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https://www.readbyqxmd.com/read/29451890/detection-of-bartonella-spp-in-fleas-by-maldi-tof-ms
#1
Basma El Hamzaoui, Maureen Laroche, Lionel Almeras, Jean Michel Bérenger, Didier Raoult, Philippe Parola
BACKGROUND: Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has recently emerged in the field of entomology as a promising method for the identification of arthropods and the detection of associated pathogens. METHODOLOGY/PRINCIPAL FINDINGS: An experimental model of Ctenocephalides felis (cat fleas) infected with Bartonella quintana and Bartonella henselae was developed to evaluate the efficacy of MALDI-TOF MS in distinguishing infected from uninfected fleas, and its ability to distinguish fleas infected with Bartonella quintana from fleas infected with Bartonella henselae...
February 16, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29429763/a-broad-range-pcr-technique-for-the-diagnosis-of-infective-endocarditis
#2
Ines Boujelben, Radhouane Gdoura, Adnane Hammami
Infective endocarditis (IE) remains a severe and potentially fatal disease demanding sophisticated diagnostic strategies for detection of the causative microorganisms. The aim of the present study was to develop a broad-range 16S ribosomal RNA gene polymerase chain reaction in the routine diagnostic of IE for the early diagnosis of fatal disease. A broad-range PCR technique was selected and evaluated in terms of its efficiency in the diagnosis of endocarditis using 19 heart valves from patients undergoing cardiovascular surgeries at the Habib Bourguiba Hospital of Sfax, Tunisia, on the grounds of suspected IE...
February 8, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29426471/neglected-vector-borne-zoonoses-in-europe-into-the-wild
#3
REVIEW
Laura Tomassone, Eduardo Berriatua, Rita De Sousa, Gerhard Georg Duscher, Andrei Daniel Mihalca, Cornelia Silaghi, Hein Sprong, Annetta Zintl
Wild vertebrates are involved in the transmission cycles of numerous pathogens. Additionally, they can affect the abundance of arthropod vectors. Urbanization, landscape and climate changes, and the adaptation of vectors and wildlife to human habitats represent complex and evolving scenarios, which affect the interface of vector, wildlife and human populations, frequently with a consequent increase in zoonotic risk. While considerable attention has focused on these interrelations with regard to certain major vector-borne pathogens such as Borrelia burgdorferi s...
February 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29421225/molecular-survey-on-the-occurrence-of-arthropod-borne-pathogens-in-wild-brown-hares-lepus-europaeus-from-central-italy
#4
Guido Rocchigiani, Valentina Virginia Ebani, Simona Nardoni, Fabrizio Bertelloni, Alice Bascherini, Alessandro Leoni, Francesca Mancianti, Alessandro Poli
Data about the spreading of arthropod-borne pathogens among hare populations are very scant, so the aim of the present preliminary study was to investigate, through molecular analysis, the occurrence of Anaplasma phagocytophilum, Bartonella sp., Borrelia burgdorferi sensu lato, Coxiella burnetii, Francisella tularensis, Leishmania spp. and piroplasms DNA in blood of 51 wild hares (Lepus europaeus) living in protected areas in Tuscany. All hares resulted negative for A. phagocytophilum, Bartonella sp., B. burgdorferi s...
February 5, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29418086/molecular-diagnosis-of-skin-infections-using-paraffin-embedded-tissue-review-and-interdisciplinary-consensus
#5
REVIEW
Cord Sunderkötter, Karsten Becker, Heinz Kutzner, Thomas Meyer, Norbert Blödorn-Schlicht, Udo Reischl, Pietro Nenoff, Walter Geißdörfer, Yvonne Gräser, Mathias Herrmann, Joachim Kühn, Christian Bogdan
Nucleic acid amplification techniques (NATs), such as PCR, are highly sensitive and specific methods that have become valuable supplements to culture and serology in the diagnosis of infectious disorders. However, especially when using formalin-fixed and paraffin-embedded tissue, these techniques are associated with both false-negative and false-positive results, a pitfall that is frequently misjudged. Representatives of the German Society of Hygiene and Microbiology (DGHM) and the German Society of Dermatology (DDG) therefore set out to develop a consensus - in the form of a review article - on the appropriate indications for NATs using paraffin-embedded tissue, its contraindications, and the key points to be considered in the pre- and post-analytical phase...
February 2018: Journal der Deutschen Dermatologischen Gesellschaft, Journal of the German Society of Dermatology: JDDG
https://www.readbyqxmd.com/read/29406284/isolation-and-phylogenetic-analysis-of-bartonella-species-from-rusa-deer-rusa-timorensis-in-thailand
#6
Decha Pangjai, Santaya Intachinda, Soichi Maruyama, Sumalee Boonmar, Hidenori Kabeya, Shingo Sato, Wimol Petkanchanapong, Wattanapong Wootta, Piyada Wangroongsarb, Maskiet Boonyareth, Watcharee Saisongkorh, Poom Preedakoon, Panupong Mahaprom, Chonlada Meesub, Pathom Sawanpanyalert
The aim of the present study is to investigate the prevalence of Bartonella infection in deer in Thailand and to characterize the isolates by biochemical, morphological and genetic analysis. A total of 247 blood samples were collected from Rusa deer (Rusa timorensis) in a livestock breeding facility in Thailand. Bartonella bacteria were isolated in 3.6% of the blood samples. Three out of 110 (2.7%) males and 6 of 137 (4.4%) females were positive for Bartonella. A higher prevalence of Bartonella was observed in young deer under 4 years of age compared to adults over 4 years of age, but no Bartonella was isolated from deer over 8 years of age...
February 2018: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/29397399/longitudinal-effects-of-supplemental-forage-on-the-honey-bee-apis-mellifera-microbiota-and-inter-and-intra-colony-variability
#7
Jason A Rothman, Mark J Carroll, William G Meikle, Kirk E Anderson, Quinn S McFrederick
Honey bees (Apis mellifera) provide vital pollination services for a variety of agricultural crops around the world and are known to host a consistent core bacterial microbiome. This symbiotic microbial community is essential to many facets of bee health, including likely nutrient acquisition, disease prevention and optimal physiological function. Being that the bee microbiome is likely involved in the digestion of nutrients, we either provided or excluded honey bee colonies from supplemental floral forage before being used for almond pollination...
February 3, 2018: Microbial Ecology
https://www.readbyqxmd.com/read/29393980/bartonella-henselae-infection-in-a-dog-with-recalcitrant-ineffective-erythropoiesis
#8
Martin G Randell, Nandhakumar Balakrishnan, Rebekah Gunn-Christie, Andrew Mackin, Edward B Breitschwerdt
Ineffective erythropoiesis was diagnosed in an 8-year-old male castrated Labrador Retriever. Despite treatment with immunosuppressive therapy for suspected immune-mediated erythrocyte maturation arrest, resolution of the nonregenerative anemia was not achieved. Following documentation of Bartonella henselae bacteremia by Bartonella alpha proteobacteria growth medium (BAPGM) enrichment blood culture, immunosuppressive therapy was discontinued, and the anemia resolved following prolonged antibiotic therapy. Bartonella immunofluorescent antibody testing was negative, whereas B henselae western blot was consistently positive...
February 2, 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/29390335/fever-of-unknown-origin-and-splenomegaly-a-case-report-of-blood-culture-negative-endocarditis
#9
Maria Livia Burzo, Mariangela Antonelli, Giovanni Pecorini, Angela M R Favuzzi, Raffaele Landolfi, Andrea Flex
RATIONALE: Fever of unknown origin (FUO) can be determined by different conditions among which infectious diseases represent the main cause. PATIENT CONCERNS: A young woman, with a history of aortic stenosis, was admitted to our unit for a month of intermittent fever associated with a new diastolic heart murmur and splenomegaly. Laboratory tests were negative for infectious screening. The total body computed tomography (CT) scan excluded abscesses, occulted neoplasia, or lymphadenopathy...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29381832/do-not-disregard-diagnostic-clues-of-endocarditis-comment-on-the-article-by-garg-et-al
#10
Claude Bachmeyer, Victoire Dubourdieu, Barthélémy Poignet
We read with great interest the Clinicopathologic Conference by Garg et al. published recently in Arthritis Care & Research about a patient with Bartonella-related endocarditis (1). However, we regret that the 'petechial pruritic rash' on the lower extremities and the presence of elevated rheumatoid factor were initially neglected in a context of glomerulonephritis. Indeed the rash was poorly described. This article is protected by copyright. All rights reserved.
January 30, 2018: Arthritis Care & Research
https://www.readbyqxmd.com/read/29369724/identification-of-hemotropic-mycoplasmas-in-an-eastern-box-turtle-terrapene-carolina-carolina-and-a-yellow-bellied-slider-trachemys-scripta-scripta-from-north-carolina
#11
Jo Jarred, Gregory Lewbart, Kelsey Stover, Brittany Thomas, Ricardo Maggi, Edward B Breitschwerdt
  Mycoplasma spp. are known from several chelonian and other reptilian species. We determined if turtles obtained by the Turtle Rescue Team at North Carolina State University are carriers of hemotropic Mycoplasma or Bartonella spp. Spleen samples were collected at necropsy during May through July, 2014 from 53 turtles of seven species. All turtles were dead or were euthanized upon arrival due to severe traumatic injuries, or they died shortly after beginning treatment. We used PCR amplification for both bacterial genera; Bartonella spp...
January 25, 2018: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/29355984/pcr-evaluation-of-selected-vector-borne-pathogens-in-dogs-with-pericardial-effusion
#12
M-D Tabar, R Movilla, L Serrano, L Altet, O Francino, X Roura
OBJECTIVES: To investigate evidence for selected vector-borne pathogen infections in dogs with pericardial effusion living in a Mediterranean area in which several canine vector-borne diseases are endemic. MATERIALS AND METHODS: Archived EDTA blood (n=68) and pericardial fluid samples (n=58) from dogs with pericardial effusion (n=68) were included. Dogs without pericardial effusion examined for other reasons were included as controls (n=60). Pericardial effusion was classified as neoplastic in 40 dogs, idiopathic in 23 dogs and of unknown aetiology in 5 dogs...
January 22, 2018: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/29336912/-bartonella-henselae-serological-evidence-in-pediatric-patients-with-clinical-suspicion-of-cat-scratch-disease
#13
Rita Armitano, Agustina Lisa, Claudia Martínez, Lucia Cipolla, Ricardo Iachini, Monica Prieto
Cat scratch disease (CSD) is caused by Bartonella henselae, which mainly affects children. The cat is the reservoir. The laboratory diagnosis is based on the detection of antibodies by the Indirect Immunofluorescence (IFI) assay. The objective of this study was to analyze the serological evidence of B. henselae infection in pediatric patients that met the clinical/epidemiological criteria for suspected CSD. We studied 92 patients, who were categorized into four serological groups: 1) IgG (+)/IgM(+), 31,5% (n=29); 2) IgG (-)/IgM(+), 10,9% (n=10); 3) IgG (+)/IgM(-), 9,8% (n=9); 4) IgG (-)/IgM(-), 47,8% (n=44)...
January 11, 2018: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29329460/survey-of-parasitic-bacteria-in-bat-bugs-colorado
#14
Clifton D McKee, Lynn M Osikowicz, Teresa R Schwedhelm, Ying Bai, Kevin T Castle, Michael Y Kosoy
Bat bugs (Cimex adjunctus Barber) (Hemiptera: Cimicidae) collected from big brown bats (Eptesicus fuscus Palisot de Beauvoir) in Colorado, United States were assessed for the presence of Bartonella, Brucella, and Yersinia spp. using molecular techniques. No evidence of Brucella or Yersinia infection was found in the 55 specimens collected; however, 4/55 (7.3%) of the specimens were positive for Bartonella DNA. Multi-locus characterization of Bartonella DNA shows that sequences in bat bugs are phylogenetically related to other Bartonella isolates and sequences from European bats...
January 10, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29326218/first-draft-genome-sequences-of-two-bartonella-tribocorum-strains-from-laos-and-cambodia
#15
Linda Hadjadj, Tawisa Jiyipong, Fadi Bittar, Serge Morand, Jean-Marc Rolain
Bartonella tribocorum is a Gram-negative bacterium known to infect animals, and rodents in particular, throughout the world. In this report, we present the draft genome sequences of two strains of B. tribocorum isolated from the blood of a rodent in Laos and a shrew in Cambodia.
January 11, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29316554/skin-infections-due-to-bacteria-in-solid-organ-transplant-recipients-a-review
#16
Muneeb Ilyas, Nishita Maganty, Zachary Ginsberg, Amit Sharma
Though there is an abundance of information on cutaneous malignancies in transplant recipients, cutaneous infections in solid organ transplant recipients (SOTRs) are underrepresented in the dermatological literature. Our paper provides a comprehensive review of bacterial cutaneous infections within the solid organ transplant population. Cutaneous bacterial infections may lead to significant morbidity and even mortality in this immunosuppressed population. Thus, it is to the benefit of both dermatologists and other transplant care providers to better understand and recognize the features of cutaneous bacterial infections in SOTRs...
January 10, 2018: Dermatology: International Journal for Clinical and Investigative Dermatology
https://www.readbyqxmd.com/read/29313064/genetic-diversity-of-bartonella-spp-in-wild-mammals-and-ectoparasites-in-brazilian-pantanal
#17
Keyla Carstens Marques de Sousa, Renan Bressianini do Amaral, Heitor Miraglia Herrera, Filipe Martins Santos, Gabriel Carvalho Macedo, Pedro Cordeiro Estrela de Andrade Pinto, Darci Moraes Barros-Battesti, Rosangela Zacarias Machado, Marcos Rogério André
The present work aimed to investigate the genetic diversity of Bartonella in mammals and ectoparasites in Pantanal wetland, Brazil. For this purpose, 31 Nasua nasua, 78 Cerdocyon thous, 7 Leopardus pardalis, 110 wild rodents, 30 marsupials, and 42 dogs were sampled. DNA samples were submitted to a quantitative real-time PCR assay (qPCR). Positive samples in qPCR were submitted to conventional PCR assays targeting other five protein-coding genes. Thirty-five wild rodents and three Polygenis (P.) bohlsi bohlsi flea pools showed positive results in qPCR for Bartonella spp...
January 8, 2018: Microbial Ecology
https://www.readbyqxmd.com/read/29311425/phylogeny-and-putative-virulence-gene-analysis-of-bartonella-bovis
#18
Sun Tee Tay, Kai Ling Kho, Siew Fen Lye, Yun Fong Ngeow
Bartonella bovis is a small Gram-negative bacterium recognized as an etiological agent for bacteremia and endocarditis in cattle. As few reports are available on the taxonomic position of B. bovis and its mechanism of virulence, this study aims to resolve the phylogeny of B. bovis and investigate putative virulence genes based on whole genome sequence analysis. Genome-wide comparisons based on single nucleotide polymorphisms (SNP) and orthologous genes were performed in this study for phylogenetic inference of 27 Bartonella species...
December 29, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29298408/exposure-to-rats-and-rat-associated-leptospira-and-bartonella-species-among-people-who-use-drugs-in-an-impoverished-inner-city-neighborhood-of-vancouver-canada
#19
David A McVea, Chelsea G Himsworth, David M Patrick, L Robbin Lindsay, Michael Kosoy, Thomas Kerr
Rat infestations are common, particularly in impoverished, inner-city neighborhoods. However, there has been little research into the nature and consequences of rat exposure in these neighborhoods, particularly in Canada. In this study, we sought to characterize exposure to rats and rat-associated Leptospira interrogans and Bartonella tribocorum, as well as risk factors associated with exposure, in residents (n = 202) of the Downtown Eastside (DTES) neighborhood of Vancouver, Canada. There was no evidence of exposure to rat-associated L...
January 3, 2018: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/29287070/a-diagnostic-and-epidemiologic-investigation-of-acute-febrile-illness-afi-in-kilombero-tanzania
#20
Christine Hercik, Leonard Cosmas, Ondari D Mogeni, Newton Wamola, Wanze Kohi, Victor Omballa, Melvin Ochieng, Shirley Lidechi, Juma Bonventure, Caroline Ochieng, Clayton Onyango, Barry S Fields, Sayoki Mfinanga, Joel M Montgomery
INTRODUCTION: In low-resource settings, empiric case management of febrile illness is routine as a result of limited access to laboratory diagnostics. The use of comprehensive fever syndromic surveillance, with enhanced clinical microbiology, advanced diagnostics and more robust epidemiologic investigation, could enable healthcare providers to offer a differential diagnosis of fever syndrome and more appropriate care and treatment. METHODS: We conducted a year-long exploratory study of fever syndrome among patients ≥ 1 year if age, presenting to clinical settings with an axillary temperature of ≥37...
2017: PloS One
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