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mesenchymal stromal cells

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https://www.readbyqxmd.com/read/28748151/mesenchymal-stromal-cell-therapy-as-treatment-for-ischemic-heart-failure-the-msc-hf-study
#1
EDITORIAL
Enca Martin-Rendon, Mariann Gyöngyösi
No abstract text is available yet for this article.
June 2017: Cardiovascular Diagnosis and Therapy
https://www.readbyqxmd.com/read/28747783/engineering-the-geometrical-shape-of-mesenchymal-stromal-cells-through-defined-cyclic-stretch-regimens
#2
Brandan Walters, Tatiana Uynuk-Ool, Miriam Rothdiener, Julian Palm, Melanie L Hart, Jan P Stegemann, Bernd Rolauffs
Stem cells have been predicted to improve disease outcomes and patient lives. Steering stem cell fate - through controlling cell shape - may substantially accelerate progress towards this goal. As mesenchymal stromal cells (MSCs) are continuously exposed in vivo to a dynamically changing biomechanical environment, we hypothesized that exogenous forces can be applied for engineering a variety of significantly different MSC shapes. We applied specific cyclic stretch regimens to human MSCs and quantitatively measured the resulting cell shape, alignment, and expression of smooth muscle (SMC) differentiation markers, as those have been associated with elongated morphology...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28747655/strategies-to-mitigate-variability-in-engineering-human-nasal-cartilage
#3
Stephen H J Andrews, Melanie Kunze, Aillette Mulet-Sierra, Lynn Williams, Khalid Ansari, Martin Osswald, Adetola B Adesida
Skin cancer and its associated treitments can have devastating consequences for survivors; this is particularly true when cancer occurs on the nose. Recent work has applied cell-based tissue engineering (TE) strategies to develop nasal cartilage constructs for reconstruction of the nose. In this study, we have generated human nasal cartilage on a clinically approved collagen scaffold to investigate the donor-to-donor variability of TE cartilage and evaluated strategies to mitigate it. We also evaluated the gene expression of the family of fibroblast growth factor receptors (FGFR1-4) and their association with tissue quality...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28747098/effect-of-mesenchymal-stromal-cells-on-t-cells-in-septic-context-immuno-suppression-or-immuno-stimulation
#4
Sebastien Le Burel, Cédric Thépenier, Laetitia Boutin, Jean-Jacques Lataillade, Juliette Peltzer
Sepsis is a complex process including a first wave of damage partially due to the body's response to pathogens, followed by a phase of immune cell dysfunction. The efficacy of a pharmacological approach facing a rapidly evolving system implies a perfect timing of administration, this difficulty could explain recent failure of clinical trials. MSC are usually defined as immunosuppressive, and their beneficial effects in preclinical models of acute sepsis have been shown to rely partly on such ability. If non regulated, this phenotype could be harmful in the immunosuppressed context arising hours after sepsis onset...
July 26, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28745329/involvement-of-maf-spp1-axis-in-the-development-of-bone-marrow-fibrosis-in-pmf-patients
#5
S Ruberti, E Bianchi, P Guglielmelli, S Rontauroli, G Barbieri, L Tavernari, T Fanelli, R Norfo, V Pennucci, G C Fattori, C Mannarelli, N Bartalucci, B Mora, L Elli, M A Avanzini, C Rossi, S Salmoiraghi, R Zini, S Salati, Z Prudente, V Rosti, F Passamonti, A Rambaldi, S Ferrari, E Tagliafico, A M Vannucchi, R Manfredini
Primary Myelofibrosis (PMF) is a myeloproliferative neoplasm characterized by hyperplastic megakaryopoiesis and myelofibrosis. We recently described the upregulation of MAF in PMF CD34+ hematopoietic progenitor cells (HPCs) compared to healthy donor. Here we demonstrated that MAF is also upregulated in PMF compared to Essential Thrombocytemia (ET) and Polycytemia Vera (PV) HPCs. MAF overexpression and knockdown experiments shed some light into the role of MAF in PMF pathogenesis, by demonstrating that MAF favours the megakaryocyte and monocyte/macrophage commitment of HPCs and leads to the increased expression of proinflammatory and profibrotic mediators...
July 12, 2017: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/28743889/limbal-stromal-cells-derived-from-porcine-tissue-demonstrate-mesenchymal-characteristics-in-vitro
#6
Julia Fernández-Pérez, Marcus Binner, Carsten Werner, Laura J Bray
Limbal stromal cells (LSCs) from the human ocular surface display mesenchymal stromal cell characteristics in vitro. In this study, we isolated cells from the porcine limbal stroma (pLSCs), characterised them, and evaluated their ability to support angiogenesis and the culture of porcine limbal epithelial stem cells (pLESCs). The isolated cells adhered to plastic and grew in monolayers in vitro using serum-supplemented or serum-free medium. The pLSCs demonstrated expression of CD29, and cross-reactivity with anti-human CD45, CD90, CD105, CD146, and HLA-ABC...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28743464/stem-cell-therapy-for-abrogating-stroke-induced-neuroinflammation-and-relevant-secondary-cell-death-mechanisms
#7
REVIEW
Connor Stonesifer, Sydney Corey, Shaila Ghanekar, Zachary Diamandis, Sandra A Acosta, Cesar V Borlongan
Ischemic stroke is a leading cause of death worldwide. A key secondary cell death mechanism mediating neurological damage following the initial episode of ischemic stroke is the upregulation of endogenous neuroinflammatory processes to levels that destroy hypoxic tissue local to the area of insult, induce apoptosis, and initiate a feedback loop of inflammatory cascades that can expand the region of damage. Stem cell therapy has emerged as an experimental treatment for stroke, and accumulating evidence supports the therapeutic efficacy of stem cells to abrogate stroke-induced inflammation...
July 22, 2017: Progress in Neurobiology
https://www.readbyqxmd.com/read/28740515/in-vivo-tracking-of-chemokine-receptor-cxcr4-engineered-mesenchymal-stem-cell-migration-by-optical-molecular-imaging
#8
Senthilkumar Kalimuthu, Ji Min Oh, Prakash Gangadaran, Liya Zhu, Ho Won Lee, Ramya Lakshmi Rajendran, Se Hwan Baek, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn
CXCR4, the stromal cell-derived factor-1 receptor, plays an important role in the migration of hematopoietic progenitor/stem cells to injured and inflamed areas. Noninvasive cell tracking methods could be useful for monitoring cell fate. Therefore, in this study, we evaluated the efficacy of an intravenous infusion of genetically engineered mesenchymal stem cells (MSCs) overexpressing CXC chemokine receptor 4 (CXCR4) to home to the tumor, by optical imaging. We constructed a retroviral vector containing CXCR with dual reporter genes, eGFP and Fluc2, under the control of an EF1α promoter (pBABE-EF1α-CXCR4-eGFP-IRES-Fluc2)...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28740511/safety-and-tolerance-of-donor-derived-mesenchymal-stem-cells-in-pediatric-living-donor-liver-transplantation-the-mystep1-study
#9
Steffen Hartleif, Michael Schumm, Michaela Döring, Markus Mezger, Peter Lang, Marc H Dahlke, Joachim Riethmüller, Alfred Königsrainer, Rupert Handgretinger, Silvio Nadalin, Ekkehard Sturm
BACKGROUND: Calcineurin inhibitors (CNI) have significantly improved patient and graft survival in pediatric liver transplantation (pLT). However, CNI toxicity leads to significant morbidity. Moreover, CNIs cannot prevent long-term allograft injury. Mesenchymal stem (stromal) cells (MSC) have potent immunomodulatory properties, which may promote allograft tolerance and ameliorate toxicity of high-dose CNI. The MYSTEP1 trial aims to investigate safety and feasibility of donor-derived MSCs in pLT...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28739546/in-situ-tissue-regeneration-through-sdf-1%C3%AE-driven-cell-recruitment-and-stiffness-mediated-bone-regeneration-in-a-critical-sized-segmental-femoral-defect
#10
Amaia Cipitria, Kathrin Boettcher, Sophia Schoenhals, Daniela S Garske, Katharina Schmidt-Bleek, Agnes Ellinghaus, Anke Dienelt, Anja Peters, Manav Mehta, Christopher M Madl, Nathaniel Huebsch, David J Mooney, Georg N Duda
In-situ tissue regeneration aims to utilize the body's endogenous healing capacity through the recruitment of host stem or progenitor cells to an injury site. Stromal cell-derived factor-1 α (SDF-1 α) is widely discussed as a potent chemoattractant. Here we use a cell-free biomaterial-based approach to (i) deliver SDF-1 α for the recruitment of endogenous bone marrow-derived stromal cells (BMSC) into a critical-sized segmental femoral defect in rats and to (ii) induce hydrogel stiffness-mediated osteogenic differentiation in-vivo...
July 21, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28739167/establishment-of-nf-%C3%AE%C2%BAb-sensing-and-interleukin-4-secreting-mesenchymal-stromal-cells-as-an-on-demand-drug-delivery-system-to-modulate-inflammation
#11
Tzuhua Lin, Jukka Pajarinen, Akira Nabeshima, Laura Lu, Karthik Nathan, Zhenyu Yao, Stuart B Goodman
Chronic inflammation is associated with up-regulation of the transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and excessive inflammatory cytokine secretion by M1 macrophages. The anti-inflammatory cytokine interleukin (IL)-4 converts pro-inflammatory M1 macrophages into an anti-inflammatory and tissue-regenerative M2 phenotype, thus reducing inflammation and enhancing tissue regeneration. We have generated NF-κB responsive, or constitutively active IL-4 expression lentiviral vectors transduced into murine bone marrow-derived mesenchymal stromal cells (MSCs)...
July 21, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28737959/influence-of-patient-related-factors-on-number-of-mesenchymal-stromal-cells-reached-after-in-vitro-culture-expansion-for-clinical-treatment
#12
Abbas Ali Qayyum, Kamal Preet Kaur, Anders Bruun Mathiasen, Mandana Haack-Sørensen, Annette Ekblond, Jens Kastrup
BACKGROUND: Number of stromal cells injected in patients with ischaemic heart disease (IHD) may be of importance for the treatment efficacy, which in turn may be influenced by various patient-related factors. In this study, we investigate whether patient-related factors influence the number of autologous stromal cells reached after in vitro culture expansion for clinical therapy. METHODS: Culture expansion data from 111 patients with IHD treated with autologous stromal cells in three clinical trials were used...
July 24, 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/28736264/mesenchymal-stem-stromal-cell-extracellular-vesicles-from-active-principle-to-next-generation-drug-delivery-system
#13
REVIEW
Barbara Crivelli, Theodora Chlapanidas, Sara Perteghella, Enrico Lucarelli, Luisa Pascucci, Anna Teresa Brini, Ivana Ferrero, Mario Marazzi, Augusto Pessina, Maria Luisa Torre
It has been demonstrated that the biological effector of mesenchymal stem/stromal cells (MSCs) is their secretome, which is composed of a heterogeneous pool of bioactive molecules, partially enclosed in extracellular vesicles (EVs). Therefore, the MSC secretome (including EVs) has been recently proposed as possible alternative to MSC therapy. The secretome can be considered as a protein-based biotechnological product, it is probably safer compared with living/cycling cells, it presents virtually lower tumorigenic risk, and it can be handled, stored and sterilized as an Active Pharmaceutical/Principle Ingredient (API)...
July 20, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28735502/regenerative-medicine-in-kidney-disease-where-we-stand-and-where-to-go
#14
REVIEW
Fernanda T Borges, Nestor Schor
The kidney is a complex organ with more than 20 types of specialized cells that play an important role in maintaining the body's homeostasis. The epithelial tubular cell is formed during embryonic development and has little proliferative capacity under physiological conditions, but after acute injury the kidney does have regenerative capacity. However, after repetitive or severe lesions, it may undergo a maladaptation process that predisposes it to chronic kidney injury. Regenerative medicine includes various repair and regeneration techniques, and these have gained increasing attention in the scientific literature...
July 22, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28734802/co-transplantation-of-mesenchymal-and-neural-stem-cells-and-overexpressing-stromal-derived-factor-1-for-treating-spinal-cord-injury
#15
Andrew N Stewart, Griffin Kendziorski, Zachary M Deak, Dara J Brown, Matthew N Fini, Katherine L Copely, Julien Rossignol, Gary L Dunbar
Genetic engineering of mesenchymal stem cells (MSCs) and neuronal stem cells (NSCs) has been used to treat spinal cord injuries (SCI). As a mechanism of therapy, MSCs secrete high amounts of anti-inflammatory cytokines and trophic factors, while NSCs can differentiate into neuronal lineages and aid in tissue replacement. Additionally, the forced overexpression of secreted proteins can enhance the secretome of transplanted cells, which can increase therapeutic efficacy. This study utilized a combinational treatment consisting of MSCs, NSCs, and the forced overexpression of the chemokine stromal-derived factor-1 (SDF-1) from MSCs (SDF-1-MSCs), to treat a rat model of SCI...
July 19, 2017: Brain Research
https://www.readbyqxmd.com/read/28734679/assessment-of-biodistribution-using-mesenchymal-stromal-cells-algorithm-for-study-design-and-challenges-in-detection-methodologies
#16
Blanca Reyes, Maria Isabel Coca, Margarita Codinach, María Dolores López-Lucas, Anna Del Mazo-Barbara, Marta Caminal, Irene Oliver-Vila, Valentín Cabañas, Silvia Lope-Piedrafita, Joan García-López, José M Moraleda, Cesar G Fontecha, Joaquim Vives
BACKGROUND AIMS: Biodistribution of candidate cell-based therapeutics is a critical safety concern that must be addressed in the preclinical development program. We aimed to design a decision tree based on a series of studies included in actual dossiers approved by competent regulatory authorities, noting that the design, execution and interpretation of pharmacokinetics studies using this type of therapy is not straightforward and presents a challenge for both developers and regulators...
July 19, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28734678/improvement-of-adipose-tissue-derived-cells-by-low-energy-extracorporeal-shock-wave-therapy
#17
Eleni Priglinger, Christina M A P Schuh, Carolin Steffenhagen, Christoph Wurzer, Julia Maier, Sylvia Nuernberger, Wolfgang Holnthoner, Christiane Fuchs, Susanne Suessner, Dominik Rünzler, Heinz Redl, Susanne Wolbank
BACKGROUND: Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency...
July 19, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28733800/steroid-mediated-decrease-in-blood-mesenchymal-stem-cells-in-liver-transplant-could-impact-long-term-recovery
#18
Nykia D Walker, Yasmine Mourad, Katherine Liu, Michael Buxhoeveden, Catherine Schoenberg, Jean D Eloy, Dorian J Wilson, Lloyd G Brown, Andrei Botea, Faraz Chaudhry, Steven J Greco, Nicholas M Ponzio, Nikolaos Pyrsopoulos, Baburao Koneru, Yuriy Gubenko, Pranela Rameshwar
Orthotopic liver transplant (OLT) remains the standard of care for end stage liver disease. To circumvent allo-rejection, OLT subjects receive gluococorticoids (GC). We investigated the effects of GC on endogenous mesenchymal stem (stromal) cells (MSCs) in OLT. This question is relevant because MSCs have regenerative potential and immune suppressor function. Phenotypic analyses of blood samples from 12 OLT recipients, at pre-anhepatic, anhepatic and post-transplant (2 h, Days 1 and 5) indicated a significant decrease in MSCs after GC injection...
July 21, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28732530/minocycline-modulates-nf%C3%AE%C2%BAb-phosphorylation-and-enhances-antimicrobial-activity-against-staphylococcus-aureus-in-mesenchymal-stromal-stem-cells
#19
Alberto Daniel Guerra, Warren E Rose, Peiman Hematti, W John Kao
BACKGROUND: Mesenchymal stromal/stem cells (MSCs) have demonstrated pro-healing properties due to their anti-inflammatory, angiogenic, and even antibacterial properties. We have shown previously that minocycline enhances the wound healing phenotype of MSCs, and MSCs encapsulated in poly(ethylene glycol) and gelatin-based hydrogels with minocycline have antibacterial properties against Staphylococcus aureus (SA). Here, we investigated the signaling pathway that minocycline modulates in MSCs which results in their enhanced wound healing phenotype and determined whether preconditioning MSCs with minocycline has an effect on antimicrobial activity...
July 21, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28730782/human-glioma-cells-conditioned-medium-promotes-proliferation-and-migration-of-human-bone-marrow-mesenchymal-stromal-cells-in-vitro
#20
Rusen Zhu, Xinggui Chen, Jinghong Li, Liubo Lan
PURPOSE: To investigate changes in cell proliferation and migration of human bone marrow-derived mesenchymal stromal cells (hMSCs) in glioma environment, in order to assess the tumorigenic risk of hMSCs in the clinical application to treat human gliomas. METHODS: hMSCs were obtained from normal adult persons and identified by their morphological characteristics and test of their stemness. The U251 glioma cell-conditioned medium (U251-CM) was obtained to simulate the human glioma environment in vitro...
May 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
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