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Tsutomu Mito, Yusuke Hirota, Shingo Suzuki, Kazutaka Noda, Takanori Uehara, Yoshiyuki Ohira, Masatomi Ikusaka
A 65-year-old Japanese man was admitted with a 4-month history of fatigue and exertional dyspnea. Transthoracic echocardiography revealed a vegetation on the aortic valve and severe aortic regurgitation. Accordingly, infective endocarditis and heart failure were diagnosed. Although a blood culture was negative on day 7 after admission, a prolonged blood culture with subculture was performed according to the patient's history of contact with cats. Consequently, Bartonella henselae was isolated. Bartonella species are fastidious bacteria that cause blood culture-negative infective endocarditis...
2016: Internal Medicine
Elena Parra, Ferran Segura, Jessica Tijero, Imma Pons, Maria-Mercedes Nogueras
A real-time PCR assay using SYBR Green was optimized to detect those Bartonella that are most frequently described as pathogens. The assay was genus-specific. Sequencing allowed to distinguish species. Assay sensitivity was determined using 10-fold serial dilutions of genomic DNA. Dynamic range was 100 ng-100 fg and sensitivity was 50 copies/reaction.
October 13, 2016: Molecular and Cellular Probes
Luiz Ricardo Gonçalves, Alexsandra Rodrigues de Mendonça Favacho, André Luiz Rodrigues Roque, Natalia Serra Mendes, Otávio Luiz Fidelis Junior, Jyan Lucas Benevenute, Heitor Miraglia Herrera, Paulo Sérgio D'Andrea, Elba Regina Sampaio de Lemos, Rosangela Zacarias Machado, Marcos Rogério André
: Bartonella spp. comprise an ecologically successful group of microorganisms that infects erythrocytes and have adapted to different hosts, which include a wide range of mammals, besides humans. Rodents are reservoirs of about two thirds of Bartonella spp. described to date; and some of them have been implicated as causative agents of human diseases. In our study, we performed the molecular and phylogenetic analyses of Bartonella spp. infecting wild rodents from five different Brazilian biomes...
October 7, 2016: Applied and Environmental Microbiology
Jan Hubert, Martina Bicianova, Ondrej Ledvinka, Martin Kamler, Philip J Lester, Marta Nesvorna, Jan Kopecky, Tomas Erban
The honey bee, Apis mellifera, is a globally important species that suffers from a variety of pathogens and parasites. These parasites and pathogens may have sublethal effects on their bee hosts via an array of mechanisms, including through a change in symbiotic bacterial taxa. Our aim was to assess the influence of four globally widespread parasites and pathogens on the honey bee bacteriome. We examined the effects of the ectoparasitic mite Varroa destructor, the fungal pathogens Nosema apis and Nosema ceranae, and the trypanosome Lotmaria passim...
October 11, 2016: Microbial Ecology
Didier Raoult
We have been involved in the field of paleomicrobiology since 1998, when we used dental pulp to identify Yersinia pestis as the causative agent of the great plague of Marseille (1720). We recently designed a specific technique, "suicide PCR," that can prevent contamination. A controversy arose between two teams, with one claiming that DNA must be altered to amplify it and the other group claiming that demographic data did not support the role of Y. pestis in the Black Death (i.e., the great plague of the Middle Ages)...
August 2016: Microbiology Spectrum
Lynn M Osikowicz, Sarah A Billeter, Maria Fernanda Rizzo, Michael P Rood, Ashley N Freeman, Joseph E Burns, Renjie Hu, Phalasy Juieng, Vladimir Loparev, Michael Kosoy
We investigated the prevalence of Bartonella washoensis in California ground squirrels (Otospermophilus beecheyi) and their fleas from parks and campgrounds located in seven counties of California. Ninety-seven of 140 (69.3%) ground squirrels were culture positive and the infection prevalence by location ranged from 25% to 100%. In fleas, 60 of 194 (30.9%) Oropsylla montana were found to harbor Bartonella spp. when screened using citrate synthase (gltA) specific primers, whereas Bartonella DNA was not found in two other flea species, Hoplopsyllus anomalus (n = 86) and Echidnophaga gallinacea (n = 6)...
October 5, 2016: Vector Borne and Zoonotic Diseases
Gosia Malgorzata-Miller, Lena Heinbockel, Klaus Brandenburg, Jos W M van der Meer, Mihai G Netea, Leo A B Joosten
The pattern recognition receptor TLR4 is well known as a crucial receptor during infection and inflammation. Several TLR4 antagonists have been reported to inhibit the function of TLR4. Both natural occurring antagonists, lipopolysaccharide (LPS) from Gram-negative bacteria as well as synthetic compounds based on the lipid A structure of LPS have been described as potent inhibitors of TLR4. Here, we have examined the characteristics of a natural TLR4 antagonist, isolated from Bartonella quintana bacterium by elucidating its chemical primary structure...
September 27, 2016: Scientific Reports
Cláudia Gomes, Sandra Martínez-Puchol, Lidia Ruiz-Roldán, Maria J Pons, Juana Del Valle Mendoza, Joaquim Ruiz
The objective was to develop and characterise in vitro Bartonella bacilliformis antibiotic resistant mutants. Three B. bacilliformis strains were plated 35 or 40 times with azithromycin, chloramphenicol, ciprofloxacin or rifampicin discs. Resistance-stability was assessed performing 5 serial passages without antibiotic pressure. MICs were determined with/without Phe-Arg-β-Napthylamide and artesunate. Target alterations were screened in the 23S rRNA, rplD, rplV, gyrA, gyrB, parC, parE and rpoB genes. Chloramphenicol and ciprofloxacin resistance were the most difficult and easiest (>37...
September 26, 2016: Scientific Reports
David Dornbos, Jocelyn Morin, Joshua R Watson, Jonathan Pindrik
Osteomyelitis of the spine with associated spinal epidural abscess represents an uncommon entity in the pediatric population, requiring prompt evaluation and diagnosis to prevent neurological compromise. Cat scratch disease, caused by the pathogen Bartonella henselae, encompasses a wide spectrum of clinical presentations; however, an association with osteomyelitis and epidural abscess has been reported in only 4 other instances in the literature. The authors report a rare case of multifocal thoracic osteomyelitis with an epidural abscess in a patient with a biopsy-proven pathogen of cat scratch disease...
September 23, 2016: Journal of Neurosurgery. Pediatrics
Amy K Wray, Kevin J Olival, David Morán, Maria Renee Lopez, Danilo Alvarez, Isamara Navarrete-Macias, Eliza Liang, Nancy B Simmons, W Ian Lipkin, Peter Daszak, Simon J Anthony
Certain bat species serve as natural reservoirs for pathogens in several key viral families including henipa-, lyssa-, corona-, and filoviruses, which may pose serious threats to human health. The Common Vampire Bat (Desmodus rotundus), due to its abundance, sanguivorous feeding habit involving humans and domestic animals, and highly social behavioral ecology, may have an unusually high potential for interspecies disease transmission. Previous studies have investigated rabies dynamics in D. rotundus, yet the diversity of other viruses, bacteria, and other microbes that these bats may carry remains largely unknown...
September 22, 2016: EcoHealth
Julien Saison, Brahim Harbaoui, Coralie Bouchiat, Matteo Pozzi, Tristan Ferry
No abstract text is available yet for this article.
2016: BMJ Case Reports
Hélio Galdino, Rodrigo Saar Gomes, Jessica Cristina Dos Santos, Lívia Lara Pessoni, Anetícia Eduarda Maldaner, Stéfanne Madalena Marques, Clayson Moura Gomes, Miriam Leandro Dorta, Milton Adriano Pelli de Oliveira, Leo A B Joosten, Fátima Ribeiro-Dias
While the role of Toll-like receptors (TLRs) has been investigated in murine models of tegumentary leishmaniasis caused by Leishmania (Viannia) braziliensis, the interaction between TLRs and Leishmania sp. has not been investigated in human cells. The aim of this study was to evaluate the involvement of TLR4 in cytokine production of human peripheral blood mononuclear cells (PBMCs) induced by L. braziliensis, and whether the parasite alters the expression of TLR4 on monocytes/macrophages. Amastigote forms were obtained from mice lesions and PBMCs were isolated from healthy donors...
December 2016: Cytokine
Djamel Tahir, Cristina Socolovschi, Jean-Lou Marié, Gautier Ganay, Jean-Michel Berenger, Jean-Michel Bompar, Denis Blanchet, Marie Cheuret, Oleg Mediannikov, Didier Raoult, Bernard Davoust, Philippe Parola
In French Guiana, located on the northeastern coast of South America, bats of different species are very numerous. The infection of bats and their ticks with zoonotic bacteria, especially Rickettsia species, is so far unknown. In order to improve knowledge of these zoonotic pathogens in this French overseas department, the presence and diversity of tick-borne bacteria was investigated with molecular tools in bat ticks. In the beginning of 2013, 32 bats were caught in Saint-Jean-du-Maroni, an area close to the coast of French Guiana, and the ticks of these animals were collected...
October 2016: Ticks and Tick-borne Diseases
Cláudia Gomes, Noemí Palma, Maria J Pons, Ariel Magallón-Tejada, Isabel Sandoval, Carmen Tinco-Valdez, Carlos Gutarra, Juana Del Valle-Mendoza, Joaquim Ruiz, Mayumi Matsuoka
BACKGROUND: Bartonella bacilliformis is the causative agent of Carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. The lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. This study aimed to identify new B. bacilliformis antigenic candidates that could lead to a new diagnostic tool able to be implemented in endemic rural areas. METHODOLOGY/PRINCIPAL FINDINGS: Blood (n = 198) and serum (n = 177) samples were collected in northern Peru...
September 2016: PLoS Neglected Tropical Diseases
Frederique B Schulte Fischedick, Matthew J Stuckey, Alvaro Aguilar-Setién, Hayde Moreno-Sandoval, Guillermo Galvez-Romero, Mónica Salas-Rojas, Nidia Arechiga-Ceballos, Paul A M Overgaauw, Rickie W Kasten, Bruno B Chomel
Bartonella species are highly endemic among wild rodents in many parts of the world. Blood and/or blood clot cultures from 38 rodents, including 27 Yucatan deer mouse (Peromyscus yucatanicus), 7 Gaumer's spiny pocket mouse (Heteromys gaumeri), 2 black rats (Rattus rattus) and 2 big-eared climbing rats (Ototylomys phyllotis) captured near Merida, Yucatan, Mexico, led to the isolation in 3-4 days of small gram-negative bacilli, which were identified as Bartonella spp. based on colony morphology. DNA extraction and PCR testing were also performed from heart samples of 35 of these 38 rodents...
October 2016: Vector Borne and Zoonotic Diseases
Ignacio Troncoso, Christof Fischer, Francisca Arteaga, Cristian Espinoza, Teresa Azócar, Katia Abarca
INTRODUCTION: Bartonella henselae infection is a worldwide zoonosis with the domestic cat as reservoir. Although people with occupational contact with these pets are risk population only few studies of prevalence in them have been reported. METHODS: A study of seroprevalence of B. henselae was performed to veterinaries and other persons with occupational contact with cats, residents from the Bío-Bío region of Chile. Serum IgG antibodies against B. henselae were determined by indirect immunofluorescence (IFI)...
June 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
E Rossi, A Perrone, D Narese, M Cangelosi, S Sollai, A Semeraro, M Mortilla, C Defilippi
Cat-scratch disease (CSD) is a zoonosis in children, result of infection by Bartonella henselae, a gram-negative bacillus. Infection is generally characterized by regional and self-limited lymphadenopathy after exposure to a scratch or bite from a cat. Rarely, B. henselae is cause of fever of unknown origin (FUO), with dissemination to various organs, most often involving the reticuloendothelial system (liver, spleen, bone marrow), mimicking an inflammatory rather than a lymphoproliferative disease. Whole-body Magnetic Resonance Imaging (WBMRI), in association with diffusion-weighted imaging (DWIBS), allows a comprehensive evaluation of pediatric patients, without the risks inherent to ionizing radiation...
July 2016: La Clinica Terapeutica
Suheir Ereqat, Abdelmajeed Nasereddin, Muriel Vayssier-Taussat, Ahmad Abdelkader, Amer Al-Jawabreh, Taher Zaid, Kifaya Azmi, Ziad Abdeen
Ticks play an important role in disease transmission as vectors for human and animal pathogens, including the Gram-negative pathogen Bartonella. Here, we evaluated the presence of Bartonella in ixodid ticks and domestic animals from Palestine. We tested 633 partly engorged ticks and 139 blood samples from domestic animals (dogs, sheep and camels) for Bartonella using ITS-PCR. Bartonella DNA was detected in 3.9% of the tested ticks. None of the ticks collected from sheep and goats were positive for Bartonella...
2016: Frontiers in Microbiology
Ying Bai, Amy Gilbert, Karen Fox, Lynn Osikowicz, Michael Kosoy
Spleen samples from 292 wild carnivores from Colorado, US were screened for Bartonella infection. Bartonella DNA was detected in coyotes ( Canis latrans ) (28%), striped skunks ( Mephitis mephitis ) (23%), red foxes ( Vulpes vulpes ) (27%), and raccoons ( Procyon lotor ) (8%) but not in black bears ( Ursus americanus ), gray foxes ( Urocyon cinereoargenteus ), and mountain lions ( Puma concolor ). Two Bartonella species, B. vinsonii subsp. berkhoffii and B. rochalimae, were identified. All 10 infected striped skunks exclusively carried B...
October 2016: Journal of Wildlife Diseases
Antonio Zurita, Sara García Gutiérrez, Cristina Cutillas
In the present study, a molecular detection of Bartonella sp. and Wolbachia sp. in Ctenocephalides felis (Siphonaptera: Pulicidae) isolated from Canis lupus familiaris from different geographical areas of Spain, Iran and South Africa, and in Stenoponia tripectinata tripectinata isolated from Mus musculus from the Canary Islands has been carried out by amplification of the 16S ribosomal RNA partial gene of Wolbachia sp. and intergenic spacer region (its region) of Bartonella sp. A total of 70 % of C. felis analysed were infected by W...
November 2016: Current Microbiology
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