi Arai, Hideki Itoh, Michelle H Lee, Anna G Goralczyk, Xiu Min Ang, Aaron M Cypess, Andrew P White, Farnaz Shamsi, Ruidan Xue, Jung Yeol Lee, Sung-Chan Lee, Yanyan Hou, Tetsuya Kitaguchi, Thankiah Sudhaharan, Shin'ichi Ishiwata, E Birgitte Lane, Young-Tae Chang, Yu-Hua Tseng, Madoka Suzuki, Michael Raghunath
The identification of brown adipose deposits in adults has led to significant interest in targeting this metabolically active tissue for treatment of obesity and diabetes. Improved methods for the direct measurement of heat production as the signature function of brown adipocytes (BAs), particularly at the single cell level, would be of substantial benefit to these ongoing efforts. Here, we report the first application of a small molecule-type thermosensitive fluorescent dye, ERthermAC, to monitor thermogenesis in BAs derived from murine brown fat precursors and in human brown fat cells differentiated from human neck brown preadipocytes...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28435771/brown-adipose-tissue-bioenergetics-a-new-methodological-approach
#13
María Calderon-Dominguez, Martín Alcalá, David Sebastián, Antonio Zorzano, Marta Viana, Dolors Serra, Laura Herrero
The rediscovery of brown adipose tissue (BAT) in humans and its capacity to oxidize fat and dissipate energy as heat has put the spotlight on its potential as a therapeutic target in the treatment of several metabolic conditions including obesity and diabetes. To date the measurement of bioenergetics parameters has required the use of cultured cells or extracted mitochondria with the corresponding loss of information in the tissue context. Herein, we present a method to quantify mitochondrial bioenergetics directly in BAT...
April 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/28322447/regulation-role-of-crtc3-in-skeletal-muscle-and-adipose-tissue
#14
Jiaqi Liu, Ziye Xu, Weiche Wu, Yizhen Wang, Tizhong Shan
The cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) signaling pathway plays important role in regulating energy homeostasis. Many of the effects of the cAMP-PKA signaling is mediated through the cAMP responsive element binding protein (CREB) and its coactivator CREB-regulated transcription coactivators (CRTCs). CRTC3 is a member of CRTCs family proteins and plays important roles in glucose and energy metabolism. Previous studies show that global knockout of CRTC3 enhances oxygen consumption and energy expenditure and subsequently protects the knockout animal against obesity...
March 21, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28288167/erythropoietin-epo-ameliorates-obesity-and-glucose-homeostasis-by-promoting-thermogenesis-and-endocrine-function-of-classical-brown-adipose-tissue-bat-in-diet-induced-obese-mice
#15
Kazuki Kodo, Satoru Sugimoto, Hisakazu Nakajima, Jun Mori, Ikuyo Itoh, Shota Fukuhara, Keiichi Shigehara, Taichiro Nishikawa, Kitaro Kosaka, Hajime Hosoi
Erythropoietin (EPO), clinically used as a hematopoietic drug, has received much attention due to its nonhematopoietic effects. EPO reportedly has beneficial effects on obesity and diabetes mellitus. We investigated whether interscapular brown adipose tissue (iBAT: main part of classical BAT) could play a role in EPO's anti-obesity and anti-diabetic effects in diet-induced obese mice. Four-week-old male C57BL/6J mice were fed a high-fat diet (HFD-Con), and half were additionally given an intraperitoneal injection of recombinant human EPO (200 IU/kg) (HFD-EPO) thrice a week for four weeks...
2017: PloS One
https://www.readbyqxmd.com/read/28276197/phenyl-%C3%AE-valerolactones-flavan-3-ol-colonic-metabolites-protect-brown-adipocytes-from-oxidative-stress-without-affecting-their-differentiation-or-function
#16
Laura Mele, Stefania Carobbio, Nicoletta Brindani, Claudio Curti, Sergio Rodriguez-Cuenca, Guillaume Bidault, Pedro Mena, Ilaria Zanotti, Michele Vacca, Antonio Vidal-Puig, Daniele Del Rio
SCOPE: Consumption of products rich in flavan-3-ols, such as tea and cocoa, has been associated with decreased obesity, partially dependent on their capacity to enhance energy expenditure. Despite these phenolics having been reported to increase the thermogenic program in brown and white adipose tissue, flavan-3-ols are vastly metabolised in vivo to phenyl-γ-valerolactones. Therefore, we hypothesize that phenyl-γ-valerolactones may directly stimulate the differentiation and the activation of brown adipocytes...
March 9, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28123939/global-ip6k1-deletion-enhances-temperature-modulated-energy-expenditure-which-reduces-carbohydrate-and-fat-induced-weight-gain
#17
Qingzhang Zhu, Sarbani Ghoshal, Richa Tyagi, Anutosh Chakraborty
OBJECTIVE: IP6 kinases (IP6Ks) regulate cell metabolism and survival. Mice with global (IP6K1-KO) or adipocyte-specific (AdKO) deletion of IP6K1 are protected from diet induced obesity (DIO) at ambient (23 °C) temperature. AdKO mice are lean primarily due to increased AMPK mediated thermogenic energy expenditure (EE). Thus, at thermoneutral (30 °C) temperature, high fat diet (HFD)-fed AdKO mice expend energy and gain body weight, similar to control mice. IP6K1 is ubiquitously expressed; thus, it is critical to determine to what extent the lean phenotype of global IP6K1-KO mice depends on environmental temperature...
January 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/28089567/adipose-tissue-clk2-promotes-energy-expenditure-during-high-fat-diet-intermittent-fasting
#18
Maximilian Hatting, Amy K Rines, Chi Luo, Mitsuhisa Tabata, Kfir Sharabi, Jessica A Hall, Francisco Verdeguer, Christian Trautwein, Pere Puigserver
A promising approach to treating obesity is to increase diet-induced thermogenesis in brown adipose tissue (BAT), but the regulation of this process remains unclear. Here we find that CDC-like kinase 2 (CLK2) is expressed in BAT and upregulated upon refeeding. Mice lacking CLK2 in adipose tissue exhibit exacerbated obesity and decreased energy expenditure during high-fat diet intermittent fasting. Additionally, tissue oxygen consumption and protein levels of UCP1 are reduced in CLK2-deficient BAT. Phosphorylation of CREB, a transcriptional activator of UCP1, is markedly decreased in BAT cells lacking CLK2 due to enhanced CREB dephosphorylation...
February 7, 2017: Cell Metabolism
https://www.readbyqxmd.com/read/28073315/metabolically-active-three-dimensional-brown-adipose-tissue-engineered-from-white-adipose-derived-stem-cells
#19
Jessica P Yang, Amy E Anderson, Annemarie McCartney, Xavier Ory, Garret Ma, Elisa Pappalardo, Joel Bader, Jennifer H Elisseeff
Brown adipose tissue (BAT) has a unique capacity to expend calories by decoupling energy expenditure from ATP production, therefore BAT could realize therapeutic potential to treat metabolic diseases such as obesity and type 2 diabetes. Recent studies have investigated markers and function of native BAT, however, successful therapies will rely on methods that supplement the small existing pool of brown adipocytes in adult humans. In this study, we engineered BAT from both human and rat adipose precursors and determined whether these ex vivo constructs could mimic in vivo tissue form and metabolic function...
April 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28025824/four-week-cold-acclimation-in-adult-humans-shifts-uncoupling-thermogenesis-from-skeletal-muscles-to-brown-adipose-tissue
#20
Denis P Blondin, Amani Daoud, Taryn Taylor, Hans C Tingelstad, Véronic Bézaire, Denis Richard, André C Carpentier, Albert W Taylor, Mary-Ellen Harper, Céline Aguer, François Haman
KEY POINTS: Muscle-derived thermogenesis during acute cold exposure in humans consists of a combination of cold-induced increases in skeletal muscle proton leak and shivering. Daily cold exposure results in an increase in brown adipose tissue oxidative capacity coupled with a decrease in the cold-induced skeletal muscle proton leak and shivering intensity. Improved coupling between electromyography-determined muscle activity and whole-body heat production following cold acclimation suggests a maintenance of ATPase-dependent thermogenesis and decrease in skeletal muscle ATPase independent thermogenesis...
March 15, 2017: Journal of Physiology
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