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https://www.readbyqxmd.com/read/28549208/identification-of-reference-genes-for-rt-qpcr-analysis-in-peach-genotypes-with-contrasting-chilling-requirements
#1
N Marini, C B Bevilacqua, M V Büttow, M C B Raseira, S Bonow
Selecting and validating reference genes are the first steps in studying gene expression by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). The present study aimed to evaluate the stability of five reference genes for the purpose of normalization when studying gene expression in various cultivars of Prunus persica with different chilling requirements. Flower bud tissues of nine peach genotypes from Embrapa's peach breeding program with different chilling requirements were used, and five candidate reference genes based on the RT-qPCR that were useful for studying the relative quantitative gene expression and stability were evaluated using geNorm, NormFinder, and bestKeeper software packages...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28547383/complete-nucleotide-sequence-of-a-highly-divergent-cherry-associated-luteovirus-chalv-isolate-from-peach-in-south-korea
#2
Davaajargal Igori, Seungmo Lim, Dasom Baek, In Sook Cho, Jae Sun Moon
We determined the complete genome sequence of a highly divergent South Korean (SK) isolate of a cherry-associated luteovirus (ChALV) from peach. The ChALV-SK genome consists of 5,815 nucleotides, and contains five open reading frames (ORFs). A comparative analysis of the full genome showed only 73.1% nucleotide sequence identity with a recently described ChALV from the Czech Republic (CZ). Amino acid similarities of the individual ORFs between ChALV-SK and other luteoviruses range from 17.3 to 92%, which places the new isolate close to the species demarcation value for luteoviruses...
May 25, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28542315/attitudes-of-older-people-with-mild-dementia-and-mild-cognitive-impairment-and-their-relatives-about-falls-risk-and-prevention-a-qualitative-study
#3
Tamsin Peach, Kristian Pollock, Veronika van der Wardt, Roshan das Nair, Pip Logan, Rowan H Harwood
OBJECTIVE: To explore the perceptions of older people with mild dementia and mild cognitive impairment, and their family carers, about falling, falls risk and the acceptability of falls prevention interventions. DESIGN: Qualitative study involving thematic analysis of semi-structured interviews with patient and relative dyads. PARTICIPANTS AND SETTING: 20 patient/ relative dyads recruited from Memory Assessment Services and Falls Prevention Services in the United Kingdom...
2017: PloS One
https://www.readbyqxmd.com/read/28541388/the-genome-sequence-of-sweet-cherry-prunus-avium-for-use-in-genomics-assisted-breeding
#4
Kenta Shirasawa, Kanji Isuzugawa, Mitsunobu Ikenaga, Yutaro Saito, Toshiya Yamamoto, Hideki Hirakawa, Sachiko Isobe
We determined the genome sequence of sweet cherry (Prunus avium) using next-generation sequencing technology. The total length of the assembled sequences was 272.4 Mb, consisting of 10,148 scaffold sequences with an N50 length of 219.6 kb. The sequences covered 77.8% of the 352.9 Mb sweet cherry genome, as estimated by k-mer analysis, and included >96.0% of the core eukaryotic genes. We predicted 43,349 complete and partial protein-encoding genes. A high-density consensus map with 2,382 loci was constructed using double-digest restriction site-associated DNA sequencing...
May 25, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28534760/minibrain-storm-cerebral-organoids-aren-t-real-brains-but-they-provide-a-powerful-platform-for-modeling-brain-diseases-like-zika-infection-alzheimer-s-and-even-autism
#5
Shannon Fischer
Floating in a Petri dish, they look like tiny tapioca pearls in peach broth, a couple dozen in number and none much larger than the tip of a ballpoint pen. But under a microscope, dense, lumpy bodies come into focus, outlined by wispy coronas.
May 2017: IEEE Pulse
https://www.readbyqxmd.com/read/28520891/sublethal-effects-of-thiamethoxam-on-the-demographic-parameters-of-myzus-persicae-hemiptera-aphididae
#6
Pan Wang, Li-Lin Zhou, Fan Yang, Mang Li, Xiao-Ming Liu, Yong Wang, Chao-Liang Lei, Sheng-Yun Si
The green peach aphid, Myzus persicae Sulzer (Hemiptera: Aphididae), is an important sap-sucking pest of many crops, including Chinese cabbage, Brassinca oleracea L. The neonicotinoid insecticide thiamethoxam has been used as an effective insecticide to control M. persicae in cabbage fields. In this study, we assessed the effects of sublethal concentrations of thiamethoxam on demographic parameters of M. persicae. In leaf-dip bioassays, thiamethoxam showed a relatively high toxicity against M. persicae with an LC50 of 6...
May 17, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28510728/spatial-and-temporal-dynamics-of-aphids-hemiptera-aphididae-in-the-columbia-basin-and-northeastern-oregon
#7
Mathew L Klein, Silvia I Rondon, Darrin L Walenta, Qamar Zeb, Alexzandra F Murphy
Aphid species, such as the potato aphid, Macrosiphum euphorbiae Thomas, and the green peach aphid, Myzus persicae Sulzer, are routinely considered the most important pests of potatoes. Potato aphid, green peach aphid, and more recently, other aphids such as the bird cherry-oat aphid Rhopalosiphum padi L. have been identified as vectors of multiple plant pathogenic viruses in potatoes. Since 2006, an area-wide trapping network consisting of ∼60 sites was developed through collaboration between researchers, extension faculty, and stakeholders, to monitor aphid populations in the Columbia Basin of Oregon (Umatilla and Morrow counties) and in northeastern Oregon (Union and Baker counties)...
May 16, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28509799/nitrous-oxide-from-a-peach-of-a-specialist-j-warren-worcester-m-d
#8
(no author information available yet)
No abstract text is available yet for this article.
June 2017: Anesthesiology
https://www.readbyqxmd.com/read/28505165/determination-of-fruit-maturity-and-its-prediction-model-based-on-the-pericarp-index-of-absorbance-difference-iad-for-peaches
#9
Binbin Zhang, Bin Peng, Chunhua Zhang, Zhizhong Song, Ruijuan Ma
Harvest maturity is closely related to peach fruit quality and has a very important effect on the fresh fruit market. Unfortunately, at present, it is difficult to determine the maturity level of peach fruits by artificial methods. The objectives of this study were to develop quadratic polynomial regression models using near-infrared spectroscopy that could determine the peel color difference, fruit firmness, soluble solids content (SSC), soluble sugar, organic acid components, and their relationships with the absorbance of chlorophyll (index of absorbance difference, IAD) in late maturing 'Xiahui 8' peach and 'Xiaguang' nectarine fruits...
2017: PloS One
https://www.readbyqxmd.com/read/28496992/flavored-little-cigar-smoke-induces-cytotoxicity-and-apoptosis-in-airway-epithelia
#10
Arunava Ghosh, Rachel C Nethery, Amy H Herring, Robert Tarran
Addition of flavors reduces the harsh taste of tobacco, facilitating the initiation and maintenance of addiction among youths. Flavored cigarettes (except menthol) are now banned. However, the legislation on little cigars remains unclear and flavored little cigars are currently available for purchase. Since inhaled tobacco smoke directly exerts toxic effects on the lungs, we tested whether non-flavored and flavored little cigar smoke exposure had the potential for harm in cultured pulmonary epithelia. We cultured Calu-3 lung epithelia on both 96-well plates and at the air-liquid interface and exposed them to smoke from non-flavored Swisher Sweets and flavored (sweet cherry, grape, menthol, peach and strawberry) Swisher Sweets little cigars...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28494408/solubilisation-of-oils-in-aqueous-solutions-of-a-random-cationic-copolymer
#11
Jonathan C Pegg, Adam Czajka, Gavin Hazell, Christopher Hill, Jocelyn Peach, Sarah E Rogers, Julian Eastoe
HYPOTHESIS: Reports of random copolymers capable of solubilising hydrophobic oils are rare. This is primarily because random copolymers are unlikely to self-assemble into suitable aggregates (or micelles) in water. A random copolymer with a "blocky" (or lumpy) microstructure may have potential to solubilise hydrophobic oils in water. This type of polymer would have advantages over block copolymers which are more laborious and costly to synthesise. EXPERIMENTS: The solubilising capacity of a blocky random copolymer, namely poly(methyl methacrylate-co-2-dimethylaminoethyl methacrylate) (PMMA-co-PDMAEMA) is assessed by UV-visible spectroscopy and compared with common reference surfactants...
May 2, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28491939/data-on-water-consumption-in-streptozotocin-induced-diabetic-mice-by-a-novel-peach-gum-derived-polysaccharide
#12
Yuting Wang, Dingbo Lin, Xiaoli Wang, Wei Zhu, Junli Ye, Guohuai Li, Zhaocheng Ma, Xiuxin Deng
The data presented in this article are related to the article entitled "The impact of a novel peach gum-derived polysaccharide on postprandial blood glucose control in streptozotocin-induced diabetic mice" (Wang et al., 2017) [1]. Polydipsia was one of the most important symptoms of diabetic mellitus (DM) mice, which showed more water consumption than normal ones. The water consumption of DM mice in different groups administrated with metformin hydrochloride or a novel polysaccharide (coded as PGPSD) were exhibited in this article (Fig...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28490107/simultaneous-estimation-of-scavenging-capacities-of-peach-extract-for-multiple-reactive-oxygen-species-by-fluorescence-fingerprint-method
#13
Vipavee Trivittayasil, Hiromi Kameya, Toshihiko Shoji, Mizuki Tsuta, Mito Kokawa, Junichi Sugiyama
In this study, the potential of using fluorescence fingerprint, also known as fluorescence excitation-emission matrix, for estimating the scavenging capacity of peach extract on reactive oxygen species (ROS) was investigated. Samples from each of the five cultivars (Asama Hakuto, Hakuho, Kawanakajima Hakuto, Natsukko and Ougonto) were freeze-dried and crushed. The scavenging capacities of peach extracts for the target ROS (hydroxyl, superoxide, alkoxyl radicals and singlet oxygen) were measured by electron paramagnetic resonance spin trapping method...
October 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28486404/efficacy-of-chemicals-for-the-potential-management-of-the-queensland-fruit-fly-bactrocera-tryoni-froggatt-diptera-tephritidae
#14
Olivia L Reynolds, Terrence J Osborne, Idris Barchia
This study investigated alternative in-field chemical controls against Bactrocera tryoni (Froggatt). Bioassay 1 tested the mortality of adults exposed to fruit and filter paper dipped in insecticide, and the topical application of insecticide to adults/fruit. Bioassay 2 measured the mortality of adults permitted to oviposit on fruit dipped in insecticide and aged 0, 1, 3, or 5 days, plus the production of offspring. Bioassay 3 tested infested fruit sprayed with insecticide. The field bioassay trialed the mortality of adults exposed to one- and five-day insecticide residues on peaches, and subsequent offspring...
May 9, 2017: Insects
https://www.readbyqxmd.com/read/28485472/relevance-of-the-main-postharvest-handling-operations-on-the-development-of-brown-rot-disease-on-stone-fruits
#15
M Bernat, J Segarra, C Casals, N Teixidó, R Torres, J Usall
BACKGROUND: Brown rot caused by Monilinia spp. is one of the most important postharvest diseases of stone fruit. The aim of this study was evaluate the relevance of the main postharvest operations of fruit; hydrocooling, cold room, water dump, sorting and cooling tunnel in the development of M. laxa on peaches and nectarines artificially infected 48, 24 or 2 hours before postharvest operations. RESULTS: Commercial hydrocooling operation reduced incidence to 10 % in 'Pp 100' nectarine inoculated 2 and 24 hours before this operation however in 'Fantasia' nectarine incidence was not reduced, although lesion diameter was decreased in all studied varieties...
May 9, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28482318/differential-responses-of-peach-prunus-persica-seedlings-to-elevated-ozone-are-related-with-leaf-mass-per-area-antioxidant-enzymes-activity-rather-than-stomatal-conductance
#16
Lulu Dai, Pin Li, Bo Shang, Shuo Liu, Aizhen Yang, Younian Wang, Zhaozhong Feng
To evaluate the ozone (O3) sensitivity among peach tree (Prunus persica) cultivars widely planted in Beijing region and explore the possible eco-physiological response mechanisms, thirteen cultivars of peach seedlings were exposed to either charcoal-filtered air or elevated O3 (E-O3, non-filtered ambient air plus 60 ppb) for one growing season in open-top chambers. Leaf structure, stomatal structure, gas exchange and chlorophyll a fluorescence, photosynthetic pigments, antioxidant defense system and lipid peroxidation were measured in three replicated chambers...
May 5, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28475682/susceptibility-of-australian-myzus-persicae-hemiptera-aphididae-to-three-recently-registered-insecticides-spirotetramat-cyantraniliprole-and-sulfoxaflor
#17
Siobhan C de Little, Paul A Umina
The green peach aphid, Myzus persicae (Sulzer), is a significant agricultural pest that has developed resistance to a large number of insecticides globally. Within Australia, resistance has previously been confirmed for multiple chemical groups, including pyrethroids, carbamates, organophosphates, and neonicotinoids. In this study, we use leaf-dip and topical bioassays to investigate susceptibility and potential cross-resistance of 12 field-collected populations of Australian M. persicae to three recently registered insecticides: sulfoxaflor, spirotetramat, and cyantraniliprole...
May 5, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28466059/inducible-transgenic-tobacco-system-to-study-the-mechanisms-underlying-chlorosis-mediated-by-the-silencing-of-chloroplast-heat-shock-protein-90
#18
Sachin Ashok Bhor, Chika Tateda, Tomofumi Mochizuki, Ken-Taro Sekine, Takashi Yaeno, Naoto Yamaoka, Masamichi Nishiguchi, Kappei Kobayashi
Chlorosis is one of the most common symptoms of plant diseases, including those caused by viruses and viroids. Recently, a study has shown that Peach latent mosaic viroid (PLMVd) exploits host RNA silencing machinery to modulate the virus disease symptoms through the silencing of chloroplast-targeted heat shock protein 90 (Hsp90C). To understand the molecular mechanisms of chlorosis in this viroid disease, we established an experimental system suitable for studying the mechanism underlying the chlorosis induced by the RNA silencing of Hsp90C in transgenic tobacco...
March 2017: Virusdisease
https://www.readbyqxmd.com/read/28465637/colors-of-vegetables-and-fruits-and-the-risks-of-colorectal-cancer
#19
Jeeyoo Lee, Aesun Shin, Jae Hwan Oh, Jeongseon Kim
AIM: To investigate the relationship between the colors of vegetables and fruits and the risk of colorectal cancer in Korea. METHODS: A case-control study was conducted with 923 colorectal cancer patients and 1846 controls recruited from the National Cancer Center in Korea. We classified vegetables and fruits into four groups according to the color of their edible parts (e.g., green, orange/yellow, red/purple and white). Vegetable and fruit intake level was classified by sex-specific tertile of the control group...
April 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28448536/lateral-flow-immunoassay-for-on-site-detection-of-xanthomonas-arboricola-pv-pruni-in-symptomatic-field-samples
#20
Pablo López-Soriano, Patricia Noguera, María Teresa Gorris, Rosa Puchades, Ángel Maquieira, Ester Marco-Noales, María M López
Xanthomonas arboricola pv. pruni is a quarantine pathogen and the causal agent of the bacterial spot disease of stone fruits and almond, a major threat to Prunus species. Rapid and specific detection methods are essential to improve disease management, and therefore a prototype of a lateral flow immunoassay (LFIA) was designed for the detection of X. arboricola pv. pruni in symptomatic field samples. It was developed by producing polyclonal antibodies which were then combined with carbon nanoparticles and assembled on nitrocellulose strips...
2017: PloS One
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