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https://www.readbyqxmd.com/read/28822316/improved-middle-ear-soft-tissue-visualization-using-synchrotron-radiation-phase-contrast-imaging
#1
Mai Elfarnawany, Seyed Alireza Rohani, Soroush Ghomashchi, Daniel G Allen, Ning Zhu, Sumit K Agrawal, Hanif M Ladak
High resolution images are used as a basis for finite-element modeling of the middle-ear structures to study their biomechanical function. Commonly used imaging techniques such as micro-computed tomography (CT) and optical microscopy require extensive sample preparation, processing or staining using contrast agents to achieve sufficient soft-tissue contrast. We compare imaging of middle-ear structures in unstained, non-decalcified human temporal bones using conventional absorption-contrast micro-CT and using synchrotron radiation phase-contrast imaging (SR-PCI)...
August 5, 2017: Hearing Research
https://www.readbyqxmd.com/read/28822209/primary-culture-of-rat-aortic-vascular-smooth-muscle-cells-a-new-method
#2
Jufang Chi, Liping Meng, Sunlei Pan, Hui Lin, Xiaoya Zhai, Longbin Liu, Changzuan Zhou, Chengjian Jiang, Hangyuan Guo
BACKGROUND Developing a simple and efficient method of obtaining primary cultured VSMCs is necessary for basic cardiovascular research. MATERIAL AND METHODS The procedure of our new method mainly includes 6 steps: isolation of the aortic artery, removal of the fat tissue around the artery, separation of the media, cutting the media into small tissue blocks, transferring the tissue blocks to cell culture plates, and incubation until the cells reach confluence. The cells were identified as VSMCs by morphology and immunofluorescence...
August 19, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28822182/-acute-toxicity-of-euphorbiae-pekinensis-radix-and-vinegar-processing-euphorbiae-pekinensis-radix-on-zebrafish-embryo
#3
Kai-Cheng Zhang, Yu-Dan Cao, Fang Yao, Li Zhang, An-Wei Ding
The embryos of model organism zebrafish were used to evaluate the acute toxicity of the extracts of Euphorbiae Pekinensis Radix and vinegar-processing Euphorbiae Pekinensis Radix, and the total terpene content of each extract was determined by using euphol as the reference standards. Twenty-four h normally developed zebrafish embryos were chosen, and 8 concentrations were adopted for each extract. Then the growth and death of zebrafish embryos were observed at 96 h after administration, and median lethal concentrations (LC50) of the different samples on zebrafish embryos were calculated...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822170/-effect-and-regulation-of-drying-on-quality-of-traditional-chinese-medicine-pills
#4
Ya-Ru Qi, Yuan-Hui Li, Li Han, Zhen-Feng Wu, Peng-Fei Yue, Xue-Cheng Wang, Yao-Kun Xiong, Ming Yang
The dry quality of traditional Chinese medicine pills is the hot spot of pills research, because their quality has a crucial effect on the efficacy and development of dosage forms. Through literature research and statistical analysis, we would review the current problems on the drying of traditional Chinese medicine pills in this paper, and surrounding the evaluation system for traditional Chinese medicine pills, analyze the characteristics of common drying equipment and processes as well as their effect on quality of pills, discuss the problems in drying equipment and process as well as quality, and put forward the corresponding strategies, hoping to provide new ideas and new methods for the quality improvement of traditional Chinese medicine pills and quality standards...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822161/-synergistic-mechanism-of-traditional-chinese-medicine-based-on-target-combination-of-pept1-and-ppar%C3%AE
#5
Lian-Sheng Qiao, Yan-Kun Chen, Gang-Gang Luo, Fang Lu, Si-Jia Liu, Gong-Yu Li, Yan-Ling Zhang
Synergistic effect is main pharmacological mechanism of traditional Chinese medicine(TCM). The research method based on the key targets combination is an important method to explore the synergistic effect of TCM. Peptide transporter 1 (PepT1) is an essential target for drug uptake into the bloodstream, accounting for about 50% of the total transporter protein content from the small intestine. Peroxisome proliferator-activated receptor α(PPARα) is the lipid-lowering target of fibrates, which have a good hypolipidemic effect by activating PPARα...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822147/-research-on-chemotaxis-response-of-alternaria-panax-to-amino-acid-of-ginseng-root-exudates
#6
Ai-Hua Zhang, Wen-Li Ma, Feng-Jie Lei, Ning-Bo An, Xin-Xin Zhang, Zhi-Qing Liu, Lian-Xue Zhang
Plate assay and spore germination method were used to study the chemotaxis response of Alternaria panax to arginine, glutamic acid, aspartic acid and threonine. The result showed that the optimum temperature of A. panax chemotaxis response to four amino acids were all 25 ℃. And chemotaxis responses of A. panax were different under conditions of different concentration and pH value. The chemotaxin reached to the highest under the condition of 2 mg•L⁻¹ and pH value was 7 for arginine, glutamic acid and threonine while 20 mg•L⁻¹ and pH value was 6 for aspartic acid ...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822140/optical-fiber-bragg-grating-instrumented-silicone-liner-for-interface-pressure-measurement-within-prosthetic-sockets-of-lower-limb-amputees
#7
Ebrahim Al-Fakih, Nooranida Arifin, Gholamhossein Pirouzi, Faisal Rafiq Mahamd Adikan, Hanie Nadia Shasmin, Noor Azuan Abu Osman
This paper presents a fiber Bragg grating (FBG)-instrumented prosthetic silicone liner that provides cushioning for the residual limb and can successfully measure interface pressures inside prosthetic sockets of lower-limb amputees in a simple and practical means of sensing. The liner is made of two silicone layers between which 12 FBG sensors were embedded at locations of clinical interest. The sensors were then calibrated using a custom calibration platform that mimics a real-life situation. Afterward, a custom gait simulating machine was built to test the liner performance during an amputee's simulated gait...
August 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28821841/bacterial-foraging-optimization-genetic-algorithm-for-multiple-sequence-alignment-with-multi-objectives
#8
P Manikandan, D Ramyachitra
This research work focus on the multiple sequence alignment, as developing an exact multiple sequence alignment for different protein sequences is a difficult computational task. In this research, a hybrid algorithm named Bacterial Foraging Optimization-Genetic Algorithm (BFO-GA) algorithm is aimed to improve the multi-objectives and carrying out measures of multiple sequence alignment. The proposed algorithm employs multi-objectives such as variable gap penalty minimization, maximization of similarity and non-gap percentage...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821794/a-twice-electrochemical-etching-method-to-fabricate-superhydrophobic-superhydrophilic-patterns-for-biomimetic-fog-harvest
#9
Xiaolong Yang, Jinlong Song, Junkai Liu, Xin Liu, Zhuji Jin
Superhydrophobic-superhydrophilic patterned surfaces have attracted more and more attention due to their great potential applications in the fog harvest process. In this work, we developed a simple and universal electrochemical-etching method to fabricate the superhydrophobic-superhydrophilic patterned surface on metal superhydrophobic substrates. The anti-electrochemical corrosion property of superhydrophobic substrates and the dependence of electrochemical etching potential on the wettability of the fabricated dimples were investigated on Al samples...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821618/region-specific-protein-misfolding-cyclic-amplification-reproduces-brain-tropism-of-prion-strains
#10
Nicolas Privat, Etienne Levavasseur, Serfildan Yildirim, Samia Hannaoui, Jean-Philippe Brandel, Jean-Louis Laplanche, Vincent Béringue, Danielle Seilhean, Stéphane Haïk
Human prion diseases such as Creutzfeldt-Jakob disease are transmissible brain proteinopathies, characterized by the accumulation of a misfolded isoform of the host cellular prion protein (PrP) in the brain. According to the prion model, prions are defined as proteinaceous infectious particles composed solely of this abnormal isoform of PrP (PrPSc). Even in the absence of genetic material, various prion strains can be propagated in experimental models. They can be distinguished by the pattern of disease they produce and especially by the localization of PrPSc deposits within the brain and the spongiform lesions they induce...
August 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28821551/coordinated-regulation-of-the-eii-man-and-frurki-operons-of-streptococcus-mutans-by-global-and-fructose-specific-pathways
#11
Lin Zeng, Brinta Chakraborty, Tanaz Farivar, Robert A Burne
The glucose/mannose-PTS permease EII(Man) encoded by manLMN in the dental caries pathogen Streptococcus mutans has a dominant influence on sugar-specific, CcpA-independent catabolite repression (CR). Mutations in manL affect energy metabolism and virulence-associated traits, including biofilm formation, acid tolerance and competence. Using promoter:reporter fusions, expression of the manLMN and the fruRKI operons, encoding a transcriptional regulator, a fructose-1-P kinase and a fructose-PTS permease EII(Fru), respectively, was monitored in response to carbohydrate source and in mutants lacking CcpA, FruR, and components of EII(Man) Expression of genes for EII(Man) and EII(Fru) was directly regulated by CcpA and CR, as evinced by in vivo and in vitro methods...
August 18, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28821527/systematic-review-of-economic-analyses-in-patient-safety-a-protocol-designed-to-measure-development-in-the-scope-and-quality-of-evidence
#12
Alexander W Carter, Rishi Mandavia, Erik Mayer, Joachim Marti, Elias Mossialos, Ara Darzi
INTRODUCTION: Recent avoidable failures in patient care highlight the ongoing need for evidence to support improvements in patient safety. According to the most recent reviews, there is a dearth of economic evidence related to patient safety. These reviews characterise an evidence gap in terms of the scope and quality of evidence available to support resource allocation decisions. This protocol is designed to update and improve on the reviews previously conducted to determine the extent of methodological progress in economic analyses in patient safety...
August 18, 2017: BMJ Open
https://www.readbyqxmd.com/read/28821465/polymer-lipid-hybrid-systems-used-as-carriers-for-insulin-delivery
#13
REVIEW
Anca Giorgiana Grigoras
The polymer-lipid systems successfully have been applied for loading and controlled release of insulin. These hybrid systems used the advantages of both components: enhancing of muchoadesivity and lipophilicity, respectively. Even that few polymers, but a large number of lipids were combined by different methods it is still an open field to obtain pharmaceutical formulations suitable for insulin delivery, especially by oral route. Considering that the researchers are continuously interested to find and test new materials for insulin delivery, the lipid systems (liposomes, nanoparticles, microparticles) based on natural (chitosan, lectin, ε-poly-L-lysine) or synthetic (poly(lactide-co-glycolide), poly(allylamine)) polymers were reviewed in this paper...
August 15, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28821443/the-effect-of-pelvic-motion-on-spino-pelvic-parameters
#14
Andrew M Hayden, Ann M Hayes, Jennifer L Brechbuhler, Heidi Israel, Howard M Place
BACKGROUND CONTEXT: To date, many studies have examined how pelvic position affects the spinal curvature and spino-pelvic parameters. However, these studies focus on a static relationship, comparing pelvis and spine in a relaxed or baseline position only. Indeed, the spino-pelvic connection is dynamic, as a subject can easily be taught to rotate their pelvis anteriorly or posteriorly on the femoral head, all while maintaining an erect posture. Therefore, for a true understanding of pelvic influence on the spinal column, it is necessary to examine spino-pelvic parameters in multiple pelvic positions within the same subject...
August 15, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28821437/particle-sizing-of-pharmaceutical-aerosols-via-direct-imaging-of-particle-settling-velocities
#15
Rami Fishler, Frank Verhoeven, Wilbur de Kruijf, Josué Sznitman
We present a novel method for characterizing in near real-time the aerodynamic particle size distributions from pharmaceutical inhalers. The proposed method is based on direct imaging of airborne particles followed by a particle-by-particle measurement of settling velocities using image analysis and particle tracking algorithms. Due to the simplicity of the principle of operation, this method has the potential of circumventing potential biases of current real-time particle analyzers (e.g. Time of Flight analysis), while offering a cost effective solution...
August 15, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28821095/an-o-acetyl-glucomannan-from-the-rhizomes-of-curculigo-orchioides-structural-characterization-and-anti-osteoporosis-activity-in-vitro
#16
Xueqian Wang, Mengliu Zhang, Dawei Zhang, Shumei Wang, Chunyan Yan
Osteoporosis is characterized by a reduction in bone mass and bone mineral density, which weakens the bone. Due to the side effects associated with drugs that are currently used to treat this disease, an increasing number of studies have focused on the research of effective ingredients derived from natural products. In particular, polysaccharides extracted from Chinese herbal medicines have received increasing attention. In this study, we isolated a homogeneous polysaccharide (COP90-1) from the dried rhizomes of Curculigo orchioides, a famous traditional Chinese medicine that is widely used in China, and determined its structure using the combined methods of chemical and spectral analyses...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821092/the-effect-of-temperature-on-the-colligative-properties-of-food-grade-konjac-gum-in-water-solutions
#17
Joanna Kruk, Kacper Kaczmarczyk, Anna Ptaszek, Urszula Goik, Paweł Ptaszek
This research paper presents the results of tests on the colligative properties of konjac gum chains in water solutions. For this purpose, the measurements of osmotic pressure and intrinsic viscosity of aqueous solutions, in the function of konjac gum concentration and temperature were carried out. The applied methods allowed for the determination of the second osmotic virial coefficients B2, which raised with the increase of temperature. It indicate that increase of temperature causes higher affinity of polysaccharide's chains to water...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821050/clean-approach-to-synthesis-of-graphene-like-cufe2o4-polysaccharide-resin-nanohybrid-bifunctional-compound-for-dye-adsorption-and-bacterial-capturing
#18
Mostafa Hossein Beyki, Sarina Ehteshamzadeh Ganjbakhsh, Sara Minaeian, Farzaneh Shemirani
Focus of this research is on employment of a green route to prepare magnetic CuFe2O4@polysaccharide resin for environmental remediation purpose. The method is including solvent free solid state synthesis of copper ferrite nanoparticles by combustion route using cellulose as fuel. Then polysaccharide resin as well as its magnetic composite was prepared by using glucose, citric acid and copper ferrite as solid raw materials. Characterization study using FESEM and TEM images showed that as synthesized resin possesses graphene like structure as ferrite nanoparticles are dispersed in the matrice of resin...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821007/measurement-tools-for-mental-health-problems-and-mental-well-being-in-people-with-severe-or-profound-intellectual-disabilities-a-systematic-review
#19
REVIEW
Samantha Flynn, Leen Vereenooghe, Richard P Hastings, Dawn Adams, Sally-Ann Cooper, Nick Gore, Chris Hatton, Kerry Hood, Andrew Jahoda, Peter E Langdon, Rachel McNamara, Chris Oliver, Ashok Roy, Vasiliki Totsika, Jane Waite
Mental health problems affect people with intellectual disabilities (ID) at rates similar to or in excess of the non-ID population. People with severe ID are likely to have persistent mental health problems. In this systematic review (PROSPERO 2015:CRD42015024469), we identify and evaluate the methodological quality of available measures of mental health problems or well-being in individuals with severe or profound ID. Electronic searches of ten databases identified relevant publications. Two reviewers independently reviewed titles and abstracts of retrieved records (n=41,232) and full-text articles (n=573)...
August 11, 2017: Clinical Psychology Review
https://www.readbyqxmd.com/read/28820987/a-new-label-free-electrochemical-immunosensor-based-on-dendritic-core-shell-aupd-au-nanocrystals-for-highly-sensitive-detection-of-prostate-specific-antigen
#20
Rui Wang, Wei-Dong Liu, Ai-Jun Wang, Yadong Xue, Liang Wu, Jiu-Ju Feng
Herein, bimetallic dendritic core-shell AuPd@Au nanocrystals (AuPd@Au NCs) were prepared by a simple one-pot aqueous method using xanthine as a green growth-directing agent. By virtue of the enhanced peak currents in the H2O2 reduction catalyzed by AuPd@Au NCs, a label-free immunosensor was constructed for the detection of prostate specific antigen (PSA). The immunosensor exhibited significantly improved analytical performance for the assay of PSA with wide linear range of 0.1-50ngmL(-1) and low detection limit of 0...
August 10, 2017: Biosensors & Bioelectronics
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