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https://www.readbyqxmd.com/read/28813678/therapeutic-antibodies-to-ganglioside-gd2-evolved-from-highly-selective-germline-antibodies
#1
Eric Sterner, Megan L Peach, Marc C Nicklaus, Jeffrey C Gildersleeve
Antibodies play a crucial role in host defense and are indispensable research tools, diagnostics, and therapeutics. Antibody generation involves binding of genomically encoded germline antibodies followed by somatic hypermutation and in vivo selection to obtain antibodies with high affinity and selectivity. Understanding this process is critical for developing monoclonal antibodies, designing effective vaccines, and understanding autoantibody formation. Prior studies have found that antibodies to haptens, peptides, and proteins evolve from polyspecific germline antibodies...
August 15, 2017: Cell Reports
https://www.readbyqxmd.com/read/28801775/plasticizers-and-bisphenol-a-in-packaged-foods-sold-in-the-tunisian-markets-study-of-their-acute-in-vivo-toxicity-and-their-environmental-fate
#2
Asma Beltifa, Anouar Feriani, Monia Machreki, Asma Ghorbel, Lakhdar Ghazouani, Giuseppa Di Bella, Joris Van Loco, Tim Reyns, Hedi Ben Mansour
Today, processed and packaged foods are considered as among the major sources of human exposure to plasticizers and bisphenol which migrate from plastic packing. In the present study, a wide range of food products sold on the Tunisian market such as grain and grain products, milk and dairy products, fats and oil, drink, fish, and sweets have been analyzed firstly in order to identify the presence of phthalates and bisphenol. Then, the identified chemical molecules were studied for their environmental fate and tested in vivo for its toxicity in mice models...
August 12, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28800823/detection-and-quantification-of-alicyclobacillus-acidoterrestris-by-electrical-impedance-in-apple-juice
#3
Pilar Fernández, José Antonio Gabaldón, Mª Jesús Periago
Alicyclobacillus acidoterrestris is a thermotolerant bacterium able to grow in fruit juices and drinks, as the spoilage by Alicyclobacillus in the final product does not product any gas, but leads to a "medicine flavor" due to the formation of guaicol. Also, its detection is a challenge for the quality control departments, because it takes several days to get the results of traditional microbiology methods. This study aimed at developing a more accurate electrical impedance technique for the detection of A...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28794462/fear-of-predation-alters-clone-specific-performance-in-phloem-feeding-prey
#4
Mouhammad Shadi Khudr, Oksana Y Buzhdygan, Jana S Petermann, Susanne Wurst
Fear of predation has been shown to affect prey fitness and behaviour, however, to date little is known about the underlying genetics of responses to predator-associated risk. In an effort to fill this gap we exposed four naïve clones of green peach aphid (Myzus persicae), maintained on the model crop Brassica oleracea, to different types of cues from aphid lion (Chrysoperla carnea). The respective predation risks, we termed Fear Factors, were either lethal (consumption by predator), or non-lethal (non-consumptive predator-associated cues: plant-tethered predator cadavers and homogenised shoot-sprayed or soil-infused blends of predator remains)...
August 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28783871/cardiac-safety-of-ozanimod-a-novel-sphingosine-1-phosphate-receptor-modulator-results-of-a-thorough-qt-qtc-study
#5
Jonathan Q Tran, Jeffrey P Hartung, Allan D Olson, Boaz Mendzelevski, Gregg A Timony, Marcus F Boehm, Robert J Peach, Sheila Gujrathi, Paul A Frohna
Ozanimod is a novel, selective, oral sphingosine-1-phosphate (1 and 5) receptor modulator in development for multiple sclerosis and inflammatory bowel disease. This randomized, double-blind, placebo-controlled, positive-controlled, parallel-group thorough QT study characterized the effects of ozanimod on cardiac repolarization in healthy subjects. Eligible subjects were randomized to 1 of 2 groups: ozanimod (escalated from 0.25 to 2 mg over 14 days) or placebo (for 14 days). A single dose of moxifloxacin 400 mg or placebo was administered on days 2 and 17...
August 7, 2017: Clinical Pharmacology in Drug Development
https://www.readbyqxmd.com/read/28782634/a-new-allergen-family-involved-in-pollen-food-associated-syndrome-snakin-gibberellin-regulated-proteins
#6
Hélène Sénéchal, Jiří Šantrůček, Magdalena Melčová, Petr Svoboda, Jarmila Zídková, Denis Charpin, Laurence Guilloux, Youcef Shahali, Marie-Ange Selva, Rémy Couderc, Tomoyasu Aizawa, Pascal Poncet
At least two pollen food associated syndromes were described with cypress pollen allergy involving peach and citrus. Snakin/gibberellin regulated proteins are described herein to be the cross-reactive allergens between Cupressus sempervirens pollen and fruit/vegetables.
August 3, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28769956/rosaceae-fruit-development-ripening-and-post-harvest-an-epigenetic-perspective
#7
REVIEW
Silvia Farinati, Angela Rasori, Serena Varotto, Claudio Bonghi
Rosaceae is a family with an extraordinary spectrum of fruit types, including fleshy peach, apple, and strawberry that provide unique contributions to a healthy diet for consumers, and represent an excellent model for studying fruit patterning and development. In recent years, many efforts have been made to unravel regulatory mechanism underlying the hormonal, transcriptomic, proteomic and metabolomic changes occurring during Rosaceae fruit development. More recently, several studies on fleshy (tomato) and dry (Arabidopsis) fruit model have contributed to a better understanding of epigenetic mechanisms underlying important heritable crop traits, such as ripening and stress response...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28762777/occurrence-and-health-risk-of-patulin-and-pyrethroids-in-fruit-juices-consumed-in-bangkok-thailand
#8
Saranya Poapolathep, Phanwimol Tanhan, Onuma Piasai, Kanjana Imsilp, Jana Hajslova, Mario Giorgi, Susumu Kumagai, Amnart Poapolathep
The mycotoxin patulin (PAT) is well known as a natural contaminant of apple- and other fruit-based products. Pesticides are a group of chemicals abundantly used in agriculture to maximize productivity by protecting crops from pests and weeds. Because of their harmful health effects, PAT and pesticides are strictly monitored. The current study was undertaken to investigate the significance of PAT and pyrethroid insecticide contamination in a variety of fruit juices in Bangkok. To do this, a total of 200 fruit juice samples, consisting of 40 samples each of apple, apricot, peach, pineapple, and grape juice, were collected from supermarkets in Bangkok, Thailand...
September 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28758607/larval-species-composition-and-genetic-structures-of-carposina-sasakii-grapholita-dimorpha-and-grapholita-molesta-from-korea
#9
D H Kwon, H K Kwon, D H Kim, C Y Yang
Rapid determination of the larval species composition and understanding of their genetic structure is important to establish the appropriate management system for multiple species infesting in fruits. We established accurate and rapid diagnostic methods based on multiplex polymerase chain reaction (PCR) diagnostic techniques to discriminate the three major lepidopteran species in orchard, Carposina sasakii, Grapholita dimorpha, and Grapholita molesta. Each species was identified by amplifying species-specific PCR products (375 bp for C...
July 31, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28751691/a-deletion-affecting-an-lrr-rlk-gene-co-segregates-with-the-fruit-flat-shape-trait-in-peach
#10
Elena López-Girona, Yu Zhang, Iban Eduardo, José Ramón Hernández Mora, Konstantinos G Alexiou, Pere Arús, María José Aranzana
In peach, the flat phenotype is caused by a partially dominant allele in heterozygosis (Ss), fruits from homozygous trees (SS) abort a few weeks after fruit setting. Previous research has identified a SSR marker (UDP98-412) highly associated with the trait, found suitable for marker assisted selection (MAS). Here we report a ∼10 Kb deletion affecting the gene PRUPE.6G281100, 400 Kb upstream of UDP98-412, co-segregating with the trait. This gene is a leucine-rich repeat receptor-like kinase (LRR-RLK) orthologous to the Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) group...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28734042/multivariate-optimization-of-conditions-for-digestion-of-wet-feeds-for-dogs-and-cats-using-a-closed-digester-block-and-multielement-determination-by-icp-oes
#11
Dayara Virgínia Lino Ávila, Sidnei Oliveira Souza, Silvlvânio Silvlvério Lopes Costa, Carlos Alexandre Borges Garcia, José do Patrocínio Hora Alvlves, Rennan Geovanny Oliveira Araujo, Elisangela Andrade Passos
A full 2⁴ factorial design was applied to find the best combination of diluted reagents (HNO₃ and H₂O₂), time, and temperature for the digestion of samples of wet feed for dogs and cats using a closed digestion block. The residual carbon concentration (RCC) was used as the response in the factorial design. All variables and their interactions significantly influenced the digestion of the feed samples, as indicated by the RCC. The conditions established for the digestion of 0.05 g (dry mass) wet feed samples were the addition of 3...
July 21, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28729739/host-plants-and-obligate-endosymbionts-are-not-the-sources-for-biosynthesis-of-the-aphid-alarm-pheromone
#12
Zhi-Juan Sun, Zheng-Xi Li
(E)-β-farnesene (EβF) is the major component of the alarm pheromone of many aphid species, but where EβF is synthesized in aphids is only partly understood. There are at least three most possible sources for the alarm pheromone: host plants, aphid obligate endosymbiont and aphids themselves. Here we eliminated the possibility of host plants and the obligate endosymbiont Buchnera aphidicola as the sources for EβF released by aphids. We excluded the possible effects of host plants on EβF biosynthesis by rearing aphids on non-plant diets...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28726882/trimethylsilyl-hedgehogs-a-novel-class-of-super-efficient-hydrocarbon-surfactants
#13
Adam Czajka, Christopher Hill, Jocelyn Peach, Jonathan C Pegg, Isabelle Grillo, Frédéric Guittard, Sarah E Rogers, Masanobu Sagisaka, Julian Eastoe
Presented here are the results for a novel class of hydrocarbon surfactants, termed trimethylsilyl hedgehogs (TMS-hedgehogs), due to the presence of silicon in the tails. By comparing the surface properties of these hybrid hedgehogs to purely hydrocarbon equivalents, links between performance and the structure are made. Namely, by controlling the molecular volume of the surfactant fragments, improvements can be made in surface coverage, generating lower surface energy monolayers. Small-angle neutron scattering (SANS) data have been collected showing that these novel surfactants aggregate to form ellipsoidal micelles which grow with increasing concentration...
July 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28722114/uv-b-irradiation-differentially-regulates-terpene-synthases-and-terpene-content-of-peach
#14
Hongru Liu, Xiangmei Cao, Xiaohong Liu, Rui Xin, Jiaojiao Wang, Jie Gao, Boping Wu, Liuxiao Gao, Changjie Xu, Bo Zhang, Donald Grierson, Kunsong Chen
Plants generate protective molecules in response to UV light. In laboratory experiments, 48 h UV-B irradiation of peach fruit and leaves reduced the flavor-related monoterpene linalool by 60 %. No isoprene was detected but other terpenoids increased significantly, including a 3-fold accumulation of the sesquiterpene (E,E)-α-farnesene, which was also increased by jasmonic acid treatment. RNA-sequencing revealed altered transcript levels for two terpene synthases: PpTPS1, a TPS-g subfamily member, decreased by 86 % and PpTPS2, a TPS-b subfamily member, increased 80-fold...
July 19, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28720943/non-enzymatic-browning-due-to-storage-is-reduced-by-using-clarified-lemon-juice-as-acidifier-in-industrial-scale-production-of-canned-peach-halves
#15
Domingo Saura, Salud Vegara, Nuria Martí, Manuel Valero, José Laencina
Non-enzymatic browning (NEB) in canned peach halves in syrup during storage was investigated. Absorbance at 420 nm (A420), colorimetric parameters (CIELab, TCD and La/b), fructose, glucose and sucrose, total sugar, organic acids, ascorbic acid (AA), dehydroascorbic acid, and 2,3-diketogulonic acid were used to estimate the extent of NEB during 1 year of storage at 30 °C and the relationships between each of these parameters and A420 were established. The investigation was carried out to explore the possibility of replacing the E330 commonly used as acidifier by turbid or clarified lemon juice (TLJ or CLJ) to obtain a product having good nutrition with better retention of quality...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28718970/activation-of-semantic-information-at-the-sublexical-level-during-handwriting-production-evidence-from-inhibition-effects-of-chinese-semantic-radicals-in-the-picture-word-interference-paradigm
#16
Xuqian Chen, Yuanlan Liao, Xianzhe Chen
Using a non-alphabetic language (e.g., Chinese), the present study tested a novel view that semantic information at the sublexical level should be activated during handwriting production. Over 80% of Chinese characters are phonograms, in which semantic radicals represent category information (e.g., 'chair,' 'peach,' 'orange' are related to plants) while phonetic radicals represent phonetic information (e.g., 'wolf,' 'brightness,' 'male,' are all pronounced /lang/). Under different semantic category conditions at the lexical level (semantically related in Experiment 1; semantically unrelated in Experiment 2), the orthographic relatedness and semantic relatedness of semantic radicals in the picture name and its distractor were manipulated under different SOAs (i...
August 2017: Scandinavian Journal of Psychology
https://www.readbyqxmd.com/read/28712832/enhanced-pru-p-3-ige-binding-activity-by-selective-free-fatty-acid-interaction
#17
Pawel Dubiela, Roberta Aina, Dominika Polak, Sabine Geiselhart, Piotr Humeniuk, Barbara Bohle, Stefano Alessandri, Rebecca Del Conte, Francesca Cantini, Tomasz Borowski, Merima Bublin, Karin Hoffmann-Sommergruber
Upon binding to certain lipids, the major peach allergen, Pru p 3, undergoes conformational changes thus exposing one dominant IgE epitope. This conformational change enhances the allergenicity of Pru p 3.
July 14, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28712341/genetic-analysis-of-bactrocera-zonata-diptera-tephritidae-populations-from-india-based-on-cox1-and-nad1-gene-sequences
#18
Jaipal S Choudhary, Naiyar Naaz, Moanaro Lemtur, Bikash Das, Arun Kumar Singh, Bhagwati P Bhatt, Chandra S Prabhakar
The peach fruit fly, Bactrocera zonata, is among the most serious and polyphagous insect pest of fruit crops in many parts of the world under genus Bactrocera. In the present study, the genetic structure, diversity and demographic history of B. zonata in India were inferred from mitochondrial cytochrome oxidase 1 (cox1) and NADH dehydrogenase 1 (nad1) sequences. The efficiency of DNA barcodes for identification of B. zonata was also tested. Genetic diversity indices [number of haplotypes (H), haplotype diversity (Hd), nucleotide diversity (π) and average number of nucleotide differences (k)] of B...
July 15, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28685994/gibberellin-regulated-protein-in-japanese-apricot-is-an-allergen-cross-reactive-to-pru-p-7
#19
Naoko Inomata, Mami Miyakawa, Michiko Aihara
BACKGROUND: Gibberellin-regulated protein (GRP, also known as peamaclein) was recently identified as a new marker allergen related to systemic reactions in peach allergy; however, its role in other fruit allergies is unknown. OBJECTIVE: To investigate the allergenicity of Japanese apricot (JA) GRP and clarify the clinical characteristics of JA allergy patients with GRP sensitization. METHODS: Seven patients (two males, five females, mean age 28...
July 6, 2017: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/28672765/discovery-and-annotation-of-plant-endogenous-target-mimicry-sequences-from-public-transcriptome-libraries-a-case-study-of-prunus-persica
#20
Gökhan Karakülah
Novel transcript discovery through RNA sequencing has substantially improved our understanding of the transcriptome dynamics of biological systems. Endogenous target mimicry (eTM) transcripts, a novel class of regulatory molecules, bind to their target microRNAs (miRNAs) by base pairing and block their biological activity. The objective of this study was to provide a computational analysis framework for the prediction of putative eTM sequences in plants, and as an example, to discover previously un-annotated eTMs in Prunus persica (peach) transcriptome...
June 28, 2017: Journal of Integrative Bioinformatics
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