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https://www.readbyqxmd.com/read/29668121/lipofection-of-plasmid-dna-into-human-mast-cell-lines-using-lipid-nanoparticles-generated-by-microfluidic-mixing
#1
Brett A Duguay, Kate Wei-Chen Huang, Marianna Kulka
Mast cells are important immune cells that have significant roles in mediating allergy and asthma. Therefore, studying the molecular mechanisms regulating these and other processes in mast cells is important to elucidate. Methods such as lipofection, transduction, and electroporation are often employed to dissect these mechanisms by disrupting gene expression in mast cell lines. However, as with other leukocytes, human mast cells (HMCs) are often refractory to the delivery of plasmids by lipofection. In this study, we investigated the utility of lipid nanoparticles (LNPs) containing the ionizable cationic lipids 1,2-dioleoyloxy-3-dimethylaminopropane, 1,2-dioleyloxy-3-dimethylaminopropane, or 2,2-dilinoleyl-4-(2-dimethylaminoethyl)-[1,3]-dioxolane for the delivery of plasmid DNA into HMC lines...
April 18, 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29668080/control-of-the-ethylene-signaling-pathway-prevents-plant-defenses-during-intracellular-accommodation-of-the-rhizobia
#2
Fathi Berrabah, Thierry Balliau, El Hosseyn Aït-Salem, Jeoffrey George, Michel Zivy, Pascal Ratet, Benjamin Gourion
Massive intracellular populations of symbiotic bacteria, referred to as rhizobia, are housed in legume root nodules. Little is known about the mechanisms preventing the development of defense in these organs although genes such as symCRK and DNF2 of the model legume Medicago truncatula are required for this control after rhizobial internalization in host nodule cells. Here we investigated the molecular basis of the symbiotic control of immunity. Proteomic analysis was performed to compare functional (wild-type) and defending nodules (symCRK)...
April 18, 2018: New Phytologist
https://www.readbyqxmd.com/read/29668079/co-culture-of-adipose-derived-stem-cells-and-chondrocytes-on-three-dimensionally-printed-bioscaffolds-for-craniofacial-cartilage-engineering
#3
Robert J Morrison, Hassan B Nasser, Khaled N Kashlan, David A Zopf, Derek J Milner, Colleen L Flanangan, Matthew B Wheeler, Glenn E Green, Scott J Hollister
OBJECTIVES/HYPOTHESIS: Reconstruction of craniofacial cartilagenous defects are among the most challenging surgical procedures in facial plastic surgery. Bioengineered craniofacial cartilage holds immense potential to surpass current reconstructive options, but limitations to clinical translation exist. We endeavored to determine the viability of utilizing adipose-derived stem cell-chondrocyte co-culture and three-dimensional (3D) printing to produce 3D bioscaffolds for cartilage tissue engineering...
April 18, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29668030/uv-c-irradiation-on-the-quality-of-green-tea-effect-on-catechins-antioxidant-activity-and-cytotoxicity
#4
Matthew J Vergne, Ankit Patras, Manreet S Bhullar, Lincoln M Shade, Michael Sasges, Kanyasiri Rakariyatham, Che Pan, Hang Xiao
Short-wavelength ultraviolet (UV-C) irradiation is a nonthermal processing technique that is a possible alternative to the heat-pasteurization of tea beverages. This study investigated the effect of UV-C irradiation on the polyphenolic and total phenolic contents of a green tea beverage and analyzed cytotoxicity of irradiated green tea using a novel continuous flow-through UV system. UV-C fluence levels ranging from 0 to 240 mJ/cm2 were delivered to green tea, and polyphenols were chemically profiled. Continuous-flow UV-C irradiation of the green tea beverage at a fluence of 68 mJ/cm2  induced a minor reduction in the concentration of the most abundant catechin in green tea, (-)-epigallocatechin gallate (EGCG), from 145 to 131...
April 18, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29668002/a-sharp-edge-based-acoustofluidic-chemical-signal-generator
#5
Po-Hsun Huang, Chung Yu Chan, Peng Li, Yuqi Wang, Nitesh Nama, Hunter Bachman, Tony Jun Huang
Resolving the temporal dynamics of cell signaling pathways is essential for regulating numerous downstream functions, from gene expression to cellular responses. Mapping these signaling pathways requires the exposure of cells to time-varying chemical signals; these are difficult to generate and control over a wide temporal range. Herein, we present an acoustofluidic chemical signal generator based on a sharp-edge-based micromixing strategy. The device, simply by modulating the driving signals of an acoustic transducer including the ON/OFF switching frequency, actuation time and duty cycle, is capable of generating both single-pulse and periodic chemical signals that are temporally controllable in terms of stimulation period, stimulation duration and duty cycle...
April 18, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29667836/molecular-dynamics-simulations-are-redefining-our-view-of-peptides-interacting-with-biological-membranes
#6
Jakob P Ulmschneider, Martin B Ulmschneider
Ever since the first molecular mechanics computer simulations of biological molecules became possible, there has been the dream to study all complex biological phenomena in silico, simply bypassing the enormous experimental challenges and their associated costs. For this, two inherent requirements need to be met: First, the time scales achievable in simulations must reach up to the millisecond range and even longer. Second, the computational model must accurately reproduce what is measured experimentally. Despite some recent successes, the general consensus in the field to date has been that neither of these conditions have yet been met and that the dream will be realized, if at all, only in the distant future...
April 18, 2018: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29667834/phase-transformation-of-geo2-glass-to-nanocrystals-under-ambient-condition
#7
Zhuohao Xiao, Xin-Yuan Sun, Xiuying Li, Yongqing Wang, Zhiqiang Wang, Bowei Zhang, Xianglin Li, Ze Xiang Shen, Lingbing Kong, Yizhong Huang
Theoretically, the accomplishment of phase transformation requires sufficient energy to overcome the barriers of structure rearrangements. Transition of an amorphous to crystalline structure is implemented traditionally by heating at high temperatures. However, phase transformation under ambient condition without involving external energy has not been reported. Here, we demonstrate that phase transformation of GeO2 glass to nanocrystals can be triggered at ambient condition when subjected to aqueous environments...
April 18, 2018: Nano Letters
https://www.readbyqxmd.com/read/29667764/rrm2-promotes-the-progression-of-human-glioblastoma
#8
Chao Li, Jingfang Zheng, Si Chen, Bin Huang, Guosheng Li, Zichao Feng, Jiwei Wang, Shujun Xu
Glioblastoma pathogenesis is related to multiple processes that affected by dozens of regulatory factors, but the potential underlying factors regulating glioblastoma progression remains unclear. The goal of this research was to determine how the ribonucleotide reductase M2 subunit (RRM2) influenced proliferation, invasion, migration, and apoptosis of human glioblastoma cells. The level of proliferation of human glioblastoma cells was measured through CCK8, colony formation assay and immunofluorescence stains...
April 18, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29667761/plant-glycosylphosphatidylinositol-gpi-anchored-proteins-at-the-plasma-membrane-cell-wall-nexus
#9
REVIEW
Trevor H Yeats, Antony Bacic, Kim L Johnson
Approximately 1% of plant proteins are predicted to be post-translationally modified with a glycosylphosphatidylinositol (GPI) anchor that tethers the polypeptide to the outer leaflet of the plasma membrane. While the synthesis and structure of GPI anchors is largely conserved across eukaryotes, the repertoire of functional domains present in the GPI-anchored proteome has diverged substantially. In plants, this includes a large fraction of the GPI-anchored proteome being further modified with plant-specific arabinogalactan (AG) O-glycans...
April 18, 2018: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/29667735/beyond-voltage-gated-ion-channels-voltage-operated-membrane-proteins-and-cellular-processes
#10
REVIEW
Jianping Zhang, Xingjuan Chen, Yucong Xue, Nikita Gamper, Xuan Zhang
Voltage-gated ion channels were believed to be the only voltage-sensitive proteins in excitable (and some non-excitable) cells for a long time. Emerging evidence indicates that the voltage-operated model is shared by some other transmembrane proteins expressed in both excitable and non-excitable cells. In this review, we summarize current knowledge about voltage-operated proteins, which are not classic voltage-gated ion channels as well as the voltage-dependent processes in cells for which single voltage-sensitive proteins have yet to be identified...
April 18, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29667686/rheological-characterization-of-dynamic-remodeling-of-the-pericellular-region-by-human-mesenchymal-stem-cell-secreted-enzymes-in-well-defined-synthetic-hydrogel-scaffolds
#11
Maryam Daviran, Sarah M Longwill, Jonah F Casella, Kelly M Schultz
Human mesenchymal stem cells (hMSCs) dynamically remodel their microenvironment during basic processes, such as migration and differentiation. Migration requires extracellular matrix invasion, necessitating dynamic cell-material interactions. Understanding these interactions is critical to advancing materials designs that harness and manipulate these processes for applications including wound healing and tissue regeneration. In this work, we encapsulate hMSCs in a cell-degradable poly(ethylene glycol)-peptide hydrogel to determine how cell-secreted enzymes, specifically matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), create unique pericellular microenvironments...
April 18, 2018: Soft Matter
https://www.readbyqxmd.com/read/29667552/harvesting-isolation-and-differentiation-of-rat-adipose-derived-stem-cells
#12
Panayiotis D Megaloikonomos, Georgios N Panagopoulos, Myrto Bami, Vasileios G Igoumenou, Leonidas Dimopoulos, Adamantia Milonaki, Maria Kyriakidou, Evanthia Mitsiokapa, Jane Anastassopoulou, Andreas F Mavrogenis
BACKGROUND: Adipose tissue is one of the most attractive source of stem cells because it can be easily harvested and yields a greater stromal cell density. The multi-lineage potential of adipose-derived stem cells (ADSCs) demonstrates their significant impact within the field of tissue engineering, with studies successfully demonstrating the ability to produce a range of tissue types. However, although, a broad spectrum of applications has already been suggested, many important scientific and medical questions remain unanswered before the clinical application of ADSCs in humans...
April 17, 2018: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/29667547/new-markers-for-predicting-fertility-of-the-male-gametes-in-the-post-genomic-age
#13
Savina Dipresa, Luca De Toni, Carlo Foresta, Andrea Garolla
A number of test have been proposed to assess male fertility potential, ranging from routine testing by light microscopic method for evaluating semen samples, to screening test for DNA integrity aimed to look at sperm chromatin abnormalities. Spermatozoa are an extremely differentiated cell, they have critical functions for embryo development and heredity, in addiction to delivering a haploid paternal genome to the oocyte. Towards this goal certain requirements must always be met. The ability of spermatozoa to perform its reproductive function taking place in the spermatogenesis, a highly specialized process depending on multiple factors with effect on male fertility...
April 18, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29667545/molecular-bases-of-sperm-thermotaxis-old-and-new-knowledges
#14
Luca De Toni, Savina Dipresa, Carlo Foresta, Andrea Garolla
Sperm thermotaxis, namely the temperature-oriented motility of sperm cells, is a key process involved in ejaculated sperm guidance towards the oocyte for fertilization pourposes. Since its discovery in 2003, many studies clarified the dynamic, the sensitivity and the overall weight of this process in the comprehensive context of fertilization. However, the identification of sperm thermosensor/s remained elusive until recently. The most recent cues about the molecular bases of the old known process of thermotaxis are here revised...
April 18, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29667486/lncrna-adpgk-as1-promotes-pancreatic-cancer-progression-through-activating-zeb1-mediated-epithelial-mesenchymal-transition
#15
Suzhen Song, Weihua Yu, Sen Lin, Mingbao Zhang, Teng Wang, Shuang Guo, Hongbo Wang
OBJECTIVE: This study was conducted to investigate the effects of ADP dependent glucokinase antisense RNA 1 (ADPGK-AS1)/ miR-205-5p/ zinc finger E-box binding homeobox 1 (ZEB1) on PC cells. METHODS: Differentially expressed lncRNAs and miRNAs in pancreatic cancer (PC) were identified by microarray analysis. In silico ceRNA analysis was conducted to find out the interactions among lncRNAs, miRNAs and mRNAs. Quantitative real-time PCR (qRT-PCR) was utilized to examine the expression of miR-205-5p and lncRNA ADPGK-AS1 in PC and non-cancerous cells...
April 18, 2018: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29667430/effect-of-electrolyzed-water-on-the-disinfection-of-bacillus-cereus-biofilms-the-mechanism-of-enhanced-resistance-of-sessile-cells-in-the-biofilm-matrix
#16
Mohammad Shakhawat Hussain, Minyeong Kwon, Charles Nkufi Tango, Deog Hwan Oh
This study examined the disinfection efficacy and mechanism of electrolyzed water (EW) on Bacillus cereus biofilms. B. cereus strains, ATCC 14579 and Korean Collection for Type Cultures (KCTC) 13153 biofilms, were formed on stainless steel (SS) and plastic slide (PS) coupons. Mature biofilms were treated with slightly acidic EW (SAEW), acidic EW (AEW), and basic EW (BEW). SAEW (available chlorine concentration, 25 ± 1.31 mg L-1 ; pH 5.71 ± 0.16; and oxidation reduction potential, 818 to 855 mV) reduced ATCC 14579 biofilms on plastic slides to below the detection limit within 30 s...
May 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29667316/opposite-effects-of-the-spinach-food-matrix-on-lutein-bioaccessibility-and-intestinal-uptake-lead-to-unchanged-bioavailability-compared-to-pure-lutein
#17
Marielle Margier, Caroline Buffière, Pascale Goupy, Didier Remond, Charlotte Halimi, Catherine Caris, Patrick Borel, Emmanuelle Reboul
SCOPE: Food matrix is generally believed to alter carotenoid bioavailability, but its effect on xanthophylls is usually limited. We thus aimed to decipher the digestion-absorption process of lutein in the presence or not of a food matrix. METHODS: We assessed lutein transfer to gastric-like lipid droplets or artificial mixed micelles when lutein was added to test meals either as a pure molecule ((all-E)-lutein) or in canned spinach ((Z) + (all-E)-lutein). The obtained mixed micelles were delivered to Caco-2 cells to evaluate lutein uptake...
April 17, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29667287/high-quality-cs2agbibr6-double-perovskite-film-for-lead-free-inverted-planar-heterojunction-solar-cells-with-2-2-efficiency
#18
Weiyin Gao, Chenxin Ran, Jun Xi, Bo Jiao, Wenwen Zhang, Mincai Wu, Xun Hou, Zhaoxin Wu
All inorganic double metal perovskite materials have recently drawn much attention due to their three dimensionality (3D) and non-toxic nature to replace lead based perovskite material. Among all those double perovskite materials, theoretical works have demonstrated that Cs2AgBiBr6 shows high stability and possesses suitable bandgap for solar cell application. However, the film-forming ability of Cs2AgBiBr6 is found to be the utmost challenge hindering its development in thin film solar cell device. In this work, high quality Cs2AgBiBr6 film with ultra-smooth morphology, micro-sized grains, and high crystallinity is realized via anti-solvent dropping technology and post-annealing process under high temperature...
April 17, 2018: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/29667268/a-molecular-network-for-functional-versatility-of-hecate-transcription-factors
#19
Christophe Gaillochet, Suraj Jamge, Froukje van der Wal, Gerco Angenent, Richard Immink, Jan U Lohmann
During the plant life cycle, diverse signalling inputs are continuously integrated and engage specific genetic programs depending on the cellular or developmental context. Consistent with an important role in this process, HECATE (HEC) bHLH transcription factors display diverse functions, from photomorphogenesis to the control of shoot meristem dynamics and gynoecium patterning. However, the molecular mechanisms underlying their functional versatility and the deployment of specific HEC sub-programs still remain elusive...
April 18, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29667236/recent-advances-in-matrix-assisted-laser-desorption-ionisation-mass-spectrometry-imaging-maldi-msi-for-in-situ-analysis-of-endogenous-molecules-in-plants
#20
Liang Qin, Yawen Zhang, Yaqin Liu, Huixin He, Manman Han, Yanyan Li, Maomao Zeng, Xiaodong Wang
INTRODUCTION: Mass spectrometry imaging (MSI) as a label-free and powerful imaging technique enables in situ evaluation of a tissue metabolome and/or proteome, becoming increasingly popular in the detection of plant endogenous molecules. OBJECTIVE: The characterization of structure and spatial information of endogenous molecules in plants are both very important aspects to better understand the physiological mechanism of plant organism. METHODS: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a commonly-used tissue imaging technique, which requires matrix to assist in situ detection of a variety of molecules on the surface of a tissue section...
April 17, 2018: Phytochemical Analysis: PCA
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