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Stephanie N Kinney, Jayanna Slayten, Dan A Waxman
BACKGROUND: Within transfusion medicine, the education of molecular technologies lacks standardization. OBJECTIVE: To address this problem, we surveyed specialist in blood bank technology (SBBT) programs, immunohematology reference laboratories, and SBBT graduates to define its current state. METHODS: An anonymous online survey (SurveyMonkey) was emailed to the 15 American Association of Blood Banks (AABB) SBBT programs, 59 AABB IRLs, and 82 SBBT graduates...
November 2016: Laboratory Medicine
Paola Ester López-Díaz, María Del Rocío Ruiz-Olivera, Luis Alberto Hernández-Osorio, Jaime Vargas-Arzola, Xareni Valle-Jiménez, Sergio Roberto Aguilar-Ruiz, Honorio Torres-Aguilar
Irregular antibodies are produced by alloimmunization because of pregnancies or blood transfusions. They are called "irregular" due to target erythrocyte antigens from "rare blood systems," those different from the ABO system. Irregular antibodies have been widely investigated in immunohematology since their presence in blood donors may lead to difficulties in blood typing and in blood cross-matching, or to induce hemolytic transfusion reactions. Nevertheless, their incidence and participation in the physiopathology of autoimmune diseases have not been thoroughly studied...
August 25, 2016: Immunologic Research
Suvro Sankha Datta, Mahua Reddy, Sabita Basu, Shekhar Krishnan
A 12-year-old male child was presented in the emergency with features of anemia and mild icterus on day+67 of HSCT. The child was suffering from Fanconi anemia and undergone HSCT from ABO-matched, fully HLA matched sibling donor. The diagnosis of mixed type AIHA due to cytomegalovirus reactivation was made in the immunohematology laboratory and blood group discrepancy was the first sign of AIHA in this patient. Though the cold agglutinin titer was not significant but the clinical symptoms and laboratory evidences were suggestive of significant hemolysis due to underlying IgG autoantibody...
June 2016: Indian Journal of Hematology & Blood Transfusion
Jose Manuel Vagace, Rocío Cardesa, Antonio Corbacho, Teresa Vázquez, Maria Dolores de la Maya, Fernando Ataulfo Gonzalez, José Bartolomé Nieto, Emilia Urrutia, María Jesus Gómez, Teresa Pascual, Maria Reyes Aguinaco, Guillermo Gervasini
Hyperhemolysis syndrome (HHS) is characterized by severe intravascular hemolysis with a decrease in the reticulocyte count, which is triggered and aggravated by transfusion and cannot be explained by standard immunohematological studies. A nationwide study was conducted in order to retrospectively identify thalassemia patients with HHS in Spain in order to assess pre-disposing mechanisms for this syndrome. For this, the expression of adhesion (CD49, CD36) and complement-related molecules (C3a, CD59) and the levels of reticulocyte apoptosis and macrophage activation were measured in 4 thalassemia patients with HHS, 14 patients without HHS, and 10 healthy subjects...
September 2016: Annals of Hematology
Karen M K De Vooght, Marlies Oostendorp, Wouter W van Solinge
PURPOSE OF REVIEW: Immunotherapeutic strategies are emerging as novel therapeutic approaches in multiple myeloma, with several mAbs being in advanced stages of clinical development. Of these, CD38 targeting antibodies appear very promising. In trials with anti-CD38 mAb daratumumab, all patients demonstrated panreactivity in red blood cell (RBC) panel testing, complicating the selection of compatible RBCs for transfusion. This review provides an overview of the interferences and solutions to safely transfuse these patients...
November 2016: Current Opinion in Hematology
T Bakchoul, R Jouni, T E Warkentin
Protamine is widely used in medicine as a rapidly-acting antidote to heparin, particularly for reversing heparin anticoagulation after cardiac surgery. Protamine is also used as a stabilizing additive to certain preparations of insulin. Recent reports demonstrate that protamine and heparin form multimolecular complexes that result in high rates of immunization in post-cardiac surgery patients, particularly of immunoglobulin G (IgG) class antibodies; a subset of these anti-protamine/heparin IgG antibodies activates platelets through their FcγIIA (IgG) receptors...
September 2016: Journal of Thrombosis and Haemostasis: JTH
Anoosha Habibi, Armand Mekontso-Dessap, Constance Guillaud, Marc Michel, Keyvan Razazi, Mehdi Khellaf, Btissam Chami, Dora Bachir, Claire Rieux, Giovanna Melica, Bertrand Godeau, Frédéric Galacteros, Pablo Bartolucci, France Pirenne
Delayed hemolytic transfusion reaction (DHTR) is one of the most feared complications of sickle-cell disease (SCD). We retrospectively analyzed the clinical and biological features, treatments and outcomes of 99 DHTRs occurring in 69 referral center patients over 12 years. The first clinical signs appeared a median of 9.4 [IQR, 3-22] days after the triggering transfusion (TT). The most frequent DHTR-related clinical manifestation was dark urine/hemoglobinuria (94%). Most patients (89%) had a painful vaso-occlusive crisis and 50% developed a secondary acute chest syndrome (ACS)...
October 2016: American Journal of Hematology
Jeanne E Hendrickson, Christopher A Tormey
Red blood cell (RBC) transfusion is a cornerstone of the management of patients with hematology/oncology disorders. However, a potentially deleterious consequence of transfusion is the development of alloantibodies against blood group antigens present on RBCs. Such alloantibodies can be an obstacle in providing compatible units for transfusion. Providers in this arena must fully understand the testing performed by blood banks, as well as the consequences of detected antibodies. This article reviews immunohematologic tests, describes how autoimmune hemolytic anemia is classified by autoantibodies; outlines RBC alloimmunization rates, and presents strategies to prevent/mitigate the impact of RBC alloimmunization...
June 2016: Hematology/oncology Clinics of North America
Ajit C Gorakshakar, Kanjaksha Ghosh
Lectins are carbohydrate binding proteins present in seeds of many plants, especially corals and beans, in fungi and bacteria, and in animals. Apart from their hemagglutinating property, a wide range of functions have been attributed to them. Their importance in the area of immunohematology is immense. They are used to detect specific red cell antigens, to activate different types of lymphocytes, in order to resolve problems related to polyagglutination and so on. The introduction of advanced biotechnological tools generates new opportunities to exploit the properties of lectins, which were not used earlier...
January 2016: Asian Journal of Transfusion Science
Rainer Moog
BACKGROUND: In times of patient blood management, studies focus on appropriate transfusion strategies to improve patient's outcome. Little is known about the quality assurance in preparation of blood transfusion and thereafter. In the present study, the indication, informed consent, mandatory immunohematology tests and documentation of blood transfusions were evaluated using assessed self-inspection forms approved by the hospital transfusion committee. MATERIALS AND METHODS: 47 medical records of transfused patients were retrospectively reviewed by a clinical transfusion audit team in six hospital facilities from 2012 to 2015...
June 2016: Transfusion and Apheresis Science
Ashif Jethava, Esperanza Olivares, Sherry Shariatmadar
Anti-Di(a) is a clinically significant red cell antibody known to cause hemolytic disease of the newborn. Here, we report on a case of mild hemolytic disease of the newborn caused by Di(a) antibody. The mother had three prior pregnancies with no history of blood transfusion. She delivered a preterm 35-week-old female newborn by cesarean section. The neonate developed anemia and mild icterus on postnatal day five with hemoglobin of 9500 mg/dL and total bilirubin of 10 mg/dL. The direct antiglobulin test on the neonate's red blood cells was positive...
2015: Case Reports in Pediatrics
Christopher A Tormey, Manjula Santhanakrishnan, Nicole H Smith, Jingchun Liu, Susanne Marschner, Raymond P Goodrich, Jeanne E Hendrickson
BACKGROUND: Ultraviolet (UV) illumination/pathogen reduction effectively inactivates white blood cells (WBCs) in whole blood. Given that cotransfused WBCs may impact recipient immune responses, we hypothesized that pathogen reduction of whole blood may alter responses to RBC antigens. STUDY DESIGN AND METHODS: Transgenic mice expressing a model (HOD) antigen, authentic human (hGPA or KEL) antigens, or natural fluorescence (uGFP) on their RBCs were utilized as blood donors...
April 2016: Transfusion
Stephanie A Boccoz, Gaelle C Le Goff, Celine A Mandon, Benjamin P Corgier, Loïc J Blum, Christophe A Marquette
Thirty-five blood group systems, containing >300 antigens, are listed by the International Society of Blood Transfusion. Most of these antigens result from a single nucleotide polymorphism. Blood group typing is conventionally performed by serology. However, this technique has some limitations and cannot respond to the growing demand of blood products typed for a large number of antigens. The knowledge of the molecular basis of these red blood cell systems allowed the implementation of molecular biology methods in immunohematology laboratories...
January 2016: Journal of Molecular Diagnostics: JMD
E Strobel, P Gleich
PURPOSE OF THE STUDY: Not only in blood donation services, but also in the immunohaematological laboratory of a hospital including the depository for blood products a hygiene plan must be drawn up and its realization has to be documented. MATERIAL AND METHODS: From 2011 to 2014, some equipment in the depository and in the immunohematological laboratory was microbiologically monitored once a year. The examinations were done by direct contact cultures taken from several places of each device...
October 2015: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
Raj Nath Makroo, Anita Kaul, Aakanksha Bhatia, Soma Agrawal, Chanchal Singh, Prashant Karna
Anti-G has been reported as a possible cause of hemolytic disease of the fetus and newborn (HDFN), either independently or in association with anti-D, anti-C or both. The antibody mimics the pattern of anti-C and anti-D reactivity in the identification panel and is often present along with either or both of these antibodies. The differentiation of anti-D, -C and-G in routine pretransfusion workup is particularly essential in antenatal cases. We report two antenatal cases where anti-G was identified on advanced immunohematological workup, in addition to other alloantibodies...
July 2015: Asian Journal of Transfusion Science
Sabita Basu, Supriya Dhar, Deepak Mishra, Mammen Chandy
BACKGROUND: The ABO blood group system is of prime significance in red cell transfusion and organ transplantation. However, ABO compatibility is not critical in allogenic hemopoietic stem cell transplantation (HSCT) and approximately 40-50% of hemopoietic stem cell transplants are ABO incompatible. This incompatibility may be major, minor or bi-directional. Though there are descriptions of transfusion practice and protocols in ABO incompatible HSCT, there are considerable variations and transfusion support in these patients can be very challenging...
July 2015: Asian Journal of Transfusion Science
Tangui Le Guen, Fabien Touzot, Isabelle André-Schmutz, Chantal Lagresle-Peyrou, Benoit France, Laetitia Kermasson, Nathalie Lambert, Capucine Picard, Patrick Nitschke, Wassila Carpentier, Christine Bole-Feysot, Annick Lim, Marina Cavazzana, Isabelle Callebaut, Jean Soulier, Nada Jabado, Alain Fischer, Jean-Pierre de Villartay, Patrick Revy
BACKGROUND: Myb-Like, SWIRM, and MPN domains 1 (MYSM1) is a metalloprotease that deubiquitinates the K119-monoubiquitinated form of histone 2A (H2A), a chromatin marker associated with gene transcription silencing. Likewise, it has been reported that murine Mysm1 participates in transcription derepression of genes, among which are transcription factors involved in hematopoietic stem cell homeostasis, hematopoiesis, and lymphocyte differentiation. However, whether MYSM1 has a similar function in human subjects remains unclear...
December 2015: Journal of Allergy and Clinical Immunology
Patrick Celis, Nicolas Ferry, Marit Hystad, Martina Schüßler-Lenz, Pieter A Doevendans, Egbert Flory, Claire Beuneu, Ilona Reischl, Paula Salmikangas
On September 11, 2014, a workshop entitled 'Advanced Therapy Medicinal Products: How to Bring Cell-Based Medicinal Product Successfully to the Market' was held at the 47th annual meeting of the German Society for Transfusion Medicine and Immunohematology (DGTI), co-organised by the European Medicines Agency (EMA) and the DGTI in collaboration with the German Stem Cell Network (GSCN). The workshop brought together over 160 participants from academia, hospitals, small- or medium-sized enterprise developers and regulators...
May 2015: Transfusion Medicine and Hemotherapy
Gyorgy Abel
In the past half-century, routine central laboratory testing has become increasingly automated and efficient. The majority of clinical chemistry, immunochemistry and hematology testing are performed using high throughput instrumentation, with sophisticated automation. Microbiology, immunohematology and molecular diagnostic testing are also becoming increasingly automated. Recent challenges in healthcare demand new diagnostic solutions worldwide. Point-of-care testing (POCT) offers considerable advantages over central laboratory testing, such as fast and simple specimen handling, and simpler sample requirement (no additives and mostly blood from finger stick; and urine)...
2015: Expert Review of Molecular Diagnostics
Connie M Westhoff, Paul M Ness
No abstract text is available yet for this article.
June 2015: Transfusion
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