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https://www.readbyqxmd.com/read/28337785/transcriptomic-and-metabolic-analyses-provide-new-insights-into-chilling-injury-in-peach-fruit
#1
Ke Wang, Xue-Ren Yin, Bo Zhang, Don Grierson, Chang-Jie Xu, Kun-Song Chen
Low temperature conditioning (LTC) alleviates peach fruit chilling injury but the underlying molecular basis is poorly understood. Here, changes in transcriptome, ethylene production, flesh softening, internal browning, and membrane lipids were compared in fruit maintained in constant 0 °C and LTC (pre-storage at 8 °C for 5 days before storage at 0 °C). LTC resulted in a higher rate of ethylene production and a more rapid flesh softening as a result of higher expression of ethylene biosynthetic genes and a series of cell wall hydrolases...
March 24, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28336950/dual-regulation-of-water-retention-and-cell-growth-by-a-stress-associated-protein-sap-gene-in-prunus
#2
Alba Lloret, Ana Conejero, Carmen Leida, César Petri, Francisco Gil-Muñoz, Lorenzo Burgos, María Luisa Badenes, Gabino Ríos
We have identified a gene (PpSAP1) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants. PpSAP1 was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release. PpSAP1 expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28330195/identification-and-characterization-of-endophytic-bacteria-isolated-from-in-vitro-cultures-of-peach-and-pear-rootstocks
#3
Fakhra Liaqat, Rengin Eltem
Endophytes are microorganisms which live symbiotically with almost all varieties of plant and in turn helping the plant in a number of ways. Instead of satisfactory surface sterilization approaches, repeatedly occurring bacterial growth on in vitro rootstock cultures of peach and pear was identified and isolated as endophytic bacteria in our present study. Five different isolates from peach rootstocks were molecularly identified by 16S rRNA gene sequencing as Brevundimonas diminuta, Leifsonia shinshuensis, Sphingomonas parapaucimobilis Brevundimonas vesicularis, Agrobacterium tumefaciens while two endophytic isolates of pear were identified as Pseudoxanthomonas mexicana, and Stenotrophomonas rhizophilia...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330149/myb-transcription-factor-isolated-from-raphanus-sativus-enhances-anthocyanin-accumulation-in-chrysanthemum-cultivars
#4
Eun Sun Kee, Aung Htay Naing, Sun Hyung Lim, Jeung Sul Han, Chang Kil Kim
A MYB transcription factor gene, RsMYB1, from radish was introduced into the chrysanthemum cultivars 'Peach ND', 'Peach Red', and 'Vivid Scarlet' under the control of the cauliflower mosaic virus 35S promoter. Presence of RsMYB1 in transgenic lines was confirmed using polymerase chain reaction (PCR). Results of reverse-transcription-PCR analysis revealed that the expression of RsMYB1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (F3H, DFR, and ANS) involved in anthocyanin production...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28326616/high-prevalence-of-sensitization-to-gibberellin-regulated-protein-peamaclein-in-fruit-allergies-with-negative-immunoglobulin-e-reactivity-to-bet-v-1-homologs-and-profilin-clinical-pattern-causative-fruits-and-cofactor-effect-of-gibberellin-regulated-protein
#5
Naoko Inomata, Mami Miyakawa, Michiko Aihara
Gibberellin-regulated protein (GRP) is a new allergen in peach allergy, with an amino acid sequence very well conserved through several botanical species. We investigated the allergenicity of GRP in fruit allergies other than peaches and identified the clinical characteristics of fruit allergy patients with GRP sensitization. One hundred consecutive Japanese patients with fruit allergies were enrolled in the present study. To identify the features of GRP sensitization, we selected patients with negative ImmunoCAP results for Bet v 1 homologs and profilin, which are marker allergens for pollen-food allergy syndrome (PFAS), or lipid transfer protein...
March 22, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/28320891/the-normal-ranges-of-cardiovascular-parameters-measured-using-the-ultrasonic-cardiac-output-monitor
#6
Giles N Cattermole, P Y Mia Leung, Grace Y L Ho, Peach W S Lau, Cangel P Y Chan, Stewart S W Chan, Brendan E Smith, Colin A Graham, Timothy H Rainer
The ultrasonic cardiac output monitor (USCOM) is a noninvasive transcutaneous continuous wave Doppler method for assessing hemodynamics. There are no published reference ranges for normal values in adults (aged 18-60 years) for this device. This study aimed to (1) measure cardiovascular indices using USCOM in healthy adults aged 18-60 years; (2) combine these data with those for healthy children (aged 0-12), adolescents (aged 12-18), and the elderly (aged over 60) from our previously published studies in order to present normal ranges for all ages, and (3) establish normal ranges of USCOM-derived variables according to both weight and age...
March 2017: Physiological Reports
https://www.readbyqxmd.com/read/28317175/occupational-respiratory-allergy-in-peach-crop-workers
#7
Remedios Pérez-Calderón, M Ángeles Gonzalo-Garijo, Francisco José Rodríguez-Velasco, Silvia Sánchez-Vega, Borja Bartolomé-Zavala
BACKGROUND: Occupational respiratory diseases in workers of peach tree crops have been reported punctually and have been associated with sensitization to proteins present in both pollen and leaf tree. We report the study of 37 workers with respiratory symptoms related to occupational exposure to peach trees. METHODS: Prick tests and specific IgE determinations were performed with extracts from leaves and branches of peach tree. Immunodetection in leaf extract was realized by SDS-PAGE-immunoblotting with patient sera and rabbit serum anti-Pru p 3...
March 20, 2017: Allergy
https://www.readbyqxmd.com/read/28314235/primary-and-secondary-pesticide-drift-profiles-from-a-peach-orchard
#8
Ohad Zivan, Yardena Bohbot-Raviv, Yael Dubowski
Atmospheric drift is considered a major loss path of pesticide from target areas, but there is still a large gap of knowledge regarding this complex phenomenon. Pesticide drift may occur during application (Primary drift) and after it (Secondary drift). The present study focuses on primary and secondary drift from ground applications in peach orchard (tree height of 3 m), under Mediterranean climate. Detailed and prolonged vertical drift profiles at close proximity to orchard are presented, together with detailed measurements of key meteorological parameters...
March 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/28299506/identification-of-the-ligand-of-pru-p-3-a-peach-ltp
#9
Nuria Cubells-Baeza, Cristina Gómez-Casado, Leticia Tordesillas, Carmen Ramírez-Castillejo, María Garrido-Arandia, Pablo González-Melendi, María Herrero, Luis F Pacios, Araceli Díaz-Perales
Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein...
March 15, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28290690/pomegranate-cultivars-identification-of-the-new-ige-binding-protein-pommaclein-and-analysis-of-anti-oxidant-variability
#10
Lisa Tuppo, Claudia Alessandri, Maria Silvia Pasquariello, Milena Petriccione, Ivana Giangrieco, Maurizio Tamburrini, Adriano Mari, Maria Antonietta Ciardiello
The consumption of pomegranate is increasing as it is considered a health-promoting food. Nevertheless, it can trigger allergic reactions, sometimes severe. The LTP Pun g 1 is the only pomegranate allergen so far reported. Based on preliminary clinical observations, the main aim of this study was the investigation of still unknown allergens contained in this fruit. Pommaclein, a homolog of peamaclein, the peach allergen Pru p 7, was isolated, identified by protein sequencing, and characterized as an IgE-binding protein by different test systems...
March 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28284188/the-peach-v2-0-release-high-resolution-linkage-mapping-and-deep-resequencing-improve-chromosome-scale-assembly-and-contiguity
#11
Ignazio Verde, Jerry Jenkins, Luca Dondini, Sabrina Micali, Giulia Pagliarani, Elisa Vendramin, Roberta Paris, Valeria Aramini, Laura Gazza, Laura Rossini, Daniele Bassi, Michela Troggio, Shengqiang Shu, Jane Grimwood, Stefano Tartarini, Maria Teresa Dettori, Jeremy Schmutz
BACKGROUND: The availability of the peach genome sequence has fostered relevant research in peach and related Prunus species enabling the identification of genes underlying important horticultural traits as well as the development of advanced tools for genetic and genomic analyses. The first release of the peach genome (Peach v1.0) represented a high-quality WGS (Whole Genome Shotgun) chromosome-scale assembly with high contiguity (contig L50 214.2 kb), large portions of mapped sequences (96%) and high base accuracy (99...
March 11, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28276455/corrigendum-the-green-peach-aphid-myzus-persicae-perform-better-on-pre-infested-chinese-cabbage-brassica-pekinensis-by-enhancing-host-plant-nutritional-quality
#12
He-He Cao, Hui-Ru Liu, Zhan-Feng Zhang, Tong-Xian Liu
No abstract text is available yet for this article.
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28275772/rapidly-accessible-click-rotaxanes-utilizing-a-single-amide-hydrogen-bond-templating-motif
#13
Beth E Fletcher, Michael J G Peach, Nicholas H Evans
The synthesis of hydrogen bond templated rotaxanes using the CuAAC click reaction has been achieved in yields of up to 47%, employing near stoichiometric equivalents of macrocycle and readily prepared azide and alkyne half-axle components. Interlocked structure formation has been confirmed by NMR spectroscopy and mass spectrometry. Density functional theory calculations support (1)H NMR spectroscopic analysis that the macrocycle resides over the amide of the axle component, rather than the newly formed triazole, as a result of more favourable hydrogen bond interactions...
March 9, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28262385/genetic-analysis-identifies-the-region-of-origin-of-smuggled-peach-palm-seeds
#14
Michelly Cristo-Araújo, David Bronze Molles, Doriane Picanço Rodrigues, Charles R Clement
Seeds of a plant, supposedly a palm tree known popularly as peach palm (Bactris gasipaes), were seized by the Federal Police in the state of Pará, Brazil, without documentation of legal origin to authorize transportation and marketing in Brazil. They were alleged to be from the western part of Amazonas, Brazil, near the frontier with Peru and Colombia, justifying the lack of documentation. The species was confirmed to be peach palm. To determine the likely place of origin, a genetic analysis was performed to determine the relationship between the seized seeds and representative populations of peach palm from all of Amazonia, maintained in the Peach palm Core Collection, at the National Research Institute for Amazonia, using nine microsatellite loci...
February 20, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28261260/induction-of-systemic-resistance-against-aphids-by-endophytic-bacillus-velezensis-yc7010-via-expressing-phytoalexin-deficient4-in-arabidopsis
#15
Md Harun-Or- Rashid, Ajmal Khan, Mohammad T Hossain, Young R Chung
Aphids are the most destructive insect pests. They suck the sap and transmit plant viruses, causing widespread yield loss of many crops. A multifunctional endophytic bacterial strain Bacillus velezensis YC7010 has been found to induce systemic resistance against bacterial and fungal pathogens of rice. However, its activity against insects attack and underlying cellular and molecular defense mechanisms are not elucidated yet. Here, we show that root drenching of Arabidopsis seedlings with B. velezensis YC7010 can induce systemic resistance against green peach aphid (GPA), Myzus persicae...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28253208/neuropathic-pain-and-quality-of-life-after-wide-local-excision-and-sentinel-lymph-node-biopsy-for-melanoma-a-multicentre-study
#16
Collette H Thomson, Oliver Cassell, Howard Peach, Samantha Holloway, Jennifer Garioch, Marc Moncrieff
Wide local excision and sentinel lymph node biopsy is the mainstay of treatment for patients with melanoma. As survival outcomes improve, longer term quality of life questions become more pertinent and this study aims to assess the factors which may play a role following surgery. A total of, 221 patients who underwent wide local excision and sentinel lymph node biopsy for melanoma (AJCC stage I and II) were recruited from three UK centres. These patients completed a patient outcome questionnaire, which included demographic and treatment data as well as quality of life and pain questionnaires...
April 2017: Melanoma Research
https://www.readbyqxmd.com/read/28252802/pru-p-3-a-marker-allergen-for-lipid-transfer-protein-ltp-sensitization-also-in-central-europe
#17
Nadine Mothes-Luksch, Marianne Raith, Georg Stingl, Margarete Focke-Tejkl, Ebrahim Razzazi-Fazeli, Rene Zieglmayer, Stefan Wöhrl, Ines Swoboda
In the Mediterranean area, lipid transfer proteins (LTPs) are important causes of plant food allergies often associated with severe allergic reactions. There, peach LTP (Pru p 3) seems to be the primary sensitizer, whereas in Central Europe little is known about the importance of LTP-sensitization. In this region allergen extract based diagnosis is often complicated by co-sensitization to Bet v 1, the major birch pollen allergen, its cross-reactive food allergens and profilins. We investigated the role of LTP-sensitization in Central European patients displaying strong allergic reactions to plant-derived food...
March 2, 2017: Allergy
https://www.readbyqxmd.com/read/28249298/the-modern-japanese-color-lexicon
#18
Ichiro Kuriki, Ryan Lange, Yumiko Muto, Angela M Brown, Kazuho Fukuda, Rumi Tokunaga, Delwin T Lindsey, Keiji Uchikawa, Satoshi Shioiri
Despite numerous prior studies, important questions about the Japanese color lexicon persist, particularly about the number of Japanese basic color terms and their deployment across color space. Here, 57 native Japanese speakers provided monolexemic terms for 320 chromatic and 10 achromatic Munsell color samples. Through k-means cluster analysis we revealed 16 statistically distinct Japanese chromatic categories. These included eight chromatic basic color terms (aka/red, ki/yellow, midori/green, ao/blue, pink, orange, cha/brown, and murasaki/purple) plus eight additional terms: mizu ("water")/light blue, hada ("skin tone")/peach, kon ("indigo")/dark blue, matcha ("green tea")/yellow-green, enji/maroon, oudo ("sand or mud")/mustard, yamabuki ("globeflower")/gold, and cream...
March 1, 2017: Journal of Vision
https://www.readbyqxmd.com/read/28244594/unraveling-early-events-in-the-taphrina-deformans-prunus-persica-interaction-an-insight-into-the-differential-responses-in-resistant-and-susceptible-genotypes
#19
Laura A Svetaz, Claudia A Bustamante, Camila Goldy, Nery Rivero, Gabriela L Müller, Gabriel H Valentini, Alisdair R Fernie, María F Drincovich, María V Lara
Leaf peach curl is a devastating disease affecting leaves, flowers and fruits caused by the dimorphic fungus Taphrina deformans. To gain insight into the mechanisms of fungus pathogenesis and plant responses, leaves of a resistant and two susceptible P. persica genotypes were inoculated with blastospores (yeast), and the infection was monitored during 120 hpi. Fungal dimorphism to the filamentous form and induction of ROS, callose synthesis, cell death, and defense compound production was observed independently of the genotype...
February 28, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28242420/biocontrol-of-monilinia-laxa-by-aureobasidium-pullulans-strains-insights-on-competition-for-nutrients-and-space
#20
Alessandra Di Francesco, Luisa Ugolini, Salvatore D'Aquino, Eleonora Pagnotta, Marta Mari
Two Aureobasidium pullulans strains (L1 and L8), able to prevent postharvest fruit decay, were evaluated in order to elucidate how the competition for nutrients and space was involved in their activity against Monilinia laxa, the causal agent of peach brown rot. The competition for nutrients was studied by co-culturing pathogen conidia and antagonists in different conditions of nutrient availability and avoiding contact between them. Both antagonists prevented the germination of conidia of M. laxa in water, reducing germination rate by >35%...
February 17, 2017: International Journal of Food Microbiology
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