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https://www.readbyqxmd.com/read/29458177/colorimetric-detection-of-microrna-based-on-dnazyme-and-nuclease-assisted-catalytic-hairpin-assembly-signal-amplification
#1
Hongmei Zhang, Kuiyu Wang, Shengjun Bu, Zhongyi Li, Chuanjing Ju, Jiayu Wan
Accurate and quantitative analysis of microRNA (miRNA) expression is critical for the diagnostics and theranostics of a disease. Herein, a proof-of-concept of a colorimetric horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) biosensor for miRNA assay based on nuclease-assisted catalytic hairpin assembly (CHA) signal amplification was demonstrated. Duplex-specific nuclease (DSN) was employed to cleave the single-stranded DNA (ssDNA) chimeric probe (CP) on the magnetic bead (MB) surface via hybridization of the CP and target miRNA...
February 16, 2018: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/29458079/autofluorescence-a-potential-pitfall-in-immunofluorescence-based-inflammation-grading
#2
Jonas Wizenty, Muhammad Imtiaz Ashraf, Nadine Rohwer, Martin Stockmann, Sascha Weiss, Matthias Biebl, Johann Pratschke, Felix Aigner, Tilo Wuensch
Immunofluorescence (IF) staining of paraffin-embedded tissues is a frequently used method to answer research questions or even detect the abundance of a certain protein for diagnostic use. However, the signal originating from specific antibody-staining might be distorted by autofluorescence (AF) of the assessed tissue. Although the AF phenomenon is well known, its presence is often neglected by insufficient staining controls. In this study, we describe the existence of cellular AF in paraffin-embedded healthy and inflamed human and murine colonic tissues and present ways to reduce AF...
February 16, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29458054/comparative-investigation-of-binding-interactions-with-three-steroidal-derivatives-of-d-gggt-4-g-quadruplex-aptamer
#3
Shan Huang, Yu Liang, Jianguo Cui, Jiangning Xie, Yi Liu, Baoqing Hu, Qi Xiao
Steroidal derivatives have attracted tremendous attentions in biological and biomedical areas, due to their variety biological activities. The investigation of structural influences helps in understanding their biological activities. The interactions of steroidal derivatives with DNA may play important roles in biological activities, however only a few investigations were reported on this issue. Herein, the structural influences of three steroidal derivatives were investigated based on their binding interactions with d(GGGT)4 G-quadruplex aptamer by spectroscopic approaches, nuclear magnetic resonance (NMR), electrochemical methods, and molecular modeling techniques...
February 16, 2018: Steroids
https://www.readbyqxmd.com/read/29457850/preparation-aroma-characteristics-and-volatile-compounds-of-flavorings-from-enzymatic-hydrolyzed-rice-bran-protein-concentrate
#4
Supeeraya Arsa, Chockchai Theerakulkait
BACKGROUND: Rice bran is by-product obtained from the rice milling industry. The aims of this research were to add value of rice bran by preparation of enzymatic hydrolyzed rice bran protein concentrate (HRPC) as a flavoring agent and the flavoring which was produced by HRPC has not been discovered. Different drying methods (freeze drying and spray drying) and fructose additions were studied for improvement of rice bran protein sensorial aroma characteristics. RESULTS: the most abundant amino acids in liquid HRPC (LH) were glutamic acid, arginine, aspartic acid and leucine, respectively...
February 19, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29457793/identifying-the-favored-mutation-in-a-positive-selective-sweep
#5
Ali Akbari, Joseph J Vitti, Arya Iranmehr, Mehrdad Bakhtiari, Pardis C Sabeti, Siavash Mirarab, Vineet Bafna
Most approaches that capture signatures of selective sweeps in population genomics data do not identify the specific mutation favored by selection. We present iSAFE (for "integrated selection of allele favored by evolution"), a method that enables researchers to accurately pinpoint the favored mutation in a large region (∼5 Mbp) by using a statistic derived solely from population genetics signals. iSAFE does not require knowledge of demography, the phenotype under selection, or functional annotations of mutations...
February 19, 2018: Nature Methods
https://www.readbyqxmd.com/read/29457704/comparability-and-repeatability-of-three-commonly-used-methods-for-measuring-endurance-capacity
#6
James Baxter-Gilbert, Max Mühlenhaupt, Martin J Whiting
Measures of endurance (time to exhaustion) have been used to address a wide range of questions in ecomorphological and physiological research, as well as being used as a proxy for survival and fitness. Swimming, stationary (circular) track running, and treadmill running are all commonly used methods for measuring endurance. Despite the use of these methods across a broad range of taxa, how comparable these methods are to one another, and whether they are biologically relevant, is rarely examined. We used Australian water dragons (Intellagama lesueurii), a species that is morphologically adept at climbing, swimming, and running, to compare these three methods of endurance and examined if there is repeatability within and between trial methods...
February 19, 2018: Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology
https://www.readbyqxmd.com/read/29457694/exploring-self-management-on-glycemic-control-using-a-modified-information-motivation-behavioral-skills-model-in-type-2-diabetes-mellitus-patients-in-shanghai-china-a-cross-sectional-study
#7
Qi Chen, Huwen Wang, Yichen Wang, Zezhou Wang, DaiJun Zhao, Yong Cai
BACKGROUND: The utility and maturity of the original Information-Motivation-Behavioral Skills (IMB) model have been verified in Type 2 Diabetes Mellitus (T2DM) patients. But little research has clarified whether the model has effect on glycemic control. The main purpose of this study is to modify the IMB model to explore self-management on glycemic control in T2DM patients in Shanghai, China. METHODS: A cross-sectional study was conducted using a convenience sampling method between June and August 2015 in three tertiary hospitals and four community health service centers...
February 19, 2018: Journal of Diabetes
https://www.readbyqxmd.com/read/29457633/binding-efficiency-of-recombinant-collagen-binding-basic-fibroblast-growth-factors-cbd-bfgfs-and-their-promotion-for-nih-3t3-cell-proliferation
#8
Zhenxu Wu, Yulai Zhou, Li Chen, Mingxin Hu, Yu Wang, Linlong Li, Zongliang Wang, Peibiao Zhang
The recombinant basic fibroblast growth factor (bFGF) containing collagen-binding domain (CBD) has been found to be a potential therapeutic factor in tissue regeneration. However, its binding efficiency and quantification remain uncertain. In this research, massive recombinant bFGFs with good bioactivity for enhancing the proliferation of NIH-3T3 cells were achieved. An ELISA-based quantitative method was set up to investigate the binding efficiency of CBD-bFGFs on collagen films. It indicated that the CBDs significantly increased the collagen-binding ability of bFGF (P < ...
February 19, 2018: Biopolymers
https://www.readbyqxmd.com/read/29457539/vocalization-development-in-common-marmosets-for-neurodegenerative-translational-modeling
#9
Corinne A Jones, Mary K Duffy, Sarah A Hoffman, Nancy J Schultz-Darken, Katarina M Braun, Michelle R Ciucci, Marina E Emborg
Objectives In order to facilitate the study of vocalizations in emerging genetic common marmoset models of neurodegenerative disorders, we aimed to analyze call-type changes across age in a translational research environment. We hypothesized that acoustic parameters of vocalizations would change with age, reflecting growth of the vocal apparatus and a maturation of control needed to make adult-like calls. Methods Nineteen developing common marmosets were longitudinally video- and audio-recorded between the ages of 1-149 days in a naturalistic setting without any vocalization elicitation protocol...
February 19, 2018: Neurological Research
https://www.readbyqxmd.com/read/29457444/-effect-of-surface-modification-on-biotribological-properties-of-ultra-high-molecular-weight-polyethylene-artificial-joints
#10
REVIEW
Jie Ding, Yu-Song Pan
Friction and wear of ultra-high molecular weight polyethylene is a major cause of artificial joint failure. According to mechanism of surface modification method, friction reduction and wear-resistance properties of UHMWPE were improve by several kinds of surface modification methods. Meanwhile, this do not damage the internal structure and properties of UHMWPE. In the process, condition is easy to control and operation is simple. However, reaction time of radiation crosslinking method is too long, the material will be oxidized embrittlement; Monomer itself homopolymerization are seriously in the process of surface grafting; The injection layer of ion implantation methods is very thin and easy to be destroyed...
December 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29457443/-diagnosis-and-treatment-of-esophagustype-cervical-spondylosis
#11
REVIEW
Hao-Lin Tan, Cheng Luo, Run Zhang, Zhi-Qing Wang, Hang Ying, Hong-Ting Jin, Pei-Jian Tong, Jin-Min Liu
Esophagustype cervical spondylosis is easily misdiagnosed or missed diagnosed for its lower incidence. The pathological basis manily concerned with compression of osteophyte for esophageal and tracheal wall and local inflammatory reaction. The diagnosis of disease is generally not difficult. Especially dynamic esophagogram could not only show degree of cervical degeneration and osteophyte shape, but also could clearly observe location and degree of osteophytosis indenting esophageal and tracheal. Recently, the treatment of esophagustype cervical spondylosis has been the focus of attention...
December 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29457324/investigation-of-the-language-tasks-to-include-in-a-short-language-measure-for-children-in-the-early-school-years
#12
Jessica Matov, Fiona Mensah, Fallon Cook, Sheena Reilly
BACKGROUND: The inaccurate estimation of language difficulties by teachers suggests the benefit of a short-language measure that could be used to support their decisions about who requires referral to a speech-language therapist. While the literature indicates the potential for the development of a short-language measure, evidence is lacking about which combination of language tasks it should include. AIMS: To understand the number and nature of components/language tasks that should be included in a short-language measure for children in the early school years...
February 18, 2018: International Journal of Language & Communication Disorders
https://www.readbyqxmd.com/read/29457267/involving-children-and-young-people-in-clinical-research-through-the-forum-of-a-european-young-persons-advisory-group-needs-challenges
#13
REVIEW
Segolene Gaillard, Salma Malik, Jenny Preston, Begonya Nafria Escalera, Pamela Dicks, Nathalie Touil, Sandrine Mardirossian, Joana Claverol-Torres, Behrouz Kassaï
Children and young people are seen as fundamental to the design and delivery of clinical research as active and reflective participants. In Europe, involvement of children and young people in clinical research is promoted extensively in order to engage young people in research as partners and to give them a voice to raise their own issues or opinions and for their involvement in planning and decision making in addition to learning research skills. Children and young people can be trained in clinical research through participation in young person advisory groups (YPAGs)...
February 19, 2018: Fundamental & Clinical Pharmacology
https://www.readbyqxmd.com/read/29457241/can-rationing-through-inconvenience-be-ethical
#14
Nir Eyal, Paul L Romain, Christopher Robertson
In this article, we provide a comprehensive analysis and a normative assessment of rationing through inconvenience as a form of rationing. By "rationing through inconvenience" in the health sphere, we refer to a nonfinancial burden (the inconvenience) that is either intended to cause or has the effect of causing patients or clinicians to choose an option for health-related consumption that is preferred by the health system for its fairness, efficiency, or other distributive desiderata beyond assisting the immediate patient...
January 2018: Hastings Center Report
https://www.readbyqxmd.com/read/29457238/rationing-care-through-collaboration-and-shared-values
#15
James E Sabin
Although "rationing" continues to be a dirty word for the public in health policy discourse, Nir Eyal and colleagues handle the concept exactly right in their article in this issue of the Hastings Center Report. They correctly characterize rationing as an ethical requirement, not a moral abomination. They identify the key health policy question as how rationing can best be done, not whether it should be done at all. They make a cogent defense of what they call "rationing through inconvenience" as a justifiable allocational technique...
January 2018: Hastings Center Report
https://www.readbyqxmd.com/read/29457225/aperture-generation-based-on-threshold-segmentation-for-intensity-modulated-radiotherapy-treatment-planning
#16
Jie Yang, Zhiguo Gui, Liyuan Zhang, Pengcheng Zhang
PURPOSE: To reduce computation and decrease planning time with little or no loss in plan quality compared to the column-generation-based (cg) method, a simple method of aperture generation based on threshold segmentation (agts) is proposed. METHODS: The proposed agts strategy consists of two steps. First, a fuzzy controller is used for noise cancellation; then, new apertures are added using the threshold segmentation algorithm. We tested the proposed method on seven prostate tumor cases using the computational environment for radiotherapy research algorithms for the dose calculations and compared the results with those obtained by generating the plan aperture using the cg method...
February 19, 2018: Medical Physics
https://www.readbyqxmd.com/read/29457200/network-based-research-on-rural-opioid-use-an-overview-of-methods-and-lessons-learned
#17
REVIEW
April M Young, Abby E Rudolph, Jennifer R Havens
PURPOSE OF REVIEW: The purpose of this paper is to provide a thorough overview of methods used for recruitment, network data collection, and network data management in a network-based study of rural people who use drugs (PWUD) and to offer methodological recommendations for future research on rural drug use. RECENT FINDINGS: The Social Networks among Appalachian People (SNAP) study recruited a cohort of 503 rural PWUD via respondent-driven sampling (RDS) and has retained more than 80% of eligible participants over 7-9 years...
February 19, 2018: Current HIV/AIDS Reports
https://www.readbyqxmd.com/read/29457170/sensitive-detection-of-alkaline-phosphatase-by-dephosphorylation-initiated-transcription-reaction-mediated-dual-signal-amplification
#18
Fei Ma, Wen-Jing Liu, Li Liang, Bo Tang, Chun-Yang Zhang
We develop a new fluorescence method for the sensitive detection of alkaline phosphatase (ALP) based on dephosphorylation-initiated transcription reaction-mediated dual signal amplification. This research demonstrates for the first time a significant improvement in ALP assay performance by using a nucleic acid amplification strategy, which enables sensitive detection of ALP activity in vitro and in cell extracts. Importantly, this method can be further applied for kinetic analysis and ALP inhibition assay.
February 19, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29457147/a-scoping-review-of-health-outcomes-examined-in-randomized-controlled-trials-using-guided-imagery
#19
Peter R Giacobbi, Jonathan Stewart, Keeley Chaffee, Anna-Marie Jaeschke, Meagan Stabler, George A Kelley
Introduction: Guided imagery involves the controlled visualization of detailed mental images. This integrative health technique is used for healing, health maintenance, or the treatment of specific conditions. Guided imagery is an integral part of mindfulness meditation, hypnosis, and various relaxation exercises. However, evidence to support the widespread use and dissemination of guided imagery interventions has been lacking. The purposes of this scoping review were to document the scope of health outcomes and disease processes examined by guided imagery researchers and the journal outlets where this work has been published...
December 2017: Progress in Preventive Medicine
https://www.readbyqxmd.com/read/29456922/propensity-scores-in-pharmacoepidemiology-beyond-the-horizon
#20
John W Jackson, Ian Schmid, Elizabeth A Stuart
Purpose of review: Propensity score methods have become commonplace in pharmacoepidemiology over the past decade. Their adoption has confronted formidable obstacles that arise from pharmacoepidemiology's reliance on large healthcare databases of considerable heterogeneity and complexity. These include identifying clinically meaningful samples, defining treatment comparisons, and measuring covariates in ways that respect sound epidemiologic study design. Additional complexities involve correctly modeling treatment decisions in the face of variation in healthcare practice, and dealing with missing information and unmeasured confounding...
December 2017: Current Epidemiology Reports
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