keyword
MENU ▼
Read by QxMD icon Read
search

immunohematology

keyword
https://www.readbyqxmd.com/read/28425753/applications-of-selected-cells-in-immunohematology-in-a-developing-country-case-studies
#1
Ravi C Dara, Aseem K Tiwari, Dinesh Arora, Subhasis Mitra, Geet Aggarwal, Devi P Acharya, Gunjan Bhardwaj
When an antibody is detected, its specificity should be determined and its likely clinical significance should be assessed. When one antibody has been identified, it becomes necessary to confirm the presence of additional significant antibodies to ensure that compatible blood is provided to the patient. To perform this confirmation, specific reagent red blood cells (RBCs) are selected; these are called selected cells. Though the most common use of selected cells is for antibody confirmation, they can also be used for several other immunohematologic applications...
January 2017: Immunohematology
https://www.readbyqxmd.com/read/28425751/development-of-red-blood-cell-autoantibodies-following-treatment-with-checkpoint-inhibitors-a-new-class-of-anti-neoplastic-immunotherapeutic-agents-associated-with-immune-dysregulation
#2
Laura L Cooling, John Sherbeck, Jonathon C Mowers, Sheri L Hugan
Ipilimumab, nivolumab, and pembrolizumab represent a new class of immunotherapeutic drugs for treating patients with advanced cancer. Known as checkpoint inhibitors, these drugs act to upregulate the cellular and humoral immune response to tumor antigens by inhibiting T-cell autoregulation. As a consequence, they can be associated with immune-related adverse events (irAEs) due to loss of self-tolerance, including rare cases of immune-related cytopenias. We performed a retrospective clinical chart review, including serologic, hematology, and chemistry laboratory results, of two patients who developed red blood cell (RBC) autoantibodies during treatment with a checkpoint inhibitor...
January 2017: Immunohematology
https://www.readbyqxmd.com/read/28425748/a-field-analysis-trial-comparing-the-turnaround-times-of-routine-and-stat-red-blood-cell-immunohematology-testing
#3
Katie Sackett, Andrea Kjell, Abigail M Schneider, Claudia S Cohn
The turnaround time (TAT) for pre-transfusion testing is important for prompt clinical decision-making. TAT includes the time between the arrival of the sample and the initiation of testing, plus the processing time (PT) required to generate and report a result. The TAT in larger blood banks is mostly dependent upon the capability of the analyzer used. In smaller blood banks, where manual work is often performed, the TAT is dependent on availability and experience of staff, testing resources, and workload. Our site performed a comparative analysis of the ORTHO VISION® (Ortho Clinical Diagnostics, Raritan, NJ) and the Echo® (Immucor, Norcross, GA) blood bank analyzers, using the TAT and PT of standard blood bank tests as the outcome metrics...
January 2017: Immunohematology
https://www.readbyqxmd.com/read/28424562/immunological-and-hematological-reference-intervals-among-hiv-seronegative-pregnant-women-in-northwest-ethiopia
#4
Meaza Genetu, Debasu Damtie, Meseret Workineh, Biniam Mathewos Tebeje, Bamlaku Enawgaw, Tekalign Deressa
BACKGROUND: Pregnancy is a state characterized by profound physiological hematological changes. However, hematological reference intervals being used in clinical practice in Ethiopia are derived from nonlocal general populations, despite the significant variations reported previously. The aim of this study was to determine the immunological and hematological reference intervals in healthy pregnancy among HIV-seronegative pregnant women in northwest Ethiopia. MATERIALS AND METHODS: A total of 200 healthy, HIV-seronegative pregnant women were enrolled from February 2015 to June 2015 in a cross-sectional study setting at Gondar University Hospital...
2017: International Journal of Women's Health
https://www.readbyqxmd.com/read/28416603/high-throughput-immunogenetics-for-clinical-and-research-applications-in-immunohematology-potential-and-challenges
#5
Anton W Langerak, Monika Brüggemann, Frédéric Davi, Nikos Darzentas, Jacques J M van Dongen, David Gonzalez, Gianni Cazzaniga, Véronique Giudicelli, Marie-Paule Lefranc, Mathieu Giraud, Elizabeth A Macintyre, Michael Hummel, Christiane Pott, Patricia J T A Groenen, Kostas Stamatopoulos
Analysis and interpretation of Ig and TCR gene rearrangements in the conventional, low-throughput way have their limitations in terms of resolution, coverage, and biases. With the advent of high-throughput, next-generation sequencing (NGS) technologies, a deeper analysis of Ig and/or TCR (IG/TR) gene rearrangements is now within reach, which impacts on all main applications of IG/TR immunogenetic analysis. To bridge the generation gap from low- to high-throughput analysis, the EuroClonality-NGS Consortium has been formed, with the main objectives to develop, standardize, and validate the entire workflow of IG/TR NGS assays for 1) clonality assessment, 2) minimal residual disease detection, and 3) repertoire analysis...
April 17, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28152331/comparison-of-immunohematological-profile-between-endurance-and-power-oriented-elite-athletes
#6
COMPARATIVE STUDY
José P Morgado, Catarina N Matias, Cristina P Monteiro, Francisco Alves, Joana F Reis, Diana A Santos, Analiza M Silva, Fátima Martins, Maria T Seixas, Petronila Rocha-Pereira, Luís B Sardinha, Maria J Laires
There is general perception that elite athletes are highly susceptible to changes in immunohematological profile. The objective of this study was to compare immunohematological parameters of elite athletes of different aerobic and muscular strength sports and analyze changes over 2 months. Sixteen judoists and 14 swimmers were evaluated 2 months before (M1) and immediately prior to competition (M2). Hemogram and lymphocytes subpopulations were assessed with automatic counter and flow cytometry, respectively...
March 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28150308/practical-approaches-and-costs-for-provisioning-safe-transfusions-during-anti-cd38-therapy
#7
Waseem Q Anani, Marisela G Marchan, Kathleen M Bensing, Michael Schanen, Cindy Piefer, Jerome L Gottschall, Gregory A Denomme
BACKGROUND: Anti-CD38 therapy causes interference with both the direct and the indirect antiglobulin tests. We describe the experience from an Immunohematology Reference Laboratory and model cost options for providing safe transfusions. STUDY DESIGN AND METHODS: Phenotyping, genotyping, and antibody identification orders were retrospectively reviewed in the setting of anti-CD38 therapy. The data were used to model the added cost of transfusion support. Four approaches were evaluated: 1) thiol-treated reagent red blood cells (RRCs) in antibody investigations with K- red blood cell (RBC) transfusions, 2) patient phenotyping or 3) genotyping with antigen-matched RBC transfusions, and 4) a combination of interval thiol-treated RRC antibody investigations with genotype antigen-matched RBC transfusions...
February 1, 2017: Transfusion
https://www.readbyqxmd.com/read/28138088/multi-center-evaluation-of-the-automated-immunohematology-instrument-the-ortho-vision-analyzer
#8
Agnes Aysola, Leslie Wheeler, Richard Brown, Rebecca Denham, Connie Colavecchia, Katerina Pavenski, Elizabeth Krok, Chelsea Hayes, Ellen Klapper
BACKGROUND: ORTHO VISION Analyzer (Vision), is an immunohematology instrument using ID-MT gel card technology with digital image processing. It has a continuous, random sample access with STAT priority processing. The efficiency and ease of operation of Vision was evaluated at 5 medical centers. METHODS: De-identified patient samples were tested on the ORTHO ProVue Analyzer (ProVue) and repeated on the Vision mimicking the daily workload pattern. Turnaround times (TAT) were collected and compared...
February 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/28097699/maternal-red-blood-cell-alloantibodies-identified-in-blood-samples-obtained-from-iranian-pregnant-women-the-first-population-study-in-iran
#9
Ehsan Shahverdi, Mostafa Moghaddam, Fateme Gorzin
BACKGROUND: The objective was to determine the frequency of occurrence of alloantibodies among pregnant women in Iran. STUDY DESIGN AND METHODS: This was a prospective cross-sectional study, which was carried out in the immunohematology reference laboratory of the Iranian Blood Transfusion Organization in Tehran, Iran, in 2008 to 2015. Screening and identification of red blood cell (RBC) alloantibodies was done on the sera of 7340 pregnant females using the standard tube method and gel column agglutination technique...
January 2017: Transfusion
https://www.readbyqxmd.com/read/28017498/red-cell-immunohematology-research-conducted-in-china
#10
REVIEW
Ziyan Zhu, Luyi Ye, Qin Li, Hongwei Gao, Yinxia Tan, Wei Cai
ABO subtypes and RhD variants are the most studied blood groups in China. Some of the polymorphisms in these two blood groups have direct clinical relevance. Molecular diagnosis of blood group polymorphisms is underway in China. In addition, research groups have developed methods such as screening for blood group mimetic peptides using phage display technology. New reagents, akin to antibodies directed against RhD and ABO, are being investigated using aptamer-based techniques. Progress is also being made in the development of synthetic exoglycosidases for conversion of group A and/or B antigens to group O...
November 24, 2016: Transfusion Medicine Reviews
https://www.readbyqxmd.com/read/27917465/warm-reactive-immunoglobulin-g-autoantibodies-and-laboratory-testing-best-practices-review-of-the-literature-and-survey-of-current-practice
#11
REVIEW
Alyssa Ziman, Claudia Cohn, Patricia M Carey, Nancy M Dunbar, Mark K Fung, Andreas Greinacher, Simon Stanworth, Nancy M Heddle, Meghan Delaney
BACKGROUND: Warm-reactive autoantibodies (WAAs) are the most common cause of autoimmune hemolytic anemia (AIHA) and can also be present without clinically significant hemolysis. WAAs complicate immunohematological testing, yet there is no commonly accepted approach to laboratory evaluation and red blood cell (RBC) selection. STUDY DESIGN AND METHODS: We searched PubMed/Cochrane Central for articles that described testing methodology and blood selection for patients with WAAs...
December 4, 2016: Transfusion
https://www.readbyqxmd.com/read/27708169/the-need-for-education-in-molecular-immunohematology-a-survey-of-specialists-in-blood-banking
#12
Stephanie N Kinney, Jayanna Slayten, Dan A Waxman
BACKGROUND: Within transfusion medicine, the education of molecular technologies lacks standardization. OBJECTIVE: To address this problem, we surveyed specialist in blood bank technology (SBBT) programs, immunohematology reference laboratories, and SBBT graduates to define its current state. METHODS: An anonymous online survey (SurveyMonkey) was emailed to the 15 American Association of Blood Banks (AABB) SBBT programs, 59 AABB IRLs, and 82 SBBT graduates...
November 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27561786/irregular-antibodies-in-no-hemolytic-autoimmune-diseases-are-able-to-induce-erythrophagocytosis
#13
Paola Ester López-Díaz, María Del Rocío Ruiz-Olivera, Luis Alberto Hernández-Osorio, Jaime Vargas-Arzola, Xareni Valle-Jiménez, Sergio Roberto Aguilar-Ruiz, Honorio Torres-Aguilar
Irregular antibodies are produced by alloimmunization because of pregnancies or blood transfusions. They are called "irregular" due to target erythrocyte antigens from "rare blood systems," those different from the ABO system. Irregular antibodies have been widely investigated in immunohematology since their presence in blood donors may lead to difficulties in blood typing and in blood cross-matching, or to induce hemolytic transfusion reactions. Nevertheless, their incidence and participation in the physiopathology of autoimmune diseases have not been thoroughly studied...
August 25, 2016: Immunologic Research
https://www.readbyqxmd.com/read/27408394/blood-group-discrepancy-first-sign-of-autoimmune-hemolytic-anemia-after-hematopoietic-stem-cell-transplantation-in-a-child
#14
Suvro Sankha Datta, Mahua Reddy, Sabita Basu, Shekhar Krishnan
A 12-year-old male child was presented in the emergency with features of anemia and mild icterus on day+67 of HSCT. The child was suffering from Fanconi anemia and undergone HSCT from ABO-matched, fully HLA matched sibling donor. The diagnosis of mixed type AIHA due to cytomegalovirus reactivation was made in the immunohematology laboratory and blood group discrepancy was the first sign of AIHA in this patient. Though the cold agglutinin titer was not significant but the clinical symptoms and laboratory evidences were suggestive of significant hemolysis due to underlying IgG autoantibody...
June 2016: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/27392662/etiopathological-mechanisms-and-clinical-characteristics-of-hyperhemolysis-syndrome-in-spanish-patients-with-thalassemia
#15
Jose Manuel Vagace, Rocío Cardesa, Antonio Corbacho, Teresa Vázquez, Maria Dolores de la Maya, Fernando Ataulfo Gonzalez, José Bartolomé Nieto, Emilia Urrutia, María Jesus Gómez, Teresa Pascual, Maria Reyes Aguinaco, Guillermo Gervasini
Hyperhemolysis syndrome (HHS) is characterized by severe intravascular hemolysis with a decrease in the reticulocyte count, which is triggered and aggravated by transfusion and cannot be explained by standard immunohematological studies. A nationwide study was conducted in order to retrospectively identify thalassemia patients with HHS in Spain in order to assess pre-disposing mechanisms for this syndrome. For this, the expression of adhesion (CD49, CD36) and complement-related molecules (C3a, CD59) and the levels of reticulocyte apoptosis and macrophage activation were measured in 4 thalassemia patients with HHS, 14 patients without HHS, and 10 healthy subjects...
September 2016: Annals of Hematology
https://www.readbyqxmd.com/read/27389485/new-mab-therapies-in-multiple-myeloma-interference-with-blood-transfusion-compatibility-testing
#16
Karen M K De Vooght, Marlies Oostendorp, Wouter W van Solinge
PURPOSE OF REVIEW: Immunotherapeutic strategies are emerging as novel therapeutic approaches in multiple myeloma, with several mAbs being in advanced stages of clinical development. Of these, CD38 targeting antibodies appear very promising. In trials with anti-CD38 mAb daratumumab, all patients demonstrated panreactivity in red blood cell (RBC) panel testing, complicating the selection of compatible RBCs for transfusion. This review provides an overview of the interferences and solutions to safely transfuse these patients...
November 2016: Current Opinion in Hematology
https://www.readbyqxmd.com/read/27378603/protamine-heparin-induced-thrombocytopenia-a-review-of-the-serological-and-clinical-features-associated-with-anti-protamine-heparin-antibodies
#17
REVIEW
T Bakchoul, R Jouni, T E Warkentin
Protamine is widely used in medicine as a rapidly-acting antidote to heparin, particularly for reversing heparin anticoagulation after cardiac surgery. Protamine is also used as a stabilizing additive to certain preparations of insulin. Recent reports demonstrate that protamine and heparin form multimolecular complexes that result in high rates of immunization in post-cardiac surgery patients, particularly of immunoglobulin G (IgG) class antibodies; a subset of these anti-protamine/heparin IgG antibodies activates platelets through their FcγIIA (IgG) receptors...
September 2016: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/27348613/delayed-hemolytic-transfusion-reaction-in-adult-sickle-cell-disease-presentations-outcomes-and-treatments-of-99-referral-center-episodes
#18
Anoosha Habibi, Armand Mekontso-Dessap, Constance Guillaud, Marc Michel, Keyvan Razazi, Mehdi Khellaf, Btissam Chami, Dora Bachir, Claire Rieux, Giovanna Melica, Bertrand Godeau, Frédéric Galacteros, Pablo Bartolucci, France Pirenne
Delayed hemolytic transfusion reaction (DHTR) is one of the most feared complications of sickle-cell disease (SCD). We retrospectively analyzed the clinical and biological features, treatments and outcomes of 99 DHTRs occurring in 69 referral center patients over 12 years. The first clinical signs appeared a median of 9.4 [IQR, 3-22] days after the triggering transfusion (TT). The most frequent DHTR-related clinical manifestation was dark urine/hemoglobinuria (94%). Most patients (89%) had a painful vaso-occlusive crisis and 50% developed a secondary acute chest syndrome (ACS)...
October 2016: American Journal of Hematology
https://www.readbyqxmd.com/read/27113001/red-blood-cell-antibodies-in-hematology-oncology-patients-interpretation-of-immunohematologic-tests-and-clinical-significance-of-detected-antibodies
#19
REVIEW
Jeanne E Hendrickson, Christopher A Tormey
Red blood cell (RBC) transfusion is a cornerstone of the management of patients with hematology/oncology disorders. However, a potentially deleterious consequence of transfusion is the development of alloantibodies against blood group antigens present on RBCs. Such alloantibodies can be an obstacle in providing compatible units for transfusion. Providers in this arena must fully understand the testing performed by blood banks, as well as the consequences of detected antibodies. This article reviews immunohematologic tests, describes how autoimmune hemolytic anemia is classified by autoantibodies; outlines RBC alloimmunization rates, and presents strategies to prevent/mitigate the impact of RBC alloimmunization...
June 2016: Hematology/oncology Clinics of North America
https://www.readbyqxmd.com/read/27011665/use-of-lectins-in-immunohematology
#20
REVIEW
Ajit C Gorakshakar, Kanjaksha Ghosh
Lectins are carbohydrate binding proteins present in seeds of many plants, especially corals and beans, in fungi and bacteria, and in animals. Apart from their hemagglutinating property, a wide range of functions have been attributed to them. Their importance in the area of immunohematology is immense. They are used to detect specific red cell antigens, to activate different types of lymphocytes, in order to resolve problems related to polyagglutination and so on. The introduction of advanced biotechnological tools generates new opportunities to exploit the properties of lectins, which were not used earlier...
January 2016: Asian Journal of Transfusion Science
keyword
keyword
33889
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"